Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,219,533 A→G 100% intergenic (+170/+202) ycgI → / ← minE pseudogene/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,219,5330AG97.0% 886.6 / 8.1 232intergenic (+170/+202)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base A (2/5);  new base G (103/122);  total (105/127)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 232 total aligned reads displayed.

TCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATG  >  CP009273/1219386‑1219677
                                                                                                                                                   |                                                                                                                                                
tCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGAt                                                                                                                                                >  2:2164916/1‑150 (MQ=255)
 cGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATa                                                                                                                                               <  2:2481371/150‑1 (MQ=255)
   gcgcAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAg                                                                                                                                             <  2:4471491/150‑1 (MQ=255)
    cgcAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAgg                                                                                                                                            <  2:4137340/150‑1 (MQ=255)
      cAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGtt                                                                                                                                          <  2:4273277/150‑1 (MQ=255)
          tttCGATTTCAGTATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGATAACAATGAATAATGAAATTGCGTGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTAttt                                                                                                                                      <  2:3872216/150‑1 (MQ=255)
            tCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTAc                                                                                                                                    <  2:3708263/150‑1 (MQ=255)
             cGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACt                                                                                                                                   <  2:4344759/150‑1 (MQ=255)
              gTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTc                                                                                                                                  <  2:2357933/150‑1 (MQ=255)
               tttACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCa                                                                                                                                 >  1:3431500/1‑150 (MQ=255)
                 tACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAAc                                                                                                                               >  1:421956/1‑150 (MQ=255)
                   cAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCa                                                                                                                             >  2:1096296/1‑150 (MQ=255)
                    aGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAg                                                                                                                            <  2:1892829/150‑1 (MQ=255)
                     ggATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAgg                                                                                                                           <  2:2094710/150‑1 (MQ=255)
                     ggATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAgg                                                                                                                           <  2:1574416/150‑1 (MQ=255)
                     ggATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAgg                                                                                                                           >  2:3077874/1‑150 (MQ=255)
                       aTGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCa                                                                                                                         <  2:2995650/150‑1 (MQ=255)
                          ttGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATaa                                                                                                                      >  1:2505849/1‑150 (MQ=255)
                            gacaGGGAAAATTCTGGGGAAAATAAAGCCCGCCAGTTTCGGCTGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAAc                                                                                                                    <  2:1240769/147‑1 (MQ=255)
                             aaaGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACa                                                                                                                   >  1:2875349/1‑150 (MQ=255)
                              aaGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAt                                                                                                                  <  1:4616579/150‑1 (MQ=255)
                              aaGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAt                                                                                                                  <  1:514194/150‑1 (MQ=255)
                                gtgAAAATTCTGGGGCAAAAAAAGCACGCCAGTTACGGCGGGTAACCTCATCCTATGGGAGATCTTTGAATTATGTAATTGCGGGGTTATCATCTCCCAGTATATCCATACTATCGATAAGGTTATTTACTCAACCAGGCATAAACAttt                                                                                                                <  1:28309/148‑1 (MQ=255)
                                gggAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttt                                                                                                                >  2:2745924/1‑150 (MQ=255)
                                 ggAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAtttt                                                                                                               <  2:4542425/150‑1 (MQ=255)
                                  gAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttttg                                                                                                              <  2:3472472/150‑1 (MQ=255)
                                    aaaTTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttttgtt                                                                                                            >  1:1690400/1‑150 (MQ=255)
                                    aaaTTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttttgtg                                                                                                            >  1:579289/1‑149 (MQ=255)
                                     aaTTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttttgttt                                                                                                           >  1:1460831/1‑150 (MQ=255)
                                      aTTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttttgtttt                                                                                                          >  1:4071127/1‑150 (MQ=255)
                                        tCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATtttgttttgt                                                                                                        <  2:2321686/150‑1 (MQ=255)
                                        tCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATtttgttttgt                                                                                                        <  2:1205165/150‑1 (MQ=255)
                                             ggCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTg                                                                                                   <  1:4447248/150‑1 (MQ=255)
                                                aaaaaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGa                                                                                                >  1:1227715/1‑150 (MQ=255)
                                                  aaaaaGCCCGCCAGTAACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCATTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAAc                                                                                              <  2:2631414/150‑1 (MQ=255)
                                                   aaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACa                                                                                             <  1:32033/150‑1 (MQ=255)
                                                   aaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACa                                                                                             >  2:1148591/1‑150 (MQ=255)
                                                    aaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGACCAg                                                                                            >  1:1862429/1‑150 (MQ=255)
                                                    aaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAg                                                                                            >  1:3154632/1‑150 (MQ=255)
                                                    aaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAg                                                                                            >  2:4072181/1‑150 (MQ=255)
                                                     aaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTACCGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGc                                                                                           >  1:3674041/1‑150 (MQ=255)
                                                     aaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGc                                                                                           <  2:4296047/150‑1 (MQ=255)
                                                        cccGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCtt                                                                                        <  1:3617062/150‑1 (MQ=255)
                                                          cGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTaa                                                                                      <  1:3225622/150‑1 (MQ=255)
                                                          cGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTaa                                                                                      <  2:2853448/150‑1 (MQ=255)
                                                          cGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTaa                                                                                      <  1:533632/150‑1 (MQ=255)
                                                          cGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTaa                                                                                      <  1:858256/150‑1 (MQ=255)
                                                                ttACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTa                                                                                >  2:811800/1‑150 (MQ=255)
                                                                  aCGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTaaa                                                                              >  1:1694348/1‑150 (MQ=255)
                                                                     gcggGAAACCTCATGCTATGGGAGAACATTGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggg                                                                           <  2:1736706/150‑1 (MQ=255)
                                                                      cggGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAgggg                                                                          <  1:4570812/150‑1 (MQ=255)
                                                                       gggAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggggg                                                                         >  2:3633128/1‑150 (MQ=255)
                                                                       gggAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggggg                                                                         >  2:2912578/1‑150 (MQ=255)
                                                                          aaaCCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAgg                                                                      <  1:200207/150‑1 (MQ=255)
                                                                          aaaCCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAgg                                                                      <  2:3693264/150‑1 (MQ=255)
                                                                          aaaCCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAgg                                                                      <  2:2902586/150‑1 (MQ=255)
                                                                           aaCCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGNGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAANGTGTAAAGGGGGAGGt                                                                     <  2:2950727/150‑1 (MQ=255)
                                                                            aCCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTg                                                                    <  2:2005381/150‑1 (MQ=255)
                                                                               tCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGaa                                                                 >  2:1173249/1‑150 (MQ=255)
                                                                                cATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGaaa                                                                >  1:302110/1‑150 (MQ=255)
                                                                                 aTCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAAt                                                               <  1:4556131/150‑1 (MQ=255)
                                                                                  tCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATa                                                              >  2:3147710/1‑150 (MQ=255)
                                                                                    cTATGGGATAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGc                                                            <  1:958453/150‑1 (MQ=255)
                                                                                    cTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGc                                                            <  1:958442/150‑1 (MQ=255)
                                                                                      aTGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCaa                                                          <  1:2873186/150‑1 (MQ=255)
                                                                                       tGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAAt                                                         <  1:2654322/150‑1 (MQ=255)
                                                                                        gggAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATg                                                        <  2:3875422/150‑1 (MQ=255)
                                                                                         ggAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATga                                                       <  2:1972615/150‑1 (MQ=255)
                                                                                           agaACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgagg                                                     <  2:2240921/150‑1 (MQ=255)
                                                                                            gaACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgagga                                                    >  1:2604343/1‑150 (MQ=255)
                                                                                             aaCAATGAATAATGAAAGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgaggag                                                   <  1:108099/150‑1 (MQ=255)
                                                                                               cAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTa                                                 >  2:734542/1‑150 (MQ=255)
                                                                                                 aTGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATc                                               >  1:133664/1‑150 (MQ=255)
                                                                                                  tGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCa                                              >  1:3837331/1‑150 (MQ=255)
                                                                                                   gAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAg                                             <  1:2119911/150‑1 (MQ=255)
                                                                                                    aataatGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATGTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGc                                            >  1:2751507/1‑150 (MQ=255)
                                                                                                     ataatGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCa                                           >  1:1013326/1‑150 (MQ=255)
                                                                                                       aatGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAg                                         <  2:4071127/150‑1 (MQ=255)
                                                                                                           aaaGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATa                                     <  1:1936056/150‑1 (MQ=255)
                                                                                                              ttGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTc                                  <  1:3719757/150‑1 (MQ=255)
                                                                                                                 cGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTcgcc                               <  2:1492804/150‑1 (MQ=255)
                                                                                                                  ggggTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTcgccg                              >  1:2286805/1‑150 (MQ=255)
                                                                                                                        atcatcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAc                        >  2:145514/1‑150 (MQ=255)
                                                                                                                        atcatcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAc                        >  1:4438427/1‑150 (MQ=255)
                                                                                                                        atcatcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAAGGAGGAGTATCAGCAAGAATACTCGCCGCTTTAc                        >  2:4633206/1‑150 (MQ=255)
                                                                                                                        atcatcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTGAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCACGAATACTCGCCGCtttcc                        >  1:3524856/1‑148 (MQ=255)
                                                                                                                         tcatcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTACAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAcc                       >  2:1431353/1‑150 (MQ=255)
                                                                                                                          catcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACca                      >  1:3530860/1‑150 (MQ=255)
                                                                                                                           atcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACcac                     >  1:2420860/1‑150 (MQ=255)
                                                                                                                            tctcCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACcaca                    >  1:3065942/1‑150 (MQ=255)
                                                                                                                              tcCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAAc                  <  1:4072181/150‑1 (MQ=255)
                                                                                                                               cccAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACg                 <  1:2860823/150‑1 (MQ=255)
                                                                                                                                ccAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAGTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGt                >  1:2885442/1‑150 (MQ=255)
                                                                                                                                 cAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTg               >  2:3913901/1‑150 (MQ=255)
                                                                                                                                  aGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTgg              <  1:703080/150‑1 (MQ=255)
                                                                                                                                  aGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTgg              <  2:2413935/150‑1 (MQ=255)
                                                                                                                                    tataTCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGAt            >  2:3423222/1‑150 (MQ=255)
                                                                                                                                       atCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATgag         >  1:3836607/1‑150 (MQ=255)
                                                                                                                                          cATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGAGGAGAgg      >  2:3389223/1‑150 (MQ=255)
                                                                                                                                             aCTAACGTTAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGAt   >  2:663940/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATG  >  CP009273/1219386‑1219677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  CP009273/1219385‑1219682
                                                                                                                                                    |                                                                                                                                                     
TTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGA                                                                                                                                                      >  SRR19973073.3413797/1‑150 (MQ=60)
 TCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGAT                                                                                                                                                     >  SRR19973073.1096549/1‑150 (MQ=60)
  CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATA                                                                                                                                                    >  SRR19973073.2882154/1‑150 (MQ=60)
  CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATA                                                                                                                                                    <  SRR19973073.4572154/150‑1 (MQ=60)
   GGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAA                                                                                                                                                   >  SRR19973073.4555240/1‑150 (MQ=60)
    GCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGG                                                                                                                                                 <  SRR19973073.3071977/150‑1 (MQ=60)
     CGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGG                                                                                                                                                 <  SRR19973073.287725/150‑1 (MQ=60)
              CGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACT                                                                                                                                        <  SRR19973073.4152933/150‑1 (MQ=60)
                TTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCA                                                                                                                                      >  SRR19973073.3438339/1‑150 (MQ=60)
                TTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCA                                                                                                                                      >  SRR19973073.3438355/1‑150 (MQ=60)
                  TACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAAC                                                                                                                                    >  SRR19973073.422803/1‑150 (MQ=60)
                  TACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAAC                                                                                                                                    >  SRR19973073.4331563/1‑150 (MQ=60)
                     AGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAG                                                                                                                                 >  SRR19973073.974164/1‑150 (MQ=60)
                     AGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAAAGATAAGGTTATTTACTCAACCAG                                                                                                                                 >  SRR19973073.974175/1‑150 (MQ=60)
                      GGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTACGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGG                                                                                                                                >  SRR19973073.4384076/1‑150 (MQ=60)
                           TTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAA                                                                                                                           >  SRR19973073.2511034/1‑150 (MQ=60)
                           TTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAA                                                                                                                           >  SRR19973073.2511023/1‑150 (MQ=60)
                              AAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACA                                                                                                                        >  SRR19973073.2881225/1‑150 (MQ=60)
                               AAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAT                                                                                                                       <  SRR19973073.4625792/150‑1 (MQ=60)
                               AAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAT                                                                                                                       <  SRR19973073.515350/150‑1 (MQ=60)
                               AAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAT                                                                                                                       <  SRR19973073.515297/150‑1 (MQ=60)
                               AAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAT                                                                                                                       <  SRR19973073.4625796/150‑1 (MQ=60)
                                AGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATT                                                                                                                      <  SRR19973073.3180458/150‑1 (MQ=60)
                                 GTGAAAATTCTGGGGCAAAAAAAGCACGCCAGTTACGGCGGGTAACCTCATCCTATGGGAGATCTTTGAATTATGTAATTGCGGGGTTATCATCTCCCAGTATATCCATACTATCGATAAGGTTATTTACTCAACCAGGCATAAACATTT                                                                                                                     <  SRR19973073.28390/150‑1 (MQ=60)
                                     AAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTT                                                                                                                 >  SRR19973073.1693907/1‑150 (MQ=60)
                                     AAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTG                                                                                                                 >  SRR19973073.580530/1‑150 (MQ=60)
                                      AATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTT                                                                                                                >  SRR19973073.1463894/1‑150 (MQ=60)
                                      AATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTT                                                                                                                >  SRR19973073.1464033/1‑150 (MQ=60)
                                       ATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTT                                                                                                               >  SRR19973073.4079274/1‑150 (MQ=60)
                                         TCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGT                                                                                                             >  SRR19973073.1747996/1‑150 (MQ=60)
                                              GGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTG                                                                                                        <  SRR19973073.4456179/150‑1 (MQ=60)
                                                 AAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGA                                                                                                     >  SRR19973073.2955120/1‑150 (MQ=60)
                                                 AAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGA                                                                                                     >  SRR19973073.1230281/1‑150 (MQ=60)
                                                    AAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACA                                                                                                  <  SRR19973073.35715/150‑1 (MQ=60)
                                                    AAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACA                                                                                                  <  SRR19973073.32133/150‑1 (MQ=60)
                                                    AAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACA                                                                                                  <  SRR19973073.32126/150‑1 (MQ=60)
                                                    AAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACA                                                                                                  <  SRR19973073.2380644/150‑1 (MQ=60)
                                                     AAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGACCAG                                                                                                 >  SRR19973073.1866245/1‑150 (MQ=60)
                                                     AAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAG                                                                                                 >  SRR19973073.3160987/1‑150 (MQ=60)
                                                     AAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAG                                                                                                 >  SRR19973073.3161009/1‑150 (MQ=60)
                                                     AAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAG                                                                                                 >  SRR19973073.1302628/1‑150 (MQ=60)
                                                      AAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTACCGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGC                                                                                                >  SRR19973073.3681411/1‑150 (MQ=60)
                                                      AAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGC                                                                                                <  SRR19973073.3634096/150‑1 (MQ=60)
                                                         CCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTT                                                                                             <  SRR19973073.3624329/150‑1 (MQ=60)
                                                           CGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAA                                                                                           <  SRR19973073.3232128/150‑1 (MQ=60)
                                                           CGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAA                                                                                           <  SRR19973073.860036/150‑1 (MQ=60)
                                                           CGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAA                                                                                           <  SRR19973073.534786/150‑1 (MQ=60)
                                                           CGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAA                                                                                           <  SRR19973073.534863/150‑1 (MQ=60)
                                                                   ACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAT                                                                                   >  SRR19973073.1697852/1‑150 (MQ=60)
                                                                   ACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAA                                                                                   >  SRR19973073.1697869/1‑150 (MQ=60)
                                                                    CGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAG                                                                                  <  SRR19973073.2970317/150‑1 (MQ=60)
                                                                     GGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGG                                                                                 >  SRR19973073.2278972/1‑150 (MQ=60)
                                                                       CGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGG                                                                               <  SRR19973073.4579953/150‑1 (MQ=60)
                                                                        GGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGG                                                                              >  SRR19973073.952014/1‑150 (MQ=60)
                                                                         GGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGA                                                                             <  SRR19973073.2607525/150‑1 (MQ=60)
                                                                           AAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGG                                                                           <  SRR19973073.392585/150‑1 (MQ=60)
                                                                           AAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGG                                                                           <  SRR19973073.200671/150‑1 (MQ=60)
                                                                             ACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGACCAGCCTTAAGGGGTAAAGGGGGAGGGG                                                                         >  SRR19973073.3682530/1‑150 (MQ=60)
                                                                                TCATACTATGTGAGAACAATGAATAATGAAATTGCGGGTTTATCATCTACCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAA                                                                      <  SRR19973073.2790824/150‑1 (MQ=60)
                                                                                 CATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAA                                                                     >  SRR19973073.302747/1‑150 (MQ=60)
                                                                                  ATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAAT                                                                    <  SRR19973073.4565258/150‑1 (MQ=60)
                                                                                  ATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAAT                                                                    <  SRR19973073.1352109/150‑1 (MQ=60)
                                                                                  ATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAAT                                                                    <  SRR19973073.4565287/150‑1 (MQ=60)
                                                                                   TCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATA                                                                   <  SRR19973073.4594776/150‑1 (MQ=60)
                                                                                     CTATGGGATAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGC                                                                 <  SRR19973073.960454/150‑1 (MQ=60)
                                                                                     CTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGC                                                                 <  SRR19973073.960443/150‑1 (MQ=60)
                                                                                     CTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGC                                                                 <  SRR19973073.960451/150‑1 (MQ=60)
                                                                                      TATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCA                                                                <  SRR19973073.313920/150‑1 (MQ=60)
                                                                                       ATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAA                                                               <  SRR19973073.2879058/150‑1 (MQ=60)
                                                                                       ATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAA                                                               <  SRR19973073.2879329/150‑1 (MQ=60)
                                                                                        TGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAAT                                                              <  SRR19973073.2659762/150‑1 (MQ=60)
                                                                                          GGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGA                                                            >  SRR19973073.2836875/1‑150 (MQ=60)
                                                                                          GGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGA                                                            >  SRR19973073.519674/1‑150 (MQ=60)
                                                                                           GAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAG                                                           <  SRR19973073.783104/150‑1 (MQ=60)
                                                                                             GAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGA                                                         >  SRR19973073.2609709/1‑150 (MQ=60)
                                                                                              AACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAG                                                        <  SRR19973073.3800887/150‑1 (MQ=60)
                                                                                              AACAATGAATAATGAAAGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAG                                                        <  SRR19973073.108366/150‑1 (MQ=60)
                                                                                                  ATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATC                                                    >  SRR19973073.134042/1‑150 (MQ=60)
                                                                                                  ATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATC                                                    >  SRR19973073.133986/1‑150 (MQ=60)
                                                                                                   TGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCA                                                   >  SRR19973073.3480185/1‑150 (MQ=60)
                                                                                                   TGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCA                                                   >  SRR19973073.3844954/1‑150 (MQ=60)
                                                                                                   TGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAAAAGCAATGAGGAGTATCA                                                   >  SRR19973073.3844915/1‑150 (MQ=60)
                                                                                                    GAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAG                                                  <  SRR19973073.2124349/150‑1 (MQ=60)
                                                                                                     AATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGC                                                 <  SRR19973073.2075526/150‑1 (MQ=60)
                                                                                                     AATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGC                                                 <  SRR19973073.2075433/150‑1 (MQ=60)
                                                                                                     AATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATGTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGC                                                 >  SRR19973073.2757144/1‑150 (MQ=60)
                                                                                                      ATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCA                                                >  SRR19973073.1015490/1‑150 (MQ=60)
                                                                                                      ATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCA                                                >  SRR19973073.1015447/1‑150 (MQ=60)
                                                                                                        AATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAG                                              >  SRR19973073.794141/1‑150 (MQ=60)
                                                                                                            AAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATA                                          <  SRR19973073.1940131/150‑1 (MQ=60)
                                                                                                            AAAGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATA                                          <  SRR19973073.1940006/150‑1 (MQ=60)
                                                                                                               TTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTC                                       <  SRR19973073.3727228/150‑1 (MQ=60)
                                                                                                               TTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTC                                       <  SRR19973073.3727224/150‑1 (MQ=60)
                                                                                                                  CGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCC                                    <  SRR19973073.407321/150‑1 (MQ=60)
                                                                                                                   GGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCG                                   >  SRR19973073.2291473/1‑150 (MQ=60)
                                                                                                                        TATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTA                              <  SRR19973073.2666124/150‑1 (MQ=60)
                                                                                                                         ATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAC                             >  SRR19973073.4447339/1‑150 (MQ=60)
                                                                                                                         ATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAC                             >  SRR19973073.4447421/1‑150 (MQ=60)
                                                                                                                         ATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAC                             >  SRR19973073.3531920/1‑150 (MQ=60)
                                                                                                                         ATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAC                             >  SRR19973073.3531926/1‑150 (MQ=60)
                                                                                                                         ATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTGAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCACGAATACTCGCCGCTTTCC                             >  SRR19973073.3531904/1‑150 (MQ=60)
                                                                                                                          TCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACC                            >  SRR19973073.1757773/1‑150 (MQ=60)
                                                                                                                           CATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCA                           >  SRR19973073.3537921/1‑150 (MQ=60)
                                                                                                                            ATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCAC                          >  SRR19973073.2425849/1‑150 (MQ=60)
                                                                                                                            ATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCAC                          >  SRR19973073.2425976/1‑150 (MQ=60)
                                                                                                                             TCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACA                         >  SRR19973073.3072115/1‑150 (MQ=60)
                                                                                                                               TCCCAGTATATCCATACTAACGATAAGGTTATTTACTCCACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAAC                       <  SRR19973073.4080329/150‑1 (MQ=60)
                                                                                                                               TCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAAC                       <  SRR19973073.4080316/150‑1 (MQ=60)
                                                                                                                               TCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAAC                       >  SRR19973073.626623/1‑150 (MQ=60)
                                                                                                                                CCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACG                      <  SRR19973073.2866679/150‑1 (MQ=60)
                                                                                                                                CCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACG                      <  SRR19973073.2866866/150‑1 (MQ=60)
                                                                                                                                 CCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAGTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGT                     >  SRR19973073.2891338/1‑150 (MQ=60)
                                                                                                                                   AGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGG                   <  SRR19973073.704535/150‑1 (MQ=60)
                                                                                                                                        ATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAG              >  SRR19973073.3844229/1‑150 (MQ=60)
                                                                                                                                            ATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGG          <  SRR19973073.1872872/150‑1 (MQ=60)
                                                                                                                                               CTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATG       <  SRR19973073.265201/150‑1 (MQ=60)
                                                                                                                                               CTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATG       <  SRR19973073.275664/150‑1 (MQ=60)
                                                                                                                                                    GATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  SRR19973073.2670706/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  CP009273/1219385‑1219682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑