Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,832,308 G→C 8.2% S234T (AGT→ACT)  yicL → EamA family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,832,3080GC 8.2% 497.5 / 37.0 159S234T (AGT→ACT) yicLEamA family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base G (53/86);  new base C (5/8);  total (65/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.86e-01

Only 100 of 161 total aligned reads displayed.

CGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATT  >  CP009273/3832161‑3832453
                                                                                                                                                    |                                                                                                                                                  
cGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAg                                                                                                                                                    <  2:2150952/148‑1 (MQ=255)
 gTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGt                                                                                                                                                   <  2:147341/148‑1 (MQ=255)
 gTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGt                                                                                                                                                   <  1:1950159/148‑1 (MQ=255)
 gTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGt                                                                                                                                                   <  1:1950145/148‑1 (MQ=255)
   tACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTAAGTCCCAGACATGTAGTCt                                                                                                                                                 >  2:281643/1‑148 (MQ=255)
      cAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTa                                                                                                                                              <  1:3115026/148‑1 (MQ=255)
         tcgtcgGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCt                                                                                                                                           >  1:1214237/1‑148 (MQ=255)
         tcgtcgGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCt                                                                                                                                           <  1:1365398/148‑1 (MQ=255)
         tcgtcgGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCt                                                                                                                                           <  1:1365641/148‑1 (MQ=255)
             cgGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGaaa                                                                                                                                       <  2:2851845/148‑1 (MQ=255)
               gCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAgg                                                                                                                                     <  2:624285/148‑1 (MQ=255)
                cTGGAGTATGCTGAGTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGa                                                                                                                                    <  2:1975359/148‑1 (MQ=255)
                   gAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGca                                                                                                                                 <  1:1869544/148‑1 (MQ=255)
                   gAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGca                                                                                                                                 <  2:1212868/148‑1 (MQ=255)
                   gAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGAAATTTATTCTGTACCAGAAAGGAGca                                                                                                                                 >  1:2098837/1‑148 (MQ=255)
                      tATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑Caa                                                                                                                             <  2:667458/148‑1 (MQ=255)
                         gCTGATTGGCGGTCTGATTCTGTTGCCTTTTTCTGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑Caatta                                                                                                                          <  1:813445/148‑1 (MQ=255)
                         gCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTTTTTGTTGGTGATTGGTAAGTCCCTGACATTTATTGTGTACCTGAAAGGAGCA‑Caaaaa                                                                                                                          >  1:1446928/1‑145 (MQ=255)
                         gCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑Caatta                                                                                                                          <  1:1714519/148‑1 (MQ=255)
                         gCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCA‑Caatta                                                                                                                          <  2:2270122/148‑1 (MQ=255)
                          cTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑Caattaa                                                                                                                         <  1:1772619/148‑1 (MQ=255)
                          cTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑Caattaa                                                                                                                         <  1:1772514/148‑1 (MQ=255)
                          cTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑Caattaa                                                                                                                         <  1:2620313/148‑1 (MQ=255)
                          cTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑Caattaa                                                                                                                         <  2:3196321/148‑1 (MQ=255)
                          cTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑Caattaa                                                                                                                         <  1:791118/148‑1 (MQ=255)
                               tGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTGTTTTTTTTTTGTTGGGC‑TTTGTACATCCCCGAAAATTATTCTTTAAAAGAAAAGAGCA‑Caaaaaattttc                                                                                                                    >  1:1416427/1‑145 (MQ=255)
                               tGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTTTTTTGTTGTGATTTGTTCCTCCCAGACAATTATTATTTAACGGAAAAGAGCACCAATAAATTgc                                                                                                                     >  1:2237006/1‑147 (MQ=255)
                               tGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTTTTTTGTGGGGATTTGTTCGTCCCAGACAGTTAGTCTGTTACAGAAAGGAGCC‑CAAATAAATggt                                                                                                                    >  1:2237469/1‑147 (MQ=255)
                               tGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTTGTGGTCATTTGTACGTCCCAGAAATTTAGTATGTACCATAAACGAGCA‑CAAATAATTTGc                                                                                                                    >  1:2040384/1‑148 (MQ=255)
                                  cGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGTCATTTAGTCTGTACCAGAAAGGAGCA‑CAATTAATTGGCGGt                                                                                                                 >  2:2325695/1‑148 (MQ=255)
                                  cGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGt                                                                                                                 >  2:1832303/1‑148 (MQ=255)
                                  cGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGt                                                                                                                 <  2:2223675/148‑1 (MQ=255)
                                   ggTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTc                                                                                                                <  1:1384552/148‑1 (MQ=255)
                                       tGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCaaa                                                                                                            <  2:1916651/148‑1 (MQ=255)
                                         aTTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑GTTTTTATTTTGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTAACAGAAAGGAGCA‑CAAATAAATTGCGGTCCAAAAg                                                                                                          >  2:560625/1‑148 (MQ=255)
                                           tCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTTGCGGTCCAAAAGcc                                                                                                        >  2:3284051/1‑148 (MQ=255)
                                           tCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGcc                                                                                                        >  2:292969/1‑148 (MQ=255)
                                            cTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCa                                                                                                       <  1:672427/148‑1 (MQ=255)
                                                tGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTTCGTCCCTTACATTTAGTATGTACCTGAAAGGAGCA‑CAATTAATTTGCGGTCCAAAAGCCAGCAt                                                                                                   >  2:2794600/1‑148 (MQ=255)
                                                tGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACCGTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCAt                                                                                                   >  2:1493691/1‑148 (MQ=255)
                                                tGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCAt                                                                                                   <  1:770769/148‑1 (MQ=255)
                                                      tttATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAg                                                                                             <  2:19197/148‑1 (MQ=255)
                                                       ttATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGc                                                                                            <  2:3243112/148‑1 (MQ=255)
                                                         aTGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCtg                                                                                          <  2:2305167/148‑1 (MQ=255)
                                                         aTGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCtg                                                                                          <  1:902297/148‑1 (MQ=255)
                                                           gCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCtgtg                                                                                        <  1:1065689/148‑1 (MQ=255)
                                                           gCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCtgtg                                                                                        <  2:3023957/148‑1 (MQ=255)
                                                               gACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCCGCATTTTGAGCTGTGCAGa                                                                                    >  2:433048/1‑148 (MQ=255)
                                                               gACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGa                                                                                    <  2:1432915/148‑1 (MQ=255)
                                                                aCAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTTCCTGAAAGGAGCC‑CAATTAATTGGCGGTCCCAAAGCCCGCATTTTGAGCTGTGCAGaa                                                                                   >  2:917901/1‑148 (MQ=255)
                                                                aCAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTTAAAGGAGCA‑CAAATAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGaa                                                                                   >  2:328233/1‑148 (MQ=255)
                                                                 cAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAAc                                                                                  <  1:3114441/148‑1 (MQ=255)
                                                                  aaGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAAcc                                                                                 <  2:2349832/148‑1 (MQ=255)
                                                                   aGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCa                                                                                <  2:1769241/148‑1 (MQ=255)
                                                                    ggAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGGGATTTGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGATCA‑CAATTAATTTGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCAt                                                                               >  1:3269756/1‑148 (MQ=255)
                                                                      aaCAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTTGGGGGCAATGGTACGGCCCGGACATTTAGTGTGTACCGGAAAGGAGCA‑CCAATAAGTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTa                                                                             >  1:1091784/1‑148 (MQ=255)
                                                                      aaCAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTa                                                                             <  2:2196400/148‑1 (MQ=255)
                                                                       aCAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTaa                                                                            >  2:2874843/1‑148 (MQ=255)
                                                                         aaaCTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTTGTGGTCATTGGTACGTCCCTGACCTTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGt                                                                          >  2:1751062/1‑148 (MQ=255)
                                                                         aaaCTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAAATAATTGGCGGTCCAAAAGCCAGCATTTTGAGCAGTGCAGAACCCTTAAgg                                                                          >  2:1751056/1‑147 (MQ=255)
                                                                           aCTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAg                                                                        >  2:3249858/1‑148 (MQ=255)
                                                                            cTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTatc                                                                       >  2:1364569/1‑146 (MQ=255)
                                                                               tGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAgcgcg                                                                    <  2:945368/148‑1 (MQ=255)
                                                                                  cGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTa                                                                 <  1:1668323/148‑1 (MQ=255)
                                                                                  cGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTa                                                                 <  2:1856423/148‑1 (MQ=255)
                                                                                     tAACGGCAGTTTGATTCTGGCGT‑TTTTTTTTTTTGTGGTCATTTGTACGTCCCAGACATTTATTGTGTACCGGAAAGGAGCA‑CAAATAAATTGCGGTCCAAAAACCCCCATTTTTAGCTGGGCAGAACCATTAAGTATCGCGCTaagc                                                              >  1:2326061/1‑145 (MQ=255)
                                                                                     tAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAAATGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTActc                                                              >  2:1624294/1‑148 (MQ=255)
                                                                                      aaCGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTActct                                                             <  1:2387892/148‑1 (MQ=255)
                                                                                       aCGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTActctc                                                            <  1:3230193/148‑1 (MQ=255)
                                                                                             gTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTTGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgct                                                      >  2:1598074/1‑148 (MQ=255)
                                                                                              tttGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgctg                                                     <  2:909262/148‑1 (MQ=255)
                                                                                                 gATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgctgttg                                                  <  2:407622/148‑1 (MQ=255)
                                                                                                  aTTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgctgttgc                                                 <  1:2906602/148‑1 (MQ=255)
                                                                                                        gCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGa                                           <  2:348470/148‑1 (MQ=255)
                                                                                                         cGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTTCTGGGGAt                                          >  2:2644571/1‑148 (MQ=255)
                                                                                                         cGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGAt                                          <  2:2275603/148‑1 (MQ=255)
                                                                                                         cGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACAATTATTCTGTACCTGAAAGGAGCA‑CAATTAATTTGCGGTCCAAAAGCCCGCCTTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGGTGTTGGTGGGGAt                                          >  1:2862016/1‑148 (MQ=255)
                                                                                                              tttttATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACg                                      <  2:1366829/148‑1 (MQ=255)
                                                                                                                    tttGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTcaca                                <  1:2511826/148‑1 (MQ=255)
                                                                                                                        gtggtCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTAc                            >  2:1696688/1‑148 (MQ=255)
                                                                                                                         tggtCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTAcc                           >  2:20083/1‑148 (MQ=255)
                                                                                                                          ggtCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCg                          >  1:334503/1‑148 (MQ=255)
                                                                                                                           gtCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCgg                         <  1:52162/148‑1 (MQ=255)
                                                                                                                           gtCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCgg                         >  2:110571/1‑148 (MQ=255)
                                                                                                                           gtCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACAATACCgg                         >  2:110639/1‑148 (MQ=255)
                                                                                                                             cATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGAc                       >  1:1998953/1‑148 (MQ=255)
                                                                                                                                tGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActgg                    <  2:1063683/148‑1 (MQ=255)
                                                                                                                                 ggTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActggc                   <  1:2875129/148‑1 (MQ=255)
                                                                                                                                      gTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGa              <  2:1510116/148‑1 (MQ=255)
                                                                                                                                      gTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGa              <  2:1510110/148‑1 (MQ=255)
                                                                                                                                       tCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGaa             <  1:958661/148‑1 (MQ=255)
                                                                                                                                          cTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgc          <  2:2040384/148‑1 (MQ=255)
                                                                                                                                            gACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctg        >  1:2377442/1‑148 (MQ=255)
                                                                                                                                            gACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctg        >  1:2826103/1‑148 (MQ=255)
                                                                                                                                            gACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctg        >  1:1338001/1‑148 (MQ=255)
                                                                                                                                            gACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctg        >  1:1337429/1‑148 (MQ=255)
                                                                                                                                             aCATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctgc       <  1:2706487/148‑1 (MQ=255)
                                                                                                                                             aCATTTACTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctgc       >  2:1151991/1‑148 (MQ=255)
                                                                                                                                             aCATTTACTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctgc       >  2:1151979/1‑148 (MQ=255)
                                                                                                                                                 ttAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGAt   <  1:2950557/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGT‑TTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCA‑CAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATT  >  CP009273/3832161‑3832453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A