Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,832,308 G→C S234T (AGT→ACT)  yicL → EamA family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,832,3080GC98.1% 796.7 / NA 209S234T (AGT→ACT) yicLEamA family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (104/101);  minor base A (4/0);  total (108/101)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

TTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGA  >  CP009273/3832163‑3832451
                                                                                                                                                 |                                                                                                                                               
  aCCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTa                                                                                                                                           >  2:3172468/1‑150 (MQ=255)
    cAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTAcc                                                                                                                                         <  1:306646/150‑1 (MQ=255)
      gtcgtcGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTg                                                                                                                                       <  2:3629423/150‑1 (MQ=255)
          tcgGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAg                                                                                                                                   <  2:905963/150‑1 (MQ=255)
          tcgGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAg                                                                                                                                   <  2:3274337/150‑1 (MQ=255)
           cgacTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAgg                                                                                                                                  >  2:1868870/4‑150 (MQ=255)
             gCTGGAGTATGCTGATTGGCGTTATGTTTATGTTGCCTTTTTATGCCAGACATGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTTTTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGTAg                                                                                                                                <  2:1552290/150‑1 (MQ=255)
             gCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAg                                                                                                                                <  2:1164098/150‑1 (MQ=255)
              cTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCAGACATTTACTCTGTACCTGAAAAGAGc                                                                                                                               >  1:1637786/1‑150 (MQ=255)
               tGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACATTTACTCTGTACCTGAAATGAGca                                                                                                                              >  1:2861562/1‑150 (MQ=255)
               tGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGACGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTACCTGACATTTAATCTGTACCTGAAATGAGca                                                                                                                              >  1:2861809/1‑150 (MQ=255)
                ggAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGcac                                                                                                                             <  2:746013/150‑1 (MQ=255)
                  aGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaa                                                                                                                           >  2:203171/1‑150 (MQ=255)
                   gTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaat                                                                                                                          >  2:687671/1‑150 (MQ=255)
                    tATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaatt                                                                                                                         >  1:445517/1‑150 (MQ=255)
                    tATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaatt                                                                                                                         <  2:2463956/150‑1 (MQ=255)
                     aTGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaatta                                                                                                                        <  1:1484952/150‑1 (MQ=255)
                     aTGCTGATTGGCGGTCTGATTCTGTTGCATTTTTATTCCAGACAAGGAAAAAAATTTGTCGTTAACGGCAGTTTTATTCTGGCGTTTTTTTATTTGGTTGTCATTGGTAAGTACCTGACATTTACTCTGTAACTGAAATGAGCACaatta                                                                                                                        >  2:1002808/1‑150 (MQ=255)
                        cTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaattaatt                                                                                                                     <  2:1818881/150‑1 (MQ=255)
                         tGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTg                                                                                                                    <  2:3553510/150‑1 (MQ=255)
                           aTTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGc                                                                                                                  <  1:2712770/150‑1 (MQ=255)
                              ggCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTTGCGGt                                                                                                               >  1:3041509/1‑150 (MQ=255)
                                 ggTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCa                                                                                                            >  2:2566907/1‑150 (MQ=255)
                                 ggTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCa                                                                                                            <  1:2622720/150‑1 (MQ=255)
                                 ggTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCa                                                                                                            >  2:3400185/1‑150 (MQ=255)
                                 ggTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAAGAGCACAATTAATTTGCGGTCCa                                                                                                            >  1:1595156/1‑150 (MQ=255)
                                  gTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAAATAATTTGCGGTCCaa                                                                                                           >  1:3233723/1‑150 (MQ=255)
                                  gTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAAAGGCAGTTTGATTCTGGCGTTTTTTTATTTTGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCaa                                                                                                           >  2:2397649/1‑150 (MQ=255)
                                       aTTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGcc                                                                                                      <  2:572387/150‑1 (MQ=255)
                                       aTTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGcc                                                                                                      <  2:2173594/150‑1 (MQ=255)
                                         tCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAg                                                                                                    >  2:908069/1‑150 (MQ=255)
                                                 cTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGa                                                                                            >  2:2856232/1‑150 (MQ=255)
                                                  tttttATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCAGACAATTACACTGTACCAGAAAGGAGCACAATTAATTGTCGGTCCAAAATCCAGCATTTTGAg                                                                                           >  1:35773/1‑150 (MQ=255)
                                                   ttttATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGc                                                                                          >  2:729369/1‑150 (MQ=255)
                                                   ttttATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGc                                                                                          <  1:1409818/150‑1 (MQ=255)
                                                       aTGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACCTTTACTCAGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCtgtg                                                                                      >  1:2071206/1‑150 (MQ=255)
                                                         gCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCa                                                                                    <  2:1190461/150‑1 (MQ=255)
                                                          ccAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAg                                                                                   >  2:1995112/1‑150 (MQ=255)
                                                          ccAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAg                                                                                   >  2:84421/1‑150 (MQ=255)
                                                          ccAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAg                                                                                   <  1:797539/150‑1 (MQ=255)
                                                           cAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGa                                                                                  <  1:1025684/150‑1 (MQ=255)
                                                           cAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGa                                                                                  >  1:2174487/1‑150 (MQ=255)
                                                           cAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGa                                                                                  <  1:693374/150‑1 (MQ=255)
                                                            aGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGaa                                                                                 >  2:499859/1‑150 (MQ=255)
                                                              aCAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAAcc                                                                               <  1:2258858/150‑1 (MQ=255)
                                                              aCAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAAcc                                                                               >  2:3379846/1‑150 (MQ=255)
                                                              aCAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAAcc                                                                               <  1:3643692/150‑1 (MQ=255)
                                                              aCAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAAcc                                                                               <  1:3549966/150‑1 (MQ=255)
                                                                aaGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCAt                                                                             >  1:2128348/1‑150 (MQ=255)
                                                                aaGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCAt                                                                             >  1:1820149/1‑150 (MQ=255)
                                                                 aGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCAtt                                                                            >  2:2246399/1‑150 (MQ=255)
                                                                  ggAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTa                                                                           <  1:2744514/150‑1 (MQ=255)
                                                                    aaCAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAg                                                                         <  2:1071576/150‑1 (MQ=255)
                                                                    aaCAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAg                                                                         >  2:1191022/1‑150 (MQ=255)
                                                                      cAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTa                                                                       <  2:1436202/150‑1 (MQ=255)
                                                                      cAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTa                                                                       <  2:1626990/150‑1 (MQ=255)
                                                                       aaaCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAg                                                                      <  2:1258040/150‑1 (MQ=255)
                                                                       aaaCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAg                                                                      >  2:3251026/1‑150 (MQ=255)
                                                                       aaaCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCAGAAAGGAGCACAAATAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAg                                                                      >  1:839315/1‑150 (MQ=255)
                                                                        aaCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAgc                                                                     <  1:937613/150‑1 (MQ=255)
                                                                          cTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAACCAGCATTTTGAGCTGTGCAGAACCATTAAGTAtcgc                                                                   >  1:2487944/1‑150 (MQ=255)
                                                                               tCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTAc                                                              >  2:2258238/1‑150 (MQ=255)
                                                                                cGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTAct                                                             <  1:1233489/150‑1 (MQ=255)
                                                                                cGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACAATTACTCTGTACCTGAAAGGAGCACAATTAATTTGCGGTCCAAAATCCAGCATTTTGAGCTGTGCAGAACCATTAAGTATCGCGCTAct                                                             >  1:1316772/1‑150 (MQ=255)
                                                                                   tAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTActctc                                                          >  2:777979/1‑150 (MQ=255)
                                                                                    aaCGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTTCTCTGTTCCTGATAGGAGCTCTATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTActctct                                                         <  1:1729585/150‑1 (MQ=255)
                                                                                    aaCGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTActctct                                                         <  2:2618653/150‑1 (MQ=255)
                                                                                      cGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCttt                                                       <  1:1601434/150‑1 (MQ=255)
                                                                                      cGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCttt                                                       <  1:2858460/150‑1 (MQ=255)
                                                                                         cAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgct                                                    <  1:3063656/150‑1 (MQ=255)
                                                                                         cAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgct                                                    <  2:3522470/150‑1 (MQ=255)
                                                                                         cAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgct                                                    >  2:221502/1‑150 (MQ=255)
                                                                                              tGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACAATTAAACTGTACCATAAAAGAGCCCAAATAATTTTCGGTCCAAAAACCAACAATTTTATCTGTGCAAAACCAATAAATAACGCGCTACACTCTttgctgttgc                                               >  1:2239280/1‑150 (MQ=255)
                                                                                               gATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgatgttgat                                              >  2:1957154/1‑148 (MQ=255)
                                                                                                 ttCTGGCGTTTTTTTATTTGGTTGTCATTGGTACGTCCCTGACCTTTACTCTGTACCTGAAAGGAGCACAATTAATTTGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAATAAGAGCGCTACTCTCATTGCTGTTGATgg                                            >  2:1929217/1‑150 (MQ=255)
                                                                                                 ttCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACATTTACTCTGTACCTGAAAAGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTTCATAACCATTAATTAGCGCGCTACTCTCTTTTCTGTTGCTgg                                            >  1:2313024/1‑150 (MQ=255)
                                                                                                   cTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTgggg                                          >  1:3095799/1‑150 (MQ=255)
                                                                                                    tGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGa                                         <  1:687620/150‑1 (MQ=255)
                                                                                                       cGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCa                                      >  1:144543/1‑150 (MQ=255)
                                                                                                          ttttttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCTCGCTACTCTCTTTGATGTTGCTGGGGATCACGt                                   >  2:1438922/1‑150 (MQ=255)
                                                                                                           tttttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGtt                                  >  2:2626395/1‑150 (MQ=255)
                                                                                                            ttttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTc                                 >  1:1286720/1‑150 (MQ=255)
                                                                                                             tttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTca                                <  1:186157/150‑1 (MQ=255)
                                                                                                              ttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTcac                               <  2:2950283/150‑1 (MQ=255)
                                                                                                                 tttGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACAtt                            <  1:1759576/150‑1 (MQ=255)
                                                                                                                   tggtggTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTAc                          >  2:1504145/1‑150 (MQ=255)
                                                                                                                         tCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGAct                    <  1:1846315/150‑1 (MQ=255)
                                                                                                                           aTTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActgg                  >  2:2955592/1‑150 (MQ=255)
                                                                                                                           aTTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActgg                  >  1:3725203/1‑150 (MQ=255)
                                                                                                                            ttGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActggc                 >  1:1296131/1‑150 (MQ=255)
                                                                                                                                tACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTggg             <  1:3400185/150‑1 (MQ=255)
                                                                                                                                  cGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGaa           >  1:1271804/1‑150 (MQ=255)
                                                                                                                                   gTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAc          >  2:2113220/1‑150 (MQ=255)
                                                                                                                                   gTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAc          >  2:713711/1‑150 (MQ=255)
                                                                                                                                   gTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAc          >  1:2872101/1‑150 (MQ=255)
                                                                                                                                     cccTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgc        <  2:445517/150‑1 (MQ=255)
                                                                                                                                      ccTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgct       <  2:1490246/150‑1 (MQ=255)
                                                                                                                                      ccTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgct       >  1:2005992/1‑150 (MQ=255)
                                                                                                                                       cTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctg      >  1:348264/1‑150 (MQ=255)
                                                                                                                                       cTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctg      >  1:2687156/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGA  >  CP009273/3832163‑3832451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATTCTG  >  CP009273/3832159‑3832456
                                                                                                                                                     |                                                                                                                                                    
CACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAC                                                                                                                                                      >  SRR19973061.3531494/1‑150 (MQ=60)
       CCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTGGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTAC                                                                                                                                               <  SRR19973061.269740/150‑1 (MQ=60)
        CAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACC                                                                                                                                              <  SRR19973061.307451/150‑1 (MQ=60)
         AGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCT                                                                                                                                             <  SRR19973061.3453196/150‑1 (MQ=60)
          GTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTG                                                                                                                                            <  SRR19973061.2338074/150‑1 (MQ=60)
            CGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAA                                                                                                                                          <  SRR19973061.1448476/150‑1 (MQ=60)
            CGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAA                                                                                                                                          <  SRR19973061.2986562/150‑1 (MQ=60)
              TCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAG                                                                                                                                        <  SRR19973061.76328/150‑1 (MQ=60)
                GGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGA                                                                                                                                      >  SRR19973061.1261151/1‑150 (MQ=60)
                  CTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCAGACATTTACTCTGTACCTGAAAAGAGC                                                                                                                                    >  SRR19973061.1641887/1‑150 (MQ=60)
                   TGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACATTTACTCTGTACCTGAAATGAGCA                                                                                                                                   >  SRR19973061.2868890/1‑150 (MQ=60)
                   TGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCC                                                                                                                                   >  SRR19973061.2868913/1‑150 (MQ=60)
                   TGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGACGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTACCTGACATTTAATCTGTACCTGAAATGAGCA                                                                                                                                   >  SRR19973061.2869138/1‑150 (MQ=60)
                    GGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCAC                                                                                                                                  <  SRR19973061.3575442/150‑1 (MQ=60)
                        TATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATT                                                                                                                              >  SRR19973061.446727/1‑150 (MQ=60)
                        TATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCAGACATTTACTCTGTACCTGAAATGAGCCCAATT                                                                                                                              >  SRR19973061.1483232/1‑150 (MQ=60)
                         ATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTA                                                                                                                             <  SRR19973061.1488677/150‑1 (MQ=60)
                          TGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAG‑TTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAAT                                                                                                                           <  SRR19973061.2867951/150‑1 (MQ=60)
                            CTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATT                                                                                                                          <  SRR19973061.909906/150‑1 (MQ=60)
                              GATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGG                                                                                                                        <  SRR19973061.2174463/150‑1 (MQ=60)
                               ATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGC                                                                                                                       <  SRR19973061.809984/150‑1 (MQ=60)
                               ATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGC                                                                                                                       <  SRR19973061.2719756/150‑1 (MQ=60)
                                TTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCG                                                                                                                      <  SRR19973061.3748616/150‑1 (MQ=60)
                                  GGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTTGCGGT                                                                                                                    >  SRR19973061.3049375/1‑150 (MQ=60)
                                   GCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTC                                                                                                                   <  SRR19973061.3610336/150‑1 (MQ=60)
                                    CGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCC                                                                                                                  <  SRR19973061.1338558/150‑1 (MQ=60)
                                     GGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCA                                                                                                                 <  SRR19973061.2629458/150‑1 (MQ=60)
                                     GGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAAGAGCACAATTAATTTGCGGTCCA                                                                                                                 >  SRR19973061.1599116/1‑150 (MQ=60)
                                     GGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCAGACATTTACTCTGTACCTGAAATGAGCACAAATAATTGGCGGTCCA                                                                                                                 >  SRR19973061.3499590/1‑150 (MQ=60)
                                      GTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAAATAATTTGCGGTCCAA                                                                                                                >  SRR19973061.3242032/1‑150 (MQ=60)
                                            TTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGTCGTTTTATTATTTGGTTGTCATTGGTACGTCCCTGACATTTACTCAGTACCTGAAAGGAGCCCAAATAATTTGCGTTCCAAAATCCA                                                                                                          >  SRR19973061.3101494/1‑150 (MQ=60)
                                            TTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGTAGCACAATTAATTGTCGGTCCAAAAGCCA                                                                                                          >  SRR19973061.2107389/1‑150 (MQ=60)
                                                   GCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACAACCCAGACATTTACACAGAACCATAAAGGAACACAAATAATTGGCGGTCCAAAAACCAACAATTT                                                                                                   >  SRR19973061.185658/1‑150 (MQ=60)
                                                     CTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGA                                                                                                 <  SRR19973061.314230/150‑1 (MQ=60)
                                                      TTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCAGACAATTACACTGTACCAGAAAGGAGCACAATTAATTGTCGGTCCAAAATCCAGCATTTTGAG                                                                                                >  SRR19973061.35907/1‑150 (MQ=60)
                                                       TTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGC                                                                                               <  SRR19973061.1413376/150‑1 (MQ=60)
                                                           ATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACCTTTACTCAGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTG                                                                                           >  SRR19973061.2076409/1‑150 (MQ=60)
                                                           ATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTG                                                                                           >  SRR19973061.1226499/1‑150 (MQ=60)
                                                              CCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACACTTAAATGGCGGTCCAAAAGCCAGCAATTTGAGCggggccg                                                                                        >  SRR19973061.999764/1‑143 (MQ=60)
                                                              CCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAG                                                                                        <  SRR19973061.799630/150‑1 (MQ=60)
                                                               CAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGA                                                                                       >  SRR19973061.2179952/1‑150 (MQ=60)
                                                               CAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGA                                                                                       <  SRR19973061.695175/150‑1 (MQ=60)
                                                               CAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGA                                                                                       <  SRR19973061.1028307/150‑1 (MQ=60)
                                                                  ACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACC                                                                                    <  SRR19973061.3653003/150‑1 (MQ=60)
                                                                  ACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACC                                                                                    <  SRR19973061.3559053/150‑1 (MQ=60)
                                                                  ACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACC                                                                                    <  SRR19973061.2264546/150‑1 (MQ=60)
                                                                  ACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACC                                                                                    <  SRR19973061.2265279/150‑1 (MQ=60)
                                                                    AAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCAT                                                                                  >  SRR19973061.2133691/1‑150 (MQ=60)
                                                                    AAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCAT                                                                                  >  SRR19973061.3724512/1‑150 (MQ=60)
                                                                    AAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCAT                                                                                  >  SRR19973061.1824708/1‑150 (MQ=60)
                                                                     AGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGATCACAATTAATTGGCGGTCCAAAAGCCAACCTTTTGAGCTGTGCAGAACCATT                                                                                 >  SRR19973061.2681228/1‑150 (MQ=60)
                                                                      GGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTA                                                                                <  SRR19973061.2751575/150‑1 (MQ=60)
                                                                         ACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGT                                                                             >  SRR19973061.1821958/1‑150 (MQ=60)
                                                                           AAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAAGAGCACAATTAATTTGCGGTCCAAAATCCAGCATTTTTAGCTGTGCAGAACCATTAAGTAG                                                                           >  SRR19973061.2249457/1‑150 (MQ=60)
                                                                           AAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCAGAAAGGAGCCCAAATAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAG                                                                           >  SRR19973061.841500/1‑150 (MQ=60)
                                                                            AACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGC                                                                          <  SRR19973061.939997/150‑1 (MQ=60)
                                                                              CTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAACCAGCATTTTGAGCTGTGCAGAACCATTAAGTATCGC                                                                        >  SRR19973061.2494281/1‑150 (MQ=60)
                                                                                  GTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTA                                                                    <  SRR19973061.1280539/150‑1 (MQ=60)
                                                                                    CGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACT                                                                  <  SRR19973061.2625189/150‑1 (MQ=60)
                                                                                    CGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACT                                                                  <  SRR19973061.1236554/150‑1 (MQ=60)
                                                                                    CGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACAATTACTCTGTACCTGAAAGGAGCACAATTAATTTGCGGTCCAAAATCCAGCATTTTGAGCTGTGCAGAACCATTAAGTATCGCGCTACT                                                                  >  SRR19973061.1320081/1‑150 (MQ=60)
                                                                                     GTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACCTTTACTCTGTACCTGAAAAGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTATCGCGCTACTC                                                                 >  SRR19973061.1649283/1‑150 (MQ=60)
                                                                                       TAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAATCTGTACCTGAAAAGAGCACAATTAATTGGCGGTCCAAAAACCAGCATTTTGAGCTGTGCAGAACCATTAATTAGCGCGCTACTCTC                                                               >  SRR19973061.1311132/1‑150 (MQ=60)
                                                                                        AACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTTCTCTGTTCCTGATAGGAGCTCTATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCT                                                              <  SRR19973061.1733928/150‑1 (MQ=60)
                                                                                        AACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCT                                                              <  SRR19973061.1733880/150‑1 (MQ=60)
                                                                                          CGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTT                                                            <  SRR19973061.1605416/150‑1 (MQ=60)
                                                                                          CGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTT                                                            <  SRR19973061.2865777/150‑1 (MQ=60)
                                                                                          CGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTT                                                            <  SRR19973061.538448/150‑1 (MQ=60)
                                                                                             CAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCT                                                         <  SRR19973061.3071567/150‑1 (MQ=60)
                                                                                             CAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCT                                                         <  SRR19973061.3071544/150‑1 (MQ=60)
                                                                                               GTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGT                                                       <  SRR19973061.3073303/150‑1 (MQ=60)
                                                                                                TTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTT                                                      <  SRR19973061.2272869/150‑1 (MQ=60)
                                                                                                  TGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACAATTAAACTGTACCATAAAAGAGCCCAAATAATTTTCGGTCCAAAAACCAACAATTTTATCTGTGCAAAACCAATAAATAACGCGCTACACTCTTTGCTGTTGC                                                    >  SRR19973061.2244906/1‑150 (MQ=31)
                                                                                                     TTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACATTTACTCTGTACCTGAAAAGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTTCATAACCATTAATTAGCGCGCTACTCTCTTTTCTGTTGCTGG                                                 >  SRR19973061.2318889/1‑150 (MQ=60)
                                                                                                      TCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACATTTACTCTGTACCAGAAAAGATCACAAATAATTGGCGGTCCAAAAACCAGCAATTTTATCTGTTCAAAACCAATAAGTATCGCGCTACTCTCTTTGCTGTTGCTGGG                                                >  SRR19973061.2241949/1‑150 (MQ=60)
                                                                                                       CTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGG                                               >  SRR19973061.3103775/1‑150 (MQ=60)
                                                                                                        TGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGA                                              <  SRR19973061.689401/150‑1 (MQ=60)
                                                                                                        TGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGA                                              <  SRR19973061.689452/150‑1 (MQ=60)
                                                                                                         GGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGAT                                             <  SRR19973061.2874579/150‑1 (MQ=60)
                                                                                                           CGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCA                                           >  SRR19973061.145007/1‑150 (MQ=60)
                                                                                                                TTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTC                                      >  SRR19973061.1289909/1‑150 (MQ=60)
                                                                                                                 TTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCA                                     <  SRR19973061.186721/150‑1 (MQ=60)
                                                                                                                  TTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCAC                                    <  SRR19973061.129745/150‑1 (MQ=60)
                                                                                                                  TTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCAC                                    >  SRR19973061.1610882/1‑150 (MQ=60)
                                                                                                                    ATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACAT                                  <  SRR19973061.502642/150‑1 (MQ=60)
                                                                                                                     TTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATT                                 <  SRR19973061.3363038/150‑1 (MQ=60)
                                                                                                                     TTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATT                                 <  SRR19973061.1763344/150‑1 (MQ=60)
                                                                                                                     TTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATT                                 <  SRR19973061.1763983/150‑1 (MQ=60)
                                                                                                                             TCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACT                         <  SRR19973061.1850932/150‑1 (MQ=60)
                                                                                                                             TCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACT                         <  SRR19973061.1850933/150‑1 (MQ=60)
                                                                                                                               ATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGG                       >  SRR19973061.3734724/1‑150 (MQ=60)
                                                                                                                                TTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGC                      >  SRR19973061.1299355/1‑150 (MQ=60)
                                                                                                                                 TGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCGACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCT                     <  SRR19973061.2857119/150‑1 (MQ=60)
                                                                                                                                  GGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTG                    >  SRR19973061.1991034/1‑150 (MQ=60)
                                                                                                                                   GTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGG                   >  SRR19973061.2747238/1‑150 (MQ=60)
                                                                                                                                    TACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGG                  <  SRR19973061.3408915/150‑1 (MQ=60)
                                                                                                                                      CGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAA                >  SRR19973061.1274937/1‑150 (MQ=60)
                                                                                                                                       GTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAC               >  SRR19973061.2879465/1‑150 (MQ=60)
                                                                                                                                          CCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCT            >  SRR19973061.1507855/1‑150 (MQ=60)
                                                                                                                                          CCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCT            >  SRR19973061.2011661/1‑150 (MQ=60)
                                                                                                                                          CCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCT            >  SRR19973061.2011023/1‑150 (MQ=60)
                                                                                                                                          CCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCT            >  SRR19973061.3061685/1‑150 (MQ=60)
                                                                                                                                           CTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGACCGCTG           >  SRR19973061.349196/1‑150 (MQ=60)
                                                                                                                                           CTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTG           >  SRR19973061.2694068/1‑150 (MQ=60)
                                                                                                                                                   TACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATTCT   <  SRR19973061.768609/150‑1 (MQ=60)
                                                                                                                                                    ACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATTCTG  >  SRR19973061.1188976/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CACGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATTCTG  >  CP009273/3832159‑3832456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑