Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 747,973 C→A 100% P93P (CCG→CCT)  ybgD ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273747,9730CA98.7% 731.2 / ‑0.6 223P93P (CCG→CCT) ybgDputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (108/111);  total (109/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

GGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGG  >  CP009273/747826‑748116
                                                                                                                                                   |                                                                                                                                               
ggCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAgg                                                                                                                                               >  1:419515/1‑150 (MQ=255)
  cTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCa                                                                                                                                             <  1:5066704/150‑1 (MQ=255)
  cTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGAAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCa                                                                                                                                             >  1:1702322/1‑150 (MQ=255)
   tGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAt                                                                                                                                            <  1:4222686/150‑1 (MQ=255)
     aaCCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTc                                                                                                                                          <  1:4465801/150‑1 (MQ=255)
     aaCCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGAGCAATCGAAGGTTACGCCAACTTTGGATACAGGCATTc                                                                                                                                          >  2:3671553/1‑150 (MQ=255)
      aCCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTcc                                                                                                                                         <  2:4415218/150‑1 (MQ=255)
          aaTACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTa                                                                                                                                     <  2:2641229/150‑1 (MQ=255)
               gATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGtt                                                                                                                                <  1:449677/150‑1 (MQ=255)
               gATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGtt                                                                                                                                <  2:4174329/150‑1 (MQ=255)
                 tGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGt                                                                                                                              >  1:2540048/1‑150 (MQ=255)
                    tACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAg                                                                                                                           <  2:2211501/150‑1 (MQ=255)
                     aCCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGa                                                                                                                          <  1:4595495/150‑1 (MQ=255)
                      ccttCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGAACCAGTTGTATTATCAGTGCTATAGAAGGTTACTAAAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGaa                                                                                                                         <  1:3806990/147‑1 (MQ=255)
                       tgTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAg                                                                                                                        >  2:2792544/2‑150 (MQ=255)
                          cATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcag                                                                                                                     <  1:4793614/150‑1 (MQ=255)
                            tttttGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcaggc                                                                                                                   >  1:604528/1‑150 (MQ=255)
                             gtttGTCCATCCGTCGTACACCGACCCCCGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcaggca                                                                                                                  <  1:3417920/149‑1 (MQ=255)
                              tttGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAgcaggcag                                                                                                                 <  1:2203482/150‑1 (MQ=255)
                                tGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGAt                                                                                                               >  1:2726719/1‑150 (MQ=255)
                                tGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGAt                                                                                                               >  2:1509096/1‑150 (MQ=255)
                                 gTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATc                                                                                                              >  1:2090044/1‑150 (MQ=255)
                                 gTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATc                                                                                                              <  2:4046073/150‑1 (MQ=255)
                                  tCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATca                                                                                                             >  2:2201963/1‑150 (MQ=255)
                                  tCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATca                                                                                                             <  1:4868065/150‑1 (MQ=255)
                                     aTCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAg                                                                                                          >  1:3808167/1‑150 (MQ=255)
                                      tCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGt                                                                                                         >  1:177408/1‑150 (MQ=255)
                                        aGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTg                                                                                                       <  1:400021/150‑1 (MQ=255)
                                         gTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGa                                                                                                      <  1:5053739/150‑1 (MQ=255)
                                         gTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGa                                                                                                      >  1:2761787/1‑150 (MQ=255)
                                            gTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCa                                                                                                   >  2:1331652/1‑150 (MQ=255)
                                            gTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCa                                                                                                   <  1:1312442/150‑1 (MQ=255)
                                             tACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCaa                                                                                                  <  2:4248654/150‑1 (MQ=255)
                                               cacCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGc                                                                                                >  1:3644884/1‑150 (MQ=255)
                                                acCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCg                                                                                               >  2:2072864/1‑150 (MQ=255)
                                                 ccGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGa                                                                                              <  1:4606258/150‑1 (MQ=255)
                                                  cGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGaa                                                                                             >  2:3119068/1‑150 (MQ=255)
                                                  cGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGGTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGaa                                                                                             >  1:2385061/1‑150 (MQ=255)
                                                   gACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGact                                                                                            >  2:4923502/1‑148 (MQ=255)
                                                    aCCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATg                                                                                           <  2:1065067/150‑1 (MQ=255)
                                                    aCCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATg                                                                                           >  1:548544/1‑150 (MQ=255)
                                                      cccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTc                                                                                         <  1:2984409/150‑1 (MQ=255)
                                                       ccAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCg                                                                                        >  2:2445701/1‑150 (MQ=255)
                                                         aGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGAc                                                                                      <  2:4604243/150‑1 (MQ=255)
                                                          gTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACg                                                                                     <  2:2408267/150‑1 (MQ=255)
                                                              cTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCa                                                                                 >  1:3392895/1‑150 (MQ=255)
                                                                     tGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTAtt                                                                          <  1:1079653/150‑1 (MQ=255)
                                                                        accggTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCt                                                                       <  2:567699/147‑1 (MQ=255)
                                                                        aCTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATAAAGAGAATGTCGACGGCCACTTTATTGCt                                                                       <  1:2041026/150‑1 (MQ=255)
                                                                           ggTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTatg                                                                    >  2:2014958/1‑150 (MQ=255)
                                                                             tgtTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTatgat                                                                  >  1:2594194/1‑150 (MQ=255)
                                                                               ttGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGc                                                                >  2:4467890/1‑150 (MQ=255)
                                                                                tGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCt                                                               <  1:1461421/150‑1 (MQ=255)
                                                                                  cTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTcc                                                             >  2:4253078/1‑150 (MQ=255)
                                                                                   tCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTcac                                                            >  2:3561650/1‑148 (MQ=255)
                                                                                     aacaaAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTccctg                                                          >  1:2372352/1‑148 (MQ=255)
                                                                                      acaaAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGt                                                         >  1:1137759/1‑150 (MQ=255)
                                                                                       caaAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGtt                                                        <  1:977241/150‑1 (MQ=255)
                                                                                        aaaGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGttt                                                       >  2:1277487/1‑150 (MQ=255)
                                                                                         aaGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTta                                                      >  2:1247087/1‑150 (MQ=255)
                                                                                         aaGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTta                                                      >  2:4792608/1‑150 (MQ=255)
                                                                                          aGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTtnt                                                     <  1:3900313/150‑1 (MQ=255)
                                                                                          aGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTtat                                                     >  2:4758629/1‑150 (MQ=255)
                                                                                             cGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATg                                                  >  2:1690263/1‑150 (MQ=255)
                                                                                             cGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATg                                                  >  1:688417/1‑150 (MQ=255)
                                                                                             cGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATg                                                  >  1:2079195/1‑150 (MQ=255)
                                                                                              gTAGCACCAGTTGTCTTAGGCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATgg                                                 >  2:1000435/1‑150 (MQ=255)
                                                                                              gTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATgg                                                 >  1:435212/1‑150 (MQ=255)
                                                                                               tAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATggg                                                <  1:1904605/150‑1 (MQ=255)
                                                                                                 gCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGtt                                              <  2:2909419/150‑1 (MQ=255)
                                                                                                  cACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATAGCTATGATGCTCCCGGTTTATATGGGTTg                                             >  2:532325/1‑150 (MQ=255)
                                                                                                   aCCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGt                                            >  1:2122338/1‑150 (MQ=255)
                                                                                                      aGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGt                                         >  1:4903062/1‑150 (MQ=255)
                                                                                                             ttAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGt                                  <  2:2753838/150‑1 (MQ=255)
                                                                                                                gCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCa                               <  1:3717830/150‑1 (MQ=255)
                                                                                                                  cGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAg                             <  2:2540048/150‑1 (MQ=255)
                                                                                                                    tGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTCTTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGAt                           >  1:4826980/1‑150 (MQ=255)
                                                                                                                    tGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGAt                           >  1:895602/1‑150 (MQ=255)
                                                                                                                      cTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCt                         <  2:175178/150‑1 (MQ=255)
                                                                                                                      cTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCt                         <  2:3085490/150‑1 (MQ=255)
                                                                                                                       tATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTa                        >  1:4163379/1‑150 (MQ=255)
                                                                                                                       tATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTa                        <  1:3836234/150‑1 (MQ=255)
                                                                                                                        aTCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTAt                       >  2:1356323/1‑150 (MQ=255)
                                                                                                                        aTCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTAt                       <  2:4551828/150‑1 (MQ=255)
                                                                                                                        aTCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTAt                       <  2:1959874/150‑1 (MQ=255)
                                                                                                                        aTCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTAt                       >  2:2364676/1‑150 (MQ=255)
                                                                                                                         tCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATg                      <  1:4079468/150‑1 (MQ=255)
                                                                                                                           gAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGtt                    <  2:4140078/150‑1 (MQ=255)
                                                                                                                           gAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGtt                    >  1:4426259/1‑150 (MQ=255)
                                                                                                                            aaGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGttt                   <  1:130250/150‑1 (MQ=255)
                                                                                                                            aaGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGttt                   >  1:3577004/1‑150 (MQ=255)
                                                                                                                                 tACGCCAAGTTTGGATACAGGCTGTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATc              <  1:4654241/150‑1 (MQ=255)
                                                                                                                                 tACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATc              >  2:4271227/1‑150 (MQ=255)
                                                                                                                                 tACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATc              >  2:3630750/1‑150 (MQ=255)
                                                                                                                                       aaCTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtc        <  2:4841308/150‑1 (MQ=255)
                                                                                                                                        aCTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtct       <  2:3860636/150‑1 (MQ=255)
                                                                                                                                        aCTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtct       <  1:1251668/150‑1 (MQ=255)
                                                                                                                                          tttGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATAtcttc     <  1:2014958/150‑1 (MQ=255)
                                                                                                                                             ggATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTgg  <  1:1509096/150‑1 (MQ=255)
                                                                                                                                             ggATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTgg  >  2:3293788/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGG  >  CP009273/747826‑748116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGA  >  CP009273/747826‑748120
                                                                                                                                                   |                                                                                                                                                   
GGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGG                                                                                                                                                   >  SRR19973063.420688/1‑150 (MQ=60)
  CTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCA                                                                                                                                                 <  SRR19973063.2516373/150‑1 (MQ=60)
  CTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCA                                                                                                                                                 <  SRR19973063.5083009/150‑1 (MQ=60)
  CTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGAAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCA                                                                                                                                                 >  SRR19973063.1707511/1‑150 (MQ=60)
   TGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAT                                                                                                                                                >  SRR19973063.3797204/1‑150 (MQ=60)
   TGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCAT                                                                                                                                                <  SRR19973063.4236147/150‑1 (MQ=60)
     AACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTC                                                                                                                                              <  SRR19973063.3467971/150‑1 (MQ=60)
     AACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTC                                                                                                                                              <  SRR19973063.4480133/150‑1 (MQ=60)
     AACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTC                                                                                                                                              <  SRR19973063.4128329/150‑1 (MQ=60)
      ACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCC                                                                                                                                             <  SRR19973063.5094253/150‑1 (MQ=60)
         TAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCT                                                                                                                                          <  SRR19973063.1450370/150‑1 (MQ=60)
          AATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTA                                                                                                                                         >  SRR19973063.140705/1‑150 (MQ=60)
               GATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTT                                                                                                                                    <  SRR19973063.4189895/150‑1 (MQ=60)
               GATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTT                                                                                                                                    <  SRR19973063.450942/150‑1 (MQ=60)
               GATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTT                                                                                                                                    >  SRR19973063.755024/1‑150 (MQ=60)
                ATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTG                                                                                                                                   <  SRR19973063.3304779/150‑1 (MQ=60)
                 TGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGT                                                                                                                                  >  SRR19973063.2548149/1‑150 (MQ=60)
                     ACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGA                                                                                                                              <  SRR19973063.4610201/150‑1 (MQ=60)
                     ACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGA                                                                                                                              >  SRR19973063.4856887/1‑150 (MQ=60)
                     ACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGGAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGA                                                                                                                              <  SRR19973063.1860961/150‑1 (MQ=60)
                      CCTTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGAACCAGTTGTATTATCAGTGCTATAGAAGGTTACTAAAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAA                                                                                                                             <  SRR19973063.3819112/150‑1 (MQ=60)
                      CCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAA                                                                                                                             <  SRR19973063.990584/150‑1 (MQ=60)
                      CCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAA                                                                                                                             <  SRR19973063.3819120/150‑1 (MQ=60)
                        GTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGC                                                                                                                           >  SRR19973063.4905684/1‑150 (MQ=60)
                         TCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCA                                                                                                                          <  SRR19973063.2743204/150‑1 (MQ=60)
                          CATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAG                                                                                                                         <  SRR19973063.4808927/150‑1 (MQ=60)
                            TTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGC                                                                                                                       >  SRR19973063.606304/1‑150 (MQ=60)
                            TTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGC                                                                                                                       >  SRR19973063.2557457/1‑150 (MQ=60)
                             TTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCA                                                                                                                      >  SRR19973063.3869604/1‑150 (MQ=60)
                             GTTTGTCCATCCGTCGTACACCGACCCCCGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCA                                                                                                                      <  SRR19973063.3428801/150‑1 (MQ=60)
                              TTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAG                                                                                                                     <  SRR19973063.2210416/150‑1 (MQ=60)
                               TTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGA                                                                                                                    <  SRR19973063.4222269/150‑1 (MQ=60)
                               TTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGA                                                                                                                    >  SRR19973063.4930425/1‑150 (MQ=60)
                                TGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGAT                                                                                                                   >  SRR19973063.2735522/1‑150 (MQ=60)
                                 GTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATC                                                                                                                  >  SRR19973063.2096573/1‑150 (MQ=60)
                                  TCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCA                                                                                                                 <  SRR19973063.4883754/150‑1 (MQ=60)
                                  TCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCA                                                                                                                 >  SRR19973063.774423/1‑150 (MQ=60)
                                  TCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCA                                                                                                                 <  SRR19973063.4618030/150‑1 (MQ=60)
                                     ATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAG                                                                                                              >  SRR19973063.3820291/1‑150 (MQ=60)
                                     ATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATACCGCGACAGTTGTCAGAAGCAGGCAGATCACAG                                                                                                              >  SRR19973063.3820495/1‑150 (MQ=60)
                                      TCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGT                                                                                                             >  SRR19973063.177957/1‑150 (MQ=60)
                                       CAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTT                                                                                                            <  SRR19973063.654406/150‑1 (MQ=60)
                                        AGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTG                                                                                                           <  SRR19973063.401152/150‑1 (MQ=60)
                                         GTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGA                                                                                                          <  SRR19973063.5070010/150‑1 (MQ=60)
                                         GTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGA                                                                                                          <  SRR19973063.14029/150‑1 (MQ=60)
                                         GTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGA                                                                                                          <  SRR19973063.14031/150‑1 (MQ=60)
                                         GTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGA                                                                                                          >  SRR19973063.2770688/1‑150 (MQ=60)
                                         GTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGA                                                                                                          >  SRR19973063.2482994/1‑150 (MQ=60)
                                            GTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCA                                                                                                       <  SRR19973063.1316354/150‑1 (MQ=60)
                                            GTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCA                                                                                                       <  SRR19973063.1316308/150‑1 (MQ=60)
                                             TACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAA                                                                                                      <  SRR19973063.1576797/150‑1 (MQ=60)
                                              ACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAG                                                                                                     >  SRR19973063.2025520/1‑150 (MQ=60)
                                               CACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGC                                                                                                    >  SRR19973063.3656575/1‑150 (MQ=60)
                                                ACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCG                                                                                                   <  SRR19973063.1092752/150‑1 (MQ=60)
                                                ACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCG                                                                                                   >  SRR19973063.3872916/1‑150 (MQ=60)
                                                ACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCG                                                                                                   >  SRR19973063.294529/1‑150 (MQ=60)
                                                 CCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGA                                                                                                  <  SRR19973063.4620992/150‑1 (MQ=60)
                                                  CGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGGTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAA                                                                                                 >  SRR19973063.2392699/1‑150 (MQ=60)
                                                   GACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAAT                                                                                                >  SRR19973063.552368/1‑150 (MQ=60)
                                                   GACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAAT                                                                                                <  SRR19973063.2123574/150‑1 (MQ=60)
                                                    ACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATG                                                                                               >  SRR19973063.550166/1‑150 (MQ=60)
                                                      CCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTC                                                                                             >  SRR19973063.4819780/1‑150 (MQ=60)
                                                      CCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTC                                                                                             <  SRR19973063.2993940/150‑1 (MQ=60)
                                                      CCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTC                                                                                             >  SRR19973063.2203781/1‑150 (MQ=60)
                                                         AGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGAC                                                                                          <  SRR19973063.4004731/150‑1 (MQ=60)
                                                         AGTTGCTTCGCATGCACTTGTGTTGATCATCAAAGGAGTAGCTACAGTTGTCTTAGACGTGCTATCGAAGGTTACGCCAACTTTGGATACTTGCATTCCGATTCCGTTGTCAGTAGAAGTCAGATCACAGTTGATCTAGCGAATGTCGAC                                                                                          <  SRR19973063.5077030/150‑1 (MQ=60)
                                                              CTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCA                                                                                     >  SRR19973063.3403697/1‑150 (MQ=60)
                                                              CTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCA                                                                                     >  SRR19973063.3716484/1‑150 (MQ=60)
                                                                  GCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCTAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGACGCAGGCAGATCACAGTAGATCAAGCGAATGTCGACGGCCACTTT                                                                                 >  SRR19973063.2096217/1‑150 (MQ=60)
                                                                   CCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTA                                                                                <  SRR19973063.4377092/150‑1 (MQ=60)
                                                                     TGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATT                                                                              <  SRR19973063.1082936/150‑1 (MQ=60)
                                                                       CACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGC                                                                            >  SRR19973063.4501133/1‑150 (MQ=60)
                                                                       CACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGC                                                                            >  SRR19973063.2383261/1‑150 (MQ=60)
                                                                        ACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCT                                                                           <  SRR19973063.4699760/150‑1 (MQ=60)
                                                                        ACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATAAAGAGAATGTCGACGGCCACTTTATTGCT                                                                           <  SRR19973063.2047406/150‑1 (MQ=60)
                                                                             TGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGAT                                                                      >  SRR19973063.2602437/1‑150 (MQ=60)
                                                                                TGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCT                                                                   <  SRR19973063.1465881/150‑1 (MQ=60)
                                                                                     AACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCTG                                                              >  SRR19973063.2379953/1‑150 (MQ=60)
                                                                                      ACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGT                                                             <  SRR19973063.2256784/150‑1 (MQ=60)
                                                                                      ACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGT                                                             >  SRR19973063.1141204/1‑150 (MQ=60)
                                                                                       CAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTT                                                            <  SRR19973063.980187/150‑1 (MQ=60)
                                                                                          AGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTNT                                                         <  SRR19973063.3912736/150‑1 (MQ=60)
                                                                                           GGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATA                                                        <  SRR19973063.207049/150‑1 (MQ=60)
                                                                                             CGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATG                                                      >  SRR19973063.2085691/1‑150 (MQ=60)
                                                                                             CGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATG                                                      >  SRR19973063.690434/1‑150 (MQ=60)
                                                                                              GTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGG                                                     >  SRR19973063.436421/1‑150 (MQ=60)
                                                                                              GTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGG                                                     >  SRR19973063.4997157/1‑150 (MQ=60)
                                                                                               TAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGG                                                    <  SRR19973063.1910563/150‑1 (MQ=60)
                                                                                                   ACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGT                                                >  SRR19973063.2128860/1‑150 (MQ=60)
                                                                                                   ACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGT                                                >  SRR19973063.2128972/1‑150 (MQ=60)
                                                                                                     CAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCG                                              <  SRR19973063.1759417/150‑1 (MQ=60)
                                                                                                     CAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCG                                              >  SRR19973063.4467105/1‑150 (MQ=60)
                                                                                                      AGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGT                                             >  SRR19973063.4918893/1‑150 (MQ=60)
                                                                                                           TCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTT                                        <  SRR19973063.146845/150‑1 (MQ=60)
                                                                                                            CTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTG                                       <  SRR19973063.4664551/150‑1 (MQ=60)
                                                                                                             TTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGT                                      <  SRR19973063.2644310/150‑1 (MQ=60)
                                                                                                              TAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTC                                     >  SRR19973063.930757/1‑150 (MQ=60)
                                                                                                               AGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCC                                    <  SRR19973063.4590086/150‑1 (MQ=60)
                                                                                                                GCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCA                                   <  SRR19973063.3729714/150‑1 (MQ=60)
                                                                                                                 CCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAA                                  <  SRR19973063.1819675/150‑1 (MQ=60)
                                                                                                                   GTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGA                                <  SRR19973063.293559/150‑1 (MQ=60)
                                                                                                                    TGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTCTTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGAT                               >  SRR19973063.4842447/1‑150 (MQ=60)
                                                                                                                    TGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGAT                               >  SRR19973063.898209/1‑150 (MQ=60)
                                                                                                                       TATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTA                            <  SRR19973063.3848435/150‑1 (MQ=60)
                                                                                                                       TATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTA                            >  SRR19973063.4176884/1‑150 (MQ=60)
                                                                                                                       TATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTA                            >  SRR19973063.4176692/1‑150 (MQ=60)
                                                                                                                         TCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATG                          <  SRR19973063.4092533/150‑1 (MQ=60)
                                                                                                                         TCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATG                          <  SRR19973063.4092433/150‑1 (MQ=60)
                                                                                                                           GAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTT                        >  SRR19973063.4440466/1‑150 (MQ=60)
                                                                                                                            AAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTT                       <  SRR19973063.130663/150‑1 (MQ=60)
                                                                                                                            AAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTT                       <  SRR19973063.130667/150‑1 (MQ=60)
                                                                                                                            AAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTT                       >  SRR19973063.3407930/1‑150 (MQ=60)
                                                                                                                            AAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTT                       >  SRR19973063.3588476/1‑150 (MQ=60)
                                                                                                                             AGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTT                      <  SRR19973063.367533/150‑1 (MQ=60)
                                                                                                                                TTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTAT                   >  SRR19973063.3648029/1‑150 (MQ=60)
                                                                                                                                 TACGCCAAGTTTGGATACAGGCTGTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATC                  <  SRR19973063.4669100/150‑1 (MQ=60)
                                                                                                                                     CCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATA              <  SRR19973063.4264950/150‑1 (MQ=60)
                                                                                                                                        ACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCT           <  SRR19973063.1255395/150‑1 (MQ=60)
                                                                                                                                         CTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTT          >  SRR19973063.1224113/1‑150 (MQ=60)
                                                                                                                                          TTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTC         <  SRR19973063.2021249/150‑1 (MQ=60)
                                                                                                                                          TTTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTC         <  SRR19973063.2021212/150‑1 (MQ=60)
                                                                                                                                           TTGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCT        <  SRR19973063.4484772/150‑1 (MQ=60)
                                                                                                                                            TGGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTG       <  SRR19973063.4795675/150‑1 (MQ=60)
                                                                                                                                             GGATACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGG      <  SRR19973063.1513723/150‑1 (MQ=60)
                                                                                                                                                 ACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGA  >  SRR19973063.4156601/1‑150 (MQ=60)
                                                                                                                                                 ACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGA  >  SRR19973063.4157203/1‑150 (MQ=60)
                                                                                                                                                 ACAGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGA  <  SRR19973063.4919458/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
GGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGA  >  CP009273/747826‑748120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑