Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,245,537 G→T 100% N286K (AAC→AAA)  plsB ← glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,245,5370GT96.0% 555.9 / 12.3 188N286K (AAC→AAA) plsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (2/6);  new base T (80/99);  total (83/105)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 189 total aligned reads displayed.

GGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAT  >  CP009273/4245392‑4245680
                                                                                                                                                 |                                                                                                                                               
ggTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCttt                                                                                                                                               <  1:2091381/148‑1 (MQ=255)
 gTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTa                                                                                                                                              <  1:3052703/148‑1 (MQ=255)
  ttGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTAt                                                                                                                                             <  1:616567/148‑1 (MQ=255)
  ttGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTAt                                                                                                                                             <  1:732898/148‑1 (MQ=255)
  ttGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTAt                                                                                                                                             <  1:1012010/148‑1 (MQ=255)
   tGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATg                                                                                                                                            >  2:3007876/1‑148 (MQ=255)
   tGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATg                                                                                                                                            >  2:1107225/1‑148 (MQ=255)
    gATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATgg                                                                                                                                           <  2:1213423/148‑1 (MQ=255)
       cccGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACg                                                                                                                                        >  2:1458253/1‑148 (MQ=255)
        ccGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGATCGCGCTCAGCTTTATGGACGt                                                                                                                                       <  1:2827332/148‑1 (MQ=255)
         cggcggCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGtt                                                                                                                                      <  2:1032695/148‑1 (MQ=255)
          ggcggcGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTg                                                                                                                                     <  1:122095/148‑1 (MQ=255)
           gcggcgATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGa                                                                                                                                    >  2:1507044/1‑148 (MQ=255)
               cgATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGcc                                                                                                                                <  1:601759/148‑1 (MQ=255)
                gATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGccc                                                                                                                               <  1:1127503/148‑1 (MQ=255)
                gATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGccc                                                                                                                               <  1:78402/148‑1 (MQ=255)
                    tGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGt                                                                                                                           <  2:1827642/148‑1 (MQ=255)
                    tGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGt                                                                                                                           <  2:168250/148‑1 (MQ=255)
                              ccAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGtt                                                                                                                 <  1:2121770/148‑1 (MQ=255)
                              ccAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGtt                                                                                                                 >  1:3156653/1‑148 (MQ=255)
                              ccAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGtt                                                                                                                 >  1:2751009/1‑148 (MQ=255)
                              ccAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGtt                                                                                                                 >  1:1515125/1‑148 (MQ=255)
                               cAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTc                                                                                                                >  1:220370/1‑148 (MQ=255)
                               cAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTc                                                                                                                <  1:2972291/148‑1 (MQ=255)
                               cAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTc                                                                                                                <  1:2887046/148‑1 (MQ=255)
                                   cccTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAgg                                                                                                            <  1:2349216/148‑1 (MQ=255)
                                     cTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTg                                                                                                          >  2:2686187/1‑148 (MQ=255)
                                      tGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGa                                                                                                         <  2:172998/148‑1 (MQ=255)
                                      tGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGa                                                                                                         >  1:1763679/1‑148 (MQ=255)
                                      tGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGa                                                                                                         >  2:762650/1‑148 (MQ=255)
                                       ggTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGaa                                                                                                        >  2:3193179/1‑148 (MQ=255)
                                           aTACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGccc                                                                                                    >  2:21364/1‑148 (MQ=255)
                                             aCAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAg                                                                                                  >  2:503178/1‑148 (MQ=255)
                                                 cACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATa                                                                                              >  1:1627453/1‑148 (MQ=255)
                                                     tAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgt                                                                                          >  2:2916803/1‑148 (MQ=255)
                                                     tAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgt                                                                                          >  2:3159486/1‑148 (MQ=255)
                                                         aaaGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagt                                                                                      >  2:207941/1‑148 (MQ=255)
                                                          aaGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagtc                                                                                     >  2:2340459/1‑148 (MQ=255)
                                                          aaGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagtc                                                                                     >  2:2340481/1‑148 (MQ=255)
                                                           agcagcAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagtca                                                                                    >  1:3058221/1‑148 (MQ=255)
                                                           agcagcAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagtca                                                                                    >  1:789554/1‑148 (MQ=255)
                                                           agcagcAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagtca                                                                                    >  2:2913488/1‑148 (MQ=255)
                                                            gcagcaGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGtcagtcag                                                                                   >  1:2596729/1‑148 (MQ=255)
                                                            gcagcaGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGtcagtcag                                                                                   >  2:2505652/1‑148 (MQ=255)
                                                                cagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGa                                                                               >  1:1263180/1‑148 (MQ=255)
                                                                cagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGa                                                                               <  1:2922048/148‑1 (MQ=255)
                                                                   gTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatc                                                                            >  1:1227907/1‑148 (MQ=255)
                                                                     aGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatcat                                                                          <  1:528325/148‑1 (MQ=255)
                                                                     aGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatcat                                                                          <  1:528339/148‑1 (MQ=255)
                                                                      gTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatcatc                                                                         >  1:792462/1‑148 (MQ=255)
                                                                       tCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTCAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCAtct                                                                        >  2:2021561/1‑148 (MQ=255)
                                                                       tCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCAtct                                                                        <  2:1786314/148‑1 (MQ=255)
                                                                        ccATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCAtctc                                                                       <  2:1809972/148‑1 (MQ=255)
                                                                        ccATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCAtctc                                                                       <  2:3060045/148‑1 (MQ=255)
                                                                            gtgACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTaa                                                                   >  1:3193196/1‑148 (MQ=255)
                                                                            gtgACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTaa                                                                   >  1:2883127/1‑148 (MQ=255)
                                                                            gtgACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTaa                                                                   >  1:1160704/1‑148 (MQ=255)
                                                                                 tGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGaa                                                              <  2:1075148/148‑1 (MQ=255)
                                                                                 tGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGaa                                                              <  1:1063042/148‑1 (MQ=255)
                                                                                  gCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGaaa                                                             <  1:1170134/148‑1 (MQ=255)
                                                                                   cGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAAt                                                            <  2:1820660/148‑1 (MQ=255)
                                                                                      tGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCTAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATTCGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTct                                                         <  2:616335/148‑2 (MQ=255)
                                                                                      tGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcg                                                         <  2:2278664/148‑1 (MQ=255)
                                                                                       ggCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgc                                                        >  1:769206/1‑148 (MQ=255)
                                                                                         cAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgccg                                                      >  1:3150202/1‑148 (MQ=255)
                                                                                             gCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt                                                  <  2:881486/148‑1 (MQ=255)
                                                                                              cacaTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAtc                                                 <  1:2913436/148‑1 (MQ=255)
                                                                                              cacaTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAtc                                                 <  1:2913668/148‑1 (MQ=255)
                                                                                               acaTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAtct                                                <  1:2835512/148‑1 (MQ=255)
                                                                                                 atatACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATctct                                              >  1:3224062/1‑148 (MQ=255)
                                                                                                 atatACCAGCTCATGACAGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATctct                                              >  1:1739712/1‑148 (MQ=255)
                                                                                                   ataCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTc                                            <  2:353313/148‑1 (MQ=255)
                                                                                                     aCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCGTCCa                                          <  1:2848794/148‑1 (MQ=255)
                                                                                                      ccAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAt                                         >  1:2909504/1‑148 (MQ=255)
                                                                                                       cAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATc                                        >  2:1457689/1‑148 (MQ=255)
                                                                                                        aGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCa                                       >  1:891330/1‑148 (MQ=255)
                                                                                                              tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGTTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCaa                                 <  1:1107225/148‑1 (MQ=255)
                                                                                                              tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCaa                                 <  1:8339/148‑1 (MQ=255)
                                                                                                              tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCaa                                 <  1:1685549/148‑1 (MQ=255)
                                                                                                              tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCaa                                 <  2:3242776/148‑1 (MQ=255)
                                                                                                                  cgtcgtGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgc                             <  2:2564230/148‑1 (MQ=255)
                                                                                                                       tGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTTGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTct                        >  1:2133542/1‑148 (MQ=255)
                                                                                                                       tGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTTGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTct                        >  1:2135401/1‑148 (MQ=255)
                                                                                                                        gggCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctg                       <  2:170322/148‑1 (MQ=255)
                                                                                                                         ggCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctgc                      <  1:3007876/148‑1 (MQ=255)
                                                                                                                          gCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctgct                     >  2:1291434/1‑148 (MQ=255)
                                                                                                                           ccAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctgctg                    <  2:2901815/148‑1 (MQ=255)
                                                                                                                           ccAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctgctg                    <  2:3088890/148‑1 (MQ=255)
                                                                                                                             aGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcg                  >  2:25976/1‑148 (MQ=255)
                                                                                                                              gCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcgc                 <  1:902434/148‑1 (MQ=255)
                                                                                                                                 ggCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttt              <  2:2766471/148‑1 (MQ=255)
                                                                                                                                  gCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCtttt             <  1:1458253/148‑1 (MQ=255)
                                                                                                                                    gAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTc           <  1:2653224/148‑1 (MQ=255)
                                                                                                                                    gAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTc           <  1:2931060/148‑1 (MQ=255)
                                                                                                                                         cgcTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATggg      <  1:2517975/148‑1 (MQ=255)
                                                                                                                                         cgcTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATggg      <  1:1473354/148‑1 (MQ=255)
                                                                                                                                           cTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGgag    <  2:1644628/148‑1 (MQ=255)
                                                                                                                                           cTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGgag    <  2:1417047/148‑1 (MQ=255)
                                                                                                                                            tCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGgaga   <  2:1687924/148‑1 (MQ=255)
                                                                                                                                             cAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAt  <  1:433050/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAT  >  CP009273/4245392‑4245680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTT  >  CP009273/4245390‑4245685
                                                                                                                                                   |                                                                                                                                                    
CAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTT                                                                                                                                                    <  SRR19973070.2983030/150‑1 (MQ=60)
  GGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTAT                                                                                                                                                  <  SRR19973070.2105442/150‑1 (MQ=60)
   GTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATG                                                                                                                                                 <  SRR19973070.3072165/150‑1 (MQ=60)
    TTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGG                                                                                                                                                <  SRR19973070.738087/150‑1 (MQ=60)
    TTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGG                                                                                                                                                <  SRR19973070.620859/150‑1 (MQ=60)
    TTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGG                                                                                                                                                <  SRR19973070.1019203/150‑1 (MQ=60)
    TTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGG                                                                                                                                                <  SRR19973070.620889/150‑1 (MQ=60)
     TGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGA                                                                                                                                               >  SRR19973070.3239051/1‑150 (MQ=60)
       ATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACG                                                                                                                                             <  SRR19973070.1767501/150‑1 (MQ=60)
          CCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGATCGCGCTCAGCTTTATGGACGTTG                                                                                                                                          <  SRR19973070.2845626/150‑1 (MQ=60)
          CCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTG                                                                                                                                          <  SRR19973070.2845668/150‑1 (MQ=60)
            GGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGAT                                                                                                                                        <  SRR19973070.122829/150‑1 (MQ=60)
            GGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGAT                                                                                                                                        <  SRR19973070.123183/150‑1 (MQ=60)
            GGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGAT                                                                                                                                        <  SRR19973070.123185/150‑1 (MQ=60)
                 CGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCT                                                                                                                                   <  SRR19973070.605951/150‑1 (MQ=60)
                  GATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTG                                                                                                                                  <  SRR19973070.482367/150‑1 (MQ=60)
                  GATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTG                                                                                                                                  <  SRR19973070.1135267/150‑1 (MQ=60)
                  GATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTG                                                                                                                                  <  SRR19973070.79227/150‑1 (MQ=60)
                  GATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTG                                                                                                                                  <  SRR19973070.79184/150‑1 (MQ=60)
                          GCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTC                                                                                                                          <  SRR19973070.807153/150‑1 (MQ=60)
                          GCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTC                                                                                                                          <  SRR19973070.757445/150‑1 (MQ=60)
                          GCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTC                                                                                                                          <  SRR19973070.3041254/150‑1 (MQ=60)
                              CACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTT                                                                                                                      >  SRR19973070.2768681/1‑150 (MQ=60)
                              CACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTT                                                                                                                      >  SRR19973070.3177057/1‑150 (MQ=60)
                              CACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTT                                                                                                                      >  SRR19973070.1525740/1‑150 (MQ=60)
                               ACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTC                                                                                                                     >  SRR19973070.221968/1‑150 (MQ=60)
                                CCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCC                                                                                                                    <  SRR19973070.2135346/150‑1 (MQ=60)
                                CCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCC                                                                                                                    <  SRR19973070.2135975/150‑1 (MQ=60)
                                 CAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCA                                                                                                                   <  SRR19973070.2991382/150‑1 (MQ=60)
                                 CAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCA                                                                                                                   <  SRR19973070.2991358/150‑1 (MQ=60)
                                 CAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCA                                                                                                                   <  SRR19973070.1247536/150‑1 (MQ=60)
                                 CAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCA                                                                                                                   <  SRR19973070.2905737/150‑1 (MQ=60)
                                 CAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCA                                                                                                                   <  SRR19973070.2905786/150‑1 (MQ=60)
                                     CCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTG                                                                                                               <  SRR19973070.2364794/150‑1 (MQ=60)
                                      CCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGA                                                                                                              >  SRR19973070.1775883/1‑150 (MQ=60)
                                        TGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAG                                                                                                            <  SRR19973070.540920/150‑1 (MQ=60)
                                          GTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCC                                                                                                          <  SRR19973070.1559579/150‑1 (MQ=60)
                                                CAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAAT                                                                                                    >  SRR19973070.1082498/1‑150 (MQ=60)
                                                 AGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATA                                                                                                   >  SRR19973070.1638749/1‑150 (MQ=60)
                                                        AAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAG                                                                                            <  SRR19973070.2290708/150‑1 (MQ=60)
                                                           AAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCA                                                                                         <  SRR19973070.3169412/150‑1 (MQ=60)
                                                           AAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCA                                                                                         >  SRR19973070.3077773/1‑150 (MQ=60)
                                                           AAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCA                                                                                         >  SRR19973070.795029/1‑150 (MQ=60)
                                                            AAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAG                                                                                        >  SRR19973070.2461366/1‑150 (MQ=60)
                                                            AAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAG                                                                                        >  SRR19973070.2613694/1‑150 (MQ=60)
                                                                AGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGA                                                                                    >  SRR19973070.1272028/1‑150 (MQ=60)
                                                                  CAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAAT                                                                                  <  SRR19973070.2940914/150‑1 (MQ=60)
                                                                  CAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAAT                                                                                  <  SRR19973070.2940919/150‑1 (MQ=60)
                                                                   AGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATC                                                                                 <  SRR19973070.2709243/150‑1 (MQ=60)
                                                                   AGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATC                                                                                 >  SRR19973070.1236503/1‑150 (MQ=60)
                                                                   AGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATC                                                                                 <  SRR19973070.2206702/150‑1 (MQ=60)
                                                                    GGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCTTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGGTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACCGTCAGTCAGGCGAATCA                                                                                >  SRR19973070.721484/1‑150 (MQ=60)
                                                                    GGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCA                                                                                >  SRR19973070.1289239/1‑150 (MQ=60)
                                                                     GTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCAT                                                                               <  SRR19973070.906882/150‑1 (MQ=60)
                                                                      TAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATTCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATC                                                                              >  SRR19973070.797679/1‑150 (MQ=60)
                                                                      TAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATC                                                                              >  SRR19973070.797946/1‑150 (MQ=60)
                                                                       AGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCT                                                                             <  SRR19973070.531894/150‑1 (MQ=60)
                                                                       AGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCT                                                                             <  SRR19973070.531908/150‑1 (MQ=60)
                                                                            ATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAA                                                                        >  SRR19973070.1168839/1‑150 (MQ=60)
                                                                            ATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAA                                                                        >  SRR19973070.2902349/1‑150 (MQ=60)
                                                                            ATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAA                                                                        >  SRR19973070.2901792/1‑150 (MQ=60)
                                                                            ATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAA                                                                        >  SRR19973070.2901781/1‑150 (MQ=60)
                                                                            ATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAA                                                                        >  SRR19973070.1168824/1‑150 (MQ=60)
                                                                            ATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAA                                                                        >  SRR19973070.3213862/1‑150 (MQ=60)
                                                                                   TGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAAT                                                                 <  SRR19973070.1070498/150‑1 (MQ=60)
                                                                                    GCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATT                                                                <  SRR19973070.1178325/150‑1 (MQ=60)
                                                                                       GTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGC                                                             >  SRR19973070.774602/1‑150 (MQ=60)
                                                                                         GGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCG                                                           >  SRR19973070.3170651/1‑150 (MQ=60)
                                                                                         GGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCG                                                           >  SRR19973070.3170560/1‑150 (MQ=60)
                                                                                            AAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTAAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAA                                                        >  SRR19973070.2640824/1‑150 (MQ=60)
                                                                                                CACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTC                                                    <  SRR19973070.2932315/150‑1 (MQ=60)
                                                                                                CACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTC                                                    >  SRR19973070.3073457/1‑150 (MQ=60)
                                                                                                CACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTC                                                    <  SRR19973070.2932496/150‑1 (MQ=60)
                                                                                                CACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTC                                                    <  SRR19973070.2932263/150‑1 (MQ=60)
                                                                                                 ACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCT                                                   <  SRR19973070.2853863/150‑1 (MQ=60)
                                                                                                 ACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCT                                                   >  SRR19973070.3244896/1‑150 (MQ=60)
                                                                                                 ACATATACCAGCTCATGACAGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCT                                                   >  SRR19973070.1751774/1‑150 (MQ=60)
                                                                                                  CATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTT                                                  >  SRR19973070.2587643/1‑150 (MQ=60)
                                                                                                  CATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTT                                                  <  SRR19973070.2141266/150‑1 (MQ=60)
                                                                                                   ATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTC                                                 >  SRR19973070.1126065/1‑150 (MQ=60)
                                                                                                   ATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCAACTCGTAAGAGAAATTCGCCGCAATCTCTTC                                                 >  SRR19973070.335080/1‑150 (MQ=60)
                                                                                                      TACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAT                                              >  SRR19973070.2928317/1‑150 (MQ=60)
                                                                                                      TACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAT                                              >  SRR19973070.2928993/1‑150 (MQ=60)
                                                                                                       ACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCGTCCATC                                             <  SRR19973070.2867243/150‑1 (MQ=60)
                                                                                                        CCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCA                                            >  SRR19973070.897626/1‑150 (MQ=60)
                                                                                                               ATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAAT                                     <  SRR19973070.1517589/150‑1 (MQ=60)
                                                                                                                TGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGTTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATC                                    <  SRR19973070.1114809/150‑1 (MQ=60)
                                                                                                                TGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATC                                    <  SRR19973070.8463/150‑1 (MQ=60)
                                                                                                                TGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATC                                    <  SRR19973070.1697088/150‑1 (MQ=60)
                                                                                                                TGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATC                                    <  SRR19973070.1697111/150‑1 (MQ=60)
                                                                                                                       CGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTTGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCT                             >  SRR19973070.2149662/1‑150 (MQ=60)
                                                                                                                       CGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTTGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCT                             >  SRR19973070.2147800/1‑150 (MQ=60)
                                                                                                                       CGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCT                             <  SRR19973070.794676/150‑1 (MQ=60)
                                                                                                                          GGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCT                          <  SRR19973070.170040/150‑1 (MQ=60)
                                                                                                                           GGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTG                         <  SRR19973070.3027085/150‑1 (MQ=60)
                                                                                                                                GCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTT                    <  SRR19973070.908834/150‑1 (MQ=60)
                                                                                                                                    GCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTC                <  SRR19973070.1468421/150‑1 (MQ=60)
                                                                                                                                      GAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCAT              <  SRR19973070.2949969/150‑1 (MQ=60)
                                                                                                                                      GAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCAT              <  SRR19973070.2670486/150‑1 (MQ=60)
                                                                                                                                       AACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATG             >  SRR19973070.1135512/1‑150 (MQ=60)
                                                                                                                                           CGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAG         <  SRR19973070.2534382/150‑1 (MQ=60)
                                                                                                                                           CGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAG         <  SRR19973070.1483658/150‑1 (MQ=60)
                                                                                                                                           CGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAG         >  SRR19973070.1862804/1‑150 (MQ=60)
                                                                                                                                              TCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATT      <  SRR19973070.3179931/150‑1 (MQ=60)
                                                                                                                                              TCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATT      <  SRR19973070.2935649/150‑1 (MQ=60)
                                                                                                                                               CAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTT     <  SRR19973070.2818322/150‑1 (MQ=60)
                                                                                                                                               CAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTT     <  SRR19973070.436197/150‑1 (MQ=60)
                                                                                                                                                 GCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT   <  SRR19973070.1299575/150‑1 (MQ=60)
                                                                                                                                                 GCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT   <  SRR19973070.1163940/150‑1 (MQ=60)
                                                                                                                                                 GCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT   <  SRR19973070.1163765/150‑1 (MQ=60)
                                                                                                                                                  CTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCTTGGGAGATTTTTT  >  SRR19973070.2994678/1‑150 (MQ=60)
                                                                                                                                                  CTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTT  <  SRR19973070.2365906/150‑1 (MQ=60)
                                                                                                                                                  CTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTT  <  SRR19973070.1801883/150‑1 (MQ=60)
                                                                                                                                                  CTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTTCAATCGCGTTATGCTGCGCTTTTTCAGGGGAGATTTTTT  >  SRR19973070.683570/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
CAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTT  >  CP009273/4245390‑4245685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑