Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,830,197 C→A 70.1% intergenic (+281/‑116) yicT → / → setC pseudogene/putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,830,1970CA70.1% 259.8 / 171.3 183intergenic (+281/‑116)yicT/setCpseudogene/putative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base C (29/26);  new base A (73/55);  total (102/81)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

ATTTGTTCATTTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATA  >  CP009273/3830050‑3830345
                                                                                                                                                   |                                                                                                                                                    
aTTTGTTCATTTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTgagc                                                                                                                                                    >  2:178794/1‑150 (MQ=255)
aTTTGTTCATTTAATATATCAAATCAACTTGTTTTATTGTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCGTTTACTAATAATCTCAATTGCTGGCCCTATAATATTgagc                                                                                                                                                    >  2:168569/1‑150 (MQ=255)
 tttGTTCATTTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCt                                                                                                                                                   >  1:199109/1‑150 (MQ=255)
  ttGTTCATTTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTa                                                                                                                                                  >  2:4755807/1‑150 (MQ=255)
  ttGTTCATTTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTa                                                                                                                                                  >  1:2303930/1‑150 (MQ=255)
     ttCATTTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCa                                                                                                                                               <  2:4509650/150‑1 (MQ=255)
         tttAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGc                                                                                                                                           >  1:1597028/1‑150 (MQ=255)
          ttAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCt                                                                                                                                          <  2:790930/150‑1 (MQ=255)
            aaTATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCttt                                                                                                                                        <  1:1488367/150‑1 (MQ=255)
             atatatCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTc                                                                                                                                       <  2:2027069/150‑1 (MQ=255)
                tatCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTAATAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTgg                                                                                                                                    >  2:4881025/1‑150 (MQ=255)
                 atcaaatcaaCTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATTCTTTTACTAATAATCTCAATTGCTGGCCCTCTAATATTGAGCTAGCATTGCTTTCTGGt                                                                                                                                   >  2:3244439/1‑150 (MQ=255)
                 atcaaatcaaCTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGt                                                                                                                                   >  2:638489/1‑150 (MQ=255)
                  tcaaatcaaCTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGtt                                                                                                                                  <  2:1750474/150‑1 (MQ=255)
                   caaatcaaCTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATCATATTGCGCTAGCATTGCTTTCTGGTTg                                                                                                                                 >  2:3390702/1‑150 (MQ=255)
                   caaatcaaCTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTg                                                                                                                                 <  2:1611674/150‑1 (MQ=255)
                   caaatcaaCTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGACCTCTAATATTGAGCTAGCATTGCTTTCTGGTTg                                                                                                                                 >  2:2176615/1‑150 (MQ=255)
                     aatcaaCTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTa                                                                                                                               >  1:2670959/1‑150 (MQ=255)
                     aatcaaCTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTa                                                                                                                               <  1:1064289/150‑1 (MQ=255)
                       tcaaCTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATc                                                                                                                             >  2:1488756/1‑150 (MQ=255)
                        caaCTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCa                                                                                                                            >  2:3197190/1‑150 (MQ=255)
                          aCTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGc                                                                                                                          <  1:649493/150‑1 (MQ=255)
                            ttGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGa                                                                                                                        <  2:4936047/150‑1 (MQ=255)
                             tGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGTTTGTATCAGCGAt                                                                                                                       >  1:3023669/1‑150 (MQ=255)
                               ttttATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATAc                                                                                                                     >  2:4243403/1‑150 (MQ=255)
                                tttATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACt                                                                                                                    <  1:1090836/150‑1 (MQ=255)
                                 ttATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTc                                                                                                                   >  2:2516118/1‑150 (MQ=255)
                                     ttttACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCaaaa                                                                                                               >  1:4236047/1‑150 (MQ=255)
                                        tACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAAtt                                                                                                            <  1:2478731/150‑1 (MQ=255)
                                        tACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAAtt                                                                                                            >  2:1046502/1‑150 (MQ=255)
                                         aCTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTc                                                                                                           <  2:3522198/150‑1 (MQ=255)
                                           tATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCATTCTGGTTGTATCAGCGATACTCAAAAATTCtt                                                                                                         >  1:4796281/1‑150 (MQ=255)
                                             tCAAAAATAGCTCATTGACTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAAAATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTaa                                                                                                       <  2:1002245/150‑1 (MQ=255)
                                              cAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTaat                                                                                                      <  2:1460991/150‑1 (MQ=255)
                                              cAAAAATAGATCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTaat                                                                                                      <  2:4436381/150‑1 (MQ=255)
                                                aaaaTAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTaatac                                                                                                    >  1:1374658/1‑150 (MQ=255)
                                                  aaTAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTaatacaa                                                                                                  >  1:3736016/1‑150 (MQ=255)
                                                   aTAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTaatacaat                                                                                                 >  2:93879/1‑150 (MQ=255)
                                                       cTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTc                                                                                             >  1:3951366/1‑150 (MQ=255)
                                                       cTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTc                                                                                             <  1:185631/150‑1 (MQ=255)
                                                       cTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTc                                                                                             >  1:2395177/1‑150 (MQ=255)
                                                          aTTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCAtt                                                                                          >  1:4222787/1‑150 (MQ=255)
                                                            tGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCg                                                                                        >  1:143157/1‑150 (MQ=255)
                                                               cTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACt                                                                                     >  2:1996875/1‑150 (MQ=255)
                                                                 tACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTgg                                                                                   <  1:1445904/150‑1 (MQ=255)
                                                                 tACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTgg                                                                                   >  1:3690707/1‑150 (MQ=255)
                                                                 tACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTgg                                                                                   >  1:3378350/1‑150 (MQ=255)
                                                                  aCTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGt                                                                                  <  1:5154898/150‑1 (MQ=255)
                                                                    tataATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTAc                                                                                <  2:4700272/150‑1 (MQ=255)
                                                                     ataATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACt                                                                               >  1:2806558/1‑150 (MQ=255)
                                                                      taattaatATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACtt                                                                              <  1:2054768/150‑1 (MQ=255)
                                                                        attaatATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTAt                                                                            >  1:3875401/1‑150 (MQ=255)
                                                                           aatATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTg                                                                         >  1:4136906/1‑150 (MQ=255)
                                                                               tataAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACACTACTCATTCGACTGGTCCTTATTTGTAAc                                                                     >  2:26373/1‑150 (MQ=255)
                                                                                ataAATTCGTTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACt                                                                    <  2:326603/150‑1 (MQ=255)
                                                                                 taAATTCGGTAATTAATTCTTAACATGCTTTTACTAATACTCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTc                                                                   <  2:4917974/150‑1 (MQ=255)
                                                                                 taAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTc                                                                   >  2:593010/1‑150 (MQ=255)
                                                                                 taAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATGGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTc                                                                   <  2:2948785/150‑1 (MQ=255)
                                                                                    aTTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGt                                                                >  1:4887078/1‑150 (MQ=255)
                                                                                          taattaatTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATAtt                                                          >  1:2419704/1‑150 (MQ=255)
                                                                                           aattaattCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATAttt                                                         >  2:3620780/1‑150 (MQ=255)
                                                                                              taattCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATAtttttt                                                      >  1:758719/1‑150 (MQ=255)
                                                                                               aattCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTc                                                     <  1:362784/150‑1 (MQ=255)
                                                                                               aattCTTAACATGATTTTACTAATAATCTCAATTGCTGGCACTATAATATTGCGCTAGCATTGCTTTCTGTTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTc                                                     <  1:363018/150‑1 (MQ=255)
                                                                                                attCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCg                                                    <  2:296754/150‑1 (MQ=255)
                                                                                                attCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCg                                                    <  2:297075/150‑1 (MQ=255)
                                                                                                attCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCg                                                    >  2:3450975/1‑150 (MQ=255)
                                                                                                  tCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGcc                                                  >  1:825398/1‑150 (MQ=255)
                                                                                                       aCATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTg                                             >  1:4852777/1‑150 (MQ=255)
                                                                                                       aCATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTg                                             >  1:2635598/1‑150 (MQ=255)
                                                                                                       aCATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTg                                             <  2:449669/150‑1 (MQ=255)
                                                                                                         aTGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGAt                                           >  2:5055078/1‑150 (MQ=255)
                                                                                                         aTGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTAGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGGCCGGTGAt                                           >  2:3175039/1‑150 (MQ=255)
                                                                                                          tNCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGAtt                                          >  1:2557420/1‑150 (MQ=255)
                                                                                                             ttttACTTATAATCTCAATTGCTGGCCCTATAATCTTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCag                                       <  1:2975206/150‑1 (MQ=255)
                                                                                                             ttttACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCag                                       <  1:1664120/150‑1 (MQ=255)
                                                                                                             ttttACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCag                                       <  1:1224766/150‑1 (MQ=255)
                                                                                                                tactacTAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCagaat                                    <  2:2068913/150‑2 (MQ=255)
                                                                                                                tACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCagaag                                    <  2:3738991/150‑1 (MQ=255)
                                                                                                                        atCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCaaa                            >  1:3716580/1‑150 (MQ=255)
                                                                                                                           tcAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCaaaaaa                         <  2:859999/150‑1 (MQ=255)
                                                                                                                            cAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAAc                        >  1:3714932/1‑150 (MQ=255)
                                                                                                                            cAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAAc                        <  2:1948245/150‑1 (MQ=255)
                                                                                                                            cAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAAc                        >  2:2432130/1‑150 (MQ=255)
                                                                                                                              aTTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACgg                      >  2:3977015/1‑150 (MQ=255)
                                                                                                                              aTTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACgg                      >  2:3976601/1‑150 (MQ=255)
                                                                                                                                tGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATCATGCCAAAAACGGCt                    >  2:4938864/1‑150 (MQ=255)
                                                                                                                                tGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCAGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCt                    >  2:3071746/1‑150 (MQ=255)
                                                                                                                                tGCTGGCCATATATTTTTGCGTTTGAATTGTTTTCTGGTTGTATCTGAGATACTAAAAAATTCTTAATACAATACTCTTTCGACTGGTACTTATTTTTAACTCAGTTTTTTTTTTTCGCCCTTTTTTTCAGCATAATGCAAAAAACGGCt                    <  1:2373533/150‑1 (MQ=255)
                                                                                                                                 gCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTa                   <  1:4372787/150‑1 (MQ=255)
                                                                                                                                  cTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTAc                  <  2:4324867/150‑1 (MQ=255)
                                                                                                                                   tGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTAcc                 >  2:4946976/1‑150 (MQ=255)
                                                                                                                                   tGGCCCTATAATATTGAGCTAGCATAGCTTTCGGGTTGTATCAGCGATACTCAAAAATTCTTAATACACTACTCATTCGACTGGTACTTATTTGTAAATCAGTTATATTTTTTCGCCCGGTGATTCTGAATAATGCAAAAAAAGGCTAcc                 >  2:1211863/1‑150 (MQ=255)
                                                                                                                                     gCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCAc               <  1:4931069/150‑1 (MQ=255)
                                                                                                                                        cTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTcc            >  2:691089/1‑150 (MQ=255)
                                                                                                                                          ataataTTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATAATCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCAt          >  1:4728611/1‑150 (MQ=255)
                                                                                                                                           taataTTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTCCCACTCCATc         >  2:4380124/1‑150 (MQ=255)
                                                                                                                                            aataTTGCGCTAGCATTGGTTTGTTGTTTTATCTGCGATACTCAAAAATTCTTAATACAATACTCATTCTACTTTTACTTATTTTTAACTAAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGAAAAAAACGGCTACCACTCCATCa        <  1:3644882/150‑1 (MQ=255)
                                                                                                                                             atatTGCGCTAGCATTGCTTTCTGGTTGTATCAGCTATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGAAAAAAACGGCTACCACTCCATCaa       <  1:1062615/150‑1 (MQ=255)
                                                                                                                                                  gcgcTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATa  <  2:2917729/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATTTGTTCATTTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATA  >  CP009273/3830050‑3830345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTGTTCATTTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATA  >  CP009273/3830051‑3830345
                                                                                                                                                  |                                                                                                                                                    
TTTGTTCATTTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCT                                                                                                                                                   >  SRR19973083.200224/1‑150 (MQ=60)
 TTGTTCATTTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTA                                                                                                                                                  >  SRR19973083.2315737/1‑150 (MQ=60)
        TTTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGC                                                                                                                                           >  SRR19973083.1605021/1‑150 (MQ=60)
         TTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCT                                                                                                                                          <  SRR19973083.1808186/150‑1 (MQ=60)
           AATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTT                                                                                                                                        <  SRR19973083.1495866/150‑1 (MQ=60)
            ATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTC                                                                                                                                       >  SRR19973083.1958205/1‑150 (MQ=60)
                 TCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTT                                                                                                                                  >  SRR19973083.2592237/1‑150 (MQ=60)
                    AATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTA                                                                                                                               >  SRR19973083.2684367/1‑150 (MQ=60)
                    AATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTA                                                                                                                               >  SRR19973083.2684365/1‑150 (MQ=60)
                    AATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTA                                                                                                                               <  SRR19973083.1069649/150‑1 (MQ=60)
                    AATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTA                                                                                                                               >  SRR19973083.5043330/1‑150 (MQ=60)
                    AATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTA                                                                                                                               <  SRR19973083.1069770/150‑1 (MQ=60)
                     ATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTAT                                                                                                                              >  SRR19973083.1434154/1‑150 (MQ=60)
                      TCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATC                                                                                                                             <  SRR19973083.1236712/150‑1 (MQ=60)
                         ACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGC                                                                                                                          <  SRR19973083.652758/150‑1 (MQ=60)
                           TTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGA                                                                                                                        >  SRR19973083.2954001/1‑150 (MQ=60)
                            TGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGTTTGTATCAGCGAT                                                                                                                       >  SRR19973083.3038223/1‑150 (MQ=60)
                            TGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGAT                                                                                                                       >  SRR19973083.4869465/1‑150 (MQ=60)
                            TGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGAT                                                                                                                       <  SRR19973083.3541504/150‑1 (MQ=60)
                             GTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATA                                                                                                                      >  SRR19973083.3033651/1‑150 (MQ=60)
                               TTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACT                                                                                                                    <  SRR19973083.1096330/150‑1 (MQ=60)
                                   TTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAA                                                                                                                >  SRR19973083.3752682/1‑150 (MQ=60)
                                    TTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAA                                                                                                               >  SRR19973083.3702716/1‑150 (MQ=60)
                                    TTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAA                                                                                                               >  SRR19973083.4254594/1‑150 (MQ=60)
                                       TACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATT                                                                                                            <  SRR19973083.2491408/150‑1 (MQ=60)
                                          TATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCATTCTGGTTGTATCAGCGATACTCAAAAATTCTT                                                                                                         >  SRR19973083.4816794/1‑150 (MQ=60)
                                              AAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATA                                                                                                     >  SRR19973083.1477708/1‑150 (MQ=60)
                                               AAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATAC                                                                                                    >  SRR19973083.1381478/1‑150 (MQ=60)
                                                 AATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAA                                                                                                  >  SRR19973083.3752970/1‑150 (MQ=60)
                                                      CTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTC                                                                                             >  SRR19973083.2407491/1‑150 (MQ=60)
                                                      CTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTC                                                                                             >  SRR19973083.3968950/1‑150 (MQ=60)
                                                      CTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTC                                                                                             <  SRR19973083.186682/150‑1 (MQ=60)
                                                       TCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCA                                                                                            >  SRR19973083.2265707/1‑150 (MQ=60)
                                                         ATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATT                                                                                          >  SRR19973083.4241304/1‑150 (MQ=60)
                                                         ATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATT                                                                                          >  SRR19973083.4889853/1‑150 (MQ=60)
                                                           TGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCG                                                                                        <  SRR19973083.2365365/150‑1 (MQ=60)
                                                           TGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCG                                                                                        >  SRR19973083.144033/1‑150 (MQ=60)
                                                              CTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACT                                                                                     >  SRR19973083.655991/1‑150 (MQ=60)
                                                                TACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGG                                                                                   >  SRR19973083.3707673/1‑150 (MQ=60)
                                                                TACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGG                                                                                   >  SRR19973083.777107/1‑150 (MQ=60)
                                                                TACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGG                                                                                   >  SRR19973083.3707531/1‑150 (MQ=60)
                                                                TACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGG                                                                                   >  SRR19973083.3394023/1‑150 (MQ=60)
                                                                TACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGG                                                                                   <  SRR19973083.1453171/150‑1 (MQ=60)
                                                                 ACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGT                                                                                  <  SRR19973083.5176782/150‑1 (MQ=60)
                                                                 ACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGT                                                                                  >  SRR19973083.4765609/1‑150 (MQ=60)
                                                                  CTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTA                                                                                 >  SRR19973083.1445161/1‑150 (MQ=60)
                                                                   TATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTAC                                                                                <  SRR19973083.613001/150‑1 (MQ=60)
                                                                    ATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACT                                                                               >  SRR19973083.2820478/1‑150 (MQ=60)
                                                                     TAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTT                                                                              <  SRR19973083.2065241/150‑1 (MQ=60)
                                                                       ATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTAT                                                                            >  SRR19973083.3892861/1‑150 (MQ=60)
                                                                       ATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTAT                                                                            >  SRR19973083.3892707/1‑150 (MQ=60)
                                                                          AATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTG                                                                         >  SRR19973083.4155187/1‑150 (MQ=60)
                                                                          AATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTG                                                                         >  SRR19973083.4155567/1‑150 (MQ=60)
                                                                          AATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTG                                                                         >  SRR19973083.4311441/1‑150 (MQ=60)
                                                                                   ATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGT                                                                >  SRR19973083.4908076/1‑150 (MQ=60)
                                                                                   ATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGT                                                                >  SRR19973083.2535908/1‑150 (MQ=60)
                                                                                         TAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATT                                                          >  SRR19973083.2432131/1‑150 (MQ=60)
                                                                                             TAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTT                                                      >  SRR19973083.762429/1‑150 (MQ=60)
                                                                                              AATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTC                                                     <  SRR19973083.364499/150‑1 (MQ=60)
                                                                                              AATTCTTAACATGATTTTACTAATAATCTCAATTGCTGGCACTATAATATTGCGCTAGCATTGCTTTCTGTTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTC                                                     <  SRR19973083.364733/150‑1 (MQ=60)
                                                                                                 TCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCC                                                  >  SRR19973083.829321/1‑150 (MQ=60)
                                                                                                    TAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGG                                               >  SRR19973083.1601830/1‑150 (MQ=60)
                                                                                                    TAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGG                                               >  SRR19973083.383004/1‑150 (MQ=60)
                                                                                                      ACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTG                                             >  SRR19973083.4873611/1‑150 (MQ=60)
                                                                                                      ACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTG                                             <  SRR19973083.2029376/150‑1 (MQ=60)
                                                                                                      ACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTG                                             >  SRR19973083.2648840/1‑150 (MQ=60)
                                                                                                      ACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTG                                             >  SRR19973083.2648853/1‑150 (MQ=60)
                                                                                                         TNCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATT                                          >  SRR19973083.2570355/1‑150 (MQ=60)
                                                                                                           CTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCA                                        <  SRR19973083.4437946/150‑1 (MQ=60)
                                                                                                            TTTTACTTATAATCTCAATTGCTGGCCCTATAATCTTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAG                                       <  SRR19973083.2989641/150‑1 (MQ=60)
                                                                                                            TTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAG                                       <  SRR19973083.1230783/150‑1 (MQ=60)
                                                                                                            TTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAG                                       <  SRR19973083.1672371/150‑1 (MQ=60)
                                                                                                            TTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAG                                       <  SRR19973083.1230677/150‑1 (MQ=60)
                                                                                                                ACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATA                                   <  SRR19973083.4244037/150‑1 (MQ=60)
                                                                                                                       ATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAA                            >  SRR19973083.3733483/1‑150 (MQ=60)
                                                                                                                       ATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAA                            >  SRR19973083.3733374/1‑150 (MQ=60)
                                                                                                                           CAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAAC                        >  SRR19973083.3731831/1‑150 (MQ=60)
                                                                                                                               TGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCT                    <  SRR19973083.2420793/150‑1 (MQ=60)
                                                                                                                               TGCTGGCCATATATTTTTGCGTTTGAATTGTTTTCTGGTTGTATCTGAGATACTAAAAAATTCTTAATACAATACTCTTTCGACTGGTACTTATTTTTAACTCAGTTTTTTTTTTTCGCCCTTTTTTTCAGCATAATGCAAAAAACGGCT                    <  SRR19973083.2385749/150‑1 (MQ=45)
                                                                                                                                GCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTA                   <  SRR19973083.4391784/150‑1 (MQ=60)
                                                                                                                                    GCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCAC               <  SRR19973083.4952275/150‑1 (MQ=60)
                                                                                                                                         ATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCAT          <  SRR19973083.3221927/150‑1 (MQ=60)
                                                                                                                                         ATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATAATCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCAT          >  SRR19973083.4748824/1‑150 (MQ=60)
                                                                                                                                           AATATTGCGCTAGCATTGGTTTGTTGTTTTATCTGCGATACTCAAAAATTCTTAATACAATACTCATTCTACTTTTACTTATTTTTAACTAAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGAAAAAAACGGCTACCACTCCATCA        <  SRR19973083.3661558/150‑1 (MQ=60)
                                                                                                                                           AATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTAATTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCA        <  SRR19973083.3661254/150‑1 (MQ=60)
                                                                                                                                           AATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCA        >  SRR19973083.2400017/1‑150 (MQ=60)
                                                                                                                                            ATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCTATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGAAAAAAACGGCTACCACTCCATCAA       <  SRR19973083.1067968/150‑1 (MQ=60)
                                                                                                                                                TGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAAT   <  SRR19973083.3212388/150‑1 (MQ=60)
                                                                                                                                                TGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAAT   >  SRR19973083.2633569/1‑150 (MQ=60)
                                                                                                                                                TGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTAATTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCATAATATTGCAAAAAACGGCTACCACTCCATCAAAAAT   <  SRR19973083.94517/150‑1 (MQ=60)
                                                                                                                                                 GAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATA  >  SRR19973083.6631/1‑150 (MQ=60)
                                                                                                                                                 GAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATA  >  SRR19973083.711947/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
TTTGTTCATTTAATATATCAAATCAACTTGTTTTATTTTTACTATCAAAAATAGCTCATTGCCTTACTATAATTAATATATAAATTCGGTAATTAATTCTTAACATGCTTTTACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATA  >  CP009273/3830051‑3830345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑