Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,832,308 G→C 12.7% S234T (AGT→ACT)  yicL → EamA family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,832,3080GC12.7% 532.0 / 67.8 179S234T (AGT→ACT) yicLEamA family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base G (63/89);  new base C (10/13);  total (77/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.74e-01

Only 100 of 182 total aligned reads displayed.

CGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATT  >  CP009273/3832161‑3832453
                                                                                                                                                   |                                                                                                                                                 
cGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAg                                                                                                                                                   <  2:3338139/148‑1 (MQ=255)
 gTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGt                                                                                                                                                  <  2:988411/148‑1 (MQ=255)
    aCCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTATGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTAGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGGTGTCATTTTTAAGTACCTGTCATTTTGtatt                                                                                                                                               >  2:2402737/1‑145 (MQ=255)
        gtcgtcGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTAcc                                                                                                                                           <  1:2235763/148‑1 (MQ=255)
        gtcgtcGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTAcc                                                                                                                                           <  2:1630337/148‑1 (MQ=255)
         tcgtcgGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTTGTCATTTGTAAGTCCCTGACAATTAGTATGTAACt                                                                                                                                          >  1:1972099/1‑148 (MQ=255)
              ggCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTGATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAg                                                                                                                                     <  2:2784422/148‑1 (MQ=255)
                cTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGa                                                                                                                                   >  2:2736295/1‑148 (MQ=255)
                cTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGa                                                                                                                                   <  2:532438/148‑1 (MQ=255)
                 tGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAg                                                                                                                                  <  1:30342/148‑1 (MQ=255)
                 tGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAg                                                                                                                                  <  2:840344/148‑1 (MQ=255)
                 tGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAg                                                                                                                                  <  1:2411873/148‑1 (MQ=255)
                  ggAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGc                                                                                                                                 <  2:2373977/148‑1 (MQ=255)
                   gAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCCTTGGTACGTCCCTGACCTTTAGTCTGTACCGGAAAGGAGcc                                                                                                                                >  1:3158164/1‑147 (MQ=255)
                   gAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGca                                                                                                                                >  1:3538871/1‑148 (MQ=255)
                   gAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCGGAAAGGATca                                                                                                                                >  2:2618191/1‑148 (MQ=255)
                   gAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCGGAGATTTAGTCTGTACCTGAAAGGAGca                                                                                                                                >  1:1980708/1‑148 (MQ=255)
                     gTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACCTTTAGTCTGTACCTGAAAGGAGcaca                                                                                                                              >  1:2886777/1‑148 (MQ=255)
                      tATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACaa                                                                                                                             <  2:3179300/148‑1 (MQ=255)
                      tATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACaa                                                                                                                             <  1:100806/148‑1 (MQ=255)
                       aTGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACaat                                                                                                                            <  2:189994/148‑1 (MQ=255)
                       aTGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACaat                                                                                                                            <  2:148487/148‑1 (MQ=255)
                           tGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACaattaat                                                                                                                        <  2:391183/148‑1 (MQ=255)
                             aTTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTg                                                                                                                      <  2:1774132/148‑1 (MQ=255)
                               tGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGc                                                                                                                    <  1:3090791/148‑1 (MQ=255)
                                 gCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCgg                                                                                                                  >  2:786945/1‑148 (MQ=255)
                                          ttCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGc                                                                                                         <  2:3092750/148‑1 (MQ=255)
                                          ttCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGc                                                                                                         <  2:2165731/148‑1 (MQ=255)
                                          ttCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGc                                                                                                         <  1:588109/148‑1 (MQ=255)
                                           tCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGcc                                                                                                        <  2:427526/148‑1 (MQ=255)
                                           tCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTGTTTATTTGGTGGTCATTGGTACGTCCCGGACATTTACTCTGGAACTGAAAGGAGCACAATTAATTGGCGGTCCAAAAAcc                                                                                                        >  1:1895094/1‑148 (MQ=255)
                                            cTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTTTTTTGTGGTGCTTTGTAAGTCCCAGAAAATTATTGTGTAACAGAAAATAGCACAAATAATTTGGGGTGCaaaaaaaa                                                                                                       >  1:3294154/1‑144 (MQ=255)
                                              gTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGc                                                                                                     <  2:3059150/148‑1 (MQ=255)
                                               ttGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCCTTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAAATAATTTGGGGTCCAAAAGCCAGCa                                                                                                    >  1:853662/1‑148 (MQ=255)
                                               ttGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCa                                                                                                    >  1:2655376/1‑148 (MQ=255)
                                                tGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCAt                                                                                                   <  1:3432847/148‑1 (MQ=255)
                                                 gCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTTGCGGTCCAAAAGCCCGCAtt                                                                                                  >  1:2674479/1‑148 (MQ=255)
                                                  ccTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCCTTGGTACGTCCCTGACCTTTAGGCTGTACCTGAAAGGAGCACCATTAATTGGCGGTCCAAAAGCCAGCAttt                                                                                                 >  2:100172/1‑148 (MQ=255)
                                                  ccTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCAttt                                                                                                 <  2:742855/148‑1 (MQ=255)
                                                  ccTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCAttt                                                                                                 >  2:2068761/1‑148 (MQ=255)
                                                  ccTTTGTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCAttt                                                                                                 <  1:1989691/148‑1 (MQ=255)
                                                       ttATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGc                                                                                            <  2:2705873/148‑1 (MQ=255)
                                                       ttATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGc                                                                                            <  2:2705981/148‑1 (MQ=255)
                                                       ttATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGc                                                                                            <  2:923608/148‑1 (MQ=255)
                                                        tATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCt                                                                                           <  1:2133437/148‑1 (MQ=255)
                                                           gCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCtgtg                                                                                        <  2:32075/148‑1 (MQ=255)
                                                            ccAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTTGTGGTCATTGGTAAGTCCCTGACATTTTGTCTGTAACTGAAATGAGCACAATTAATTGGCGGTCCAAAAACCAGCAATTTGATCTGTGc                                                                                       >  2:638846/1‑148 (MQ=255)
                                                                 cAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTGTTTTTTTTTTGTGGGGCTTTGTACGTCCCCGACATTTATTCTGTACCGGAAAGGAGCACAAATAAATTGCGGTGCAAAATCCCCCAGTTTTAGCTGTGCAGAAc                                                                                  >  1:384139/1‑148 (MQ=255)
                                                                   aGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCa                                                                                <  1:1119832/148‑1 (MQ=255)
                                                                      aaCAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTa                                                                             <  1:615374/148‑1 (MQ=255)
                                                                       aCAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTaa                                                                            <  2:3490516/148‑1 (MQ=255)
                                                                          aaCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTa                                                                         <  2:465670/148‑1 (MQ=255)
                                                                           aCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTTGTGGTCAATGGTACGTCCCAGACATTTAGTCTGTACCAGAAACGAGCCCAAATAATTTGCGGTGCAAAAACCAGCATTTTGTGCTGTGCAGAACCCATAAGTat                                                                        >  1:443987/1‑147 (MQ=255)
                                                                           aCTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTTGTACGTCCCTGACATTTAGTATGTAACTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCAATTTTAGGTGTGCAGAACCATTAAGTAg                                                                        >  2:1652001/1‑148 (MQ=255)
                                                                            cTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTTGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCCGAACCCTTAAGTAgc                                                                       >  2:3010583/1‑148 (MQ=255)
                                                                                 tCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTTGTGGTCATTGGTACGTCCCTGACATTTAGTATGTACCTGAAAGGAGCACAATTAATTTGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAAACAATAAGTAGCGCGCt                                                                  >  2:3318646/1‑148 (MQ=255)
                                                                                 tCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTAGTTGGTGGTCATTGGGACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCt                                                                  >  2:1961569/1‑148 (MQ=255)
                                                                                    ttAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTAct                                                               <  2:117996/148‑1 (MQ=255)
                                                                                    ttAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTAca                                                               >  2:1097670/1‑147 (MQ=255)
                                                                                       aCGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTActctc                                                            <  1:1692623/148‑1 (MQ=255)
                                                                                        cGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTActctct                                                           <  2:879671/148‑1 (MQ=255)
                                                                                           cAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttg                                                        <  1:1529493/148‑1 (MQ=255)
                                                                                           cAGTTTGATTCTGGCGTGTTTTTATTTTGTGGTCATTGGTACGTCCCAGACATTTAGTCTGTACCGGAAAGGAGCACAAATAATTTGCGGTCCAAAATCCAGCATTTTGATCTGTGCAGAACCATTAAGTATCGCGCTACACACTttg                                                        >  1:3552892/1‑148 (MQ=255)
                                                                                            aGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgc                                                       <  2:2767067/148‑1 (MQ=255)
                                                                                              tttGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgctg                                                     <  1:2649370/148‑1 (MQ=255)
                                                                                              tttGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgctg                                                     <  2:954347/148‑1 (MQ=255)
                                                                                               ttGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgctgt                                                    <  1:2738008/148‑1 (MQ=255)
                                                                                               ttGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTttgctgt                                                    <  1:2736295/148‑1 (MQ=255)
                                                                                                  aTTCTGGCGTTTTTTTATTTTGTGGTCATTTGTACGTCCCTGACATTTAGTCTGTACCAGAAAGGAGCACAAATAATTTGCGGTCCAAAAGCCAGCAATTTGAGCAGTGCAGAAACATTAATTAGCGCGCAACACTCTtttctgtttc                                                 >  2:3347884/1‑146 (MQ=255)
                                                                                                    tCTGGCGTTTTTTTATTTTGTGGTCATTTGTACGTCCCTGACATTTAGTCTGTAACTGAAAGGAGCACAAATAATTGGCGGTCCAAAAGCCACCATTTTGAGCTGTGCAGAACCATTAAGTATCGCGCTACTCTCTttgctgttgctg                                               >  2:1819933/1‑148 (MQ=255)
                                                                                                      tGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTggg                                             <  1:2878823/148‑1 (MQ=255)
                                                                                                         cGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGAt                                          <  1:242121/148‑1 (MQ=255)
                                                                                                           tttttttATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCa                                        >  1:961879/1‑148 (MQ=255)
                                                                                                             tttttATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACg                                      <  2:1471259/148‑1 (MQ=255)
                                                                                                              ttttATTTGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGt                                     >  2:3422998/1‑148 (MQ=255)
                                                                                                                 tATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTca                                  >  1:892970/1‑148 (MQ=255)
                                                                                                                  aTTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTcac                                 <  2:2655422/148‑1 (MQ=255)
                                                                                                                   tttGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTcaca                                >  2:2491825/1‑148 (MQ=255)
                                                                                                                   tttGGTGGTCATTGGTACGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTcaca                                <  2:540633/148‑1 (MQ=255)
                                                                                                                    ttGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTGGCTGTTGCTGGGGATCACGTTCACAt                               >  1:1101474/1‑148 (MQ=255)
                                                                                                                       gtggtCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTAc                            <  1:1417751/148‑1 (MQ=255)
                                                                                                                       gtggtCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTAc                            <  1:2380641/148‑1 (MQ=255)
                                                                                                                             aTTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGAct                      >  1:668308/1‑148 (MQ=255)
                                                                                                                               tGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActgg                    <  2:1432225/148‑1 (MQ=255)
                                                                                                                               tGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActgg                    <  1:1831581/148‑1 (MQ=255)
                                                                                                                                   aCGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActggctgg                <  1:264044/148‑1 (MQ=255)
                                                                                                                                   aCGTCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGActggctgg                <  1:263448/148‑1 (MQ=255)
                                                                                                                                     gTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGa              <  2:49689/148‑1 (MQ=255)
                                                                                                                                     gTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGa              <  1:2093968/148‑1 (MQ=255)
                                                                                                                                     gTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGa              <  2:531978/148‑1 (MQ=255)
                                                                                                                                      tCCCTGACATTTACTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGaa             >  2:3026199/1‑148 (MQ=255)
                                                                                                                                       cccTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAAc            >  1:1326337/1‑148 (MQ=255)
                                                                                                                                        ccTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACg           >  1:2685099/1‑148 (MQ=255)
                                                                                                                                        ccTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACg           <  2:3021471/148‑1 (MQ=255)
                                                                                                                                         cTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgc          >  2:2216074/1‑148 (MQ=255)
                                                                                                                                          tGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgct         >  1:2702147/1‑148 (MQ=255)
                                                                                                                                           gACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctg        <  2:3542267/148‑1 (MQ=255)
                                                                                                                                            aCATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctgc       >  1:2026854/1‑148 (MQ=255)
                                                                                                                                            aCATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACgctgc       >  1:1565420/1‑148 (MQ=255)
                                                                                                                                               tttAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGa    >  2:956418/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGTTACCAGTCGTCGGCTGGAGTATGCTGATTGGCGGTCTGATTCTGTTGCCTTTTTATGCCAGACAAGGAACAAACTTTGTCGTTAACGGCAGTTTGATTCTGGCGTTTTTTTATTTGGTGGTCATTGGTACGTCCCTGACATTTAGTCTGTACCTGAAAGGAGCACAATTAATTGGCGGTCCAAAAGCCAGCATTTTGAGCTGTGCAGAACCATTAAGTAGCGCGCTACTCTCTTTGCTGTTGCTGGGGATCACGTTCACATTACCGGACTGGCTGGGAACGCTGCTGATT  >  CP009273/3832161‑3832453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A