Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F488 I0 R1	1275	300.1	10328444	91.0%	9398884	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,000,841	G→T	100%	A206A (GCG→GCT)	pyrD →	dihydro‑orotate oxidase, FMN‑linked

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,000,841	0	G	T	98.1%	822.2 / 4.8	256	A206A (GCG→GCT)	pyrD	dihydro‑orotate oxidase, FMN‑linked
Reads supporting (aligned to +/- strand): ref base G (2/3); new base T (128/123); total (130/126)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 256 total aligned reads displayed.

TTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCA  >  CP009273/1000694‑1000987

ttGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTAt                                                                                                                                                  <  1:4425497/150‑1 (MQ=255)

  gTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGc                                                                                                                                                >  2:1102820/1‑150 (MQ=255)

   tATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCa                                                                                                                                               <  1:343057/150‑1 (MQ=255)

   tATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCa                                                                                                                                               <  2:3032036/150‑1 (MQ=255)

    aTGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAc                                                                                                                                              >  1:2366815/1‑150 (MQ=255)

               ctatgcctatgcGGGATATATCGCCATCAATATTTCCTCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtgt                                                                                                                                   <  2:3075993/150‑1 (MQ=255)

                tatgcctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAtgtg                                                                                                                                  <  1:4222818/150‑1 (MQ=255)

                     ctatgcGGGATATATCGCCATCAATATTTCATCGCCGAATACACCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGAACCATAAATATGTGCCGAt                                                                                                                             >  2:2219946/1‑150 (MQ=255)

                       atgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCg                                                                                                                           <  2:4888421/150‑1 (MQ=255)

                       atgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCg                                                                                                                           >  2:562587/1‑150 (MQ=255)

                       atgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCg                                                                                                                           <  1:1072712/150‑1 (MQ=255)

                       atgcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCg                                                                                                                           <  1:1104061/150‑1 (MQ=255)

                         gcGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAAACGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCa                                                                                                                         >  1:1491188/1‑150 (MQ=255)

                          cGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAg                                                                                                                        <  2:3503944/150‑1 (MQ=255)

                            ggATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTg                                                                                                                      <  1:2727480/150‑1 (MQ=255)

                                 tatCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGAt                                                                                                                 <  1:2409850/150‑1 (MQ=255)

                                  atCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATc                                                                                                                <  1:4730707/150‑1 (MQ=255)

                                   tCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATcg                                                                                                               <  1:3671391/150‑1 (MQ=255)

                                    cGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATcgc                                                                                                              >  2:5151330/1‑150 (MQ=255)

                                      ccATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCgcgc                                                                                                            >  2:4300314/1‑150 (MQ=255)

                                       cATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGcc                                                                                                           >  1:3775861/1‑150 (MQ=255)

                                        aTCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCg                                                                                                          <  1:1168287/150‑1 (MQ=255)

                                          cAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCACCGGa                                                                                                        <  2:1180477/150‑1 (MQ=255)

                                           aaTATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGAt                                                                                                       <  1:2930281/150‑1 (MQ=255)

                                           aaTATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGAt                                                                                                       >  1:4033824/1‑150 (MQ=255)

                                            atatTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATc                                                                                                      <  1:1183969/150‑1 (MQ=255)

                                             tatTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCt                                                                                                     >  2:630138/1‑150 (MQ=255)

                                               tttCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCttt                                                                                                   <  1:4877937/150‑1 (MQ=255)

                                                ttCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTc                                                                                                  >  1:520371/1‑150 (MQ=255)

                                                 tCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCt                                                                                                 <  1:3331757/150‑1 (MQ=255)

                                                 tCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCt                                                                                                 <  2:1268830/150‑1 (MQ=255)

                                                 tCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCt                                                                                                 >  2:3692009/1‑150 (MQ=255)

                                                 tCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCt                                                                                                 >  2:684603/1‑150 (MQ=255)

                                                  cATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTg                                                                                                <  2:2099411/150‑1 (MQ=255)

                                                    tCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaa                                                                                              >  2:1734278/1‑150 (MQ=255)

                                                    tCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaa                                                                                              >  2:2688957/1‑150 (MQ=255)

                                                       ccGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTgaagaa                                                                                           <  2:339302/150‑1 (MQ=255)

                                                            tACCCCAGGATTACGCACTCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAAtt                                                                                      <  2:1498362/150‑1 (MQ=255)

                                                            tACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAAtt                                                                                      >  2:2818531/1‑150 (MQ=255)

                                                               cccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGAt                                                                                   >  2:2259913/1‑150 (MQ=255)

                                                               cccAGGATTACGCACGCTGCAATATGGTGAAGCGATGGATGATCTCAAAAACGCGATGAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGAGAAGATCGCGCCGGATCTATCTGAAGAAGAATAGAt                                                                                   >  1:4560308/1‑150 (MQ=255)

                                                                ccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATc                                                                                  >  1:4827990/1‑150 (MQ=255)

                                                                ccAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATc                                                                                  >  2:2919720/1‑150 (MQ=255)

                                                                  aGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCa                                                                                <  1:1278676/150‑1 (MQ=255)

                                                                    gATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAgg                                                                              <  2:444462/150‑1 (MQ=255)

                                                                    gATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAgg                                                                              >  1:1925973/1‑150 (MQ=255)

                                                                      ttACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGtt                                                                            >  2:2040612/1‑150 (MQ=255)

                                                                        acgcacgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGc                                                                          <  2:3128045/150‑1 (MQ=255)

                                                                         cgcacgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGcc                                                                         <  2:289101/150‑1 (MQ=255)

                                                                            acgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAt                                                                      >  2:442495/1‑150 (MQ=255)

                                                                            acgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAt                                                                      >  1:4641263/1‑150 (MQ=255)

                                                                             cgcTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAta                                                                     >  1:4145968/1‑150 (MQ=255)

                                                                               cTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagt                                                                   >  2:653566/1‑150 (MQ=255)

                                                                               cTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagt                                                                   >  2:1636449/1‑150 (MQ=255)

                                                                                tccgatttGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtt                                                                  >  2:1441048/8‑150 (MQ=255)

                                                                                tGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAGAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtt                                                                  >  1:3916253/1‑150 (MQ=255)

                                                                                   aaTATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttag                                                               >  2:2353668/1‑150 (MQ=255)

                                                                                   aaTATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAtagtttag                                                               >  2:157376/1‑150 (MQ=255)

                                                                                         gTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGcc                                                         <  1:976018/150‑1 (MQ=255)

                                                                                          tGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCa                                                        <  1:637079/150‑1 (MQ=255)

                                                                                           gAATCGCTGGATGATCTCTTAACCGAGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAAGTGATCCAGGTTGCCGATAGTTTAGTTCGCCat                                                       >  1:1986081/1‑150 (MQ=255)

                                                                                           gAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCat                                                       <  2:88768/150‑1 (MQ=255)

                                                                                             aGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataa                                                     >  1:4296991/1‑150 (MQ=255)

                                                                                               cgcTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCataata                                                   >  2:4642204/1‑150 (MQ=255)

                                                                                                   ggATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGa                                               >  2:4526726/1‑150 (MQ=255)

                                                                                                   ggATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGa                                               >  2:2199689/1‑150 (MQ=255)

                                                                                                         tctcTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGt                                         <  1:2631923/150‑1 (MQ=255)

                                                                                                             ttAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTAtt                                     >  1:489277/1‑150 (MQ=255)

                                                                                                                aCCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCa                                  <  1:2484440/150‑1 (MQ=255)

                                                                                                                 ccGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCaa                                 <  2:1800403/150‑1 (MQ=255)

                                                                                                                   gcgATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAAcc                               >  2:3733890/1‑150 (MQ=255)

                                                                                                                     gATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCaa                             <  1:1586689/150‑1 (MQ=255)

                                                                                                                        tAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAc                          <  1:2143175/150‑1 (MQ=255)

                                                                                                                        tAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAc                          <  2:2508690/150‑1 (MQ=255)

                                                                                                                          aaaaaaAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACca                        >  2:167310/1‑150 (MQ=255)

                                                                                                                          aaaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACca                        <  2:153117/150‑1 (MQ=255)

                                                                                                                          aaaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACca                        >  1:5037624/1‑150 (MQ=255)

                                                                                                                          aaaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGGTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCACTACca                        >  2:1938329/1‑150 (MQ=255)

                                                                                                                           aaaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACcac                       >  1:2447338/1‑150 (MQ=255)

                                                                                                                            aaTAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACcaca                      >  1:4509308/1‑150 (MQ=255)

                                                                                                                              tAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACt                    >  2:4147154/1‑150 (MQ=255)

                                                                                                                              tAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACt                    <  1:54298/150‑1 (MQ=255)

                                                                                                                               aaGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTc                   <  1:1734017/150‑1 (MQ=255)

                                                                                                                               aaGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCAGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTc                   <  1:3405076/150‑1 (MQ=255)

                                                                                                                                aGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCg                  <  1:437760/150‑1 (MQ=255)

                                                                                                                                aGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCg                  <  1:862787/150‑1 (MQ=255)

                                                                                                                                aGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCg                  >  1:818086/1‑150 (MQ=255)

                                                                                                                                   taaaTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATc               >  1:994705/2‑150 (MQ=255)

                                                                                                                                   aaaaTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATc               <  1:3305531/150‑1 (MQ=255)

                                                                                                                                   aaaaTGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATc               <  2:4634766/150‑1 (MQ=255)

                                                                                                                                     aaTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGt             <  2:670839/150‑1 (MQ=255)

                                                                                                                                      aTGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGtt            <  2:45717/150‑1 (MQ=255)

                                                                                                                                         aTTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTtctc         <  1:2848095/150‑1 (MQ=255)

                                                                                                                                           ttGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCtt       <  2:3945549/150‑1 (MQ=255)

                                                                                                                                            tGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTg      <  1:680848/150‑1 (MQ=255)

                                                                                                                                              cAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAATAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGtt    <  1:2219946/150‑1 (MQ=255)

                                                                                                                                              cAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGtt    >  2:1979330/1‑150 (MQ=255)

                                                                                                                                              cAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGtt    >  1:3633551/1‑150 (MQ=255)

                                                                                                                                               aaGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTc   <  1:4696822/150‑1 (MQ=255)

                                                                                                                                               aaGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTc   >  1:1586545/1‑150 (MQ=255)

TTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCA  >  CP009273/1000694‑1000987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGG  >  CP009273/1000692‑1000990

ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCT                                                                                                                                                       >  SRR19973063.2073677/1‑150 (MQ=60)

  TTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTAT                                                                                                                                                     <  SRR19973063.4439701/150‑1 (MQ=60)

     TATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCA                                                                                                                                                  <  SRR19973063.344044/150‑1 (MQ=60)

      ATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCAC                                                                                                                                                 >  SRR19973063.2374397/1‑150 (MQ=60)

          AAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATA                                                                                                                                             >  SRR19973063.3648612/1‑150 (MQ=60)

          AAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATA                                                                                                                                             <  SRR19973063.1506270/150‑1 (MQ=60)

               ATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAT                                                                                                                                        >  SRR19973063.2862217/1‑150 (MQ=60)

               ATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATAT                                                                                                                                        >  SRR19973063.2862526/1‑150 (MQ=60)

                 CTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGT                                                                                                                                      >  SRR19973063.823181/1‑150 (MQ=60)

                 CTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGT                                                                                                                                      >  SRR19973063.823195/1‑150 (MQ=60)

                  TATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTG                                                                                                                                     <  SRR19973063.4236279/150‑1 (MQ=60)

                    TGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCC                                                                                                                                   >  SRR19973063.4008371/1‑150 (MQ=60)

                    TGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCC                                                                                                                                   >  SRR19973063.2279731/1‑150 (MQ=60)

                    TGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCC                                                                                                                                   >  SRR19973063.4008975/1‑150 (MQ=60)

                    TGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCC                                                                                                                                   <  SRR19973063.4211714/150‑1 (MQ=60)

                     GCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCG                                                                                                                                  <  SRR19973063.3642436/150‑1 (MQ=60)

                       CTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGAT                                                                                                                                <  SRR19973063.4899071/150‑1 (MQ=60)

                         ATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCG                                                                                                                              <  SRR19973063.1075969/150‑1 (MQ=60)

                         ATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCG                                                                                                                              <  SRR19973063.1107423/150‑1 (MQ=60)

                           GCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAAACGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCA                                                                                                                            >  SRR19973063.1495756/1‑150 (MQ=60)

                             GGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATTTGCCGATCGCAGT                                                                                                                          >  SRR19973063.4775229/1‑150 (MQ=60)

                              GGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTG                                                                                                                         <  SRR19973063.2736283/150‑1 (MQ=60)

                               GATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGA                                                                                                                        <  SRR19973063.2103738/150‑1 (MQ=60)

                                   TATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGAT                                                                                                                    <  SRR19973063.2417558/150‑1 (MQ=60)

                                   TATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGAT                                                                                                                    <  SRR19973063.2392454/150‑1 (MQ=60)

                                    ATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATC                                                                                                                   <  SRR19973063.4745783/150‑1 (MQ=60)

                                     TCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCG                                                                                                                  <  SRR19973063.3683149/150‑1 (MQ=60)

                                     TCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCG                                                                                                                  <  SRR19973063.3683204/150‑1 (MQ=60)

                                         CATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCC                                                                                                              >  SRR19973063.3787919/1‑150 (MQ=60)

                                          ATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCG                                                                                                             <  SRR19973063.1171807/150‑1 (MQ=60)

                                            CAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGA                                                                                                           >  SRR19973063.2676937/1‑150 (MQ=60)

                                             AATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGAT                                                                                                          <  SRR19973063.4714094/150‑1 (MQ=60)

                                             AATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGAT                                                                                                          <  SRR19973063.2480105/150‑1 (MQ=60)

                                             AATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGAT                                                                                                          <  SRR19973063.2939625/150‑1 (MQ=60)

                                             AATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGAT                                                                                                          >  SRR19973063.1455213/1‑150 (MQ=60)

                                             AATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGAT                                                                                                          >  SRR19973063.4046753/1‑150 (MQ=60)

                                              ATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATC                                                                                                         <  SRR19973063.1187536/150‑1 (MQ=60)

                                              ATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATC                                                                                                         <  SRR19973063.124492/150‑1 (MQ=60)

                                               TATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCT                                                                                                        <  SRR19973063.3439847/150‑1 (MQ=60)

                                                 TTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTT                                                                                                      <  SRR19973063.4893710/150‑1 (MQ=60)

                                                 TTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTT                                                                                                      <  SRR19973063.4893663/150‑1 (MQ=60)

                                                  TTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTC                                                                                                     >  SRR19973063.521894/1‑150 (MQ=60)

                                                  TTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTC                                                                                                     >  SRR19973063.521891/1‑150 (MQ=60)

                                                   TCATCGCCGAATACCCCAGGATTACTCACGCTGCAATATGGTGAAGCTCTGTATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACAATAAATATGTGCAGATCGCAGTGAATATCGCGCCGGATCTTTCT                                                                                                    >  SRR19973063.4121959/1‑150 (MQ=60)

                                                   TCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCT                                                                                                    <  SRR19973063.3342387/150‑1 (MQ=60)

                                                   TCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCT                                                                                                    <  SRR19973063.1516312/150‑1 (MQ=60)

                                                    CATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTG                                                                                                   <  SRR19973063.1227092/150‑1 (MQ=60)

                                                      TCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAA                                                                                                 >  SRR19973063.3430699/1‑150 (MQ=60)

                                                              TACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATT                                                                                         >  SRR19973063.4227960/1‑150 (MQ=60)

                                                               ACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGTATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTG                                                                                        <  SRR19973063.2009663/150‑1 (MQ=60)

                                                               ACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTG                                                                                        <  SRR19973063.4687346/150‑1 (MQ=60)

                                                                 CCCAGGATTACGCACGCTGCAATATGGTGAAGCGATGGATGATCTCAAAAACGCGATGAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGAGAAGATCGCGCCGGATCTATCTGAAGAAGAATAGAT                                                                                      >  SRR19973063.4574912/1‑150 (MQ=60)

                                                                  CCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATC                                                                                     >  SRR19973063.4843459/1‑150 (MQ=60)

                                                                    AGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCA                                                                                   <  SRR19973063.1282490/150‑1 (MQ=60)

                                                                    AGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCA                                                                                   <  SRR19973063.1781257/150‑1 (MQ=60)

                                                                    AGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCA                                                                                   <  SRR19973063.1781336/150‑1 (MQ=60)

                                                                      GATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGG                                                                                 >  SRR19973063.1931995/1‑150 (MQ=60)

                                                                      GATTACGCACGCTGCAATATGGAGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGG                                                                                 <  SRR19973063.3502679/150‑1 (MQ=60)

                                                                       ATTACGCACGCTGCAATATGGTGAAGCGCTGGATGANCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGT                                                                                >  SRR19973063.2685593/1‑150 (MQ=60)

                                                                        TTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTT                                                                               <  SRR19973063.3350939/150‑1 (MQ=60)

                                                                         TACGCACGCTGCAATTTGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTG                                                                              <  SRR19973063.5102752/150‑1 (MQ=60)

                                                                              ACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAT                                                                         >  SRR19973063.4656087/1‑150 (MQ=60)

                                                                              ACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAT                                                                         <  SRR19973063.2248161/150‑1 (MQ=60)

                                                                              ACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGAT                                                                         >  SRR19973063.4656090/1‑150 (MQ=60)

                                                                               CGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATA                                                                        >  SRR19973063.4159228/1‑150 (MQ=60)

                                                                                  TGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAGAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTT                                                                     >  SRR19973063.3928747/1‑150 (MQ=60)

                                                                                  TGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTT                                                                     <  SRR19973063.378106/150‑1 (MQ=60)

                                                                                        ATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTC                                                               <  SRR19973063.1958900/150‑1 (MQ=60)

                                                                                        ATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTC                                                               <  SRR19973063.4661847/150‑1 (MQ=60)

                                                                                           GTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCC                                                            <  SRR19973063.978961/150‑1 (MQ=60)

                                                                                            TGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCA                                                           <  SRR19973063.638956/150‑1 (MQ=60)

                                                                                             GAATCGCTGGATGATCTCTTAACCGAGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAAGTGATCCAGGTTGCCGATAGTTTAGTTCGCCAT                                                          >  SRR19973063.1992288/1‑150 (MQ=60)

                                                                                               AGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAA                                                        <  SRR19973063.4415460/150‑1 (MQ=60)

                                                                                               AGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAA                                                        >  SRR19973063.4310647/1‑150 (MQ=60)

                                                                                                GCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAAT                                                       <  SRR19973063.64364/150‑1 (MQ=60)

                                                                                                GCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAAT                                                       <  SRR19973063.64344/150‑1 (MQ=60)

                                                                                                 CGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATA                                                      >  SRR19973063.4334837/1‑150 (MQ=60)

                                                                                                  GCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATAT                                                     <  SRR19973063.4289035/150‑1 (MQ=60)

                                                                                                          ATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCG                                             >  SRR19973063.2442754/1‑150 (MQ=60)

                                                                                                          ATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCG                                             >  SRR19973063.2443722/1‑150 (MQ=60)

                                                                                                           TCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGT                                            <  SRR19973063.2640319/150‑1 (MQ=60)

                                                                                                               TTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATT                                        >  SRR19973063.490677/1‑150 (MQ=60)

                                                                                                                TAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTG                                       <  SRR19973063.1081657/150‑1 (MQ=60)

                                                                                                                TAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTG                                       <  SRR19973063.4370056/150‑1 (MQ=60)

                                                                                                                TAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTG                                       >  SRR19973063.1709063/1‑150 (MQ=60)

                                                                                                                TAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTG                                       >  SRR19973063.3064838/1‑150 (MQ=60)

                                                                                                                  ACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCA                                     >  SRR19973063.962224/1‑150 (MQ=60)

                                                                                                                  ACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCA                                     >  SRR19973063.962179/1‑150 (MQ=60)

                                                                                                                  ACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCA                                     <  SRR19973063.2492377/150‑1 (MQ=60)

                                                                                                                   CCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAA                                    >  SRR19973063.1133822/1‑150 (MQ=60)

                                                                                                                   CCGCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAA                                    <  SRR19973063.2609922/150‑1 (MQ=60)

                                                                                                                     GCGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGATGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACC                                  <  SRR19973063.3815782/150‑1 (MQ=60)

                                                                                                                      CGATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCA                                 >  SRR19973063.4561109/1‑150 (MQ=60)

                                                                                                                       GATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAA                                >  SRR19973063.541830/1‑150 (MQ=60)

                                                                                                                       GATTAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAA                                <  SRR19973063.1591547/150‑1 (MQ=60)

                                                                                                                          TAAAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATAC                             <  SRR19973063.2149860/150‑1 (MQ=60)

                                                                                                                            AAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCA                           >  SRR19973063.4532296/1‑150 (MQ=60)

                                                                                                                            AAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCA                           >  SRR19973063.560994/1‑150 (MQ=60)

                                                                                                                            AAAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCA                           >  SRR19973063.5053861/1‑150 (MQ=60)

                                                                                                                             AAATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCAC                          >  SRR19973063.2455152/1‑150 (MQ=60)

                                                                                                                              AATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACA                         >  SRR19973063.4523779/1‑150 (MQ=60)

                                                                                                                              AATAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACA                         <  SRR19973063.4862937/150‑1 (MQ=60)

                                                                                                                                TAAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACT                       <  SRR19973063.54489/150‑1 (MQ=60)

                                                                                                                                 AAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTC                      <  SRR19973063.1739298/150‑1 (MQ=60)

                                                                                                                                 AAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTC                      <  SRR19973063.4919390/150‑1 (MQ=60)

                                                                                                                                 AAGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCAGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTC                      <  SRR19973063.3415908/150‑1 (MQ=60)

                                                                                                                                  AGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCG                     <  SRR19973063.439135/150‑1 (MQ=60)

                                                                                                                                  AGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCG                     <  SRR19973063.438976/150‑1 (MQ=60)

                                                                                                                                  AGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCG                     <  SRR19973063.1186322/150‑1 (MQ=60)

                                                                                                                                  AGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCG                     >  SRR19973063.820450/1‑150 (MQ=60)

                                                                                                                                  AGCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCG                     <  SRR19973063.865272/150‑1 (MQ=60)

                                                                                                                                   GCAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAATTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGA                    >  SRR19973063.1716201/1‑150 (MQ=60)

                                                                                                                                    CAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGTTCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGAT                   <  SRR19973063.3336647/150‑1 (MQ=60)

                                                                                                                                    CAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGAT                   <  SRR19973063.1147497/150‑1 (MQ=60)

                                                                                                                                    CAAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGAT                   <  SRR19973063.3336660/150‑1 (MQ=60)

                                                                                                                                    CAAAATGATGTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGAT                   <  SRR19973063.4796087/150‑1 (MQ=60)

                                                                                                                                     TAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATC                  >  SRR19973063.997606/1‑150 (MQ=60)

                                                                                                                                     TAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATC                  >  SRR19973063.997706/1‑150 (MQ=60)

                                                                                                                                     AAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATC                  <  SRR19973063.4454224/150‑1 (MQ=60)

                                                                                                                                     AAAATGATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATC                  <  SRR19973063.3316105/150‑1 (MQ=60)

                                                                                                                                           ATTTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTC            <  SRR19973063.2857231/150‑1 (MQ=60)

                                                                                                                                             TTGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTT          <  SRR19973063.2206610/150‑1 (MQ=60)

                                                                                                                                              TGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTG         <  SRR19973063.682843/150‑1 (MQ=60)

                                                                                                                                              TGCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTG         <  SRR19973063.683041/150‑1 (MQ=60)

                                                                                                                                               GCAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGT        <  SRR19973063.2498404/150‑1 (MQ=60)

                                                                                                                                                CAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAATAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTT       <  SRR19973063.2226956/150‑1 (MQ=60)

                                                                                                                                                CAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTT       <  SRR19973063.5022384/150‑1 (MQ=60)

                                                                                                                                                CAAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTT       >  SRR19973063.3645209/1‑150 (MQ=60)

                                                                                                                                                 AAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTC      <  SRR19973063.4711796/150‑1 (MQ=60)

                                                                                                                                                 AAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTC      >  SRR19973063.1591403/1‑150 (MQ=60)

                                                                                                                                                 AAGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTC      >  SRR19973063.1591473/1‑150 (MQ=60)

                                                                                                                                                  AGCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCA     >  SRR19973063.2831840/1‑150 (MQ=60)

                                                                                                                                                   GCTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAG    >  SRR19973063.647916/1‑150 (MQ=60)

                                                                                                                                                    CTATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGG   <  SRR19973063.2124794/150‑1 (MQ=60)

                                                                                                                                                     TATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGG  >  SRR19973063.1454446/1‑150 (MQ=60)

ATTTGTATGGAAAAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGATTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAGCAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCGGATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGATGGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGG  >  CP009273/1000692‑1000990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`