Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,245,537 G→T 100% N286K (AAC→AAA)  plsB ← glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,245,5370GT97.4% 591.9 / 6.4 192N286K (AAC→AAA) plsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (76/111);  total (77/115)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

GGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAT  >  CP009273/4245392‑4245680
                                                                                                                                                 |                                                                                                                                               
ggTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCttt                                                                                                                                               <  1:2112690/148‑1 (MQ=255)
ggTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGGGGGCCAGCTGGCGAACGCGCTCAGCttt                                                                                                                                               >  1:401654/1‑148 (MQ=255)
   tGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATg                                                                                                                                            <  1:2364289/148‑1 (MQ=255)
    gATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATgg                                                                                                                                           <  2:1057620/148‑1 (MQ=255)
     aTCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGa                                                                                                                                          <  1:2176097/148‑1 (MQ=255)
     aTCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGa                                                                                                                                          >  2:2762366/1‑148 (MQ=255)
     aTCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGa                                                                                                                                          <  1:2002458/148‑1 (MQ=255)
      tCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGAc                                                                                                                                         >  2:400107/1‑148 (MQ=255)
      tCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGAc                                                                                                                                         >  2:1137431/1‑148 (MQ=255)
       cccGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACg                                                                                                                                        <  1:1241040/148‑1 (MQ=255)
           gcggcgATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGa                                                                                                                                    >  2:1613352/1‑148 (MQ=255)
           gcggcgATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGa                                                                                                                                    >  2:1613739/1‑148 (MQ=255)
             ggcgATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATg                                                                                                                                  <  1:2950959/148‑1 (MQ=255)
                  tatGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTg                                                                                                                             <  2:777701/148‑1 (MQ=255)
                      cggcggCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTaa                                                                                                                         <  1:1465916/148‑1 (MQ=255)
                         cggcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGt                                                                                                                      >  1:951655/1‑148 (MQ=255)
                           gcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGACCGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCg                                                                                                                    >  2:2605565/1‑148 (MQ=255)
                           gcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCg                                                                                                                    <  1:2011421/148‑1 (MQ=255)
                           gcACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCg                                                                                                                    >  2:818834/1‑148 (MQ=255)
                            cACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCgg                                                                                                                   >  1:742694/1‑148 (MQ=255)
                             aCCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGt                                                                                                                  <  2:613988/148‑1 (MQ=255)
                               cAGCCCCTGGTGATACAGCACGTAAGAAAGCAGAAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGTCCAGCTGGCGAACGCGCTCAGCTTTAAGGACGTTGATGCCCTGGTAAAGTCGGTTc                                                                                                                >  2:1857986/1‑148 (MQ=255)
                                aGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTcc                                                                                                               <  2:414232/148‑1 (MQ=255)
                                         tGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGc                                                                                                      <  1:272694/148‑1 (MQ=255)
                                          gATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGcc                                                                                                     <  2:2356218/148‑1 (MQ=255)
                                           aTACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGccc                                                                                                    >  2:174482/1‑148 (MQ=255)
                                           aTACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGccc                                                                                                    >  1:3511841/1‑148 (MQ=255)
                                           aTACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGccc                                                                                                    >  1:3511681/1‑148 (MQ=255)
                                               aGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGaa                                                                                                >  2:1198284/1‑148 (MQ=255)
                                                gCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAAt                                                                                               >  1:1180370/1‑148 (MQ=255)
                                                 cACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATa                                                                                              >  2:3142241/1‑148 (MQ=255)
                                                    gTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACgg                                                                                           <  2:1174667/148‑1 (MQ=255)
                                                    gTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGAGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACgg                                                                                           <  1:3236623/148‑1 (MQ=255)
                                                      aaGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtc                                                                                         >  1:3100387/1‑148 (MQ=255)
                                                      aaGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtc                                                                                         <  2:642536/148‑1 (MQ=255)
                                                         aaaGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGgtcagt                                                                                      <  1:2587513/148‑1 (MQ=255)
                                                             cagcagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAgg                                                                                  >  2:3206023/1‑148 (MQ=255)
                                                                cagGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGa                                                                               >  1:2467267/1‑148 (MQ=255)
                                                                  ggTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGACGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAat                                                                             >  1:3174075/1‑148 (MQ=255)
                                                                   gTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatc                                                                            <  1:1945909/148‑1 (MQ=255)
                                                                    gaGTCCATGGGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatca                                                                           <  1:1845940/147‑1 (MQ=255)
                                                                     aGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGCCCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTATAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCTGTCAGGCGAatcat                                                                          <  2:2111270/148‑1 (MQ=255)
                                                                     aGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatcat                                                                          <  1:2629426/148‑1 (MQ=255)
                                                                     aGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatcat                                                                          <  1:842468/148‑1 (MQ=255)
                                                                     aGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatcat                                                                          <  2:1023035/148‑1 (MQ=255)
                                                                      gTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAatcatc                                                                         <  1:1137431/148‑1 (MQ=255)
                                                                         cATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCg                                                                      <  2:2021950/148‑1 (MQ=255)
                                                                         cATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCg                                                                      <  1:3359204/148‑1 (MQ=255)
                                                                               aCTGCTGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAagag                                                                <  2:2428284/148‑1 (MQ=255)
                                                                               aCTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAagag                                                                >  1:2322152/1‑148 (MQ=255)
                                                                               aCTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAagag                                                                <  2:844711/148‑1 (MQ=255)
                                                                                 tGCGGTGGCAAGGCACATAGACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGaa                                                              <  2:2562690/148‑1 (MQ=255)
                                                                                  gCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGaaa                                                             >  2:2917106/1‑148 (MQ=255)
                                                                                   cGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAAt                                                            <  2:193449/148‑1 (MQ=255)
                                                                                         cAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgccg                                                      >  2:2181911/1‑148 (MQ=255)
                                                                                         cAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTcgccg                                                      >  2:1579262/1‑148 (MQ=255)
                                                                                           aGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCa                                                    <  2:3390407/148‑1 (MQ=255)
                                                                                           aGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCa                                                    <  2:3390729/148‑1 (MQ=255)
                                                                                            ggCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCaa                                                   <  1:2318739/148‑1 (MQ=255)
                                                                                             gCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt                                                  <  1:215915/148‑1 (MQ=255)
                                                                                             gCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAt                                                  <  1:1759172/148‑1 (MQ=255)
                                                                                               acaTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTCTGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGAAACAGACATTCGCCCCAAtct                                                >  2:3175663/1‑148 (MQ=255)
                                                                                                 atatACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATctct                                              <  2:1101198/148‑1 (MQ=255)
                                                                                                   ataCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTc                                            <  2:3223601/148‑1 (MQ=255)
                                                                                                   ataCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTc                                            <  2:729834/148‑1 (MQ=255)
                                                                                                   ataCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTc                                            <  1:174495/148‑1 (MQ=255)
                                                                                                      ccAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCTGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAt                                         <  1:1595418/148‑1 (MQ=255)
                                                                                                      ccAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAt                                         <  1:1898789/148‑1 (MQ=255)
                                                                                                      ccAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAt                                         <  2:2234714/148‑1 (MQ=255)
                                                                                                      ccAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAt                                         >  1:1732103/1‑148 (MQ=255)
                                                                                                      ccAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAt                                         <  2:630774/148‑1 (MQ=255)
                                                                                                         gCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAg                                      >  2:1032759/1‑148 (MQ=255)
                                                                                                           tCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTg                                    >  2:356007/1‑148 (MQ=255)
                                                                                                             aTGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCa                                  <  2:724670/148‑1 (MQ=255)
                                                                                                              tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCaa                                 <  2:6448/148‑1 (MQ=255)
                                                                                                              tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCaa                                 <  1:866480/148‑1 (MQ=255)
                                                                                                              tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCaa                                 <  2:7158/148‑1 (MQ=255)
                                                                                                              tGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCaa                                 <  1:1109563/148‑1 (MQ=255)
                                                                                                               gACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAAt                                <  1:1647698/148‑1 (MQ=255)
                                                                                                                   gtcgtGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATcgcg                            <  2:2666962/148‑1 (MQ=255)
                                                                                                                     cgtGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGTAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGtt                          <  2:951651/148‑1 (MQ=255)
                                                                                                                      gtGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTc                         <  2:1696980/148‑1 (MQ=255)
                                                                                                                        gggCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctg                       <  1:3409508/148‑1 (MQ=255)
                                                                                                                        gggCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctg                       <  1:609386/148‑1 (MQ=255)
                                                                                                                        gggCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTctg                       <  1:623650/148‑1 (MQ=255)
                                                                                                                             aGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGTCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcg                  <  2:1235053/148‑1 (MQ=255)
                                                                                                                             aGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcg                  <  1:1448150/148‑1 (MQ=255)
                                                                                                                             aGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcg                  >  2:2400880/1‑148 (MQ=255)
                                                                                                                             aGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcg                  <  1:1188970/148‑1 (MQ=255)
                                                                                                                             aGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTgcg                  <  1:1654882/148‑1 (MQ=255)
                                                                                                                               cTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCt                <  2:1460208/148‑1 (MQ=255)
                                                                                                                                  gCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCtttt             <  1:2762366/148‑1 (MQ=255)
                                                                                                                                   cGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCttttt            <  1:504472/148‑1 (MQ=255)
                                                                                                                                      aCGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCAt         >  2:2002236/1‑148 (MQ=255)
                                                                                                                                           cTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGgag    >  2:2517551/1‑148 (MQ=255)
                                                                                                                                           cTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGgag    >  2:2540362/1‑148 (MQ=255)
                                                                                                                                           cTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGgag    >  1:2038547/1‑148 (MQ=255)
                                                                                                                                            tCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGgaga   <  2:2786085/148‑1 (MQ=255)
                                                                                                                                             cAGCTTTATGGACTTTGATGCGCTGGTAAAGTCGGTTCCAGGTGTAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCCTGGGAGAt  <  1:3440283/148‑1 (MQ=255)
                                                                                                                                             cAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAt  <  2:1807710/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGAT  >  CP009273/4245392‑4245680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTT  >  CP009273/4245389‑4245686
                                                                                                                                                    |                                                                                                                                                     
TCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTT                                                                                                                                                      <  SRR19973066.1270995/150‑1 (MQ=60)
 CAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTT                                                                                                                                                     >  SRR19973066.1865840/1‑150 (MQ=60)
 CAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTT                                                                                                                                                     >  SRR19973066.2522642/1‑150 (MQ=60)
 CAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGGGGGCCAGCTGGCGAACGCGCTCAGCTTT                                                                                                                                                     >  SRR19973066.404817/1‑150 (MQ=60)
  AGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTA                                                                                                                                                    >  SRR19973066.2084442/1‑150 (MQ=60)
   GGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTAT                                                                                                                                                   <  SRR19973066.2127290/150‑1 (MQ=60)
    GTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATG                                                                                                                                                  >  SRR19973066.1979064/1‑150 (MQ=60)
      TGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGA                                                                                                                                                <  SRR19973066.2380301/150‑1 (MQ=60)
       GATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGAC                                                                                                                                               <  SRR19973066.1955263/150‑1 (MQ=60)
        ATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACG                                                                                                                                              <  SRR19973066.2191210/150‑1 (MQ=60)
        ATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACG                                                                                                                                              <  SRR19973066.2016522/150‑1 (MQ=60)
        ATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACG                                                                                                                                              <  SRR19973066.2017156/150‑1 (MQ=60)
          CCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTT                                                                                                                                            <  SRR19973066.2880649/150‑1 (MQ=60)
          CCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTT                                                                                                                                            <  SRR19973066.1249917/150‑1 (MQ=60)
                GGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCC                                                                                                                                      <  SRR19973066.2970539/150‑1 (MQ=60)
                    ATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGG                                                                                                                                  >  SRR19973066.1470544/1‑150 (MQ=60)
                       TGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAA                                                                                                                               <  SRR19973066.1609675/150‑1 (MQ=60)
                         CGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAG                                                                                                                             <  SRR19973066.1476261/150‑1 (MQ=60)
                          GGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGT                                                                                                                            >  SRR19973066.958607/1‑150 (MQ=60)
                          GGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGT                                                                                                                            >  SRR19973066.958611/1‑150 (MQ=60)
                            CGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCG                                                                                                                          <  SRR19973066.2213364/150‑1 (MQ=60)
                             GGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGG                                                                                                                         >  SRR19973066.748207/1‑150 (MQ=60)
                              GCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGT                                                                                                                        <  SRR19973066.2025524/150‑1 (MQ=60)
                              GCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGT                                                                                                                        <  SRR19973066.2025514/150‑1 (MQ=60)
                                 CCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCC                                                                                                                     <  SRR19973066.1498507/150‑1 (MQ=60)
                                     CCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGT                                                                                                                 >  SRR19973066.2770182/1‑150 (MQ=60)
                                        CTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAA                                                                                                              >  SRR19973066.1709312/1‑150 (MQ=60)
                                            TGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCC                                                                                                          >  SRR19973066.1191818/1‑150 (MQ=60)
                                            TGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCC                                                                                                          >  SRR19973066.3535233/1‑150 (MQ=60)
                                            TGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCC                                                                                                          >  SRR19973066.3535075/1‑150 (MQ=60)
                                            TGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCC                                                                                                          <  SRR19973066.274706/150‑1 (MQ=60)
                                            TGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCC                                                                                                          >  SRR19973066.3535072/1‑150 (MQ=60)
                                            TGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTATATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCC                                                                                                          >  SRR19973066.450719/1‑150 (MQ=60)
                                              ATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAG                                                                                                        >  SRR19973066.2147637/1‑150 (MQ=60)
                                                 CAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAAT                                                                                                     >  SRR19973066.1188670/1‑150 (MQ=60)
                                                  AGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATA                                                                                                    >  SRR19973066.2188824/1‑150 (MQ=60)
                                                       GTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTC                                                                                               >  SRR19973066.3120965/1‑150 (MQ=60)
                                                       GTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTC                                                                                               >  SRR19973066.3121095/1‑150 (MQ=60)
                                                       GTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGAGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTC                                                                                               <  SRR19973066.3257990/150‑1 (MQ=60)
                                                            AAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCA                                                                                          <  SRR19973066.2605050/150‑1 (MQ=60)
                                                                CAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCG                                                                                      <  SRR19973066.1763097/150‑1 (MQ=60)
                                                                 AGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGA                                                                                     >  SRR19973066.2484089/1‑150 (MQ=60)
                                                                   CAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGACGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAAT                                                                                   >  SRR19973066.3195209/1‑150 (MQ=60)
                                                                      GTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCAT                                                                                <  SRR19973066.1959691/150‑1 (MQ=60)
                                                                       GAGTCCATGGGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATC                                                                               <  SRR19973066.1859145/150‑1 (MQ=60)
                                                                        AGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCT                                                                              <  SRR19973066.848586/150‑1 (MQ=60)
                                                                        AGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCT                                                                              <  SRR19973066.2647144/150‑1 (MQ=60)
                                                                         GTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTC                                                                             <  SRR19973066.1145592/150‑1 (MQ=60)
                                                                         GTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTC                                                                             <  SRR19973066.1145574/150‑1 (MQ=60)
                                                                            CATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTA                                                                          <  SRR19973066.3381604/150‑1 (MQ=60)
                                                                             ATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAA                                                                         <  SRR19973066.1264685/150‑1 (MQ=60)
                                                                                TGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAG                                                                      >  SRR19973066.2338015/1‑150 (MQ=60)
                                                                                     GCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATT                                                                 <  SRR19973066.3357339/150‑1 (MQ=60)
                                                                                              AGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTGTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAAT                                                        >  SRR19973066.1206667/1‑150 (MQ=60)
                                                                                               GGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATC                                                       <  SRR19973066.2334592/150‑1 (MQ=60)
                                                                                                GCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCT                                                      <  SRR19973066.217690/150‑1 (MQ=60)
                                                                                                GCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCT                                                      <  SRR19973066.1771884/150‑1 (MQ=60)
                                                                                                GCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCT                                                      <  SRR19973066.1771807/150‑1 (MQ=60)
                                                                                                      ATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCA                                                <  SRR19973066.176027/150‑1 (MQ=60)
                                                                                                      ATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCA                                                <  SRR19973066.176040/150‑1 (MQ=60)
                                                                                                       TACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCAT                                               >  SRR19973066.1744614/1‑150 (MQ=60)
                                                                                                         CCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCA                                             <  SRR19973066.1912337/150‑1 (MQ=60)
                                                                                                         CCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGAGGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCTGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCA                                             <  SRR19973066.1606967/150‑1 (MQ=60)
                                                                                                             CTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGC                                         >  SRR19973066.2349337/1‑150 (MQ=60)
                                                                                                             CTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGC                                         >  SRR19973066.546681/1‑150 (MQ=60)
                                                                                                             CTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGC                                         >  SRR19973066.3210119/1‑150 (MQ=60)
                                                                                                                ATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTGCAGGTGAAGCCCAGAATACGGTAAGTCAGGCGAATCATCTCGTAAGAGAAATTAGCCGCAATCTCTTACATCAGTGCAAT                                      >  SRR19973066.2420402/1‑150 (MQ=60)
                                                                                                                ATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCCAT                                      >  SRR19973066.618410/1‑150 (MQ=60)
                                                                                                                 TGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATC                                     <  SRR19973066.872698/150‑1 (MQ=60)
                                                                                                                 TGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATC                                     <  SRR19973066.870672/150‑1 (MQ=60)
                                                                                                                 TGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATC                                     <  SRR19973066.1117593/150‑1 (MQ=60)
                                                                                                                 TGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATC                                     <  SRR19973066.2667354/150‑1 (MQ=60)
                                                                                                                  GACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCG                                    <  SRR19973066.1659698/150‑1 (MQ=60)
                                                                                                                  GACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCG                                    <  SRR19973066.1659700/150‑1 (MQ=60)
                                                                                                                           GGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCT                           <  SRR19973066.3432274/150‑1 (MQ=60)
                                                                                                                           GGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCT                           <  SRR19973066.3432294/150‑1 (MQ=60)
                                                                                                                           GGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCT                           <  SRR19973066.628106/150‑1 (MQ=60)
                                                                                                                           GGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCT                           <  SRR19973066.613625/150‑1 (MQ=60)
                                                                                                                           GGGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCT                           <  SRR19973066.628096/150‑1 (MQ=60)
                                                                                                                            GGCCAGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTG                          <  SRR19973066.2335968/150‑1 (MQ=60)
                                                                                                                                AGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCT                      <  SRR19973066.1458438/150‑1 (MQ=60)
                                                                                                                                AGCTGGCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCT                      <  SRR19973066.3145709/150‑1 (MQ=60)
                                                                                                                                AGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCT                      <  SRR19973066.3445037/150‑1 (MQ=60)
                                                                                                                                AGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCT                      <  SRR19973066.1197306/150‑1 (MQ=60)
                                                                                                                                AGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCT                      <  SRR19973066.1666940/150‑1 (MQ=60)
                                                                                                                                     GCGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTC                 <  SRR19973066.2780963/150‑1 (MQ=60)
                                                                                                                                      CGAACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCA                <  SRR19973066.508221/150‑1 (MQ=60)
                                                                                                                                        AACGCGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATG              <  SRR19973066.403257/150‑1 (MQ=60)
                                                                                                                                            CGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAG          >  SRR19973066.2052754/1‑150 (MQ=60)
                                                                                                                                            CGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAG          <  SRR19973066.828430/150‑1 (MQ=60)
                                                                                                                                            CGCTCAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAG          <  SRR19973066.2610679/150‑1 (MQ=60)
                                                                                                                                                CAGCTTTATGGACTTTGATGCGCTGGTAAAGTCGGTTCCAGGTGTAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCCTGGGAGATTT      <  SRR19973066.3463296/150‑1 (MQ=60)
                                                                                                                                                CAGCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTT      <  SRR19973066.3463300/150‑1 (MQ=60)
                                                                                                                                                  GCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCTTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT    <  SRR19973066.2304641/150‑1 (MQ=60)
                                                                                                                                                  GCTTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTT    <  SRR19973066.20976/150‑1 (MQ=60)
                                                                                                                                                    TTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTT  <  SRR19973066.1048732/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTT  >  CP009273/4245389‑4245686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑