Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F218 I0 R1	1506	278.7	9275112	94.5%	8764980	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	3,841,409	+CAACCAG	14.9%	coding (576/1320 nt)	uhpC ←	membrane protein regulates uhpT expression

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 3841409	200 (0.820)	36 (0.140) +CAACCAG	9/244	NT	14.9%	coding (576/1320 nt)	uhpC	membrane protein regulates uhpT expression
*	?	CP009273	3841410 =	217 (0.830)	36 (0.140) +CAACCAG	9/244	NT	14.9%	coding (575/1320 nt)	uhpC	membrane protein regulates uhpT expression

GGATCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/3841261‑3841409

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGT  >  CP009273/3841410‑3841555

                                                                                                                                                     |||||||

GGATCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATAC                                                                                                                                                          <  1:4339563/150‑1

 GATCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACA                                                                                                                                                         >  1:3659644/1‑150

 GATCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACA                                                                                                                                                         <  1:340419/150‑1

   TCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAAC                                                                                                                                                       >  2:189162/1‑150

   TCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAAC                                                                                                                                                       >  1:4122402/1‑150

   TCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAAC                                                                                                                                                       >  1:2427945/1‑150

   TCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAAC                                                                                                                                                       >  2:189120/1‑150

    CTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACC                                                                                                                                                      >  1:1949505/1‑150

     TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     >  2:2432073/1‑150

     TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     <  1:4308185/150‑1

     TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     >  1:4058989/1‑150

     TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     >  1:3426033/1‑150

     TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     >  1:3426040/1‑150

     TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     >  1:3426044/1‑150

     TCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCA                                                                                                                                                     >  1:3426066/1‑150

      CTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAG                                                                                                                                                    <  1:4507230/150‑1

      CTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAG                                                                                                                                                    >  2:119755/1‑150

      CTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAG                                                                                                                                                    >  2:1943066/1‑150

      CTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAG                                                                                                                                                    >  2:1943088/1‑150

      CTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAG                                                                                                                                                    >  2:1943148/1‑150

       TTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGC                                                                                                                                                   <  1:4376857/150‑1

        TTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCA                                                                                                                                                  >  1:704459/1‑150

         TACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAA                                                                                                                                                 >  1:330692/1‑150

         TACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAA                                                                                                                                                 >  2:4143463/1‑150

         TACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAA                                                                                                                                                 <  2:966100/150‑1

         TACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTAGCGCCATACAACCAGCca                                                                                                                                                 >  1:3596011/1‑148

                              CTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCA                                                                                                                            <  1:2565370/150‑1

                              CTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCA                                                                                                                            <  2:549219/150‑1

                                           AATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAAT                                                                                                               <  2:2214531/150‑1

                                                                                  TAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGC                                                                        >  1:1287306/1‑150

                                                                                                      GATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTG                                                    >  1:4250531/1‑150

                                                                                                             TAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATA                                             >  1:1760034/1‑150

                                                                                                                          AGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACC                                >  2:2895720/1‑150

                                                                                                                          AGAAAAATCCCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACC                                >  2:9471/1‑150

                                                                                                                                   CCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACACTGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTC                       <  1:2264849/150‑1

                                                                                                                                   CCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTC                       <  1:2530648/150‑1

                                                                                                                                   CCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTC                       <  1:2264853/150‑1

                                                                                                                                   CCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTC                       <  2:4060439/150‑1

                                                                                                                                   CCCACGACTATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTC                       <  1:2264850/150‑1

                                                                                                                                            ATCGCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGA              >  2:2952557/1‑150

                                                                                                                                               GCCATACAACCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATA           >  1:2822244/1‑150

                                                                                                                                                        CCAGCAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGT  <  1:305350/150‑1

                                                                                                                                                     |||||||

GGATCTCTTTACGCGTCAACCCTGCCCCTTCTTGTTGTTGAGCAATTTCCAGCGCGTCGTGTCGCCATTCACCGACCGCCGGTAAACCTAACGCCTGCGGGCGATCGCGTAGCCGCCAGCAGAGAAAAATCCCCACGACTATCGCCATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/3841261‑3841409

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAACCAGCAATCATCATCCCGGCACGCCAGCCGTAATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGT  >  CP009273/3841410‑3841555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A