Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,000,841 G→T 100% A206A (GCG→GCT)  pyrD → dihydro‑orotate oxidase, FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,000,8410GT95.8% 644.5 / 17.3 214A206A (GCG→GCT) pyrDdihydro‑orotate oxidase, FMN‑linked
Reads supporting (aligned to +/- strand):  ref base G (1/8);  new base T (102/103);  total (103/111)
Fisher's exact test for biased strand distribution p-value = 3.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

GTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTT  >  CP009273/1000696‑1000985
                                                                                                                                                 |                                                                                                                                                
gTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTAt                                                                                                                                                <  1:2127673/148‑1 (MQ=255)
 tATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATg                                                                                                                                               <  2:2544327/148‑1 (MQ=255)
 tATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATg                                                                                                                                               >  1:79775/1‑148 (MQ=255)
   tGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCa                                                                                                                                             >  2:3045317/1‑148 (MQ=255)
      aaaaaaTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCa                                                                                                                                          <  2:3471133/148‑1 (MQ=255)
           aTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAat                                                                                                                                     >  1:2710224/1‑148 (MQ=255)
           aTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAat                                                                                                                                     <  1:2972660/148‑1 (MQ=255)
           aTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAat                                                                                                                                     >  1:74303/1‑148 (MQ=255)
              tatgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtg                                                                                                                                  >  1:2521789/1‑148 (MQ=255)
                 gcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGc                                                                                                                               <  1:832148/148‑1 (MQ=255)
                   ctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCg                                                                                                                             <  2:1198628/148‑1 (MQ=255)
                      tgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATc                                                                                                                          >  1:1698816/1‑148 (MQ=255)
                        cGGGATATATCGCCATCAATATTTCATCTACGAATAACCCATGATTACGCACGCTGCAATTTGGTTAAGCGATGGATGATATCTTAACCGCGATTAAAAATAAGCAAAATGATTTGAAAGCTATGCACAATAAATATGTGCCGATCGc                                                                                                                        <  1:2162725/148‑1 (MQ=255)
                        cGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGc                                                                                                                        <  1:1486224/148‑1 (MQ=255)
                           gATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGt                                                                                                                     >  1:117953/1‑148 (MQ=255)
                           gATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGt                                                                                                                     >  2:2948317/1‑148 (MQ=255)
                           gATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGt                                                                                                                     >  2:2107360/1‑148 (MQ=255)
                              atatCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGaa                                                                                                                  >  1:2407260/1‑148 (MQ=255)
                               tatCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAg                                                                                                                 <  2:2428814/148‑1 (MQ=255)
                                atCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGa                                                                                                                <  2:2959860/148‑1 (MQ=255)
                                 tCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAACTGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGAt                                                                                                               <  2:3451251/148‑1 (MQ=255)
                                   gCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATcg                                                                                                             <  1:1812060/148‑1 (MQ=255)
                                    ccATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATcgc                                                                                                            >  1:48309/1‑148 (MQ=255)
                                          atatTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGa                                                                                                      >  2:747151/1‑148 (MQ=255)
                                           tatTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGAt                                                                                                     >  2:2905543/1‑148 (MQ=255)
                                             tttCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCt                                                                                                   <  2:458991/148‑1 (MQ=255)
                                                   cGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGTCGATCGCAGTGAAGATCGCGCCGGATCTTTCTga                                                                                             <  1:1644620/148‑1 (MQ=255)
                                                   cGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTga                                                                                             <  1:917129/148‑1 (MQ=255)
                                                      cGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaaga                                                                                          <  1:1107277/148‑1 (MQ=255)
                                                       gAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaa                                                                                         <  1:2220811/148‑1 (MQ=255)
                                                       gAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaa                                                                                         <  1:224220/148‑1 (MQ=255)
                                                         aTACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaaga                                                                                       <  2:1917452/148‑1 (MQ=255)
                                                         aTACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaaga                                                                                       <  1:2274036/148‑1 (MQ=255)
                                                          tACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaagaa                                                                                      <  1:1177953/148‑1 (MQ=255)
                                                            ccccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAAtt                                                                                    >  2:2451779/1‑148 (MQ=255)
                                                            ccccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAAtt                                                                                    <  1:1469922/148‑1 (MQ=255)
                                                             cccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTg                                                                                   <  2:2022339/148‑1 (MQ=255)
                                                             cccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTg                                                                                   <  1:952242/148‑1 (MQ=255)
                                                              ccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGa                                                                                  <  1:1542222/148‑1 (MQ=255)
                                                               cAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGAt                                                                                 >  1:3444960/1‑148 (MQ=255)
                                                                aGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATc                                                                                <  1:3251030/148‑1 (MQ=255)
                                                                aGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATc                                                                                <  1:1744081/148‑1 (MQ=255)
                                                                  gATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTNCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCa                                                                              >  1:1783395/1‑148 (MQ=255)
                                                                  gATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCa                                                                              >  1:3008/1‑148 (MQ=255)
                                                                   aTTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAg                                                                             <  1:1951946/148‑1 (MQ=255)
                                                                   aTTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAg                                                                             <  1:1951701/148‑1 (MQ=255)
                                                                    ttACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAgg                                                                            <  2:3238407/148‑1 (MQ=255)
                                                                     tACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGt                                                                           <  2:2258354/148‑1 (MQ=255)
                                                                       cgcacgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTg                                                                         >  1:2268587/1‑148 (MQ=255)
                                                                       cgcacgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTg                                                                         <  2:2407260/148‑1 (MQ=255)
                                                                             cTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAta                                                                   >  2:3385358/1‑148 (MQ=255)
                                                                               gCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagt                                                                 <  2:1077701/148‑1 (MQ=255)
                                                                               gCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagt                                                                 >  2:950335/1‑148 (MQ=255)
                                                                                cAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtt                                                                <  2:122619/148‑1 (MQ=255)
                                                                                  atatGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagttta                                                              >  1:1562255/1‑148 (MQ=255)
                                                                                  atatGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagttta                                                              <  2:121082/148‑1 (MQ=255)
                                                                                   tatGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttag                                                             <  1:99353/148‑1 (MQ=255)
                                                                                   tatGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttag                                                             <  1:2435856/148‑1 (MQ=255)
                                                                                     tGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttagtt                                                           <  1:1508411/148‑1 (MQ=255)
                                                                                        tGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGc                                                        <  2:2247759/148‑1 (MQ=255)
                                                                                         gAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGcc                                                       <  1:1267870/148‑1 (MQ=255)
                                                                                          aaGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCa                                                      >  1:1234063/1‑148 (MQ=255)
                                                                                            gcgcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCata                                                    <  2:824675/148‑1 (MQ=255)
                                                                                            gcgcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCata                                                    >  2:2463523/1‑148 (MQ=255)
                                                                                            gcgcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCata                                                    <  1:441489/148‑1 (MQ=255)
                                                                                                 ggATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATAtt                                               <  2:926767/148‑1 (MQ=255)
                                                                                                  gatgatCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTg                                              >  1:203723/1‑148 (MQ=255)
                                                                                                  gatgatCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTg                                              >  1:202818/1‑148 (MQ=255)
                                                                                                    tgatCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGAt                                            <  2:15523/148‑1 (MQ=255)
                                                                                                    tgatCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGAt                                            >  2:3156688/1‑148 (MQ=255)
                                                                                                     gatCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATg                                           >  1:2549935/1‑148 (MQ=255)
                                                                                                     gatCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATg                                           <  2:181830/148‑1 (MQ=255)
                                                                                                      atCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATgg                                          >  1:10236/1‑148 (MQ=255)
                                                                                                        ctctTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCg                                        >  1:941234/1‑148 (MQ=255)
                                                                                                        ctctTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCg                                        >  1:1056672/1‑148 (MQ=255)
                                                                                                        ctctTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCg                                        <  2:79775/148‑1 (MQ=255)
                                                                                                          ctTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGtt                                      >  2:2340048/1‑148 (MQ=255)
                                                                                                             aaCCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTAtt                                   >  2:2656006/1‑148 (MQ=255)
                                                                                                             aaCCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTAtt                                   >  2:1599173/1‑148 (MQ=255)
                                                                                                              aCCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTg                                  >  2:2385643/1‑148 (MQ=255)
                                                                                                               ccGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGc                                 >  2:31862/1‑148 (MQ=255)
                                                                                                                cgcgATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCa                                <  2:2605755/148‑1 (MQ=255)
                                                                                                                  cgATGAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATACATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAAc                              <  2:1711883/148‑1 (MQ=255)
                                                                                                                   gATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAAcc                             >  2:2298697/1‑148 (MQ=255)
                                                                                                                     ttAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCaa                           <  2:699975/148‑1 (MQ=255)
                                                                                                                      tAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGCGCCAGGTTGCCGCTTGTTTAGTTCGCCAGAATTTTGATGGCGTTATTGCACCCAAt                          >  2:42924/1‑148 (MQ=255)
                                                                                                                         aaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATaac                       >  2:1053192/1‑146 (MQ=255)
                                                                                                                         aaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAcc                       >  1:674813/1‑148 (MQ=255)
                                                                                                                          aaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACca                      >  1:3192551/1‑148 (MQ=255)
                                                                                                                           aTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACcac                     >  2:2382292/1‑148 (MQ=255)
                                                                                                                               gCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTc                 >  2:3467661/1‑148 (MQ=255)
                                                                                                                                cAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCg                >  2:1050300/1‑148 (MQ=255)
                                                                                                                                 aaaaTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAATAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGa               <  2:1180561/148‑1 (MQ=255)
                                                                                                                                      gATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTAGATCGtt          <  1:1049489/148‑1 (MQ=255)
                                                                                                                                         ttGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtctg       >  2:1882940/1‑147 (MQ=255)
                                                                                                                                         ttGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtctc       >  2:3479705/1‑148 (MQ=255)
                                                                                                                                         ttGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtctc       <  1:724960/148‑1 (MQ=255)
                                                                                                                                         ttGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtctc       <  1:2451779/148‑1 (MQ=255)
                                                                                                                                            cAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTg    <  2:3436790/148‑1 (MQ=255)
                                                                                                                                              aGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGtt  >  1:3371009/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTT  >  CP009273/1000696‑1000985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTC  >  CP009273/1000692‑1000986
                                                                                                                                                     |                                                                                                                                                 
ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCT                                                                                                                                                   >  SRR19973065.2297797/1‑150 (MQ=60)
ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCT                                                                                                                                                   >  SRR19973065.123688/1‑150 (MQ=60)
  TTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTAT                                                                                                                                                 <  SRR19973065.2476984/150‑1 (MQ=60)
   TGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATG                                                                                                                                                >  SRR19973065.80578/1‑150 (MQ=60)
    GTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGC                                                                                                                                               <  SRR19973065.2141366/150‑1 (MQ=60)
      ATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAC                                                                                                                                             <  SRR19973065.2081650/150‑1 (MQ=60)
      ATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAC                                                                                                                                             <  SRR19973065.300803/150‑1 (MQ=60)
             AAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAAT                                                                                                                                      >  SRR19973065.2455950/1‑150 (MQ=60)
             AAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAAT                                                                                                                                      >  SRR19973065.2727074/1‑150 (MQ=60)
             AAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAAT                                                                                                                                      >  SRR19973065.75068/1‑150 (MQ=60)
               ATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAT                                                                                                                                    <  SRR19973065.880547/150‑1 (MQ=60)
               ATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAT                                                                                                                                    <  SRR19973065.2990840/150‑1 (MQ=60)
                TCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATG                                                                                                                                   >  SRR19973065.2537625/1‑150 (MQ=60)
                     GCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCG                                                                                                                              <  SRR19973065.837581/150‑1 (MQ=60)
                      CCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGA                                                                                                                             >  SRR19973065.2284594/1‑150 (MQ=60)
                        TATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATC                                                                                                                           >  SRR19973065.1710145/1‑150 (MQ=60)
                         ATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCG                                                                                                                          >  SRR19973065.932856/1‑150 (MQ=60)
                            CGGGATATATCGCCATCAATATTTCATCTACGAATAACCCATGATTACGCACGCTGCAATTTGGTTAAGCGATGGATGATATCTTAACCGCGATTAAAAATAAGCAAAATGATTTGAAAGCTATGCACAATAAATATGTGCCGATCGCAG                                                                                                                       <  SRR19973065.2176623/150‑1 (MQ=60)
                            CGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAG                                                                                                                       <  SRR19973065.1495710/150‑1 (MQ=60)
                             GGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGT                                                                                                                      >  SRR19973065.118988/1‑150 (MQ=60)
                             GGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGT                                                                                                                      >  SRR19973065.118990/1‑150 (MQ=60)
                              GGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTG                                                                                                                     <  SRR19973065.2494121/150‑1 (MQ=60)
                               GATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGA                                                                                                                    >  SRR19973065.103960/1‑150 (MQ=60)
                                ATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAA                                                                                                                   >  SRR19973065.2422408/1‑150 (MQ=60)
                                  ATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGA                                                                                                                 >  SRR19973065.180947/1‑150 (MQ=60)
                                   TATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGAT                                                                                                                <  SRR19973065.721442/150‑1 (MQ=60)
                                      CGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGC                                                                                                             >  SRR19973065.48860/1‑150 (MQ=60)
                                       GCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCG                                                                                                            >  SRR19973065.737285/1‑150 (MQ=60)
                                       GCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCG                                                                                                            <  SRR19973065.1823983/150‑1 (MQ=60)
                                          ATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCG                                                                                                         >  SRR19973065.1490829/1‑150 (MQ=60)
                                            CAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGA                                                                                                       >  SRR19973065.711621/1‑150 (MQ=60)
                                                  TTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGAACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTC                                                                                                 >  SRR19973065.2429370/1‑150 (MQ=60)
                                                       CGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGTCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAG                                                                                            <  SRR19973065.1655659/150‑1 (MQ=60)
                                                       CGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAG                                                                                            <  SRR19973065.923121/150‑1 (MQ=60)
                                                          CGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAG                                                                                         <  SRR19973065.1114532/150‑1 (MQ=60)
                                                           GAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGA                                                                                        <  SRR19973065.225877/150‑1 (MQ=60)
                                                           GAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGA                                                                                        <  SRR19973065.2234983/150‑1 (MQ=60)
                                                           GAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGA                                                                                        <  SRR19973065.2234616/150‑1 (MQ=60)
                                                            AATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAA                                                                                       >  SRR19973065.133239/1‑150 (MQ=60)
                                                             ATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAAT                                                                                      <  SRR19973065.2288388/150‑1 (MQ=60)
                                                              TACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATT                                                                                     <  SRR19973065.1716887/150‑1 (MQ=60)
                                                              TACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATT                                                                                     <  SRR19973065.3511294/150‑1 (MQ=60)
                                                              TACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATT                                                                                     <  SRR19973065.1185430/150‑1 (MQ=60)
                                                              TACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATT                                                                                     <  SRR19973065.1186100/150‑1 (MQ=60)
                                                                CCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGA                                                                                   >  SRR19973065.1665439/1‑150 (MQ=60)
                                                                CCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGA                                                                                   <  SRR19973065.1479344/150‑1 (MQ=60)
                                                                 CCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGAT                                                                                  <  SRR19973065.2726990/150‑1 (MQ=60)
                                                                 CCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGAT                                                                                  >  SRR19973065.3465965/1‑150 (MQ=60)
                                                                 CCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGAT                                                                                  <  SRR19973065.958537/150‑1 (MQ=60)
                                                                  CCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATC                                                                                 <  SRR19973065.1552420/150‑1 (MQ=60)
                                                                    AGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTNCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCA                                                                               >  SRR19973065.1795053/1‑150 (MQ=60)
                                                                    AGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCA                                                                               >  SRR19973065.3044/1‑150 (MQ=60)
                                                                    AGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCA                                                                               <  SRR19973065.1755587/150‑1 (MQ=60)
                                                                    AGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCA                                                                               <  SRR19973065.3270677/150‑1 (MQ=60)
                                                                       ATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGT                                                                            <  SRR19973065.1964815/150‑1 (MQ=60)
                                                                       ATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGT                                                                            <  SRR19973065.1964458/150‑1 (MQ=60)
                                                                       ATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGT                                                                            <  SRR19973065.1964590/150‑1 (MQ=60)
                                                                       ATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGT                                                                            <  SRR19973065.1964704/150‑1 (MQ=60)
                                                                         TACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTG                                                                          >  SRR19973065.2282708/1‑150 (MQ=60)
                                                                         TACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTG                                                                          >  SRR19973065.2282916/1‑150 (MQ=60)
                                                                              ACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAT                                                                     <  SRR19973065.1778555/150‑1 (MQ=60)
                                                                                    CAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTA                                                               >  SRR19973065.1572636/1‑150 (MQ=60)
                                                                                     AATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAG                                                              >  SRR19973065.1509196/1‑150 (MQ=60)
                                                                                      ATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGT                                                             >  SRR19973065.1920150/1‑150 (MQ=60)
                                                                                       TATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTT                                                            <  SRR19973065.2451220/150‑1 (MQ=60)
                                                                                       TATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTT                                                            <  SRR19973065.100262/150‑1 (MQ=60)
                                                                                       TATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTT                                                            <  SRR19973065.2451205/150‑1 (MQ=60)
                                                                                       TATGGAGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTT                                                            <  SRR19973065.100260/150‑1 (MQ=60)
                                                                                         TGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCG                                                          <  SRR19973065.1518218/150‑1 (MQ=60)
                                                                                            TGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCA                                                       >  SRR19973065.1242148/1‑150 (MQ=60)
                                                                                             GAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCAT                                                      <  SRR19973065.1560993/150‑1 (MQ=60)
                                                                                             GAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCAT                                                      <  SRR19973065.1272826/150‑1 (MQ=60)
                                                                                             GAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCAT                                                      <  SRR19973065.1276235/150‑1 (MQ=60)
                                                                                              AAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATA                                                     >  SRR19973065.3381614/1‑150 (MQ=60)
                                                                                                GCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAAT                                                   <  SRR19973065.444546/150‑1 (MQ=60)
                                                                                                GCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAAT                                                   <  SRR19973065.2833855/150‑1 (MQ=60)
                                                                                                GCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAAT                                                   <  SRR19973065.445056/150‑1 (MQ=60)
                                                                                                    TGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTG                                               >  SRR19973065.205284/1‑150 (MQ=60)
                                                                                                    TGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTG                                               >  SRR19973065.3441217/1‑150 (MQ=60)
                                                                                                    TGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTG                                               >  SRR19973065.1066394/1‑150 (MQ=60)
                                                                                                    TGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTG                                               >  SRR19973065.204376/1‑150 (MQ=60)
                                                                                                    TGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTG                                               >  SRR19973065.205037/1‑150 (MQ=60)
                                                                                                     GGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGA                                              >  SRR19973065.1715422/1‑150 (MQ=60)
                                                                                                       ATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATG                                            >  SRR19973065.2565896/1‑150 (MQ=60)
                                                                                                        TGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGG                                           >  SRR19973065.10396/1‑150 (MQ=60)
                                                                                                         GATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGC                                          >  SRR19973065.1429032/1‑150 (MQ=60)
                                                                                                          ATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCG                                         <  SRR19973065.3171557/150‑1 (MQ=60)
                                                                                                          ATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCG                                         >  SRR19973065.1063678/1‑150 (MQ=60)
                                                                                                          ATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCG                                         >  SRR19973065.1063667/1‑150 (MQ=60)
                                                                                                          ATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCG                                         >  SRR19973065.947450/1‑150 (MQ=60)
                                                                                                            CTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTT                                       <  SRR19973065.1598719/150‑1 (MQ=60)
                                                                                                              CTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTAT                                     >  SRR19973065.8306/1‑150 (MQ=60)
                                                                                                               TTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATT                                    <  SRR19973065.2550734/150‑1 (MQ=60)
                                                                                                                 AACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGC                                  >  SRR19973065.1536131/1‑150 (MQ=60)
                                                                                                                    CGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAAC                               <  SRR19973065.2175842/150‑1 (MQ=60)
                                                                                                                        ATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAAT                           >  SRR19973065.974786/1‑150 (MQ=60)
                                                                                                                           AAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACC                        >  SRR19973065.679313/1‑150 (MQ=60)
                                                                                                                            AAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCA                       >  SRR19973065.3211805/1‑150 (MQ=60)
                                                                                                                             AAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCAC                      >  SRR19973065.3251184/1‑150 (MQ=60)
                                                                                                                              AATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACA                     <  SRR19973065.2253876/150‑1 (MQ=60)
                                                                                                                                TAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACT                   <  SRR19973065.2069188/150‑1 (MQ=60)
                                                                                                                                      AAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCG             <  SRR19973065.949096/150‑1 (MQ=60)
                                                                                                                                       AATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGT            >  SRR19973065.784733/1‑150 (MQ=60)
                                                                                                                                       AATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGT            >  SRR19973065.1139190/1‑150 (MQ=60)
                                                                                                                                          GATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTAGATCGTTCT         <  SRR19973065.1056438/150‑1 (MQ=60)
                                                                                                                                            TTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCT       >  SRR19973065.3186888/1‑150 (MQ=60)
                                                                                                                                             TTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTT      <  SRR19973065.729817/150‑1 (MQ=60)
                                                                                                                                             TTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTT      <  SRR19973065.2467228/150‑1 (MQ=60)
                                                                                                                                              TGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTG     >  SRR19973065.580633/1‑150 (MQ=60)
                                                                                                                                              TGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTG     >  SRR19973065.2355584/1‑150 (MQ=60)
                                                                                                                                                CAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTT   >  SRR19973065.3391623/1‑150 (MQ=60)
                                                                                                                                                 AAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTC  >  SRR19973065.1939564/1‑150 (MQ=60)
                                                                                                                                                 AAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTC  >  SRR19973065.3062295/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                 
ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTC  >  CP009273/1000692‑1000986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑