Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,337,533 G→T 100% A18E (GCA→GAA)  osmB ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,337,5330GT99.5% 727.2 / ‑4.3 216A18E (GCA→GAA) osmBlipoprotein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (83/132);  total (84/132)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 216 total aligned reads displayed.

CCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTG  >  CP009273/1337386‑1337679
                                                                                                                                                   |                                                                                                                                                  
   aTAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCaaa                                                                                                                                               <  2:4624049/150‑1 (MQ=255)
    gaaCACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAg                                                                                                                                              <  2:3128799/149‑1 (MQ=255)
     aaCACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGt                                                                                                                                             >  2:737753/1‑150 (MQ=255)
      acacCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTa                                                                                                                                            <  2:1807548/150‑1 (MQ=255)
        acCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAt                                                                                                                                          >  2:4119660/1‑150 (MQ=255)
         ccTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAtt                                                                                                                                         >  2:5157034/1‑150 (MQ=255)
          cTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTg                                                                                                                                        <  2:2523605/150‑1 (MQ=255)
             cGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCa                                                                                                                                     <  2:2842806/150‑1 (MQ=255)
                cAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGaa                                                                                                                                  >  1:1281064/1‑150 (MQ=255)
                  gCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACa                                                                                                                                <  2:10662/150‑1 (MQ=255)
                    tGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGACCAGc                                                                                                                              >  2:5048833/1‑150 (MQ=255)
                    tGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGc                                                                                                                              >  2:1806477/1‑150 (MQ=255)
                      caccacCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCcg                                                                                                                            >  1:4251841/1‑150 (MQ=255)
                          accTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGt                                                                                                                        >  1:4579669/1‑150 (MQ=255)
                           ccTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTc                                                                                                                       <  2:4037964/150‑1 (MQ=255)
                              aaTGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAtt                                                                                                                    <  1:1251257/150‑1 (MQ=255)
                               aTGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAttt                                                                                                                   <  1:2162838/150‑1 (MQ=255)
                                   aCCCAACGTACTGCCATCTGTCAGTACTGCACCGCCTTATGCCCCTGCTCCCGCGACGATTGCGGTGTTGAGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTACAAAGTAATTGCCAGAACTGCCGCGGTCAttttttt                                                                                                               <  1:665203/150‑1 (MQ=255)
                                   aCCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAttttttt                                                                                                               >  2:3250329/1‑150 (MQ=255)
                                    cccAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTg                                                                                                              >  1:2834100/1‑150 (MQ=255)
                                       aaCGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTc                                                                                                           <  2:279017/150‑1 (MQ=255)
                                       aaCGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTc                                                                                                           >  1:3306817/1‑150 (MQ=255)
                                       aaCGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTc                                                                                                           <  2:700357/150‑1 (MQ=255)
                                        aCGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCg                                                                                                          <  2:2853711/150‑1 (MQ=255)
                                        aCGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCg                                                                                                          <  1:3743140/150‑1 (MQ=255)
                                          gTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGtt                                                                                                        <  2:1366582/150‑1 (MQ=255)
                                          gTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGtt                                                                                                        <  1:763507/150‑1 (MQ=255)
                                          gTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCGGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGtt                                                                                                        <  1:316536/150‑1 (MQ=255)
                                           tACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTa                                                                                                       >  1:4545970/1‑150 (MQ=255)
                                           tACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTa                                                                                                       >  1:1529055/1‑150 (MQ=255)
                                                 cATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACa                                                                                                 <  1:5120478/150‑1 (MQ=255)
                                                 cATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACa                                                                                                 <  1:4015602/150‑1 (MQ=255)
                                                 cATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACa                                                                                                 >  1:1710531/1‑150 (MQ=255)
                                                  aTCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACat                                                                                                <  2:1926564/150‑1 (MQ=255)
                                                     ggTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACataat                                                                                             >  2:2746883/1‑150 (MQ=255)
                                                     ggTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACataat                                                                                             >  1:928530/1‑150 (MQ=255)
                                                     ggTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACataat                                                                                             <  2:4595810/150‑1 (MQ=255)
                                                      gTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGTCCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACataata                                                                                            <  1:1861731/150‑1 (MQ=255)
                                                      gTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACataata                                                                                            <  1:4432231/150‑1 (MQ=255)
                                                       tCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATAc                                                                                           <  2:3643923/150‑1 (MQ=255)
                                                           tACTGCACCGCCTAAGGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActctc                                                                                       <  2:3370310/150‑1 (MQ=255)
                                                              tGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTcc                                                                                    >  2:1624932/1‑150 (MQ=255)
                                                                  ccGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGaa                                                                                >  1:816539/1‑150 (MQ=255)
                                                                     ccTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAAttt                                                                             <  1:4890655/150‑1 (MQ=255)
                                                                     ccTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAAttt                                                                             <  1:2731842/150‑1 (MQ=255)
                                                                       tAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTAt                                                                           >  1:1494086/1‑150 (MQ=255)
                                                                        aaTGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATg                                                                          <  1:2774541/150‑1 (MQ=255)
                                                                         aTGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGa                                                                         <  2:1770669/150‑1 (MQ=255)
                                                                         aTGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGa                                                                         <  2:2441849/150‑1 (MQ=255)
                                                                         aTGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGa                                                                         >  1:3254114/1‑150 (MQ=255)
                                                                            ccccTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTc                                                                      >  2:4207533/1‑150 (MQ=255)
                                                                             cccTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAACTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCa                                                                     <  1:4799278/150‑1 (MQ=255)
                                                                               cTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACg                                                                   >  2:797756/1‑150 (MQ=255)
                                                                                tGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACgg                                                                  <  1:4857078/150‑1 (MQ=255)
                                                                                  cACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGAACAGTTAGAACAGGCACTCAGAGACATTTACAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTAACGGaa                                                                <  2:2231653/150‑1 (MQ=255)
                                                                                   aCCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAg                                                               >  1:1043699/1‑150 (MQ=255)
                                                                                        cgcCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGc                                                          <  2:714107/150‑1 (MQ=255)
                                                                                          ccGAGTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctc                                                        <  2:1870677/150‑1 (MQ=255)
                                                                                           cGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctct                                                       <  1:2212641/150‑1 (MQ=255)
                                                                                           cGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctct                                                       <  2:1491193/150‑1 (MQ=255)
                                                                                            gATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctc                                                      >  1:1184699/1‑150 (MQ=255)
                                                                                            gATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctc                                                      >  1:410667/1‑150 (MQ=255)
                                                                                             aTTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctct                                                     <  1:576538/150‑1 (MQ=255)
                                                                                             aTTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctct                                                     <  1:3160194/150‑1 (MQ=255)
                                                                                              ttGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTcta                                                    <  1:4014364/150‑1 (MQ=255)
                                                                                                gCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctata                                                  >  2:640029/1‑150 (MQ=255)
                                                                                                gCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctata                                                  <  1:3370925/150‑1 (MQ=255)
                                                                                                   gtgtTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctataact                                               <  1:3676810/150‑1 (MQ=255)
                                                                                                        gCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTataat                                          <  2:2091763/150‑1 (MQ=255)
                                                                                                         cGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTataata                                         <  1:4909621/150‑1 (MQ=255)
                                                                                                          ggTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAg                                        >  1:4740895/1‑150 (MQ=255)
                                                                                                            tCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCt                                      >  1:1233733/1‑150 (MQ=255)
                                                                                                             cccGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTa                                     >  1:2394619/1‑150 (MQ=255)
                                                                                                             cccGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGAGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTa                                     <  1:1415950/150‑1 (MQ=255)
                                                                                                               cGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAg                                   >  2:3192694/1‑150 (MQ=255)
                                                                                                                  ttAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAAt                                <  2:4989094/150‑1 (MQ=255)
                                                                                                                   tAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACCGCCGCGGTCATTTTTTTGCTCGTTACACACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATACCTATAAGAGCTAAGAATa                               >  2:2959108/1‑150 (MQ=255)
                                                                                                                           ttAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGt                       <  1:3714222/150‑1 (MQ=255)
                                                                                                                           ataccaCAGGGACTCAGACACAATTCCAAAGTAATTGCCAGACCAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCTCGGAAGTAAGCTCTCTATTACTATAATAGCTAAGAATAAGTCTGGt                       <  1:3028991/145‑1 (MQ=255)
                                                                                                                            tAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTg                      <  1:3110806/150‑1 (MQ=255)
                                                                                                                            tAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTg                      <  1:1744870/150‑1 (MQ=255)
                                                                                                                             aGAACAGGCACTCATCGACATTTCCAATGTAATTGCAAGAACAGCCGCGGTCTTTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGa                     <  2:4448131/150‑1 (MQ=255)
                                                                                                                             aGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGa                     >  2:4808389/1‑150 (MQ=255)
                                                                                                                                 cAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAatt                 <  1:3550512/150‑1 (MQ=255)
                                                                                                                                 cAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAatt                 <  1:5157034/150‑1 (MQ=255)
                                                                                                                                 cAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAatt                 <  2:200546/150‑1 (MQ=255)
                                                                                                                                  gggCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAatta                >  1:2319389/2‑150 (MQ=255)
                                                                                                                                  aGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAatta                >  2:3212324/1‑150 (MQ=255)
                                                                                                                                   ggCACTCTGAGTCATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTTCAAACATAATTCTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAattat               <  1:1450961/150‑1 (MQ=255)
                                                                                                                                   ggCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAattat               <  2:3988618/150‑1 (MQ=255)
                                                                                                                                    gCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAattatt              >  2:1345087/1‑150 (MQ=255)
                                                                                                                                      aCTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTTAGAATAAGTCTGGTGAAATTATTcc            <  2:861090/150‑1 (MQ=255)
                                                                                                                                      aCTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTcc            <  1:2289159/150‑1 (MQ=255)
                                                                                                                                        tCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGt          >  2:4018128/1‑150 (MQ=255)
                                                                                                                                        tCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGt          >  2:1740187/1‑150 (MQ=255)
                                                                                                                                         cAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTg         >  2:4298737/1‑150 (MQ=255)
                                                                                                                                         cAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTg         <  2:1519869/150‑1 (MQ=255)
                                                                                                                                         cAGAGACATTTCCAAAGTAATTGCCAGAACAGCAGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTg         <  2:1011611/150‑1 (MQ=255)
                                                                                                                                            tgaCATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGaaa      >  1:129841/2‑150 (MQ=255)
                                                                                                                                               cATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCt   <  2:4085650/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTG  >  CP009273/1337386‑1337679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTG  >  CP009273/1337384‑1337679
                                                                                                                                                     |                                                                                                                                                  
GACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTT                                                                                                                                                    <  SRR19973063.4351352/150‑1 (MQ=60)
 ACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTC                                                                                                                                                   >  SRR19973063.1706732/1‑150 (MQ=60)
  CCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCC                                                                                                                                                  >  SRR19973063.1512002/1‑150 (MQ=60)
             TCCGACAGCTGCACCACCTAATGTACCCAACGTACTACCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGC                                                                                                                                       <  SRR19973063.2116482/150‑1 (MQ=60)
                GACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAG                                                                                                                                    >  SRR19973063.322180/1‑150 (MQ=60)
                  CAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAA                                                                                                                                  >  SRR19973063.1284884/1‑150 (MQ=60)
                      TGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGC                                                                                                                              >  SRR19973063.801908/1‑150 (MQ=60)
                       GCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCC                                                                                                                             <  SRR19973063.4904854/150‑1 (MQ=60)
                        CACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCG                                                                                                                            >  SRR19973063.4265384/1‑150 (MQ=60)
                            ACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGT                                                                                                                        >  SRR19973063.4594319/1‑150 (MQ=60)
                              CTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGCGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCA                                                                                                                      <  SRR19973063.3174510/150‑1 (MQ=60)
                                AATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATT                                                                                                                    <  SRR19973063.1254983/150‑1 (MQ=60)
                                 ATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTT                                                                                                                   <  SRR19973063.2169610/150‑1 (MQ=60)
                                     ACCCAACGTACTGCCATCTGTCAGTACTGCACCGCCTTATGCCCCTGCTCCCGCGACGATTGCGGTGTTGAGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTACAAAGTAATTGCCAGAACTGCCGCGGTCATTTTTTT                                                                                                               <  SRR19973063.667146/150‑1 (MQ=60)
                                      CCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTG                                                                                                              >  SRR19973063.2843201/1‑150 (MQ=60)
                                      CCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTG                                                                                                              <  SRR19973063.2304884/150‑1 (MQ=60)
                                       CCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGC                                                                                                             <  SRR19973063.2484595/150‑1 (MQ=60)
                                         AACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTC                                                                                                           >  SRR19973063.1063704/1‑150 (MQ=60)
                                         AACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTC                                                                                                           >  SRR19973063.3317392/1‑150 (MQ=60)
                                         AACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTC                                                                                                           >  SRR19973063.3317515/1‑150 (MQ=60)
                                         AACGTACTGCCAGCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTC                                                                                                           >  SRR19973063.306032/1‑150 (MQ=60)
                                          ACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTACTTGCCAGAACAGCCGCGGTCATTTTTTTGCTCG                                                                                                          >  SRR19973063.3212641/1‑150 (MQ=60)
                                          ACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCG                                                                                                          <  SRR19973063.3755100/150‑1 (MQ=60)
                                          ACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCG                                                                                                          <  SRR19973063.4242989/150‑1 (MQ=60)
                                            GTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTT                                                                                                        <  SRR19973063.765739/150‑1 (MQ=60)
                                            GTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCGGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTT                                                                                                        <  SRR19973063.317464/150‑1 (MQ=60)
                                             TACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTA                                                                                                       >  SRR19973063.1533743/1‑150 (MQ=60)
                                             TACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTA                                                                                                       >  SRR19973063.4560538/1‑150 (MQ=60)
                                                TGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAA                                                                                                    <  SRR19973063.3995379/150‑1 (MQ=60)
                                                TGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAA                                                                                                    >  SRR19973063.2232348/1‑150 (MQ=60)
                                                   CATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACA                                                                                                 >  SRR19973063.2778518/1‑150 (MQ=60)
                                                   CATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACA                                                                                                 >  SRR19973063.1970655/1‑150 (MQ=60)
                                                   CATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACA                                                                                                 <  SRR19973063.1727779/150‑1 (MQ=60)
                                                   CATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACA                                                                                                 >  SRR19973063.1715742/1‑150 (MQ=60)
                                                   CATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACA                                                                                                 <  SRR19973063.4028486/150‑1 (MQ=60)
                                                   CATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACA                                                                                                 >  SRR19973063.4549443/1‑150 (MQ=60)
                                                   CATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACA                                                                                                 <  SRR19973063.5136952/150‑1 (MQ=60)
                                                    ATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACAT                                                                                                <  SRR19973063.3140827/150‑1 (MQ=60)
                                                    ATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGAAATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACAT                                                                                                <  SRR19973063.3909894/150‑1 (MQ=60)
                                                      CGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAA                                                                                              <  SRR19973063.5150648/150‑1 (MQ=60)
                                                       GGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAAT                                                                                             >  SRR19973063.931296/1‑150 (MQ=60)
                                                        GTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGTCCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATA                                                                                            <  SRR19973063.1867544/150‑1 (MQ=60)
                                                        GTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATA                                                                                            <  SRR19973063.4446453/150‑1 (MQ=60)
                                                         TCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCA‑TTTTTTGCTCGTTACAAACATAATACT                                                                                          <  SRR19973063.4574442/150‑1 (MQ=60)
                                                         TCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCA‑TTTTTTGCTCGTTACAAACATAATACT                                                                                          <  SRR19973063.4574133/150‑1 (MQ=60)
                                                          CAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACT                                                                                          <  SRR19973063.4596459/150‑1 (MQ=60)
                                                           AGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTC                                                                                         >  SRR19973063.1257364/1‑150 (MQ=60)
                                                            GTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGGTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCT                                                                                        >  SRR19973063.2183733/1‑150 (MQ=60)
                                                             TACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTC                                                                                       >  SRR19973063.3303202/1‑150 (MQ=60)
                                                                 GCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCT                                                                                   <  SRR19973063.828797/150‑1 (MQ=60)
                                                                    CCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAA                                                                                >  SRR19973063.818899/1‑150 (MQ=60)
                                                                       CCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTT                                                                             <  SRR19973063.4906433/150‑1 (MQ=60)
                                                                       CCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTT                                                                             <  SRR19973063.140555/150‑1 (MQ=60)
                                                                       CCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTT                                                                             <  SRR19973063.2740659/150‑1 (MQ=60)
                                                                       CCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTT                                                                             >  SRR19973063.2839496/1‑150 (MQ=60)
                                                                        CTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTA                                                                            <  SRR19973063.606835/150‑1 (MQ=60)
                                                                         TAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTAT                                                                           >  SRR19973063.1498665/1‑150 (MQ=60)
                                                                          AATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATG                                                                          <  SRR19973063.2783480/150‑1 (MQ=60)
                                                                           ATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGA                                                                         >  SRR19973063.3264553/1‑150 (MQ=60)
                                                                               CCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAACTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCA                                                                     <  SRR19973063.4814618/150‑1 (MQ=60)
                                                                                CCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCAC                                                                    <  SRR19973063.745442/150‑1 (MQ=60)
                                                                                  TGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGG                                                                  <  SRR19973063.4872431/150‑1 (MQ=60)
                                                                                  TGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGG                                                                  <  SRR19973063.4872725/150‑1 (MQ=60)
                                                                                     ACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAG                                                               >  SRR19973063.1046860/1‑150 (MQ=60)
                                                                                      CCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGT                                                              <  SRR19973063.148706/150‑1 (MQ=60)
                                                                                             CGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCT                                                       <  SRR19973063.2219618/150‑1 (MQ=60)
                                                                                              GATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTC                                                      >  SRR19973063.1188266/1‑150 (MQ=60)
                                                                                              GATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTC                                                      >  SRR19973063.411823/1‑150 (MQ=60)
                                                                                               ATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCT                                                     <  SRR19973063.578234/150‑1 (MQ=60)
                                                                                               ATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCT                                                     <  SRR19973063.575216/150‑1 (MQ=60)
                                                                                               ATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCT                                                     <  SRR19973063.3170366/150‑1 (MQ=60)
                                                                                                TTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTA                                                    <  SRR19973063.3733371/150‑1 (MQ=60)
                                                                                                TTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTA                                                    <  SRR19973063.4027245/150‑1 (MQ=60)
                                                                                                TTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTA                                                    <  SRR19973063.4027298/150‑1 (MQ=60)
                                                                                                 TGCGGTGTAGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTAT                                                   <  SRR19973063.1786834/150‑1 (MQ=60)
                                                                                                  GCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATA                                                  <  SRR19973063.3381658/150‑1 (MQ=60)
                                                                                                    GGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAAC                                                <  SRR19973063.4119446/150‑1 (MQ=60)
                                                                                                     GTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACT                                               <  SRR19973063.3688580/150‑1 (MQ=60)
                                                                                                        tcgcggccccgtctaccccagaTAGAACAGGCACTCAGAGACATTTCCAACGTAATTGCCAGCACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATA                                            <  SRR19973063.4347073/128‑1 (MQ=60)
                                                                                                        TTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATA                                            <  SRR19973063.2966513/150‑1 (MQ=60)
                                                                                                         TGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAA                                           >  SRR19973063.1285494/1‑150 (MQ=60)
                                                                                                           CGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATA                                         <  SRR19973063.4925473/150‑1 (MQ=60)
                                                                                                            GGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAG                                        >  SRR19973063.4917240/1‑150 (MQ=60)
                                                                                                            GGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAG                                        >  SRR19973063.917606/1‑150 (MQ=60)
                                                                                                            GGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAG                                        >  SRR19973063.4755992/1‑150 (MQ=60)
                                                                                                              TCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCT                                      >  SRR19973063.1237411/1‑150 (MQ=60)
                                                                                                               CCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTA                                     >  SRR19973063.2402281/1‑150 (MQ=60)
                                                                                                               CCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTA                                     <  SRR19973063.1248770/150‑1 (MQ=60)
                                                                                                               CCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAAGTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTA                                     >  SRR19973063.2402085/1‑150 (MQ=60)
                                                                                                               CCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGAGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTA                                     <  SRR19973063.1420254/150‑1 (MQ=60)
                                                                                                                    TTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAAT                                <  SRR19973063.1447280/150‑1 (MQ=60)
                                                                                                                          CAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCT                          >  SRR19973063.3554384/1‑150 (MQ=60)
                                                                                                                             TTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGTAAGTAATCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGT                       <  SRR19973063.3725956/150‑1 (MQ=60)
                                                                                                                             TTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGT                       <  SRR19973063.3726098/150‑1 (MQ=60)
                                                                                                                              TAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTG                      <  SRR19973063.3120816/150‑1 (MQ=60)
                                                                                                                              TAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTG                      <  SRR19973063.1750174/150‑1 (MQ=60)
                                                                                                                                 AACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAA                   <  SRR19973063.4938964/150‑1 (MQ=60)
                                                                                                                                 AACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAA                   <  SRR19973063.4938722/150‑1 (MQ=60)
                                                                                                                                   CAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATT                 <  SRR19973063.5173622/150‑1 (MQ=60)
                                                                                                                                   CAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATT                 <  SRR19973063.3561891/150‑1 (MQ=60)
                                                                                                                                    GGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTA                >  SRR19973063.2326803/1‑150 (MQ=60)
                                                                                                                                     GGCACTCTGAGTCATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTTCAAACATAATTCTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTAT               <  SRR19973063.1455388/150‑1 (MQ=60)
                                                                                                                                       CACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTC             >  SRR19973063.1201731/1‑150 (MQ=60)
                                                                                                                                       CACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTC             >  SRR19973063.2765033/1‑150 (MQ=60)
                                                                                                                                        ACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCC            <  SRR19973063.2296467/150‑1 (MQ=60)
                                                                                                                                         CTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTCTAATAGCTAAGAATAAGTCTGGTGAAATTATTCCG           >  SRR19973063.1566621/1‑150 (MQ=60)
                                                                                                                                              TGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAA      >  SRR19973063.130257/1‑150 (MQ=60)
                                                                                                                                              AGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAA      >  SRR19973063.3640308/1‑150 (MQ=60)
                                                                                                                                                  ATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTG  <  SRR19973063.4793731/150‑1 (MQ=60)
                                                                                                                                                  ATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTG  <  SRR19973063.1440182/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
GACCAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTG  >  CP009273/1337384‑1337679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑