Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,839,798 C→A 100% D244Y (GAT→TAT)  uhpT ← hexose phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,839,7980CA96.2% 713.9 / 17.2 239D244Y (GAT→TAT) uhpThexose phosphate transporter
Reads supporting (aligned to +/- strand):  ref base C (4/5);  new base A (132/98);  total (136/103)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 241 total aligned reads displayed.

AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGAT  >  CP009273/3839653‑3839942
                                                                                                                                                 |                                                                                                                                                
agTTTGAGTTCCTTGAACGCGTATACGTTTGACCACTTGTCGATACCAATACGTACCCCATAGAGGATATTGTTGGCGAAGCACAGCAGCCATATCACTTTGTTTTTCAGCACCTACTCTACAAAGTTCTGCCACTTGGTCATATAgg                                                                                                                                                <  1:677328/148‑1 (MQ=255)
  tttCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATCTAGGTa                                                                                                                                              >  1:2108169/1‑148 (MQ=255)
  tttCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTa                                                                                                                                              >  1:414076/1‑148 (MQ=255)
   ttCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAg                                                                                                                                             >  2:1165151/1‑148 (MQ=255)
   ttCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAg                                                                                                                                             <  1:2667641/148‑1 (MQ=255)
    tCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGa                                                                                                                                            <  2:1249436/148‑1 (MQ=255)
    tCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGa                                                                                                                                            <  2:1250322/148‑1 (MQ=255)
     cAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGAt                                                                                                                                           >  2:372233/1‑148 (MQ=255)
          ccTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGt                                                                                                                                      <  1:1419768/148‑1 (MQ=255)
            ttgAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGtct                                                                                                                                    >  1:2809409/3‑148 (MQ=255)
            tGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGtct                                                                                                                                    >  1:962666/1‑148 (MQ=255)
              gAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTctct                                                                                                                                  <  1:1238654/148‑1 (MQ=255)
              gAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTctct                                                                                                                                  >  2:2402719/1‑148 (MQ=255)
               aaCGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCtt                                                                                                                                 <  1:3197242/148‑1 (MQ=255)
               aaCGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCtt                                                                                                                                 <  2:152090/148‑1 (MQ=255)
                   cgTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTc                                                                                                                             >  2:735224/1‑148 (MQ=255)
                      ataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctc                                                                                                                          >  1:1147310/1‑148 (MQ=255)
                       taCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctct                                                                                                                         <  2:3066029/148‑1 (MQ=255)
                       taCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctct                                                                                                                         <  1:1267375/148‑1 (MQ=255)
                        aCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCtt                                                                                                                        >  1:2755176/1‑148 (MQ=255)
                          ggTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCg                                                                                                                      <  2:945978/148‑1 (MQ=255)
                          ggTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTGTGTCCTCTTCg                                                                                                                      >  2:2720245/1‑148 (MQ=255)
                               aCCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGAt                                                                                                                 <  1:2196137/148‑1 (MQ=255)
                               aCCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACGTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGAt                                                                                                                 >  2:3423429/1‑148 (MQ=255)
                                ccACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGAtc                                                                                                                <  2:2847617/148‑1 (MQ=255)
                                  aCTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGAtctc                                                                                                              <  2:2217213/148‑1 (MQ=255)
                                   cTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATctcc                                                                                                             <  1:295777/148‑1 (MQ=255)
                                   cTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATctcc                                                                                                             >  2:2298119/1‑148 (MQ=255)
                                   cTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATctcc                                                                                                             >  2:2470385/1‑148 (MQ=255)
                                      gTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCg                                                                                                          >  2:78129/1‑148 (MQ=255)
                                       tCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGc                                                                                                         >  2:1886155/1‑148 (MQ=255)
                                             ccAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACa                                                                                                   <  1:1318209/148‑1 (MQ=255)
                                              cAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAg                                                                                                  >  2:2534720/1‑148 (MQ=255)
                                              cAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAg                                                                                                  <  2:68503/148‑1 (MQ=255)
                                               aaTACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGt                                                                                                 >  1:2216635/1‑148 (MQ=255)
                                                aTACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGtt                                                                                                <  2:1949046/148‑1 (MQ=255)
                                                aTACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGtt                                                                                                <  1:1965713/148‑1 (MQ=255)
                                                  aCGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGttct                                                                                              >  2:1809200/1‑148 (MQ=255)
                                                   cGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGttctt                                                                                             <  1:1109906/148‑1 (MQ=255)
                                                   cGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGttctt                                                                                             >  1:2223396/1‑148 (MQ=255)
                                                      aCCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAg                                                                                          >  1:125365/1‑148 (MQ=255)
                                                      aCCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAg                                                                                          >  1:835816/1‑148 (MQ=255)
                                                         acaTAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCtt                                                                                       >  1:1035334/1‑148 (MQ=255)
                                                             agagGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGcc                                                                                   >  2:1274102/1‑148 (MQ=255)
                                                              gagGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCg                                                                                  >  1:2183333/1‑148 (MQ=255)
                                                               agGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGa                                                                                 >  2:1406468/1‑148 (MQ=255)
                                                               agGAAAAAGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTATTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGACTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACACTTCTTCAGCTATGCCGa                                                                                 >  2:1314745/1‑148 (MQ=255)
                                                                   aaaTGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGcc                                                                             >  2:2166245/1‑148 (MQ=255)
                                                                    aaTGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCa                                                                            <  1:2023405/148‑1 (MQ=255)
                                                                    aaTGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCa                                                                            >  2:561577/1‑148 (MQ=255)
                                                                      tGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATa                                                                          >  1:1850420/1‑148 (MQ=255)
                                                                        ttGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAg                                                                        >  2:2764205/1‑148 (MQ=255)
                                                                        ttGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAg                                                                        >  2:2764560/1‑148 (MQ=255)
                                                                             gAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTcc                                                                   <  1:1624653/148‑1 (MQ=255)
                                                                              aaGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCg                                                                  <  1:1797432/148‑1 (MQ=255)
                                                                              aaGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCg                                                                  <  1:3123639/148‑1 (MQ=255)
                                                                                gCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCggg                                                                >  1:2143423/1‑148 (MQ=255)
                                                                                 cacaGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATACTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCgggg                                                               >  1:54359/1‑148 (MQ=255)
                                                                                    agcagcCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGt                                                            >  2:3264043/1‑148 (MQ=255)
                                                                                      cagcCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCg                                                          <  2:2084117/148‑1 (MQ=255)
                                                                                      cagcCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCg                                                          <  1:538541/148‑1 (MQ=255)
                                                                                      cagcCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCg                                                          >  1:2449902/1‑148 (MQ=255)
                                                                                      cagcCAGATCACTTTGTTTTTCAGCACATAATCAACAAAGATCTGCCACTTGGTCCTATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTGTGCCGAGGCCATAAGATTCCGCGGAGTCg                                                          >  1:2449821/1‑148 (MQ=255)
                                                                                        gcCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCt                                                        >  1:139312/1‑148 (MQ=255)
                                                                                              tCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTa                                                  >  1:215814/1‑148 (MQ=255)
                                                                                              tCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTa                                                  >  2:2133830/1‑148 (MQ=255)
                                                                                              tCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTa                                                  >  2:1448145/1‑148 (MQ=255)
                                                                                               cACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTaa                                                 >  2:3205651/1‑148 (MQ=255)
                                                                                                   ttGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCa                                             <  1:1424893/148‑1 (MQ=255)
                                                                                                   ttGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCa                                             >  2:2211737/1‑148 (MQ=255)
                                                                                                   ttGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCa                                             >  1:2533265/1‑148 (MQ=255)
                                                                                                      tttttCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGc                                          <  2:2655683/148‑1 (MQ=255)
                                                                                                      tttttCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGc                                          >  2:2354176/1‑148 (MQ=255)
                                                                                                        tttCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCg                                        <  1:2379784/148‑1 (MQ=255)
                                                                                                        tttCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCg                                        <  2:826594/148‑1 (MQ=255)
                                                                                                         ttCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGa                                       >  2:3352969/1‑148 (MQ=255)
                                                                                                               acaTACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAAc                                 <  2:1027878/148‑1 (MQ=255)
                                                                                                                 aTACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCg                               <  2:645787/148‑1 (MQ=255)
                                                                                                                   aCTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGAc                             >  1:1673710/1‑148 (MQ=255)
                                                                                                                    cTCACCAAAGATCTGCCAGTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATACGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACa                            <  2:3379925/148‑1 (MQ=255)
                                                                                                                    cTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACa                            >  2:519523/1‑148 (MQ=255)
                                                                                                                      caacaaAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGTGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAt                          >  1:838071/1‑148 (MQ=255)
                                                                                                                      caacaaAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGTGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAt                          >  1:482920/1‑148 (MQ=255)
                                                                                                                      caacaaAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAt                          <  2:3206273/148‑1 (MQ=255)
                                                                                                                         caaAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAg                       >  1:3536986/1‑148 (MQ=255)
                                                                                                                         caaAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAg                       <  1:1433143/148‑1 (MQ=255)
                                                                                                                          aaaGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAgc                      >  2:246051/1‑148 (MQ=255)
                                                                                                                          aaaGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAgc                      >  1:1829325/1‑148 (MQ=255)
                                                                                                                           aaGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAgcg                     <  1:2640510/148‑1 (MQ=255)
                                                                                                                              aTCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGa                  >  1:2254950/1‑148 (MQ=255)
                                                                                                                              aTCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGa                  >  1:255291/1‑148 (MQ=255)
                                                                                                                              aTCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACCCAGGCCGATAAAACCGACAATCAGCGCGa                  >  1:904723/1‑148 (MQ=255)
                                                                                                                               tCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGAt                 <  2:2942626/148‑1 (MQ=255)
                                                                                                                                 tGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATaa               <  1:156310/148‑1 (MQ=255)
                                                                                                                                    cACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCg            <  1:2286158/148‑1 (MQ=255)
                                                                                                                                     aCTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAATATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGa           <  2:962666/148‑1 (MQ=255)
                                                                                                                                       ttGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACg         >  2:1464652/1‑148 (MQ=255)
                                                                                                                                           tCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGaa     >  1:1488496/1‑148 (MQ=255)
                                                                                                                                             atatAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGa   <  2:1131259/148‑1 (MQ=255)
                                                                                                                                              tataGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGAt  >  2:1903504/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGAT  >  CP009273/3839653‑3839942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATAAACA  >  CP009273/3839649‑3839947
                                                                                                                                                     |                                                                                                                                                     
AGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATA                                                                                                                                                       >  SRR19973067.2198354/1‑150 (MQ=60)
  AGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGG                                                                                                                                                     <  SRR19973067.1023026/150‑1 (MQ=60)
    AGTTTGAGTTCCTTGAACGCGTATACGTTTGACCACTTGTCGATACCAATACGTACCCCATAGAGGATATTGTTGGCGAAGCACAGCAGCCATATCACTTTGTTTTTCAGCACCTACTCTACAAAGTTCTGCCACTTGGTCATATAGGTA                                                                                                                                                   <  SRR19973067.681975/150‑1 (MQ=60)
    AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATCTAGGTA                                                                                                                                                   >  SRR19973067.2122171/1‑150 (MQ=60)
    AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTA                                                                                                                                                   >  SRR19973067.416932/1‑150 (MQ=60)
    AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTA                                                                                                                                                   >  SRR19973067.416953/1‑150 (MQ=60)
       TTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGAT                                                                                                                                                <  SRR19973067.2684777/150‑1 (MQ=60)
              CCTTGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCT                                                                                                                                         >  SRR19973067.2827518/1‑150 (MQ=60)
              CCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCT                                                                                                                                         <  SRR19973067.50346/150‑1 (MQ=60)
              CCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCT                                                                                                                                         >  SRR19973067.969261/1‑150 (MQ=60)
              CCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCT                                                                                                                                         <  SRR19973067.1437445/150‑1 (MQ=60)
              CCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCT                                                                                                                                         <  SRR19973067.1437440/150‑1 (MQ=60)
              CCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCT                                                                                                                                         <  SRR19973067.1429402/150‑1 (MQ=60)
              CCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCT                                                                                                                                         <  SRR19973067.1429363/150‑1 (MQ=60)
                  GAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTT                                                                                                                                     <  SRR19973067.1901514/150‑1 (MQ=60)
                  GAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTT                                                                                                                                     <  SRR19973067.90005/150‑1 (MQ=60)
                  GAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTT                                                                                                                                     <  SRR19973067.1246973/150‑1 (MQ=60)
                   AACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTG                                                                                                                                    <  SRR19973067.3217414/150‑1 (MQ=60)
                        GTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTC                                                                                                                               >  SRR19973067.2312043/1‑150 (MQ=60)
                        GTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTC                                                                                                                               >  SRR19973067.1155000/1‑150 (MQ=60)
                        GTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCAAAGCAGCCATATCACTTTGTTTTTCATCACATACTCAACAAATATCTGCCACTTGGTCATATATGTAGATTCTGTATCTTTGTCCTC                                                                                                                               >  SRR19973067.1154980/1‑150 (MQ=60)
                          ATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTT                                                                                                                             >  SRR19973067.2772980/1‑150 (MQ=60)
                           TACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTC                                                                                                                            <  SRR19973067.1275983/150‑1 (MQ=60)
                           TACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTC                                                                                                                            <  SRR19973067.1275986/150‑1 (MQ=60)
                           TACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCCGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTC                                                                                                                            <  SRR19973067.1275976/150‑1 (MQ=60)
                            ACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCG                                                                                                                           <  SRR19973067.952760/150‑1 (MQ=60)
                              GGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCT                                                                                                                         <  SRR19973067.2062605/150‑1 (MQ=60)
                                  GACCACTGGTCGATACCAAGACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATC                                                                                                                     <  SRR19973067.2255410/150‑1 (MQ=60)
                                   ACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCT                                                                                                                    <  SRR19973067.2210787/150‑1 (MQ=60)
                                       CTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTC                                                                                                                <  SRR19973067.297668/150‑1 (MQ=60)
                                           TCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCG                                                                                                            >  SRR19973067.2178695/1‑150 (MQ=60)
                                           TCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCG                                                                                                            >  SRR19973067.1961416/1‑150 (MQ=60)
                                            CGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGA                                                                                                           <  SRR19973067.1242114/150‑1 (MQ=60)
                                             GATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAA                                                                                                          >  SRR19973067.418025/1‑150 (MQ=60)
                                              ATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAAC                                                                                                         >  SRR19973067.507104/1‑150 (MQ=60)
                                                 CCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGT                                                                                                      >  SRR19973067.2231364/1‑150 (MQ=60)
                                                 CCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGT                                                                                                      >  SRR19973067.2231398/1‑150 (MQ=60)
                                                 CCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGT                                                                                                      <  SRR19973067.1327208/150‑1 (MQ=60)
                                                    ATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCT                                                                                                   <  SRR19973067.1979057/150‑1 (MQ=60)
                                                    ATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCT                                                                                                   <  SRR19973067.2486044/150‑1 (MQ=60)
                                                    ATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCT                                                                                                   <  SRR19973067.310719/150‑1 (MQ=60)
                                                     TACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTT                                                                                                  >  SRR19973067.2238199/1‑150 (MQ=60)
                                                     TACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTT                                                                                                  >  SRR19973067.2237811/1‑150 (MQ=60)
                                                     TACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTT                                                                                                  <  SRR19973067.2155538/150‑1 (MQ=60)
                                                       CGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCA                                                                                                <  SRR19973067.1117434/150‑1 (MQ=60)
                                                        GTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAG                                                                                               >  SRR19973067.841438/1‑150 (MQ=60)
                                                        GTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAG                                                                                               >  SRR19973067.126525/1‑150 (MQ=60)
                                                        GTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAG                                                                                               >  SRR19973067.126534/1‑150 (MQ=60)
                                                        GTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAG                                                                                               >  SRR19973067.841497/1‑150 (MQ=60)
                                                        GTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAG                                                                                               >  SRR19973067.841505/1‑150 (MQ=60)
                                                           CCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTT                                                                                            >  SRR19973067.1042457/1‑150 (MQ=60)
                                                           CCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTT                                                                                            >  SRR19973067.1538178/1‑150 (MQ=60)
                                                                TAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCG                                                                                       >  SRR19973067.2197930/1‑150 (MQ=60)
                                                                TAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCG                                                                                       >  SRR19973067.2198549/1‑150 (MQ=60)
                                                                       AAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCAT                                                                                <  SRR19973067.546637/150‑1 (MQ=60)
                                                                        AATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATA                                                                               >  SRR19973067.1863034/1‑150 (MQ=60)
                                                                        AATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATA                                                                               <  SRR19973067.227891/150‑1 (MQ=60)
                                                                        AATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATA                                                                               <  SRR19973067.2036986/150‑1 (MQ=60)
                                                                          TGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAG                                                                             <  SRR19973067.3172239/150‑1 (MQ=60)
                                                                              GGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTC                                                                         >  SRR19973067.1380644/1‑150 (MQ=60)
                                                                              GGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTC                                                                         <  SRR19973067.1412435/150‑1 (MQ=60)
                                                                                CGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCG                                                                       >  SRR19973067.1686152/1‑150 (MQ=60)
                                                                                 GAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGG                                                                      <  SRR19973067.1635798/150‑1 (MQ=60)
                                                                                  AAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGG                                                                     <  SRR19973067.1809517/150‑1 (MQ=60)
                                                                                  AAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGG                                                                     <  SRR19973067.3143495/150‑1 (MQ=60)
                                                                                  AAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGG                                                                     <  SRR19973067.1809516/150‑1 (MQ=60)
                                                                                  AAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGG                                                                     >  SRR19973067.2157756/1‑150 (MQ=60)
                                                                                  AAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGG                                                                     <  SRR19973067.1808627/150‑1 (MQ=60)
                                                                                  AAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGG                                                                     >  SRR19973067.2157748/1‑150 (MQ=60)
                                                                                   AGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATACTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGG                                                                    >  SRR19973067.55052/1‑150 (MQ=60)
                                                                                        AGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCG                                                               >  SRR19973067.2466000/1‑150 (MQ=60)
                                                                                        AGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCG                                                               >  SRR19973067.2465963/1‑150 (MQ=60)
                                                                                        AGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCG                                                               >  SRR19973067.2465420/1‑150 (MQ=60)
                                                                                        AGCAGCCAGATCACTTTGTTTTTCAGCACATAATCAACAAAGATCTGCCACTTGGTCCTATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTGTGCCGAGGCCATAAGATTCCGCGGAGTCG                                                               >  SRR19973067.2465917/1‑150 (MQ=60)
                                                                                          CAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCT                                                             <  SRR19973067.542134/150‑1 (MQ=60)
                                                                                          CAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCT                                                             >  SRR19973067.140547/1‑150 (MQ=60)
                                                                                              CAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTTCAACATGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGATGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGACATAAGATTCAGGGGAGTCGCTGCAG                                                         >  SRR19973067.2005130/1‑150 (MQ=60)
                                                                                              CAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCG                                                         >  SRR19973067.2794104/1‑150 (MQ=60)
                                                                                                GATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTA                                                       >  SRR19973067.1086827/1‑150 (MQ=60)
                                                                                                GATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTA                                                       >  SRR19973067.217383/1‑150 (MQ=60)
                                                                                                GATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTA                                                       >  SRR19973067.217409/1‑150 (MQ=60)
                                                                                                  TCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAAC                                                     >  SRR19973067.2258813/1‑150 (MQ=60)
                                                                                                  TCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAAC                                                     <  SRR19973067.1416001/150‑1 (MQ=60)
                                                                                                   CACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACG                                                    >  SRR19973067.373/1‑150 (MQ=60)
                                                                                                     CTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCA                                                  >  SRR19973067.756015/1‑150 (MQ=60)
                                                                                                     CTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCA                                                  >  SRR19973067.2549863/1‑150 (MQ=60)
                                                                                                       TTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGG                                                <  SRR19973067.1434512/150‑1 (MQ=60)
                                                                                                       TTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGG                                                <  SRR19973067.267376/150‑1 (MQ=60)
                                                                                                       TTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGG                                                <  SRR19973067.267453/150‑1 (MQ=60)
                                                                                                       TTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGG                                                >  SRR19973067.2635743/1‑150 (MQ=60)
                                                                                                            TTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGAT                                           <  SRR19973067.2395241/150‑1 (MQ=60)
                                                                                                            TTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGAT                                           <  SRR19973067.2493082/150‑1 (MQ=60)
                                                                                                            TTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGAT                                           <  SRR19973067.1616544/150‑1 (MQ=60)
                                                                                                               CAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAA                                        >  SRR19973067.161139/1‑150 (MQ=60)
                                                                                                               CAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAA                                        >  SRR19973067.1484276/1‑150 (MQ=60)
                                                                                                                     ATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGAC                                  >  SRR19973067.3216363/1‑150 (MQ=60)
                                                                                                                     ATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGAC                                  >  SRR19973067.2730718/1‑150 (MQ=60)
                                                                                                                     ATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGAC                                  >  SRR19973067.1685251/1‑150 (MQ=60)
                                                                                                                       ACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAA                                >  SRR19973067.2911228/1‑150 (MQ=60)
                                                                                                                        CTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGTGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAT                               >  SRR19973067.843762/1‑150 (MQ=60)
                                                                                                                        CTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGTGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAT                               >  SRR19973067.843781/1‑150 (MQ=60)
                                                                                                                        CTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGTGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAT                               >  SRR19973067.486242/1‑150 (MQ=60)
                                                                                                                           AACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAG                            >  SRR19973067.2459398/1‑150 (MQ=60)
                                                                                                                           AACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAG                            <  SRR19973067.1247596/150‑1 (MQ=60)
                                                                                                                           AACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAG                            <  SRR19973067.2221882/150‑1 (MQ=60)
                                                                                                                           AACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAG                            >  SRR19973067.3559335/1‑150 (MQ=60)
                                                                                                                            ACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGC                           >  SRR19973067.1841758/1‑150 (MQ=60)
                                                                                                                            ACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGC                           >  SRR19973067.1841906/1‑150 (MQ=60)
                                                                                                                             CAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCG                          <  SRR19973067.1442797/150‑1 (MQ=60)
                                                                                                                               AAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCG                        <  SRR19973067.2657573/150‑1 (MQ=60)
                                                                                                                               AAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCG                        <  SRR19973067.1649746/150‑1 (MQ=60)
                                                                                                                                AGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGA                       >  SRR19973067.2269919/1‑150 (MQ=60)
                                                                                                                                AGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGA                       >  SRR19973067.257037/1‑150 (MQ=60)
                                                                                                                                AGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACCCAGGCCGATAAAACCGACAATCAGCGCGA                       >  SRR19973067.910696/1‑150 (MQ=60)
                                                                                                                                 GATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGAT                      <  SRR19973067.2811469/150‑1 (MQ=60)
                                                                                                                                   TCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAA                    <  SRR19973067.666761/150‑1 (MQ=60)
                                                                                                                                    CTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAAT                   <  SRR19973067.3367694/150‑1 (MQ=60)
                                                                                                                                     TGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATC                  <  SRR19973067.157621/150‑1 (MQ=60)
                                                                                                                                     TGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATC                  <  SRR19973067.2551327/150‑1 (MQ=60)
                                                                                                                                      GCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCG                 <  SRR19973067.206002/150‑1 (MQ=60)
                                                                                                                                        CACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGAC               <  SRR19973067.2301270/150‑1 (MQ=60)
                                                                                                                                        CACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGAC               <  SRR19973067.1172895/150‑1 (MQ=60)
                                                                                                                                             GGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAA          >  SRR19973067.1498352/1‑150 (MQ=60)
                                                                                                                                                     AGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATAAACA  >  SRR19973067.961723/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATAAACA  >  CP009273/3839649‑3839947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑