Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,839,798 C→A 100% D244Y (GAT→TAT)  uhpT ← hexose phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,839,7980CA99.5% 656.7 / ‑5.7 207D244Y (GAT→TAT) uhpThexose phosphate transporter
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (90/113);  total (93/114)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 207 total aligned reads displayed.

AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGA  >  CP009273/3839653‑3839941
                                                                                                                                                 |                                                                                                                                               
agTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAgg                                                                                                                                               <  1:1181253/148‑1 (MQ=255)
agTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAgg                                                                                                                                               >  2:2415446/1‑148 (MQ=255)
  tttCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTa                                                                                                                                             >  1:1236554/1‑148 (MQ=255)
   ttCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAg                                                                                                                                            <  1:955982/148‑1 (MQ=255)
   ttCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAg                                                                                                                                            <  1:3395831/148‑1 (MQ=255)
   ttCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAg                                                                                                                                            <  2:412352/148‑1 (MQ=255)
    tCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGa                                                                                                                                           <  1:431186/148‑1 (MQ=255)
      aGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGAtt                                                                                                                                         >  1:2686040/1‑148 (MQ=255)
       gTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTc                                                                                                                                        <  2:1044756/148‑1 (MQ=255)
       gTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTc                                                                                                                                        >  2:1301107/1‑148 (MQ=255)
        ttCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACACAGATCTGCCACGTGGTCCTATCGGTAGATTCt                                                                                                                                       >  2:2987202/1‑148 (MQ=255)
        ttCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCt                                                                                                                                       >  2:1609318/1‑148 (MQ=255)
          ccTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGt                                                                                                                                     >  1:1026784/1‑148 (MQ=255)
          ccTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGt                                                                                                                                     <  2:2977232/148‑1 (MQ=255)
            tGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCTTATAGGTAGATTCTGtct                                                                                                                                   <  2:1159480/148‑1 (MQ=255)
              gAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTctct                                                                                                                                 <  1:1884064/148‑1 (MQ=255)
              gAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTctct                                                                                                                                 <  1:1479938/148‑1 (MQ=255)
               aaCGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCtt                                                                                                                                <  1:3145400/148‑1 (MQ=255)
                 cgcgTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTg                                                                                                                              <  2:1696802/148‑1 (MQ=255)
                      ataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctc                                                                                                                         <  1:2253232/148‑1 (MQ=255)
                      ataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctc                                                                                                                         <  2:650590/148‑1 (MQ=255)
                      ataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctc                                                                                                                         >  1:379907/1‑148 (MQ=255)
                      ataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctc                                                                                                                         >  1:2638657/1‑148 (MQ=255)
                      ataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctc                                                                                                                         >  2:1840368/1‑148 (MQ=255)
                      ataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctc                                                                                                                         >  2:1840450/1‑148 (MQ=255)
                      ataCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctc                                                                                                                         <  2:2261896/148‑1 (MQ=255)
                       taCGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCctct                                                                                                                        <  2:2056597/148‑1 (MQ=255)
                          ggTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCg                                                                                                                     <  1:1829991/148‑1 (MQ=255)
                          ggTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCg                                                                                                                     <  1:950525/148‑1 (MQ=255)
                          ggTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCg                                                                                                                     <  1:653898/148‑1 (MQ=255)
                          ggTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCg                                                                                                                     <  1:2665627/148‑1 (MQ=255)
                             tGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTg                                                                                                                  <  2:559280/148‑1 (MQ=255)
                              gACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGa                                                                                                                 <  2:2567768/148‑1 (MQ=255)
                                ccACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGAtc                                                                                                               <  1:739666/148‑1 (MQ=255)
                                 cACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGAtct                                                                                                              >  2:2915661/1‑148 (MQ=255)
                                    tGGTCGATACCACTACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATctcct                                                                                                           <  2:461984/148‑1 (MQ=255)
                                       tCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCCTATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGc                                                                                                        >  2:3019445/1‑148 (MQ=255)
                                       tCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGc                                                                                                        <  1:1389475/148‑1 (MQ=255)
                                        cGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGcc                                                                                                       >  2:597338/1‑148 (MQ=255)
                                           tACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGaa                                                                                                    >  2:732411/1‑148 (MQ=255)
                                           tACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGaa                                                                                                    >  1:2904005/1‑148 (MQ=255)
                                           tACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGaa                                                                                                    >  2:771568/1‑148 (MQ=255)
                                            aCCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAAc                                                                                                   >  1:60530/1‑148 (MQ=255)
                                             ccAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACa                                                                                                  >  1:172650/1‑148 (MQ=255)
                                             ccAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACa                                                                                                  <  2:2672839/148‑1 (MQ=255)
                                              cAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAg                                                                                                 >  2:2842093/1‑148 (MQ=255)
                                               aaTACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGt                                                                                                >  2:250651/1‑148 (MQ=255)
                                                   cGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGttctt                                                                                            <  1:1682125/148‑1 (MQ=255)
                                                      aCCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAg                                                                                         >  2:984632/1‑148 (MQ=255)
                                                       ccACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGc                                                                                        <  1:1060145/148‑1 (MQ=255)
                                                        cacaTAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCt                                                                                       <  1:2195544/148‑1 (MQ=255)
                                                         acaTAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCtt                                                                                      <  1:1330291/148‑1 (MQ=255)
                                                            tAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGc                                                                                   >  2:1084060/1‑148 (MQ=255)
                                                            tAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGc                                                                                   >  1:2966976/1‑148 (MQ=255)
                                                               agGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGa                                                                                <  1:2679577/148‑1 (MQ=255)
                                                                 gAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAgg                                                                              >  2:1570301/1‑148 (MQ=255)
                                                                  aaaaTGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGc                                                                             <  1:203839/148‑1 (MQ=255)
                                                                    aaTGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCa                                                                           <  2:2442732/148‑1 (MQ=255)
                                                                      tGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATa                                                                         <  1:1301107/148‑1 (MQ=255)
                                                                          ggCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGAt                                                                     <  2:1026784/148‑1 (MQ=255)
                                                                          ggCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGAt                                                                     <  1:2946305/148‑1 (MQ=255)
                                                                              aaGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCg                                                                 <  1:1119535/148‑1 (MQ=255)
                                                                                 cacaGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCgggg                                                              >  2:1443719/1‑148 (MQ=255)
                                                                                 cacaGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCgggg                                                              <  2:1261705/148‑1 (MQ=255)
                                                                                 cacaGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCgggg                                                              >  2:429115/1‑148 (MQ=255)
                                                                                  acaGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGa                                                             <  2:844855/148‑1 (MQ=255)
                                                                                    agcagcCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGACATATAGCTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTATTCCGAGGCCATAAGATTCCGGGGAGt                                                           >  1:2086641/1‑148 (MQ=255)
                                                                                      cagcCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTCGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCg                                                         >  2:2613545/1‑148 (MQ=255)
                                                                                      cagcCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCg                                                         <  2:379907/148‑1 (MQ=255)
                                                                                         ccAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTg                                                      <  1:3220449/148‑1 (MQ=255)
                                                                                         ccAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTg                                                      >  1:3362167/1‑148 (MQ=255)
                                                                                          cAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGc                                                     >  1:1270238/1‑148 (MQ=255)
                                                                                             aTCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGt                                                  >  1:2399444/1‑148 (MQ=255)
                                                                                             aTCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGt                                                  >  2:618244/1‑148 (MQ=255)
                                                                                              tCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTa                                                 >  1:2518493/1‑148 (MQ=255)
                                                                                              tCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTa                                                 >  2:1907873/1‑148 (MQ=255)
                                                                                                  tttGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGc                                             >  2:115802/1‑148 (MQ=255)
                                                                                                   ttGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCGTAAGATTCCGGGGAGTCGCTGCCGTAACGCa                                            <  2:3112070/148‑1 (MQ=255)
                                                                                                   ttGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCa                                            <  2:3112007/148‑1 (MQ=255)
                                                                                                          tCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGAt                                     <  2:722006/148‑1 (MQ=255)
                                                                                                             gCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATaaa                                  <  1:3083787/148‑1 (MQ=255)
                                                                                                               acaTACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAAc                                <  1:771568/148‑1 (MQ=255)
                                                                                                                  tACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGa                             >  2:3435604/1‑148 (MQ=255)
                                                                                                                   aCTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGAc                            >  1:957395/1‑148 (MQ=255)
                                                                                                                     tCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACaa                          <  1:1015569/148‑1 (MQ=255)
                                                                                                                     tCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACaa                          <  1:3005863/148‑1 (MQ=255)
                                                                                                                     tCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACaa                          <  1:3070906/148‑1 (MQ=255)
                                                                                                                     tCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGAAAAAACAGACaa                          >  2:310109/1‑148 (MQ=255)
                                                                                                                      caacaaAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAt                         >  1:2309145/1‑148 (MQ=255)
                                                                                                                      caacaaAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAt                         <  2:203045/148‑1 (MQ=255)
                                                                                                                        acaaAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCa                       >  2:2646125/1‑148 (MQ=255)
                                                                                                                             gATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGcgcg                  <  2:2903950/148‑1 (MQ=255)
                                                                                                                             gATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGcgcg                  <  2:2021423/148‑1 (MQ=255)
                                                                                                                                   ccACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATc            >  1:724252/1‑148 (MQ=255)
                                                                                                                                     aCTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGa          <  1:1609318/148‑1 (MQ=255)
                                                                                                                                     aCTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGa          >  1:1353043/1‑148 (MQ=255)
                                                                                                                                     aCTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGa          >  1:687668/1‑148 (MQ=255)
                                                                                                                                          gTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGTCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGa     <  2:133152/148‑1 (MQ=255)
                                                                                                                                            cATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAg   <  2:2660816/148‑1 (MQ=255)
                                                                                                                                            cATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAg   <  1:3400547/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGA  >  CP009273/3839653‑3839941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATAAAC  >  CP009273/3839649‑3839946
                                                                                                                                                     |                                                                                                                                                    
AGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATA                                                                                                                                                      >  SRR19973066.1652267/1‑150 (MQ=60)
   GAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGT                                                                                                                                                   <  SRR19973066.1206982/150‑1 (MQ=60)
   GAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGT                                                                                                                                                   <  SRR19973066.1206755/150‑1 (MQ=60)
    AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTA                                                                                                                                                  >  SRR19973066.1245354/1‑150 (MQ=60)
    AGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTA                                                                                                                                                  <  SRR19973066.1189555/150‑1 (MQ=60)
       TTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGAT                                                                                                                                               <  SRR19973066.3418483/150‑1 (MQ=60)
       TTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGAT                                                                                                                                               <  SRR19973066.1771278/150‑1 (MQ=60)
       TTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGAT                                                                                                                                               <  SRR19973066.3418965/150‑1 (MQ=60)
       TTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGAT                                                                                                                                               <  SRR19973066.962973/150‑1 (MQ=60)
       TTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGAT                                                                                                                                               <  SRR19973066.962974/150‑1 (MQ=60)
        TCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATT                                                                                                                                              <  SRR19973066.434524/150‑1 (MQ=60)
        TCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATT                                                                                                                                              >  SRR19973066.2703727/1‑150 (MQ=60)
        TCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATT                                                                                                                                              >  SRR19973066.2703956/1‑150 (MQ=60)
         CAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTC                                                                                                                                             <  SRR19973066.972240/150‑1 (MQ=60)
          AGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCT                                                                                                                                            >  SRR19973066.3006970/1‑150 (MQ=60)
            TTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGT                                                                                                                                          >  SRR19973066.1034340/1‑150 (MQ=60)
            TTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGT                                                                                                                                          <  SRR19973066.1423140/150‑1 (MQ=60)
             TCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTC                                                                                                                                         >  SRR19973066.134088/1‑150 (MQ=60)
             TCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTC                                                                                                                                         >  SRR19973066.134453/1‑150 (MQ=60)
                  GAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTT                                                                                                                                    <  SRR19973066.1296620/150‑1 (MQ=60)
                  GAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTT                                                                                                                                    <  SRR19973066.1897504/150‑1 (MQ=60)
                  GAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTT                                                                                                                                    <  SRR19973066.1490340/150‑1 (MQ=60)
                  GAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTT                                                                                                                                    <  SRR19973066.1490364/150‑1 (MQ=60)
                   AACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTG                                                                                                                                   <  SRR19973066.3166381/150‑1 (MQ=60)
                        GTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTC                                                                                                                              >  SRR19973066.2656414/1‑150 (MQ=60)
                        GTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTC                                                                                                                              <  SRR19973066.689022/150‑1 (MQ=60)
                        GTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTC                                                                                                                              <  SRR19973066.688971/150‑1 (MQ=60)
                        GTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTC                                                                                                                              >  SRR19973066.2656424/1‑150 (MQ=60)
                        GTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTC                                                                                                                              >  SRR19973066.382897/1‑150 (MQ=60)
                          ATACGGTTGACCACTGGTCGATACCAATACGTCCCACATAGAGGAAAATGTTGGCGAAGCACCGCAGCCCGATCACTTTGTTTTTCAGCACATACTCAACCAAGATCTGCCACTTGGGCATATAGGTAGATTCTGTCTCTTTGTCCTCTT                                                                                                                            >  SRR19973066.1270811/1‑150 (MQ=60)
                          ATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTT                                                                                                                            <  SRR19973066.2268765/150‑1 (MQ=60)
                          ATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTT                                                                                                                            >  SRR19973066.3095377/1‑150 (MQ=60)
                           TACGGTTGACCACTGGTCGATACCAATACGTACCACCTAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTC                                                                                                                           >  SRR19973066.1648182/1‑150 (MQ=60)
                              GGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCT                                                                                                                        <  SRR19973066.658668/150‑1 (MQ=60)
                              GGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCT                                                                                                                        <  SRR19973066.1843057/150‑1 (MQ=60)
                              GGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCT                                                                                                                        <  SRR19973066.1843253/150‑1 (MQ=60)
                              GGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCT                                                                                                                        <  SRR19973066.957473/150‑1 (MQ=60)
                              GGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCT                                                                                                                        <  SRR19973066.2683804/150‑1 (MQ=60)
                              GGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCT                                                                                                                        <  SRR19973066.2683470/150‑1 (MQ=60)
                              GGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCT                                                                                                                        <  SRR19973066.2491878/150‑1 (MQ=60)
                              GGTTGACCACTGGTCGATAACAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCT                                                                                                                        <  SRR19973066.957653/150‑1 (MQ=60)
                                    CCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTC                                                                                                                  <  SRR19973066.745619/150‑1 (MQ=60)
                                    CCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTC                                                                                                                  <  SRR19973066.745158/150‑1 (MQ=60)
                                           TCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCG                                                                                                           <  SRR19973066.1399506/150‑1 (MQ=60)
                                             GATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAA                                                                                                         >  SRR19973066.2923349/1‑150 (MQ=60)
                                             GATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAA                                                                                                         >  SRR19973066.2923404/1‑150 (MQ=60)
                                              ATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAAC                                                                                                        >  SRR19973066.61280/1‑150 (MQ=60)
                                               TACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACA                                                                                                       >  SRR19973066.174186/1‑150 (MQ=60)
                                               TACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACA                                                                                                       >  SRR19973066.3405388/1‑150 (MQ=60)
                                                ACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAG                                                                                                      <  SRR19973066.635569/150‑1 (MQ=60)
                                                   AATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTC                                                                                                   <  SRR19973066.1143612/150‑1 (MQ=60)
                                                   AATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTC                                                                                                   <  SRR19973066.2932748/150‑1 (MQ=60)
                                                       CGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCA                                                                                               <  SRR19973066.1694471/150‑1 (MQ=60)
                                                       CGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCA                                                                                               <  SRR19973066.1694374/150‑1 (MQ=60)
                                                          ACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCT                                                                                            <  SRR19973066.704410/150‑1 (MQ=60)
                                                           CCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTT                                                                                           <  SRR19973066.1067927/150‑1 (MQ=60)
                                                           CCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTT                                                                                           <  SRR19973066.1067871/150‑1 (MQ=60)
                                                            CACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTT                                                                                          <  SRR19973066.2210905/150‑1 (MQ=60)
                                                            CACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTT                                                                                          <  SRR19973066.2210771/150‑1 (MQ=60)
                                                             ACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTG                                                                                         <  SRR19973066.3126274/150‑1 (MQ=60)
                                                             ACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTG                                                                                         <  SRR19973066.1339939/150‑1 (MQ=60)
                                                              CATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGC                                                                                        >  SRR19973066.2986601/1‑150 (MQ=60)
                                                                   AGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGG                                                                                   <  SRR19973066.2697462/150‑1 (MQ=60)
                                                                      AAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCA                                                                                <  SRR19973066.205558/150‑1 (MQ=60)
                                                                          TGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAG                                                                            <  SRR19973066.1310537/150‑1 (MQ=60)
                                                                              GGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTC                                                                        <  SRR19973066.2965840/150‑1 (MQ=60)
                                                                              GGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTC                                                                        <  SRR19973066.2965863/150‑1 (MQ=60)
                                                                              GGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTC                                                                        <  SRR19973066.2965872/150‑1 (MQ=60)
                                                                               GCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCC                                                                       <  SRR19973066.393687/150‑1 (MQ=60)
                                                                                 GAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGG                                                                     <  SRR19973066.80908/150‑1 (MQ=60)
                                                                                  AAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGG                                                                    <  SRR19973066.1127601/150‑1 (MQ=60)
                                                                                   AGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGG                                                                   >  SRR19973066.529442/1‑150 (MQ=60)
                                                                                     CACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAG                                                                 >  SRR19973066.3045806/1‑150 (MQ=60)
                                                                                      ACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGACATATAGCTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTATTCCGAGGCCATAAGATTCCGGGGAGT                                                                >  SRR19973066.2101033/1‑150 (MQ=60)
                                                                                           AGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTG                                                           >  SRR19973066.3384584/1‑150 (MQ=60)
                                                                                            GCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGC                                                          >  SRR19973066.1279412/1‑150 (MQ=60)
                                                                                             CCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCC                                                         <  SRR19973066.3241758/150‑1 (MQ=60)
                                                                                               AGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGT                                                       >  SRR19973066.2415632/1‑150 (MQ=60)
                                                                                               AGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTACAGCTTTGCCGAGCCCATAAGATTCCGGGGAGTCGCTGCCGG                                                       >  SRR19973066.2415624/1‑150 (MQ=60)
                                                                                                GATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTA                                                      >  SRR19973066.2535622/1‑150 (MQ=60)
                                                                                                  TCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAAC                                                    >  SRR19973066.2100248/1‑150 (MQ=60)
                                                                                                                 GCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAAC                                     <  SRR19973066.3104390/150‑1 (MQ=60)
                                                                                                                 GCACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAAC                                     >  SRR19973066.1205038/1‑150 (MQ=60)
                                                                                                                   ACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCG                                   <  SRR19973066.777281/150‑1 (MQ=60)
                                                                                                                   ACATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCG                                   <  SRR19973066.737845/150‑1 (MQ=60)
                                                                                                                     ATACTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGAC                                 >  SRR19973066.964400/1‑150 (MQ=60)
                                                                                                                        CTCAACAAAGATCTGCCAGTTGGTCATATCGTTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAT                              <  SRR19973066.3166470/150‑1 (MQ=60)
                                                                                                                        CTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAT                              >  SRR19973066.2324777/1‑150 (MQ=60)
                                                                                                                        CTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAT                              >  SRR19973066.1932873/1‑150 (MQ=60)
                                                                                                                        CTCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAAT                              >  SRR19973066.2324954/1‑150 (MQ=60)
                                                                                                                         TCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATC                             <  SRR19973066.3091436/150‑1 (MQ=60)
                                                                                                                         TCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATC                             <  SRR19973066.3025854/150‑1 (MQ=60)
                                                                                                                         TCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATC                             <  SRR19973066.1023045/150‑1 (MQ=60)
                                                                                                                         TCAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATC                             <  SRR19973066.1106418/150‑1 (MQ=60)
                                                                                                                          CAACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAAACGACAATCA                            >  SRR19973066.22269/1‑150 (MQ=60)
                                                                                                                           AACAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAG                           >  SRR19973066.1029031/1‑150 (MQ=60)
                                                                                                                             CAAAGATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCG                         >  SRR19973066.3358314/1‑150 (MQ=60)
                                                                                                                                  ATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATA                    <  SRR19973066.3446591/150‑1 (MQ=60)
                                                                                                                                  ATCTGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATA                    <  SRR19973066.1828607/150‑1 (MQ=60)
                                                                                                                                     TGCCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATC                 >  SRR19973066.729619/1‑150 (MQ=60)
                                                                                                                                       CCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGA               >  SRR19973066.692721/1‑150 (MQ=60)
                                                                                                                                       CCACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGA               >  SRR19973066.1362843/1‑150 (MQ=60)
                                                                                                                                        CACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGAC              <  SRR19973066.1372469/150‑1 (MQ=60)
                                                                                                                                         ACTTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACG             <  SRR19973066.1620993/150‑1 (MQ=60)
                                                                                                                                           TTGGTCATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGG           <  SRR19973066.2619004/150‑1 (MQ=60)
                                                                                                                                                CATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGAT      <  SRR19973066.3423249/150‑1 (MQ=60)
                                                                                                                                                 ATATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATA     >  SRR19973066.3396449/1‑150 (MQ=60)
                                                                                                                                                   ATAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATAAA   >  SRR19973066.121535/1‑150 (MQ=60)
                                                                                                                                                    TAGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATAAAC  <  SRR19973066.1409366/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAGGCCGATAAAACCGACAATCAGCGCGATAATCGACGGGAAGATAAAC  >  CP009273/3839649‑3839946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑