Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,219,533 A→G 100% intergenic (+170/+202) ycgI → / ← minE pseudogene/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,219,5330AG99.5% 763.7 / ‑4.7 190intergenic (+170/+202)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (92/97);  total (92/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

GGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGAT  >  CP009273/1219388‑1219676
                                                                                                                                                 |                                                                                                                                               
ggCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGAt                                                                                                                                               <  1:3032707/148‑1 (MQ=255)
 gcgcAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATa                                                                                                                                              <  2:2527029/148‑1 (MQ=255)
  cgcAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATaa                                                                                                                                             <  2:2971814/148‑1 (MQ=255)
    cAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAgg                                                                                                                                           <  2:2669235/148‑1 (MQ=255)
      gTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGtt                                                                                                                                         <  2:3092998/148‑1 (MQ=255)
       ttttCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTa                                                                                                                                        >  2:151147/1‑148 (MQ=255)
         ttCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTAtt                                                                                                                                      >  2:2877566/1‑148 (MQ=255)
         ttCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCACAGTATATCCATACTAACGATAAGGTTAtt                                                                                                                                      >  1:3183956/1‑148 (MQ=255)
          tCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTAttt                                                                                                                                     <  1:2138554/148‑1 (MQ=255)
          tCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTAttt                                                                                                                                     <  1:2137969/148‑1 (MQ=255)
           cGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTa                                                                                                                                    <  2:336157/148‑1 (MQ=255)
           cGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTa                                                                                                                                    <  2:2813594/148‑1 (MQ=255)
           cGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTa                                                                                                                                    <  2:336699/148‑1 (MQ=255)
              ttACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTc                                                                                                                                 >  2:1345416/1‑148 (MQ=255)
                 cAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAAc                                                                                                                              >  1:2853613/1‑148 (MQ=255)
                 cAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAAc                                                                                                                              <  1:381057/148‑1 (MQ=255)
                   ggATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCa                                                                                                                            <  2:393760/148‑1 (MQ=255)
                        ttGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCAt                                                                                                                       >  2:246355/1‑148 (MQ=255)
                        ttGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCAt                                                                                                                       >  2:1915199/1‑148 (MQ=255)
                        ttGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCAt                                                                                                                       >  1:3244186/1‑148 (MQ=255)
                         tGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATa                                                                                                                      >  2:1578888/1‑148 (MQ=255)
                         tGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATa                                                                                                                      >  1:690192/1‑148 (MQ=255)
                         tGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATa                                                                                                                      >  1:2226721/1‑148 (MQ=255)
                          gAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATaa                                                                                                                     <  2:1515769/148‑1 (MQ=255)
                           aaaGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATaaa                                                                                                                    <  1:54926/148‑1 (MQ=255)
                              ggtaaaaTTCTGTGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGTGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAt                                                                                                                 <  1:1061956/145‑1 (MQ=255)
                              gggAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAt                                                                                                                 >  2:3118315/1‑148 (MQ=255)
                              gggAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAt                                                                                                                 >  2:1078047/1‑148 (MQ=255)
                                gAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttt                                                                                                               <  1:1402367/148‑1 (MQ=255)
                                  aaaTTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttttg                                                                                                             <  2:1268965/148‑1 (MQ=255)
                                   aaTTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttttgt                                                                                                            <  2:1992254/148‑1 (MQ=255)
                                   aaTTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttttgt                                                                                                            <  2:1181947/148‑1 (MQ=255)
                                       cTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttttgttttg                                                                                                        <  2:479646/148‑1 (MQ=255)
                                           ggCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCg                                                                                                    <  1:2993689/148‑1 (MQ=255)
                                                aaaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGa                                                                                               <  2:968177/148‑1 (MQ=255)
                                                    aGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAg                                                                                           >  1:435092/1‑148 (MQ=255)
                                                    aGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAg                                                                                           >  2:2892976/1‑148 (MQ=255)
                                                      cccGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGcc                                                                                         <  1:1193178/148‑1 (MQ=255)
                                                          ccAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTaa                                                                                     <  2:1899091/148‑1 (MQ=255)
                                                            aGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAgg                                                                                   <  2:2377369/148‑1 (MQ=255)
                                                            aGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAgg                                                                                   <  2:2377560/148‑1 (MQ=255)
                                                             gTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATGAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGgt                                                                                  <  1:607357/148‑1 (MQ=255)
                                                              ttACGGCGGGAAACCTCATCCTATGGCAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAATGTTATTTACTCAACCAGGCATAAACCTTTTGTTTTGTGCGTGGGAACAGCCTTAAGgtg                                                                                 >  2:2782350/1‑148 (MQ=255)
                                                               tACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGgtgt                                                                                >  1:537896/1‑148 (MQ=255)
                                                                aCGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTa                                                                               >  1:975712/1‑148 (MQ=255)
                                                                   gcggGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATCTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAg                                                                            <  2:1190877/148‑1 (MQ=255)
                                                                      ggAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAgggg                                                                         >  2:1019587/1‑148 (MQ=255)
                                                                      ggAAACCACATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAgggg                                                                         <  2:2477832/148‑1 (MQ=255)
                                                                       gAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggggg                                                                        <  2:1713470/148‑1 (MQ=255)
                                                                       gAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggggg                                                                        <  2:1713138/148‑1 (MQ=255)
                                                                        aaaCCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGa                                                                       <  1:1579257/148‑1 (MQ=255)
                                                                        aaaCCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGa                                                                       <  1:1578888/148‑1 (MQ=255)
                                                                          aCCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAgg                                                                     <  1:3102849/148‑1 (MQ=255)
                                                                             tCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTgg                                                                  <  2:2060327/148‑1 (MQ=255)
                                                                              cATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGa                                                                 >  2:2731900/1‑148 (MQ=255)
                                                                              cATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGa                                                                 >  1:1304403/1‑148 (MQ=255)
                                                                               aTCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGaa                                                                <  1:1345416/148‑1 (MQ=255)
                                                                                 ccTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCACCCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAAt                                                              <  1:1321943/148‑1 (MQ=255)
                                                                                 ccTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAAt                                                              <  1:2126764/148‑1 (MQ=255)
                                                                                    aTGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGc                                                           >  1:3173457/1‑148 (MQ=255)
                                                                                      gggAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCaa                                                         <  2:3114331/148‑1 (MQ=255)
                                                                                      gggAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCaa                                                         <  2:2172366/148‑1 (MQ=255)
                                                                                      gggAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCaa                                                         <  2:3183956/148‑1 (MQ=255)
                                                                                       ggAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAAAGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAAt                                                        >  2:2113302/1‑148 (MQ=255)
                                                                                        gagaACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATg                                                       >  2:2842187/1‑148 (MQ=255)
                                                                                        gagaACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATg                                                       <  2:1198281/148‑1 (MQ=255)
                                                                                          gaACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgag                                                     >  1:1614343/1‑148 (MQ=255)
                                                                                           aaCAATGAATAATGAAAGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgagg                                                    <  2:2774924/148‑1 (MQ=255)
                                                                                               aTGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTa                                                >  2:745281/1‑148 (MQ=255)
                                                                                                tGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAt                                               >  2:1481505/1‑148 (MQ=255)
                                                                                                tGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAt                                               >  1:327969/1‑148 (MQ=255)
                                                                                                tGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAt                                               >  1:1924771/1‑148 (MQ=255)
                                                                                                tGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAt                                               >  1:1975261/1‑148 (MQ=255)
                                                                                                tGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAt                                               >  2:2796076/1‑148 (MQ=255)
                                                                                                tGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAt                                               >  1:1698749/1‑148 (MQ=255)
                                                                                                 gAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATc                                              <  2:429259/148‑1 (MQ=255)
                                                                                                 gAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATc                                              <  1:612674/148‑1 (MQ=255)
                                                                                                 gAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATc                                              <  1:2274286/148‑1 (MQ=255)
                                                                                                    taatGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAATGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGc                                           >  2:670343/1‑148 (MQ=255)
                                                                                                       tGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAg                                        >  1:1844072/1‑148 (MQ=255)
                                                                                                        gAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGa                                       <  1:1097079/148‑1 (MQ=255)
                                                                                                           aTTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATa                                    <  1:2483345/148‑1 (MQ=255)
                                                                                                                 gggTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTcgcc                              >  2:1280468/1‑148 (MQ=255)
                                                                                                                   gTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTcgccgc                            <  2:791750/148‑1 (MQ=255)
                                                                                                                   gTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTcgccgc                            >  1:258108/1‑148 (MQ=255)
                                                                                                                     tATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAACTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCtt                          >  2:2453195/1‑148 (MQ=255)
                                                                                                                        catcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTCAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAc                       >  2:1925603/1‑148 (MQ=255)
                                                                                                                        catcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAc                       <  1:2359293/148‑1 (MQ=255)
                                                                                                                         atcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAcc                      >  1:120269/1‑148 (MQ=255)
                                                                                                                          tctcCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACca                     >  1:2858327/1‑148 (MQ=255)
                                                                                                                            tcCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACcaca                   <  2:2226721/148‑1 (MQ=255)
                                                                                                                                 gTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTg              >  1:209465/1‑148 (MQ=255)
                                                                                                                                  tataTCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTgg             <  1:1168224/148‑1 (MQ=255)
                                                                                                                                   atatCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGa            >  2:1133550/1‑148 (MQ=255)
                                                                                                                                      tCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATga         <  1:1484712/148‑1 (MQ=255)
                                                                                                                                       ccATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATgag        <  1:247129/148‑1 (MQ=255)
                                                                                                                                       ccATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATgag        <  2:1576494/148‑1 (MQ=255)
                                                                                                                                           aCTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAggg    >  1:3103069/1‑148 (MQ=255)
                                                                                                                                           aCTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAggg    >  1:176526/1‑148 (MQ=255)
                                                                                                                                            cTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGa   >  2:426965/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGAT  >  CP009273/1219388‑1219676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  CP009273/1219387‑1219682
                                                                                                                                                  |                                                                                                                                                     
CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATA                                                                                                                                                    <  SRR19973070.2902093/150‑1 (MQ=60)
 GGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAA                                                                                                                                                   <  SRR19973070.3052003/150‑1 (MQ=60)
    GCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGT                                                                                                                                                <  SRR19973070.649206/150‑1 (MQ=60)
    ACAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGT                                                                                                                                                <  SRR19973070.1999527/150‑1 (MQ=60)
        TTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCACAGTATATCCATACTAACGATAAGGTTATT                                                                                                                                            >  SRR19973070.3204556/1‑150 (MQ=60)
           TCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTAC                                                                                                                                         <  SRR19973070.2152820/150‑1 (MQ=60)
           TCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTAC                                                                                                                                         <  SRR19973070.2152235/150‑1 (MQ=60)
           TCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTAC                                                                                                                                         <  SRR19973070.2152202/150‑1 (MQ=60)
              TTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCA                                                                                                                                      >  SRR19973070.1315854/1‑150 (MQ=60)
                TACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAAC                                                                                                                                    >  SRR19973070.2872099/1‑150 (MQ=60)
                  CAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCA                                                                                                                                  <  SRR19973070.383852/150‑1 (MQ=60)
                   AGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAG                                                                                                                                 <  SRR19973070.2874477/150‑1 (MQ=60)
                       TGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCAT                                                                                                                             >  SRR19973070.3265099/1‑150 (MQ=60)
                        GTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATA                                                                                                                            >  SRR19973070.2241361/1‑150 (MQ=60)
                        GTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATA                                                                                                                            >  SRR19973070.2969041/1‑150 (MQ=60)
                        GTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATA                                                                                                                            >  SRR19973070.695121/1‑150 (MQ=60)
                            AAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACA                                                                                                                        >  SRR19973070.2278813/1‑150 (MQ=60)
                            AAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACA                                                                                                                        <  SRR19973070.55571/150‑1 (MQ=60)
                               GGTAAAATTCTGTGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGTGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTT                                                                                                                     <  SRR19973070.1069402/150‑1 (MQ=60)
                                 GAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTG                                                                                                                   <  SRR19973070.1411851/150‑1 (MQ=60)
                                 GAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTG                                                                                                                   <  SRR19973070.1411836/150‑1 (MQ=60)
                                            GGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTG                                                                                                        <  SRR19973070.3012861/150‑1 (MQ=60)
                                                   AAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAG                                                                                                 >  SRR19973070.438358/1‑150 (MQ=60)
                                                   AAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAG                                                                                                 >  SRR19973070.438252/1‑150 (MQ=60)
                                                       CCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTT                                                                                             <  SRR19973070.1201565/150‑1 (MQ=60)
                                                            CAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGT                                                                                        <  SRR19973070.1201426/150‑1 (MQ=60)
                                                              GTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATGAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGT                                                                                      <  SRR19973070.611607/150‑1 (MQ=60)
                                                              GTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGT                                                                                      >  SRR19973070.541498/1‑150 (MQ=60)
                                                               TTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTA                                                                                     >  SRR19973070.982671/1‑150 (MQ=60)
                                                               TTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAGTTTGTTTTGTGCGTGGGACCAGCCTTAAGGTGTA                                                                                     >  SRR19973070.1607911/1‑150 (MQ=60)
                                                                 ACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAA                                                                                   <  SRR19973070.1176106/150‑1 (MQ=60)
                                                                    GCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGG                                                                                <  SRR19973070.248827/150‑1 (MQ=60)
                                                                     CGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGG                                                                               <  SRR19973070.598939/150‑1 (MQ=60)
                                                                     CGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGG                                                                               >  SRR19973070.416788/1‑150 (MQ=60)
                                                                         AAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGG                                                                           <  SRR19973070.1928128/150‑1 (MQ=60)
                                                                         AAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGG                                                                           <  SRR19973070.248045/150‑1 (MQ=60)
                                                                         AAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGG                                                                           <  SRR19973070.1590337/150‑1 (MQ=60)
                                                                         AAACCTCATCCTATGGGAGAACAATGAATAATGAAATGGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGG                                                                           <  SRR19973070.1589961/150‑1 (MQ=60)
                                                                          AACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGT                                                                          <  SRR19973070.3280007/150‑1 (MQ=60)
                                                                          AACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGT                                                                          <  SRR19973070.2368161/150‑1 (MQ=60)
                                                                           ACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTG                                                                         <  SRR19973070.3122860/150‑1 (MQ=60)
                                                                           ACCTCATCCGATGGGAGAACAATGAATAATGAAAGTGCTGGGTTATCATCTCCCAGTATATCCATACTAACGATAATGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGTTGTAAAGGGGGAGGTG                                                                         <  SRR19973070.3122962/150‑1 (MQ=60)
                                                                             CTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGA                                                                       >  SRR19973070.1313465/1‑150 (MQ=60)
                                                                                ATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAAT                                                                    <  SRR19973070.1354650/150‑1 (MQ=60)
                                                                                  CCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCACCCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAG                                                                  <  SRR19973070.1331083/150‑1 (MQ=60)
                                                                                  CCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAG                                                                  <  SRR19973070.2140990/150‑1 (MQ=60)
                                                                                  CCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAG                                                                  <  SRR19973070.1331184/150‑1 (MQ=60)
                                                                                   CTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGC                                                                 >  SRR19973070.3193999/1‑150 (MQ=60)
                                                                                         GAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAG                                                           >  SRR19973070.1625581/1‑150 (MQ=60)
                                                                                          AGAACAATGAATAATGAAATTGCGGTGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGG                                                          <  SRR19973070.2118485/150‑1 (MQ=60)
                                                                                              CAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTA                                                      <  SRR19973070.3090652/150‑1 (MQ=60)
                                                                                               AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     >  SRR19973070.1988620/1‑150 (MQ=60)
                                                                                               AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     >  SRR19973070.330368/1‑150 (MQ=60)
                                                                                               AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     >  SRR19973070.330692/1‑150 (MQ=60)
                                                                                               AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     >  SRR19973070.1937841/1‑150 (MQ=60)
                                                                                               AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     >  SRR19973070.1937741/1‑150 (MQ=60)
                                                                                               AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     >  SRR19973070.1834906/1‑150 (MQ=60)
                                                                                               AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     >  SRR19973070.1710450/1‑150 (MQ=60)
                                                                                                ATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATC                                                    >  SRR19973070.1755014/1‑150 (MQ=60)
                                                                                                ATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATC                                                    >  SRR19973070.2499679/1‑150 (MQ=60)
                                                                                                ATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATC                                                    >  SRR19973070.830240/1‑150 (MQ=60)
                                                                                                  GAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAG                                                  <  SRR19973070.1530735/150‑1 (MQ=60)
                                                                                                  GAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAG                                                  <  SRR19973070.616972/150‑1 (MQ=60)
                                                                                                  GAATAATGAAAGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAG                                                  <  SRR19973070.2289395/150‑1 (MQ=60)
                                                                                                      AATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAG                                              >  SRR19973070.1856722/1‑150 (MQ=60)
                                                                                                         GAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAAT                                           <  SRR19973070.1104636/150‑1 (MQ=60)
                                                                                                            AGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACT                                        <  SRR19973070.2499493/150‑1 (MQ=60)
                                                                                                                CGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCC                                    >  SRR19973070.616456/1‑150 (MQ=60)
                                                                                                                  GGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGC                                  >  SRR19973070.259841/1‑150 (MQ=60)
                                                                                                                   GGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCT                                 <  SRR19973070.3215246/150‑1 (MQ=60)
                                                                                                                     TTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTT                               <  SRR19973070.216897/150‑1 (MQ=60)
                                                                                                                       ATCATCTCCCAGTATATCCATACTAGCGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAC                             >  SRR19973070.3179035/1‑150 (MQ=60)
                                                                                                                        TCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACC                            >  SRR19973070.121331/1‑150 (MQ=60)
                                                                                                                        TCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACC                            >  SRR19973070.121346/1‑150 (MQ=60)
                                                                                                                         CATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCA                           <  SRR19973070.2374917/150‑1 (MQ=60)
                                                                                                                         CATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCA                           >  SRR19973070.2876845/1‑150 (MQ=60)
                                                                                                                         CATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCA                           >  SRR19973070.1437537/1‑150 (MQ=60)
                                                                                                                               CCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGT                     >  SRR19973070.1297900/1‑150 (MQ=60)
                                                                                                                                CAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTG                    >  SRR19973070.211021/1‑150 (MQ=60)
                                                                                                                                   TATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGAT                 <  SRR19973070.2836449/150‑1 (MQ=60)
                                                                                                                                   TATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGAT                 <  SRR19973070.1176403/150‑1 (MQ=60)
                                                                                                                                       TCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGA             <  SRR19973070.1495106/150‑1 (MQ=60)
                                                                                                                                        CCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAG            <  SRR19973070.248825/150‑1 (MQ=60)
                                                                                                                                          ATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGG          >  SRR19973070.3123081/1‑150 (MQ=60)
                                                                                                                                          ATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGG          >  SRR19973070.177927/1‑150 (MQ=60)
                                                                                                                                          ATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGG          >  SRR19973070.1659843/1‑150 (MQ=60)
                                                                                                                                            ACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGAT        <  SRR19973070.984718/150‑1 (MQ=60)
                                                                                                                                              TAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGA      >  SRR19973070.421680/1‑150 (MQ=60)
                                                                                                                                              TAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGA      <  SRR19973070.3202721/150‑1 (MQ=60)
                                                                                                                                                  GATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGTATGAGAGGGATGAAAAA  >  SRR19973070.576221/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  CP009273/1219387‑1219682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑