Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,337,533 G→T 100% A18E (GCA→GAA)  osmB ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,337,5330GT100.0% 611.0 / NA 183A18E (GCA→GAA) osmBlipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (96/87);  total (96/87)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 183 total aligned reads displayed.

AATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAAT  >  CP009273/1337388‑1337676
                                                                                                                                                 |                                                                                                                                               
aaTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTcc                                                                                                                                               >  2:317550/1‑148 (MQ=255)
 aTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCa                                                                                                                                              <  2:363428/148‑1 (MQ=255)
  tAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCaa                                                                                                                                             >  2:1546482/1‑148 (MQ=255)
   aaCACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCaaa                                                                                                                                            >  2:2330528/1‑148 (MQ=255)
     cacCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGt                                                                                                                                          <  2:2479225/148‑1 (MQ=255)
     cacCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGt                                                                                                                                          <  2:2663724/148‑1 (MQ=255)
      acCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAAGAGGCACTCAGAGACATTTCCAAAGTa                                                                                                                                         >  1:1024517/1‑148 (MQ=255)
      acCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTa                                                                                                                                         <  1:1380025/148‑1 (MQ=255)
      acCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTa                                                                                                                                         <  1:78364/148‑1 (MQ=255)
       ccTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTaa                                                                                                                                        <  1:228537/148‑1 (MQ=255)
        cTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAt                                                                                                                                       >  1:3311066/1‑148 (MQ=255)
         tCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAtt                                                                                                                                      >  2:3033064/1‑148 (MQ=255)
         tCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAtt                                                                                                                                      >  1:972702/1‑148 (MQ=255)
         tCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAtt                                                                                                                                      >  1:2814647/1‑148 (MQ=255)
          ccGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTg                                                                                                                                     <  1:2886796/148‑1 (MQ=255)
                gCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGaa                                                                                                                               >  2:856514/1‑148 (MQ=255)
                gCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGaa                                                                                                                               >  1:2278166/1‑148 (MQ=255)
                  tGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACa                                                                                                                             >  2:134886/1‑148 (MQ=255)
                      ccaccTAATGGACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCcg                                                                                                                         <  2:55835/148‑1 (MQ=255)
                        accTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCcgcg                                                                                                                       <  2:1615730/148‑1 (MQ=255)
                         ccTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCgg                                                                                                                      >  1:2756259/1‑148 (MQ=255)
                           tAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTc                                                                                                                    <  2:224630/148‑1 (MQ=255)
                            aaTGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGACCAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCa                                                                                                                   <  1:2383773/148‑1 (MQ=255)
                             aTGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAt                                                                                                                  <  1:2918953/148‑1 (MQ=255)
                             aTGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAt                                                                                                                  >  2:640852/1‑148 (MQ=255)
                             aTGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAt                                                                                                                  >  2:3296302/1‑148 (MQ=255)
                               gTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAttt                                                                                                                <  2:652865/148‑1 (MQ=255)
                                 aCCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAttttt                                                                                                              <  1:1970454/148‑1 (MQ=255)
                                 aCCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAttttt                                                                                                              >  1:2275319/1‑148 (MQ=255)
                                 aCCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAttttt                                                                                                              >  1:62990/1‑148 (MQ=255)
                                 aCCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAttttt                                                                                                              <  1:504398/148‑1 (MQ=255)
                                   ccAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCCCTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAttttttt                                                                                                            >  1:574950/1‑148 (MQ=255)
                                   ccAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAttttttt                                                                                                            >  1:1293246/1‑148 (MQ=255)
                                    cAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTg                                                                                                           >  1:2161808/1‑148 (MQ=255)
                                         tACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACCGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGt                                                                                                      >  2:1011642/1‑148 (MQ=255)
                                         tACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAAAAGCCGCGGTCATTTTTTTGCTCGt                                                                                                      >  2:962245/1‑148 (MQ=255)
                                          aCTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTAGtt                                                                                                     >  1:2223287/1‑148 (MQ=255)
                                           cTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTa                                                                                                    >  1:703596/1‑148 (MQ=255)
                                           cTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTa                                                                                                    <  1:862746/148‑1 (MQ=255)
                                           cTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTa                                                                                                    <  2:180251/148‑1 (MQ=255)
                                            tGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTAc                                                                                                   >  1:2508804/1‑148 (MQ=255)
                                            tGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTAc                                                                                                   >  1:2455185/1‑148 (MQ=255)
                                              ccATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACaa                                                                                                 <  2:466906/148‑1 (MQ=255)
                                              ccATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACaa                                                                                                 <  1:2654908/148‑1 (MQ=255)
                                                 tCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACa                                                                                              >  2:2970141/1‑148 (MQ=255)
                                                   ggTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACata                                                                                            >  2:2781907/1‑148 (MQ=255)
                                                    gTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACataa                                                                                           <  2:254312/148‑1 (MQ=255)
                                                     tCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACataat                                                                                          <  2:2938662/148‑1 (MQ=255)
                                                       aGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATAc                                                                                        >  2:2049394/1‑148 (MQ=255)
                                                           cTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActctc                                                                                    <  2:2124632/148‑1 (MQ=255)
                                                           cTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActctc                                                                                    <  2:2124621/148‑1 (MQ=255)
                                                            tGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActctct                                                                                   >  2:3097523/1‑148 (MQ=255)
                                                            tGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActctct                                                                                   >  1:1735797/1‑148 (MQ=255)
                                                             gCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACtctctc                                                                                  <  1:3169496/148‑1 (MQ=255)
                                                              cACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGAGCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTcc                                                                                 >  2:1417259/1‑148 (MQ=255)
                                                              cACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTcc                                                                                 >  1:40915/1‑148 (MQ=255)
                                                                  gCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGaa                                                                             >  2:3145208/1‑148 (MQ=255)
                                                                    cTAATGCCCCTGCACCCGCGCCGATTGCGGTTTTGCGGTCGAGTTTAGACCAGTTAGATCAGTCACTGAGAGACATTTCCAAAGTTATTTCGAGAACAGCATCGGTCATTTTTTTGCTCGTTACATACTTAATTCTCTCTCATGAAtt                                                                           <  2:2459310/148‑1 (MQ=255)
                                                                    cTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAAtt                                                                           <  2:801863/148‑1 (MQ=255)
                                                                     tAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAAttt                                                                          <  1:1417679/148‑1 (MQ=255)
                                                                       aTGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAAGTTAt                                                                        >  2:2040741/1‑148 (MQ=255)
                                                                         gCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGa                                                                      <  2:1312775/148‑1 (MQ=255)
                                                                         gCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGa                                                                      >  1:706355/1‑148 (MQ=255)
                                                                                  cccGCGCCGATTGCGGTGTTGCGGTCCCGTGTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGaa                                                             <  2:784059/148‑1 (MQ=255)
                                                                                    cgcgCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGt                                                           <  1:2114530/148‑1 (MQ=255)
                                                                                        ccGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGc                                                       <  2:988683/148‑1 (MQ=255)
                                                                                        ccGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGc                                                       <  2:2161805/148‑1 (MQ=255)
                                                                                         cgcttGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGct                                                      <  2:2465498/145‑1 (MQ=255)
                                                                                          gATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctc                                                     <  1:1791399/148‑1 (MQ=255)
                                                                                           aTTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctct                                                    <  2:1913128/148‑1 (MQ=255)
                                                                                           aTTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctct                                                    >  2:1575327/1‑148 (MQ=255)
                                                                                            ttGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctc                                                   >  2:1367927/1‑148 (MQ=255)
                                                                                             tGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctct                                                  >  1:605887/1‑148 (MQ=255)
                                                                                             tGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctct                                                  >  1:607051/1‑148 (MQ=255)
                                                                                                ggTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctata                                               <  2:1472575/148‑1 (MQ=255)
                                                                                                       cGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctataactataa                                        <  2:1855441/148‑1 (MQ=255)
                                                                                                           cccGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGc                                    <  1:2456438/148‑1 (MQ=255)
                                                                                                               tttAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAg                                >  2:130144/1‑148 (MQ=255)
                                                                                                                ttAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGa                               >  1:1111355/1‑148 (MQ=255)
                                                                                                                   gACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATa                            <  1:3227568/148‑1 (MQ=255)
                                                                                                                   gACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATa                            <  1:3227566/148‑1 (MQ=255)
                                                                                                                    aCCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTTCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATaa                           >  1:2493356/1‑148 (MQ=255)
                                                                                                                     ccAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAg                          <  2:763256/148‑1 (MQ=255)
                                                                                                                     ccAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAg                          >  1:2808633/1‑148 (MQ=255)
                                                                                                                     ccAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAg                          >  2:497943/1‑148 (MQ=255)
                                                                                                                      cAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGt                         >  2:3181933/1‑148 (MQ=255)
                                                                                                                        gTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCt                       >  2:1611115/1‑148 (MQ=255)
                                                                                                                         ttAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTg                      <  2:3311066/148‑1 (MQ=255)
                                                                                                                             aaCAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGa                  >  1:2727076/1‑148 (MQ=255)
                                                                                                                                aGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAat               <  2:23007/148‑1 (MQ=255)
                                                                                                                                  gCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAatta             <  1:2679436/148‑1 (MQ=255)
                                                                                                                                  gCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAatta             <  2:1489313/148‑1 (MQ=255)
                                                                                                                                   cACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAattat            >  2:1144806/1‑148 (MQ=255)
                                                                                                                                    aCTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAattatt           <  1:3033064/148‑1 (MQ=255)
                                                                                                                                     cTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTc          >  2:2797486/1‑148 (MQ=255)
                                                                                                                                          agaCATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGa     >  1:910604/1‑148 (MQ=255)
                                                                                                                                            aCATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGaaa   <  2:9056/148‑1 (MQ=255)
                                                                                                                                             cATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAAt  <  2:62990/148‑1 (MQ=255)
                                                                                                                                             cATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAAt  <  2:1111355/148‑1 (MQ=255)
                                                                                                                                             cATTTCCAAAGTAAGTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAAt  <  2:2046018/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAAT  >  CP009273/1337388‑1337676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTC  >  CP009273/1337392‑1337682
                                                                                                                                             |                                                                                                                                                     
ACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAAGAGGCACTCAGAGACATTTCCAAAGTA                                                                                                                                               >  SRR19973070.1031781/1‑150 (MQ=60)
  ACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAT                                                                                                                                             >  SRR19973070.3332246/1‑150 (MQ=60)
  ACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAT                                                                                                                                             <  SRR19973070.79189/150‑1 (MQ=60)
  ACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAT                                                                                                                                             <  SRR19973070.1389380/150‑1 (MQ=60)
  ACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAT                                                                                                                                             <  SRR19973070.79326/150‑1 (MQ=60)
   CCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATT                                                                                                                                            >  SRR19973070.979644/1‑150 (MQ=60)
   CCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATT                                                                                                                                            >  SRR19973070.2832846/1‑150 (MQ=60)
   CCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATT                                                                                                                                            <  SRR19973070.230162/150‑1 (MQ=60)
      CCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCC                                                                                                                                         <  SRR19973070.2905486/150‑1 (MQ=60)
      CCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCC                                                                                                                                         <  SRR19973070.2906409/150‑1 (MQ=60)
          CAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAA                                                                                                                                     >  SRR19973070.2293301/1‑150 (MQ=60)
          CAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAA                                                                                                                                     >  SRR19973070.2186104/1‑150 (MQ=60)
              TGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGC                                                                                                                                 <  SRR19973070.2070035/150‑1 (MQ=60)
                   CACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGG                                                                                                                            >  SRR19973070.2773948/1‑150 (MQ=60)
                      CTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCA                                                                                                                         >  SRR19973070.2715089/1‑150 (MQ=60)
                        AATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGACCAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATT                                                                                                                       <  SRR19973070.2399519/150‑1 (MQ=60)
                        AATGTACCCAACGGACTGCCATCGGTCAGTACTGCACCGCCTAAGGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATT                                                                                                                       <  SRR19973070.2399665/150‑1 (MQ=60)
                         ATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTT                                                                                                                      <  SRR19973070.2937804/150‑1 (MQ=60)
                         ATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTT                                                                                                                      >  SRR19973070.1822053/1‑150 (MQ=60)
                           GTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTT                                                                                                                    >  SRR19973070.2290434/1‑150 (MQ=60)
                           GTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTT                                                                                                                    >  SRR19973070.63694/1‑150 (MQ=60)
                            TACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTT                                                                                                                   <  SRR19973070.3053566/150‑1 (MQ=60)
                             ACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCCCTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTT                                                                                                                  >  SRR19973070.578883/1‑150 (MQ=60)
                             ACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTT                                                                                                                  >  SRR19973070.579692/1‑150 (MQ=60)
                             ACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTT                                                                                                                  >  SRR19973070.2960177/1‑150 (MQ=60)
                             ACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTT                                                                                                                  >  SRR19973070.578880/1‑150 (MQ=60)
                             ACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTT                                                                                                                  >  SRR19973070.1302254/1‑150 (MQ=60)
                             ACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTT                                                                                                                  <  SRR19973070.507891/150‑1 (MQ=60)
                             ACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTT                                                                                                                  <  SRR19973070.1983769/150‑1 (MQ=60)
                              CCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTG                                                                                                                 >  SRR19973070.2176173/1‑150 (MQ=60)
                              CCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTG                                                                                                                 >  SRR19973070.2176170/1‑150 (MQ=60)
                                CAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCT                                                                                                               >  SRR19973070.3265687/1‑150 (MQ=60)
                                    GTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTT                                                                                                           >  SRR19973070.136237/1‑150 (MQ=60)
                                    GTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTAGTT                                                                                                           >  SRR19973070.2237882/1‑150 (MQ=60)
                                     TACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTA                                                                                                          >  SRR19973070.708610/1‑150 (MQ=60)
                                     TACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTA                                                                                                          >  SRR19973070.708768/1‑150 (MQ=60)
                                      ACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTAC                                                                                                         >  SRR19973070.2471234/1‑150 (MQ=60)
                                      ACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTAC                                                                                                         >  SRR19973070.2525124/1‑150 (MQ=60)
                                      ACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTAC                                                                                                         >  SRR19973070.2471273/1‑150 (MQ=60)
                                       CTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACA                                                                                                        <  SRR19973070.868749/150‑1 (MQ=60)
                                          CCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAAC                                                                                                     <  SRR19973070.2672179/150‑1 (MQ=60)
                                          CCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAAC                                                                                                     <  SRR19973070.2298857/150‑1 (MQ=60)
                                                   AGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTC                                                                                            >  SRR19973070.2891840/1‑150 (MQ=60)
                                                      ACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCT                                                                                         >  SRR19973070.1747827/1‑150 (MQ=60)
                                                        TGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCC                                                                                       <  SRR19973070.436291/150‑1 (MQ=60)
                                                        TGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCC                                                                                       >  SRR19973070.41439/1‑150 (MQ=60)
                                                        TGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCC                                                                                       >  SRR19973070.664312/1‑150 (MQ=60)
                                                         GCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCT                                                                                      <  SRR19973070.2605191/150‑1 (MQ=60)
                                                         GCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCT                                                                                      <  SRR19973070.3190006/150‑1 (MQ=60)
                                                              GCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATT                                                                                 <  SRR19973070.2499807/150‑1 (MQ=60)
                                                                 TAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTAT                                                                              <  SRR19973070.1426972/150‑1 (MQ=60)
                                                                 TAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTAT                                                                              <  SRR19973070.1427393/150‑1 (MQ=60)
                                                                   ATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGA                                                                            >  SRR19973070.711387/1‑150 (MQ=60)
                                                                                CGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAA                                                               <  SRR19973070.924030/150‑1 (MQ=60)
                                                                                CGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAA                                                               <  SRR19973070.2128704/150‑1 (MQ=60)
                                                                                 GCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGTCACTCAGAGACATTTCCAAAGTAATTGACAGAACAGCCGCGGTCATTTTTTTGCTCTTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAG                                                              <  SRR19973070.2260835/150‑1 (MQ=60)
                                                                                      GATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTC                                                         <  SRR19973070.1803774/150‑1 (MQ=60)
                                                                                       ATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCT                                                        >  SRR19973070.611299/1‑150 (MQ=60)
                                                                                       ATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCT                                                        >  SRR19973070.610118/1‑150 (MQ=60)
                                                                                          GCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATA                                                     <  SRR19973070.2681385/150‑1 (MQ=60)
                                                                                             GTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACT                                                  <  SRR19973070.2346005/150‑1 (MQ=60)
                                                                                                   CGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATA                                            <  SRR19973070.2814084/150‑1 (MQ=60)
                                                                                                       CCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTA                                        <  SRR19973070.2472492/150‑1 (MQ=60)
                                                                                                          GTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGA                                     >  SRR19973070.1118959/1‑150 (MQ=60)
                                                                                                          GTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGA                                     >  SRR19973070.1275244/1‑150 (MQ=60)
                                                                                                            TTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAAT                                   <  SRR19973070.621993/150‑1 (MQ=60)
                                                                                                              AGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTTCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAA                                 >  SRR19973070.2509531/1‑150 (MQ=60)
                                                                                                               GACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAG                                <  SRR19973070.3248417/150‑1 (MQ=60)
                                                                                                               GACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAG                                <  SRR19973070.3248415/150‑1 (MQ=60)
                                                                                                               GACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAG                                >  SRR19973070.2826790/1‑150 (MQ=60)
                                                                                                                ACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGT                               >  SRR19973070.2753736/1‑150 (MQ=60)
                                                                                                                ACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGT                               >  SRR19973070.1642590/1‑150 (MQ=60)
                                                                                                                       AGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGA                        >  SRR19973070.2744666/1‑150 (MQ=60)
                                                                                                                             GGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTAT                  <  SRR19973070.773858/150‑1 (MQ=60)
                                                                                                                              GCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATT                 <  SRR19973070.2696825/150‑1 (MQ=60)
                                                                                                                                ACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCC               <  SRR19973070.3052360/150‑1 (MQ=60)
                                                                                                                                    AGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGA           >  SRR19973070.917072/1‑150 (MQ=60)
                                                                                                                                        ACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATC       <  SRR19973070.319854/150‑1 (MQ=60)
                                                                                                                                        ACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATC       <  SRR19973070.335210/150‑1 (MQ=60)
                                                                                                                                         CATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCT      >  SRR19973070.443820/1‑150 (MQ=60)
                                                                                                                                         CATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCT      >  SRR19973070.3109586/1‑150 (MQ=60)
                                                                                                                                             TCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTC  >  SRR19973070.390243/1‑150 (MQ=60)
                                                                                                                                             |                                                                                                                                                     
ACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTC  >  CP009273/1337392‑1337682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑