Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,839,798 C→A 90.3% D244Y (GAT→TAT)  uhpT ← hexose phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,839,7980CA90.3% 585.1 / 59.5 228D244Y (GAT→TAT) uhpThexose phosphate transporter
Reads supporting (aligned to +/- strand):  ref base C (13/9);  new base A (96/110);  total (109/119)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

Only 100 of 228 total aligned reads displayed.

AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAG  >  CP009273/3839653‑3839940
                                                                                                                                                 |                                                                                                                                              
agTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAgg                                                                                                                                              <  1:488171/148‑1 (MQ=255)
  tttCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTa                                                                                                                                            >  1:718499/1‑148 (MQ=255)
  tttCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTa                                                                                                                                            >  1:2497501/1‑148 (MQ=255)
   ttCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAg                                                                                                                                           >  2:526403/1‑148 (MQ=255)
          ccTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGt                                                                                                                                    <  1:543090/148‑1 (MQ=255)
           cTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGtc                                                                                                                                   >  2:2016852/1‑148 (MQ=255)
            tGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGtct                                                                                                                                  <  2:1268222/148‑1 (MQ=255)
             ggAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGtctc                                                                                                                                 <  2:2196369/148‑1 (MQ=255)
              gAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTctct                                                                                                                                <  1:2766253/148‑1 (MQ=255)
               aaCGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCtt                                                                                                                               >  2:2592028/1‑148 (MQ=255)
                   cgTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTc                                                                                                                           <  2:467308/148‑1 (MQ=255)
                   cgTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTc                                                                                                                           <  1:1402778/148‑1 (MQ=255)
                    gTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTcc                                                                                                                          <  2:3216231/148‑1 (MQ=255)
                     tataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCct                                                                                                                         <  2:2181675/148‑1 (MQ=255)
                     tataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCct                                                                                                                         <  2:3255484/148‑1 (MQ=255)
                      ataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctc                                                                                                                        >  1:1016656/1‑148 (MQ=255)
                      ataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctc                                                                                                                        >  1:1516348/1‑148 (MQ=255)
                      ataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctc                                                                                                                        >  2:2260372/1‑148 (MQ=255)
                       taCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctct                                                                                                                       <  1:3113600/148‑1 (MQ=255)
                        aCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCtt                                                                                                                      <  1:3234889/148‑1 (MQ=255)
                         cGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTc                                                                                                                     >  1:1231620/1‑148 (MQ=255)
                           gTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGc                                                                                                                   <  2:1805207/148‑1 (MQ=255)
                               aCCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGAt                                                                                                               <  2:1671741/148‑1 (MQ=255)
                                 cACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGAtct                                                                                                             <  2:1333089/148‑1 (MQ=255)
                                 cACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGAtct                                                                                                             <  2:2121597/148‑1 (MQ=255)
                                   cTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATctcc                                                                                                           <  2:1590724/148‑1 (MQ=255)
                                     ggTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATctcctc                                                                                                         >  1:2819213/1‑148 (MQ=255)
                                           tACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGaa                                                                                                   >  2:3312284/1‑148 (MQ=255)
                                           tACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGaa                                                                                                   <  2:821536/148‑1 (MQ=255)
                                           tACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGaa                                                                                                   >  1:3204203/1‑148 (MQ=255)
                                            aCCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAAc                                                                                                  >  1:1585352/1‑148 (MQ=255)
                                             ccAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACa                                                                                                 >  2:2494593/1‑148 (MQ=255)
                                              cAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAg                                                                                                <  1:178533/148‑1 (MQ=255)
                                              cAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAg                                                                                                >  1:1330664/1‑148 (MQ=255)
                                                aTACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGtt                                                                                              <  2:2508566/148‑1 (MQ=255)
                                                aTACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGtt                                                                                              >  1:1139737/1‑148 (MQ=255)
                                                aTACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGtt                                                                                              >  2:1921106/1‑148 (MQ=255)
                                                aTACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGtt                                                                                              <  2:1795462/148‑1 (MQ=255)
                                                  aCGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGttct                                                                                            <  2:2060328/148‑1 (MQ=255)
                                                  aCGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGttct                                                                                            >  2:1529119/1‑148 (MQ=255)
                                                  aCGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGttct                                                                                            <  2:2032944/148‑1 (MQ=255)
                                                   cGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGttctt                                                                                           <  2:13029/148‑1 (MQ=255)
                                                       ccACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGc                                                                                       <  1:2061157/148‑1 (MQ=255)
                                                         acaTAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCtt                                                                                     >  2:31293/1‑148 (MQ=255)
                                                           aTAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTg                                                                                   <  2:2305600/148‑1 (MQ=255)
                                                            tAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGc                                                                                  <  2:2882920/148‑1 (MQ=255)
                                                               agGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGa                                                                               <  2:461399/148‑1 (MQ=255)
                                                               agGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGa                                                                               >  2:2319815/1‑148 (MQ=255)
                                                               agGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGa                                                                               >  2:3251470/1‑148 (MQ=255)
                                                                    aaTGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCa                                                                          >  1:941469/1‑148 (MQ=255)
                                                                      tGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATa                                                                        <  1:677214/148‑1 (MQ=255)
                                                                      tGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATa                                                                        <  1:1764601/148‑1 (MQ=255)
                                                                        ttGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCTCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAg                                                                      >  1:2732562/1‑148 (MQ=255)
                                                                        ttGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAg                                                                      <  2:223527/148‑1 (MQ=255)
                                                                              aaGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCg                                                                <  2:533946/148‑1 (MQ=255)
                                                                               aGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCgg                                                               <  2:2846865/148‑1 (MQ=255)
                                                                                gCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCggg                                                              >  2:1234796/1‑148 (MQ=255)
                                                                                gCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCggg                                                              <  2:3147618/148‑1 (MQ=255)
                                                                                 cacaGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCgggg                                                             >  1:2097086/1‑148 (MQ=255)
                                                                                 cacaGCAGCCAGATCACTTTGTTTTTCAGAACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCTCAGATCTCCTCTCCGAACAGTTCTACAGCTTTGCCGAGGCCATAAGATACCgggg                                                             >  1:3024631/1‑148 (MQ=255)
                                                                                       agcCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGc                                                       <  1:1037403/148‑1 (MQ=255)
                                                                                         ccAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTg                                                     >  2:1908820/1‑148 (MQ=255)
                                                                                         ccAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTg                                                     >  1:1075932/1‑148 (MQ=255)
                                                                                         ccAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTg                                                     <  1:2136788/148‑1 (MQ=255)
                                                                                         ccAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTg                                                     <  2:2880590/148‑1 (MQ=255)
                                                                                         ccAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTg                                                     <  2:2878430/148‑1 (MQ=255)
                                                                                         ccAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTg                                                     >  1:372125/1‑148 (MQ=255)
                                                                                            gATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCg                                                  <  2:3283118/148‑1 (MQ=255)
                                                                                             aTCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGt                                                 >  2:3245784/1‑148 (MQ=255)
                                                                                              tCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTa                                                >  2:2637967/1‑148 (MQ=255)
                                                                                              tCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTa                                                >  2:2015680/1‑148 (MQ=255)
                                                                                              tCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTa                                                <  1:3235858/148‑1 (MQ=255)
                                                                                               cACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTaa                                               <  2:2279702/148‑1 (MQ=255)
                                                                                               cACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTaa                                               >  1:1794761/1‑148 (MQ=255)
                                                                                                aCTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAAc                                              >  1:1498268/1‑148 (MQ=255)
                                                                                                     gTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAgg                                         >  1:2930252/1‑148 (MQ=255)
                                                                                                     gTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAgg                                         <  1:2420569/148‑1 (MQ=255)
                                                                                                      tttttCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGc                                        <  2:1715265/148‑1 (MQ=255)
                                                                                                         ttCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGa                                     >  2:1587367/1‑148 (MQ=255)
                                                                                                          tCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGAt                                    <  1:901187/148‑1 (MQ=255)
                                                                                                             gCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGGCGTAACGCAGGCCGATaaa                                 >  1:11741/1‑148 (MQ=255)
                                                                                                              cacaTACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATaaaa                                <  1:1298237/148‑1 (MQ=255)
                                                                                                              cacaTACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATaaaa                                >  2:562914/1‑148 (MQ=255)
                                                                                                                 aTACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCg                             >  2:1097699/1‑148 (MQ=255)
                                                                                                                 aTACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCg                             <  1:765410/148‑1 (MQ=255)
                                                                                                                    cTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGGACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACa                          >  1:1951520/1‑148 (MQ=255)
                                                                                                                        acaaAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCa                      <  1:1802152/148‑1 (MQ=255)
                                                                                                                        acaaAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCa                      >  1:2528790/1‑148 (MQ=255)
                                                                                                                         caaAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAg                     >  1:1438789/1‑148 (MQ=255)
                                                                                                                         caaAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAg                     >  2:1500090/1‑148 (MQ=255)
                                                                                                                         caaAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAg                     <  1:338884/148‑1 (MQ=255)
                                                                                                                          aaaGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAgc                    >  1:935460/1‑148 (MQ=255)
                                                                                                                           aaGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAgcg                   <  1:2261570/148‑1 (MQ=255)
                                                                                                                             gATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGcgcg                 >  2:363477/1‑148 (MQ=255)
                                                                                                                               tCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGAt               <  2:850914/148‑1 (MQ=255)
                                                                                                                                 tGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATaa             <  2:1669277/148‑1 (MQ=255)
                                                                                                                                    cACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCg          <  1:44475/148‑1 (MQ=255)
                                                                                                                                      cTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCACGCCGATAAAACCGACAAACAGCGCGATAATCGAc        >  1:122545/1‑148 (MQ=255)
                                                                                                                                        tGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACgg      >  2:2978098/1‑148 (MQ=255)
                                                                                                                                          gTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGa    <  2:1794761/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAG  >  CP009273/3839653‑3839940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATAAAC  >  CP009273/3839649‑3839946
                                                                                                                                                     |                                                                                                                                                    
AGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATA                                                                                                                                                      <  SRR19973070.2361800/150‑1 (MQ=60)
   GAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGT                                                                                                                                                   <  SRR19973070.1287435/150‑1 (MQ=60)
    AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTA                                                                                                                                                  >  SRR19973070.723622/1‑150 (MQ=60)
    AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTA                                                                                                                                                  >  SRR19973070.1475375/1‑150 (MQ=60)
    AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTA                                                                                                                                                  >  SRR19973070.723791/1‑150 (MQ=60)
    AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTA                                                                                                                                                  >  SRR19973070.2513702/1‑150 (MQ=60)
    AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTA                                                                                                                                                  <  SRR19973070.491598/150‑1 (MQ=60)
     GTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAG                                                                                                                                                 >  SRR19973070.13230/1‑150 (MQ=60)
        TCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATT                                                                                                                                              <  SRR19973070.2591972/150‑1 (MQ=60)
         CAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTC                                                                                                                                             <  SRR19973070.1697643/150‑1 (MQ=60)
           GTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGTTAGATTCTG                                                                                                                                           >  SRR19973070.1570160/1‑150 (MQ=60)
           GTTCCTGGAACGCGTATACGGTTGACCACTGGTCGAGACCACTACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTG                                                                                                                                           <  SRR19973070.69254/150‑1 (MQ=60)
              CCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCT                                                                                                                                        <  SRR19973070.89129/150‑1 (MQ=60)
              CCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCT                                                                                                                                        <  SRR19973070.546714/150‑1 (MQ=60)
                TGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCT                                                                                                                                      <  SRR19973070.825926/150‑1 (MQ=60)
                 GGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTT                                                                                                                                     >  SRR19973070.3118060/1‑150 (MQ=60)
                  GAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTT                                                                                                                                    <  SRR19973070.2783971/150‑1 (MQ=60)
                       CGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCT                                                                                                                               >  SRR19973070.1933297/1‑150 (MQ=60)
                       CGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCT                                                                                                                               <  SRR19973070.1412269/150‑1 (MQ=60)
                       CGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCT                                                                                                                               <  SRR19973070.1412250/150‑1 (MQ=60)
                        GTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTC                                                                                                                              >  SRR19973070.1526978/1‑150 (MQ=60)
                        GTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTC                                                                                                                              >  SRR19973070.1023875/1‑150 (MQ=60)
                           TACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTC                                                                                                                           <  SRR19973070.1482863/150‑1 (MQ=60)
                           TACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTC                                                                                                                           <  SRR19973070.3133693/150‑1 (MQ=60)
                           TACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTC                                                                                                                           >  SRR19973070.1240249/1‑150 (MQ=60)
                            ACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCG                                                                                                                          <  SRR19973070.3255768/150‑1 (MQ=60)
                            ACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCG                                                                                                                          <  SRR19973070.3254613/150‑1 (MQ=60)
                                       CTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTC                                                                                                               >  SRR19973070.2837436/1‑150 (MQ=60)
                                        TGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCG                                                                                                              <  SRR19973070.1035706/150‑1 (MQ=60)
                                             GATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAA                                                                                                         >  SRR19973070.3224930/1‑150 (MQ=60)
                                             GATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAA                                                                                                         >  SRR19973070.3224976/1‑150 (MQ=60)
                                              ATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAAC                                                                                                        >  SRR19973070.1596450/1‑150 (MQ=60)
                                              ATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAAC                                                                                                        >  SRR19973070.1781004/1‑150 (MQ=60)
                                                ACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAG                                                                                                      >  SRR19973070.1339841/1‑150 (MQ=60)
                                                ACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAG                                                                                                      <  SRR19973070.1339239/150‑1 (MQ=60)
                                                ACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAG                                                                                                      <  SRR19973070.539980/150‑1 (MQ=60)
                                                ACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAG                                                                                                      >  SRR19973070.2908462/1‑150 (MQ=60)
                                                 CCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGT                                                                                                     <  SRR19973070.454176/150‑1 (MQ=60)
                                                  CAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTT                                                                                                    <  SRR19973070.179949/150‑1 (MQ=60)
                                                  CAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTT                                                                                                    >  SRR19973070.1147640/1‑150 (MQ=60)
                                                   AATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTC                                                                                                   >  SRR19973070.2932142/1‑150 (MQ=60)
                                                           CCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTT                                                                                           <  SRR19973070.2075070/150‑1 (MQ=60)
                                                           CCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTACTCTTCGCTGATCTCCTAGCCGAACAGTTCTTCAGCTT                                                                                           >  SRR19973070.2116065/1‑150 (MQ=60)
                                                             ACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTG                                                                                         <  SRR19973070.1222946/150‑1 (MQ=60)
                                                               ATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCC                                                                                       <  SRR19973070.1284022/150‑1 (MQ=60)
                                                               ATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGACACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCC                                                                                       <  SRR19973070.562983/150‑1 (MQ=60)
                                                                     GAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCC                                                                                 <  SRR19973070.1522696/150‑1 (MQ=60)
                                                                      AAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCA                                                                                >  SRR19973070.947641/1‑150 (MQ=60)
                                                                      AAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCA                                                                                >  SRR19973070.948175/1‑150 (MQ=60)
                                                                        AATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATA                                                                              <  SRR19973070.1090477/150‑1 (MQ=60)
                                                                          TGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCTCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAG                                                                            >  SRR19973070.2750174/1‑150 (MQ=60)
                                                                          TGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAG                                                                            <  SRR19973070.682043/150‑1 (MQ=60)
                                                                          TGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAG                                                                            <  SRR19973070.1776809/150‑1 (MQ=60)
                                                                          TGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAG                                                                            <  SRR19973070.1776812/150‑1 (MQ=60)
                                                                                   AGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGG                                                                   >  SRR19973070.2111174/1‑150 (MQ=60)
                                                                                   AGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGG                                                                   >  SRR19973070.2109856/1‑150 (MQ=60)
                                                                                   AGCACAGCAGCCAGATCACTTTGTTTTTCAGAACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCTCAGATCTCCTCTCCGAACAGTTCTACAGCTTTGCCGAGGCCATAAGATACCGGGG                                                                   >  SRR19973070.3043900/1‑150 (MQ=60)
                                                                                          CAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCT                                                            <  SRR19973070.2235650/150‑1 (MQ=60)
                                                                                           AGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTG                                                           <  SRR19973070.1044734/150‑1 (MQ=60)
                                                                                           AGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTG                                                           >  SRR19973070.1083430/1‑150 (MQ=60)
                                                                                           AGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTG                                                           >  SRR19973070.374855/1‑150 (MQ=60)
                                                                                           AGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTATTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTG                                                           <  SRR19973070.1044765/150‑1 (MQ=60)
                                                                                             CCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCC                                                         <  SRR19973070.2151052/150‑1 (MQ=60)
                                                                                               AGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTCTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGT                                                       >  SRR19973070.392205/1‑150 (MQ=60)
                                                                                                 ATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAA                                                     >  SRR19973070.1807183/1‑150 (MQ=60)
                                                                                                 ATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAA                                                     >  SRR19973070.1807156/1‑150 (MQ=60)
                                                                                                  TCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAAC                                                    >  SRR19973070.1508758/1‑150 (MQ=60)
                                                                                                  TCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAAC                                                    <  SRR19973070.3256653/150‑1 (MQ=60)
                                                                                                  TCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAAC                                                    <  SRR19973070.3256743/150‑1 (MQ=60)
                                                                                                   CACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACG                                                   >  SRR19973070.2766014/1‑150 (MQ=60)
                                                                                                   CACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACG                                                   >  SRR19973070.1956954/1‑150 (MQ=60)
                                                                                                   CACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACG                                                   >  SRR19973070.369965/1‑150 (MQ=60)
                                                                                                   CACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACG                                                   >  SRR19973070.1432892/1‑150 (MQ=60)
                                                                                                    ACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGC                                                  >  SRR19973070.2055612/1‑150 (MQ=60)
                                                                                                     CTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCA                                                 >  SRR19973070.1238140/1‑150 (MQ=60)
                                                                                                      TTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAG                                                <  SRR19973070.356124/150‑1 (MQ=60)
                                                                                                       TTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGG                                               >  SRR19973070.2949157/1‑150 (MQ=60)
                                                                                                         GTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCC                                             <  SRR19973070.2436490/150‑1 (MQ=60)
                                                                                                             TTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATA                                         <  SRR19973070.2516165/150‑1 (MQ=60)
                                                                                                              TCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAA                                        <  SRR19973070.907575/150‑1 (MQ=60)
                                                                                                               CAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGGCGTAACGCAGGCCGATAAA                                       >  SRR19973070.11924/1‑150 (MQ=60)
                                                                                                               CAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAA                                       >  SRR19973070.11734/1‑150 (MQ=60)
                                                                                                                  CACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACC                                    <  SRR19973070.1307275/150‑1 (MQ=60)
                                                                                                                    CATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCGGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGA                                  >  SRR19973070.2470366/1‑150 (MQ=60)
                                                                                                                    CATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGA                                  >  SRR19973070.3156387/1‑150 (MQ=60)
                                                                                                                     ATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGAC                                 <  SRR19973070.770787/150‑1 (MQ=60)
                                                                                                                      TACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGGACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACA                                >  SRR19973070.1964632/1‑150 (MQ=60)
                                                                                                                        CTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAT                              >  SRR19973070.3000812/1‑150 (MQ=60)
                                                                                                                          CAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCA                            >  SRR19973070.2545178/1‑150 (MQ=60)
                                                                                                                          CAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCA                            >  SRR19973070.2545308/1‑150 (MQ=60)
                                                                                                                          CAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCA                            >  SRR19973070.2363054/1‑150 (MQ=60)
                                                                                                                           AACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAG                           >  SRR19973070.2799503/1‑150 (MQ=60)
                                                                                                                           AACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAG                           >  SRR19973070.1448761/1‑150 (MQ=60)
                                                                                                                            ACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGC                          <  SRR19973070.1814597/150‑1 (MQ=60)
                                                                                                                            ACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGC                          >  SRR19973070.942130/1‑150 (MQ=60)
                                                                                                                            ACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGC                          >  SRR19973070.942106/1‑150 (MQ=60)
                                                                                                                             CAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCG                         >  SRR19973070.3280825/1‑150 (MQ=60)
                                                                                                                             CAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCG                         <  SRR19973070.341355/150‑1 (MQ=60)
                                                                                                                               AAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCG                       <  SRR19973070.2276575/150‑1 (MQ=60)
                                                                                                                               AAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCG                       <  SRR19973070.2276306/150‑1 (MQ=60)
                                                                                                                                      GCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCG                >  SRR19973070.608978/1‑150 (MQ=60)
                                                                                                                                        CACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGAC              <  SRR19973070.45042/150‑1 (MQ=60)
                                                                                                                                        CACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCACGCCGATAAAACCGACAAACAGCGCGATAATCGAC              >  SRR19973070.123635/1‑150 (MQ=60)
                                                                                                                                              GTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAG        >  SRR19973070.180389/1‑150 (MQ=60)
                                                                                                                                                AATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGAT      <  SRR19973070.1148427/150‑1 (MQ=60)
                                                                                                                                                   ATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAAAAGCGCGATAATAGACGGGAAGATAAA   >  SRR19973070.294130/1‑150 (MQ=60)
                                                                                                                                                    TAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATAAAC  >  SRR19973070.1383812/1‑150 (MQ=60)
                                                                                                                                                    TAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATAAAC  >  SRR19973070.1383591/1‑150 (MQ=60)
                                                                                                                                                    TAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATAAAC  >  SRR19973070.1383789/1‑150 (MQ=60)
                                                                                                                                                    TAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGGAACGCAGGCCGATAAAAACGACAATCAGCGCGAGACTCGACCGGAAGATAAAC  >  SRR19973070.1532975/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATAAAC  >  CP009273/3839649‑3839946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑