Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 137,915 G→T 100% intergenic (‑203/‑3) gcd ← / → hpt glucose dehydrogenase/hypoxanthine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273137,9150GT99.5% 698.0 / ‑4.3 212intergenic (‑203/‑3)gcd/hptglucose dehydrogenase/hypoxanthine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (113/98);  total (113/99)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

CATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGT  >  CP009273/137770‑138059
                                                                                                                                                 |                                                                                                                                                
cATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGAGCGAAGTGATTTGTTTTTAAATCATATGGTTAGatat                                                                                                                                                <  2:3324610/148‑1 (MQ=255)
 aTGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAtata                                                                                                                                               <  1:1018284/148‑1 (MQ=255)
         tCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACAt                                                                                                                                       <  2:1132934/148‑1 (MQ=255)
         tCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACAt                                                                                                                                       >  2:2057344/1‑148 (MQ=255)
          cGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATa                                                                                                                                      >  2:3557071/1‑148 (MQ=255)
            tcatcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACt                                                                                                                                    >  1:2894902/1‑148 (MQ=255)
             catcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTg                                                                                                                                   >  2:1055230/1‑148 (MQ=255)
             catcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTg                                                                                                                                   <  1:2972977/148‑1 (MQ=255)
                cacaATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAg                                                                                                                                >  2:3069139/1‑148 (MQ=255)
                cacaATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAg                                                                                                                                >  2:2923940/1‑148 (MQ=255)
                 acaATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGa                                                                                                                               <  1:2381126/148‑1 (MQ=255)
                  caATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGaa                                                                                                                              <  1:1111916/148‑1 (MQ=255)
                   aaTTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAg                                                                                                                             >  1:1214387/1‑148 (MQ=255)
                     ttCTAGCTTTATTAAGATATTGCTCGTATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTa                                                                                                                           <  2:2944976/148‑1 (MQ=255)
                     ttCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTa                                                                                                                           <  2:1599020/148‑1 (MQ=255)
                      tCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTaa                                                                                                                          <  2:3741417/148‑1 (MQ=255)
                      tCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTAATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTaa                                                                                                                          <  1:3129033/148‑1 (MQ=255)
                       cGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAAt                                                                                                                         <  1:1760247/148‑1 (MQ=255)
                         aGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGa                                                                                                                       <  2:3120953/148‑1 (MQ=255)
                           cTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATc                                                                                                                     >  1:1692896/1‑148 (MQ=255)
                           cTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCAGCCCGTGCGAAGTGATTTGTTTTTAAATCATCTGGTTAGATATATGAAACATACTGTAGGAGTAATGATc                                                                                                                     >  2:620985/1‑148 (MQ=255)
                            tttATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATcc                                                                                                                    <  2:2422137/148‑1 (MQ=255)
                                ttaACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGa                                                                                                                <  2:1776936/148‑1 (MQ=255)
                                ttaACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGa                                                                                                                <  2:416564/148‑1 (MQ=255)
                                 taACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGaa                                                                                                               >  2:842230/1‑148 (MQ=255)
                                 taACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGaa                                                                                                               >  2:1441442/1‑148 (MQ=255)
                                 taACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGaa                                                                                                               >  2:684734/1‑148 (MQ=255)
                                 taACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGaa                                                                                                               <  1:1990788/148‑1 (MQ=255)
                                        ttCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGgaga                                                                                                        <  2:2448817/148‑1 (MQ=255)
                                         tCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGAt                                                                                                       >  2:790937/1‑148 (MQ=255)
                                         tCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGAt                                                                                                       >  2:726272/1‑148 (MQ=255)
                                             cgAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTaaa                                                                                                   <  2:2027570/148‑1 (MQ=255)
                                             cgAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTaaa                                                                                                   <  2:719516/148‑1 (MQ=255)
                                               aatgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgc                                                                                                 >  1:736301/1‑148 (MQ=255)
                                                atgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgcg                                                                                                <  2:64720/148‑1 (MQ=255)
                                                atgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgcg                                                                                                >  1:1340235/1‑148 (MQ=255)
                                                atgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgcg                                                                                                <  1:402908/148‑1 (MQ=255)
                                                atgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgcg                                                                                                >  1:140445/1‑148 (MQ=255)
                                                  gaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGcgcg                                                                                              <  2:1237269/148‑1 (MQ=255)
                                                     tAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTAt                                                                                           >  1:2547106/1‑148 (MQ=255)
                                                       gTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCg                                                                                         <  2:175650/148‑1 (MQ=255)
                                                        ttttACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGc                                                                                        >  1:2205300/1‑148 (MQ=255)
                                                         tttACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGcc                                                                                       >  1:1847870/1‑148 (MQ=255)
                                                         tttACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGcc                                                                                       <  2:1821797/148‑1 (MQ=255)
                                                            aCTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCTAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGaa                                                                                    <  2:2502674/148‑1 (MQ=255)
                                                            aCTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCTAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGaa                                                                                    <  2:1873477/148‑1 (MQ=255)
                                                                    aCTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgtcg                                                                            >  2:2178266/1‑148 (MQ=255)
                                                                    aCTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgtcg                                                                            >  2:3667990/1‑148 (MQ=255)
                                                                     cTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgtcgt                                                                           <  1:1469626/148‑1 (MQ=255)
                                                                        cGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAg                                                                        >  2:3105832/1‑148 (MQ=255)
                                                                        cGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAg                                                                        <  1:357241/148‑1 (MQ=255)
                                                                          tgtCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGAt                                                                      <  1:775600/148‑1 (MQ=255)
                                                                            tCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTa                                                                    <  2:3395663/148‑1 (MQ=255)
                                                                                   cTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCg                                                             <  2:1173175/148‑1 (MQ=255)
                                                                                   cTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCg                                                             <  2:2325223/148‑1 (MQ=255)
                                                                                     ttgttgCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGtt                                                           >  1:3358636/1‑148 (MQ=255)
                                                                                     ttgttgCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGtt                                                           >  1:3191141/1‑148 (MQ=255)
                                                                                     ttgttgCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGtt                                                           <  2:504090/148‑1 (MQ=255)
                                                                                        ttgCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACa                                                        <  1:110800/148‑1 (MQ=255)
                                                                                              tGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACa                                                  <  1:2535959/148‑1 (MQ=255)
                                                                                               gCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAg                                                 >  1:46735/1‑148 (MQ=255)
                                                                                                   aaaCCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGc                                             >  2:1650090/1‑148 (MQ=255)
                                                                                                   aaaCCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGc                                             >  2:1313176/1‑148 (MQ=255)
                                                                                                   aaaCCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGc                                             <  2:2198494/148‑1 (MQ=255)
                                                                                                     aCCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAg                                           >  2:857784/1‑148 (MQ=255)
                                                                                                      ccTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATCCTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGc                                          >  2:276824/1‑148 (MQ=255)
                                                                                                      ccTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGc                                          >  1:2624667/1‑148 (MQ=255)
                                                                                                        tGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGa                                        >  1:538516/1‑148 (MQ=255)
                                                                                                         gCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGat                                       <  1:2057344/148‑1 (MQ=255)
                                                                                                         gCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGat                                       <  2:3505325/148‑1 (MQ=255)
                                                                                                         gCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGat                                       >  1:435737/1‑148 (MQ=255)
                                                                                                          cccGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGata                                      <  1:1332780/148‑1 (MQ=255)
                                                                                                          cccGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGata                                      >  2:826339/1‑148 (MQ=255)
                                                                                                             gTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATgg                                   >  1:2314596/1‑148 (MQ=255)
                                                                                                               gCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTg                                 <  2:3366555/148‑1 (MQ=255)
                                                                                                                    gTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtggt                            >  1:1872730/1‑148 (MQ=255)
                                                                                                                     tGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtggtg                           >  1:2519031/1‑148 (MQ=255)
                                                                                                                       aTTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTggg                         >  1:1308456/1‑148 (MQ=255)
                                                                                                                        tttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGt                        <  1:3313092/148‑1 (MQ=255)
                                                                                                                        tttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGt                        <  2:140445/148‑1 (MQ=255)
                                                                                                                         ttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTc                       >  2:2971295/1‑148 (MQ=255)
                                                                                                                         ttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTc                       >  2:575521/1‑148 (MQ=255)
                                                                                                                         ttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTc                       <  2:2538069/148‑1 (MQ=255)
                                                                                                                         ttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGGTACAAAGACAGCGGCAGCGATATGTTGCTGGTGGGTc                       >  1:2411870/1‑148 (MQ=255)
                                                                                                                          tGTTTTTAAATCATATTGATAGATATATTAAACATACTGTAGAAGTAAATATCCCCGAAGAGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGAct                      >  2:2224850/1‑148 (MQ=255)
                                                                                                                           gTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctg                     <  1:2724868/148‑1 (MQ=255)
                                                                                                                             ttttAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctgct                   >  1:1599532/1‑148 (MQ=255)
                                                                                                                             ttttAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctgct                   >  2:1577194/1‑148 (MQ=255)
                                                                                                                                tAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCg                <  1:2091822/148‑1 (MQ=255)
                                                                                                                                 aaaTCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGCTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGt               <  2:1592669/148‑1 (MQ=255)
                                                                                                                                   aTCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTgg             >  1:478730/1‑148 (MQ=255)
                                                                                                                                    tCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGc            <  1:3702981/148‑1 (MQ=255)
                                                                                                                                     cATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCt           >  2:2672935/1‑148 (MQ=255)
                                                                                                                                      atatGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTc          >  2:1563640/1‑148 (MQ=255)
                                                                                                                                         tGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAtt       >  2:594551/1‑148 (MQ=255)
                                                                                                                                          ggTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAttt      <  1:1167963/148‑1 (MQ=255)
                                                                                                                                          ggTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAttt      <  1:2923940/148‑1 (MQ=255)
                                                                                                                                          ggTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAttt      <  2:2334546/148‑1 (MQ=255)
                                                                                                                                             tAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAtttatg   <  1:2601426/148‑1 (MQ=255)
                                                                                                                                              agatATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAtttatgt  <  2:1340235/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGT  >  CP009273/137770‑138059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTAT  >  CP009273/137767‑138063
                                                                                                                                                    |                                                                                                                                                    
TAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATA                                                                                                                                                     >  SRR19973069.973704/1‑150 (MQ=60)
   CATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATAT                                                                                                                                                  <  SRR19973069.1631678/150‑1 (MQ=60)
    ATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATG                                                                                                                                                 <  SRR19973069.1025320/150‑1 (MQ=60)
    ATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATG                                                                                                                                                 <  SRR19973069.2946665/150‑1 (MQ=60)
        GTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAAC                                                                                                                                             >  SRR19973069.2554877/1‑150 (MQ=60)
           ATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATA                                                                                                                                          >  SRR19973069.1866140/1‑150 (MQ=60)
             CGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACT                                                                                                                                        >  SRR19973069.2913997/1‑150 (MQ=60)
                CATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTA                                                                                                                                     <  SRR19973069.2992716/150‑1 (MQ=60)
                 ATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAG                                                                                                                                    <  SRR19973069.2731914/150‑1 (MQ=60)
                    ACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAG                                                                                                                                 >  SRR19973069.1041449/1‑150 (MQ=60)
                    ACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAG                                                                                                                                 >  SRR19973069.2585594/1‑150 (MQ=60)
                    ACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAG                                                                                                                                 <  SRR19973069.2397274/150‑1 (MQ=60)
                    ACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAG                                                                                                                                 <  SRR19973069.1406475/150‑1 (MQ=60)
                    ACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAG                                                                                                                                 >  SRR19973069.1222711/1‑150 (MQ=60)
                     CAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGT                                                                                                                                <  SRR19973069.1119677/150‑1 (MQ=60)
                     CAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGT                                                                                                                                <  SRR19973069.2690414/150‑1 (MQ=60)
                     CAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGT                                                                                                                                <  SRR19973069.1119674/150‑1 (MQ=60)
                        TTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAAT                                                                                                                             <  SRR19973069.2024496/150‑1 (MQ=60)
                         TCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTAATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATG                                                                                                                            <  SRR19973069.3149559/150‑1 (MQ=60)
                          CGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGA                                                                                                                           <  SRR19973069.1772658/150‑1 (MQ=60)
                            AGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATC                                                                                                                         >  SRR19973069.1704721/1‑150 (MQ=60)
                                 TATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGA                                                                                                                    >  SRR19973069.3047958/1‑150 (MQ=60)
                                    TAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGC                                                                                                                 <  SRR19973069.2004668/150‑1 (MQ=60)
                                     AACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCG                                                                                                                <  SRR19973069.1793572/150‑1 (MQ=60)
                                                CGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGC                                                                                                     >  SRR19973069.741518/1‑150 (MQ=60)
                                                 GAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCG                                                                                                    >  SRR19973069.3342745/1‑150 (MQ=60)
                                                 GAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCG                                                                                                    >  SRR19973069.1296156/1‑150 (MQ=60)
                                                 GAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCG                                                                                                    >  SRR19973069.1296170/1‑150 (MQ=60)
                                                 GAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCG                                                                                                    >  SRR19973069.1349493/1‑150 (MQ=60)
                                                 GAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCG                                                                                                    >  SRR19973069.141728/1‑150 (MQ=60)
                                                   ATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCG                                                                                                  <  SRR19973069.405863/150‑1 (MQ=60)
                                                      AATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTAT                                                                                               >  SRR19973069.2348547/1‑150 (MQ=60)
                                                      AATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTAT                                                                                               >  SRR19973069.2563945/1‑150 (MQ=60)
                                                         AGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGC                                                                                            >  SRR19973069.2220260/1‑150 (MQ=60)
                                                         AGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGC                                                                                            >  SRR19973069.2220214/1‑150 (MQ=60)
                                                          GTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCC                                                                                           >  SRR19973069.1863367/1‑150 (MQ=60)
                                                          GTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCC                                                                                           >  SRR19973069.1346461/1‑150 (MQ=60)
                                                          GTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCC                                                                                           >  SRR19973069.1860781/1‑150 (MQ=60)
                                                             TTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAA                                                                                        <  SRR19973069.1886669/150‑1 (MQ=60)
                                                                CTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTG                                                                                     <  SRR19973069.3089418/150‑1 (MQ=60)
                                                                 TGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGG                                                                                    <  SRR19973069.796553/150‑1 (MQ=60)
                                                                        CTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCA                                                                             <  SRR19973069.697014/150‑1 (MQ=60)
                                                                        CTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCA                                                                             <  SRR19973069.1479870/150‑1 (MQ=60)
                                                                           CGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGAT                                                                          <  SRR19973069.359801/150‑1 (MQ=60)
                                                                             TGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTA                                                                        <  SRR19973069.781100/150‑1 (MQ=60)
                                                                             TGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTA                                                                        <  SRR19973069.2509463/150‑1 (MQ=60)
                                                                                  TGCGCCTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAG                                                                   <  SRR19973069.2159884/150‑1 (MQ=60)
                                                                                      CTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTT                                                               >  SRR19973069.3211860/1‑150 (MQ=60)
                                                                                      CTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTT                                                               >  SRR19973069.3380614/1‑150 (MQ=60)
                                                                                         TGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACA                                                            <  SRR19973069.3184692/150‑1 (MQ=60)
                                                                                           TTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAA                                                          <  SRR19973069.111888/150‑1 (MQ=60)
                                                                                           TTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAA                                                          <  SRR19973069.112270/150‑1 (MQ=60)
                                                                                               GGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACA                                                      <  SRR19973069.771185/150‑1 (MQ=60)
                                                                                                GTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAG                                                     >  SRR19973069.1332507/1‑150 (MQ=60)
                                                                                                GTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAG                                                     <  SRR19973069.3557093/150‑1 (MQ=60)
                                                                                                GTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAG                                                     >  SRR19973069.47304/1‑150 (MQ=60)
                                                                                                GTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAG                                                     >  SRR19973069.47327/1‑150 (MQ=60)
                                                                                                 TGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGC                                                    <  SRR19973069.2552759/150‑1 (MQ=60)
                                                                                                 TGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGC                                                    >  SRR19973069.2904852/1‑150 (MQ=60)
                                                                                                   CCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGG                                                  >  SRR19973069.144328/1‑150 (MQ=60)
                                                                                                       AACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGC                                              >  SRR19973069.2641476/1‑150 (MQ=60)
                                                                                                       AACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGC                                              >  SRR19973069.2642228/1‑150 (MQ=60)
                                                                                                         CCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGA                                            >  SRR19973069.542313/1‑150 (MQ=60)
                                                                                                          CTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGAT                                           >  SRR19973069.438805/1‑150 (MQ=60)
                                                                                                          CTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGAT                                           >  SRR19973069.438932/1‑150 (MQ=60)
                                                                                                          CTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGAT                                           >  SRR19973069.471298/1‑150 (MQ=60)
                                                                                                            GCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATAT                                         <  SRR19973069.2071617/150‑1 (MQ=60)
                                                                                                             CCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATG                                        <  SRR19973069.1341966/150‑1 (MQ=60)
                                                                                                              CCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGG                                       >  SRR19973069.2330348/1‑150 (MQ=60)
                                                                                                                     AAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGT                                >  SRR19973069.1885763/1‑150 (MQ=60)
                                                                                                                      AGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTG                               >  SRR19973069.1453433/1‑150 (MQ=60)
                                                                                                                      AGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTG                               >  SRR19973069.3237948/1‑150 (MQ=60)
                                                                                                                      AGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTG                               >  SRR19973069.2535784/1‑150 (MQ=60)
                                                                                                                        TGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGG                             >  SRR19973069.1317393/1‑150 (MQ=60)
                                                                                                                        TGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGG                             >  SRR19973069.2080881/1‑150 (MQ=60)
                                                                                                                          ATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTC                           >  SRR19973069.2428452/1‑150 (MQ=60)
                                                                                                                          ATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGGTACAAAGACAGCGGCAGCGATATGTTGCTGGTGGGTC                           >  SRR19973069.2428172/1‑150 (MQ=60)
                                                                                                                           TTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCT                          <  SRR19973069.157468/150‑1 (MQ=60)
                                                                                                                           TTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCT                          <  SRR19973069.1729957/150‑1 (MQ=60)
                                                                                                                           TTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCT                          <  SRR19973069.3334807/150‑1 (MQ=60)
                                                                                                                           TTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCT                          <  SRR19973069.539953/150‑1 (MQ=60)
                                                                                                                              GTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCT                       <  SRR19973069.2743123/150‑1 (MQ=60)
                                                                                                                              GTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCT                       >  SRR19973069.1610318/1‑150 (MQ=60)
                                                                                                                                  TTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGT                   >  SRR19973069.2924839/1‑150 (MQ=60)
                                                                                                                                   TAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTG                  >  SRR19973069.3767699/1‑150 (MQ=60)
                                                                                                                                   TAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTG                  <  SRR19973069.2106287/150‑1 (MQ=60)
                                                                                                                                    AAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGG                 >  SRR19973069.343304/1‑150 (MQ=60)
                                                                                                                                    AAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGG                 >  SRR19973069.482206/1‑150 (MQ=60)
                                                                                                                                    AAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGG                 >  SRR19973069.3637655/1‑150 (MQ=60)
                                                                                                                                       TCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTC              <  SRR19973069.3727181/150‑1 (MQ=60)
                                                                                                                                         ATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAATCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGAGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAT            >  SRR19973069.2222319/1‑150 (MQ=60)
                                                                                                                                         ATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAT            >  SRR19973069.2405196/1‑150 (MQ=60)
                                                                                                                                         ATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAT            >  SRR19973069.471032/1‑150 (MQ=60)
                                                                                                                                             GGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTAT        >  SRR19973069.1197130/1‑150 (MQ=60)
                                                                                                                                             GGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTAT        <  SRR19973069.1176071/150‑1 (MQ=60)
                                                                                                                                             GGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTAT        <  SRR19973069.2943312/150‑1 (MQ=60)
                                                                                                                                                TAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTT     <  SRR19973069.2618794/150‑1 (MQ=60)
                                                                                                                                                 AGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTT    <  SRR19973069.2547917/150‑1 (MQ=60)
                                                                                                                                                   ATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTAT  >  SRR19973069.2087612/1‑150 (MQ=60)
                                                                                                                                                   ATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTAT  >  SRR19973069.1616936/1‑150 (MQ=60)
                                                                                                                                                   ATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTAT  >  SRR19973069.1177317/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTAT  >  CP009273/137767‑138063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑