Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 747,973 C→A 100% P93P (CCG→CCT)  ybgD ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273747,9730CA95.7% 630.3 / 17.5 209P93P (CCG→CCT) ybgDputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (3/6);  new base A (80/120);  total (83/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 210 total aligned reads displayed.

CTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGA  >  CP009273/747828‑748120
                                                                                                                                                 |                                                                                                                                                   
cTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAgg                                                                                                                                                   <  2:1173137/148‑1 (MQ=255)
   aaCCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAt                                                                                                                                                >  2:2609350/1‑148 (MQ=255)
    aCCTAATACTATGTTACCTTCATTTTTGTACATCAGTCGTACACCGCCCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAAAAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCAAACTTTGGATACAGGCAtt                                                                                                                                               <  1:2442154/148‑1 (MQ=255)
     ccTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTc                                                                                                                                              <  1:800353/148‑1 (MQ=255)
     ccTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTc                                                                                                                                              <  1:2981819/148‑1 (MQ=255)
        aaTACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGc                                                                                                                                           >  1:1211930/1‑148 (MQ=255)
        aaTACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGc                                                                                                                                           <  2:1637483/148‑1 (MQ=255)
           aCGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTAc                                                                                                                                        <  1:2431489/148‑1 (MQ=255)
           aCGATGTTACCGACATTTTTGACAATCAGTCGTACAACGACCCCAGTTCCTTCGCCTGCACTGGTGTTGATCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTAACTACAACTTTGGATACAGGCAATCAGCTAc                                                                                                                                        >  2:1923431/1‑148 (MQ=255)
              aTGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGt                                                                                                                                     <  1:3449994/148‑1 (MQ=255)
                 ttACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGt                                                                                                                                  <  2:3546280/148‑1 (MQ=255)
                 ttACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGt                                                                                                                                  <  2:3546341/148‑1 (MQ=255)
                       tCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAg                                                                                                                            <  2:2808175/148‑1 (MQ=255)
                       tCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAg                                                                                                                            >  2:1851481/1‑148 (MQ=255)
                        cATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgc                                                                                                                           <  1:1522463/148‑1 (MQ=255)
                            tttGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcaggc                                                                                                                       >  2:2461527/1‑148 (MQ=255)
                                tCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGAt                                                                                                                   >  1:150450/1‑148 (MQ=255)
                                  cATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATca                                                                                                                 >  2:3408579/1‑148 (MQ=255)
                                    tCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATcaca                                                                                                               >  2:1055163/1‑148 (MQ=255)
                                    tCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATcaca                                                                                                               >  2:2795597/1‑148 (MQ=255)
                                     cAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAg                                                                                                              <  2:441216/148‑1 (MQ=255)
                                           tACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAGTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATc                                                                                                        <  1:1382644/148‑1 (MQ=255)
                                            acacCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCa                                                                                                       >  1:1072502/1‑148 (MQ=255)
                                              acCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACC‑TTGTCAGAAGCAGGCAGATCACAGTTGATCAAGc                                                                                                    <  2:842665/148‑1 (MQ=255)
                                                cGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCg                                                                                                   >  1:456903/1‑148 (MQ=255)
                                                 gACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGa                                                                                                  <  1:369567/148‑1 (MQ=255)
                                                  aCCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGaa                                                                                                 <  1:3523611/148‑1 (MQ=255)
                                                     ccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGt                                                                                              <  2:2321426/148‑1 (MQ=255)
                                                      cAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTc                                                                                             >  1:1906048/1‑148 (MQ=255)
                                                       aGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCg                                                                                            >  1:1819967/1‑148 (MQ=255)
                                                       aGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCg                                                                                            <  1:433429/148‑1 (MQ=255)
                                                           gCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAATCGAAAGTCGACgg                                                                                        >  1:1093494/1‑148 (MQ=255)
                                                              tCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCa                                                                                     <  2:2335000/148‑1 (MQ=255)
                                                              tCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCa                                                                                     <  2:2056590/148‑1 (MQ=255)
                                                              tCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCa                                                                                     <  2:648995/148‑1 (MQ=255)
                                                                 ccTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACtt                                                                                  <  1:1620148/148‑1 (MQ=255)
                                                                  cTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACttt                                                                                 <  2:3056403/148‑1 (MQ=255)
                                                                  cTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACttt                                                                                 <  2:3094832/148‑1 (MQ=255)
                                                                     cACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAtt                                                                              >  2:2573704/1‑148 (MQ=255)
                                                                      aCTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTg                                                                             >  1:701577/1‑148 (MQ=255)
                                                                       cTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGc                                                                            <  2:1722394/148‑1 (MQ=255)
                                                                        tGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCt                                                                           <  1:20229/148‑1 (MQ=255)
                                                                           tgtTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTatg                                                                        <  2:1569220/148‑1 (MQ=255)
                                                                            gtTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTatga                                                                       <  1:264297/148‑1 (MQ=255)
                                                                             ttGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTatgat                                                                      >  2:1359957/1‑148 (MQ=255)
                                                                                 tCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTc                                                                  <  1:1893272/148‑1 (MQ=255)
                                                                                  caacaaAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTCGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTcc                                                                 <  2:1118905/148‑1 (MQ=255)
                                                                                     caaAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCgg                                                              >  2:3411571/1‑148 (MQ=255)
                                                                                       aaGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGtt                                                            <  1:887052/148‑1 (MQ=255)
                                                                                        aGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGttt                                                           <  2:335607/148‑1 (MQ=255)
                                                                                        aGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGttt                                                           >  2:1528029/1‑148 (MQ=255)
                                                                                               gCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATggg                                                    <  1:2249239/148‑1 (MQ=255)
                                                                                                cACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGt                                                   <  1:3466214/148‑1 (MQ=255)
                                                                                                  ccAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTg                                                 <  1:2287805/148‑1 (MQ=255)
                                                                                                   cAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGt                                                <  2:150450/148‑1 (MQ=255)
                                                                                                   cAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGt                                                >  2:704440/1‑148 (MQ=255)
                                                                                                       tGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTg                                            >  2:159760/1‑148 (MQ=255)
                                                                                                       tGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTGGTCGTg                                            >  2:1250823/1‑148 (MQ=255)
                                                                                                         tCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGAc                                          <  1:1284863/148‑1 (MQ=255)
                                                                                                         tCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGAc                                          >  1:1478652/1‑148 (MQ=255)
                                                                                                          cTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACt                                         <  2:701577/148‑1 (MQ=255)
                                                                                                              gCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTc                                     <  1:736687/148‑1 (MQ=255)
                                                                                                              gCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTc                                     <  1:1337072/148‑1 (MQ=255)
                                                                                                                cGTGCTATCGAAGGTTACGCCAACTTTGGAGACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCa                                   <  2:1824745/148‑1 (MQ=255)
                                                                                                                  tGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAg                                 <  2:2317971/148‑1 (MQ=255)
                                                                                                                   gCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGa                                <  1:3332514/148‑1 (MQ=255)
                                                                                                                   gCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGa                                <  1:2525510/148‑1 (MQ=255)
                                                                                                                    cTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGAt                               >  1:917539/1‑148 (MQ=255)
                                                                                                                     tATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATc                              <  1:3508468/148‑1 (MQ=255)
                                                                                                                      aTCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCt                             >  1:273967/1‑148 (MQ=255)
                                                                                                                      aTCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCt                             <  2:3025503/148‑1 (MQ=255)
                                                                                                                      aTCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCt                             <  2:1046068/148‑1 (MQ=255)
                                                                                                                      aTCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCt                             <  1:2461527/148‑1 (MQ=255)
                                                                                                                       tCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTa                            <  2:544074/148‑1 (MQ=255)
                                                                                                                       tCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTa                            <  1:3298356/148‑1 (MQ=255)
                                                                                                                       tCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTa                            >  1:1718877/1‑148 (MQ=255)
                                                                                                                        cGAAGGTTACGCCAACTTTCGATACAGGCATTCCGAAACCGTTGACAGAAGCACGCACATCACAGATGATCAAGCCAATGTCTACGGACACTTTATTCAAATGATGCTCACGGTTTATATGGTTTGTAGTTACTAGACCAAGATCAAt                           >  2:422805/1‑148 (MQ=255)
                                                                                                                         gAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATg                          <  1:2687431/148‑1 (MQ=255)
                                                                                                                         gAAGGTTACGCCAACTGTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATg                          <  2:3329635/148‑1 (MQ=255)
                                                                                                                          aaGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGt                         >  1:2199706/1‑148 (MQ=255)
                                                                                                                            ggTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGttt                       >  2:2256235/1‑148 (MQ=255)
                                                                                                                            ggTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGttt                       <  1:59547/148‑1 (MQ=255)
                                                                                                                            ggTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGttt                       <  2:139947/148‑1 (MQ=255)
                                                                                                                               tACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTa                    <  2:2460614/148‑1 (MQ=255)
                                                                                                                               tACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTa                    <  2:3155668/148‑1 (MQ=255)
                                                                                                                               tACGCCAACTTTGGATACAGGCAGTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTa                    <  2:3155589/148‑1 (MQ=255)
                                                                                                                                 cGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATc                  >  2:3169450/1‑148 (MQ=255)
                                                                                                                                   ccAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCaa                <  2:2545353/148‑1 (MQ=255)
                                                                                                                                    cAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATAGCTATGATGCTCCCGCTTTAGATGGGTTGTCGTGACTTGTCCAACATCTATGTTTTTATCAat               >  2:3211846/1‑148 (MQ=255)
                                                                                                                                     aaCTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAata              >  2:1528835/1‑148 (MQ=255)
                                                                                                                                      aCTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAatat             >  2:3283941/1‑148 (MQ=255)
                                                                                                                                      aCTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAatat             <  2:2684772/148‑1 (MQ=255)
                                                                                                                                      aCTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAatat             >  2:1762424/1‑148 (MQ=255)
                                                                                                                                       cTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtc            <  1:2036740/148‑1 (MQ=255)
                                                                                                                                       cTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtc            >  1:2478436/1‑148 (MQ=255)
                                                                                                                                       cTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtc            >  2:166630/1‑148 (MQ=255)
                                                                                                                                        tttGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtct           >  1:1018374/1‑148 (MQ=255)
                                                                                                                                           ggATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtcttct        <  2:2287910/148‑1 (MQ=255)
                                                                                                                                              tACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGa     >  1:2691381/1‑148 (MQ=255)
                                                                                                                                                 cGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGa  >  2:933001/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGA  >  CP009273/747828‑748120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAAT  >  CP009273/747825‑748122
                                                                                                                                                    |                                                                                                                                                     
CGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAG                                                                                                                                                      <  SRR19973067.48137/150‑1 (MQ=60)
CGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAG                                                                                                                                                      <  SRR19973067.48099/150‑1 (MQ=60)
 GGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGG                                                                                                                                                     <  SRR19973067.2429305/150‑1 (MQ=60)
   CTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCA                                                                                                                                                   <  SRR19973067.2844300/150‑1 (MQ=60)
   CTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCA                                                                                                                                                   <  SRR19973067.1490824/150‑1 (MQ=60)
    TGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAT                                                                                                                                                  <  SRR19973067.2580352/150‑1 (MQ=60)
      AACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTC                                                                                                                                                <  SRR19973067.1029037/150‑1 (MQ=60)
       ACCTAATACTATGTTACCTTCATTTTTGTACATCAGTCGTACACCGCCCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAAAAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCAAACTTTGGATACAGGCATTCC                                                                                                                                               <  SRR19973067.2458191/150‑1 (MQ=60)
       ACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCC                                                                                                                                               <  SRR19973067.662212/150‑1 (MQ=60)
        CCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCG                                                                                                                                              <  SRR19973067.805863/150‑1 (MQ=60)
        CCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCG                                                                                                                                              <  SRR19973067.3000653/150‑1 (MQ=60)
        CCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTAAGCCAACTTTGGATACAGGCATTCCG                                                                                                                                              >  SRR19973067.951472/1‑150 (MQ=60)
         CTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGC                                                                                                                                             >  SRR19973067.1219933/1‑150 (MQ=60)
         CTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGC                                                                                                                                             >  SRR19973067.3075867/1‑150 (MQ=60)
              ACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCG                                                                                                                                        <  SRR19973067.2448903/150‑1 (MQ=60)
              ACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCG                                                                                                                                        <  SRR19973067.2447450/150‑1 (MQ=60)
              ACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCG                                                                                                                                        <  SRR19973067.2447432/150‑1 (MQ=60)
                 ATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTG                                                                                                                                     <  SRR19973067.3471938/150‑1 (MQ=60)
                    TTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCA                                                                                                                                  <  SRR19973067.1907318/150‑1 (MQ=60)
                           CATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAG                                                                                                                           <  SRR19973067.1532675/150‑1 (MQ=60)
                                 TGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGAT                                                                                                                     >  SRR19973067.151739/1‑150 (MQ=60)
                                  GTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATC                                                                                                                    >  SRR19973067.1708745/1‑150 (MQ=60)
                                    CCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCAC                                                                                                                  <  SRR19973067.1161921/150‑1 (MQ=60)
                                            CGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATC                                                                                                          <  SRR19973067.2713897/150‑1 (MQ=60)
                                             GTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCA                                                                                                         >  SRR19973067.1079857/1‑150 (MQ=60)
                                              TACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAGTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAA                                                                                                        <  SRR19973067.1392057/150‑1 (MQ=60)
                                                 ACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCG                                                                                                     >  SRR19973067.460066/1‑150 (MQ=60)
                                                 ACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCG                                                                                                     <  SRR19973067.2102663/150‑1 (MQ=60)
                                                    GACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAAT                                                                                                  <  SRR19973067.372106/150‑1 (MQ=60)
                                                    GACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAAT                                                                                                  <  SRR19973067.372141/150‑1 (MQ=60)
                                                     ACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATG                                                                                                 <  SRR19973067.3545817/150‑1 (MQ=60)
                                                     ACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATG                                                                                                 <  SRR19973067.3545897/150‑1 (MQ=60)
                                                     ACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATG                                                                                                 <  SRR19973067.22578/150‑1 (MQ=60)
                                                       CCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTC                                                                                               >  SRR19973067.190521/1‑150 (MQ=60)
                                                       CCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTC                                                                                               >  SRR19973067.1919042/1‑150 (MQ=60)
                                                       CCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTC                                                                                               >  SRR19973067.1919108/1‑150 (MQ=60)
                                                        CCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCG                                                                                              >  SRR19973067.1832303/1‑150 (MQ=60)
                                                        CCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCG                                                                                              <  SRR19973067.783376/150‑1 (MQ=60)
                                                        CCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCG                                                                                              >  SRR19973067.1830765/1‑150 (MQ=60)
                                                          AGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGAC                                                                                            <  SRR19973067.436443/150‑1 (MQ=60)
                                                            TTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAATCGAAAGTCGACGG                                                                                          >  SRR19973067.1100956/1‑150 (MQ=60)
                                                                    CCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTA                                                                                  <  SRR19973067.1631268/150‑1 (MQ=60)
                                                                      TGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATT                                                                                >  SRR19973067.1528204/1‑150 (MQ=60)
                                                                       GCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTG                                                                               >  SRR19973067.706430/1‑150 (MQ=60)
                                                                       GCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTG                                                                               >  SRR19973067.706488/1‑150 (MQ=60)
                                                                       GCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTG                                                                               >  SRR19973067.2505501/1‑150 (MQ=60)
                                                                           TGGTGTTGCTCATCTAAGGCGTAGCATCAGTTTTCTTAGCGGTGCTATCTAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCATAAGCAGGCAGATCAGAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTAT                                                                           <  SRR19973067.20573/150‑1 (MQ=60)
                                                                           TGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTAT                                                                           <  SRR19973067.20532/150‑1 (MQ=60)
                                                                           TGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTAT                                                                           >  SRR19973067.845800/1‑150 (MQ=60)
                                                                           TGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTAT                                                                           <  SRR19973067.1241761/150‑1 (MQ=60)
                                                                               GTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATG                                                                       <  SRR19973067.267182/150‑1 (MQ=60)
                                                                               GTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATG                                                                       <  SRR19973067.266090/150‑1 (MQ=60)
                                                                                    TCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCC                                                                  <  SRR19973067.1906193/150‑1 (MQ=60)
                                                                                     CAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCG                                                                 <  SRR19973067.31151/150‑1 (MQ=60)
                                                                                         AAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTT                                                             <  SRR19973067.67035/150‑1 (MQ=60)
                                                                                          AAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTA                                                            <  SRR19973067.892938/150‑1 (MQ=60)
                                                                                                 AGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGT                                                     >  SRR19973067.923394/1‑150 (MQ=60)
                                                                                                  GCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTT                                                    <  SRR19973067.461337/150‑1 (MQ=60)
                                                                                                  GCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTT                                                    <  SRR19973067.2264180/150‑1 (MQ=60)
                                                                                                   CACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTG                                                   <  SRR19973067.3488246/150‑1 (MQ=60)
                                                                                                   CACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTG                                                   <  SRR19973067.3488162/150‑1 (MQ=60)
                                                                                                    ACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGT                                                  >  SRR19973067.2375996/1‑150 (MQ=60)
                                                                                                    ACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGT                                                  <  SRR19973067.1606569/150‑1 (MQ=60)
                                                                                                     CCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTC                                                 >  SRR19973067.3521278/1‑150 (MQ=60)
                                                                                                     CCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTC                                                 <  SRR19973067.2302929/150‑1 (MQ=60)
                                                                                                         TTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGA                                             >  SRR19973067.2481812/1‑150 (MQ=60)
                                                                                                          TGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGAC                                            >  SRR19973067.1488475/1‑150 (MQ=60)
                                                                                                          TGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGAC                                            >  SRR19973067.1488022/1‑150 (MQ=60)
                                                                                                            TCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTT                                          <  SRR19973067.1293619/150‑1 (MQ=60)
                                                                                                             CTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTG                                         <  SRR19973067.108916/150‑1 (MQ=60)
                                                                                                                AGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCC                                      <  SRR19973067.2289921/150‑1 (MQ=60)
                                                                                                                 GCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCA                                     <  SRR19973067.1346141/150‑1 (MQ=60)
                                                                                                                 GCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCA                                     >  SRR19973067.1590784/1‑150 (MQ=60)
                                                                                                                 GCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCA                                     <  SRR19973067.741801/150‑1 (MQ=60)
                                                                                                                 GCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCA                                     <  SRR19973067.1346219/150‑1 (MQ=60)
                                                                                                                  CCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAA                                    <  SRR19973067.1379496/150‑1 (MQ=60)
                                                                                                                     TGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGAT                                 >  SRR19973067.923669/1‑150 (MQ=60)
                                                                                                                      GCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATC                                <  SRR19973067.2542063/150‑1 (MQ=60)
                                                                                                                      GCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATC                                <  SRR19973067.3353601/150‑1 (MQ=60)
                                                                                                                       CTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCT                               >  SRR19973067.275787/1‑150 (MQ=60)
                                                                                                                        TATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTA                              <  SRR19973067.3530688/150‑1 (MQ=60)
                                                                                                                        TATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTA                              >  SRR19973067.1730654/1‑150 (MQ=60)
                                                                                                                         ATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTAT                             <  SRR19973067.2477710/150‑1 (MQ=60)
                                                                                                                         ATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTAT                             <  SRR19973067.2477589/150‑1 (MQ=60)
                                                                                                                          TCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATG                            <  SRR19973067.3319126/150‑1 (MQ=60)
                                                                                                                           CGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGT                           >  SRR19973067.2214383/1‑150 (MQ=60)
                                                                                                                            GAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTT                          <  SRR19973067.2704677/150‑1 (MQ=60)
                                                                                                                            GAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTT                          <  SRR19973067.2704825/150‑1 (MQ=60)
                                                                                                                               GGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTT                       <  SRR19973067.414235/150‑1 (MQ=60)
                                                                                                                               GGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTT                       <  SRR19973067.60278/150‑1 (MQ=60)
                                                                                                                                      CCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATA                <  SRR19973067.456631/150‑1 (MQ=60)
                                                                                                                                       CAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAT               <  SRR19973067.3389573/150‑1 (MQ=60)
                                                                                                                                        AACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATC              >  SRR19973067.2494732/1‑150 (MQ=60)
                                                                                                                                         ACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCT             >  SRR19973067.1025366/1‑150 (MQ=60)
                                                                                                                                          CTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTT            <  SRR19973067.2050373/150‑1 (MQ=60)
                                                                                                                                               GATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGA       >  SRR19973067.2708675/1‑150 (MQ=60)
                                                                                                                                                ATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGAT      <  SRR19973067.3350142/150‑1 (MQ=60)
                                                                                                                                                   CAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAA   <  SRR19973067.3430276/150‑1 (MQ=60)
                                                                                                                                                    AGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAAT  >  SRR19973067.1225343/1‑150 (MQ=60)
                                                                                                                                                    AGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAAT  >  SRR19973067.2151636/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAAT  >  CP009273/747825‑748122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑