Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 747,973 C→A 100% P93P (CCG→CCT)  ybgD ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273747,9730CA97.5% 754.3 / 8.8 242P93P (CCG→CCT) ybgDputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base A (109/127);  total (113/129)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 242 total aligned reads displayed.

TGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGA  >  CP009273/747829‑748117
                                                                                                                                                |                                                                                                                                                
 gAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCa                                                                                                                                              <  2:2416920/148‑1 (MQ=255)
 gAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCa                                                                                                                                              <  2:2102207/148‑1 (MQ=255)
  aaCCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAt                                                                                                                                             >  1:2880953/1‑148 (MQ=255)
  aaCCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAt                                                                                                                                             <  1:1876895/148‑1 (MQ=255)
   aCCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAtt                                                                                                                                            <  1:445374/148‑1 (MQ=255)
    ccTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTc                                                                                                                                           >  1:2612319/1‑148 (MQ=255)
    ccTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTc                                                                                                                                           <  2:618795/148‑1 (MQ=255)
       aaTACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGc                                                                                                                                        >  1:3098713/1‑148 (MQ=255)
       aaTACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGc                                                                                                                                        >  1:467675/1‑148 (MQ=255)
        aTACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCt                                                                                                                                       >  1:2530936/1‑148 (MQ=255)
         tACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTa                                                                                                                                      <  1:1021950/148‑1 (MQ=255)
          aCGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTAc                                                                                                                                     >  1:1244437/1‑148 (MQ=255)
               gTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTg                                                                                                                                >  2:1477046/1‑148 (MQ=255)
               gTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTg                                                                                                                                >  2:1605285/1‑148 (MQ=255)
               gTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTg                                                                                                                                <  2:2574307/148‑1 (MQ=255)
                ttACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGt                                                                                                                               >  1:204397/1‑148 (MQ=255)
                    cGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGa                                                                                                                           <  1:3479400/148‑1 (MQ=255)
                         tttttGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcag                                                                                                                      >  1:1479956/1‑148 (MQ=255)
                         tttttGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcag                                                                                                                      >  1:356883/1‑148 (MQ=255)
                         tttttGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcag                                                                                                                      >  1:1997392/1‑148 (MQ=255)
                         tttttGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcag                                                                                                                      >  1:2135506/1‑148 (MQ=255)
                          ttttGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcagg                                                                                                                     >  1:376454/1‑148 (MQ=255)
                          ttttGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcagg                                                                                                                     >  2:1715242/1‑148 (MQ=255)
                          ttttGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcagg                                                                                                                     >  2:876235/1‑148 (MQ=255)
                            ttGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcaggca                                                                                                                   >  2:2647317/1‑148 (MQ=255)
                               tCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGAt                                                                                                                <  2:2608760/148‑1 (MQ=255)
                                ccATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATc                                                                                                               >  1:1117636/1‑148 (MQ=255)
                                ccATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATc                                                                                                               >  2:3574593/1‑148 (MQ=255)
                                  aTCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATcac                                                                                                             <  2:1676776/148‑1 (MQ=255)
                                   tCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATcaca                                                                                                            <  1:3676199/148‑1 (MQ=255)
                                   tCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATcaca                                                                                                            >  2:378251/1‑148 (MQ=255)
                                     aGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGt                                                                                                          >  1:3273279/1‑148 (MQ=255)
                                      gTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGtt                                                                                                         >  1:676524/1‑148 (MQ=255)
                                       tCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTg                                                                                                        >  2:1781083/1‑148 (MQ=255)
                                       tCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTg                                                                                                        >  2:6861/1‑148 (MQ=255)
                                        cGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGa                                                                                                       <  1:2570316/148‑1 (MQ=255)
                                           acacCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCa                                                                                                    <  2:3466579/148‑1 (MQ=255)
                                           acacCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCa                                                                                                    <  2:1634287/148‑1 (MQ=255)
                                             acCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCacg                                                                                                  <  1:901190/148‑3 (MQ=255)
                                                  ccccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAAt                                                                                             <  2:1140341/148‑1 (MQ=255)
                                                    ccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGt                                                                                           <  1:1020774/148‑1 (MQ=255)
                                                    ccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGt                                                                                           >  1:2360590/1‑148 (MQ=255)
                                                      aGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCg                                                                                         <  1:1905861/148‑1 (MQ=255)
                                                       gTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGa                                                                                        >  1:2316090/1‑148 (MQ=255)
                                                            ttCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGcc                                                                                   >  2:1773528/1‑148 (MQ=255)
                                                             tCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCa                                                                                  <  2:3129520/148‑1 (MQ=255)
                                                             tCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCa                                                                                  >  2:3581765/1‑148 (MQ=255)
                                                                ccTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACtt                                                                               <  1:1690234/148‑1 (MQ=255)
                                                                   gCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAt                                                                            <  2:3663058/148‑1 (MQ=255)
                                                                    cACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAtt                                                                           >  1:1344203/1‑148 (MQ=255)
                                                                       tGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTAACGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTTCt                                                                        >  2:498298/1‑148 (MQ=255)
                                                                        ggTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTa                                                                       >  2:447029/1‑148 (MQ=255)
                                                                          tgtTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTatg                                                                     >  2:533805/1‑148 (MQ=255)
                                                                           gtTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTatga                                                                    >  2:3725295/1‑148 (MQ=255)
                                                                               cTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCt                                                                <  1:3794787/148‑1 (MQ=255)
                                                                                 caacaaAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTcc                                                              <  2:3635144/148‑1 (MQ=255)
                                                                                 caacaaAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTcc                                                              <  2:28017/148‑1 (MQ=255)
                                                                                    caaAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCgg                                                           <  2:1405952/148‑1 (MQ=255)
                                                                                         gCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTtat                                                      <  2:1499655/148‑1 (MQ=255)
                                                                                         gCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTtat                                                      <  2:1500155/148‑1 (MQ=255)
                                                                                         gCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTtat                                                      <  2:288111/148‑1 (MQ=255)
                                                                                          cGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTtata                                                     <  2:1885041/148‑1 (MQ=255)
                                                                                          cGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTtata                                                     <  2:2639648/148‑1 (MQ=255)
                                                                                              gCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATggg                                                 <  1:1800117/148‑1 (MQ=255)
                                                                                               cACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGACACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGt                                                <  1:548585/148‑1 (MQ=255)
                                                                                                 ccAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTg                                              <  1:2457726/148‑1 (MQ=255)
                                                                                                  cAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGt                                             <  2:2286596/148‑1 (MQ=255)
                                                                                                     ttGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGt                                          >  2:1251198/1‑148 (MQ=255)
                                                                                                             gCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTc                                  <  2:370881/148‑1 (MQ=255)
                                                                                                             gCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTc                                  <  2:3263740/148‑1 (MQ=255)
                                                                                                              ccGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTcc                                 >  2:305085/1‑148 (MQ=255)
                                                                                                                 tGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAg                              >  1:2833104/1‑148 (MQ=255)
                                                                                                                 tGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAg                              >  1:30276/1‑148 (MQ=255)
                                                                                                                   cTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGAt                            <  1:2654272/148‑1 (MQ=255)
                                                                                                                   cTATCGAAGGTTACGCCAACTTTGGATACAGGCATTACGCTAGCGTTGTCAGTAGCAGGCAGATCAGAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGAt                            <  1:708552/148‑1 (MQ=255)
                                                                                                                     aTCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCt                          >  2:2069825/1‑148 (MQ=255)
                                                                                                                      tCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTa                         >  2:3755332/1‑148 (MQ=255)
                                                                                                                        gAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATg                       <  2:2471028/148‑1 (MQ=255)
                                                                                                                        gAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATg                       <  1:2263710/148‑1 (MQ=255)
                                                                                                                        gAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATg                       <  1:1337013/148‑1 (MQ=255)
                                                                                                                         aaGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGt                      <  2:1244437/148‑1 (MQ=255)
                                                                                                                          aGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGtt                     <  1:3459551/148‑1 (MQ=255)
                                                                                                                           ggTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGttt                    >  2:575440/1‑148 (MQ=255)
                                                                                                                           ggTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGttt                    >  1:3356515/1‑148 (MQ=255)
                                                                                                                           ggTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGttt                    <  2:2235825/148‑1 (MQ=255)
                                                                                                                              tACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTa                 >  2:2674600/1‑148 (MQ=255)
                                                                                                                              tACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTa                 >  1:1855239/1‑148 (MQ=255)
                                                                                                                               aCGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTAt                <  1:3522441/148‑1 (MQ=255)
                                                                                                                                  ccAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCaa             >  1:2804694/1‑148 (MQ=255)
                                                                                                                                   cAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAat            >  1:1523712/1‑148 (MQ=255)
                                                                                                                                   cAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAat            >  1:1158580/1‑148 (MQ=255)
                                                                                                                                     aCTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAatat          <  1:2380285/148‑1 (MQ=255)
                                                                                                                                     aCTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAatat          >  1:1805105/1‑148 (MQ=255)
                                                                                                                                     aCTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAatat          <  2:2036634/148‑1 (MQ=255)
                                                                                                                                      cTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtc         <  2:360520/148‑1 (MQ=255)
                                                                                                                                      cTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtc         >  1:1404402/1‑148 (MQ=255)
                                                                                                                                      cTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtc         <  2:2296734/148‑1 (MQ=255)
                                                                                                                                          ggATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtcttct     <  2:1314309/148‑1 (MQ=255)
                                                                                                                                          ggATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtcttct     <  1:1645986/148‑1 (MQ=255)
                                                                                                                                            aTACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTgg   <  2:1897837/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGA  >  CP009273/747829‑748117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAAT  >  CP009273/747825‑748122
                                                                                                                                                    |                                                                                                                                                     
CGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAG                                                                                                                                                      >  SRR19973069.669245/1‑150 (MQ=60)
 GGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGG                                                                                                                                                     <  SRR19973069.259756/150‑1 (MQ=60)
 GGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGG                                                                                                                                                     <  SRR19973069.258301/150‑1 (MQ=60)
  GCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGC                                                                                                                                                    <  SRR19973069.236912/150‑1 (MQ=60)
    TGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAT                                                                                                                                                  >  SRR19973069.2899877/1‑150 (MQ=60)
     GAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATT                                                                                                                                                 <  SRR19973069.2492328/150‑1 (MQ=60)
      AACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTC                                                                                                                                                >  SRR19973069.2629784/1‑150 (MQ=60)
      AACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTC                                                                                                                                                <  SRR19973069.1889944/150‑1 (MQ=60)
       ACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCC                                                                                                                                               <  SRR19973069.448629/150‑1 (MQ=60)
       ACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCC                                                                                                                                               <  SRR19973069.448550/150‑1 (MQ=60)
         CTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGC                                                                                                                                             >  SRR19973069.471088/1‑150 (MQ=60)
         CTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGC                                                                                                                                             >  SRR19973069.3119129/1‑150 (MQ=60)
         CTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGC                                                                                                                                             >  SRR19973069.3119128/1‑150 (MQ=60)
         CTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGC                                                                                                                                             >  SRR19973069.3119070/1‑150 (MQ=60)
          TAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCT                                                                                                                                            >  SRR19973069.2547721/1‑150 (MQ=60)
            ATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTAC                                                                                                                                          >  SRR19973069.1480316/1‑150 (MQ=60)
            ATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTAC                                                                                                                                          >  SRR19973069.2752239/1‑150 (MQ=60)
            ATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTAC                                                                                                                                          >  SRR19973069.1252887/1‑150 (MQ=60)
             TACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACC                                                                                                                                         <  SRR19973069.1029021/150‑1 (MQ=60)
                GATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTT                                                                                                                                      <  SRR19973069.1330466/150‑1 (MQ=60)
                GATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTT                                                                                                                                      <  SRR19973069.2549631/150‑1 (MQ=60)
                  TGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGT                                                                                                                                    >  SRR19973069.206032/1‑150 (MQ=60)
                        CGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAG                                                                                                                              <  SRR19973069.3501875/150‑1 (MQ=60)
                        CGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAG                                                                                                                              <  SRR19973069.3501881/150‑1 (MQ=60)
                        CGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAG                                                                                                                              <  SRR19973069.3501941/150‑1 (MQ=60)
                           CATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAG                                                                                                                           >  SRR19973069.359439/1‑150 (MQ=60)
                           CATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAG                                                                                                                           >  SRR19973069.1490252/1‑150 (MQ=60)
                           CATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAG                                                                                                                           >  SRR19973069.2150181/1‑150 (MQ=60)
                           CATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAG                                                                                                                           >  SRR19973069.2011307/1‑150 (MQ=60)
                           CATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGATCAACAAAGTCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGAAACAGGCATTCCGCTACCGTTGTCAGAAGCAG                                                                                                                           >  SRR19973069.2011104/1‑150 (MQ=60)
                            ATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGG                                                                                                                          >  SRR19973069.379077/1‑150 (MQ=60)
                            ATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGG                                                                                                                          >  SRR19973069.379202/1‑150 (MQ=60)
                                  GTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATC                                                                                                                    >  SRR19973069.1125431/1‑150 (MQ=60)
                                   TCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCA                                                                                                                   <  SRR19973069.2415014/150‑1 (MQ=60)
                                       TCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGT                                                                                                               >  SRR19973069.3294712/1‑150 (MQ=60)
                                       TCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGT                                                                                                               <  SRR19973069.3700246/150‑1 (MQ=60)
                                       TCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGT                                                                                                               <  SRR19973069.3700256/150‑1 (MQ=60)
                                       TCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGT                                                                                                               >  SRR19973069.3830519/1‑150 (MQ=60)
                                        CAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTT                                                                                                              >  SRR19973069.681204/1‑150 (MQ=60)
                                         AGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTG                                                                                                             >  SRR19973069.1675391/1‑150 (MQ=60)
                                            CGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATC                                                                                                          <  SRR19973069.2587341/150‑1 (MQ=60)
                                                 ACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCACGCG                                                                                                     <  SRR19973069.907386/150‑1 (MQ=60)
                                                    GACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAAT                                                                                                  <  SRR19973069.1880245/150‑1 (MQ=60)
                                                     ACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATG                                                                                                 <  SRR19973069.3391764/150‑1 (MQ=60)
                                                      CCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGT                                                                                                >  SRR19973069.2376646/1‑150 (MQ=60)
                                                      CCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGT                                                                                                >  SRR19973069.2376631/1‑150 (MQ=60)
                                                        CCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCG                                                                                              <  SRR19973069.1027837/150‑1 (MQ=60)
                                                        CCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCG                                                                                              <  SRR19973069.1028674/150‑1 (MQ=60)
                                                         CAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGA                                                                                             >  SRR19973069.2331855/1‑150 (MQ=60)
                                                          AGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGAC                                                                                            <  SRR19973069.1919023/150‑1 (MQ=60)
                                                          AGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGAC                                                                                            >  SRR19973069.748098/1‑150 (MQ=60)
                                                              GCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCC                                                                                        >  SRR19973069.1030963/1‑150 (MQ=60)
                                                               CTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCA                                                                                       >  SRR19973069.1942704/1‑150 (MQ=60)
                                                               CTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCA                                                                                       >  SRR19973069.2185569/1‑150 (MQ=60)
                                                                 TCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACT                                                                                     <  SRR19973069.1482456/150‑1 (MQ=60)
                                                                    CCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTA                                                                                  <  SRR19973069.1702037/150‑1 (MQ=60)
                                                                     CTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAT                                                                                 <  SRR19973069.381012/150‑1 (MQ=60)
                                                                     CTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAT                                                                                 >  SRR19973069.2895467/1‑150 (MQ=60)
                                                                      TGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATT                                                                                <  SRR19973069.2362894/150‑1 (MQ=60)
                                                                      TGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATT                                                                                >  SRR19973069.1353506/1‑150 (MQ=60)
                                                                      TGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATT                                                                                >  SRR19973069.1354033/1‑150 (MQ=60)
                                                                      TGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATT                                                                                >  SRR19973069.1352959/1‑150 (MQ=60)
                                                                           TGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTAT                                                                           >  SRR19973069.1158838/1‑150 (MQ=60)
                                                                              TGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGAT                                                                        <  SRR19973069.2353732/150‑1 (MQ=60)
                                                                               GTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATG                                                                       <  SRR19973069.2446265/150‑1 (MQ=60)
                                                                                   CTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCC                                                                   <  SRR19973069.3819382/150‑1 (MQ=60)
                                                                                     CAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAAATTTGGAAACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCG                                                                 >  SRR19973069.2048690/1‑150 (MQ=60)
                                                                                       ACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGT                                                               >  SRR19973069.867820/1‑150 (MQ=60)
                                                                                         AAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTT                                                             >  SRR19973069.468435/1‑150 (MQ=60)
                                                                                         AAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTT                                                             <  SRR19973069.1901525/150‑1 (MQ=60)
                                                                                                  GCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTT                                                    <  SRR19973069.1812793/150‑1 (MQ=60)
                                                                                                   CACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTG                                                   >  SRR19973069.2604789/1‑150 (MQ=60)
                                                                                                   CACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTG                                                   >  SRR19973069.2604809/1‑150 (MQ=60)
                                                                                                   CACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTG                                                   <  SRR19973069.553303/150‑1 (MQ=60)
                                                                                                   CACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGACACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTG                                                   <  SRR19973069.552426/150‑1 (MQ=60)
                                                                                                     CCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTC                                                 <  SRR19973069.2474240/150‑1 (MQ=60)
                                                                                                     CCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTC                                                 <  SRR19973069.3334130/150‑1 (MQ=60)
                                                                                                     CCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTC                                                 >  SRR19973069.2183454/1‑150 (MQ=60)
                                                                                                      CAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCG                                                <  SRR19973069.1834060/150‑1 (MQ=60)
                                                                                                      CAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCG                                                <  SRR19973069.1560116/150‑1 (MQ=60)
                                                                                                       AGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGACTGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGT                                               >  SRR19973069.2069586/1‑150 (MQ=60)
                                                                                                       AGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGT                                               >  SRR19973069.3583973/1‑150 (MQ=60)
                                                                                                       AGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGT                                               >  SRR19973069.3584084/1‑150 (MQ=60)
                                                                                                             CTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTG                                         >  SRR19973069.2949312/1‑150 (MQ=60)
                                                                                                                   CGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAG                                   >  SRR19973069.30706/1‑150 (MQ=60)
                                                                                                                   CGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAG                                   >  SRR19973069.2853829/1‑150 (MQ=60)
                                                                                                                   CGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAG                                   >  SRR19973069.2851815/1‑150 (MQ=60)
                                                                                                                   CGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAG                                   <  SRR19973069.6972/150‑1 (MQ=60)
                                                                                                                       CTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCT                               <  SRR19973069.2313749/150‑1 (MQ=60)
                                                                                                                       CTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCT                               <  SRR19973069.2672122/150‑1 (MQ=60)
                                                                                                                       CTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCT                               <  SRR19973069.2672061/150‑1 (MQ=60)
                                                                                                                       CTATCGAAGGTTACGCCAACTTTGGATACAGGCATTACGCTAGCGTTGTCAGTAGCAGGCAGATCAGAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCT                               <  SRR19973069.713528/150‑1 (MQ=60)
                                                                                                                        TATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTA                              <  SRR19973069.3177426/150‑1 (MQ=60)
                                                                                                                        TATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTA                              <  SRR19973069.60597/150‑1 (MQ=60)
                                                                                                                          TCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATG                            <  SRR19973069.1315330/150‑1 (MQ=60)
                                                                                                                           CGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGT                           >  SRR19973069.3678138/1‑150 (MQ=60)
                                                                                                                           CGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGT                           >  SRR19973069.1774068/1‑150 (MQ=60)
                                                                                                                            GAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTT                          <  SRR19973069.2279043/150‑1 (MQ=60)
                                                                                                                            GAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTT                          <  SRR19973069.1346240/150‑1 (MQ=60)
                                                                                                                            GAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTT                          <  SRR19973069.1816882/150‑1 (MQ=60)
                                                                                                                             AAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTT                         >  SRR19973069.3378477/1‑150 (MQ=60)
                                                                                                                              AGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTT                        <  SRR19973069.3481948/150‑1 (MQ=60)
                                                                                                                                GTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTA                      >  SRR19973069.1868185/1‑150 (MQ=60)
                                                                                                                                   ACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCA                   <  SRR19973069.3545204/150‑1 (MQ=60)
                                                                                                                                    CGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAA                  >  SRR19973069.2823331/1‑150 (MQ=60)
                                                                                                                                    CGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAA                  >  SRR19973069.2823302/1‑150 (MQ=60)
                                                                                                                                    CGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAA                  <  SRR19973069.189158/150‑1 (MQ=60)
                                                                                                                                     GCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAAT                 >  SRR19973069.1534209/1‑150 (MQ=60)
                                                                                                                                     GCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAAT                 >  SRR19973069.1166206/1‑150 (MQ=60)
                                                                                                                                     GCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAAT                 >  SRR19973069.1166647/1‑150 (MQ=60)
                                                                                                                                     GCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAAT                 >  SRR19973069.1169129/1‑150 (MQ=60)
                                                                                                                                     GCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATAAAT                 >  SRR19973069.1533871/1‑150 (MQ=60)
                                                                                                                                       CAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAT               >  SRR19973069.1817801/1‑150 (MQ=60)
                                                                                                                                        AACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATC              >  SRR19973069.2660990/1‑150 (MQ=60)
                                                                                                                                        AACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATC              >  SRR19973069.1414200/1‑150 (MQ=60)
                                                                                                                                         ACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCT             <  SRR19973069.2396429/150‑1 (MQ=60)
                                                                                                                                         ACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCT             >  SRR19973069.346942/1‑150 (MQ=60)
                                                                                                                                          CTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTT            <  SRR19973069.1722084/150‑1 (MQ=60)
                                                                                                                                            TTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCT          <  SRR19973069.1567718/150‑1 (MQ=60)
                                                                                                                                              GGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGG        <  SRR19973069.1657331/150‑1 (MQ=60)
                                                                                                                                                ATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGAT      >  SRR19973069.1080589/1‑150 (MQ=60)
                                                                                                                                                ATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGAT      >  SRR19973069.1082327/1‑150 (MQ=60)
                                                                                                                                                 TACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATG     <  SRR19973069.51220/150‑1 (MQ=60)
                                                                                                                                                  ACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGA    >  SRR19973069.644382/1‑150 (MQ=60)
                                                                                                                                                  ACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGA    >  SRR19973069.492574/1‑150 (MQ=60)
                                                                                                                                                   CAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAA   >  SRR19973069.2159007/1‑150 (MQ=60)
                                                                                                                                                   CAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAA   >  SRR19973069.215958/1‑150 (MQ=60)
                                                                                                                                                    AGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAAT  >  SRR19973069.525192/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAAT  >  CP009273/747825‑748122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑