Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 747,973 C→A P93P (CCG→CCT)  ybgD ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273747,9730CA100.0% 726.4 / NA 217P93P (CCG→CCT) ybgDputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (107/110);  total (107/110)

Only 100 of 217 total aligned reads displayed.

TGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATG  >  CP009273/747829‑748119
                                                                                                                                                |                                                                                                                                                  
tGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAt                                                                                                                                               <  2:812564/150‑1 (MQ=255)
 gAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAtt                                                                                                                                              <  2:360108/150‑1 (MQ=255)
 gAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAtt                                                                                                                                              <  2:485809/150‑1 (MQ=255)
      tAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCt                                                                                                                                         <  2:2430003/150‑1 (MQ=255)
       aaTACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTa                                                                                                                                        >  2:452615/1‑150 (MQ=255)
         tACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTAcc                                                                                                                                      >  1:3441183/1‑150 (MQ=255)
           cGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGt                                                                                                                                    >  2:2762789/1‑150 (MQ=255)
              tGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGt                                                                                                                                 <  1:2564012/150‑1 (MQ=255)
              tGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGt                                                                                                                                 <  1:656887/150‑1 (MQ=255)
               gTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTc                                                                                                                                >  2:2508909/1‑150 (MQ=255)
                 tACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAg                                                                                                                              >  2:2941348/1‑150 (MQ=255)
                  aCCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGa                                                                                                                             >  1:1958094/1‑150 (MQ=255)
                    cGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAg                                                                                                                           <  2:3634471/150‑1 (MQ=255)
                    cGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAg                                                                                                                           >  2:3294623/1‑150 (MQ=255)
                    cGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAg                                                                                                                           <  1:401449/150‑1 (MQ=255)
                    cGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAg                                                                                                                           >  2:3473640/1‑150 (MQ=255)
                     gTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgc                                                                                                                          >  2:3237922/1‑150 (MQ=255)
                       cATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcag                                                                                                                        <  2:303887/150‑1 (MQ=255)
                        aTTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcagg                                                                                                                       <  2:2004533/150‑1 (MQ=255)
                         tttttGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcaggc                                                                                                                      <  1:2318706/150‑1 (MQ=255)
                             tGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGAt                                                                                                                  >  1:2022494/1‑150 (MQ=255)
                              gTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATc                                                                                                                 <  2:845868/150‑1 (MQ=255)
                               tCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATca                                                                                                                >  1:617400/1‑150 (MQ=255)
                               tCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATca                                                                                                                >  1:3258997/1‑150 (MQ=255)
                                ccATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATcac                                                                                                               >  1:1672497/1‑150 (MQ=255)
                                 cATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATcaca                                                                                                              >  1:817897/1‑150 (MQ=255)
                                  aTCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAg                                                                                                             >  2:530796/1‑150 (MQ=255)
                                    cAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGtt                                                                                                           <  2:1912630/150‑1 (MQ=255)
                                     aGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTg                                                                                                          >  1:1429828/1‑150 (MQ=255)
                                      gTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGa                                                                                                         <  1:1392813/150‑1 (MQ=255)
                                      gTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGa                                                                                                         <  1:2762789/150‑1 (MQ=255)
                                      gTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGa                                                                                                         <  2:186130/150‑1 (MQ=255)
                                        cGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATc                                                                                                       <  1:1723658/150‑1 (MQ=255)
                                        cGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATc                                                                                                       >  1:3053588/1‑150 (MQ=255)
                                         gTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCa                                                                                                      <  2:1309371/150‑1 (MQ=255)
                                         gTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCa                                                                                                      <  1:3637703/150‑1 (MQ=255)
                                           acacCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAg                                                                                                    >  2:2390765/1‑150 (MQ=255)
                                            cacCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGc                                                                                                   <  1:1167801/150‑1 (MQ=255)
                                             acCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCg                                                                                                  <  2:1648764/150‑1 (MQ=255)
                                             acCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCg                                                                                                  >  1:2076554/1‑150 (MQ=255)
                                              ccGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGa                                                                                                 >  2:3261107/1‑150 (MQ=255)
                                                gACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAAt                                                                                               <  2:1811251/150‑1 (MQ=255)
                                                gACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAAt                                                                                               >  2:3700671/1‑150 (MQ=255)
                                                gACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAAt                                                                                               <  2:2888189/150‑1 (MQ=255)
                                                 aCCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATg                                                                                              <  1:3318427/150‑1 (MQ=255)
                                                  ccccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGt                                                                                             >  1:2237004/1‑150 (MQ=255)
                                                   cccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGACTGTc                                                                                            >  2:1020327/1‑150 (MQ=255)
                                                    ccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCg                                                                                           <  1:897071/150‑1 (MQ=255)
                                                    ccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCg                                                                                           >  2:2630580/1‑150 (MQ=255)
                                                    ccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCg                                                                                           >  1:2026346/1‑150 (MQ=255)
                                                    ccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCg                                                                                           <  1:741813/150‑1 (MQ=255)
                                                    ccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCg                                                                                           <  1:2647362/150‑1 (MQ=255)
                                                        ttGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACgg                                                                                       <  2:467361/150‑1 (MQ=255)
                                                          gCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGcc                                                                                     <  2:2070928/150‑1 (MQ=255)
                                                          gCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGcc                                                                                     <  2:2282867/150‑1 (MQ=255)
                                                          gCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGcc                                                                                     >  1:2275479/1‑150 (MQ=255)
                                                          gCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGcc                                                                                     <  2:2283421/150‑1 (MQ=255)
                                                             tCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACt                                                                                  >  1:2752440/1‑150 (MQ=255)
                                                               gCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACttt                                                                                <  2:3153640/150‑1 (MQ=255)
                                                                ccTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTa                                                                               >  1:2152392/1‑150 (MQ=255)
                                                                ccTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTa                                                                               >  1:3533683/1‑150 (MQ=255)
                                                                 cTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAt                                                                              >  1:2991958/1‑150 (MQ=255)
                                                                 cTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAt                                                                              >  2:771181/1‑150 (MQ=255)
                                                                  tGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCTGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAtt                                                                             <  2:3498132/150‑1 (MQ=255)
                                                                      cTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTa                                                                         >  1:2601414/1‑150 (MQ=255)
                                                                      cTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTa                                                                         >  1:2464004/1‑150 (MQ=255)
                                                                      cTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTa                                                                         <  2:1203334/150‑1 (MQ=255)
                                                                        ggTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTatg                                                                       >  1:2399540/1‑150 (MQ=255)
                                                                            ttGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGc                                                                   >  1:3477973/1‑150 (MQ=255)
                                                                              gCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTc                                                                 >  1:3185670/1‑150 (MQ=255)
                                                                                    ttaaGTCGGAGCTCTAGTTGTCTTAGGCGTGCTATTGTAGGTTTCGCCATCTTTGGTTACAGGCATTCCGCTACCGTTGTCAGATGCAGGCAGATGACAGTTGATCTAGCGTTTGTCGAGGGCCACTTTATTGCTATGATGCTCCCGGtt                                                           <  1:1224536/148‑1 (MQ=255)
                                                                                     aaaGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGttt                                                          <  1:1311366/150‑1 (MQ=255)
                                                                                       aGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTtat                                                        <  1:209781/150‑1 (MQ=255)
                                                                                            tAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATggg                                                   >  2:657217/1‑150 (MQ=255)
                                                                                              gCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGtt                                                 >  2:950566/1‑150 (MQ=255)
                                                                                               cACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTGTATTGCTATGATGCTCCCGGTGTATATGGGTTg                                                >  2:3757604/1‑150 (MQ=255)
                                                                                                aCCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGt                                               >  2:1542636/1‑150 (MQ=255)
                                                                                                   aGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGt                                            >  1:1921295/1‑150 (MQ=255)
                                                                                                       gTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACt                                        <  1:2937445/150‑1 (MQ=255)
                                                                                                          ttAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGt                                     <  1:1865663/150‑1 (MQ=255)
                                                                                                            aGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTcc                                   <  2:1577961/150‑1 (MQ=255)
                                                                                                                 tGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGAt                              <  1:3700671/150‑1 (MQ=255)
                                                                                                                   cTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCt                            <  1:2391853/150‑1 (MQ=255)
                                                                                                                   cTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCt                            <  1:866429/150‑1 (MQ=255)
                                                                                                                     aTCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTAt                          <  1:542333/150‑1 (MQ=255)
                                                                                                                      tCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATg                         >  2:133922/1‑150 (MQ=255)
                                                                                                                       cGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGt                        >  2:3684952/1‑150 (MQ=255)
                                                                                                                        gAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGtt                       <  1:1369819/150‑1 (MQ=255)
                                                                                                                         aaGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGttt                      >  2:778248/1‑150 (MQ=255)
                                                                                                                          aGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGtttt                     <  1:530796/150‑1 (MQ=255)
                                                                                                                            gTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTa                   <  2:1299269/150‑1 (MQ=255)
                                                                                                                                 gCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAat              >  2:1370844/1‑150 (MQ=255)
                                                                                                                                 gCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAat              >  2:254536/1‑150 (MQ=255)
                                                                                                                                     aCTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtct          <  1:287898/150‑1 (MQ=255)
                                                                                                                                      cTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtctt         <  2:2026346/150‑1 (MQ=255)
                                                                                                                                      cTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtctt         <  1:1805022/150‑1 (MQ=255)
                                                                                                                                       tttGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtcttc        <  1:2631593/150‑1 (MQ=255)
                                                                                                                                        ttGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtcttct       <  1:585206/150‑1 (MQ=255)
                                                                                                                                        ttGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtcttct       >  1:1505942/1‑150 (MQ=255)
                                                                                                                                         tGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTg      >  2:1617290/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATG  >  CP009273/747829‑748119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAAT  >  CP009273/747829‑748122
                                                                                                                                                |                                                                                                                                                     
TGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAAGCAT                                                                                                                                                  <  SRR19973061.726513/150‑1 (MQ=60)
 GAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATT                                                                                                                                                 <  SRR19973061.271003/150‑1 (MQ=60)
  AACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTC                                                                                                                                                <  SRR19973061.2225406/150‑1 (MQ=60)
  AACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTC                                                                                                                                                <  SRR19973061.3083265/150‑1 (MQ=60)
     CTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGC                                                                                                                                             <  SRR19973061.2421980/150‑1 (MQ=60)
       AATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTA                                                                                                                                           >  SRR19973061.1571511/1‑150 (MQ=60)
         TACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACC                                                                                                                                         >  SRR19973061.3450013/1‑150 (MQ=60)
            GATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTT                                                                                                                                      <  SRR19973061.3742403/150‑1 (MQ=60)
            GATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTT                                                                                                                                      <  SRR19973061.2830742/150‑1 (MQ=60)
             ATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTG                                                                                                                                     <  SRR19973061.667387/150‑1 (MQ=60)
              TGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGT                                                                                                                                    <  SRR19973061.2570558/150‑1 (MQ=60)
              TGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGT                                                                                                                                    <  SRR19973061.658575/150‑1 (MQ=60)
                 TACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAG                                                                                                                                 <  SRR19973061.1767385/150‑1 (MQ=60)
                  ACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGA                                                                                                                                >  SRR19973061.1962989/1‑150 (MQ=60)
                  ACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGA                                                                                                                                >  SRR19973061.337039/1‑150 (MQ=60)
                    CGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAG                                                                                                                              >  SRR19973061.2121953/1‑150 (MQ=60)
                    CGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAG                                                                                                                              <  SRR19973061.2201584/150‑1 (MQ=60)
                    CGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAG                                                                                                                              <  SRR19973061.402542/150‑1 (MQ=60)
                    CGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAG                                                                                                                              <  SRR19973061.570426/150‑1 (MQ=60)
                        ATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGG                                                                                                                          >  SRR19973061.3488437/1‑150 (MQ=60)
                         TTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGC                                                                                                                         <  SRR19973061.2324592/150‑1 (MQ=60)
                          TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCA                                                                                                                        <  SRR19973061.2335829/150‑1 (MQ=60)
                             TGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGAT                                                                                                                     >  SRR19973061.2027572/1‑150 (MQ=60)
                             TGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGAT                                                                                                                     <  SRR19973061.2097264/150‑1 (MQ=60)
                               TCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCA                                                                                                                   >  SRR19973061.618982/1‑150 (MQ=60)
                               TCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCA                                                                                                                   >  SRR19973061.618999/1‑150 (MQ=60)
                               TCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCA                                                                                                                   >  SRR19973061.3267391/1‑150 (MQ=60)
                                CCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCAC                                                                                                                  >  SRR19973061.1676697/1‑150 (MQ=60)
                                 CATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACA                                                                                                                 >  SRR19973061.1910223/1‑150 (MQ=60)
                                 CATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACA                                                                                                                 >  SRR19973061.820035/1‑150 (MQ=60)
                                 CATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACA                                                                                                                 >  SRR19973061.820095/1‑150 (MQ=60)
                                   TCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGT                                                                                                               <  SRR19973061.984761/150‑1 (MQ=60)
                                     AGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTG                                                                                                             >  SRR19973061.1433426/1‑150 (MQ=60)
                                      GTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGA                                                                                                            <  SRR19973061.2769904/150‑1 (MQ=60)
                                      GTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGA                                                                                                            <  SRR19973061.1396335/150‑1 (MQ=60)
                                        CGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATC                                                                                                          >  SRR19973061.2597013/1‑150 (MQ=60)
                                        CGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATC                                                                                                          <  SRR19973061.1727982/150‑1 (MQ=60)
                                        CGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATC                                                                                                          >  SRR19973061.3061477/1‑150 (MQ=60)
                                         GTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCA                                                                                                         <  SRR19973061.3647001/150‑1 (MQ=60)
                                            CACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGC                                                                                                      <  SRR19973061.2806461/150‑1 (MQ=60)
                                            CACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGC                                                                                                      <  SRR19973061.1170757/150‑1 (MQ=60)
                                             ACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCG                                                                                                     >  SRR19973061.2081764/1‑150 (MQ=60)
                                               CGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCTCCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAA                                                                                                   <  SRR19973061.1327505/150‑1 (MQ=60)
                                               CGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAA                                                                                                   >  SRR19973061.3628778/1‑150 (MQ=60)
                                               CGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAA                                                                                                   >  SRR19973061.2760437/1‑150 (MQ=60)
                                                 ACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATG                                                                                                 <  SRR19973061.3326984/150‑1 (MQ=60)
                                                  CCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGT                                                                                                <  SRR19973061.3023998/150‑1 (MQ=60)
                                                  CCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGT                                                                                                >  SRR19973061.2242629/1‑150 (MQ=60)
                                                  CCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGT                                                                                                >  SRR19973061.330537/1‑150 (MQ=60)
                                                  CCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGT                                                                                                >  SRR19973061.2242623/1‑150 (MQ=60)
                                                    CCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCG                                                                                              <  SRR19973061.743757/150‑1 (MQ=60)
                                                    CCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCG                                                                                              <  SRR19973061.899384/150‑1 (MQ=60)
                                                    CCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCG                                                                                              <  SRR19973061.2654160/150‑1 (MQ=60)
                                                    CCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCG                                                                                              <  SRR19973061.2650665/150‑1 (MQ=60)
                                                    CCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCG                                                                                              >  SRR19973061.2031434/1‑150 (MQ=60)
                                                      AGTTGCTTCGCCTGCACTGGTGTTGCTCAACATAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGAC                                                                                            <  SRR19973061.3694462/150‑1 (MQ=60)
                                                          GCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCC                                                                                        >  SRR19973061.2281221/1‑150 (MQ=60)
                                                          GCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCC                                                                                        >  SRR19973061.2282526/1‑150 (MQ=60)
                                                           CTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCA                                                                                       >  SRR19973061.1377228/1‑150 (MQ=60)
                                                             TCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACT                                                                                     >  SRR19973061.2759530/1‑150 (MQ=60)
                                                                CCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTA                                                                                  >  SRR19973061.2157799/1‑150 (MQ=60)
                                                                CCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTA                                                                                  >  SRR19973061.3542733/1‑150 (MQ=60)
                                                                 CTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAT                                                                                 <  SRR19973061.1936469/150‑1 (MQ=60)
                                                                 CTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAT                                                                                 >  SRR19973061.2999682/1‑150 (MQ=60)
                                                                 CTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAT                                                                                 >  SRR19973061.2999316/1‑150 (MQ=60)
                                                                  TGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATT                                                                                <  SRR19973061.879048/150‑1 (MQ=60)
                                                                      CTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTA                                                                            >  SRR19973061.2608096/1‑150 (MQ=60)
                                                                      CTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTA                                                                            >  SRR19973061.2470278/1‑150 (MQ=60)
                                                                        GGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATG                                                                          >  SRR19973061.1509264/1‑150 (MQ=60)
                                                                        GGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATG                                                                          >  SRR19973061.2405657/1‑150 (MQ=60)
                                                                        GGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATG                                                                          >  SRR19973061.2405662/1‑150 (MQ=60)
                                                                           GTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATG                                                                       <  SRR19973061.3246248/150‑1 (MQ=60)
                                                                           GTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTAGACGGCCACTTTATTGCTATGATG                                                                       >  SRR19973061.1988273/1‑150 (MQ=60)
                                                                            TTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGC                                                                      >  SRR19973061.3486872/1‑150 (MQ=60)
                                                                              GCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTC                                                                    >  SRR19973061.3193844/1‑150 (MQ=60)
                                                                               CTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCC                                                                   >  SRR19973061.53869/1‑150 (MQ=60)
                                                                                    ttaagtcggagctctAGTTGTCTTAGGCGTGCTATTGTAGGTTTCGCCATCTTTGGTTACAGGCATTCCGCTACCGTTGTCAGATGCAGGCAGATGACAGTTGATCTAGCGTTTGTCGAGGGCCACTTTATTGCTATGATGCTCCCGGTT                                                              <  SRR19973061.1227587/135‑1 (MQ=60)
                                                                                     AAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTT                                                             <  SRR19973061.1314652/150‑1 (MQ=60)
                                                                                       AGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTAT                                                           <  SRR19973061.210408/150‑1 (MQ=60)
                                                                                          CGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTctagg                                                        >  SRR19973061.1427283/1‑145 (MQ=60)
                                                                                               CACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTG                                                   >  SRR19973061.1194319/1‑150 (MQ=60)
                                                                                                   AGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGT                                               >  SRR19973061.2901257/1‑150 (MQ=60)
                                                                                                   AGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGT                                               >  SRR19973061.1926062/1‑150 (MQ=60)
                                                                                                      TGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGAC                                            >  SRR19973061.2553961/1‑150 (MQ=60)
                                                                                                       GTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACT                                           <  SRR19973061.2945002/150‑1 (MQ=60)
                                                                                                         CTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTG                                         >  SRR19973061.2069694/1‑150 (MQ=60)
                                                                                                          TTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGT                                        <  SRR19973061.1870315/150‑1 (MQ=60)
                                                                                                           TAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTC                                       >  SRR19973061.1724186/1‑150 (MQ=60)
                                                                                                             GCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCA                                     <  SRR19973061.600419/150‑1 (MQ=60)
                                                                                                              CCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAA                                    <  SRR19973061.744916/150‑1 (MQ=60)
                                                                                                                 TGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGAT                                 <  SRR19973061.3710127/150‑1 (MQ=60)
                                                                                                                   CTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCT                               <  SRR19973061.2397952/150‑1 (MQ=60)
                                                                                                                   CTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCT                               <  SRR19973061.868673/150‑1 (MQ=60)
                                                                                                                     ATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTAT                             <  SRR19973061.543760/150‑1 (MQ=60)
                                                                                                                        GAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTT                          <  SRR19973061.1373283/150‑1 (MQ=60)
                                                                                                                          AGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTT                        <  SRR19973061.532205/150‑1 (MQ=60)
                                                                                                                          AGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTT                        >  SRR19973061.1294618/1‑150 (MQ=60)
                                                                                                                            GTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTA                      <  SRR19973061.527840/150‑1 (MQ=60)
                                                                                                                                   CAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAT               <  SRR19973061.3303106/150‑1 (MQ=60)
                                                                                                                                   CAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAT               <  SRR19973061.3482532/150‑1 (MQ=60)
                                                                                                                                    AACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATC              <  SRR19973061.1847449/150‑1 (MQ=60)
                                                                                                                                    AACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATC              <  SRR19973061.1434381/150‑1 (MQ=60)
                                                                                                                                     ACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCT             <  SRR19973061.288671/150‑1 (MQ=60)
                                                                                                                                      CTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTT            <  SRR19973061.1809527/150‑1 (MQ=60)
                                                                                                                                       TTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTC           <  SRR19973061.2637339/150‑1 (MQ=60)
                                                                                                                                       TTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTC           <  SRR19973061.2638355/150‑1 (MQ=60)
                                                                                                                                        TTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCT          >  SRR19973061.1509712/1‑150 (MQ=60)
                                                                                                                                        TTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCT          <  SRR19973061.586730/150‑1 (MQ=60)
                                                                                                                                        TTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCT          <  SRR19973061.2031031/150‑1 (MQ=60)
                                                                                                                                          GGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGG        <  SRR19973061.2515301/150‑1 (MQ=60)
                                                                                                                                           GATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGA       >  SRR19973061.378641/1‑150 (MQ=60)
                                                                                                                                            ATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGAT      <  SRR19973061.194165/150‑1 (MQ=60)
                                                                                                                                               CAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAA   >  SRR19973061.609515/1‑150 (MQ=60)
                                                                                                                                                AGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAAT  >  SRR19973061.783848/1‑150 (MQ=60)
                                                                                                                                                AGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAAT  <  SRR19973061.826486/150‑1 (MQ=60)
                                                                                                                                                |                                                                                                                                                     
TGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAAT  >  CP009273/747829‑748122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑