Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,000,841 G→T 100% A206A (GCG→GCT)  pyrD → dihydro‑orotate oxidase, FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,000,8410GT98.8% 804.9 / 0.5 248A206A (GCG→GCT) pyrDdihydro‑orotate oxidase, FMN‑linked
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (114/131);  total (115/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 248 total aligned reads displayed.

ATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTT  >  CP009273/1000698‑1000985
                                                                                                                                               |                                                                                                                                                
aTGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGc                                                                                                                                              <  1:2824365/148‑1 (MQ=255)
   gAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAcc                                                                                                                                           <  2:1374340/148‑1 (MQ=255)
   gAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAcc                                                                                                                                           >  2:697227/1‑148 (MQ=255)
   gAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCGGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAcc                                                                                                                                           >  1:2249683/1‑148 (MQ=255)
    aaaaaaTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCa                                                                                                                                          <  1:1284645/148‑1 (MQ=255)
       aaaTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATaa                                                                                                                                       <  1:3073279/148‑1 (MQ=255)
       aaaTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATaa                                                                                                                                       <  1:1420053/148‑1 (MQ=255)
         aTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAat                                                                                                                                     <  1:1611342/148‑1 (MQ=255)
         aTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAat                                                                                                                                     <  1:1611458/148‑1 (MQ=255)
         aTCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAat                                                                                                                                     <  1:3590395/148‑1 (MQ=255)
          tCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAata                                                                                                                                    >  1:3787996/1‑148 (MQ=255)
             atgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtgt                                                                                                                                 >  2:824085/1‑148 (MQ=255)
             atgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtgt                                                                                                                                 >  2:397714/1‑148 (MQ=255)
             atgcctatgcGGGATATAACGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACAATAAATAtgt                                                                                                                                 >  1:621815/1‑148 (MQ=255)
              tgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtgtg                                                                                                                                <  1:3488362/148‑1 (MQ=255)
               gcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGc                                                                                                                               <  2:460285/148‑1 (MQ=255)
                  tatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGa                                                                                                                            <  1:1029169/148‑1 (MQ=255)
                        ggATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAg                                                                                                                      <  2:2832664/148‑1 (MQ=255)
                        ggATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAg                                                                                                                      >  1:3055854/1‑148 (MQ=255)
                         gATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGt                                                                                                                     >  2:1686378/1‑148 (MQ=255)
                           tatatCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGa                                                                                                                   <  1:3725438/148‑1 (MQ=255)
                              atCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGa                                                                                                                >  2:3625711/1‑148 (MQ=255)
                              atCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGa                                                                                                                <  2:1039837/148‑1 (MQ=255)
                              atCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGa                                                                                                                >  2:1945799/1‑148 (MQ=255)
                               tCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGAt                                                                                                               >  2:3095137/1‑148 (MQ=255)
                                 gCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATcg                                                                                                             <  1:2075529/148‑1 (MQ=255)
                                  ccATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATcgc                                                                                                            >  2:3802134/1‑148 (MQ=255)
                                    aTCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCgcgc                                                                                                          >  1:3272500/1‑148 (MQ=255)
                                     tCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGcc                                                                                                         >  2:3036073/1‑148 (MQ=255)
                                      cAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCg                                                                                                        >  1:2325208/1‑148 (MQ=255)
                                         tatTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGAt                                                                                                     <  1:2978266/148‑1 (MQ=255)
                                          atTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATc                                                                                                    >  2:2126579/1‑148 (MQ=255)
                                          atTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATc                                                                                                    >  1:2455767/1‑148 (MQ=255)
                                          atTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATc                                                                                                    >  2:1970145/1‑148 (MQ=255)
                                           tttCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCt                                                                                                   >  1:849867/1‑148 (MQ=255)
                                            ttCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCACAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCtt                                                                                                  >  2:494208/1‑148 (MQ=255)
                                             tCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCttt                                                                                                 >  2:2236329/1‑148 (MQ=255)
                                             tCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCttt                                                                                                 <  1:3361410/148‑1 (MQ=255)
                                             tCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCttt                                                                                                 <  1:2103381/148‑1 (MQ=255)
                                             tCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCttt                                                                                                 <  2:3792583/148‑1 (MQ=255)
                                                      aaTACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaag                                                                                        <  2:743098/148‑1 (MQ=255)
                                                        tACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaagaa                                                                                      <  1:2883240/148‑1 (MQ=255)
                                                           cccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTg                                                                                   <  1:1845853/148‑1 (MQ=255)
                                                            ccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGa                                                                                  <  1:3391800/148‑1 (MQ=255)
                                                              aGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATc                                                                                <  1:495411/148‑1 (MQ=255)
                                                                gATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCa                                                                              >  1:1370024/1‑148 (MQ=255)
                                                                    acgcacgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGtt                                                                          >  2:188343/1‑148 (MQ=255)
                                                                       cacgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGcc                                                                       >  1:1209/1‑148 (MQ=255)
                                                                       cacgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGcc                                                                       >  2:1860959/1‑148 (MQ=255)
                                                                        acgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCg                                                                      <  2:3158527/148‑1 (MQ=255)
                                                                           cTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAta                                                                   <  2:2232353/148‑1 (MQ=255)
                                                                             gCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagt                                                                 >  2:3342905/1‑148 (MQ=255)
                                                                             gCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagt                                                                 >  2:20844/1‑148 (MQ=255)
                                                                              cAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtt                                                                <  2:3737222/148‑1 (MQ=255)
                                                                                 tatGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttag                                                             <  2:621815/148‑1 (MQ=255)
                                                                                   tGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttagtt                                                           <  2:2249643/148‑1 (MQ=255)
                                                                                   tGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttagtt                                                           >  1:252840/1‑148 (MQ=255)
                                                                                    ggTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTc                                                          >  1:823208/1‑148 (MQ=255)
                                                                                      tGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGTTCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGc                                                        <  2:871219/148‑1 (MQ=255)
                                                                                       gAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGcc                                                       <  2:151792/148‑1 (MQ=255)
                                                                                          gcgcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCata                                                    >  2:259457/1‑148 (MQ=255)
                                                                                          gcgcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCata                                                    >  1:191089/1‑148 (MQ=255)
                                                                                           cgcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataa                                                   <  2:143444/148‑1 (MQ=255)
                                                                                           cgcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataa                                                   <  1:999554/148‑1 (MQ=255)
                                                                                            gcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataat                                                  <  1:644406/148‑1 (MQ=255)
                                                                                             cTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataata                                                 <  1:526309/148‑1 (MQ=255)
                                                                                                gatgatCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTg                                              >  1:1706342/1‑148 (MQ=255)
                                                                                                 atgatCTCTTAACCGCGATTAAAAATAAGCAAACTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGa                                             <  2:1983070/148‑1 (MQ=255)
                                                                                                     tctcTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGc                                         >  2:3745172/1‑148 (MQ=255)
                                                                                                        ctTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGtt                                      >  1:244630/1‑148 (MQ=255)
                                                                                                        ctTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGtt                                      >  2:2941220/1‑148 (MQ=255)
                                                                                                        ctTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGtt                                      >  1:2172101/1‑148 (MQ=255)
                                                                                                        ctTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGtt                                      >  2:601556/1‑148 (MQ=255)
                                                                                                         ttAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTa                                     <  2:1146116/148‑1 (MQ=255)
                                                                                                         taaaCCGCTATTAAATATAAGCTAAATTATTTGCAAGCTATGCAACATAAATATGTGACTATCTAATTGAAGATCTCGCCGGATCTTTCTGAAGAAGTATTGATACAGGTTTCAGATAGTTTAGTTGGCCATAATATTGATGGCGTTa                                     <  1:1497114/146‑1 (MQ=255)
                                                                                                           aaCCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTAtt                                   >  1:267176/1‑148 (MQ=255)
                                                                                                            aCCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTg                                  >  2:2343962/1‑148 (MQ=255)
                                                                                                             ccGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGc                                 >  1:1072273/1‑148 (MQ=255)
                                                                                                                cgATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCacc                              >  2:1028659/1‑146 (MQ=255)
                                                                                                                  aTTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCa                            >  2:2311814/1‑148 (MQ=255)
                                                                                                                  aTTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCa                            <  1:3748373/148‑1 (MQ=255)
                                                                                                                     aaaaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATa                         <  2:3376683/148‑1 (MQ=255)
                                                                                                                      aaaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAc                        <  1:2293643/148‑1 (MQ=255)
                                                                                                                       aaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAcc                       >  2:1738232/1‑148 (MQ=255)
                                                                                                                       aaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAcc                       >  2:2774372/1‑148 (MQ=255)
                                                                                                                       aaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAcc                       >  2:1794453/1‑148 (MQ=255)
                                                                                                                          tAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACcaca                    <  2:3055854/148‑1 (MQ=255)
                                                                                                                           aaGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCacac                   >  2:1461472/1‑148 (MQ=255)
                                                                                                                                  aTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCg            >  1:2757055/1‑148 (MQ=255)
                                                                                                                                  aTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCg            >  2:399349/1‑148 (MQ=255)
                                                                                                                                  aTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCg            <  1:1101189/148‑1 (MQ=255)
                                                                                                                                   tGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGt           >  2:2557413/1‑148 (MQ=255)
                                                                                                                                   tGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGt           >  1:1513350/1‑148 (MQ=255)
                                                                                                                                    gATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGtt          >  2:2882082/1‑148 (MQ=255)
                                                                                                                                    gATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGtt          <  2:2487378/148‑1 (MQ=255)
                                                                                                                                     aTTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtc         <  1:1945799/148‑1 (MQ=255)
                                                                                                                                       ttGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtctc       <  1:2988203/148‑1 (MQ=255)
                                                                                                                                       ttGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtctc       <  2:2020663/148‑1 (MQ=255)
                                                                                                                                        tGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTctct      <  2:3177862/148‑1 (MQ=255)
                                                                                                                                        tGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTctct      <  2:2978757/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
ATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTT  >  CP009273/1000698‑1000985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGG  >  CP009273/1000695‑1000989
                                                                                                                                                  |                                                                                                                                                    
TGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATG                                                                                                                                                   <  SRR19973069.224851/150‑1 (MQ=60)
TGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATG                                                                                                                                                   <  SRR19973069.1974183/150‑1 (MQ=60)
   ATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAC                                                                                                                                                <  SRR19973069.2843042/150‑1 (MQ=60)
    TGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCGGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACC                                                                                                                                               >  SRR19973069.2264814/1‑150 (MQ=60)
    TGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCGGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACC                                                                                                                                               >  SRR19973069.2264854/1‑150 (MQ=60)
     GGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCA                                                                                                                                              <  SRR19973069.646926/150‑1 (MQ=60)
      GAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCAT                                                                                                                                             <  SRR19973069.2012245/150‑1 (MQ=60)
       AAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATA                                                                                                                                            <  SRR19973069.1293236/150‑1 (MQ=60)
       AAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATA                                                                                                                                            >  SRR19973069.2552742/1‑150 (MQ=60)
       AAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATA                                                                                                                                            <  SRR19973069.1292558/150‑1 (MQ=60)
          AAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAAT                                                                                                                                         <  SRR19973069.3093573/150‑1 (MQ=60)
          AAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAAT                                                                                                                                         <  SRR19973069.1429972/150‑1 (MQ=60)
          AAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAAT                                                                                                                                         <  SRR19973069.1430019/150‑1 (MQ=60)
           AATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATA                                                                                                                                        >  SRR19973069.3811391/1‑150 (MQ=60)
           AATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATA                                                                                                                                        >  SRR19973069.3812563/1‑150 (MQ=60)
           AATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATA                                                                                                                                        >  SRR19973069.3398756/1‑150 (MQ=60)
           AATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATA                                                                                                                                        <  SRR19973069.1615182/150‑1 (MQ=60)
            ATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAT                                                                                                                                       <  SRR19973069.1622170/150‑1 (MQ=60)
            ATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAT                                                                                                                                       >  SRR19973069.2695348/1‑150 (MQ=60)
            ATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAT                                                                                                                                       <  SRR19973069.1622289/150‑1 (MQ=60)
            ATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAT                                                                                                                                       <  SRR19973069.1622194/150‑1 (MQ=60)
            ATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAT                                                                                                                                       <  SRR19973069.3613818/150‑1 (MQ=60)
             TCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATG                                                                                                                                      <  SRR19973069.1773157/150‑1 (MQ=60)
              CTATGCCTATGCGGGATATAACGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACAATAAATATGT                                                                                                                                     >  SRR19973069.625949/1‑150 (MQ=60)
                 TGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCC                                                                                                                                  <  SRR19973069.3510866/150‑1 (MQ=60)
                 TGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCC                                                                                                                                  <  SRR19973069.3511429/150‑1 (MQ=60)
                  GCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCG                                                                                                                                 <  SRR19973069.1609586/150‑1 (MQ=60)
                     TATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATC                                                                                                                              <  SRR19973069.1036300/150‑1 (MQ=60)
                     TATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATC                                                                                                                              >  SRR19973069.2152097/1‑150 (MQ=60)
                     TATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATC                                                                                                                              <  SRR19973069.530995/150‑1 (MQ=60)
                      ATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCG                                                                                                                             <  SRR19973069.163760/150‑1 (MQ=60)
                         CGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAG                                                                                                                          >  SRR19973069.3076070/1‑150 (MQ=60)
                         CGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAG                                                                                                                          <  SRR19973069.2514377/150‑1 (MQ=60)
                             ATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAA                                                                                                                      >  SRR19973069.3198535/1‑150 (MQ=60)
                              TATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAG                                                                                                                     <  SRR19973069.3749746/150‑1 (MQ=60)
                                 ATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGATGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATC                                                                                                                  <  SRR19973069.1922269/150‑1 (MQ=60)
                                    GCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCG                                                                                                               <  SRR19973069.1860142/150‑1 (MQ=60)
                                    GCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCG                                                                                                               <  SRR19973069.2089900/150‑1 (MQ=60)
                                     CCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGC                                                                                                              >  SRR19973069.3293926/1‑150 (MQ=60)
                                      CATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCC                                                                                                             <  SRR19973069.3751572/150‑1 (MQ=60)
                                      CATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCC                                                                                                             <  SRR19973069.3751576/150‑1 (MQ=60)
                                       ATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCG                                                                                                            >  SRR19973069.2341241/1‑150 (MQ=60)
                                       ATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCG                                                                                                            >  SRR19973069.2341021/1‑150 (MQ=60)
                                       ATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCG                                                                                                            <  SRR19973069.3380320/150‑1 (MQ=60)
                                       ATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCG                                                                                                            >  SRR19973069.2942471/1‑150 (MQ=60)
                                           ATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATC                                                                                                        >  SRR19973069.2472273/1‑150 (MQ=60)
                                           ATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATC                                                                                                        >  SRR19973069.2470019/1‑150 (MQ=60)
                                            TATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCT                                                                                                       <  SRR19973069.2998040/150‑1 (MQ=60)
                                            TATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCT                                                                                                       >  SRR19973069.2539320/1‑150 (MQ=60)
                                            TATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCT                                                                                                       >  SRR19973069.855828/1‑150 (MQ=60)
                                            TATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCT                                                                                                       >  SRR19973069.855815/1‑150 (MQ=60)
                                            TATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCT                                                                                                       <  SRR19973069.2815864/150‑1 (MQ=60)
                                            TATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCT                                                                                                       >  SRR19973069.610625/1‑150 (MQ=60)
                                             ATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTT                                                                                                      >  SRR19973069.3839457/1‑150 (MQ=60)
                                                TCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCT                                                                                                   <  SRR19973069.3501068/150‑1 (MQ=60)
                                                TCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCT                                                                                                   <  SRR19973069.3383401/150‑1 (MQ=60)
                                                TCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCT                                                                                                   <  SRR19973069.2117900/150‑1 (MQ=60)
                                                 CATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTG                                                                                                  <  SRR19973069.1158237/150‑1 (MQ=60)
                                                 CATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTG                                                                                                  <  SRR19973069.3076365/150‑1 (MQ=60)
                                                 CATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTG                                                                                                  <  SRR19973069.680058/150‑1 (MQ=60)
                                                          ATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAAT                                                                                         <  SRR19973069.400621/150‑1 (MQ=60)
                                                           TACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATT                                                                                        <  SRR19973069.2902202/150‑1 (MQ=60)
                                                           TACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATT                                                                                        <  SRR19973069.2902266/150‑1 (MQ=60)
                                                           TACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATT                                                                                        >  SRR19973069.2998532/1‑150 (MQ=60)
                                                              CCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGAT                                                                                     >  SRR19973069.3545118/1‑150 (MQ=60)
                                                              CCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGAT                                                                                     <  SRR19973069.1858756/150‑1 (MQ=60)
                                                               CCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATC                                                                                    <  SRR19973069.3413950/150‑1 (MQ=60)
                                                               CCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATC                                                                                    <  SRR19973069.3413874/150‑1 (MQ=60)
                                                               CCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATC                                                                                    <  SRR19973069.983749/150‑1 (MQ=60)
                                                                 AGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCA                                                                                  >  SRR19973069.1379555/1‑150 (MQ=60)
                                                                 AGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCA                                                                                  <  SRR19973069.3184393/150‑1 (MQ=60)
                                                                 AGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCA                                                                                  <  SRR19973069.498966/150‑1 (MQ=60)
                                                                 AGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCA                                                                                  <  SRR19973069.2152451/150‑1 (MQ=60)
                                                                     TTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTT                                                                              <  SRR19973069.1698149/150‑1 (MQ=60)
                                                                        CGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCC                                                                           >  SRR19973069.1941/1‑150 (MQ=60)
                                                                        CGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCC                                                                           <  SRR19973069.1315019/150‑1 (MQ=60)
                                                                        CGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCC                                                                           >  SRR19973069.1231/1‑150 (MQ=60)
                                                                           ACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAT                                                                        <  SRR19973069.305126/150‑1 (MQ=60)
                                                                                 CAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTA                                                                  <  SRR19973069.997871/150‑1 (MQ=60)
                                                                                    TATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTT                                                               >  SRR19973069.254688/1‑150 (MQ=60)
                                                                                     ATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTC                                                              >  SRR19973069.829014/1‑150 (MQ=60)
                                                                                     ATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTC                                                              <  SRR19973069.1376471/150‑1 (MQ=60)
                                                                                           AAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGTCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATA                                                        >  SRR19973069.509004/1‑150 (MQ=60)
                                                                                           AAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATA                                                        >  SRR19973069.192648/1‑150 (MQ=60)
                                                                                              CGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATA                                                     <  SRR19973069.1006398/150‑1 (MQ=60)
                                                                                               GCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATAT                                                    <  SRR19973069.648638/150‑1 (MQ=60)
                                                                                                CTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATT                                                   <  SRR19973069.530031/150‑1 (MQ=60)
                                                                                                 TGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTG                                                  >  SRR19973069.1718286/1‑150 (MQ=60)
                                                                                                       ATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCG                                            >  SRR19973069.304586/1‑150 (MQ=60)
                                                                                                         CTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTT                                          >  SRR19973069.822333/1‑150 (MQ=60)
                                                                                                         CTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTT                                          >  SRR19973069.2186931/1‑150 (MQ=60)
                                                                                                         CTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTT                                          >  SRR19973069.246449/1‑150 (MQ=60)
                                                                                                            TTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATT                                       >  SRR19973069.269082/1‑150 (MQ=60)
                                                                                                            TAAACCGCTATTAAATATAAGCTAAATTATTTGCAAGCTATGCAACATAAATATGTGACTATCTAATTGAAGATCTCGCCGGATCTTTCTGAAGAAGTATTGATACAGGTTTCAGATAGTTTAGTTGGCCATAATATTGATGGCGTTATT                                       <  SRR19973069.1507492/150‑1 (MQ=58)
                                                                                                              AACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGC                                     >  SRR19973069.1381960/1‑150 (MQ=60)
                                                                                                              AACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGC                                     >  SRR19973069.1079752/1‑150 (MQ=60)
                                                                                                                     ATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAAT                              >  SRR19973069.3667268/1‑150 (MQ=60)
                                                                                                                     ATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAAT                              >  SRR19973069.2894805/1‑150 (MQ=60)
                                                                                                                     ATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAAT                              <  SRR19973069.3772784/150‑1 (MQ=60)
                                                                                                                        AAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACC                           <  SRR19973069.1110960/150‑1 (MQ=60)
                                                                                                                         AAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCA                          <  SRR19973069.2309231/150‑1 (MQ=60)
                                                                                                                            ATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACAC                       >  SRR19973069.2360934/1‑150 (MQ=60)
                                                                                                                             TAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACT                      >  SRR19973069.107200/1‑150 (MQ=60)
                                                                                                                              AAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTC                     >  SRR19973069.1962915/1‑150 (MQ=60)
                                                                                                                               AGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCG                    <  SRR19973069.2298847/150‑1 (MQ=60)
                                                                                                                               AGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCG                    <  SRR19973069.2313440/150‑1 (MQ=60)
                                                                                                                                  AAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATC                 <  SRR19973069.1470436/150‑1 (MQ=60)
                                                                                                                                  AAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATC                 >  SRR19973069.2702039/1‑150 (MQ=60)
                                                                                                                                   AAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCG                >  SRR19973069.2775461/1‑150 (MQ=60)
                                                                                                                                    AATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGT               >  SRR19973069.1523804/1‑150 (MQ=60)
                                                                                                                                    AATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGT               >  SRR19973069.1523792/1‑150 (MQ=60)
                                                                                                                                     ATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTT              <  SRR19973069.1108074/150‑1 (MQ=60)
                                                                                                                                     ATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTT              <  SRR19973069.1108885/150‑1 (MQ=60)
                                                                                                                                       GATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCT            <  SRR19973069.2294423/150‑1 (MQ=60)
                                                                                                                                        ATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTC           <  SRR19973069.1959227/150‑1 (MQ=60)
                                                                                                                                          TTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTT         <  SRR19973069.3008050/150‑1 (MQ=60)
                                                                                                                                            GCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGT       >  SRR19973069.2109106/1‑150 (MQ=60)
                                                                                                                                              AAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTC     >  SRR19973069.2169728/1‑150 (MQ=60)
                                                                                                                                                 CTATGCACCATAAATCTGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGG  <  SRR19973069.1757204/150‑1 (MQ=60)
                                                                                                                                                 CTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGG  >  SRR19973069.2047136/1‑150 (MQ=60)
                                                                                                                                                 CTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGG  >  SRR19973069.2044612/1‑150 (MQ=60)
                                                                                                                                                 CTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGG  >  SRR19973069.236443/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
TGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGG  >  CP009273/1000695‑1000989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑