Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,000,841 G→T 88.5% A206A (GCG→GCT)  pyrD → dihydro‑orotate oxidase, FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,000,8410GT88.5% 404.3 / 46.5 163A206A (GCG→GCT) pyrDdihydro‑orotate oxidase, FMN‑linked
Reads supporting (aligned to +/- strand):  ref base G (8/11);  new base T (70/74);  total (78/85)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

GTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTT  >  CP009273/1000696‑1000985
                                                                                                                                                 |                                                                                                                                                
 tATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATg                                                                                                                                               <  1:39394/148‑1 (MQ=255)
 tATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATg                                                                                                                                               <  1:39275/148‑1 (MQ=255)
 tATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATg                                                                                                                                               <  1:1716770/148‑1 (MQ=255)
  aTGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGc                                                                                                                                              >  2:2297422/1‑148 (MQ=255)
  aTGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGc                                                                                                                                              >  2:2543083/1‑148 (MQ=255)
   tGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCa                                                                                                                                             >  2:782116/1‑148 (MQ=255)
   tGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCa                                                                                                                                             >  2:2469567/1‑148 (MQ=255)
    ggAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAc                                                                                                                                            <  2:1396605/148‑1 (MQ=255)
    ggAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAc                                                                                                                                            >  1:2420202/1‑148 (MQ=255)
    ggAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAc                                                                                                                                            >  1:761356/1‑148 (MQ=255)
         aaaTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATaa                                                                                                                                       <  1:1175914/148‑1 (MQ=255)
          aaTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATaaa                                                                                                                                      >  2:1119646/1‑148 (MQ=255)
           aTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAat                                                                                                                                     >  2:499909/1‑148 (MQ=255)
           aTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAat                                                                                                                                     <  2:916123/148‑1 (MQ=255)
            tCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAata                                                                                                                                    <  2:1315709/148‑1 (MQ=255)
             ctatgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAatat                                                                                                                                   <  1:346645/148‑1 (MQ=255)
               atgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtgt                                                                                                                                 >  1:2866025/1‑148 (MQ=255)
               atgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtgt                                                                                                                                 >  1:619131/1‑148 (MQ=255)
                tgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtgtg                                                                                                                                >  1:1147239/1‑148 (MQ=255)
                 gcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGc                                                                                                                               >  1:3033702/1‑148 (MQ=255)
                   ctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCg                                                                                                                             >  1:1992535/1‑148 (MQ=255)
                   ctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCg                                                                                                                             >  1:1992162/1‑148 (MQ=255)
                     atgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGAt                                                                                                                           <  1:2544847/148‑1 (MQ=255)
                           gATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGt                                                                                                                     <  1:2589728/148‑1 (MQ=255)
                             tatatCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGa                                                                                                                   <  1:3298066/148‑1 (MQ=255)
                              atatCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGaa                                                                                                                  >  2:363142/1‑148 (MQ=255)
                                atCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGa                                                                                                                >  1:464298/1‑148 (MQ=255)
                                atCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGa                                                                                                                <  2:1899509/148‑1 (MQ=255)
                                atCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGa                                                                                                                <  2:2002187/148‑1 (MQ=255)
                                  cGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAATATc                                                                                                              >  2:650730/1‑148 (MQ=255)
                                   gCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATcg                                                                                                             <  1:3162709/148‑1 (MQ=255)
                                   gCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATcg                                                                                                             <  1:2083252/148‑1 (MQ=255)
                                     cATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATcgcg                                                                                                           >  2:173598/1‑148 (MQ=255)
                                       tCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGcc                                                                                                         >  2:378702/1‑148 (MQ=255)
                                            atTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATc                                                                                                    <  1:1580216/148‑1 (MQ=255)
                                            acttCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATc                                                                                                    <  2:1220895/146‑1 (MQ=255)
                                                       gAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaa                                                                                         <  2:259095/148‑1 (MQ=255)
                                                         aTACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaaga                                                                                       <  2:2049120/148‑1 (MQ=255)
                                                           aCCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAAt                                                                                     >  1:1857785/1‑148 (MQ=255)
                                                            ccccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAAtt                                                                                    <  1:2010097/148‑1 (MQ=255)
                                                             cccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTg                                                                                   <  2:2112778/148‑1 (MQ=255)
                                                               cAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGAt                                                                                 >  2:998087/1‑148 (MQ=255)
                                                                aGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATc                                                                                <  2:492963/148‑1 (MQ=255)
                                                                  gATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGTATTGATCCa                                                                              >  2:2314407/1‑148 (MQ=255)
                                                                  gATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCa                                                                              >  2:652096/1‑148 (MQ=255)
                                                                     tACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGt                                                                           >  1:928445/1‑148 (MQ=255)
                                                                     tACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGt                                                                           <  1:2317998/148‑1 (MQ=255)
                                                                       cgcacgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTg                                                                         <  2:414598/148‑1 (MQ=255)
                                                                       cgcacgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTg                                                                         <  1:2155261/148‑1 (MQ=255)
                                                                       cgcacgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTg                                                                         <  1:2279352/148‑1 (MQ=255)
                                                                        gcacgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGc                                                                        <  1:1004541/148‑1 (MQ=255)
                                                                            gcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAt                                                                    <  2:756578/148‑1 (MQ=255)
                                                                            gcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAt                                                                    <  2:2761222/148‑1 (MQ=255)
                                                                                   tatGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttag                                                             >  1:3019882/1‑148 (MQ=255)
                                                                                    atGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttagt                                                            <  1:2912581/148‑1 (MQ=255)
                                                                                        tGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGc                                                        <  1:1441651/148‑1 (MQ=255)
                                                                                         gAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGcc                                                       >  1:361709/1‑148 (MQ=255)
                                                                                         gAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGcc                                                       >  1:1427308/1‑148 (MQ=255)
                                                                                           aGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCat                                                     >  1:2390287/1‑148 (MQ=255)
                                                                                           aGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATTCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCat                                                     <  1:2281811/148‑1 (MQ=255)
                                                                                             cgcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataa                                                   >  2:405824/1‑148 (MQ=255)
                                                                                             cgcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataa                                                   >  2:2836254/1‑148 (MQ=255)
                                                                                             cgcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGCTGCCGATAGTTTAGTTCGCCataa                                                   <  1:2300025/148‑1 (MQ=255)
                                                                                              gcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataat                                                  <  2:1117988/148‑1 (MQ=255)
                                                                                                 ggATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATAtt                                               <  1:574089/148‑1 (MQ=255)
                                                                                                 ggATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATAtt                                               >  1:3273989/1‑148 (MQ=255)
                                                                                                  gatgatCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAGAGTTTAGTTCGCCATAATATTg                                              >  2:1233825/1‑148 (MQ=255)
                                                                                                     gatCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATg                                           >  2:2949567/1‑148 (MQ=255)
                                                                                                      atCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATgg                                          <  2:1673288/148‑1 (MQ=255)
                                                                                                      atCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATgg                                          >  1:10662/1‑148 (MQ=255)
                                                                                                        ctctTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCg                                        >  2:1960065/1‑148 (MQ=255)
                                                                                                          ctTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGtt                                      >  2:3230207/1‑148 (MQ=255)
                                                                                                              aCCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTg                                  <  2:1992535/148‑1 (MQ=255)
                                                                                                              aCCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTg                                  <  2:549456/148‑1 (MQ=255)
                                                                                                                  cgATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAAc                              >  1:2411466/1‑148 (MQ=255)
                                                                                                                    aTTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCAGAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCa                            <  2:2268306/148‑1 (MQ=255)
                                                                                                                     ttAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCaa                           >  2:503258/1‑148 (MQ=255)
                                                                                                                       aaaaaTAAGCAAAATGATGTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATa                         <  2:2960399/148‑1 (MQ=255)
                                                                                                                         ataTAATCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAcc                       <  2:2693578/146‑1 (MQ=255)
                                                                                                                         aaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAcc                       <  2:928445/148‑1 (MQ=255)
                                                                                                                         aaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAcc                       <  2:2733674/148‑1 (MQ=255)
                                                                                                                         aaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAcc                       >  1:31518/1‑148 (MQ=255)
                                                                                                                          aaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACca                      >  1:297126/1‑148 (MQ=255)
                                                                                                                          aaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACca                      <  2:1981245/148‑1 (MQ=255)
                                                                                                                          aaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACca                      >  2:2705419/1‑148 (MQ=255)
                                                                                                                            tAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACcaca                    >  1:2448919/1‑148 (MQ=255)
                                                                                                                            tAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACcaca                    <  1:2082460/148‑1 (MQ=255)
                                                                                                                                 aaaaTGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGa               <  1:2543083/148‑1 (MQ=255)
                                                                                                                                  aaaTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCTATACCACACTCGag              >  2:520941/1‑147 (MQ=255)
                                                                                                                                  aaaTGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGAt              >  1:3149524/1‑148 (MQ=255)
                                                                                                                                   aaTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATc             >  2:1539054/1‑148 (MQ=255)
                                                                                                                                    aTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCg            >  2:1767019/1‑148 (MQ=255)
                                                                                                                                    aTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTGCGCCATAATATTGTTGGCGTTATTGCAACCAATACCACTCTCGATCg            >  2:3080750/1‑148 (MQ=255)
                                                                                                                                     tGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGt           <  1:67339/148‑1 (MQ=255)
                                                                                                                                     tGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGt           <  2:1850063/148‑1 (MQ=255)
                                                                                                                                         ttGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtctc       <  2:3240189/148‑1 (MQ=255)
                                                                                                                                         ttGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtctc       <  1:1119646/148‑1 (MQ=255)
                                                                                                                                         ttGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtctc       <  2:3033702/148‑1 (MQ=255)
                                                                                                                                          tGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTctct      <  2:2989276/148‑1 (MQ=255)
                                                                                                                                            cAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTg    <  2:10662/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTT  >  CP009273/1000696‑1000985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGG  >  CP009273/1000692‑1000990
                                                                                                                                                     |                                                                                                                                                     
ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCT                                                                                                                                                       >  SRR19973070.2939297/1‑150 (MQ=60)
ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCT                                                                                                                                                       >  SRR19973070.2092485/1‑150 (MQ=60)
ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCT                                                                                                                                                       <  SRR19973070.163928/150‑1 (MQ=60)
ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCT                                                                                                                                                       <  SRR19973070.163924/150‑1 (MQ=60)
  TTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTAT                                                                                                                                                     <  SRR19973070.2410745/150‑1 (MQ=60)
  TTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTAT                                                                                                                                                     <  SRR19973070.2410543/150‑1 (MQ=60)
     TATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCA                                                                                                                                                  <  SRR19973070.39853/150‑1 (MQ=60)
     TATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCA                                                                                                                                                  <  SRR19973070.39898/150‑1 (MQ=60)
     TATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCA                                                                                                                                                  <  SRR19973070.39777/150‑1 (MQ=60)
     TATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCA                                                                                                                                                  <  SRR19973070.1728638/150‑1 (MQ=60)
      ATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAC                                                                                                                                                 >  SRR19973070.766707/1‑150 (MQ=60)
      ATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAC                                                                                                                                                 >  SRR19973070.2436120/1‑150 (MQ=60)
             AAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAAT                                                                                                                                          <  SRR19973070.1184154/150‑1 (MQ=60)
                 CTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGT                                                                                                                                      >  SRR19973070.623482/1‑150 (MQ=60)
                 CTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGT                                                                                                                                      <  SRR19973070.349168/150‑1 (MQ=60)
                 CTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGT                                                                                                                                      >  SRR19973070.2884593/1‑150 (MQ=60)
                  TATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTG                                                                                                                                     >  SRR19973070.1155476/1‑150 (MQ=60)
                  TATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTG                                                                                                                                     >  SRR19973070.1155221/1‑150 (MQ=60)
                   ATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGC                                                                                                                                    >  SRR19973070.3053000/1‑150 (MQ=60)
                     GCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCG                                                                                                                                  >  SRR19973070.2005648/1‑150 (MQ=60)
                     GCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCG                                                                                                                                  >  SRR19973070.2006024/1‑150 (MQ=60)
                         ATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCG                                                                                                                              <  SRR19973070.2561482/150‑1 (MQ=60)
                               GATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGA                                                                                                                        >  SRR19973070.2208894/1‑150 (MQ=60)
                               GATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGA                                                                                                                        <  SRR19973070.2606651/150‑1 (MQ=60)
                                ATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTNGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTNAA                                                                                                                       <  SRR19973070.2103492/150‑1 (MQ=60)
                                 TATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAG                                                                                                                      <  SRR19973070.3319197/150‑1 (MQ=60)
                                 TATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAG                                                                                                                      <  SRR19973070.3319342/150‑1 (MQ=60)
                                  ATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTCAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGA                                                                                                                     >  SRR19973070.3096180/1‑150 (MQ=60)
                                  ATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGA                                                                                                                     >  SRR19973070.467611/1‑150 (MQ=60)
                                       GCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCG                                                                                                                <  SRR19973070.3183175/150‑1 (MQ=60)
                                       GCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCG                                                                                                                <  SRR19973070.3182304/150‑1 (MQ=60)
                                       GCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCG                                                                                                                <  SRR19973070.2097268/150‑1 (MQ=60)
                                         CATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCC                                                                                                              >  SRR19973070.1641670/1‑150 (MQ=60)
                                                ATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTT                                                                                                       <  SRR19973070.1591298/150‑1 (MQ=60)
                                                ATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTT                                                                                                       <  SRR19973070.2052587/150‑1 (MQ=60)
                                                   TCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCT                                                                                                    <  SRR19973070.1104084/150‑1 (MQ=60)
                                                     ATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGA                                                                                                  <  SRR19973070.2016824/150‑1 (MQ=60)
                                                     ATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGA                                                                                                  <  SRR19973070.1949453/150‑1 (MQ=60)
                                                            AATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAA                                                                                           >  SRR19973070.765950/1‑150 (MQ=60)
                                                             ATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAAT                                                                                          >  SRR19973070.1870502/1‑150 (MQ=60)
                                                                CCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGA                                                                                       <  SRR19973070.2023705/150‑1 (MQ=60)
                                                                   CAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCC                                                                                    <  SRR19973070.1403407/150‑1 (MQ=60)
                                                                       ATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGT                                                                                >  SRR19973070.935072/1‑150 (MQ=60)
                                                                       ATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGT                                                                                >  SRR19973070.935069/1‑150 (MQ=60)
                                                                         TACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTG                                                                              <  SRR19973070.2333401/150‑1 (MQ=60)
                                                                           CGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCC                                                                            <  SRR19973070.2294495/150‑1 (MQ=60)
                                                                           CGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCC                                                                            <  SRR19973070.2169597/150‑1 (MQ=60)
                                                                            GCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCG                                                                           <  SRR19973070.1011698/150‑1 (MQ=60)
                                                                                    CAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTA                                                                   >  SRR19973070.1542226/1‑150 (MQ=60)
                                                                                     AATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAG                                                                  >  SRR19973070.3039131/1‑150 (MQ=60)
                                                                                        ATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTC                                                               >  SRR19973070.284598/1‑150 (MQ=60)
                                                                                        ATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTC                                                               <  SRR19973070.2931406/150‑1 (MQ=60)
                                                                                           GTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCC                                                            >  SRR19973070.607013/1‑150 (MQ=60)
                                                                                           GTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCC                                                            >  SRR19973070.364357/1‑150 (MQ=60)
                                                                                           GTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCC                                                            >  SRR19973070.1437153/1‑150 (MQ=60)
                                                                                            TGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCA                                                           <  SRR19973070.1451651/150‑1 (MQ=60)
                                                                                             GAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCAT                                                          >  SRR19973070.2406068/1‑150 (MQ=60)
                                                                                               AGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAA                                                        >  SRR19973070.553109/1‑150 (MQ=60)
                                                                                               AGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATTCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAA                                                        <  SRR19973070.2296977/150‑1 (MQ=60)
                                                                                                 CGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGCTGCCGATAGTTTAGTTCGCCATAATA                                                      <  SRR19973070.2315326/150‑1 (MQ=60)
                                                                                                  GCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATAT                                                     <  SRR19973070.248726/150‑1 (MQ=60)
                                                                                                   CTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATT                                                    >  SRR19973070.3295036/1‑150 (MQ=60)
                                                                                                     GGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGA                                                  <  SRR19973070.578012/150‑1 (MQ=60)
                                                                                                       ATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATG                                                >  SRR19973070.3045430/1‑150 (MQ=60)
                                                                                                        TGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGG                                               >  SRR19973070.10831/1‑150 (MQ=60)
                                                                                                         GATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGC                                              <  SRR19973070.381479/150‑1 (MQ=60)
                                                                                                            CTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTT                                           <  SRR19973070.2988953/150‑1 (MQ=60)
                                                                                                                TAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTG                                       >  SRR19973070.259123/1‑150 (MQ=60)
                                                                                                                  ACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCA                                     <  SRR19973070.2312708/150‑1 (MQ=60)
                                                                                                                    CGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAAC                                   >  SRR19973070.2427352/1‑150 (MQ=60)
                                                                                                                    CGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAAC                                   >  SRR19973070.2426503/1‑150 (MQ=60)
                                                                                                                           AAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACC                            >  SRR19973070.31928/1‑150 (MQ=60)
                                                                                                                            AAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCA                           >  SRR19973070.299181/1‑150 (MQ=60)
                                                                                                                              AATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACA                         >  SRR19973070.2464938/1‑150 (MQ=60)
                                                                                                                                TAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACT                       <  SRR19973070.2096468/150‑1 (MQ=60)
                                                                                                                                    CAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGAT                   >  SRR19973070.3169875/1‑150 (MQ=60)
                                                                                                                                     AAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATC                  <  SRR19973070.2559712/150‑1 (MQ=60)
                                                                                                                                         TGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTC              <  SRR19973070.68083/150‑1 (MQ=60)
                                                                                                                                            TTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCT           <  SRR19973070.2397128/150‑1 (MQ=60)
                                                                                                                                             TTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTT          <  SRR19973070.1127298/150‑1 (MQ=60)
                                                                                                                                             TTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTT          <  SRR19973070.2439679/150‑1 (MQ=60)
                                                                                                                                                CAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTT       >  SRR19973070.3244641/1‑150 (MQ=60)
                                                                                                                                                 AAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTC      >  SRR19973070.572239/1‑150 (MQ=60)
                                                                                                                                                 AAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTC      >  SRR19973070.571729/1‑150 (MQ=60)
                                                                                                                                                     TATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGG  <  SRR19973070.174983/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGG  >  CP009273/1000692‑1000990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑