Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,219,533 A→G 100% intergenic (+170/+202) ycgI → / ← minE pseudogene/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,219,5330AG100.0% 919.4 / NA 236intergenic (+170/+202)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (115/121);  total (115/121)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 236 total aligned reads displayed.

CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAA  >  CP009273/1219387‑1219679
                                                                                                                                                  |                                                                                                                                                  
cgtgtcAGTTTTCTTTTACCGGTTGTTGTATGGGATAAATCTGGGGCAACAATAGCCCGCCAGTTACGTCGGGAACCCTCATCCTATGGGAGAACAATGAATAATGAAAGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATa                                                                                                                                                 <  2:5083900/145‑1 (MQ=255)
 ggCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATaa                                                                                                                                                >  1:1791029/1‑150 (MQ=255)
 ggCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATaa                                                                                                                                                <  1:4688288/150‑1 (MQ=255)
       gTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTAt                                                                                                                                          <  2:1953404/150‑1 (MQ=255)
       gTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTAt                                                                                                                                          <  2:4971820/150‑1 (MQ=255)
         tttCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTAttt                                                                                                                                        <  1:3678955/150‑1 (MQ=255)
           tCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTAc                                                                                                                                      <  1:1088193/150‑1 (MQ=255)
             gTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTc                                                                                                                                    >  2:237030/1‑150 (MQ=255)
                tACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAAc                                                                                                                                 >  2:3878410/1‑150 (MQ=255)
                tACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAAc                                                                                                                                 >  2:3769619/1‑150 (MQ=255)
                 accggAGGTTGAAAGTGACAAGTCTGGGGCAAAAAAAGCCCGCCAGTTACGGAGGGAAACCTCATACTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATATCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAccc                                                                                                                                <  2:46489/147‑1 (MQ=255)
                  cAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCa                                                                                                                               <  1:4941108/150‑1 (MQ=255)
                  cAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCa                                                                                                                               <  1:624206/150‑1 (MQ=255)
                  cAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCa                                                                                                                               <  2:2161896/150‑1 (MQ=255)
                   aGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAg                                                                                                                              <  1:1010353/150‑1 (MQ=255)
                   aGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAg                                                                                                                              >  1:1957142/1‑150 (MQ=255)
                   aGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAg                                                                                                                              >  1:317125/1‑150 (MQ=255)
                    ggATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAgg                                                                                                                             >  1:4083092/1‑150 (MQ=255)
                    ggATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAgg                                                                                                                             <  1:1263007/150‑1 (MQ=255)
                         ttGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCGTACTAACGATAAGGTTATTTACTCAACCAGGCATaa                                                                                                                        <  2:1141553/150‑1 (MQ=255)
                          tGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATaaa                                                                                                                       <  2:3798825/150‑1 (MQ=255)
                            aaaGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACa                                                                                                                     >  1:1106770/1‑150 (MQ=255)
                              aGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAtt                                                                                                                   <  1:2667719/150‑1 (MQ=255)
                              aGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAtt                                                                                                                   <  1:5155154/150‑1 (MQ=255)
                              aGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAtt                                                                                                                   >  1:3588187/1‑150 (MQ=255)
                                     aTTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAttttgtttt                                                                                                            >  2:3216616/1‑150 (MQ=255)
                                      ttCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTGCTCAACCAGGCATAAACAttttgttttg                                                                                                           <  2:940454/150‑1 (MQ=255)
                                         tGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGc                                                                                                        >  2:4734336/1‑150 (MQ=255)
                                         tGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGc                                                                                                        >  1:327717/1‑150 (MQ=255)
                                          ggggCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCg                                                                                                       <  1:4656769/150‑1 (MQ=255)
                                            ggCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTg                                                                                                     <  2:3334513/150‑1 (MQ=255)
                                              cAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTggg                                                                                                   >  1:3109697/1‑150 (MQ=255)
                                              cAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTggg                                                                                                   <  2:4437800/150‑1 (MQ=255)
                                               aaaaaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGa                                                                                                  <  1:4019719/150‑1 (MQ=255)
                                               aaaaaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGa                                                                                                  <  1:2813702/150‑1 (MQ=255)
                                               aaaaaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGa                                                                                                  >  2:4000533/1‑150 (MQ=255)
                                               aaaaaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGa                                                                                                  <  1:4861456/150‑1 (MQ=255)
                                                taaaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTAATCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGac                                                                                                 >  1:657609/2‑149 (MQ=255)
                                                aaaaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGaa                                                                                                 >  1:864512/1‑150 (MQ=255)
                                                 aaaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAAc                                                                                                >  1:45644/1‑150 (MQ=255)
                                                  aaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGACCa                                                                                               >  1:4139752/1‑150 (MQ=255)
                                                  aaaaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACa                                                                                               <  1:1565910/150‑1 (MQ=255)
                                                    aaGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGc                                                                                             >  2:492869/1‑150 (MQ=255)
                                                     aGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGcc                                                                                            <  2:3082528/150‑1 (MQ=255)
                                                      gCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCt                                                                                           >  1:137045/1‑150 (MQ=255)
                                                       cccGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAGTTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAGTTTGTTTTGTGCGTGGGAACAGCCtt                                                                                          >  1:2128656/1‑150 (MQ=255)
                                                            cAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGgt                                                                                     >  2:2335581/1‑150 (MQ=255)
                                                            cAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGgt                                                                                     >  1:972617/1‑150 (MQ=255)
                                                            cAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACAAGGCATAAACATTTTGTTTTGTGCGTGGGAACTGCCTTAAGgt                                                                                     >  2:2335357/1‑150 (MQ=255)
                                                             aGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGgtg                                                                                    >  2:3457690/1‑150 (MQ=255)
                                                             aGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGgtg                                                                                    >  2:4933455/1‑150 (MQ=255)
                                                              gTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGgtgt                                                                                   >  1:3823099/1‑150 (MQ=255)
                                                               ttACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTa                                                                                  >  2:2255231/1‑150 (MQ=255)
                                                               ttACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTa                                                                                  <  2:4237201/150‑1 (MQ=255)
                                                               ttACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGAGGGAACAGCCTTAAGGTGTa                                                                                  <  1:839306/150‑1 (MQ=255)
                                                                tACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTaa                                                                                 >  2:2432366/1‑150 (MQ=255)
                                                                tACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTaa                                                                                 >  2:2608206/1‑150 (MQ=255)
                                                                 aCGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAAGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTaaa                                                                                <  2:2243633/150‑1 (MQ=255)
                                                                    gcggGAAACCTCATACTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggg                                                                             <  2:4725622/150‑1 (MQ=255)
                                                                      gggAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggggg                                                                           >  2:1271121/1‑150 (MQ=255)
                                                                      gggAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggggg                                                                           >  1:5030973/1‑150 (MQ=255)
                                                                      gggAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggggg                                                                           >  1:1470041/1‑150 (MQ=255)
                                                                      gggAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAggggg                                                                           >  1:1255825/1‑150 (MQ=255)
                                                                        gAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAg                                                                         <  1:711408/150‑1 (MQ=255)
                                                                            ccTCATCCTATGGGAGAACAATGAATAATGAAATTGTGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTGAAGGTGTAAAGGGGGAGGTgg                                                                     >  2:1181165/1‑150 (MQ=255)
                                                                            ccTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTgg                                                                     <  1:1608600/150‑1 (MQ=255)
                                                                            ccTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTgg                                                                     >  1:995871/1‑150 (MQ=255)
                                                                             cTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGa                                                                    <  1:4800506/150‑1 (MQ=255)
                                                                              tCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGaa                                                                   <  2:388405/150‑1 (MQ=255)
                                                                                  ccTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAg                                                               <  2:815675/150‑1 (MQ=255)
                                                                                   cTATGGGAGAACAATGAATACTGAAAGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGc                                                              <  1:3864411/150‑1 (MQ=255)
                                                                                    tATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCa                                                             <  1:936760/150‑1 (MQ=255)
                                                                                          agaACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgagg                                                       <  1:3058135/150‑1 (MQ=255)
                                                                                            aaCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATgaggag                                                     <  2:3536524/150‑1 (MQ=255)
                                                                                             aCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGt                                                    >  2:679626/1‑150 (MQ=255)
                                                                                             aCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGt                                                    <  2:1744222/150‑1 (MQ=255)
                                                                                                aTGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATc                                                 >  1:1930134/1‑150 (MQ=255)
                                                                                                 tGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCa                                                >  2:7729/1‑150 (MQ=255)
                                                                                                        tGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAGTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCCGCAAGaa                                         >  1:3021756/1‑150 (MQ=255)
                                                                                                            aTTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTCTCAGCAAGAATACt                                     >  2:4103841/1‑150 (MQ=255)
                                                                                                             ttGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTc                                    <  2:4783692/150‑1 (MQ=255)
                                                                                                              tGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTcg                                   >  2:3179257/1‑150 (MQ=255)
                                                                                                                cGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTcgcc                                 >  2:4367198/1‑150 (MQ=255)
                                                                                                                      tATCATCTCCCAGCATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTa                           >  2:269151/1‑150 (MQ=255)
                                                                                                                       atcatcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAc                          >  1:3771541/1‑150 (MQ=255)
                                                                                                                       atcatcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAc                          >  2:1800193/1‑150 (MQ=255)
                                                                                                                         catcTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACca                        >  1:1872661/1‑150 (MQ=255)
                                                                                                                           tctcCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACcaca                      <  1:3216616/150‑1 (MQ=255)
                                                                                                                            ctcCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGACCAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACaa                     >  2:4411550/1‑150 (MQ=255)
                                                                                                                            ctcCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACaa                     >  2:2508954/1‑150 (MQ=255)
                                                                                                                             tcCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAAc                    >  2:1909044/1‑150 (MQ=255)
                                                                                                                             tcCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAAc                    <  2:2877588/150‑1 (MQ=255)
                                                                                                                               ccAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGt                  >  1:75479/1‑150 (MQ=255)
                                                                                                                                     tatCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATga            <  2:137060/150‑1 (MQ=255)
                                                                                                                                      atCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATgag           <  1:2358630/150‑1 (MQ=255)
                                                                                                                                       tCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATgaga          <  2:1586114/150‑1 (MQ=255)
                                                                                                                                        ccATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGTGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGagag         >  2:1608899/1‑150 (MQ=255)
                                                                                                                                        ccATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGagag         >  2:1068872/1‑150 (MQ=255)
                                                                                                                                              tAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGa   <  2:3584399/150‑1 (MQ=255)
                                                                                                                                               aaCGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGaa  >  2:4504750/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAA  >  CP009273/1219387‑1219679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  CP009273/1219387‑1219682
                                                                                                                                                  |                                                                                                                                                     
CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATA                                                                                                                                                    <  SRR19973063.2883260/150‑1 (MQ=60)
CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATA                                                                                                                                                    >  SRR19973063.3155743/1‑150 (MQ=60)
CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATA                                                                                                                                                    >  SRR19973063.3155675/1‑150 (MQ=60)
 GGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAA                                                                                                                                                   >  SRR19973063.1796572/1‑150 (MQ=60)
 GGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAA                                                                                                                                                   <  SRR19973063.1161378/150‑1 (MQ=60)
 GGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAA                                                                                                                                                   <  SRR19973063.1161388/150‑1 (MQ=60)
 GGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAA                                                                                                                                                   <  SRR19973063.4703377/150‑1 (MQ=60)
 GGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAA                                                                                                                                                   <  SRR19973063.4703233/150‑1 (MQ=60)
   CGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGG                                                                                                                                                 <  SRR19973063.588296/150‑1 (MQ=60)
    GCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGT                                                                                                                                                <  SRR19973063.3654197/150‑1 (MQ=60)
      AGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTA                                                                                                                                              <  SRR19973063.2655830/150‑1 (MQ=60)
       GTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTAT                                                                                                                                             <  SRR19973063.3441662/150‑1 (MQ=60)
         TTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTT                                                                                                                                           <  SRR19973063.3690731/150‑1 (MQ=60)
           TCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTAC                                                                                                                                         <  SRR19973063.1091501/150‑1 (MQ=60)
                  CAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCA                                                                                                                                  <  SRR19973063.1324901/150‑1 (MQ=60)
                  CAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCA                                                                                                                                  <  SRR19973063.4957085/150‑1 (MQ=60)
                  CAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCA                                                                                                                                  <  SRR19973063.626040/150‑1 (MQ=60)
                   AGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAG                                                                                                                                 >  SRR19973063.318053/1‑150 (MQ=60)
                   AGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAG                                                                                                                                 <  SRR19973063.1013395/150‑1 (MQ=60)
                   AGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAG                                                                                                                                 >  SRR19973063.1963268/1‑150 (MQ=60)
                   AGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAG                                                                                                                                 <  SRR19973063.2271918/150‑1 (MQ=60)
                    GGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGG                                                                                                                                >  SRR19973063.4096166/1‑150 (MQ=60)
                    GGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGG                                                                                                                                <  SRR19973063.1266764/150‑1 (MQ=60)
                      ATGTTGATAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCA                                                                                                                              <  SRR19973063.184519/150‑1 (MQ=60)
                         TTGAAAGGGAAAATTATTGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAA                                                                                                                           >  SRR19973063.2326572/1‑150 (MQ=60)
                            AAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACA                                                                                                                        >  SRR19973063.1110138/1‑150 (MQ=60)
                              AGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATT                                                                                                                      >  SRR19973063.3599691/1‑150 (MQ=60)
                              AGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATT                                                                                                                      <  SRR19973063.2676284/150‑1 (MQ=60)
                              AGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATT                                                                                                                      <  SRR19973063.5171738/150‑1 (MQ=60)
                                 GAAAATTCGGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCGATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGAATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTG                                                                                                                   <  SRR19973063.3911248/150‑1 (MQ=60)
                                        CTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAGTTTGTTTTGTG                                                                                                            >  SRR19973063.4057724/1‑150 (MQ=60)
                                         TGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGC                                                                                                           >  SRR19973063.328669/1‑150 (MQ=60)
                                         TGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGC                                                                                                           <  SRR19973063.1085332/150‑1 (MQ=60)
                                          GGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCG                                                                                                          <  SRR19973063.4671632/150‑1 (MQ=60)
                                           TGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATGTTGTTTTGTGCGT                                                                                                         >  SRR19973063.1523359/1‑150 (MQ=60)
                                           GGGCAAAAAAAGCCCGCCAGTTTCGGCGGGAAACCTCAGCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGT                                                                                                         <  SRR19973063.3187536/150‑1 (MQ=60)
                                           GGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGT                                                                                                         <  SRR19973063.1587784/150‑1 (MQ=60)
                                              CAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGG                                                                                                      >  SRR19973063.3119704/1‑150 (MQ=60)
                                               AAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGA                                                                                                     <  SRR19973063.4877115/150‑1 (MQ=60)
                                               AAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGA                                                                                                     <  SRR19973063.4661226/150‑1 (MQ=60)
                                               AAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGA                                                                                                     <  SRR19973063.4032614/150‑1 (MQ=60)
                                               AAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGA                                                                                                     <  SRR19973063.4032476/150‑1 (MQ=60)
                                               AAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGA                                                                                                     <  SRR19973063.2822749/150‑1 (MQ=60)
                                                TAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTAATCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAC                                                                                                    >  SRR19973063.659536/1‑150 (MQ=60)
                                                AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAA                                                                                                    >  SRR19973063.867001/1‑150 (MQ=60)
                                                 AAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAAC                                                                                                   >  SRR19973063.45810/1‑150 (MQ=60)
                                                  AAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGACCA                                                                                                  >  SRR19973063.4152993/1‑150 (MQ=60)
                                                  AAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACA                                                                                                  <  SRR19973063.1570718/150‑1 (MQ=60)
                                                     AGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCC                                                                                               <  SRR19973063.4988279/150‑1 (MQ=60)
                                                      GCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCT                                                                                              >  SRR19973063.137477/1‑150 (MQ=60)
                                                      GCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCT                                                                                              >  SRR19973063.137492/1‑150 (MQ=60)
                                                       CCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAGTTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAGTTTGTTTTGTGCGTGGGAACAGCCTT                                                                                             >  SRR19973063.2135304/1‑150 (MQ=60)
                                                         CGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAA                                                                                           <  SRR19973063.1956869/150‑1 (MQ=60)
                                                            CAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGT                                                                                        >  SRR19973063.975551/1‑150 (MQ=60)
                                                            CAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGT                                                                                        >  SRR19973063.3691152/1‑150 (MQ=60)
                                                             AGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTATGGTG                                                                                       >  SRR19973063.936931/1‑150 (MQ=60)
                                                             AGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTG                                                                                       >  SRR19973063.3048744/1‑150 (MQ=60)
                                                             AGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTG                                                                                       >  SRR19973063.3859983/1‑150 (MQ=60)
                                                              GTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGT                                                                                      >  SRR19973063.1096804/1‑150 (MQ=60)
                                                              GTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGT                                                                                      >  SRR19973063.3835270/1‑150 (MQ=60)
                                                               TTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTA                                                                                     <  SRR19973063.3103094/150‑1 (MQ=60)
                                                               TTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGAGGGAACAGCCTTAAGGTGTA                                                                                     <  SRR19973063.841733/150‑1 (MQ=60)
                                                                   GGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGG                                                                                 <  SRR19973063.3268802/150‑1 (MQ=60)
                                                                    GCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGG                                                                                <  SRR19973063.2823855/150‑1 (MQ=60)
                                                                      GGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGG                                                                              >  SRR19973063.1474535/1‑150 (MQ=60)
                                                                      GGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGG                                                                              >  SRR19973063.1259562/1‑150 (MQ=60)
                                                                      GGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGG                                                                              >  SRR19973063.5047194/1‑150 (MQ=60)
                                                                       GGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGA                                                                             >  SRR19973063.1668985/1‑150 (MQ=60)
                                                                        GAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAG                                                                            <  SRR19973063.713498/150‑1 (MQ=60)
                                                                          AACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTACCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGT                                                                          >  SRR19973063.4563482/1‑150 (MQ=60)
                                                                            CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGG                                                                        <  SRR19973063.1613523/150‑1 (MQ=60)
                                                                            CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGG                                                                        >  SRR19973063.998690/1‑150 (MQ=60)
                                                                            CCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGG                                                                        >  SRR19973063.998875/1‑150 (MQ=60)
                                                                             CTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGA                                                                       <  SRR19973063.4815848/150‑1 (MQ=60)
                                                                             CTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGA                                                                       >  SRR19973063.4279401/1‑150 (MQ=60)
                                                                               CATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAA                                                                     <  SRR19973063.1879384/150‑1 (MQ=60)
                                                                                 TCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATA                                                                   <  SRR19973063.5086491/150‑1 (MQ=60)
                                                                                  CCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAG                                                                  <  SRR19973063.707816/150‑1 (MQ=60)
                                                                                   CTATGGGAGAACAATGAATACTGAAAGTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGC                                                                 <  SRR19973063.3876702/150‑1 (MQ=60)
                                                                                    TATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCA                                                                <  SRR19973063.939644/150‑1 (MQ=60)
                                                                                    TATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCA                                                                <  SRR19973063.939553/150‑1 (MQ=60)
                                                                                       GGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATG                                                             >  SRR19973063.540621/1‑150 (MQ=60)
                                                                                       GGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATG                                                             <  SRR19973063.138093/150‑1 (MQ=60)
                                                                                        GGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGA                                                            >  SRR19973063.1690858/1‑150 (MQ=60)
                                                                                        GGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGA                                                            <  SRR19973063.745865/150‑1 (MQ=60)
                                                                                        CGAGAACACTGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGA                                                            <  SRR19973063.745803/150‑1 (MQ=60)
                                                                                          AGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGG                                                          <  SRR19973063.3067941/150‑1 (MQ=60)
                                                                                           GAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGA                                                         <  SRR19973063.4924297/150‑1 (MQ=60)
                                                                                            AACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGATGAG                                                        >  SRR19973063.5010089/1‑150 (MQ=60)
                                                                                              CAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTA                                                      <  SRR19973063.4140148/150‑1 (MQ=60)
                                                                                              CAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTA                                                      <  SRR19973063.1956182/150‑1 (MQ=60)
                                                                                               AATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTAT                                                     >  SRR19973063.2043471/1‑150 (MQ=60)
                                                                                                ATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATC                                                    >  SRR19973063.1936166/1‑150 (MQ=60)
                                                                                                    ATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCA                                                >  SRR19973063.653418/1‑150 (MQ=60)
                                                                                                     TAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAA                                               >  SRR19973063.1264789/1‑150 (MQ=60)
                                                                                                        TGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAA                                            >  SRR19973063.3793425/1‑150 (MQ=60)
                                                                                                        TGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACAGTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCCGCAAGAA                                            >  SRR19973063.3031391/1‑150 (MQ=60)
                                                                                                                CGGGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCC                                    >  SRR19973063.4809505/1‑150 (MQ=60)
                                                                                                                  GGGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGC                                  <  SRR19973063.817501/150‑1 (MQ=60)
                                                                                                                   GGTTATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCT                                 <  SRR19973063.3777145/150‑1 (MQ=60)
                                                                                                                       ATCATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTAC                             >  SRR19973063.3783589/1‑150 (MQ=60)
                                                                                                                         CATCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCA                           >  SRR19973063.1878519/1‑150 (MQ=60)
                                                                                                                           TCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACA                         <  SRR19973063.3226956/150‑1 (MQ=60)
                                                                                                                           TCTCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACA                         <  SRR19973063.4699641/150‑1 (MQ=60)
                                                                                                                             TCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAAC                       <  SRR19973063.356765/150‑1 (MQ=60)
                                                                                                                             TCCCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAAC                       >  SRR19973063.854875/1‑150 (MQ=60)
                                                                                                                               CCAGTATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGT                     >  SRR19973063.75741/1‑150 (MQ=60)
                                                                                                                                   TATATCCATACTACCGATAAGGTTATTTACTCACCCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTANCAGCAAGAATACTCGCCGCTTTACCACAACGTGGAT                 <  SRR19973063.2687652/150‑1 (MQ=60)
                                                                                                                                    ATATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATG                <  SRR19973063.910575/150‑1 (MQ=60)
                                                                                                                                      ATCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAG              <  SRR19973063.2366180/150‑1 (MQ=60)
                                                                                                                                       TCCATACTAACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGG             >  SRR19973063.4873964/1‑150 (MQ=60)
                                                                                                                                                ACGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAA    <  SRR19973063.4972715/150‑1 (MQ=60)
                                                                                                                                                ACGATAAGGTTATTTACTCAACCAGGCATAAACAGTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAA    >  SRR19973063.2503596/1‑150 (MQ=60)
                                                                                                                                                 CGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATTAGAGGGATGAAAA   >  SRR19973063.2282106/1‑150 (MQ=60)
                                                                                                                                                 CGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATTAGAGGGATGAAAA   >  SRR19973063.2282101/1‑150 (MQ=60)
                                                                                                                                                 CGATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAA   >  SRR19973063.328200/1‑150 (MQ=60)
                                                                                                                                                  GATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  <  SRR19973063.1773361/150‑1 (MQ=60)
                                                                                                                                                  GATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  <  SRR19973063.3781659/150‑1 (MQ=60)
                                                                                                                                                  GATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  <  SRR19973063.3781773/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAA  >  CP009273/1219387‑1219682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑