Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 137,915 G→T 100% intergenic (‑203/‑3) gcd ← / → hpt glucose dehydrogenase/hypoxanthine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273137,9150GT97.4% 731.2 / 7.9 233intergenic (‑203/‑3)gcd/hptglucose dehydrogenase/hypoxanthine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base T (107/120);  total (109/124)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 244 total aligned reads displayed.

AACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTT  >  CP009273/137768‑138061
                                                                                                                                                   |                                                                                                                                                  
aaCATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGatat                                                                                                                                                  >  1:1383753/1‑150 (MQ=255)
aaCATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGatat                                                                                                                                                  <  1:260732/150‑1 (MQ=255)
 aCATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAtata                                                                                                                                                 <  2:5019280/150‑1 (MQ=255)
 aCATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAtata                                                                                                                                                 <  2:4395461/150‑1 (MQ=255)
 aCATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAtata                                                                                                                                                 >  1:2406909/1‑150 (MQ=255)
   aTGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATg                                                                                                                                               >  1:4162717/1‑150 (MQ=255)
   aTGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATg                                                                                                                                               <  2:816399/150‑1 (MQ=255)
   aTGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATg                                                                                                                                               >  2:1920811/1‑150 (MQ=255)
    tgtgtgATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGa                                                                                                                                              <  1:1274191/150‑1 (MQ=255)
       gtgATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAAc                                                                                                                                           <  1:3887173/150‑1 (MQ=255)
          aTCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATa                                                                                                                                        >  2:1820678/1‑150 (MQ=255)
          aTCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATa                                                                                                                                        >  2:3025476/1‑150 (MQ=255)
            cGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACt                                                                                                                                      <  1:2973207/150‑1 (MQ=255)
             gTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTg                                                                                                                                     >  2:2727163/1‑150 (MQ=255)
              tcatcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGt                                                                                                                                    >  2:4112458/1‑150 (MQ=255)
               catcaCAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTa                                                                                                                                   <  2:4721160/150‑1 (MQ=255)
                  cacaATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGaa                                                                                                                                <  1:852741/150‑1 (MQ=255)
                     aaTTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTa                                                                                                                             >  1:4688212/1‑150 (MQ=255)
                      aTTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTaa                                                                                                                            <  2:2373656/150‑1 (MQ=255)
                       ttCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAAt                                                                                                                           <  2:30/150‑1 (MQ=255)
                         cGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGa                                                                                                                         <  1:2494227/150‑1 (MQ=255)
                         cGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGa                                                                                                                         >  1:3352967/1‑150 (MQ=255)
                          gAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGAt                                                                                                                        <  1:1540693/150‑1 (MQ=255)
                            gCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATcc                                                                                                                      <  2:3868692/150‑1 (MQ=255)
                              tttATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATcccc                                                                                                                    <  1:3550654/150‑1 (MQ=255)
                               ttattaACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATcccca                                                                                                                   <  1:378990/150‑2 (MQ=255)
                                tattaACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAGACATACTGTAGAAGTAATGATCCCCGa                                                                                                                  <  1:2506194/150‑1 (MQ=255)
                                 attaACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGaa                                                                                                                 <  2:4618455/150‑1 (MQ=255)
                                  ttaACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAg                                                                                                                >  1:1322908/1‑150 (MQ=255)
                                      cAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGga                                                                                                            >  1:4584140/1‑150 (MQ=255)
                                       aGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGgag                                                                                                           <  2:721713/150‑1 (MQ=255)
                                          ttCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGAtt                                                                                                        <  2:1281017/150‑1 (MQ=255)
                                                gaatgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAgcg                                                                                                  >  1:1899392/1‑150 (MQ=255)
                                                 aatgaatAGTTTTACTGGTATACTGCGTGTCTTGCTCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCACCGAAGCGGAGATTAAAgcgc                                                                                                 >  1:1578390/1‑150 (MQ=255)
                                                  atgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGcgcg                                                                                                <  1:4305584/150‑1 (MQ=255)
                                                  atgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGcgcg                                                                                                >  2:1828265/1‑150 (MQ=255)
                                                   tgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGt                                                                                               >  2:2086073/1‑150 (MQ=255)
                                                   tgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGt                                                                                               >  1:516432/1‑150 (MQ=255)
                                                   tgaatAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGt                                                                                               >  2:4889369/1‑150 (MQ=255)
                                                      atAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATc                                                                                            >  2:1604948/1‑150 (MQ=255)
                                                       tAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCg                                                                                           <  2:2167697/150‑1 (MQ=255)
                                                         gTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGcc                                                                                         <  2:2639495/150‑1 (MQ=255)
                                                           tttACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGa                                                                                       <  1:1367481/150‑1 (MQ=255)
                                                             tACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAAc                                                                                     <  1:4252435/150‑1 (MQ=255)
                                                              aCTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACt                                                                                    <  1:1993110/150‑1 (MQ=255)
                                                              aCTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACt                                                                                    >  2:694184/1‑150 (MQ=255)
                                                                  gTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgt                                                                                >  1:3573018/1‑150 (MQ=255)
                                                                    ataCTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgtcg                                                                              <  2:2472859/150‑1 (MQ=255)
                                                                     taCTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGgtcgt                                                                             <  1:5077540/150‑1 (MQ=255)
                                                                       cTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCa                                                                           <  1:130945/150‑1 (MQ=255)
                                                                        tGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTGAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAg                                                                          >  1:1673298/1‑150 (MQ=255)
                                                                         gCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGa                                                                         <  1:1142981/150‑1 (MQ=255)
                                                                          cGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGAt                                                                        >  2:865344/1‑150 (MQ=255)
                                                                            tgtCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTa                                                                      <  2:3930576/150‑1 (MQ=255)
                                                                             gtCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTAc                                                                     >  1:2630046/1‑150 (MQ=255)
                                                                                    gcTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCgg                                                              >  2:3146488/1‑149 (MQ=255)
                                                                                      tttGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTa                                                            <  2:119027/150‑1 (MQ=255)
                                                                                       ttgttgCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTAc                                                           <  2:587485/150‑1 (MQ=255)
                                                                                        tgttgCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACa                                                          <  2:1120195/150‑1 (MQ=255)
                                                                                         gttgCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACaa                                                         >  1:3255128/1‑150 (MQ=255)
                                                                                          ttgCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACaaa                                                        >  2:3276931/1‑150 (MQ=255)
                                                                                            gCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGa                                                      <  2:3546727/150‑1 (MQ=255)
                                                                                                tGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGc                                                  >  1:580129/1‑150 (MQ=255)
                                                                                                tGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGc                                                  >  1:4890887/1‑150 (MQ=255)
                                                                                                 gCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCg                                                 <  2:2584870/150‑1 (MQ=255)
                                                                                                  ccAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCgg                                                >  1:707624/1‑150 (MQ=255)
                                                                                                    aaaaCCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCa                                              <  2:1669457/150‑1 (MQ=255)
                                                                                                     aaaCCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAg                                             >  2:5064214/1‑150 (MQ=255)
                                                                                                     aaaCCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAg                                             >  2:4763123/1‑150 (MQ=255)
                                                                                                         cTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGat                                         <  2:3648936/150‑1 (MQ=255)
                                                                                                         cTGCCCGTGAGTAGTTATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCTTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGat                                         <  2:5140938/150‑1 (MQ=255)
                                                                                                          tGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGata                                        >  2:1035587/1‑150 (MQ=255)
                                                                                                           gCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGatat                                       >  1:2576632/1‑150 (MQ=255)
                                                                                                           gCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGatat                                       <  2:2050143/150‑1 (MQ=255)
                                                                                                            cccGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATg                                      <  2:1899392/150‑1 (MQ=255)
                                                                                                             ccGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATgg                                     <  1:4862653/150‑1 (MQ=255)
                                                                                                              cGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGt                                    >  2:1386105/1‑150 (MQ=255)
                                                                                                              cGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGt                                    >  1:1529012/1‑150 (MQ=255)
                                                                                                              cGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGt                                    >  2:4557832/1‑150 (MQ=255)
                                                                                                               gTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTg                                   <  1:1697848/150‑1 (MQ=255)
                                                                                                               gTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTg                                   <  1:5032561/150‑1 (MQ=255)
                                                                                                                tGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGc                                  <  1:897896/150‑1 (MQ=255)
                                                                                                                 gCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCt                                 >  1:946156/1‑150 (MQ=255)
                                                                                                                 gCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCt                                 >  1:3986750/1‑150 (MQ=255)
                                                                                                                  cGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtg                                <  2:3055291/150‑1 (MQ=255)
                                                                                                                  cGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtg                                >  2:4671530/1‑150 (MQ=255)
                                                                                                                  cGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtg                                <  1:2814451/150‑1 (MQ=255)
                                                                                                                  cGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtg                                <  1:3632153/150‑1 (MQ=255)
                                                                                                                   gAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCtgg                               <  2:1324925/150‑1 (MQ=255)
                                                                                                                           ttGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctg                       <  2:1694753/150‑1 (MQ=255)
                                                                                                                             gTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctgct                     <  1:4112444/150‑1 (MQ=255)
                                                                                                                             gTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctgct                     >  1:2095758/1‑150 (MQ=255)
                                                                                                                             gTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTctgct                     <  2:504894/150‑1 (MQ=255)
                                                                                                                                     aTCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCt             <  1:1183891/150‑1 (MQ=255)
                                                                                                                                       cATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCa           >  2:1042945/1‑150 (MQ=255)
                                                                                                                                        atatGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATAGGGTGCTGGTGGGTCTGCTGCGTGGCTCAt          >  2:4321178/1‑150 (MQ=255)
                                                                                                                                            ggTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAtttat      >  1:5080398/1‑150 (MQ=255)
                                                                                                                                             gTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAtttatg     <  1:3702834/150‑1 (MQ=255)
                                                                                                                                              ttAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAtttatgt    <  2:3081961/150‑1 (MQ=255)
                                                                                                                                               tAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCAtttatgtt   >  1:2744181/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTT  >  CP009273/137768‑138061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTT  >  CP009273/137768‑138061
                                                                                                                                                   |                                                                                                                                                  
AACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATAT                                                                                                                                                  <  SRR19973063.261510/150‑1 (MQ=60)
AACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATAT                                                                                                                                                  <  SRR19973063.2863400/150‑1 (MQ=60)
AACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATAT                                                                                                                                                  >  SRR19973063.1387934/1‑150 (MQ=60)
 ACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATA                                                                                                                                                 >  SRR19973063.2414610/1‑150 (MQ=60)
 ACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATA                                                                                                                                                 >  SRR19973063.2414691/1‑150 (MQ=60)
  CATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATAT                                                                                                                                                <  SRR19973063.1976621/150‑1 (MQ=60)
   ATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATG                                                                                                                                               >  SRR19973063.4176027/1‑150 (MQ=60)
    TGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGA                                                                                                                                              <  SRR19973063.1277984/150‑1 (MQ=60)
       GTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAAC                                                                                                                                           <  SRR19973063.3899518/150‑1 (MQ=60)
        TGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACA                                                                                                                                          >  SRR19973063.3920582/1‑150 (MQ=60)
        TGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACA                                                                                                                                          >  SRR19973063.1332284/1‑150 (MQ=60)
        TGATCGTCATCACAATTCGAGCTTTATTAACAGATTACGCGAATGAATAGTTTTACTGGTAAACTCCGAGTCTTGCGCTTTGTTGCGGTGACAAAACCTGCCCGTGCTAAGTGATTAGTTTTTAAAACATAGGTTTAGATATATGAAACA                                                                                                                                          >  SRR19973063.5130422/1‑150 (MQ=60)
          ATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATA                                                                                                                                        >  SRR19973063.629731/1‑150 (MQ=60)
           TCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATAC                                                                                                                                       >  SRR19973063.3558091/1‑150 (MQ=60)
            CGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACT                                                                                                                                      <  SRR19973063.2982710/150‑1 (MQ=60)
              TCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGT                                                                                                                                    >  SRR19973063.4878987/1‑150 (MQ=60)
                  CACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAA                                                                                                                                <  SRR19973063.855199/150‑1 (MQ=60)
                   ACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAG                                                                                                                               >  SRR19973063.2588380/1‑150 (MQ=60)
                   ACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAG                                                                                                                               >  SRR19973063.2587771/1‑150 (MQ=60)
                     AATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTA                                                                                                                             >  SRR19973063.4703157/1‑150 (MQ=60)
                      ATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAA                                                                                                                            >  SRR19973063.2154303/1‑150 (MQ=60)
                         CGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGA                                                                                                                         >  SRR19973063.3363642/1‑150 (MQ=60)
                         CGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGA                                                                                                                         <  SRR19973063.2502189/150‑1 (MQ=60)
                         CGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGA                                                                                                                         <  SRR19973063.2502146/150‑1 (MQ=60)
                         CGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGA                                                                                                                         >  SRR19973063.3877957/1‑150 (MQ=60)
                          GAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGAT                                                                                                                        >  SRR19973063.3278656/1‑150 (MQ=60)
                          GAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGAT                                                                                                                        <  SRR19973063.3698886/150‑1 (MQ=60)
                          GAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGAT                                                                                                                        >  SRR19973063.1150548/1‑150 (MQ=60)
                          GAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGAT                                                                                                                        <  SRR19973063.1545414/150‑1 (MQ=60)
                            GCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCC                                                                                                                      >  SRR19973063.1296071/1‑150 (MQ=60)
                              TTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCC                                                                                                                    <  SRR19973063.4393375/150‑1 (MQ=60)
                              TTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCC                                                                                                                    <  SRR19973063.3562110/150‑1 (MQ=60)
                              TTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCC                                                                                                                    <  SRR19973063.3562033/150‑1 (MQ=60)
                               TTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCA                                                                                                                   <  SRR19973063.380071/150‑1 (MQ=60)
                               TTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCA                                                                                                                   <  SRR19973063.380048/150‑1 (MQ=60)
                                TATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAGACATACTGTAGAAGTAATGATCCCCGA                                                                                                                  <  SRR19973063.2514189/150‑1 (MQ=60)
                                TATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGA                                                                                                                  <  SRR19973063.2514164/150‑1 (MQ=60)
                                  TTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAG                                                                                                                >  SRR19973063.1326481/1‑150 (MQ=60)
                                  TTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAG                                                                                                                >  SRR19973063.1326862/1‑150 (MQ=60)
                                  TTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAG                                                                                                                <  SRR19973063.733304/150‑1 (MQ=60)
                                  TTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAG                                                                                                                >  SRR19973063.2536534/1‑150 (MQ=60)
                                      CAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGA                                                                                                            >  SRR19973063.4598815/1‑150 (MQ=60)
                                              GCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAG                                                                                                    <  SRR19973063.2640971/150‑1 (MQ=60)
                                                GAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCG                                                                                                  >  SRR19973063.3753608/1‑150 (MQ=60)
                                                GAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCG                                                                                                  >  SRR19973063.1905333/1‑150 (MQ=60)
                                                 AATGAATAGTTTTACTGGTATACTGCGTGTCTTGCTCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCACCGAAGCGGAGATTAAAGCGC                                                                                                 >  SRR19973063.1583226/1‑150 (MQ=60)
                                                  ATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCG                                                                                                <  SRR19973063.4319262/150‑1 (MQ=60)
                                                   TGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGT                                                                                               >  SRR19973063.517939/1‑150 (MQ=60)
                                                      ATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATC                                                                                            >  SRR19973063.1735043/1‑150 (MQ=60)
                                                           TTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGA                                                                                       <  SRR19973063.2662074/150‑1 (MQ=60)
                                                           TTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGA                                                                                       <  SRR19973063.1371602/150‑1 (MQ=60)
                                                           TTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGA                                                                                       <  SRR19973063.1371899/150‑1 (MQ=60)
                                                             TACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAAC                                                                                     <  SRR19973063.4265978/150‑1 (MQ=60)
                                                             TACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAAC                                                                                     <  SRR19973063.4265966/150‑1 (MQ=60)
                                                              ACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACT                                                                                    <  SRR19973063.1999338/150‑1 (MQ=60)
                                                               CTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTG                                                                                   >  SRR19973063.5063040/1‑150 (MQ=60)
                                                                 GGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCTAACTGGG                                                                                 >  SRR19973063.1449880/1‑150 (MQ=60)
                                                                 GGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGG                                                                                 >  SRR19973063.168466/1‑150 (MQ=60)
                                                                 GGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGG                                                                                 >  SRR19973063.2772286/1‑150 (MQ=60)
                                                                  GTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGT                                                                                >  SRR19973063.3584476/1‑150 (MQ=60)
                                                                     TACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGT                                                                             <  SRR19973063.5093872/150‑1 (MQ=60)
                                                                       CTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCA                                                                           <  SRR19973063.3265677/150‑1 (MQ=60)
                                                                       CTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCA                                                                           <  SRR19973063.131345/150‑1 (MQ=60)
                                                                       CTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCA                                                                           <  SRR19973063.4514087/150‑1 (MQ=60)
                                                                       CTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCA                                                                           <  SRR19973063.131363/150‑1 (MQ=60)
                                                                       CTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCA                                                                           <  SRR19973063.583653/150‑1 (MQ=60)
                                                                        TGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTGAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAG                                                                          >  SRR19973063.1678398/1‑150 (MQ=60)
                                                                         GCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGA                                                                         <  SRR19973063.1146442/150‑1 (MQ=60)
                                                                          CGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGAT                                                                        >  SRR19973063.863975/1‑150 (MQ=60)
                                                                           GTGTCTTGCTCTTTGTTGCTGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATT                                                                       <  SRR19973063.4981430/150‑1 (MQ=60)
                                                                            TGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTA                                                                      <  SRR19973063.4937328/150‑1 (MQ=60)
                                                                            TGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTA                                                                      <  SRR19973063.3628439/150‑1 (MQ=60)
                                                                            TGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTA                                                                      <  SRR19973063.4794945/150‑1 (MQ=60)
                                                                             GTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTAC                                                                     >  SRR19973063.2638433/1‑150 (MQ=60)
                                                                             GTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTAC                                                                     <  SRR19973063.1536178/150‑1 (MQ=60)
                                                                                         GTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAA                                                         >  SRR19973063.3265569/1‑150 (MQ=60)
                                                                                                TGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGC                                                  >  SRR19973063.581837/1‑150 (MQ=60)
                                                                                                TGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGC                                                  >  SRR19973063.4906665/1‑150 (MQ=60)
                                                                                                  CCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGG                                                >  SRR19973063.709703/1‑150 (MQ=60)
                                                                                                  CCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGG                                                <  SRR19973063.1465863/150‑1 (MQ=60)
                                                                                                    AAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTGGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCA                                              >  SRR19973063.299169/1‑150 (MQ=60)
                                                                                                      AACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGC                                            <  SRR19973063.861935/150‑1 (MQ=60)
                                                                                                        CCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGA                                          <  SRR19973063.1569855/150‑1 (MQ=60)
                                                                                                           GCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATAT                                       >  SRR19973063.2584830/1‑150 (MQ=60)
                                                                                                            CCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATG                                      <  SRR19973063.911862/150‑1 (MQ=60)
                                                                                                             CCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGG                                     <  SRR19973063.4878321/150‑1 (MQ=60)
                                                                                                              CGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGT                                    >  SRR19973063.1533700/1‑150 (MQ=60)
                                                                                                               GTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTG                                   <  SRR19973063.1593145/150‑1 (MQ=60)
                                                                                                               GTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTG                                   <  SRR19973063.5048785/150‑1 (MQ=60)
                                                                                                               GTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTG                                   <  SRR19973063.1703023/150‑1 (MQ=60)
                                                                                                               GTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTG                                   >  SRR19973063.2057643/1‑150 (MQ=60)
                                                                                                                TGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGC                                  <  SRR19973063.900516/150‑1 (MQ=60)
                                                                                                                TGCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGC                                  <  SRR19973063.4875970/150‑1 (MQ=60)
                                                                                                                 GCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCT                                 >  SRR19973063.3999530/1‑150 (MQ=60)
                                                                                                                 GCGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCT                                 >  SRR19973063.948987/1‑150 (MQ=60)
                                                                                                                  CGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTG                                <  SRR19973063.2823501/150‑1 (MQ=60)
                                                                                                                  CGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTG                                <  SRR19973063.3643808/150‑1 (MQ=60)
                                                                                                                  CGAAGTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTG                                <  SRR19973063.3643802/150‑1 (MQ=60)
                                                                                                                      GTGATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGG                            <  SRR19973063.1619470/150‑1 (MQ=60)
                                                                                                                         ATTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTC                         <  SRR19973063.972961/150‑1 (MQ=60)
                                                                                                                          TTTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCT                        <  SRR19973063.2091851/150‑1 (MQ=60)
                                                                                                                           TTGTTTTTAAATCATATGGTTAGATATATTAAACATACTGTAGAAGTATTGATCCCGGAAGCGGAGATTAAAGCGCGTATCGCCGTACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCTGCGATATTGTGCTGGTGGGTCTG                       <  SRR19973063.3958034/150‑1 (MQ=60)
                                                                                                                           TTGTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTG                       <  SRR19973063.1370495/150‑1 (MQ=60)
                                                                                                                             GTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCT                     >  SRR19973063.2102306/1‑150 (MQ=60)
                                                                                                                             GTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCT                     <  SRR19973063.4125629/150‑1 (MQ=60)
                                                                                                                             GTTTTTAAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCT                     <  SRR19973063.4125615/150‑1 (MQ=60)
                                                                                                                                   AAATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGG               <  SRR19973063.2629831/150‑1 (MQ=60)
                                                                                                                                   AAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGG               >  SRR19973063.427727/1‑150 (MQ=60)
                                                                                                                                    AATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGC              >  SRR19973063.180287/1‑150 (MQ=60)
                                                                                                                                    AATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGC              <  SRR19973063.4635566/150‑1 (MQ=60)
                                                                                                                                     ATCATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCT             <  SRR19973063.1187458/150‑1 (MQ=60)
                                                                                                                                       CATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCA           >  SRR19973063.40480/1‑150 (MQ=60)
                                                                                                                                       CATATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCA           <  SRR19973063.549276/150‑1 (MQ=60)
                                                                                                                                          ATGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTT        >  SRR19973063.5100110/1‑150 (MQ=60)
                                                                                                                                           TGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTA       <  SRR19973063.1265787/150‑1 (MQ=60)
                                                                                                                                           TGGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTA       >  SRR19973063.2849994/1‑150 (MQ=60)
                                                                                                                                            GGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTAT      >  SRR19973063.5096738/1‑150 (MQ=60)
                                                                                                                                            GGTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTAT      >  SRR19973063.5096665/1‑150 (MQ=60)
                                                                                                                                             GTTAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATG     <  SRR19973063.3714681/150‑1 (MQ=60)
                                                                                                                                               TAGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTT   >  SRR19973063.2753037/1‑150 (MQ=60)
                                                                                                                                                AGATATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTT  >  SRR19973063.4225524/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                  
AACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTTGCGGTGCCAAAACCTGCCCGTGCGAAGTGATTTGTTTTTAAATCATATGGTTAGAGATATGAAACATACTGTAGAAGTAATGATCCCCGAAGCGGAGATTAAAGCGCGTATCGCCGAACTGGGTCGTCAGATTACTGAGCGTTACAAAGACAGCGGCAGCGATATGGTGCTGGTGGGTCTGCTGCGTGGCTCATTTATGTTT  >  CP009273/137768‑138061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑