Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 868,500 A→T 100% S22S (TCA→TCT)  yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273868,5000AT99.2% 596.2 / ‑4.4 178S22S (TCA→TCT) yliEputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base T (86/90);  total (86/92)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

AATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTC  >  CP009273/868354‑868646
                                                                                                                                                  |                                                                                                                                                  
aaTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                                 >  1:4465656/1‑150 (MQ=255)
aaTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                                 <  2:1225266/150‑1 (MQ=255)
aaTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                                 <  2:127559/150‑1 (MQ=255)
aaTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                                 <  2:143063/150‑1 (MQ=255)
  tttGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTAt                                                                                                                                               <  2:2720251/150‑1 (MQ=255)
   ttGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTAtt                                                                                                                                              <  1:2246679/150‑1 (MQ=255)
   ttGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTAtt                                                                                                                                              <  2:1801958/150‑1 (MQ=255)
    tGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTg                                                                                                                                             >  1:2713841/1‑150 (MQ=255)
     ggTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTgg                                                                                                                                            >  1:2888204/1‑150 (MQ=255)
       ttAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTc                                                                                                                                          <  2:4791102/150‑1 (MQ=255)
        tAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCt                                                                                                                                         <  1:828522/150‑1 (MQ=255)
          aaaaGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCttt                                                                                                                                       >  1:3363761/1‑150 (MQ=255)
             aGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCtttttt                                                                                                                                    <  1:1125916/150‑1 (MQ=255)
             aGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCtttttt                                                                                                                                    <  2:3018420/150‑1 (MQ=255)
             aGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCtttttt                                                                                                                                    <  1:2591170/150‑1 (MQ=255)
              gCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCttttttt                                                                                                                                   <  1:4010935/150‑1 (MQ=255)
                 gcagcaAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTtcat                                                                                                                                <  1:3986822/150‑1 (MQ=255)
                   agcaAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTtcatca                                                                                                                              <  2:1469759/150‑1 (MQ=255)
                     caAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCAtt                                                                                                                            <  2:2264347/150‑1 (MQ=255)
                     caAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCAtt                                                                                                                            <  2:587390/150‑1 (MQ=255)
                      aaGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTACATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTa                                                                                                                           <  1:4528042/150‑1 (MQ=255)
                        gggACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAAc                                                                                                                         <  1:3642298/150‑1 (MQ=255)
                             aaTTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCa                                                                                                                    <  1:2963737/150‑1 (MQ=255)
                              aTTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCaa                                                                                                                   >  1:1198541/1‑150 (MQ=255)
                                 tttGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTg                                                                                                                <  2:642163/150‑1 (MQ=255)
                                      aGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATc                                                                                                           >  1:294105/1‑150 (MQ=255)
                                      aGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATc                                                                                                           <  1:3717687/150‑1 (MQ=255)
                                         ggCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGa                                                                                                        >  1:508940/1‑150 (MQ=255)
                                              aGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAgcgcg                                                                                                   >  2:2767755/1‑150 (MQ=255)
                                                 gTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCgg                                                                                                <  1:108215/150‑1 (MQ=255)
                                                   tCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTa                                                                                              >  1:944457/1‑150 (MQ=255)
                                                       aTAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAAc                                                                                          <  2:3306461/150‑1 (MQ=255)
                                                       aTAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAAc                                                                                          <  1:1703673/150‑1 (MQ=255)
                                                       aTAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAAc                                                                                          <  1:4132647/150‑1 (MQ=255)
                                                       aTAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAAc                                                                                          <  2:3195635/150‑1 (MQ=255)
                                                        tAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACg                                                                                         >  2:763183/1‑150 (MQ=255)
                                                        tAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACg                                                                                         >  2:2360390/1‑150 (MQ=255)
                                                          gTTATTTTTTTAAATGTATTAACATGCTGATTTTATACGAAAATATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTg                                                                                       <  2:533948/150‑1 (MQ=255)
                                                              ttttGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGa                                                                                   <  2:2505386/150‑1 (MQ=255)
                                                               tttGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGAt                                                                                  <  2:3615819/150‑1 (MQ=255)
                                                                ttGTTCACTGTATGAACATTCTGAGTTTATACGAAACGATACAGATAAGGCTGATAATTTTATTTTTAGTGGCAGCACTGTCTTTTATTGGTCATTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATa                                                                                 <  2:4752834/150‑1 (MQ=255)
                                                                   ttAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGcc                                                                              <  1:5056688/150‑1 (MQ=255)
                                                                        tGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCttt                                                                         >  2:624078/1‑150 (MQ=255)
                                                                          tATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGa                                                                       >  2:3336054/1‑150 (MQ=255)
                                                                          tATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGa                                                                       <  2:1846920/150‑1 (MQ=255)
                                                                          tATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGa                                                                       <  2:4289606/150‑1 (MQ=255)
                                                                          tATTAACATGCTGAGTTTAAACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGa                                                                       <  2:2937089/150‑1 (MQ=255)
                                                                               aCATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCAATGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGATCGCGCGGTAAAACGTGACGATAGCCGCTTTGAACTTa                                                                  >  2:3475330/1‑150 (MQ=255)
                                                                                cATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTAt                                                                 <  2:3986138/150‑1 (MQ=255)
                                                                                cATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTAt                                                                 <  2:4324766/150‑1 (MQ=255)
                                                                                  tGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTc                                                               <  1:4313755/150‑1 (MQ=255)
                                                                                  tGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTc                                                               <  1:1245103/150‑1 (MQ=255)
                                                                                  tGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTc                                                               <  1:3824962/150‑1 (MQ=255)
                                                                                     tNAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGa                                                            >  1:1865742/1‑150 (MQ=255)
                                                                                      gAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGaa                                                           >  1:1282323/1‑150 (MQ=255)
                                                                                      gAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGaa                                                           >  2:559679/1‑150 (MQ=255)
                                                                                      gAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGaa                                                           >  2:3070446/1‑150 (MQ=255)
                                                                                       aGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGaaa                                                          >  2:4451793/1‑150 (MQ=255)
                                                                                       aGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGaaa                                                          >  1:2916397/1‑150 (MQ=255)
                                                                                          ttATACGAATAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAAc                                                       <  2:4735403/150‑1 (MQ=255)
                                                                                          ttATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAAc                                                       <  2:168972/150‑1 (MQ=255)
                                                                                           tataCGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACg                                                      >  2:2228432/1‑150 (MQ=255)
                                                                                           tataCGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACg                                                      <  1:3944059/150‑1 (MQ=255)
                                                                                             taCGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGtt                                                    <  1:723658/150‑1 (MQ=255)
                                                                                              aCGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTg                                                   <  1:2875387/150‑1 (MQ=255)
                                                                                               cGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTgg                                                  <  2:4919791/150‑1 (MQ=255)
                                                                                                gAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGc                                                 >  1:1088336/1‑150 (MQ=255)
                                                                                                gAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGc                                                 >  1:3753581/1‑150 (MQ=255)
                                                                                                 aaaaGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCt                                                <  1:207583/150‑1 (MQ=255)
                                                                                                 aaaaGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCt                                                <  2:4338741/150‑1 (MQ=255)
                                                                                                   aagataaagataAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTAt                                              <  2:4918584/150‑1 (MQ=255)
                                                                                                    agataaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTAtt                                             <  2:3224171/150‑1 (MQ=255)
                                                                                                       taaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCt                                          <  1:4707934/150‑1 (MQ=255)
                                                                                                       taaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCt                                          <  1:2236861/150‑1 (MQ=255)
                                                                                                          agataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGTGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTa                                       <  2:1681909/150‑1 (MQ=255)
                                                                                                          agataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTa                                       >  1:4341105/1‑150 (MQ=255)
                                                                                                            ataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACTTTGGCTATTTCTTTaaa                                     >  2:1510590/1‑150 (MQ=255)
                                                                                                               aGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGat                                  >  1:2926479/1‑150 (MQ=255)
                                                                                                                ggCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGata                                 >  1:3713056/1‑150 (MQ=255)
                                                                                                                 gCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCGACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGatat                                >  1:1234156/1‑150 (MQ=255)
                                                                                                                  cTGATAATTTTATTTTTATTGGCAGCAATGTCTTTTATTGGTCTTTTTTTCATCATTAAATATCAACTGGTATCGTAGCGCGCGGTAAAACTTGCCGATAGCCGCTTTGAACTTATTCAGAAAAAAGTTGGCTATTTATTTAAAGATAtt                               >  2:4640658/1‑150 (MQ=255)
                                                                                                                      tAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAAc                           <  2:3626280/150‑1 (MQ=255)
                                                                                                                           ttatttttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCg                      >  1:2305338/1‑150 (MQ=255)
                                                                                                                            tatttttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCgg                     <  2:1715182/150‑1 (MQ=255)
                                                                                                                                 ttattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCACTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTg                >  2:1706629/1‑150 (MQ=255)
                                                                                                                                  tattGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGa               <  2:2408253/150‑1 (MQ=255)
                                                                                                                                   attGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACTATCGGCCATGAc              >  2:4828863/1‑150 (MQ=255)
                                                                                                                                   attGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGAc              <  2:4566448/150‑1 (MQ=255)
                                                                                                                                    ttGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACa             >  2:1372411/1‑150 (MQ=255)
                                                                                                                                    ttGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACa             >  2:4021792/1‑150 (MQ=255)
                                                                                                                                     tGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACAt            >  1:217081/1‑150 (MQ=255)
                                                                                                                                      ggCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACAtt           >  1:3309473/1‑150 (MQ=255)
                                                                                                                                      ggCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACAtt           >  1:5007596/1‑150 (MQ=255)
                                                                                                                                        cagcaCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTaa         >  1:1742259/1‑150 (MQ=255)
                                                                                                                                         agcaCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTaaa        >  2:3827698/1‑150 (MQ=255)
                                                                                                                                         agcaCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTaaa        >  1:3187882/1‑150 (MQ=255)
                                                                                                                                           caCTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAgg      <  1:3381542/150‑1 (MQ=255)
                                                                                                                                               gTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTc  >  2:2034856/1‑150 (MQ=255)
                                                                                                                                               gTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTc  >  1:256385/1‑150 (MQ=255)
                                                                                                                                               gTCTTTTATTGGTCTTTATTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTATGAACTTATTCAGAAAAACGTAGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTc  >  1:254593/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTC  >  CP009273/868354‑868646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCACT  >  CP009273/868351‑868649
                                                                                                                                                     |                                                                                                                                                     
GGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCT                                                                                                                                                       <  SRR19973063.4064444/150‑1 (MQ=60)
GGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCT                                                                                                                                                       <  SRR19973063.4064279/150‑1 (MQ=60)
   AATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTT                                                                                                                                                    >  SRR19973063.4479986/1‑150 (MQ=60)
      TTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATT                                                                                                                                                 <  SRR19973063.2253825/150‑1 (MQ=60)
       TGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTG                                                                                                                                                >  SRR19973063.2722606/1‑150 (MQ=60)
        GGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGG                                                                                                                                               >  SRR19973063.2897428/1‑150 (MQ=60)
        GGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGG                                                                                                                                               >  SRR19973063.2897579/1‑150 (MQ=60)
           TAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCT                                                                                                                                            <  SRR19973063.830917/150‑1 (MQ=60)
             AAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTT                                                                                                                                          >  SRR19973063.3374467/1‑150 (MQ=60)
                AGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTT                                                                                                                                       >  SRR19973063.5048880/1‑150 (MQ=60)
                AGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTT                                                                                                                                       <  SRR19973063.1129334/150‑1 (MQ=60)
                AGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTT                                                                                                                                       <  SRR19973063.2599406/150‑1 (MQ=60)
                 GCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTT                                                                                                                                      <  SRR19973063.4023799/150‑1 (MQ=60)
                    GCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCAT                                                                                                                                   <  SRR19973063.3999603/150‑1 (MQ=60)
                        CAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATT                                                                                                                               <  SRR19973063.1224097/150‑1 (MQ=60)
                        CAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATT                                                                                                                               <  SRR19973063.1123131/150‑1 (MQ=60)
                         AAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTACATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTA                                                                                                                              <  SRR19973063.4542561/150‑1 (MQ=60)
                           GGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAAC                                                                                                                            <  SRR19973063.3653985/150‑1 (MQ=60)
                                AATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCA                                                                                                                       <  SRR19973063.2973033/150‑1 (MQ=60)
                                AATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCA                                                                                                                       <  SRR19973063.2973214/150‑1 (MQ=60)
                                 ATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAA                                                                                                                      >  SRR19973063.1202139/1‑150 (MQ=60)
                                   TTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACT                                                                                                                    <  SRR19973063.3637402/150‑1 (MQ=60)
                                    TTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTG                                                                                                                   <  SRR19973063.84749/150‑1 (MQ=60)
                                      TGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGT                                                                                                                 >  SRR19973063.3299117/1‑150 (MQ=60)
                                      TGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGT                                                                                                                 <  SRR19973063.1155701/150‑1 (MQ=60)
                                        CAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTAT                                                                                                               >  SRR19973063.2415957/1‑150 (MQ=60)
                                         AGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATC                                                                                                              <  SRR19973063.3729571/150‑1 (MQ=60)
                                         AGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATC                                                                                                              >  SRR19973063.294976/1‑150 (MQ=60)
                                          GCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCG                                                                                                             <  SRR19973063.1636266/150‑1 (MQ=60)
                                            GGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGA                                                                                                           >  SRR19973063.510415/1‑150 (MQ=60)
                                               ACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCG                                                                                                        >  SRR19973063.2167820/1‑150 (MQ=60)
                                                 AGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCG                                                                                                      >  SRR19973063.4130297/1‑150 (MQ=60)
                                                    GTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGG                                                                                                   <  SRR19973063.108580/150‑1 (MQ=60)
                                                      TCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTA                                                                                                 >  SRR19973063.947281/1‑150 (MQ=60)
                                                          ATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAAC                                                                                             <  SRR19973063.4145870/150‑1 (MQ=60)
                                                          ATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAAC                                                                                             <  SRR19973063.1708888/150‑1 (MQ=60)
                                                          ATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAAC                                                                                             <  SRR19973063.1708867/150‑1 (MQ=60)
                                                                ATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCG                                                                                       <  SRR19973063.4725199/150‑1 (MQ=60)
                                                                    TGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAG                                                                                   <  SRR19973063.1986190/150‑1 (MQ=60)
                                                                      TTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCC                                                                                 <  SRR19973063.5072968/150‑1 (MQ=60)
                                                                           TGTATTATCTTGCTGTGTTTATACGAAAAGATAAAGTTTAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATAATTAAATATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTT                                                                            <  SRR19973063.3003241/150‑1 (MQ=60)
                                                                                     TGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTC                                                                  <  SRR19973063.3837133/150‑1 (MQ=60)
                                                                                     TGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTC                                                                  <  SRR19973063.4327459/150‑1 (MQ=60)
                                                                                     TGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTC                                                                  <  SRR19973063.1248818/150‑1 (MQ=60)
                                                                                      GCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCA                                                                 >  SRR19973063.3969428/1‑150 (MQ=60)
                                                                                        TNAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGA                                                               >  SRR19973063.1871571/1‑150 (MQ=60)
                                                                                         GAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAA                                                              >  SRR19973063.277015/1‑150 (MQ=60)
                                                                                         GAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAA                                                              >  SRR19973063.1286146/1‑150 (MQ=60)
                                                                                         GAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAA                                                              >  SRR19973063.3321944/1‑150 (MQ=60)
                                                                                          AGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAA                                                             >  SRR19973063.2925698/1‑150 (MQ=60)
                                                                                          AGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATAGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAA                                                             >  SRR19973063.2926029/1‑150 (MQ=60)
                                                                                              TATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACG                                                         <  SRR19973063.3956672/150‑1 (MQ=60)
                                                                                                TACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTT                                                       <  SRR19973063.725784/150‑1 (MQ=60)
                                                                                                TAAGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCATCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTT                                                       <  SRR19973063.725822/150‑1 (MQ=60)
                                                                                                 ACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTG                                                      <  SRR19973063.2884583/150‑1 (MQ=60)
                                                                                                 ACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTG                                                      <  SRR19973063.4017238/150‑1 (MQ=60)
                                                                                                 ACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTG                                                      <  SRR19973063.2884715/150‑1 (MQ=60)
                                                                                                   GAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGC                                                    >  SRR19973063.1091644/1‑150 (MQ=60)
                                                                                                   GAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGC                                                    >  SRR19973063.3765570/1‑150 (MQ=60)
                                                                                                    AAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCT                                                   <  SRR19973063.208210/150‑1 (MQ=60)
                                                                                                       AGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATT                                                <  SRR19973063.4151405/150‑1 (MQ=60)
                                                                                                          TAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCT                                             <  SRR19973063.2243961/150‑1 (MQ=60)
                                                                                                          TAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCT                                             <  SRR19973063.4722934/150‑1 (MQ=60)
                                                                                                             AGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTA                                          >  SRR19973063.1244911/1‑150 (MQ=60)
                                                                                                             AGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTA                                          >  SRR19973063.4354953/1‑150 (MQ=60)
                                                                                                              GATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAA                                         >  SRR19973063.4549626/1‑150 (MQ=60)
                                                                                                                  AGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGAT                                     >  SRR19973063.2935807/1‑150 (MQ=60)
                                                                                                                   GGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATA                                    >  SRR19973063.3725162/1‑150 (MQ=60)
                                                                                                                   GGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATA                                    >  SRR19973063.3724929/1‑150 (MQ=60)
                                                                                                                   GGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATA                                    >  SRR19973063.672936/1‑150 (MQ=60)
                                                                                                                    GCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCGACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATAT                                   >  SRR19973063.1237837/1‑150 (MQ=60)
                                                                                                                       GATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGA                                >  SRR19973063.3705751/1‑150 (MQ=60)
                                                                                                                             TTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTC                          >  SRR19973063.3758572/1‑150 (MQ=60)
                                                                                                                              TTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGTTATCGGAGCTCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTTAACGTTCG                         >  SRR19973063.2312703/1‑150 (MQ=60)
                                                                                                                              TTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCG                         >  SRR19973063.2312704/1‑150 (MQ=60)
                                                                                                                                ATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGC                       >  SRR19973063.4402226/1‑150 (MQ=60)
                                                                                                                                  TTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCC                     <  SRR19973063.224872/150‑1 (MQ=60)
                                                                                                                                  TTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCC                     <  SRR19973063.2652215/150‑1 (MQ=60)
                                                                                                                                        TGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACAT               >  SRR19973063.217735/1‑150 (MQ=60)
                                                                                                                                         GGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATT              >  SRR19973063.5023763/1‑150 (MQ=60)
                                                                                                                                         GGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATT              >  SRR19973063.3320060/1‑150 (MQ=60)
                                                                                                                                           CAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAA            >  SRR19973063.1747555/1‑150 (MQ=60)
                                                                                                                                            AGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAA           >  SRR19973063.3198136/1‑150 (MQ=60)
                                                                                                                                              CACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGG         <  SRR19973063.4512030/150‑1 (MQ=60)
                                                                                                                                              CACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGG         <  SRR19973063.3392321/150‑1 (MQ=60)
                                                                                                                                               ACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGA        >  SRR19973063.180323/1‑150 (MQ=60)
                                                                                                                                                  GTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTC     >  SRR19973063.3801590/1‑150 (MQ=60)
                                                                                                                                                  GTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTC     >  SRR19973063.257154/1‑150 (MQ=60)
                                                                                                                                                  GTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTC     >  SRR19973063.255401/1‑150 (MQ=60)
                                                                                                                                                  GTCTTTTATTGGTCTTTATTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTATGAACTTATTCAGAAAAACGTAGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTC     >  SRR19973063.255360/1‑150 (MQ=60)
                                                                                                                                                    CTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCAC   <  SRR19973063.2064453/150‑1 (MQ=60)
                                                                                                                                                     TTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCACT  >  SRR19973063.2243840/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGTAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCACT  >  CP009273/868351‑868649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑