Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,219,533 A→G 64.1% intergenic (+170/+202) ycgI → / ← minE pseudogene/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,219,5330AG64.1% 250.6 / 240.8 220intergenic (+170/+202)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base A (36/43);  new base G (52/89);  total (88/132)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

CTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  CP009273/1219384‑1219682
                                                                                                                                                     |                                                                                                                                                     
  tCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGAt                                                                                                                                                     <  2:834682/150‑1 (MQ=255)
   cGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATa                                                                                                                                                    <  1:1751127/150‑1 (MQ=255)
      cgcAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAgg                                                                                                                                                 <  2:2781557/150‑1 (MQ=255)
      cgcAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAgg                                                                                                                                                 <  2:1344849/150‑1 (MQ=255)
        cAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGtt                                                                                                                                               <  2:2754580/150‑1 (MQ=255)
          gTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTAt                                                                                                                                             <  2:3418646/150‑1 (MQ=255)
            tttCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTAttt                                                                                                                                           <  1:1412446/150‑1 (MQ=255)
             ttCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTa                                                                                                                                          <  1:3003053/150‑1 (MQ=255)
                   tACAGGATGTTGAAAGTGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAAc                                                                                                                                    <  2:3643247/150‑1 (MQ=255)
                         aTGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCa                                                                                                                              <  1:2092361/150‑1 (MQ=255)
                          tGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCAt                                                                                                                             <  2:148304/150‑1 (MQ=255)
                          tGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCAt                                                                                                                             <  2:4162851/150‑1 (MQ=255)
                           gTTGAGAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATa                                                                                                                            >  2:875001/1‑150 (MQ=255)
                              gAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAAc                                                                                                                         >  1:4359850/1‑150 (MQ=255)
                              gAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAAc                                                                                                                         >  1:3906803/1‑150 (MQ=255)
                               aaaGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACa                                                                                                                        >  2:4188412/1‑150 (MQ=255)
                                aaGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAt                                                                                                                       <  1:2342271/150‑1 (MQ=255)
                                 aGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAtt                                                                                                                      >  2:1178452/1‑150 (MQ=255)
                                  gggAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttt                                                                                                                     <  1:738530/150‑1 (MQ=255)
                                    gAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttttg                                                                                                                   <  2:178570/150‑1 (MQ=255)
                                       aaTTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACAttttgttt                                                                                                                >  2:312766/1‑150 (MQ=255)
                                         ttCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttttgttttg                                                                                                              <  1:2480570/150‑1 (MQ=255)
                                           cTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTtgtg                                                                                                            <  2:3564714/150‑1 (MQ=255)
                                             ggggCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCg                                                                                                          <  1:254145/150‑1 (MQ=255)
                                             ggggCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCg                                                                                                          <  1:2701892/150‑1 (MQ=255)
                                                  aaaaaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGa                                                                                                     >  2:3948592/1‑150 (MQ=255)
                                                  aaaaaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCAATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGa                                                                                                     >  1:265895/1‑150 (MQ=255)
                                                    aaaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAAc                                                                                                   >  1:704304/1‑150 (MQ=255)
                                                     aaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACa                                                                                                  <  1:380878/150‑1 (MQ=255)
                                                     aaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACa                                                                                                  <  2:4869647/150‑1 (MQ=255)
                                                     aaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAAGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGGTAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACa                                                                                                  <  2:3777727/150‑1 (MQ=255)
                                                        aGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGcc                                                                                               >  2:652488/1‑150 (MQ=255)
                                                         gCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCt                                                                                              <  2:1691429/150‑1 (MQ=255)
                                                          cccGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCtt                                                                                             <  2:3225840/150‑1 (MQ=255)
                                                          cccGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCtt                                                                                             <  1:1418791/150‑1 (MQ=255)
                                                           ccGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTa                                                                                            >  2:508653/1‑150 (MQ=255)
                                                            cGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTaa                                                                                           <  2:2020846/150‑1 (MQ=255)
                                                             gCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAg                                                                                          <  2:38998/150‑1 (MQ=255)
                                                              ccAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATACTGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAgg                                                                                         <  2:387381/150‑1 (MQ=255)
                                                              ccAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAgg                                                                                         >  1:4427420/1‑150 (MQ=255)
                                                                aGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGgtg                                                                                       <  2:2995250/150‑1 (MQ=255)
                                                                 gTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGgtgt                                                                                      <  2:922493/150‑1 (MQ=255)
                                                                 gTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGgtgt                                                                                      >  1:3230977/1‑150 (MQ=255)
                                                                  ttACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTa                                                                                     <  2:2169569/150‑1 (MQ=255)
                                                                   tACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTaa                                                                                    >  2:1033994/1‑150 (MQ=255)
                                                                     cggcggGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTACAg                                                                                  >  1:4114278/1‑150 (MQ=255)
                                                                       gcggGAAACCTCATCCTATGGGAGATCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggg                                                                                <  1:1340167/150‑1 (MQ=255)
                                                                       gcggGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggg                                                                                <  2:4640233/150‑1 (MQ=255)
                                                                        cggGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTGAAgggg                                                                               <  2:3733404/150‑1 (MQ=255)
                                                                        cggGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAgggg                                                                               <  1:4810/150‑1 (MQ=255)
                                                                           gAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAg                                                                            >  2:2695619/1‑150 (MQ=255)
                                                                             aaCCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGt                                                                          >  2:4715019/1‑150 (MQ=255)
                                                                             aaCCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCACAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGt                                                                          <  1:1017820/150‑1 (MQ=255)
                                                                               ccTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCGACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTgg                                                                        <  1:4535614/150‑1 (MQ=255)
                                                                               ccTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTgg                                                                        >  2:474844/1‑150 (MQ=255)
                                                                               ccTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTgg                                                                        <  2:445421/150‑1 (MQ=255)
                                                                                cTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGa                                                                       <  1:1877588/150‑1 (MQ=255)
                                                                                 tCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGaa                                                                      <  1:4667347/150‑1 (MQ=255)
                                                                                  cATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGaaa                                                                     <  1:4053709/150‑1 (MQ=255)
                                                                                    tCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATa                                                                   <  2:4294752/150‑1 (MQ=255)
                                                                                    tCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATa                                                                   <  2:4295473/150‑1 (MQ=255)
                                                                                        aTGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCaa                                                               >  2:4340554/1‑150 (MQ=255)
                                                                                               aaCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgaggag                                                        >  2:3818146/1‑150 (MQ=255)
                                                                                                aCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGt                                                       <  2:2086614/150‑1 (MQ=255)
                                                                                                  aaTGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGGGGTGGAAATAGCAATGAGGAGTAt                                                     >  2:1899376/1‑150 (MQ=255)
                                                                                                  aaTGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAt                                                     <  1:499981/150‑1 (MQ=255)
                                                                                                  aaTGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAt                                                     <  1:1772710/150‑1 (MQ=255)
                                                                                                    tGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCa                                                   <  2:440439/150‑1 (MQ=255)
                                                                                                    tGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCa                                                   <  1:2144113/150‑1 (MQ=255)
                                                                                                     gAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAg                                                  <  2:2836681/150‑1 (MQ=255)
                                                                                                      aataatGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGc                                                 <  1:1470463/150‑1 (MQ=255)
                                                                                                       ataatGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTACAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCa                                                >  2:310162/1‑150 (MQ=255)
                                                                                                       ataatGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCa                                                >  1:904447/1‑150 (MQ=255)
                                                                                                        taatGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCaa                                               <  2:2398690/150‑1 (MQ=255)
                                                                                                         aatGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAg                                              <  1:3103981/150‑1 (MQ=255)
                                                                                                         aatGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAg                                              >  2:1540434/1‑150 (MQ=255)
                                                                                                            gAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAAt                                           >  1:3161181/1‑150 (MQ=255)
                                                                                                             aaaTTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATa                                          >  1:2862047/1‑150 (MQ=255)
                                                                                                              aaTTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATAc                                         >  1:3514892/1‑150 (MQ=255)
                                                                                                               aTTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACt                                        >  2:611706/1‑150 (MQ=255)
                                                                                                               aTTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACt                                        <  1:3530446/150‑1 (MQ=255)
                                                                                                                ttGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGACCAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCACGAATACTc                                       >  2:4574698/1‑150 (MQ=255)
                                                                                                                     gggTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTcgccgc                                  >  1:876407/1‑150 (MQ=255)
                                                                                                                        ttATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCttt                               >  2:432442/1‑150 (MQ=255)
                                                                                                                        ttATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCttt                               >  2:3386152/1‑150 (MQ=255)
                                                                                                                         tATCATCTCCCAGTATATCCATACTAACAATACGGTTATTTACTCACCCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTa                              <  2:1115818/150‑1 (MQ=255)
                                                                                                                          atcatcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAc                             >  2:3603620/1‑150 (MQ=255)
                                                                                                                          atcatcTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAA‑GGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAcc                            >  2:3533774/1‑150 (MQ=255)
                                                                                                                            catcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACca                           >  2:4099589/1‑150 (MQ=255)
                                                                                                                             atcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTCAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACcac                          >  1:1793751/1‑150 (MQ=255)
                                                                                                                               ctcCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACaa                        <  1:2922315/150‑1 (MQ=255)
                                                                                                                                   cAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTg                    >  1:1600898/1‑150 (MQ=255)
                                                                                                                                    aGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTgg                   <  1:2695619/150‑1 (MQ=255)
                                                                                                                                      tataTCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGAt                 <  1:3712162/150‑1 (MQ=255)
                                                                                                                                      tataTCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGAt                 >  1:593375/1‑150 (MQ=255)
                                                                                                                                         atCAATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATgag              >  1:1214783/1‑150 (MQ=255)
                                                                                                                                            cATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAgg           >  1:4695045/1‑150 (MQ=255)
                                                                                                                                               aCTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGAt        <  2:3068016/150‑1 (MQ=255)
                                                                                                                                                cTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATg       <  1:2284392/150‑1 (MQ=255)
                                                                                                                                                     aaTAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGaaaaa  >  1:622478/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  CP009273/1219384‑1219682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  CP009273/1219384‑1219682
                                                                                                                                                     |                                                                                                                                                     
CTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACA                                                                                                                                                       <  SRR19973081.319623/150‑1 (MQ=60)
 TTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGA                                                                                                                                                      <  SRR19973081.1615228/150‑1 (MQ=60)
 TTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAAGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGA                                                                                                                                                      <  SRR19973081.1616436/150‑1 (MQ=60)
   CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATA                                                                                                                                                    >  SRR19973081.1363158/1‑150 (MQ=60)
   CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATA                                                                                                                                                    <  SRR19973081.1762606/150‑1 (MQ=60)
   CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATA                                                                                                                                                    <  SRR19973081.1762790/150‑1 (MQ=60)
      CGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGG                                                                                                                                                 >  SRR19973081.39461/1‑150 (MQ=60)
      CGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGG                                                                                                                                                 <  SRR19973081.2232821/150‑1 (MQ=60)
          GTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTAT                                                                                                                                             <  SRR19973081.1267926/150‑1 (MQ=60)
          GTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTAT                                                                                                                                             <  SRR19973081.1992270/150‑1 (MQ=60)
            TTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTT                                                                                                                                           <  SRR19973081.1421907/150‑1 (MQ=60)
             TTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTA                                                                                                                                          <  SRR19973081.3021560/150‑1 (MQ=60)
                  TTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTGTGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAA                                                                                                                                     <  SRR19973081.144175/150‑1 (MQ=60)
                  TTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTGTGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAA                                                                                                                                     <  SRR19973081.143990/150‑1 (MQ=60)
                      AGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAG                                                                                                                                 <  SRR19973081.3315683/150‑1 (MQ=60)
                         ATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCA                                                                                                                              <  SRR19973081.2105665/150‑1 (MQ=60)
                            TTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAA                                                                                                                           <  SRR19973081.322476/150‑1 (MQ=60)
                             TGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAA                                                                                                                          >  SRR19973081.3086876/1‑150 (MQ=60)
                              GAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAAC                                                                                                                         >  SRR19973081.4386259/1‑150 (MQ=60)
                              GAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAAC                                                                                                                         >  SRR19973081.3930792/1‑150 (MQ=60)
                                AAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAT                                                                                                                       <  SRR19973081.2356874/150‑1 (MQ=60)
                                AAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAT                                                                                                                       <  SRR19973081.2356584/150‑1 (MQ=60)
                                  GGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTT                                                                                                                     <  SRR19973081.743365/150‑1 (MQ=60)
                                  GGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTT                                                                                                                     >  SRR19973081.2696443/1‑150 (MQ=60)
                                        ATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTT                                                                                                               >  SRR19973081.4651449/1‑150 (MQ=60)
                                         TTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTG                                                                                                              <  SRR19973081.2495991/150‑1 (MQ=60)
                                         TTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTG                                                                                                              <  SRR19973081.2496076/150‑1 (MQ=60)
                                             GGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCG                                                                                                          <  SRR19973081.2718616/150‑1 (MQ=60)
                                             GGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCG                                                                                                          <  SRR19973081.256210/150‑1 (MQ=60)
                                             GGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCG                                                                                                          <  SRR19973081.2718323/150‑1 (MQ=60)
                                                  AAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGA                                                                                                     >  SRR19973081.557718/1‑150 (MQ=60)
                                                  AAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCAATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGA                                                                                                     >  SRR19973081.268017/1‑150 (MQ=60)
                                                   AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAA                                                                                                    >  SRR19973081.1424463/1‑150 (MQ=60)
                                                    AAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAAC                                                                                                   >  SRR19973081.709000/1‑150 (MQ=60)
                                                     AAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACA                                                                                                  <  SRR19973081.383378/150‑1 (MQ=60)
                                                     AAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACA                                                                                                  >  SRR19973081.2236867/1‑150 (MQ=60)
                                                     AAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACA                                                                                                  >  SRR19973081.1618238/1‑150 (MQ=60)
                                                       AAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGC                                                                                                >  SRR19973081.658404/1‑150 (MQ=60)
                                                        AGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCC                                                                                               <  SRR19973081.75446/150‑1 (MQ=60)
                                                        AGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCC                                                                                               >  SRR19973081.724957/1‑150 (MQ=60)
                                                          CCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTT                                                                                             <  SRR19973081.2121762/150‑1 (MQ=60)
                                                          CCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTT                                                                                             <  SRR19973081.1428294/150‑1 (MQ=60)
                                                          CCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTT                                                                                             <  SRR19973081.3903780/150‑1 (MQ=60)
                                                              CCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGG                                                                                         >  SRR19973081.4454007/1‑150 (MQ=60)
                                                              CCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGG                                                                                         >  SRR19973081.4454061/1‑150 (MQ=60)
                                                               CAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGT                                                                                        >  SRR19973081.4434713/1‑150 (MQ=60)
                                                                AGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTG                                                                                       <  SRR19973081.653596/150‑1 (MQ=60)
                                                                 GTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGT                                                                                      >  SRR19973081.3250453/1‑150 (MQ=60)
                                                                     CGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGACCAGCCTTAAGGTGTAAAG                                                                                  >  SRR19973081.4139350/1‑150 (MQ=60)
                                                                     CGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTACAG                                                                                  >  SRR19973081.4139331/1‑150 (MQ=60)
                                                                       GCGGGAAACCTCATCCTATGGGAGATCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGG                                                                                <  SRR19973081.1349139/150‑1 (MQ=60)
                                                                        CGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGG                                                                               <  SRR19973081.4870/150‑1 (MQ=60)
                                                                           GAAACCTCATCCGATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAG                                                                            <  SRR19973081.994820/150‑1 (MQ=60)
                                                                            AAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGG                                                                           >  SRR19973081.4892538/1‑150 (MQ=60)
                                                                             AACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGT                                                                          <  SRR19973081.4130683/150‑1 (MQ=60)
                                                                             AACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCACAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGT                                                                          <  SRR19973081.1024702/150‑1 (MQ=60)
                                                                               CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCGACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGG                                                                        <  SRR19973081.4563099/150‑1 (MQ=60)
                                                                                CTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGA                                                                       <  SRR19973081.1889681/150‑1 (MQ=60)
                                                                                CTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGA                                                                       <  SRR19973081.4743512/150‑1 (MQ=60)
                                                                                CTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGA                                                                       <  SRR19973081.4743516/150‑1 (MQ=60)
                                                                                 TCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAA                                                                      <  SRR19973081.963692/150‑1 (MQ=60)
                                                                                 TCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAA                                                                      <  SRR19973081.4695671/150‑1 (MQ=60)
                                                                                  CATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAA                                                                     <  SRR19973081.4078178/150‑1 (MQ=60)
                                                                                       TATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCA                                                                >  SRR19973081.2609678/1‑150 (MQ=60)
                                                                                       TATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCA                                                                >  SRR19973081.2613772/1‑150 (MQ=60)
                                                                                       TATGGGAGAACAAGGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCA                                                                <  SRR19973081.2460733/150‑1 (MQ=60)
                                                                                                  AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     <  SRR19973081.3712015/150‑1 (MQ=60)
                                                                                                  AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     <  SRR19973081.503552/150‑1 (MQ=60)
                                                                                                  AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     <  SRR19973081.1784314/150‑1 (MQ=60)
                                                                                                  AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     <  SRR19973081.1784308/150‑1 (MQ=60)
                                                                                                  AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     >  SRR19973081.3018972/1‑150 (MQ=60)
                                                                                                    TGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCA                                                   <  SRR19973081.2157783/150‑1 (MQ=60)
                                                                                                      AATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGC                                                 <  SRR19973081.1480217/150‑1 (MQ=60)
                                                                                                       ATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCA                                                >  SRR19973081.910529/1‑150 (MQ=60)
                                                                                                         AATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAG                                              <  SRR19973081.3123002/150‑1 (MQ=60)
                                                                                                            GAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAAT                                           >  SRR19973081.3179815/1‑150 (MQ=60)
                                                                                                            GAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAAT                                           >  SRR19973081.3180445/1‑150 (MQ=60)
                                                                                                             AAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATA                                          >  SRR19973081.2879566/1‑150 (MQ=60)
                                                                                                             AAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATA                                          >  SRR19973081.2879510/1‑150 (MQ=60)
                                                                                                              AATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATAC                                         >  SRR19973081.3536408/1‑150 (MQ=60)
                                                                                                               ATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACT                                        <  SRR19973081.3551776/150‑1 (MQ=60)
                                                                                                               ATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACT                                        <  SRR19973081.3552024/150‑1 (MQ=60)
                                                                                                                TTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTC                                       <  SRR19973081.4058929/150‑1 (MQ=60)
                                                                                                                     GGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGC                                  >  SRR19973081.882214/1‑150 (MQ=60)
                                                                                                                       GTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTT                                >  SRR19973081.2729644/1‑150 (MQ=60)
                                                                                                                             ATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTCAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCAC                          >  SRR19973081.1805477/1‑150 (MQ=60)
                                                                                                                             ATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCAC                          >  SRR19973081.1804973/1‑150 (MQ=60)
                                                                                                                               CTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAA                        <  SRR19973081.4554428/150‑1 (MQ=60)
                                                                                                                               CTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAA                        <  SRR19973081.2940335/150‑1 (MQ=60)
                                                                                                                               CGCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAA                        <  SRR19973081.2940319/150‑1 (MQ=60)
                                                                                                                                   CAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTCAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTG                    >  SRR19973081.1061790/1‑150 (MQ=60)
                                                                                                                                   CAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTG                    >  SRR19973081.1611153/1‑150 (MQ=60)
                                                                                                                                    AGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGG                   <  SRR19973081.2712317/150‑1 (MQ=60)
                                                                                                                                      TATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGAT                 >  SRR19973081.597555/1‑150 (MQ=60)
                                                                                                                                      TATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGAT                 <  SRR19973081.3734797/150‑1 (MQ=60)
                                                                                                                                      TATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGAT                 <  SRR19973081.3734826/150‑1 (MQ=60)
                                                                                                                                       ATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATG                >  SRR19973081.1515602/1‑150 (MQ=60)
                                                                                                                                         ATCAATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAG              >  SRR19973081.1222663/1‑150 (MQ=60)
                                                                                                                                           CCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAG            <  SRR19973081.1851694/150‑1 (MQ=60)
                                                                                                                                            CATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGG           >  SRR19973081.4723228/1‑150 (MQ=60)
                                                                                                                                            CATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGG           >  SRR19973081.4723510/1‑150 (MQ=60)
                                                                                                                                                CTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATG       <  SRR19973081.2298770/150‑1 (MQ=60)
                                                                                                                                                     AATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  SRR19973081.626821/1‑150 (MQ=60)
                                                                                                                                                     AATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  SRR19973081.907301/1‑150 (MQ=60)
                                                                                                                                                     AATAAGGTTATTTACTCAACCAGGCATAAACATATTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACAACAACGTGGATGAGAGGGATGAAAAA  >  SRR19973081.626742/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  CP009273/1219384‑1219682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑