Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 137,915 G→T 100% intergenic (‑203/‑3) gcd ← / → hpt glucose dehydrogenase/hypoxanthine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273137,9150GT99.0% 643.4 / ‑1.8 200intergenic (‑203/‑3)gcd/hptglucose dehydrogenase/hypoxanthine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (94/102);  total (94/106)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

CATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATG  >  CP009273/137770‑138058
                                                                                                                                                 |                                                                                                                                               
cATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGatat                                                                                                                                               <  2:588392/148‑1 (MQ=255)
 aTGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAtata                                                                                                                                              <  1:571577/148‑1 (MQ=255)
 aTGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAtata                                                                                                                                              <  1:2338382/148‑1 (MQ=255)
     gtgATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGaa                                                                                                                                          >  1:1085870/1‑148 (MQ=255)
     gtgATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGaa                                                                                                                                          <  1:717335/148‑1 (MQ=255)
     gtgATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGaa                                                                                                                                          <  1:1799053/148‑1 (MQ=255)
      tgATCGTCATCACAATTCGAGCTTTATTAACAGAGTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGaaa                                                                                                                                         <  2:2347603/148‑1 (MQ=255)
         tCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACAt                                                                                                                                      >  1:2911909/1‑148 (MQ=255)
         tCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGGGATTTGTTTTTAAATCATATGGTTAGATATATGAAACAt                                                                                                                                      >  2:1925591/1‑148 (MQ=255)
          cGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATa                                                                                                                                     <  1:3236506/148‑1 (MQ=255)
          cGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATa                                                                                                                                     >  1:287578/1‑148 (MQ=255)
             catcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATTCTTAGATATATGAAACATACTg                                                                                                                                  >  2:1823105/1‑148 (MQ=255)
             catcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTg                                                                                                                                  >  1:313369/1‑148 (MQ=255)
             catcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTg                                                                                                                                  >  1:317086/1‑148 (MQ=255)
              atcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGt                                                                                                                                 >  1:668281/1‑148 (MQ=255)
               tcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTa                                                                                                                                >  2:2163618/1‑148 (MQ=255)
                 acaATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGa                                                                                                                              >  1:2141637/1‑148 (MQ=255)
                 acaATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGa                                                                                                                              >  2:538243/1‑148 (MQ=255)
                  caATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGaa                                                                                                                             <  2:276714/148‑1 (MQ=255)
                  caATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGaa                                                                                                                             <  2:853702/148‑1 (MQ=255)
                      tCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTaa                                                                                                                         >  2:1110300/1‑148 (MQ=255)
                      tCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTaa                                                                                                                         >  1:2595917/1‑148 (MQ=255)
                      tCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTaa                                                                                                                         >  2:727429/1‑148 (MQ=255)
                         aGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGa                                                                                                                      <  1:2160369/148‑1 (MQ=255)
                          gCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGAt                                                                                                                     >  2:1119388/1‑148 (MQ=255)
                          gCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGAt                                                                                                                     >  1:3050382/1‑148 (MQ=255)
                          gCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGAt                                                                                                                     >  2:2634992/1‑148 (MQ=255)
                           cTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATc                                                                                                                    >  1:343573/1‑148 (MQ=255)
                            tttATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATcc                                                                                                                   <  1:2716748/148‑1 (MQ=255)
                              tattaACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATcccc                                                                                                                 >  1:964034/1‑148 (MQ=255)
                                ttaACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGa                                                                                                               >  1:3276106/1‑148 (MQ=255)
                                 taACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGaa                                                                                                              >  2:1260807/1‑148 (MQ=255)
                                   aCAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGACATATGAAACATACTGTAGAAGTAATGATCCCCGAAGc                                                                                                            <  1:1822091/148‑1 (MQ=255)
                                     aGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCgg                                                                                                          >  1:1944721/1‑148 (MQ=255)
                                     aGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCgg                                                                                                          <  2:38169/148‑1 (MQ=255)
                                         tCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGAt                                                                                                      <  2:2596462/148‑1 (MQ=255)
                                               aatgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgc                                                                                                >  2:1705510/1‑148 (MQ=255)
                                               aatgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgc                                                                                                >  1:1322272/1‑148 (MQ=255)
                                                atgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgcg                                                                                               <  2:1354436/148‑1 (MQ=255)
                                                 tgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgcgc                                                                                              <  2:2431032/148‑1 (MQ=255)
                                                   aatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGt                                                                                            >  1:1501008/1‑148 (MQ=255)
                                                      aGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATc                                                                                         >  2:2708680/1‑148 (MQ=255)
                                                         tttACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGcc                                                                                      >  2:2758728/1‑148 (MQ=255)
                                                             cTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAAc                                                                                  <  1:2946799/148‑1 (MQ=255)
                                                                gTATCCTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTgg                                                                               <  2:1281067/148‑1 (MQ=255)
                                                                gTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTgg                                                                               >  1:1254125/1‑148 (MQ=255)
                                                                gTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTgg                                                                               <  2:1280948/148‑1 (MQ=255)
                                                                gTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTgg                                                                               <  2:1184560/148‑1 (MQ=255)
                                                                 tataCTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTggg                                                                              >  1:2325126/1‑148 (MQ=255)
                                                                  ataCTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgt                                                                             <  2:2139704/148‑1 (MQ=255)
                                                                   taCTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgtc                                                                            >  1:3221848/1‑148 (MQ=255)
                                                                    aCTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgtcg                                                                           >  2:2878048/1‑148 (MQ=255)
                                                                       gCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCa                                                                        >  1:2533029/1‑148 (MQ=255)
                                                                       gCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCa                                                                        >  2:1484905/1‑148 (MQ=255)
                                                                        cGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAg                                                                       <  2:182978/148‑1 (MQ=255)
                                                                          tgtCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGAt                                                                     <  2:3343832/148‑1 (MQ=255)
                                                                           gtCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGAtt                                                                    <  2:615896/148‑1 (MQ=255)
                                                                            tCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTa                                                                   <  1:2247756/148‑1 (MQ=255)
                                                                             cTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTAc                                                                  <  1:300309/148‑1 (MQ=255)
                                                                                    tttGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGt                                                           <  1:2668625/148‑1 (MQ=255)
                                                                                    tttGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGt                                                           >  2:1731068/1‑148 (MQ=255)
                                                                                         tgCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACaa                                                      <  1:646741/148‑1 (MQ=255)
                                                                                            ggTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGa                                                   <  2:2992104/148‑1 (MQ=255)
                                                                                             gTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGAc                                                  <  2:539550/148‑1 (MQ=255)
                                                                                               gCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAg                                                <  1:375006/148‑1 (MQ=255)
                                                                                                ccAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGc                                               <  1:1855707/148‑1 (MQ=255)
                                                                                                      ccTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGc                                         >  2:614362/1‑148 (MQ=255)
                                                                                                      ccTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGc                                         >  2:2378438/1‑148 (MQ=255)
                                                                                                        tGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGa                                       >  1:3498475/1‑148 (MQ=255)
                                                                                                         gCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGat                                      <  2:2911909/148‑1 (MQ=255)
                                                                                                         gCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGat                                      >  1:3376033/1‑148 (MQ=255)
                                                                                                         gCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGat                                      >  1:1056169/1‑148 (MQ=255)
                                                                                                            cGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATg                                   <  1:631869/148‑1 (MQ=255)
                                                                                                                cGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGc                               <  1:2916244/148‑1 (MQ=255)
                                                                                                                 gAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCt                              <  2:281322/148‑1 (MQ=255)
                                                                                                                 gAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCt                              >  2:662631/1‑148 (MQ=255)
                                                                                                                   aGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtgg                            <  2:964034/148‑1 (MQ=255)
                                                                                                                   aGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtgg                            >  1:3055600/1‑148 (MQ=255)
                                                                                                                   aGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtgg                            >  1:81253/1‑148 (MQ=255)
                                                                                                                    gTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtggt                           >  1:2023076/1‑148 (MQ=255)
                                                                                                                    gTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtggt                           <  2:2323735/148‑1 (MQ=255)
                                                                                                                     tGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtggtg                          >  1:2811754/1‑148 (MQ=255)
                                                                                                                     tGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtggtg                          >  1:1197267/1‑148 (MQ=255)
                                                                                                                       aTTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTggg                        <  1:2073663/148‑1 (MQ=255)
                                                                                                                        tttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGt                       >  1:2258049/1‑148 (MQ=255)
                                                                                                                         ttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTc                      >  2:2520329/1‑148 (MQ=255)
                                                                                                                         ttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTc                      >  1:514879/1‑148 (MQ=255)
                                                                                                                         ttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTc                      >  2:1001337/1‑148 (MQ=255)
                                                                                                                         ttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTc                      >  2:1360267/1‑148 (MQ=255)
                                                                                                                           gTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctg                    <  1:2983021/148‑1 (MQ=255)
                                                                                                                           gTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctg                    <  1:2369355/148‑1 (MQ=255)
                                                                                                                           gTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctg                    <  2:2299648/148‑1 (MQ=255)
                                                                                                                           gTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctg                    <  1:1401090/148‑1 (MQ=255)
                                                                                                                                tAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCg               <  2:1532277/148‑1 (MQ=255)
                                                                                                                                  aaTCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTg             >  1:770284/1‑148 (MQ=255)
                                                                                                                                   aTCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTgg            <  1:587029/148‑1 (MQ=255)
                                                                                                                                    tCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGc           <  2:1810846/148‑1 (MQ=255)
                                                                                                                                     cATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCt          <  1:614362/148‑1 (MQ=255)
                                                                                                                                           gTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAttta    <  2:2552616/148‑1 (MQ=255)
                                                                                                                                             tAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAtttatg  >  2:306374/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATG  >  CP009273/137770‑138058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTATG  >  CP009273/137766‑138064
                                                                                                                                                     |                                                                                                                                                     
TTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAT                                                                                                                                                       <  SRR19973065.1254333/150‑1 (MQ=60)
  AACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATAT                                                                                                                                                     <  SRR19973065.3132738/150‑1 (MQ=60)
  AACATGTGTGATAGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATAT                                                                                                                                                     <  SRR19973065.920260/150‑1 (MQ=60)
   ACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATA                                                                                                                                                    <  SRR19973065.1083775/150‑1 (MQ=60)
   ACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATA                                                                                                                                                    >  SRR19973065.404584/1‑150 (MQ=60)
     ATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATG                                                                                                                                                  <  SRR19973065.2352984/150‑1 (MQ=60)
     ATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATG                                                                                                                                                  <  SRR19973065.575186/150‑1 (MQ=60)
      TGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGA                                                                                                                                                 >  SRR19973065.368959/1‑150 (MQ=60)
       GTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAA                                                                                                                                                >  SRR19973065.1093026/1‑150 (MQ=60)
         GTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAAC                                                                                                                                              <  SRR19973065.1810865/150‑1 (MQ=60)
         GTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAAC                                                                                                                                              <  SRR19973065.722137/150‑1 (MQ=60)
         GTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAAC                                                                                                                                              <  SRR19973065.657297/150‑1 (MQ=60)
           GATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACAT                                                                                                                                            >  SRR19973065.2929631/1‑150 (MQ=60)
           GATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACAT                                                                                                                                            >  SRR19973065.2928147/1‑150 (MQ=60)
            ATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATA                                                                                                                                           <  SRR19973065.1343773/150‑1 (MQ=60)
            ATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATA                                                                                                                                           >  SRR19973065.289462/1‑150 (MQ=60)
              CGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACT                                                                                                                                         <  SRR19973065.3255987/150‑1 (MQ=60)
               GTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTG                                                                                                                                        >  SRR19973065.315486/1‑150 (MQ=60)
               GTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTG                                                                                                                                        >  SRR19973065.319258/1‑150 (MQ=60)
                TCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGT                                                                                                                                       >  SRR19973065.672751/1‑150 (MQ=60)
                TCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGT                                                                                                                                       >  SRR19973065.2696433/1‑150 (MQ=60)
                TCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGT                                                                                                                                       >  SRR19973065.672733/1‑150 (MQ=60)
                   TCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGA                                                                                                                                    >  SRR19973065.2155413/1‑150 (MQ=60)
                   TCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGA                                                                                                                                    >  SRR19973065.2153464/1‑150 (MQ=60)
                    CACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAA                                                                                                                                   >  SRR19973065.293971/1‑150 (MQ=60)
                        ATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAA                                                                                                                               >  SRR19973065.2612053/1‑150 (MQ=60)
                         TTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGTGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAAT                                                                                                                              <  SRR19973065.2326687/150‑1 (MQ=60)
                           CGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGA                                                                                                                            >  SRR19973065.1081115/1‑150 (MQ=60)
                            GAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGAT                                                                                                                           >  SRR19973065.3069044/1‑150 (MQ=60)
                            GAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGAT                                                                                                                           >  SRR19973065.3069038/1‑150 (MQ=60)
                             AGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATC                                                                                                                          <  SRR19973065.2174248/150‑1 (MQ=60)
                             AGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATC                                                                                                                          >  SRR19973065.345965/1‑150 (MQ=60)
                             AGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATC                                                                                                                          >  SRR19973065.345973/1‑150 (MQ=60)
                             AGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATC                                                                                                                          >  SRR19973065.2877447/1‑150 (MQ=60)
                             AGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATC                                                                                                                          <  SRR19973065.2174259/150‑1 (MQ=60)
                               CTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCC                                                                                                                        >  SRR19973065.3123683/1‑150 (MQ=60)
                                TTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCC                                                                                                                       <  SRR19973065.2733648/150‑1 (MQ=60)
                                TTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCC                                                                                                                       >  SRR19973065.970430/1‑150 (MQ=60)
                                  TATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGA                                                                                                                     >  SRR19973065.3296028/1‑150 (MQ=60)
                                  TATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGA                                                                                                                     >  SRR19973065.3296004/1‑150 (MQ=60)
                                    TTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAG                                                                                                                   <  SRR19973065.3471471/150‑1 (MQ=60)
                                     TAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGC                                                                                                                  <  SRR19973065.2608507/150‑1 (MQ=60)
                                       ACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGG                                                                                                                >  SRR19973065.1957434/1‑150 (MQ=60)
                                       ACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGACATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGG                                                                                                                <  SRR19973065.1834075/150‑1 (MQ=60)
                                       ACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGACATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGG                                                                                                                <  SRR19973065.1834088/150‑1 (MQ=60)
                                                 CGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGC                                                                                                      >  SRR19973065.1331015/1‑150 (MQ=60)
                                                 CGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGC                                                                                                      <  SRR19973065.864790/150‑1 (MQ=60)
                                                     TGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGT                                                                                                  >  SRR19973065.1510699/1‑150 (MQ=60)
                                                         TAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCG                                                                                              >  SRR19973065.530770/1‑150 (MQ=60)
                                                         TAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCG                                                                                              <  SRR19973065.2339937/150‑1 (MQ=60)
                                                           GTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCC                                                                                            <  SRR19973065.1205418/150‑1 (MQ=60)
                                                              TTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAA                                                                                         >  SRR19973065.3262032/1‑150 (MQ=60)
                                                                 CTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTG                                                                                      <  SRR19973065.2964729/150‑1 (MQ=60)
                                                                 CTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTG                                                                                      <  SRR19973065.2964721/150‑1 (MQ=60)
                                                                  TGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGG                                                                                     >  SRR19973065.1262382/1‑150 (MQ=60)
                                                                   GGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGG                                                                                    >  SRR19973065.2339683/1‑150 (MQ=60)
                                                                     TATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTC                                                                                  >  SRR19973065.3241207/1‑150 (MQ=60)
                                                                     TATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTC                                                                                  >  SRR19973065.3241266/1‑150 (MQ=60)
                                                                         CTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCA                                                                              >  SRR19973065.2548928/1‑150 (MQ=60)
                                                                              TGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTA                                                                         >  SRR19973065.3467217/1‑150 (MQ=60)
                                                                              TGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTA                                                                         >  SRR19973065.1719218/1‑150 (MQ=60)
                                                                                TCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACT                                                                       <  SRR19973065.2262014/150‑1 (MQ=60)
                                                                                TCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACT                                                                       <  SRR19973065.2262007/150‑1 (MQ=60)
                                                                                TCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACT                                                                       <  SRR19973065.634720/150‑1 (MQ=60)
                                                                                 CTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTG                                                                      <  SRR19973065.302244/150‑1 (MQ=60)
                                                                                 CTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTG                                                                      <  SRR19973065.302259/150‑1 (MQ=60)
                                                                                 CTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTG                                                                      <  SRR19973065.302536/150‑1 (MQ=60)
                                                                                      GCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGT                                                                 >  SRR19973065.2398406/1‑150 (MQ=60)
                                                                                        TTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTA                                                               <  SRR19973065.2685150/150‑1 (MQ=60)
                                                                                             TGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAG                                                          <  SRR19973065.650061/150‑1 (MQ=60)
                                                                                             TGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAG                                                          <  SRR19973065.237427/150‑1 (MQ=60)
                                                                                             TGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAG                                                          <  SRR19973065.650995/150‑1 (MQ=60)
                                                                                                   GCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCG                                                    <  SRR19973065.377624/150‑1 (MQ=60)
                                                                                                    CCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGG                                                   >  SRR19973065.2813939/1‑150 (MQ=60)
                                                                                                    CCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGG                                                   <  SRR19973065.1867921/150‑1 (MQ=60)
                                                                                                     CAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGC                                                  >  SRR19973065.1960637/1‑150 (MQ=60)
                                                                                                          CCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGA                                             >  SRR19973065.3519730/1‑150 (MQ=60)
                                                                                                           CTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGAT                                            >  SRR19973065.1063173/1‑150 (MQ=60)
                                                                                                           CTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGAT                                            >  SRR19973065.3396677/1‑150 (MQ=60)
                                                                                                                CGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGT                                       <  SRR19973065.635983/150‑1 (MQ=60)
                                                                                                                   GCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCT                                    <  SRR19973065.1844519/150‑1 (MQ=60)
                                                                                                                   GCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCT                                    <  SRR19973065.58009/150‑1 (MQ=60)
                                                                                                                    CGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTG                                   <  SRR19973065.2933975/150‑1 (MQ=60)
                                                                                                                    CGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTG                                   <  SRR19973065.2933982/150‑1 (MQ=60)
                                                                                                                     GAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGG                                  >  SRR19973065.3074281/1‑150 (MQ=60)
                                                                                                                     GAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGG                                  >  SRR19973065.82061/1‑150 (MQ=60)
                                                                                                                      AAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGT                                 >  SRR19973065.2036085/1‑150 (MQ=60)
                                                                                                                       AGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTG                                <  SRR19973065.1132860/150‑1 (MQ=60)
                                                                                                                       AGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTG                                >  SRR19973065.1204899/1‑150 (MQ=60)
                                                                                                                       AGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTG                                >  SRR19973065.2829141/1‑150 (MQ=60)
                                                                                                                          GATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGT                             >  SRR19973065.2272329/1‑150 (MQ=60)
                                                                                                                          GATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGT                             >  SRR19973065.2272337/1‑150 (MQ=60)
                                                                                                                           ATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTC                            >  SRR19973065.518287/1‑150 (MQ=60)
                                                                                                                           ATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTC                            <  SRR19973065.2086907/150‑1 (MQ=60)
                                                                                                                            TTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCT                           >  SRR19973065.3235209/1‑150 (MQ=60)
                                                                                                                              TGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGC                         >  SRR19973065.783693/1‑150 (MQ=60)
                                                                                                                               GTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCT                        <  SRR19973065.607600/150‑1 (MQ=60)
                                                                                                                               GTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCT                        <  SRR19973065.3001279/150‑1 (MQ=60)
                                                                                                                               GTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCT                        <  SRR19973065.2384130/150‑1 (MQ=60)
                                                                                                                               GTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCT                        <  SRR19973065.732303/150‑1 (MQ=60)
                                                                                                                               GTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCT                        <  SRR19973065.2651269/150‑1 (MQ=60)
                                                                                                                               GTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCT                        <  SRR19973065.1410277/150‑1 (MQ=60)
                                                                                                                                TTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTG                       <  SRR19973065.971750/150‑1 (MQ=60)
                                                                                                                                    TAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTG                   >  SRR19973065.775404/1‑150 (MQ=60)
                                                                                                                                       ATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCT                <  SRR19973065.590681/150‑1 (MQ=60)
                                                                                                                                       ATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCT                >  SRR19973065.3180232/1‑150 (MQ=60)
                                                                                                                                         CATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAACGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCA              <  SRR19973065.618297/150‑1 (MQ=60)
                                                                                                                                         CATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCA              >  SRR19973065.1417295/1‑150 (MQ=60)
                                                                                                                                         CATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCA              <  SRR19973065.2393310/150‑1 (MQ=60)
                                                                                                                                           TATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATT            <  SRR19973065.1269113/150‑1 (MQ=60)
                                                                                                                                              GGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTAT         >  SRR19973065.2062608/1‑150 (MQ=60)
                                                                                                                                                 TAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTT      <  SRR19973065.1913083/150‑1 (MQ=60)
                                                                                                                                                     TATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTATG  >  SRR19973065.330491/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTTATG  >  CP009273/137766‑138064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑