Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 868,500 A→T 100% S22S (TCA→TCT)  yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273868,5000AT95.8% 750.2 / 15.4 237S22S (TCA→TCT) yliEputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (6/4);  new base T (124/103);  total (130/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 242 total aligned reads displayed.

ATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGA  >  CP009273/868355‑868643
                                                                                                                                                 |                                                                                                                                               
aTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCttt                                                                                                                                               >  2:3054650/1‑148 (MQ=255)
 tttGGTTAAAAAGCCGCAGCAAGGGTCAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                              <  1:1177677/148‑1 (MQ=255)
 tttGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                              >  2:2105700/1‑148 (MQ=255)
  ttGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTa                                                                                                                                             <  1:1032597/148‑1 (MQ=255)
  ttGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTa                                                                                                                                             <  1:195467/148‑1 (MQ=255)
   tGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTAt                                                                                                                                            <  2:3098989/148‑1 (MQ=255)
    ggTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTAtt                                                                                                                                           <  1:258796/148‑1 (MQ=255)
     gTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTg                                                                                                                                          <  1:755145/148‑1 (MQ=255)
           aaGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCttt                                                                                                                                    <  1:1883477/148‑1 (MQ=255)
              ccGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCtttttt                                                                                                                                 >  2:1951964/1‑148 (MQ=255)
                gcagcaAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTtc                                                                                                                               <  1:989285/148‑1 (MQ=255)
                    caAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTtcatca                                                                                                                           <  2:2287667/148‑1 (MQ=255)
                    caAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTtcatca                                                                                                                           >  2:1519984/1‑148 (MQ=255)
                       gggACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTa                                                                                                                        >  1:3348160/1‑148 (MQ=255)
                         gACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAAc                                                                                                                      <  2:1626213/148‑1 (MQ=255)
                         gACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAAc                                                                                                                      <  1:92651/148‑1 (MQ=255)
                           cAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCTCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTa                                                                                                                    >  1:3284148/1‑148 (MQ=255)
                           cAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTa                                                                                                                    <  1:409685/148‑1 (MQ=255)
                              tttttGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCa                                                                                                                 >  1:1020895/1‑148 (MQ=255)
                              tttttGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCa                                                                                                                 >  1:2796149/1‑148 (MQ=255)
                               ttttGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCaa                                                                                                                >  1:1759414/1‑148 (MQ=255)
                                    cAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGt                                                                                                           >  2:1532355/1‑148 (MQ=255)
                                         gCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAAATATCAACTGGTATAgg                                                                                                      >  1:3386597/1‑148 (MQ=255)
                                           acaGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAg                                                                                                    <  2:1756339/148‑1 (MQ=255)
                                                 ttCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGcgcgcg                                                                                              >  2:1487520/1‑148 (MQ=255)
                                                  tCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCgg                                                                                             >  2:2546266/1‑148 (MQ=255)
                                                     gATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATAAACTGTTATAGGATCGCGAGGTaa                                                                                          >  1:3098062/1‑148 (MQ=255)
                                                      aTAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTaaa                                                                                         <  1:2739173/148‑1 (MQ=255)
                                                         gTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACg                                                                                      <  2:1292760/148‑1 (MQ=255)
                                                          ttATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGt                                                                                     <  2:1685814/148‑1 (MQ=255)
                                                           tATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTg                                                                                    <  2:2641338/148‑1 (MQ=255)
                                                           tATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTg                                                                                    >  2:632039/1‑148 (MQ=255)
                                                           tATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTg                                                                                    >  2:3511633/1‑148 (MQ=255)
                                                           tATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTg                                                                                    >  1:146719/1‑148 (MQ=255)
                                                            aTTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGc                                                                                   >  2:1263779/1‑148 (MQ=255)
                                                              tttGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCg                                                                                 <  1:2416836/148‑1 (MQ=255)
                                                               ttGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGa                                                                                >  2:2358634/1‑148 (MQ=255)
                                                               ttGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGa                                                                                >  2:2039860/1‑148 (MQ=255)
                                                                   tAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCAGATAGc                                                                            <  1:1310409/148‑1 (MQ=255)
                                                                       tGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCt                                                                        <  1:3298242/148‑1 (MQ=255)
                                                                        gTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCtt                                                                       >  1:2660573/1‑148 (MQ=255)
                                                                        gTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCtt                                                                       >  1:446684/1‑148 (MQ=255)
                                                                         tATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCttt                                                                      >  1:653883/1‑148 (MQ=255)
                                                                          aTTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTg                                                                     >  2:2665830/1‑148 (MQ=255)
                                                                            tAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGaa                                                                   >  2:3251736/1‑148 (MQ=255)
                                                                             aaCATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAAc                                                                  <  1:1747270/148‑1 (MQ=255)
                                                                              aCATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACt                                                                 >  1:2200805/1‑148 (MQ=255)
                                                                              aCATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACt                                                                 >  1:868878/1‑148 (MQ=255)
                                                                              aCATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACt                                                                 >  1:785199/1‑148 (MQ=255)
                                                                               cATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACtt                                                                >  1:3275974/1‑148 (MQ=255)
                                                                               cATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATAATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACtt                                                                >  1:714251/1‑148 (MQ=255)
                                                                                aTGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTa                                                               >  2:1009725/1‑148 (MQ=255)
                                                                                 tGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTAt                                                              >  1:3557027/1‑148 (MQ=255)
                                                                                 tGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTAt                                                              <  2:2914330/148‑1 (MQ=255)
                                                                                  gCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTAtt                                                             >  1:2515559/1‑148 (MQ=255)
                                                                                   cTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTc                                                            >  2:2212171/1‑148 (MQ=255)
                                                                                     gAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAg                                                          >  2:2023352/1‑148 (MQ=255)
                                                                                      aGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGa                                                         >  2:3030294/1‑148 (MQ=255)
                                                                                      aGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGa                                                         <  2:3081279/148‑1 (MQ=255)
                                                                                        tttATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGaaa                                                       >  2:922368/1‑148 (MQ=255)
                                                                                         ttATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGaaaa                                                      <  1:2105700/148‑1 (MQ=255)
                                                                                          tataCGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGaaaaa                                                     <  2:2536139/148‑1 (MQ=255)
                                                                                             aCGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGt                                                  >  1:2925539/1‑148 (MQ=255)
                                                                                              cGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGtt                                                 <  2:1684277/148‑1 (MQ=255)
                                                                                              cGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGtt                                                 <  2:2552286/148‑1 (MQ=255)
                                                                                               gAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTg                                                <  1:2961487/148‑1 (MQ=255)
                                                                                               gAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTg                                                <  2:3164552/148‑1 (MQ=255)
                                                                                                 aaagataaagatAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGc                                              >  1:2105400/1‑148 (MQ=255)
                                                                                                 aaagataaagatAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGc                                              <  1:2290085/148‑1 (MQ=255)
                                                                                                 aaagataaagatAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGc                                              <  2:2813272/148‑1 (MQ=255)
                                                                                                 aaagataaagatAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGc                                              <  2:3185343/148‑1 (MQ=255)
                                                                                                   agataaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTa                                            <  1:1583715/148‑1 (MQ=255)
                                                                                                    gataaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTAt                                           >  2:2835889/1‑148 (MQ=255)
                                                                                                      taaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTAttt                                         <  1:80714/148‑1 (MQ=255)
                                                                                                      taaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTAttt                                         <  1:1845095/148‑1 (MQ=255)
                                                                                                         agataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCtt                                      >  2:2784027/1‑148 (MQ=255)
                                                                                                          gataaGGCTGATAATTTTATTTTTATTGGCAGCACAGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTAAGAAAAACGTTGGCTATTTCttt                                     >  1:878791/1‑148 (MQ=255)
                                                                                                             aaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTaaa                                  >  1:966708/1‑148 (MQ=255)
                                                                                                                gCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGat                               >  2:488112/1‑148 (MQ=255)
                                                                                                                gCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGat                               >  2:1357826/1‑148 (MQ=255)
                                                                                                                 cTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGata                              <  1:3471166/148‑1 (MQ=255)
                                                                                                                  tGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTTGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGatat                             <  2:1586302/148‑1 (MQ=255)
                                                                                                                  tGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGatat                             >  1:2340425/1‑148 (MQ=255)
                                                                                                                        ttttatttttatTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACg                       >  1:1384484/1‑148 (MQ=255)
                                                                                                                        ttttatttttatTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACg                       <  1:1254058/148‑1 (MQ=255)
                                                                                                                         tttatttttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGt                      >  1:1556447/1‑148 (MQ=255)
                                                                                                                          ttatttttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAAGGtt                     >  1:1627885/1‑148 (MQ=255)
                                                                                                                          ttatttttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGtt                     >  2:854803/1‑148 (MQ=255)
                                                                                                                            atttttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCg                   >  2:3547343/1‑148 (MQ=255)
                                                                                                                            atttttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCg                   >  2:72606/1‑148 (MQ=255)
                                                                                                                             tttttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCgg                  >  1:2441112/1‑148 (MQ=255)
                                                                                                                              ttttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTACAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGc                 <  2:3369355/148‑1 (MQ=255)
                                                                                                                               tttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGcc                <  1:3353952/148‑1 (MQ=255)
                                                                                                                                     ggCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACa          <  1:549978/148‑1 (MQ=255)
                                                                                                                                        agcaCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTa       <  2:876457/148‑1 (MQ=255)
                                                                                                                                        agcaCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTa       <  2:1648848/148‑1 (MQ=255)
                                                                                                                                         gcaCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTaa      <  1:3054650/148‑1 (MQ=255)
                                                                                                                                             tGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGa  <  2:2378638/148‑1 (MQ=255)
                                                                                                                                             tGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGa  >  2:2184510/1‑148 (MQ=255)
                                                                                                                                             tGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGa  >  2:543081/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGA  >  CP009273/868355‑868643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCA  >  CP009273/868354‑868647
                                                                                                                                                  |                                                                                                                                                   
AATTTGGTTAAAAAGCCGCATCAAGGGACAATTTTTGCAGCGGCACAGAGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTT                                                                                                                                                  <  SRR19973067.3439233/150‑1 (MQ=60)
AATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTT                                                                                                                                                  >  SRR19973067.961367/1‑150 (MQ=60)
  TTTGGTTAAAAAGCCGCAGCAAGGGTCAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTAT                                                                                                                                                <  SRR19973067.1185462/150‑1 (MQ=60)
   TTGGTTAACAAGCCGCAGCAAGGGACAATTTTTTCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGATTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATT                                                                                                                                               <  SRR19973067.1039710/150‑1 (MQ=60)
   TTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATT                                                                                                                                               <  SRR19973067.1038223/150‑1 (MQ=60)
   TTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATT                                                                                                                                               <  SRR19973067.196974/150‑1 (MQ=60)
     GGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGG                                                                                                                                             <  SRR19973067.260566/150‑1 (MQ=60)
      GTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGT                                                                                                                                            <  SRR19973067.760392/150‑1 (MQ=60)
          AAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTT                                                                                                                                        >  SRR19973067.460904/1‑150 (MQ=60)
            AAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTT                                                                                                                                      <  SRR19973067.1896336/150‑1 (MQ=60)
             AGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTT                                                                                                                                     <  SRR19973067.2953320/150‑1 (MQ=60)
              GCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTT                                                                                                                                    <  SRR19973067.1306875/150‑1 (MQ=60)
                 GCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCAT                                                                                                                                 <  SRR19973067.996094/150‑1 (MQ=60)
                 GCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCAT                                                                                                                                 <  SRR19973067.996089/150‑1 (MQ=60)
                  CAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATC                                                                                                                                <  SRR19973067.2189085/150‑1 (MQ=60)
                  CAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATC                                                                                                                                >  SRR19973067.800172/1‑150 (MQ=60)
                  CAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATC                                                                                                                                <  SRR19973067.266107/150‑1 (MQ=60)
                   AGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCA                                                                                                                               >  SRR19973067.882309/1‑150 (MQ=60)
                      AAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTA                                                                                                                            >  SRR19973067.3369384/1‑150 (MQ=60)
                       AGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAA                                                                                                                           <  SRR19973067.857297/150‑1 (MQ=60)
                       AGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAA                                                                                                                           >  SRR19973067.222338/1‑150 (MQ=60)
                          GACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCTCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTA                                                                                                                        >  SRR19973067.3304673/1‑150 (MQ=60)
                          GACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCTCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTA                                                                                                                        >  SRR19973067.3304753/1‑150 (MQ=60)
                          GACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTA                                                                                                                        <  SRR19973067.93627/150‑1 (MQ=60)
                            CAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATC                                                                                                                      <  SRR19973067.412540/150‑1 (MQ=60)
                             AATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCA                                                                                                                     >  SRR19973067.1027906/1‑150 (MQ=60)
                             AATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCA                                                                                                                     >  SRR19973067.2814182/1‑150 (MQ=60)
                              ATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAA                                                                                                                    >  SRR19973067.1771392/1‑150 (MQ=60)
                              ATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAA                                                                                                                    >  SRR19973067.1895556/1‑150 (MQ=60)
                                TTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACT                                                                                                                  <  SRR19973067.1962384/150‑1 (MQ=60)
                                     CAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTAT                                                                                                             >  SRR19973067.2439236/1‑150 (MQ=60)
                                       GCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCG                                                                                                           <  SRR19973067.3550543/150‑1 (MQ=60)
                                        CGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAAATATCAACTGGTATAGG                                                                                                          >  SRR19973067.3408119/1‑150 (MQ=60)
                                         GGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGA                                                                                                         >  SRR19973067.2518607/1‑150 (MQ=60)
                                            ACAGCGTTCAGATAGTTATATTGTTAAATGAATTAACATGCTGAGTTTATACGAAAAGATAAATATAAGGCTGATAATTTTATGTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCATCTGGTATCGGAGCG                                                                                                      >  SRR19973067.425144/1‑150 (MQ=60)
                                                    CAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATAAACTGTTATAGGATCGCGAGGTAA                                                                                              >  SRR19973067.3117771/1‑150 (MQ=60)
                                                      GATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAA                                                                                            >  SRR19973067.572939/1‑150 (MQ=60)
                                                      GATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGATGATAATTTTATTTTTATTGGCAGCAATGTCTTTTATTGGTCTTTTTTTCATAATTAACTATCAACTGGTATCGGAGAGCGCGGTAAAA                                                                                            >  SRR19973067.265168/1‑150 (MQ=60)
                                                       ATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAAC                                                                                           <  SRR19973067.2756867/150‑1 (MQ=60)
                                                         AGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGT                                                                                         >  SRR19973067.4133/1‑150 (MQ=60)
                                                         AGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTANTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATGGGAGCGCGCGGTAAAACGT                                                                                         >  SRR19973067.1818219/1‑150 (MQ=60)
                                                          GTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTG                                                                                        <  SRR19973067.3308028/150‑1 (MQ=60)
                                                          GTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTG                                                                                        >  SRR19973067.147990/1‑150 (MQ=60)
                                                            TATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCC                                                                                      >  SRR19973067.2492769/1‑150 (MQ=60)
                                                             ATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCG                                                                                     <  SRR19973067.3319035/150‑1 (MQ=60)
                                                             ATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCG                                                                                     >  SRR19973067.1207824/1‑150 (MQ=60)
                                                               TTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGAT                                                                                   <  SRR19973067.2166142/150‑1 (MQ=60)
                                                               TTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGAT                                                                                   <  SRR19973067.2432668/150‑1 (MQ=60)
                                                                 TGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAG                                                                                 >  SRR19973067.2118317/1‑150 (MQ=60)
                                                                   TTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCC                                                                               <  SRR19973067.3373977/150‑1 (MQ=60)
                                                                    TAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCAGATAGCCG                                                                              <  SRR19973067.1319356/150‑1 (MQ=60)
                                                                    TAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCAGATAGCCG                                                                              <  SRR19973067.1319397/150‑1 (MQ=60)
                                                                       ATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTT                                                                           >  SRR19973067.449786/1‑150 (MQ=60)
                                                                       ATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTT                                                                           >  SRR19973067.3451493/1‑150 (MQ=60)
                                                                       ATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTT                                                                           >  SRR19973067.2677690/1‑150 (MQ=60)
                                                                        TGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTT                                                                          >  SRR19973067.658327/1‑150 (MQ=60)
                                                                        TGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTT                                                                          >  SRR19973067.659073/1‑150 (MQ=60)
                                                                        TGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTT                                                                          <  SRR19973067.744161/150‑1 (MQ=60)
                                                                        TGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTT                                                                          <  SRR19973067.3319012/150‑1 (MQ=60)
                                                                         GTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTG                                                                         <  SRR19973067.876644/150‑1 (MQ=60)
                                                                             TAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACT                                                                     >  SRR19973067.790629/1‑150 (MQ=60)
                                                                             TAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACT                                                                     >  SRR19973067.874688/1‑150 (MQ=60)
                                                                             TAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACT                                                                     >  SRR19973067.2215487/1‑150 (MQ=60)
                                                                             TAACAGGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTGTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACT                                                                     >  SRR19973067.253754/1‑150 (MQ=60)
                                                                              AACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTT                                                                    <  SRR19973067.1759191/150‑1 (MQ=60)
                                                                              AACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTT                                                                    >  SRR19973067.3296481/1‑150 (MQ=60)
                                                                              AACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATAATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTGTGAACTT                                                                    >  SRR19973067.719196/1‑150 (MQ=60)
                                                                                CATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTAT                                                                  >  SRR19973067.3579931/1‑150 (MQ=60)
                                                                                CATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTAT                                                                  >  SRR19973067.3579439/1‑150 (MQ=60)
                                                                                CATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTAT                                                                  >  SRR19973067.3365171/1‑150 (MQ=60)
                                                                                 ATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCGTTGAACTTATT                                                                 >  SRR19973067.2532053/1‑150 (MQ=60)
                                                                                  TGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTC                                                                <  SRR19973067.1760740/150‑1 (MQ=60)
                                                                                   GCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCA                                                               >  SRR19973067.1067996/1‑150 (MQ=60)
                                                                                    CTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAG                                                              >  SRR19973067.484548/1‑150 (MQ=60)
                                                                                       AGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAA                                                           >  SRR19973067.1744724/1‑150 (MQ=60)
                                                                                       AGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAA                                                           <  SRR19973067.2852773/150‑1 (MQ=60)
                                                                                        GTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAA                                                          <  SRR19973067.1376492/150‑1 (MQ=60)
                                                                                          TTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAAC                                                        <  SRR19973067.2119599/150‑1 (MQ=60)
                                                                                          TTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAAC                                                        <  SRR19973067.2119678/150‑1 (MQ=60)
                                                                                            ATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGT                                                      >  SRR19973067.930372/1‑150 (MQ=60)
                                                                                            ATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGT                                                      >  SRR19973067.2944131/1‑150 (MQ=60)
                                                                                                GAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGC                                                  >  SRR19973067.158966/1‑150 (MQ=60)
                                                                                                GAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGC                                                  >  SRR19973067.2848687/1‑150 (MQ=60)
                                                                                                GAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGC                                                  >  SRR19973067.2119375/1‑150 (MQ=60)
                                                                                                GAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGC                                                  <  SRR19973067.2980194/150‑1 (MQ=60)
                                                                                                 AAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCT                                                 >  SRR19973067.3223964/1‑150 (MQ=60)
                                                                                                  AAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTA                                                <  SRR19973067.2305217/150‑1 (MQ=60)
                                                                                                    AGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATT                                              <  SRR19973067.3112874/150‑1 (MQ=60)
                                                                                                    AGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATT                                              <  SRR19973067.1594713/150‑1 (MQ=60)
                                                                                                    AGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATT                                              <  SRR19973067.1594558/150‑1 (MQ=60)
                                                                                                       TAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCT                                           <  SRR19973067.1857675/150‑1 (MQ=60)
                                                                                                       TAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCT                                           <  SRR19973067.81609/150‑1 (MQ=60)
                                                                                                       TAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCT                                           <  SRR19973067.81596/150‑1 (MQ=60)
                                                                                                         AAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACAGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTAAGAAAAACGTTGGCTATTTCTTT                                         >  SRR19973067.884648/1‑150 (MQ=60)
                                                                                                            ATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAA                                      <  SRR19973067.982204/150‑1 (MQ=60)
                                                                                                            ATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAA                                      <  SRR19973067.766497/150‑1 (MQ=60)
                                                                                                            ATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAA                                      >  SRR19973067.973330/1‑150 (MQ=60)
                                                                                                             TAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAG                                     <  SRR19973067.953184/150‑1 (MQ=60)
                                                                                                               AGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAAATTATTAAGAAAAACGTTGGCTATTTCTTTAAAGAT                                   >  SRR19973067.1217921/1‑150 (MQ=60)
                                                                                                                GGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATA                                  <  SRR19973067.3067364/150‑1 (MQ=60)
                                                                                                                 GCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATAT                                 >  SRR19973067.2355736/1‑150 (MQ=60)
                                                                                                                 GCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATAT                                 >  SRR19973067.2394583/1‑150 (MQ=60)
                                                                                                                  CTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATT                                <  SRR19973067.3493214/150‑1 (MQ=60)
                                                                                                                   TGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTG                               >  SRR19973067.2405409/1‑150 (MQ=60)
                                                                                                                      TAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAAC                            <  SRR19973067.3388752/150‑1 (MQ=60)
                                                                                                                      TAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAAC                            <  SRR19973067.3388029/150‑1 (MQ=60)
                                                                                                                       AATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACG                           >  SRR19973067.1393907/1‑150 (MQ=60)
                                                                                                                        ATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGT                          >  SRR19973067.1567141/1‑150 (MQ=60)
                                                                                                                        ATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGT                          >  SRR19973067.1567053/1‑150 (MQ=60)
                                                                                                                        ATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCGTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGT                          >  SRR19973067.1406639/1‑150 (MQ=60)
                                                                                                                         TTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAAGGTT                         >  SRR19973067.1639060/1‑150 (MQ=60)
                                                                                                                         TTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTT                         <  SRR19973067.1262541/150‑1 (MQ=60)
                                                                                                                           TTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCG                       <  SRR19973067.2010377/150‑1 (MQ=60)
                                                                                                                            TATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGG                      >  SRR19973067.661050/1‑150 (MQ=60)
                                                                                                                            TATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGG                      >  SRR19973067.231844/1‑150 (MQ=60)
                                                                                                                            TATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGG                      >  SRR19973067.2457140/1‑150 (MQ=60)
                                                                                                                               TTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCC                   <  SRR19973067.2750737/150‑1 (MQ=60)
                                                                                                                                TTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCT                  <  SRR19973067.3375233/150‑1 (MQ=60)
                                                                                                                                      GGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATT            <  SRR19973067.553605/150‑1 (MQ=60)
                                                                                                                                      GGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTATTTAAAGATATTGAACGTTCGGCCCTGACATT            >  SRR19973067.1837941/1‑150 (MQ=60)
                                                                                                                                        CAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAA          <  SRR19973067.1530149/150‑1 (MQ=60)
                                                                                                                                         AGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAA         >  SRR19973067.299963/1‑150 (MQ=60)
                                                                                                                                         AGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAA         >  SRR19973067.1662600/1‑150 (MQ=60)
                                                                                                                                         AGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAA         >  SRR19973067.2772560/1‑150 (MQ=60)
                                                                                                                                          GCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAG        <  SRR19973067.3074111/150‑1 (MQ=60)
                                                                                                                                            ACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGA      >  SRR19973067.3223628/1‑150 (MQ=60)
                                                                                                                                               GTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTC   >  SRR19973067.2160415/1‑150 (MQ=60)
                                                                                                                                                TCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCA  >  SRR19973067.1772616/1‑150 (MQ=60)
                                                                                                                                                TCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCA  <  SRR19973067.1177959/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                   
AATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCA  >  CP009273/868354‑868647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑