Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,744,506 C→A 27.6% G241G (GGG→GGT)  yiaT ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,744,5060CA27.6% 455.4 / 173.3 223G241G (GGG→GGT) yiaTputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (84/77);  new base A (29/33);  total (113/110)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

AAGGCAAGGGATTGTTCAGCCATAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCACCCAGGC  >  CP009273/3744357‑3744655
                                                                                                                                                     |                                                                                                                                                     
aaGGCAAGGGATTGTTCAGCCATAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCAcc                                                                                                                                                       >  1:564051/1‑150 (MQ=255)
      tggttttGTACTGCCATATTATCCTTATTCAGTCGCTTGGTTCCCGGTTTGACGATAGCAATCCAGAGCAAAAACGTTTGTGGCTTTACGACGGAGCACCTTCCGCACGGTCATACAGCGGTGAATTAAAAACGCCAGCTCACACCGGTg                                                                                                                                                 <  2:4865290/145‑1 (MQ=255)
        gttttGTTCAGCCATAAGATCCTGATTCAGACGCCTGGTTCCCGTTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGAATTACGACGGAGCACCAGCCGCACCGACATCCCCCGGTGAATTAAAAACGCCAGCTCACACCGGTGAc                                                                                                                                               <  1:4952288/147‑1 (MQ=255)
             tttaaGCCATACTATCCTTATTCTGCCGCCTGGTTCCCGTTTTGAACTGAGAAATCCAGAGCAAAAACATTTGGGTAATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTTAATTAAAAACGCCAGCTCACCCCGGTGACAAAgg                                                                                                                                          <  1:1225053/146‑1 (MQ=255)
             gTTCAGCCATAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAgg                                                                                                                                          >  2:2780854/1‑150 (MQ=255)
             gTTCAGCCATAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAgg                                                                                                                                          >  2:1890250/1‑150 (MQ=255)
                cAGCCATAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTaa                                                                                                                                       <  2:4651205/150‑1 (MQ=255)
                  gCCATAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTaaaa                                                                                                                                     >  2:3605167/1‑150 (MQ=255)
                   tcATAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAAc                                                                                                                                    >  2:1062692/2‑150 (MQ=255)
                    cATAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGACTTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAAct                                                                                                                                   >  2:3896180/1‑150 (MQ=255)
                     aTAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCATAGCAAAAACCTTTGTGGCATTACGACGGAGCACCAGCCGCCCCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAActc                                                                                                                                  <  2:4355578/150‑1 (MQ=255)
                     aTAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAActc                                                                                                                                  >  2:1446566/1‑150 (MQ=255)
                     aTAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAActc                                                                                                                                  >  1:2764690/1‑150 (MQ=255)
                     aTAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAActc                                                                                                                                  <  2:277966/150‑1 (MQ=255)
                          aTCCTGATTCAGACGCCTGGTGCCCGGTATGAACAGAGCAATCCAGAGAAACAACCTTTGTGGAATTACGACGGAGCACCCGCCGCACCGAAATCCACCGTTTAATAAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCAtt                                                                                                                             <  1:4501321/150‑1 (MQ=255)
                             cTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACg                                                                                                                          >  2:1743312/1‑150 (MQ=255)
                                aTTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGatc                                                                                                                       <  1:864137/150‑1 (MQ=255)
                                 ttCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGatca                                                                                                                      <  2:2882908/150‑1 (MQ=255)
                                 ttCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGatca                                                                                                                      >  2:518276/1‑150 (MQ=255)
                                   aaGACTCCAGGTACCCGTTTTTAATATAGCATTCCATCGCAACAACTTTTGTGGCTTTACGAGGGATCACCTGCCGAAACGAAATCCACCGGTTAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGatcaat                                                                                                                    <  2:3184299/149‑1 (MQ=255)
                                     gACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGatcaatca                                                                                                                  >  1:2781047/1‑150 (MQ=255)
                                      aCGCCTGGTACCCGGTATGAACAGATAAATCAAGACAAACAACCTTTGTGTCATTACGACTGAGCACCAGCCCCACCCAAATCAACCGTTTAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAatcat                                                                                                                 <  2:2226115/150‑1 (MQ=255)
                                      aCGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACACAGGTAAAACTCTCATTACGATCAatcat                                                                                                                 >  2:4250869/1‑150 (MQ=255)
                                      aCGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAatcat                                                                                                                 >  2:1651123/1‑150 (MQ=255)
                                        gCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCg                                                                                                               >  2:2532835/1‑150 (MQ=255)
                                        gCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCg                                                                                                               >  1:2589352/1‑150 (MQ=255)
                                        gCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCg                                                                                                               >  2:3236583/1‑150 (MQ=255)
                                         catggTTCCGGGTATGAACTTTGAAATCCAGAGCAACAACGTTTTTGGAATTACGAGGGTGCACCAGCCGCACCTACTTCCACCGGTTAATTAAACACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCgg                                                                                                              <  1:5060872/147‑1 (MQ=255)
                                          cAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCggg                                                                                                             >  2:2863227/1‑150 (MQ=255)
                                            ggTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCt                                                                                                           >  2:520510/1‑150 (MQ=255)
                                             gTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTg                                                                                                          <  2:3373437/150‑1 (MQ=255)
                                              ttCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGt                                                                                                         <  2:407528/150‑1 (MQ=255)
                                                     tATTAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAAt                                                                                                  <  2:2333180/150‑1 (MQ=255)
                                                     tATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAAt                                                                                                  >  2:4996792/1‑150 (MQ=255)
                                                     tATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAAt                                                                                                  <  2:2068427/150‑1 (MQ=255)
                                                      aTGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAAtc                                                                                                 >  1:4929749/1‑150 (MQ=255)
                                                         aaCATAGCAATCCACAGCAACAACATTTGTGGAATTCCGACGGACCACCCGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATctct                                                                                              <  1:4038036/150‑1 (MQ=255)
                                                         aaCAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATctct                                                                                              >  2:4179001/1‑150 (MQ=255)
                                                             gagCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCCGCCGCCCCGACACCCCCCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCg                                                                                          <  2:4406704/150‑1 (MQ=255)
                                                              agCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCgg                                                                                         >  2:2240785/1‑150 (MQ=255)
                                                                cAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCa                                                                                       >  1:2163716/1‑150 (MQ=255)
                                                                   tCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCt                                                                                    >  1:1044955/1‑150 (MQ=255)
                                                                   tCCAGACAAACACCCTTTGTGGCATAACGCCGGAGCACCCGCCCCACCGACACCCCCCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCt                                                                                    <  2:1321823/150‑1 (MQ=255)
                                                                    ccAGAGCAACACCCTTTGTGGCATTACGCCGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTc                                                                                   <  1:1228096/150‑1 (MQ=255)
                                                                    ccAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTc                                                                                   >  2:5134381/1‑150 (MQ=255)
                                                                    ccAGAGCAACAACATTTGTGGCATTACGACGGAGCACCCGCCGCACCGACATCCACCGGTTAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTc                                                                                   <  2:3080422/150‑1 (MQ=255)
                                                                    ccAGACAAACAACTTTTGTGTAATAACGCCGGAGCACCAGCCCCACCGACACCCACCGTTCAATTAAACACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTc                                                                                   <  1:4409964/150‑1 (MQ=255)
                                                                      agagCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGc                                                                                 >  2:3362465/1‑150 (MQ=255)
                                                                         gCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGt                                                                              <  2:5065687/150‑1 (MQ=255)
                                                                            acaccCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGGTAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATc                                                                           <  2:2637428/150‑1 (MQ=255)
                                                                              aacCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCt                                                                         >  2:2030044/1‑150 (MQ=255)
                                                                                     gtgGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCg                                                                  <  1:406393/150‑1 (MQ=255)
                                                                                       ggCATTACGACGGAGCACCAGCCGCACCGACATCCCCCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGcc                                                                <  2:2137158/150‑1 (MQ=255)
                                                                                        gCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCa                                                               <  2:3104309/150‑1 (MQ=255)
                                                                                        gCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCa                                                               >  1:179465/1‑150 (MQ=255)
                                                                                         cATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCAt                                                              <  2:3395678/150‑1 (MQ=255)
                                                                                          aTTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATc                                                             >  1:1611785/1‑150 (MQ=255)
                                                                                           ttACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCa                                                            >  1:3418057/1‑150 (MQ=255)
                                                                                           ttACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCa                                                            >  1:3417932/1‑150 (MQ=255)
                                                                                           ttACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCa                                                            <  1:2855644/150‑1 (MQ=255)
                                                                                            tACGACGGAGCACCAGCCGCACCTACATCCACCGGTTAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCaa                                                           <  1:2343662/150‑1 (MQ=255)
                                                                                                 cgGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATacga                                                      >  2:2954964/1‑150 (MQ=255)
                                                                                                    aGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGt                                                   >  1:1853552/1‑150 (MQ=255)
                                                                                                      cACCAGCCGCACCGACATCCACCGGTGAATTCAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGc                                                 <  2:3015724/150‑1 (MQ=255)
                                                                                                      cACCAGCCCCACCGACATCCACCGTTTAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGc                                                 <  1:3143671/150‑1 (MQ=255)
                                                                                                        ccAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGctc                                               >  2:916072/1‑150 (MQ=255)
                                                                                                        ccAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGctc                                               <  1:5063034/150‑1 (MQ=255)
                                                                                                          aGCCGCACCGACATCCACCGGTGAATTAAACACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCtctc                                             <  1:2980559/150‑1 (MQ=255)
                                                                                                            ccGCACCGACATCCCCCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCt                                           <  2:28930/150‑1 (MQ=255)
                                                                                                             cGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTccaa                                          >  1:4691865/1‑148 (MQ=255)
                                                                                                                    aCATCCACCGTTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTa                                   <  2:2724887/150‑1 (MQ=255)
                                                                                                                      aTCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTAtt                                 >  2:2004403/1‑150 (MQ=255)
                                                                                                                       tCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTAttt                                <  2:852549/150‑1 (MQ=255)
                                                                                                                         cACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTg                              <  2:696365/150‑1 (MQ=255)
                                                                                                                           ccGTTGAATAAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGcc                            <  1:594551/150‑1 (MQ=255)
                                                                                                                             ggTGAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGt                          <  1:2240785/150‑1 (MQ=255)
                                                                                                                              ttGAATAAAACACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTc                         <  1:1946482/149‑1 (MQ=255)
                                                                                                                                gAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAg                       >  1:1093704/1‑150 (MQ=255)
                                                                                                                                gAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAg                       >  1:3691704/1‑150 (MQ=255)
                                                                                                                                gAATTAAAAACGCCAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAg                       <  1:4061746/150‑1 (MQ=255)
                                                                                                                                   ttAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCt                    >  2:565029/1‑150 (MQ=255)
                                                                                                                                       aaaCGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACa                >  2:3468872/1‑150 (MQ=255)
                                                                                                                                         aCGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATa              <  2:3716913/150‑1 (MQ=255)
                                                                                                                                         aCGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATa              <  2:1117607/150‑1 (MQ=255)
                                                                                                                                           gCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAgg            <  1:2881014/150‑1 (MQ=255)
                                                                                                                                           gCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAgg            >  1:4224005/1‑150 (MQ=255)
                                                                                                                                             cAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGc          >  2:1504167/1‑150 (MQ=255)
                                                                                                                                             cAGCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGc          <  2:3274461/150‑1 (MQ=255)
                                                                                                                                              aGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCa         >  1:1394486/1‑150 (MQ=255)
                                                                                                                                              aGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCa         >  1:3990475/1‑150 (MQ=255)
                                                                                                                                               gCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCAc        <  1:4491383/150‑1 (MQ=255)
                                                                                                                                               gCTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCAc        <  1:1890250/150‑1 (MQ=255)
                                                                                                                                                cTCACACCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATTCGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCAcc       >  2:3062869/1‑150 (MQ=255)
                                                                                                                                                 tCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCAccc      <  2:3023721/150‑1 (MQ=255)
                                                                                                                                                  cacaCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCGCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCACCCa     <  2:949170/150‑1 (MQ=255)
                                                                                                                                                  cACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTAGCGGGTATTTTGCCGTCAGGCTGACATAGGGCACCCa     >  2:3461116/1‑150 (MQ=255)
                                                                                                                                                   aCCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCACCCAg    <  2:4783280/150‑1 (MQ=255)
                                                                                                                                                    ccccGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCACCCAgg   <  2:723927/150‑1 (MQ=255)
                                                                                                                                                     cccGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCACCCAGGc  >  1:4566124/1‑150 (MQ=255)
                                                                                                                                                     cccGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCACCCAGGc  <  2:2483960/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGGCAAGGGATTGTTCAGCCATAAGATCCTGATTCAGACGCCAGGTTCCCGGTATGAACAGAGCAATCCAGAGCAACAACCTTTGTGGCATTACGACGGAGCACCAGCCGCACCGACATCCACCGGTGAATTAAAAACGCCAGCTCACCCCGGTGACAAAGGTAAAACTCTCATTACGATCAATCATCGGGCTGTCGGTAATCTCTTCCGGCAGCTCGCTGTATCCTGCGCTCGCCATCAATACGACGTGCTCTCCTATCGGGTATTTTGCCGTCAGGCTGACATAGGGCACCCAGGC  >  CP009273/3744357‑3744655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A