Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 137,915 G→T 100% intergenic (‑203/‑3) gcd ← / → hpt glucose dehydrogenase/hypoxanthine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273137,9150GT97.7% 542.7 / 2.0 172intergenic (‑203/‑3)gcd/hptglucose dehydrogenase/hypoxanthine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (89/79);  total (92/80)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 177 total aligned reads displayed.

ATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTT  >  CP009273/137771‑138061
                                                                                                                                                |                                                                                                                                                  
aTGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAtata                                                                                                                                                 <  1:1355643/148‑1 (MQ=255)
 tgtgtgATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGatatat                                                                                                                                                <  2:71645/148‑1 (MQ=255)
    gtgATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGaa                                                                                                                                             >  1:1445127/1‑148 (MQ=255)
    gtgATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGaa                                                                                                                                             >  1:720753/1‑148 (MQ=255)
     tgATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGaaa                                                                                                                                            <  2:684413/148‑1 (MQ=255)
        tCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACAt                                                                                                                                         >  2:3115311/1‑148 (MQ=255)
        tCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACAt                                                                                                                                         <  2:1912970/148‑1 (MQ=255)
          gTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATAc                                                                                                                                       <  1:1371481/148‑1 (MQ=255)
           tcatcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACt                                                                                                                                      >  1:1752484/1‑148 (MQ=255)
             atcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGt                                                                                                                                    <  2:320752/148‑1 (MQ=255)
             atcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGt                                                                                                                                    <  1:2234001/148‑1 (MQ=255)
             atcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGt                                                                                                                                    >  1:661852/1‑148 (MQ=255)
             atcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGt                                                                                                                                    <  1:1045220/148‑1 (MQ=255)
             atcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGt                                                                                                                                    <  1:1045429/148‑1 (MQ=255)
              tcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTa                                                                                                                                   >  2:485165/1‑148 (MQ=255)
              tcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTa                                                                                                                                   >  1:2497479/1‑148 (MQ=255)
               cacaATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAg                                                                                                                                  <  2:884676/148‑1 (MQ=255)
               cacaATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAg                                                                                                                                  >  1:493261/1‑148 (MQ=255)
                acaATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGa                                                                                                                                 >  1:204871/1‑148 (MQ=255)
                acaATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGAGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATTAAACATACTGTAGa                                                                                                                                 >  1:2000297/1‑148 (MQ=255)
                    ttCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTa                                                                                                                             <  1:3160709/148‑1 (MQ=255)
                     tCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTaa                                                                                                                            >  1:189847/1‑148 (MQ=255)
                       gAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATg                                                                                                                          <  2:3177915/148‑1 (MQ=255)
                       gAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATg                                                                                                                          <  2:3177910/148‑1 (MQ=255)
                        aGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGa                                                                                                                         <  2:1761925/148‑1 (MQ=255)
                        aGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGa                                                                                                                         <  2:3475764/148‑1 (MQ=255)
                          cTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATc                                                                                                                       <  1:920765/148‑1 (MQ=255)
                           tttATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATcc                                                                                                                      <  2:1476840/148‑1 (MQ=255)
                            ttattaACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATccc                                                                                                                     >  2:1739078/1‑148 (MQ=255)
                                taACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGaa                                                                                                                 >  1:3082040/1‑148 (MQ=255)
                                   cAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCg                                                                                                              >  2:1384665/1‑148 (MQ=255)
                                     gATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGga                                                                                                            >  2:17334/1‑148 (MQ=255)
                                      aTTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGgag                                                                                                           >  2:653482/1‑148 (MQ=255)
                                        tCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGAt                                                                                                         >  2:810899/1‑148 (MQ=255)
                                        tCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGAt                                                                                                         >  2:810922/1‑148 (MQ=255)
                                        tCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTACCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGAt                                                                                                         <  2:3086857/148‑1 (MQ=255)
                                            cgAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTaaa                                                                                                     <  2:1536305/148‑1 (MQ=255)
                                            cgAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTaaa                                                                                                     <  2:2031588/148‑1 (MQ=255)
                                            cgAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTaaa                                                                                                     <  2:2607585/148‑1 (MQ=255)
                                            cgAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTaaa                                                                                                     <  2:2608331/148‑1 (MQ=255)
                                              aatgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgc                                                                                                   >  2:2640056/1‑148 (MQ=255)
                                              aatgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgc                                                                                                   >  2:1187729/1‑148 (MQ=255)
                                                tgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgcgc                                                                                                 >  1:2927075/1‑148 (MQ=255)
                                                    tAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTAt                                                                                             <  2:2325463/148‑1 (MQ=255)
                                                    tAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTAt                                                                                             <  2:1250328/148‑1 (MQ=255)
                                                     aGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATc                                                                                            >  2:1616340/1‑148 (MQ=255)
                                                      gTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCg                                                                                           <  1:158852/148‑1 (MQ=255)
                                                       ttttACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGc                                                                                          >  2:2771245/1‑148 (MQ=255)
                                                        tttACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGcc                                                                                         >  1:644259/1‑148 (MQ=255)
                                                               gTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTgg                                                                                  <  1:1410826/148‑1 (MQ=255)
                                                                tataCTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTggg                                                                                 <  2:3323050/148‑1 (MQ=255)
                                                                  taCTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgtc                                                                               >  1:828403/1‑148 (MQ=255)
                                                                    cTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgtcgt                                                                             >  2:3100309/1‑148 (MQ=255)
                                                                     tGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgtcgtc                                                                            <  1:1103998/148‑1 (MQ=255)
                                                                     tGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgtcgtc                                                                            <  2:1160661/148‑1 (MQ=255)
                                                                            cTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTAc                                                                     >  1:2274762/1‑148 (MQ=255)
                                                                               gcgcTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGa                                                                  >  1:2908386/1‑148 (MQ=255)
                                                                                 gcTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGc                                                                >  1:1603766/1‑148 (MQ=255)
                                                                                  cTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCg                                                               >  2:1382303/1‑148 (MQ=255)
                                                                                         gCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACaaa                                                        >  1:151329/1‑148 (MQ=255)
                                                                                         gCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACaaa                                                        <  1:1639973/148‑1 (MQ=255)
                                                                                          cGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAg                                                       <  1:1508405/148‑1 (MQ=255)
                                                                                           ggTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGa                                                      <  2:2147519/148‑1 (MQ=255)
                                                                                            gTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGAc                                                     <  2:2910089/148‑1 (MQ=255)
                                                                                              gCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAg                                                   <  2:2179540/148‑1 (MQ=255)
                                                                                              gCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAg                                                   <  2:2179568/148‑1 (MQ=255)
                                                                                              gCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAg                                                   <  1:1707629/148‑1 (MQ=255)
                                                                                               ccAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGc                                                  <  2:262148/148‑1 (MQ=255)
                                                                                                   aaCCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCa                                              >  2:2090812/1‑148 (MQ=255)
                                                                                                       tGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATTATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGa                                          <  1:2610774/148‑1 (MQ=255)
                                                                                                       tGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGa                                          >  2:396942/1‑148 (MQ=255)
                                                                                                        gCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGat                                         >  1:1791870/1‑148 (MQ=255)
                                                                                                         cccGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGata                                        <  2:1939207/148‑1 (MQ=255)
                                                                                                          ccGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGatat                                       <  2:3047354/148‑1 (MQ=255)
                                                                                                           cGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATg                                      <  2:3448430/148‑1 (MQ=255)
                                                                                                            gTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATgg                                     >  1:3116092/1‑148 (MQ=255)
                                                                                                             tGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGt                                    >  1:57834/1‑148 (MQ=255)
                                                                                                              gCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTg                                   >  2:1066793/1‑148 (MQ=255)
                                                                                                               cGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGc                                  <  1:3412163/148‑1 (MQ=255)
                                                                                                                 aaGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtg                                >  1:298150/1‑148 (MQ=255)
                                                                                                                  aGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtgg                               >  1:979194/1‑148 (MQ=255)
                                                                                                                    tGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtggtg                             <  2:2484442/148‑1 (MQ=255)
                                                                                                                    tGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtggtg                             <  2:1770566/148‑1 (MQ=255)
                                                                                                                        ttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTc                         >  2:3082655/1‑148 (MQ=255)
                                                                                                                         tGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTct                        <  1:3293421/148‑1 (MQ=255)
                                                                                                                         tGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTct                        <  1:2839386/148‑1 (MQ=255)
                                                                                                                            ttttAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGAGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctgct                     >  2:3057479/1‑148 (MQ=255)
                                                                                                                             tttAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctgctg                    <  2:481138/148‑1 (MQ=255)
                                                                                                                              ttAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCtgctgc                   >  2:566144/1‑148 (MQ=255)
                                                                                                                                 aaTCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTg                >  1:1183522/1‑148 (MQ=255)
                                                                                                                                 aaTCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTg                >  1:2029598/1‑148 (MQ=255)
                                                                                                                                  aTCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTgg               >  2:2855790/1‑148 (MQ=255)
                                                                                                                                    cATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCt             >  2:67894/1‑148 (MQ=255)
                                                                                                                                       atGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGGGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAt          >  1:2313092/1‑148 (MQ=255)
                                                                                                                                       atGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAt          <  1:2713552/148‑1 (MQ=255)
                                                                                                                                       atGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAt          >  1:3067725/1‑148 (MQ=255)
                                                                                                                                           ttAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAtttat      >  2:188439/1‑148 (MQ=255)
                                                                                                                                            tAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAtttatg     >  2:794215/1‑148 (MQ=255)
                                                                                                                                             agatATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAtttatgt    >  2:12202/1‑148 (MQ=255)
                                                                                                                                               agaTATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAtttatgttt  <  2:1013608/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
ATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTT  >  CP009273/137771‑138061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA  >  CP009273/137766‑138062
                                                                                                                                                     |                                                                                                                                                   
TTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAT                                                                                                                                                     <  SRR19973067.1561583/150‑1 (MQ=60)
TTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAT                                                                                                                                                     <  SRR19973067.1561582/150‑1 (MQ=60)
TTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAT                                                                                                                                                     <  SRR19973067.1561490/150‑1 (MQ=60)
TTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAT                                                                                                                                                     >  SRR19973067.286410/1‑150 (MQ=60)
TTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAT                                                                                                                                                     <  SRR19973067.1449259/150‑1 (MQ=60)
 TAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATA                                                                                                                                                    >  SRR19973067.1644912/1‑150 (MQ=60)
   ACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATA                                                                                                                                                  >  SRR19973067.2263916/1‑150 (MQ=60)
   ACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATA                                                                                                                                                  <  SRR19973067.3101910/150‑1 (MQ=60)
     ATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATG                                                                                                                                                >  SRR19973067.888560/1‑150 (MQ=60)
     ATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATG                                                                                                                                                >  SRR19973067.1127517/1‑150 (MQ=60)
     ATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATG                                                                                                                                                <  SRR19973067.1364906/150‑1 (MQ=60)
       GTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAA                                                                                                                                              >  SRR19973067.1454830/1‑150 (MQ=60)
       GTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAA                                                                                                                                              >  SRR19973067.725756/1‑150 (MQ=60)
         GTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAAC                                                                                                                                            >  SRR19973067.372026/1‑150 (MQ=60)
           GATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACAT                                                                                                                                          <  SRR19973067.3118463/150‑1 (MQ=60)
              CGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACT                                                                                                                                       >  SRR19973067.1764427/1‑150 (MQ=60)
               GTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTG                                                                                                                                      <  SRR19973067.1380847/150‑1 (MQ=60)
                TCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGT                                                                                                                                     >  SRR19973067.666359/1‑150 (MQ=60)
                 CATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTA                                                                                                                                    >  SRR19973067.2513878/1‑150 (MQ=60)
                  ATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAG                                                                                                                                   <  SRR19973067.1052405/150‑1 (MQ=60)
                  ATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAG                                                                                                                                   <  SRR19973067.2248860/150‑1 (MQ=60)
                  ATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAG                                                                                                                                   <  SRR19973067.2248864/150‑1 (MQ=60)
                  ATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAG                                                                                                                                   >  SRR19973067.496651/1‑150 (MQ=60)
                  ATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAG                                                                                                                                   <  SRR19973067.1052614/150‑1 (MQ=60)
                   TCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGA                                                                                                                                  >  SRR19973067.206419/1‑150 (MQ=60)
                   TCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGAGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATTAAACATACTGTAGA                                                                                                                                  >  SRR19973067.2013790/1‑150 (MQ=60)
                       AATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTA                                                                                                                              >  SRR19973067.2928630/1‑150 (MQ=60)
                        ATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAA                                                                                                                             >  SRR19973067.191321/1‑150 (MQ=60)
                         TTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAAT                                                                                                                            <  SRR19973067.3180734/150‑1 (MQ=60)
                          TCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATG                                                                                                                           <  SRR19973067.1634549/150‑1 (MQ=60)
                             AGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATC                                                                                                                        >  SRR19973067.6651/1‑150 (MQ=60)
                              GCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCC                                                                                                                       >  SRR19973067.2641002/1‑150 (MQ=60)
                               CTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCC                                                                                                                      <  SRR19973067.926936/150‑1 (MQ=60)
                                   ATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAA                                                                                                                  >  SRR19973067.3101653/1‑150 (MQ=60)
                                     TAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGC                                                                                                                >  SRR19973067.798292/1‑150 (MQ=60)
                                     TAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGC                                                                                                                >  SRR19973067.945616/1‑150 (MQ=60)
                                         AGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAG                                                                                                            >  SRR19973067.2607599/1‑150 (MQ=60)
                                            TTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATT                                                                                                         >  SRR19973067.1901315/1‑150 (MQ=60)
                                                   AATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGC                                                                                                  >  SRR19973067.2945675/1‑150 (MQ=60)
                                                   AATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGC                                                                                                  >  SRR19973067.2946822/1‑150 (MQ=60)
                                                           GTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCC                                                                                          >  SRR19973067.648611/1‑150 (MQ=60)
                                                           GTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCC                                                                                          <  SRR19973067.160173/150‑1 (MQ=60)
                                                           GTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCC                                                                                          <  SRR19973067.160662/150‑1 (MQ=60)
                                                           GTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCC                                                                                          >  SRR19973067.2445200/1‑150 (MQ=60)
                                                                ACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACT                                                                                     >  SRR19973067.1956970/1‑150 (MQ=60)
                                                                    GTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGT                                                                                 <  SRR19973067.1420377/150‑1 (MQ=60)
                                                                    GTATACTGCGTGTCTTGCGCTTTGGTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGT                                                                                 <  SRR19973067.1419841/150‑1 (MQ=60)
                                                                     TATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTC                                                                                <  SRR19973067.1306173/150‑1 (MQ=60)
                                                                     TATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTC                                                                                >  SRR19973067.834052/1‑150 (MQ=60)
                                                                          TGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAG                                                                           <  SRR19973067.1111500/150‑1 (MQ=60)
                                                                               GTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTAC                                                                      >  SRR19973067.2289818/1‑150 (MQ=60)
                                                                               GTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTAC                                                                      >  SRR19973067.1540821/1‑150 (MQ=60)
                                                                                  TTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGA                                                                   >  SRR19973067.2926919/1‑150 (MQ=60)
                                                                                   TGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAG                                                                  >  SRR19973067.3267772/1‑150 (MQ=60)
                                                                                    GCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGC                                                                 >  SRR19973067.1614775/1‑150 (MQ=60)
                                                                                           GTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAA                                                          >  SRR19973067.2775879/1‑150 (MQ=60)
                                                                                            TTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAA                                                         >  SRR19973067.152620/1‑150 (MQ=60)
                                                                                              GCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGA                                                       <  SRR19973067.1651268/150‑1 (MQ=60)
                                                                                               CGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGAC                                                      <  SRR19973067.573244/150‑1 (MQ=60)
                                                                                               CGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGAC                                                      <  SRR19973067.1518401/150‑1 (MQ=60)
                                                                                                GGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACA                                                     <  SRR19973067.1066831/150‑1 (MQ=60)
                                                                                                   GCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCG                                                  <  SRR19973067.1719341/150‑1 (MQ=60)
                                                                                                     CAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGC                                                >  SRR19973067.1491577/1‑150 (MQ=60)
                                                                                                           CTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGAT                                          >  SRR19973067.1803943/1‑150 (MQ=60)
                                                                                                           CTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGAT                                          >  SRR19973067.1803919/1‑150 (MQ=60)
                                                                                                            TGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATTATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATA                                         <  SRR19973067.2627727/150‑1 (MQ=60)
                                                                                                               CCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGG                                      >  SRR19973067.3135903/1‑150 (MQ=60)
                                                                                                               CCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGG                                      >  SRR19973067.1693177/1‑150 (MQ=60)
                                                                                                                CGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGT                                     >  SRR19973067.58551/1‑150 (MQ=60)
                                                                                                                CGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGT                                     >  SRR19973067.58558/1‑150 (MQ=60)
                                                                                                                 GTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTG                                    >  SRR19973067.1448436/1‑150 (MQ=60)
                                                                                                                    CGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTG                                 <  SRR19973067.3433883/150‑1 (MQ=60)
                                                                                                                    CGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTG                                 >  SRR19973067.300047/1‑150 (MQ=60)
                                                                                                                     GAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGG                                >  SRR19973067.985916/1‑150 (MQ=60)
                                                                                                                              TGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGC                       <  SRR19973067.3314136/150‑1 (MQ=60)
                                                                                                                              TGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGC                       <  SRR19973067.2857617/150‑1 (MQ=60)
                                                                                                                                    TAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTG                 >  SRR19973067.2043260/1‑150 (MQ=60)
                                                                                                                                    TAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTG                 >  SRR19973067.2043210/1‑150 (MQ=60)
                                                                                                                                    TAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTG                 >  SRR19973067.1191323/1‑150 (MQ=60)
                                                                                                                                          ATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGGGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAT           >  SRR19973067.2328319/1‑150 (MQ=60)
                                                                                                                                          ATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAT           <  SRR19973067.3154432/150‑1 (MQ=60)
                                                                                                                                          ATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAT           >  SRR19973067.3087249/1‑150 (MQ=60)
                                                                                                                                            ATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTT         <  SRR19973067.2731041/150‑1 (MQ=60)
                                                                                                                                                 TAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTT    <  SRR19973067.3004808/150‑1 (MQ=60)
                                                                                                                                                   GATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA  <  SRR19973067.750886/150‑1 (MQ=60)
                                                                                                                                                   GATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA  <  SRR19973067.750904/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTA  >  CP009273/137766‑138062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑