Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,245,537 G→T 91.4% N286K (AAC→AAA)  plsB ← glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,245,5370GT91.4% 526.3 / 43.3 199N286K (AAC→AAA) plsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (9/8);  new base T (67/115);  total (76/123)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 203 total aligned reads displayed.

GGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATT  >  CP009273/4245392‑4245681
                                                                                                                                                 |                                                                                                                                                
ggTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTTATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCttt                                                                                                                                                <  2:810620/148‑1 (MQ=255)
ggTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCttt                                                                                                                                                >  2:2887703/1‑148 (MQ=255)
  ttGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTAt                                                                                                                                              <  2:2433909/148‑1 (MQ=255)
   tGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATg                                                                                                                                             >  1:386502/1‑148 (MQ=255)
    gATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATgg                                                                                                                                            <  2:2345945/148‑1 (MQ=255)
    gATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATgg                                                                                                                                            <  1:84356/148‑1 (MQ=255)
     aTCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGa                                                                                                                                           <  1:3427421/148‑1 (MQ=255)
     aTCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGa                                                                                                                                           <  2:3414938/148‑1 (MQ=255)
          ggcggcGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTg                                                                                                                                      <  1:3324191/148‑1 (MQ=255)
              gcgATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGc                                                                                                                                  >  1:391646/1‑148 (MQ=255)
               cgATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGcg                                                                                                                                 <  1:2841373/148‑2 (MQ=255)
                 atatGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCt                                                                                                                               <  2:2614201/148‑1 (MQ=255)
                 atatGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCt                                                                                                                               <  2:2812572/148‑1 (MQ=255)
                 atatGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCt                                                                                                                               >  1:2548359/1‑148 (MQ=255)
                  tatGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTg                                                                                                                              <  2:1288123/148‑1 (MQ=255)
                      cggcggCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCGAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTaa                                                                                                                          <  2:2255764/148‑1 (MQ=255)
                       ggcggcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTaaa                                                                                                                         <  2:1908844/148‑1 (MQ=255)
                           gcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCg                                                                                                                     <  1:3089811/148‑1 (MQ=255)
                            cACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCgg                                                                                                                    >  2:1621402/1‑148 (MQ=255)
                            cACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCAAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCgg                                                                                                                    <  2:2619646/148‑1 (MQ=255)
                                aGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTcc                                                                                                                <  1:1918855/148‑1 (MQ=255)
                                 gCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCa                                                                                                               <  2:1391383/148‑1 (MQ=255)
                                 gCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCa                                                                                                               <  2:1091622/148‑1 (MQ=255)
                                  ccccTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAg                                                                                                              >  1:844427/1‑148 (MQ=255)
                                     cTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTg                                                                                                           <  1:3151878/148‑1 (MQ=255)
                                      tGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCatgctc                                                                                                          >  1:1405953/1‑143 (MQ=255)
                                      tGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGa                                                                                                          <  2:482829/148‑1 (MQ=255)
                                        gTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAg                                                                                                        >  1:1251317/1‑148 (MQ=255)
                                          gATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGcc                                                                                                      >  1:1690977/1‑148 (MQ=255)
                                          gATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGcc                                                                                                      >  1:2440289/1‑148 (MQ=255)
                                           aTACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGccc                                                                                                     <  1:3139749/148‑1 (MQ=255)
                                             aCAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAg                                                                                                   >  1:1146494/1‑148 (MQ=255)
                                              cAGCACGTAAGAAAGCAGCAGGTAGTCCTTGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGa                                                                                                  <  2:408489/148‑1 (MQ=255)
                                               aGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGaa                                                                                                 <  1:2906917/148‑1 (MQ=255)
                                                 cACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATa                                                                                               <  1:3480144/148‑1 (MQ=255)
                                                  aCGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATAc                                                                                              <  2:1865999/148‑1 (MQ=255)
                                                      aaGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtc                                                                                          <  1:1911216/148‑1 (MQ=255)
                                                        gAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcag                                                                                        >  1:157941/1‑148 (MQ=255)
                                                             cagcagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAgg                                                                                   <  2:2556383/148‑1 (MQ=255)
                                                                  ggTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAat                                                                              <  2:1545129/148‑1 (MQ=255)
                                                                    tAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatca                                                                            <  2:1636588/148‑1 (MQ=255)
                                                                    tAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatca                                                                            <  1:3455937/148‑1 (MQ=255)
                                                                    tAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatca                                                                            <  1:2216409/148‑1 (MQ=255)
                                                                     aGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatcat                                                                           <  1:682203/148‑1 (MQ=255)
                                                                       tCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCAtct                                                                         >  1:361054/1‑148 (MQ=255)
                                                                          aTGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGt                                                                      >  2:3503901/1‑148 (MQ=255)
                                                                           tgtgACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTa                                                                     >  2:3297615/1‑148 (MQ=255)
                                                                               aCTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAagag                                                                 <  1:268217/148‑1 (MQ=255)
                                                                                cTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAgaga                                                                >  2:2999515/1‑148 (MQ=255)
                                                                                 tGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGaa                                                               <  1:2755382/148‑1 (MQ=255)
                                                                                 tGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGaa                                                               <  1:2755606/148‑1 (MQ=255)
                                                                                   cGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAAt                                                             <  2:232193/148‑1 (MQ=255)
                                                                                   cGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAAt                                                             <  1:2314756/148‑1 (MQ=255)
                                                                                    ggTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAAtt                                                            <  2:14221/148‑1 (MQ=255)
                                                                                      tGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcg                                                          <  1:2439474/148‑1 (MQ=255)
                                                                                           aGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCa                                                     <  2:2291888/148‑1 (MQ=255)
                                                                                           aGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCa                                                     <  2:2273364/148‑1 (MQ=255)
                                                                                             gCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt                                                   >  1:1470253/1‑148 (MQ=255)
                                                                                             gCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt                                                   <  2:2979796/148‑1 (MQ=255)
                                                                                             gCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt                                                   >  1:87106/1‑148 (MQ=255)
                                                                                             gCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt                                                   >  2:1386623/1‑148 (MQ=255)
                                                                                                caTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAtctc                                                <  1:2660528/148‑1 (MQ=255)
                                                                                                caTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAtctc                                                >  1:2174976/1‑148 (MQ=255)
                                                                                                  tataCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCtt                                              >  2:2896613/1‑148 (MQ=255)
                                                                                                   ataCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTc                                             <  2:1323597/148‑1 (MQ=255)
                                                                                                   ataCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTc                                             >  1:2656652/1‑148 (MQ=255)
                                                                                                       cAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATc                                         <  1:3244571/148‑1 (MQ=255)
                                                                                                        aGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCa                                        <  2:1212891/148‑1 (MQ=255)
                                                                                                         gCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAg                                       <  2:386502/148‑1 (MQ=255)
                                                                                                         gCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAg                                       <  2:380895/148‑1 (MQ=255)
                                                                                                          cTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGt                                      >  1:2625308/1‑148 (MQ=255)
                                                                                                           tCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAGGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTg                                     >  2:3060780/1‑148 (MQ=255)
                                                                                                           tCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTg                                     <  1:722902/148‑1 (MQ=255)
                                                                                                              tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCaa                                  <  2:2382929/148‑1 (MQ=255)
                                                                                                              tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCaa                                  <  2:2832776/148‑1 (MQ=255)
                                                                                                              tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCaa                                  <  1:2533927/148‑1 (MQ=255)
                                                                                                               gACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAAt                                 >  1:1320157/1‑148 (MQ=255)
                                                                                                               gACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAAt                                 <  1:2225081/148‑1 (MQ=255)
                                                                                                               gACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAAt                                 <  1:2942971/148‑1 (MQ=255)
                                                                                                               gACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAAt                                 <  2:2987825/148‑1 (MQ=255)
                                                                                                                   gtcgtGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg                             <  2:3382091/148‑1 (MQ=255)
                                                                                                                    tcgtGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGt                            <  1:229028/148‑1 (MQ=255)
                                                                                                                     cgtGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGtt                           >  1:3001574/1‑148 (MQ=255)
                                                                                                                         ggCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctgc                       <  1:3073506/148‑1 (MQ=255)
                                                                                                                               cTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCt                 >  1:3367048/1‑148 (MQ=255)
                                                                                                                                    gAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTc            <  2:2332641/148‑1 (MQ=255)
                                                                                                                                     aaCGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCa           <  2:2637004/148‑1 (MQ=255)
                                                                                                                                      aCGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCGGCGCTTTTTCAt          >  1:2325072/1‑148 (MQ=255)
                                                                                                                                       cgcgCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATg         >  1:1085694/1‑148 (MQ=255)
                                                                                                                                         cgcTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATggg       >  2:1957381/1‑148 (MQ=255)
                                                                                                                                         cgcTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATggg       <  1:3422642/148‑1 (MQ=255)
                                                                                                                                           cTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGgag     >  2:94562/1‑148 (MQ=255)
                                                                                                                                            tCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGgaga    >  1:492629/1‑148 (MQ=255)
                                                                                                                                            tCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGgaga    >  1:3060941/1‑148 (MQ=255)
                                                                                                                                             cAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAt   <  1:1870234/148‑1 (MQ=255)
                                                                                                                                             cAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAt   >  2:2122962/1‑148 (MQ=255)
                                                                                                                                             cAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAt   >  2:2121634/1‑148 (MQ=255)
                                                                                                                                             cAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAt   <  2:1718010/148‑1 (MQ=255)
                                                                                                                                             cAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAt   <  1:804477/148‑1 (MQ=255)
                                                                                                                                             cAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAt   <  2:943216/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATT  >  CP009273/4245392‑4245681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT  >  CP009273/4245389‑4245684
                                                                                                                                                    |                                                                                                                                                   
TCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTT                                                                                                                                                    <  SRR19973067.976811/150‑1 (MQ=60)
TCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTT                                                                                                                                                    <  SRR19973067.976775/150‑1 (MQ=60)
TCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTT                                                                                                                                                    <  SRR19973067.975465/150‑1 (MQ=60)
TCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTT                                                                                                                                                    <  SRR19973067.1455529/150‑1 (MQ=60)
TCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTT                                                                                                                                                    <  SRR19973067.1455204/150‑1 (MQ=60)
   GGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTAT                                                                                                                                                 <  SRR19973067.3275559/150‑1 (MQ=60)
    GTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGACCGCGCTCAGCGTTATG                                                                                                                                                >  SRR19973067.389200/1‑150 (MQ=60)
    GTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATG                                                                                                                                                >  SRR19973067.389299/1‑150 (MQ=60)
    GTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATG                                                                                                                                                >  SRR19973067.383551/1‑150 (MQ=60)
       GATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGAC                                                                                                                                             <  SRR19973067.85277/150‑1 (MQ=60)
       GATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGAC                                                                                                                                             <  SRR19973067.85285/150‑1 (MQ=60)
        ATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACG                                                                                                                                            <  SRR19973067.3449234/150‑1 (MQ=60)
          CCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTT                                                                                                                                          >  SRR19973067.3055250/1‑150 (MQ=60)
            CGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGA                                                                                                                                        >  SRR19973067.2170709/1‑150 (MQ=60)
             GGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGAT                                                                                                                                       <  SRR19973067.3345218/150‑1 (MQ=60)
               CGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGC                                                                                                                                     >  SRR19973067.394389/1‑150 (MQ=60)
                  CGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCGCT                                                                                                                                  <  SRR19973067.2859617/150‑1 (MQ=60)
                  CGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCT                                                                                                                                  >  SRR19973067.2565031/1‑150 (MQ=60)
                              GCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGT                                                                                                                      <  SRR19973067.1329323/150‑1 (MQ=60)
                              GCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGT                                                                                                                      <  SRR19973067.3109469/150‑1 (MQ=60)
                                 CCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCC                                                                                                                   <  SRR19973067.3098563/150‑1 (MQ=60)
                                   AGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAG                                                                                                                 >  SRR19973067.850147/1‑150 (MQ=60)
                                   AGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAG                                                                                                                 <  SRR19973067.1931947/150‑1 (MQ=60)
                                    GCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGG                                                                                                                <  SRR19973067.1258368/150‑1 (MQ=60)
                                       CCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAtgctc                                                                                                             >  SRR19973067.1415486/1‑145 (MQ=60)
                                        CTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAA                                                                                                            >  SRR19973067.930689/1‑150 (MQ=60)
                                        CTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAA                                                                                                            <  SRR19973067.3172045/150‑1 (MQ=60)
                                        CTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAA                                                                                                            <  SRR19973067.3171861/150‑1 (MQ=60)
                                         TGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAG                                                                                                           >  SRR19973067.833054/1‑150 (MQ=60)
                                         TGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAG                                                                                                           >  SRR19973067.1259772/1‑150 (MQ=60)
                                         TGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAG                                                                                                           <  SRR19973067.1318357/150‑1 (MQ=60)
                                           GTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCC                                                                                                         >  SRR19973067.1702616/1‑150 (MQ=60)
                                           GTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCC                                                                                                         >  SRR19973067.2456313/1‑150 (MQ=60)
                                           GTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGCTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCC                                                                                                         >  SRR19973067.2456291/1‑150 (MQ=60)
                                            TGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCC                                                                                                        >  SRR19973067.1804114/1‑150 (MQ=60)
                                              ATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAG                                                                                                      <  SRR19973067.3159685/150‑1 (MQ=60)
                                              ATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAG                                                                                                      <  SRR19973067.2663888/150‑1 (MQ=60)
                                              ATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAG                                                                                                      >  SRR19973067.1154178/1‑150 (MQ=60)
                                                 CAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAAT                                                                                                   <  SRR19973067.780824/150‑1 (MQ=60)
                                                  AGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATA                                                                                                  <  SRR19973067.2925464/150‑1 (MQ=60)
                                                  AGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATA                                                                                                  <  SRR19973067.2925440/150‑1 (MQ=60)
                                                    CACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACG                                                                                                <  SRR19973067.3502231/150‑1 (MQ=60)
                                                         AAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAG                                                                                           >  SRR19973067.3472585/1‑150 (MQ=60)
                                                         AAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAG                                                                                           >  SRR19973067.159258/1‑150 (MQ=60)
                                                         AAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAG                                                                                           <  SRR19973067.875535/150‑1 (MQ=60)
                                                         AAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAG                                                                                           >  SRR19973067.2406913/1‑150 (MQ=60)
                                                         AAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAG                                                                                           >  SRR19973067.608912/1‑150 (MQ=60)
                                                         AAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAG                                                                                           <  SRR19973067.1924254/150‑1 (MQ=60)
                                                         AAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAG                                                                                           <  SRR19973067.1924258/150‑1 (MQ=60)
                                                                 AGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGA                                                                                   >  SRR19973067.3168932/1‑150 (MQ=60)
                                                                   CAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAAT                                                                                 <  SRR19973067.2565609/150‑1 (MQ=60)
                                                                   CAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAAT                                                                                 <  SRR19973067.3003350/150‑1 (MQ=60)
                                                                    AGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATC                                                                                <  SRR19973067.267705/150‑1 (MQ=60)
                                                                       TAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATC                                                                             <  SRR19973067.2231170/150‑1 (MQ=60)
                                                                       TAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATC                                                                             <  SRR19973067.2231245/150‑1 (MQ=60)
                                                                       TAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATC                                                                             <  SRR19973067.3477918/150‑1 (MQ=60)
                                                                        AGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCT                                                                            <  SRR19973067.686892/150‑1 (MQ=60)
                                                                        AGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCT                                                                            >  SRR19973067.363557/1‑150 (MQ=60)
                                                                                  ACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAA                                                                  <  SRR19973067.270023/150‑1 (MQ=60)
                                                                                  ACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAA                                                                  <  SRR19973067.2493990/150‑1 (MQ=60)
                                                                                    TGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAANT                                                                <  SRR19973067.2773319/150‑1 (MQ=60)
                                                                                    TGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAAT                                                                <  SRR19973067.2773188/150‑1 (MQ=60)
                                                                                    TGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAAT                                                                <  SRR19973067.2773413/150‑1 (MQ=60)
                                                                                      CGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTC                                                              <  SRR19973067.2331355/150‑1 (MQ=60)
                                                                                      CGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTC                                                              <  SRR19973067.2329989/150‑1 (MQ=60)
                                                                                         TGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCC                                                           <  SRR19973067.2455495/150‑1 (MQ=60)
                                                                                             AAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAA                                                       >  SRR19973067.694540/1‑150 (MQ=60)
                                                                                              AGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAT                                                      >  SRR19973067.88048/1‑150 (MQ=60)
                                                                                              AGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAT                                                      >  SRR19973067.1480050/1‑150 (MQ=60)
                                                                                                 CACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTC                                                   >  SRR19973067.2189519/1‑150 (MQ=60)
                                                                                                 CACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTC                                                   >  SRR19973067.2189596/1‑150 (MQ=60)
                                                                                                   CATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTT                                                 <  SRR19973067.2677645/150‑1 (MQ=60)
                                                                                                   CATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTT                                                 <  SRR19973067.2677680/150‑1 (MQ=60)
                                                                                                    ATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTC                                                >  SRR19973067.2673757/1‑150 (MQ=60)
                                                                                                         CCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCA                                           <  SRR19973067.3573012/150‑1 (MQ=60)
                                                                                                          CAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAG                                          <  SRR19973067.3264895/150‑1 (MQ=60)
                                                                                                           AGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGT                                         >  SRR19973067.2642327/1‑150 (MQ=60)
                                                                                                              TCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCA                                      <  SRR19973067.1570010/150‑1 (MQ=60)
                                                                                                              TCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCA                                      <  SRR19973067.727926/150‑1 (MQ=60)
                                                                                                                ATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAAT                                    >  SRR19973067.3366755/1‑150 (MQ=60)
                                                                                                                ATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAAT                                    >  SRR19973067.1329167/1‑150 (MQ=60)
                                                                                                                 TGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATC                                   <  SRR19973067.2550530/150‑1 (MQ=60)
                                                                                                                 TGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATC                                   <  SRR19973067.2550531/150‑1 (MQ=60)
                                                                                                                  GACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCG                                  <  SRR19973067.2961618/150‑1 (MQ=60)
                                                                                                                  GACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCG                                  <  SRR19973067.2239892/150‑1 (MQ=60)
                                                                                                                  GACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCG                                  <  SRR19973067.2240079/150‑1 (MQ=60)
                                                                                                                      GTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTT                              >  SRR19973067.3020627/1‑150 (MQ=60)
                                                                                                                       TCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTC                             <  SRR19973067.230670/150‑1 (MQ=60)
                                                                                                                       TCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTC                             <  SRR19973067.230734/150‑1 (MQ=60)
                                                                                                                           GGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCT                         <  SRR19973067.419863/150‑1 (MQ=60)
                                                                                                                            GGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTG                        <  SRR19973067.3093074/150‑1 (MQ=60)
                                                                                                                            GGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTG                        <  SRR19973067.3093065/150‑1 (MQ=60)
                                                                                                                            GGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTG                        >  SRR19973067.1607560/1‑150 (MQ=60)
                                                                                                                               CAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGC                     >  SRR19973067.1101886/1‑150 (MQ=60)
                                                                                                                               CAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGC                     >  SRR19973067.3134538/1‑150 (MQ=60)
                                                                                                                                AGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCT                    >  SRR19973067.3388424/1‑150 (MQ=60)
                                                                                                                                 GCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTT                   <  SRR19973067.2876267/150‑1 (MQ=60)
                                                                                                                                      CGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCA              <  SRR19973067.2740796/150‑1 (MQ=60)
                                                                                                                                       GAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCAT             <  SRR19973067.3123352/150‑1 (MQ=60)
                                                                                                                                       GAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCGGCGCTTTTTCAT             >  SRR19973067.2340345/1‑150 (MQ=60)
                                                                                                                                        AACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATG            >  SRR19973067.1093120/1‑150 (MQ=60)
                                                                                                                                            CGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAG        <  SRR19973067.3444482/150‑1 (MQ=60)
                                                                                                                                            CGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAG        <  SRR19973067.3444424/150‑1 (MQ=60)
                                                                                                                                             GCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGA       >  SRR19973067.496015/1‑150 (MQ=60)
                                                                                                                                             GCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGA       >  SRR19973067.3080429/1‑150 (MQ=60)
                                                                                                                                              CTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAT      >  SRR19973067.2857302/1‑150 (MQ=60)
                                                                                                                                               TCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATT     >  SRR19973067.1445964/1‑150 (MQ=60)
                                                                                                                                               TCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATT     <  SRR19973067.3488950/150‑1 (MQ=60)
                                                                                                                                                CAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTT    <  SRR19973067.1882989/150‑1 (MQ=60)
                                                                                                                                                CAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTT    <  SRR19973067.810012/150‑1 (MQ=60)
                                                                                                                                                CAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTT    <  SRR19973067.1883571/150‑1 (MQ=60)
                                                                                                                                                 AGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTT   <  SRR19973067.3437681/150‑1 (MQ=60)
                                                                                                                                                 AGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTT   >  SRR19973067.2280364/1‑150 (MQ=60)
                                                                                                                                                 AGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTT   >  SRR19973067.2280063/1‑150 (MQ=60)
                                                                                                                                                 AGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTT   >  SRR19973067.2136615/1‑150 (MQ=60)
                                                                                                                                                  GCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT  <  SRR19973067.472435/150‑1 (MQ=60)
                                                                                                                                                  GCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT  <  SRR19973067.3528104/150‑1 (MQ=60)
                                                                                                                                                  GCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT  <  SRR19973067.3527931/150‑1 (MQ=60)
                                                                                                                                                  GCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTGCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT  <  SRR19973067.3081745/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
TCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT  >  CP009273/4245389‑4245684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑