Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,337,533 G→T 62.2% A18E (GCA→GAA)  osmB ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,337,5330GT62.2% 169.8 / 312.2 232A18E (GCA→GAA) osmBlipoprotein
Reads supporting (aligned to +/- strand):  ref base G (35/53);  new base T (64/80);  total (99/133)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

CAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTG  >  CP009273/1337387‑1337679
                                                                                                                                                  |                                                                                                                                                  
cAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCa                                                                                                                                                 >  2:3291840/1‑150 (MQ=255)
 actAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCaa                                                                                                                                                <  2:3560293/148‑1 (MQ=255)
   tAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAg                                                                                                                                              >  2:4242266/1‑150 (MQ=255)
       acCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAAt                                                                                                                                          <  2:2661982/150‑1 (MQ=255)
        ccTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAtt                                                                                                                                         >  1:871998/1‑150 (MQ=255)
         cTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTg                                                                                                                                        <  1:758464/150‑1 (MQ=255)
         cTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTg                                                                                                                                        <  1:372115/150‑1 (MQ=255)
             gACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAg                                                                                                                                    <  2:1415811/150‑1 (MQ=255)
             gACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAg                                                                                                                                    <  2:3876866/150‑1 (MQ=255)
               cAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGaa                                                                                                                                  <  2:2816321/150‑1 (MQ=255)
                 gCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACa                                                                                                                                <  2:775478/150‑1 (MQ=255)
                 gCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACa                                                                                                                                <  2:1799317/150‑1 (MQ=255)
                 gCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACa                                                                                                                                >  2:1198018/1‑150 (MQ=255)
                 gCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACa                                                                                                                                <  1:4211164/150‑1 (MQ=255)
                    gCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGcc                                                                                                                             <  2:3969551/150‑1 (MQ=255)
                      accaccTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCcgc                                                                                                                           >  1:444470/1‑150 (MQ=255)
                      accaccTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCcgc                                                                                                                           >  2:1342255/1‑150 (MQ=255)
                            tAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCAt                                                                                                                     >  2:4470776/1‑150 (MQ=255)
                            tAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCAt                                                                                                                     >  1:1230353/1‑150 (MQ=255)
                                gTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGACCAGCCGCGGTCAttttt                                                                                                                 >  1:2879899/1‑150 (MQ=255)
                                gTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCAttttt                                                                                                                 >  2:1908598/1‑150 (MQ=255)
                                gTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCAttttt                                                                                                                 <  1:162264/150‑1 (MQ=255)
                                 tACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCAtttttt                                                                                                                >  1:1998237/1‑150 (MQ=255)
                                     cAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCt                                                                                                            >  2:2907479/1‑150 (MQ=255)
                                      aaCGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTc                                                                                                           <  1:168453/150‑1 (MQ=255)
                                      aaCGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTc                                                                                                           >  2:3301189/1‑150 (MQ=255)
                                         gTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGtt                                                                                                        >  2:3740091/1‑150 (MQ=255)
                                         gTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGtt                                                                                                        <  2:554093/150‑1 (MQ=255)
                                           aCTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTAc                                                                                                      <  1:2425481/150‑1 (MQ=255)
                                           aCTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTAc                                                                                                      >  2:4620710/1‑150 (MQ=255)
                                            cTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACa                                                                                                     >  1:342492/1‑150 (MQ=255)
                                            cTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACa                                                                                                     <  1:4096214/150‑1 (MQ=255)
                                             tGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACaa                                                                                                    >  2:722378/1‑150 (MQ=255)
                                             tGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACaa                                                                                                    >  2:3151693/1‑150 (MQ=255)
                                                cATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACa                                                                                                 >  2:2230112/1‑150 (MQ=255)
                                                cATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACa                                                                                                 >  2:15693/1‑150 (MQ=255)
                                                 aTCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACat                                                                                                <  2:4381124/150‑1 (MQ=255)
                                                   cGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACataa                                                                                              <  1:696026/150‑1 (MQ=255)
                                                   cGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTGTGCTCGTTACAAACataa                                                                                              >  1:4623898/1‑150 (MQ=255)
                                                    ggTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACataat                                                                                             <  2:2622074/150‑1 (MQ=255)
                                                         gTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActct                                                                                        <  1:4269161/150‑1 (MQ=255)
                                                         gTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActct                                                                                        <  2:1061568/150‑1 (MQ=255)
                                                           aCTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActctct                                                                                      <  1:602/150‑1 (MQ=255)
                                                           aCTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActctct                                                                                      >  2:3089033/1‑150 (MQ=255)
                                                           aCTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATActctct                                                                                      >  2:3089273/1‑150 (MQ=255)
                                                             tGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTcc                                                                                    <  2:2503823/150‑1 (MQ=255)
                                                              gCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCt                                                                                   >  2:3931776/1‑150 (MQ=255)
                                                              gCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCt                                                                                   >  2:3930629/1‑150 (MQ=255)
                                                              gCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCt                                                                                   <  2:3783677/150‑1 (MQ=255)
                                                               cACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTg                                                                                  <  2:2380588/150‑1 (MQ=255)
                                                               cACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTg                                                                                  >  1:4298912/1‑150 (MQ=255)
                                                               cACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTg                                                                                  <  1:848377/150‑1 (MQ=255)
                                                                aCCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGa                                                                                 <  1:3036132/150‑1 (MQ=255)
                                                                aCCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGa                                                                                 <  1:4058871/150‑1 (MQ=255)
                                                                  cGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAAt                                                                               >  1:1492500/1‑150 (MQ=255)
                                                                     cTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAAttt                                                                             <  1:935045/149‑1 (MQ=255)
                                                                     cTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTa                                                                            >  2:406474/1‑150 (MQ=255)
                                                                      tAATGCCCCTGCACCCGCGCCGAGTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGTCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTAt                                                                           <  1:2271107/150‑1 (MQ=255)
                                                                       aaTGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATg                                                                          <  2:2064531/150‑1 (MQ=255)
                                                                         tGCCCCCGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGCACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGAt                                                                        <  1:117096/150‑1 (MQ=255)
                                                                             ccTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCAc                                                                    <  2:2333734/150‑1 (MQ=255)
                                                                                 cACCCGCGCCGATTGCGGTGTTGCTGTCCCGTTTAGACCAGTTAGACCAGGCACTCAGAGACATTTCCAAAGTAATGGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGaa                                                                <  1:362166/150‑1 (MQ=255)
                                                                                  aCCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAg                                                               >  1:2493831/1‑150 (MQ=255)
                                                                                   cccGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGt                                                              <  1:783797/150‑1 (MQ=255)
                                                                                    ccGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTa                                                             <  1:74185/150‑1 (MQ=255)
                                                                                        gcCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGct                                                         >  2:2312785/1‑150 (MQ=255)
                                                                                        gcCGAGTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGct                                                         <  2:2866021/150‑1 (MQ=255)
                                                                                          cGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctct                                                       >  1:4394178/1‑150 (MQ=255)
                                                                                           gATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctc                                                      <  1:1067855/150‑1 (MQ=255)
                                                                                           gATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctc                                                      <  2:2135005/150‑1 (MQ=255)
                                                                                           gATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctc                                                      <  1:1706407/150‑1 (MQ=255)
                                                                                            aTTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGctctca                                                     <  1:2012086/150‑2 (MQ=255)
                                                                                             ttGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTcta                                                    <  1:2799213/150‑1 (MQ=255)
                                                                                                 ggTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctataac                                                <  1:1003363/150‑1 (MQ=255)
                                                                                                 ggTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctataac                                                <  2:4047630/150‑1 (MQ=255)
                                                                                                      tGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTctataactataa                                           <  2:4348414/150‑1 (MQ=255)
                                                                                                       gCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTataat                                          >  1:1413026/1‑150 (MQ=255)
                                                                                                       gCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTataat                                          <  2:661622/150‑1 (MQ=255)
                                                                                                        cGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTataata                                         <  1:1762235/150‑1 (MQ=255)
                                                                                                        cGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTataata                                         <  1:1763316/150‑1 (MQ=255)
                                                                                                        cGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTataata                                         <  1:3356866/150‑1 (MQ=255)
                                                                                                        cGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTataata                                         <  1:2151416/150‑1 (MQ=255)
                                                                                                           tCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCt                                      <  2:4055757/150‑1 (MQ=255)
                                                                                                            cccGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTa                                     >  1:4739042/1‑150 (MQ=255)
                                                                                                                 ttAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAAt                                <  2:465331/150‑1 (MQ=255)
                                                                                                                   aGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCACTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATaa                              >  1:1931529/1‑150 (MQ=255)
                                                                                                                    gACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAg                             <  2:871998/150‑1 (MQ=255)
                                                                                                                    gACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAg                             >  2:2170185/1‑150 (MQ=255)
                                                                                                                     aCCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGt                            <  1:2335104/150‑1 (MQ=255)
                                                                                                                        aGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTg                         >  1:4482327/1‑150 (MQ=255)
                                                                                                                          ttAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGt                       <  1:2548456/150‑1 (MQ=255)
                                                                                                                           tAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTg                      <  2:2838953/150‑1 (MQ=255)
                                                                                                                            aGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGa                     >  1:990014/1‑150 (MQ=255)
                                                                                                                                cAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAatt                 <  2:577181/150‑1 (MQ=255)
                                                                                                                                 aGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAatta                <  1:3115441/150‑1 (MQ=255)
                                                                                                                                      cTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCg           <  1:2657468/150‑1 (MQ=255)
                                                                                                                                       tCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGt          <  2:1648748/150‑1 (MQ=255)
                                                                                                                                        cAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTg         >  2:1521598/1‑150 (MQ=255)
                                                                                                                                        cAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTg         <  1:1521853/150‑1 (MQ=255)
                                                                                                                                               aTTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTg  <  1:4630314/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CAATAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTG  >  CP009273/1337387‑1337679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTC  >  CP009273/1337390‑1337682
                                                                                                                                               |                                                                                                                                                     
TAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAG                                                                                                                                                 >  SRR19973081.4308949/1‑150 (MQ=60)
     CCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATT                                                                                                                                            >  SRR19973081.877750/1‑150 (MQ=60)
      CTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTG                                                                                                                                           <  SRR19973081.763304/150‑1 (MQ=60)
      CTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTG                                                                                                                                           <  SRR19973081.763376/150‑1 (MQ=60)
      CTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTG                                                                                                                                           <  SRR19973081.763392/150‑1 (MQ=60)
      CTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTG                                                                                                                                           <  SRR19973081.374593/150‑1 (MQ=60)
              GCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACA                                                                                                                                   <  SRR19973081.4236888/150‑1 (MQ=60)
               CTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAG                                                                                                                                  <  SRR19973081.3397394/150‑1 (MQ=60)
               CTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAG                                                                                                                                  <  SRR19973081.481665/150‑1 (MQ=60)
                TGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGC                                                                                                                                 >  SRR19973081.2436402/1‑150 (MQ=60)
                   ACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGC                                                                                                                              >  SRR19973081.447497/1‑150 (MQ=60)
                   ACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGC                                                                                                                              >  SRR19973081.447501/1‑150 (MQ=60)
                   ACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAAATTCCAGAACACCCGC                                                                                                                              >  SRR19973081.468574/1‑150 (MQ=60)
                         TAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCAT                                                                                                                        >  SRR19973081.1238412/1‑150 (MQ=60)
                          AATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATT                                                                                                                       >  SRR19973081.1107529/1‑150 (MQ=60)
                           ATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTT                                                                                                                      <  SRR19973081.970574/150‑1 (MQ=60)
                           ATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTT                                                                                                                      >  SRR19973081.2004458/1‑150 (MQ=60)
                             GTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGACCAGCCGCGGTCATTTTT                                                                                                                    >  SRR19973081.2897567/1‑150 (MQ=60)
                             GTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTT                                                                                                                    >  SRR19973081.2897151/1‑150 (MQ=60)
                             GTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCTAAGTAATTGCCAGAACAGCCGCGGTCATTTTG                                                                                                                    <  SRR19973081.2935661/150‑1 (MQ=60)
                             GTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTT                                                                                                                    <  SRR19973081.163753/150‑1 (MQ=60)
                              TACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTT                                                                                                                   >  SRR19973081.2010721/1‑150 (MQ=60)
                                CCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTG                                                                                                                 <  SRR19973081.1053177/150‑1 (MQ=60)
                                   AACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTC                                                                                                              <  SRR19973081.169985/150‑1 (MQ=60)
                                        ACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTAC                                                                                                         <  SRR19973081.4103634/150‑1 (MQ=60)
                                        ACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTAC                                                                                                         <  SRR19973081.2440356/150‑1 (MQ=60)
                                         CTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACA                                                                                                        >  SRR19973081.344893/1‑150 (MQ=60)
                                         CTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACA                                                                                                        <  SRR19973081.4121098/150‑1 (MQ=60)
                                         CTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACA                                                                                                        <  SRR19973081.4121430/150‑1 (MQ=60)
                                                CGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAA                                                                                                 <  SRR19973081.700685/150‑1 (MQ=60)
                                                CGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAA                                                                                                 >  SRR19973081.833382/1‑150 (MQ=60)
                                                CGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAA                                                                                                 <  SRR19973081.2208026/150‑1 (MQ=60)
                                                CGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTGTGCTCGTTACAAACATAA                                                                                                 >  SRR19973081.4651973/1‑150 (MQ=60)
                                                  GTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATA                                                                                               <  SRR19973081.2114710/150‑1 (MQ=60)
                                                      GTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCT                                                                                           <  SRR19973081.4295195/150‑1 (MQ=60)
                                                        ACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCT                                                                                         <  SRR19973081.609/150‑1 (MQ=60)
                                                          TGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCCGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCC                                                                                       >  SRR19973081.1731604/1‑150 (MQ=60)
                                                          TGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCC                                                                                       >  SRR19973081.1731652/1‑150 (MQ=60)
                                                            CACCGCCTACTGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTG                                                                                     <  SRR19973081.854222/150‑1 (MQ=60)
                                                            CACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTG                                                                                     >  SRR19973081.4325090/1‑150 (MQ=60)
                                                            CACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAACGTAAGTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTG                                                                                     <  SRR19973081.854220/150‑1 (MQ=60)
                                                            CACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTG                                                                                     <  SRR19973081.853872/150‑1 (MQ=60)
                                                             ACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGA                                                                                    <  SRR19973081.3054836/150‑1 (MQ=60)
                                                             ACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGA                                                                                    <  SRR19973081.4083371/150‑1 (MQ=60)
                                                               CGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCGGAAT                                                                                  >  SRR19973081.3709459/1‑150 (MQ=60)
                                                               CGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAAT                                                                                  >  SRR19973081.1502374/1‑150 (MQ=60)
                                                               CGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAAT                                                                                  >  SRR19973081.1502368/1‑150 (MQ=60)
                                                                 TCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTT                                                                                <  SRR19973081.941349/150‑1 (MQ=60)
                                                                 CCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTT                                                                                <  SRR19973081.4406021/150‑1 (MQ=60)
                                                                  CTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTA                                                                               <  SRR19973081.449520/150‑1 (MQ=60)
                                                                   TAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGGTACAAACATAATACTCTCTCCGGAATTTAT                                                                              >  SRR19973081.2452877/1‑150 (MQ=60)
                                                                   TAATGCCCCTGCACCCGCGCCGAGTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGTCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTAT                                                                              <  SRR19973081.2285439/150‑1 (MQ=60)
                                                                    AATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATG                                                                             >  SRR19973081.4166204/1‑150 (MQ=60)
                                                                      TGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGAT                                                                           <  SRR19973081.118698/150‑1 (MQ=60)
                                                                      TGCCCCCGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGCACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGAT                                                                           <  SRR19973081.118248/150‑1 (MQ=60)
                                                                         CCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCA                                                                        >  SRR19973081.4550779/1‑150 (MQ=60)
                                                                         CCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTAGTTACAAACATAATACTATCTCCAGAATTTATGATTCA                                                                        >  SRR19973081.4550768/1‑150 (MQ=60)
                                                                          CCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCAC                                                                       <  SRR19973081.3066492/150‑1 (MQ=60)
                                                                              CACCCGCGCCGATTGCGGTGTTGCTGTCCCGTTTAGACCAGTTAGACCAGGCACTCAGAGACATTTCCAAAGTAATGGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAA                                                                   <  SRR19973081.364629/150‑1 (MQ=60)
                                                                              CACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAA                                                                   <  SRR19973081.2445376/150‑1 (MQ=60)
                                                                              CACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAA                                                                   <  SRR19973081.2810638/150‑1 (MQ=60)
                                                                               ACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGACTTTATGATTCACGGAAG                                                                  >  SRR19973081.2509363/1‑150 (MQ=60)
                                                                                CCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGT                                                                 <  SRR19973081.1499510/150‑1 (MQ=60)
                                                                                CCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGT                                                                 <  SRR19973081.788784/150‑1 (MQ=60)
                                                                                 CCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTA                                                                <  SRR19973081.75017/150‑1 (MQ=60)
                                                                                 CCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTA                                                                <  SRR19973081.75061/150‑1 (MQ=60)
                                                                                       CGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCT                                                          >  SRR19973081.4420695/1‑150 (MQ=60)
                                                                                        GATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTC                                                         <  SRR19973081.1074971/150‑1 (MQ=60)
                                                                                        GATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTC                                                         <  SRR19973081.1717605/150‑1 (MQ=60)
                                                                                        GATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTC                                                         >  SRR19973081.2016051/1‑150 (MQ=60)
                                                                                        GATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTC                                                         <  SRR19973081.1717413/150‑1 (MQ=60)
                                                                                         ATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCA                                                        <  SRR19973081.2024611/150‑1 (MQ=60)
                                                                                         ATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCA                                                        <  SRR19973081.349823/150‑1 (MQ=60)
                                                                                          TTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTA                                                       <  SRR19973081.2816276/150‑1 (MQ=60)
                                                                                          TTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTA                                                       <  SRR19973081.2733725/150‑1 (MQ=60)
                                                                                           TGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATAATCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTAT                                                      >  SRR19973081.1659489/1‑150 (MQ=60)
                                                                                              GGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAAC                                                   <  SRR19973081.1010166/150‑1 (MQ=60)
                                                                                              GGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAAC                                                   <  SRR19973081.1605785/150‑1 (MQ=60)
                                                                                                 GTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTAT                                                <  SRR19973081.61680/150‑1 (MQ=60)
                                                                                                   TGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAA                                              >  SRR19973081.1551035/1‑150 (MQ=60)
                                                                                                    GCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAAT                                             >  SRR19973081.2625553/1‑150 (MQ=60)
                                                                                                    GCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAAT                                             >  SRR19973081.1422494/1‑150 (MQ=60)
                                                                                                    GCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAAT                                             >  SRR19973081.1421662/1‑150 (MQ=60)
                                                                                                     CGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATA                                            <  SRR19973081.1774853/150‑1 (MQ=60)
                                                                                                     CGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATA                                            <  SRR19973081.1773766/150‑1 (MQ=60)
                                                                                                     CGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATA                                            <  SRR19973081.1774694/150‑1 (MQ=60)
                                                                                                     CGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATA                                            <  SRR19973081.2165248/150‑1 (MQ=60)
                                                                                                     CGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATA                                            <  SRR19973081.2165123/150‑1 (MQ=60)
                                                                                                     CGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATA                                            <  SRR19973081.3377475/150‑1 (MQ=60)
                                                                                                        TCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCT                                         >  SRR19973081.709617/1‑150 (MQ=60)
                                                                                                         CCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTA                                        >  SRR19973081.4767713/1‑150 (MQ=60)
                                                                                                              TTAGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAAT                                   <  SRR19973081.935515/150‑1 (MQ=60)
                                                                                                                AGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAA                                 >  SRR19973081.4408673/1‑150 (MQ=60)
                                                                                                                AGACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCACTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAA                                 >  SRR19973081.1943812/1‑150 (MQ=60)
                                                                                                                 GACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAG                                <  SRR19973081.2293706/150‑1 (MQ=60)
                                                                                                                  ACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGT                               <  SRR19973081.2349682/150‑1 (MQ=60)
                                                                                                                  ACCAGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGT                               <  SRR19973081.4271490/150‑1 (MQ=60)
                                                                                                                     AGTTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTG                            >  SRR19973081.4509322/1‑150 (MQ=60)
                                                                                                                       TTAGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGT                          <  SRR19973081.2564337/150‑1 (MQ=60)
                                                                                                                        TAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTG                         <  SRR19973081.1351251/150‑1 (MQ=60)
                                                                                                                         AGAACAGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGA                        >  SRR19973081.996734/1‑150 (MQ=60)
                                                                                                                          GAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAA                       >  SRR19973081.1964447/1‑150 (MQ=60)
                                                                                                                              AGGCACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTA                   <  SRR19973081.3134524/150‑1 (MQ=60)
                                                                                                                                 CACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTC                >  SRR19973081.3065869/1‑150 (MQ=60)
                                                                                                                                 CACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTC                <  SRR19973081.3700600/150‑1 (MQ=60)
                                                                                                                                  ACTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCC               >  SRR19973081.4851476/1‑150 (MQ=60)
                                                                                                                                   CTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCG              >  SRR19973081.2560890/1‑150 (MQ=60)
                                                                                                                                   CTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCG              <  SRR19973081.2673975/150‑1 (MQ=60)
                                                                                                                                   CTCAGAGACATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCG              <  SRR19973081.3321343/150‑1 (MQ=60)
                                                                                                                                     CAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTG            <  SRR19973081.1531835/150‑1 (MQ=60)
                                                                                                                                     CAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTG            <  SRR19973081.3904400/150‑1 (MQ=60)
                                                                                                                                         GACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAAT        <  SRR19973081.1920802/150‑1 (MQ=60)
                                                                                                                                            ATTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTG     <  SRR19973081.4658437/150‑1 (MQ=60)
                                                                                                                                             TTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGC    >  SRR19973081.600619/1‑150 (MQ=60)
                                                                                                                                             TTTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGC    >  SRR19973081.3497603/1‑150 (MQ=60)
                                                                                                                                              TTCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCT   <  SRR19973081.3762993/150‑1 (MQ=60)
                                                                                                                                               TCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTC  <  SRR19973081.1374200/150‑1 (MQ=60)
                                                                                                                                               |                                                                                                                                                     
TAACACCTCCGACAGCTGCACCACCTAATGTACCCAACGTACTGCCATCGGTCAGTACTGCACCGCCTAATGCCCCTGCACCCGCGCCGATTGCGGTGTTGCGGTCCCGTTTAGACCAGTTAGAACAGGCACTCAGAGACATTGCCAAAGTAATTGCCAGAACAGCCGCGGTCATTTTTTTGCTCGTTACAAACATAATACTCTCTCCTGAATTTATGATTCACGGAAGTAAGCTCTCTATAACTATAATAGCTAAGAATAAGTCTGGTGAAATTATTCCGTGAAATCTGCTC  >  CP009273/1337390‑1337682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑