Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 868,500 A→T 100% S22S (TCA→TCT)  yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273868,5000AT98.0% 644.3 / 2.1 202S22S (TCA→TCT) yliEputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base T (100/98);  total (102/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

AATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACT  >  CP009273/868354‑868645
                                                                                                                                                  |                                                                                                                                                  
aaTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCttt                                                                                                                                                  <  2:2586129/149‑1 (MQ=255)
aaTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCttt                                                                                                                                                  <  2:918432/149‑1 (MQ=255)
aaTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCttt                                                                                                                                                  <  2:2587638/149‑1 (MQ=255)
 aTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                                 <  1:1099954/149‑1 (MQ=255)
 aTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                                 >  1:1745328/1‑149 (MQ=255)
 aTTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCtttt                                                                                                                                                 <  2:3909017/149‑1 (MQ=255)
  tttGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTa                                                                                                                                                <  2:4073015/149‑1 (MQ=255)
    tGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGAACTGTCTTTTAtt                                                                                                                                              >  1:1014257/1‑149 (MQ=255)
     ggTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTg                                                                                                                                             <  1:804914/149‑1 (MQ=255)
       ttAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGt                                                                                                                                           >  2:197001/1‑149 (MQ=255)
        tAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTc                                                                                                                                          <  1:821311/149‑1 (MQ=255)
          aaaaGCCGCAGCAAGGGACAATTTTTGCAGCGGCATAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑tt                                                                                                                                       <  2:3165720/149‑1 (MQ=255)
           aaaGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑ttt                                                                                                                                      <  2:1692016/149‑1 (MQ=255)
            aaGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑tttt                                                                                                                                     <  1:3556955/149‑1 (MQ=255)
            aaGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑tttt                                                                                                                                     <  2:729026/149‑1 (MQ=255)
            aaGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑tttt                                                                                                                                     <  1:3073795/149‑1 (MQ=255)
             aGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑ttttt                                                                                                                                    <  2:1236744/149‑1 (MQ=255)
              gCCGCAGCAAGTGACAATTTTTTCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAAGATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑tttttt                                                                                                                                   <  2:1934396/149‑1 (MQ=255)
              gCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑tttttt                                                                                                                                   <  1:1444833/149‑1 (MQ=255)
                cGCAGCAAGGGACAATTTGTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTtc                                                                                                                                 <  2:1012681/149‑1 (MQ=255)
                 gcagcaAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTtca                                                                                                                                <  2:2139330/149‑1 (MQ=255)
                 gcagcaAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTtca                                                                                                                                >  1:3041460/1‑149 (MQ=255)
                   agcaAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTtcatc                                                                                                                              >  2:3575010/1‑149 (MQ=255)
                       aGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCTCTGTGTTTTATTGGTC‑TTTTTTTCTTCAttt                                                                                                                          >  1:3982765/1‑148 (MQ=255)
                         ggACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAAc                                                                                                                        <  1:2476654/149‑1 (MQ=255)
                           aCAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTa                                                                                                                      >  1:1356052/1‑149 (MQ=255)
                             aaTTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTTGCAGAACTGTCTTTTATTGGTA‑TTTTTTTCATCATTAACTATc                                                                                                                    >  1:4058051/1‑149 (MQ=255)
                             aaTTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATc                                                                                                                    >  1:3362059/1‑149 (MQ=255)
                               tttttGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCaa                                                                                                                  <  2:2472052/149‑1 (MQ=255)
                                 tttGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACt                                                                                                                >  2:1571951/1‑149 (MQ=255)
                                          gCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGa                                                                                                       <  2:3028415/149‑1 (MQ=255)
                                               gCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTGTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAgcgcg                                                                                                  >  1:2389547/1‑149 (MQ=255)
                                                 gTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGcgcgcg                                                                                                <  2:1343400/149‑1 (MQ=255)
                                                 gTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGcgcgcg                                                                                                <  1:23588/149‑1 (MQ=255)
                                                   tCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGt                                                                                              <  1:3782473/149‑1 (MQ=255)
                                                           ttATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTg                                                                                      <  2:3819126/149‑1 (MQ=255)
                                                            tATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGc                                                                                     <  1:2248023/149‑1 (MQ=255)
                                                            tATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGc                                                                                     <  1:1315059/149‑1 (MQ=255)
                                                            tATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGc                                                                                     >  1:724752/1‑149 (MQ=255)
                                                             aTTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGcc                                                                                    >  1:251452/1‑149 (MQ=255)
                                                              ttttGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTGTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCg                                                                                   >  1:1071313/1‑149 (MQ=255)
                                                                  gTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAg                                                                               <  2:399768/149‑1 (MQ=255)
                                                                    tAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGcc                                                                             <  1:2801873/149‑1 (MQ=255)
                                                                    tAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGcc                                                                             >  1:1723242/1‑149 (MQ=255)
                                                                      aaTGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGc                                                                           >  1:3322075/1‑149 (MQ=255)
                                                                       aTGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCt                                                                          <  2:1503479/149‑1 (MQ=255)
                                                                          tATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTg                                                                       >  1:2340090/1‑149 (MQ=255)
                                                                          tATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTg                                                                       >  1:2018937/1‑149 (MQ=255)
                                                                            ttAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGaa                                                                     >  2:3121033/1‑149 (MQ=255)
                                                                            ttAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGaa                                                                     <  2:3240654/149‑1 (MQ=255)
                                                                                  tGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTAtt                                                               <  2:757644/149‑1 (MQ=255)
                                                                                     tGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAg                                                            >  2:681796/1‑149 (MQ=255)
                                                                                     tGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTA‑TTTTTTTCATCATTAAATATCAACTGTTATCTGATCGCCCGGTAAAACGTGACGATAGCCGCTTTGAACTTATTCAg                                                            >  2:582435/1‑149 (MQ=255)
                                                                                         tttATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGaaaa                                                        >  2:572685/1‑149 (MQ=255)
                                                                                            ataCGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACg                                                     <  1:2970033/149‑1 (MQ=255)
                                                                                                gAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTgg                                                 >  2:2676/1‑149 (MQ=255)
                                                                                                 aaaaGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGc                                                <  2:620611/149‑1 (MQ=255)
                                                                                                     tataaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTAtt                                            >  1:1261071/2‑149 (MQ=255)
                                                                                                     gataaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTAtt                                            <  2:3708816/149‑1 (MQ=255)
                                                                                                      ataaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTAttt                                           <  2:483695/149‑1 (MQ=255)
                                                                                                       taaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTc                                          >  2:549013/1‑149 (MQ=255)
                                                                                                       taaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTc                                          <  2:3476906/149‑1 (MQ=255)
                                                                                                        aaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCt                                         >  2:2776034/1‑149 (MQ=255)
                                                                                                        aaagataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCt                                         <  1:2584458/149‑1 (MQ=255)
                                                                                                          agataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCttt                                       <  2:1745328/149‑1 (MQ=255)
                                                                                                          agataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCttt                                       >  1:3214739/1‑149 (MQ=255)
                                                                                                            ataaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTaa                                     >  1:1308900/1‑149 (MQ=255)
                                                                                                             taaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTaaa                                    <  1:527428/149‑1 (MQ=255)
                                                                                                             taaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTaaa                                    <  1:2480124/149‑1 (MQ=255)
                                                                                                              aaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAg                                   >  1:4101633/1‑149 (MQ=255)
                                                                                                              aaGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAg                                   >  1:2100938/1‑149 (MQ=255)
                                                                                                                ggCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGat                                 >  1:3144677/1‑149 (MQ=255)
                                                                                                                  cTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGatat                               <  2:1986126/149‑1 (MQ=255)
                                                                                                                    gATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTg                             >  1:661032/1‑149 (MQ=255)
                                                                                                                     aTAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGa                            >  2:312836/1‑149 (MQ=255)
                                                                                                                      tAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGaa                           <  1:917133/149‑1 (MQ=255)
                                                                                                                      tAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAAATGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAAAGTTGGCTATTTCTTTAAAGATATTGaa                           >  1:1969082/1‑149 (MQ=255)
                                                                                                                       aaTTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAAc                          >  1:3720826/1‑149 (MQ=255)
                                                                                                                         ttttatttttatTGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGt                        <  1:1169459/149‑1 (MQ=255)
                                                                                                                          tttatttttattGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGtt                       <  2:3322075/149‑1 (MQ=255)
                                                                                                                           ttatttttattGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTc                      >  2:3004076/1‑149 (MQ=255)
                                                                                                                            tatttttattGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCTGAGCGCGCGGTAAAACGTGCCTATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAAAGttag                     >  2:132875/1‑147 (MQ=255)
                                                                                                                            tattgttattGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAAATGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTTAACTTATTCAGAAAAACGTTGTCTATTTCTTTGAAGATATTGAACGTTCg                     >  1:715279/1‑149 (MQ=255)
                                                                                                                                tttattGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGccc                 <  2:360305/149‑1 (MQ=255)
                                                                                                                                tttattGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGccc                 >  1:623462/1‑149 (MQ=255)
                                                                                                                                tttattGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGccc                 <  2:2431187/149‑1 (MQ=255)
                                                                                                                                tttattGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGccc                 >  1:2688606/1‑149 (MQ=255)
                                                                                                                                 ttattGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCt                <  2:1056657/149‑1 (MQ=255)
                                                                                                                                  tattGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTg               >  2:3878511/1‑149 (MQ=255)
                                                                                                                                   attGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGa              >  2:2596003/1‑149 (MQ=255)
                                                                                                                                   attGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGa              >  1:1820370/1‑149 (MQ=255)
                                                                                                                                    ttGGCAGCACTGTCTTTTATTGGTCTTTTTTTTCAACATTAACAATAAAGTGTTATCGGAGCGCGCGGTAAAACTTGCCGATAGCCGCTTTGAACTAATACAGAAAAACGTTGGCTATTTCTTTAAATATATTTAACGTTCGGCCCTGa              >  2:3201733/1‑149 (MQ=255)
                                                                                                                                     tGGCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACa            <  1:34008/149‑1 (MQ=255)
                                                                                                                                      ggCAGCACTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACAt           <  1:3396571/149‑1 (MQ=255)
                                                                                                                                         agcaCTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTaa        <  2:1603505/149‑1 (MQ=255)
                                                                                                                                          gcaCTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTaaa       <  2:3041460/149‑1 (MQ=255)
                                                                                                                                           caCTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAg      <  2:3420871/149‑1 (MQ=255)
                                                                                                                                            aCTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAgg     >  1:2066456/1‑149 (MQ=255)
                                                                                                                                            aCTGTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAgg     >  1:3753477/1‑149 (MQ=255)
                                                                                                                                               gTCTTTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACt  <  1:2747455/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTC‑TTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACT  >  CP009273/868354‑868645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCACT  >  CP009273/868353‑868649
                                                                                                                                                   |                                                                                                                                                     
TAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTT                                                                                                                                                     <  SRR19973068.3704269/150‑1 (MQ=60)
 AATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTT                                                                                                                                                    <  SRR19973068.3921212/150‑1 (MQ=60)
 AATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTT                                                                                                                                                    >  SRR19973068.1756453/1‑150 (MQ=60)
 AATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTT                                                                                                                                                    >  SRR19973068.1244896/1‑150 (MQ=60)
  ATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTA                                                                                                                                                   <  SRR19973068.1107280/150‑1 (MQ=60)
   TTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTAT                                                                                                                                                  <  SRR19973068.2286064/150‑1 (MQ=60)
    TTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGAACTGTCTTTTATT                                                                                                                                                 >  SRR19973068.1020849/1‑150 (MQ=60)
      GGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGG                                                                                                                                               <  SRR19973068.810391/150‑1 (MQ=60)
         TAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCT                                                                                                                                            <  SRR19973068.826890/150‑1 (MQ=60)
            AAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTT                                                                                                                                         >  SRR19973068.130375/1‑150 (MQ=60)
             AAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTT                                                                                                                                        <  SRR19973068.3578565/150‑1 (MQ=60)
             AAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTT                                                                                                                                        <  SRR19973068.3092105/150‑1 (MQ=60)
             AAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTT                                                                                                                                        <  SRR19973068.3092556/150‑1 (MQ=60)
              AGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTT                                                                                                                                       <  SRR19973068.4120161/150‑1 (MQ=60)
              AGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTT                                                                                                                                       >  SRR19973068.487093/1‑150 (MQ=60)
               GCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTT                                                                                                                                      <  SRR19973068.1454305/150‑1 (MQ=60)
                 CGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCA                                                                                                                                    >  SRR19973068.3060115/1‑150 (MQ=60)
                       AAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCTCTGTGTTTTATTGGTCTTTTTTTCTTCATTT                                                                                                                              >  SRR19973068.4006842/1‑150 (MQ=60)
                         GGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAAC                                                                                                                            >  SRR19973068.761225/1‑150 (MQ=60)
                          GGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACT                                                                                                                           <  SRR19973068.2492454/150‑1 (MQ=60)
                          GGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACT                                                                                                                           <  SRR19973068.2493288/150‑1 (MQ=60)
                           GACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTA                                                                                                                          >  SRR19973068.2522582/1‑150 (MQ=60)
                           GACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTA                                                                                                                          >  SRR19973068.1364761/1‑150 (MQ=60)
                             CAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTTGCAGAACTGTCTTTTATTGGTATTTTTTTCATCATTAACTATC                                                                                                                        >  SRR19973068.4082456/1‑150 (MQ=60)
                             CAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATC                                                                                                                        >  SRR19973068.4082515/1‑150 (MQ=60)
                             CAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATC                                                                                                                        >  SRR19973068.3382503/1‑150 (MQ=60)
                                TTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAAC                                                                                                                     <  SRR19973068.3416764/150‑1 (MQ=60)
                                 TTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACT                                                                                                                    <  SRR19973068.3780732/150‑1 (MQ=60)
                                   TTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGG                                                                                                                  <  SRR19973068.2967612/150‑1 (MQ=60)
                                       AGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATC                                                                                                              <  SRR19973068.3420744/150‑1 (MQ=60)
                                               AGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTGTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCG                                                                                                      >  SRR19973068.2404778/1‑150 (MQ=60)
                                                  GTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTTAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGG                                                                                                   <  SRR19973068.24060/150‑1 (MQ=60)
                                                  GTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGG                                                                                                   <  SRR19973068.23927/150‑1 (MQ=60)
                                                    TCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTA                                                                                                 <  SRR19973068.3805044/150‑1 (MQ=60)
                                                    TCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTA                                                                                                 <  SRR19973068.2296596/150‑1 (MQ=60)
                                                         TAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACG                                                                                            <  SRR19973068.312589/150‑1 (MQ=60)
                                                            TTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGC                                                                                         >  SRR19973068.729507/1‑150 (MQ=60)
                                                            TTATTATGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATGTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGC                                                                                         >  SRR19973068.2360652/1‑150 (MQ=60)
                                                             TATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCC                                                                                        >  SRR19973068.253271/1‑150 (MQ=60)
                                                             TATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCC                                                                                        <  SRR19973068.1323648/150‑1 (MQ=60)
                                                             TATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCC                                                                                        <  SRR19973068.2262668/150‑1 (MQ=60)
                                                             TATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCC                                                                                        <  SRR19973068.1320996/150‑1 (MQ=60)
                                                             TATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCC                                                                                        >  SRR19973068.117943/1‑150 (MQ=60)
                                                              ATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCG                                                                                       >  SRR19973068.1078339/1‑150 (MQ=60)
                                                              ATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTGTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCG                                                                                       >  SRR19973068.1078363/1‑150 (MQ=60)
                                                                    TTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCC                                                                                 >  SRR19973068.1734192/1‑150 (MQ=60)
                                                                     TAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCG                                                                                <  SRR19973068.2819127/150‑1 (MQ=60)
                                                                     TAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCG                                                                                <  SRR19973068.2819281/150‑1 (MQ=60)
                                                                      AAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGC                                                                               >  SRR19973068.3342361/1‑150 (MQ=60)
                                                                      AAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGC                                                                               >  SRR19973068.664064/1‑150 (MQ=60)
                                                                      AAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGC                                                                               >  SRR19973068.599867/1‑150 (MQ=60)
                                                                      AAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGC                                                                               >  SRR19973068.3002238/1‑150 (MQ=60)
                                                                       AATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCT                                                                              <  SRR19973068.1751341/150‑1 (MQ=60)
                                                                         TGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTT                                                                            <  SRR19973068.3292767/150‑1 (MQ=60)
                                                                          GTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTG                                                                           >  SRR19973068.2032184/1‑150 (MQ=60)
                                                                          GTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTG                                                                           >  SRR19973068.2355121/1‑150 (MQ=60)
                                                                             TTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAAC                                                                        <  SRR19973068.1769856/150‑1 (MQ=60)
                                                                                 CATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTAT                                                                    >  SRR19973068.1897230/1‑150 (MQ=60)
                                                                                     CTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAG                                                                <  SRR19973068.815291/150‑1 (MQ=60)
                                                                                        AGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAA                                                             >  SRR19973068.3685006/1‑150 (MQ=60)
                                                                                         GTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAA                                                            >  SRR19973068.4067603/1‑150 (MQ=60)
                                                                                             ATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGT                                                        <  SRR19973068.2988430/150‑1 (MQ=60)
                                                                                                     ATATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATT                                                >  SRR19973068.1269399/1‑150 (MQ=60)
                                                                                                     AGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATT                                                >  SRR19973068.1617304/1‑150 (MQ=60)
                                                                                                         AAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTT                                            <  SRR19973068.2600787/150‑1 (MQ=60)
                                                                                                          AAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTT                                           >  SRR19973068.3234505/1‑150 (MQ=60)
                                                                                                          AAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTT                                           >  SRR19973068.1105448/1‑150 (MQ=60)
                                                                                                            GATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAA                                         >  SRR19973068.1317465/1‑150 (MQ=60)
                                                                                                            GATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAA                                         <  SRR19973068.1330915/150‑1 (MQ=60)
                                                                                                              TAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAG                                       >  SRR19973068.4126196/1‑150 (MQ=60)
                                                                                                              TAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAG                                       <  SRR19973068.531064/150‑1 (MQ=60)
                                                                                                              TAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAG                                       <  SRR19973068.2495941/150‑1 (MQ=60)
                                                                                                              TAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAG                                       >  SRR19973068.2114633/1‑150 (MQ=60)
                                                                                                                AGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGAT                                     >  SRR19973068.15679/1‑150 (MQ=60)
                                                                                                                AGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGAT                                     >  SRR19973068.826917/1‑150 (MQ=60)
                                                                                                                AGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGAT                                     >  SRR19973068.3164018/1‑150 (MQ=60)
                                                                                                                AGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGAT                                     >  SRR19973068.3163984/1‑150 (MQ=60)
                                                                                                                AGGCTGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGAT                                     >  SRR19973068.2108328/1‑150 (MQ=60)
                                                                                                                    TGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTG                                 >  SRR19973068.665284/1‑150 (MQ=60)
                                                                                                                    TGATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTG                                 >  SRR19973068.2740315/1‑150 (MQ=60)
                                                                                                                      ATAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAAATGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAAAGTTGGCTATTTCTTTAAAGATATTGAA                               >  SRR19973068.1982133/1‑150 (MQ=60)
                                                                                                                       TAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAAC                              <  SRR19973068.729387/150‑1 (MQ=60)
                                                                                                                       TAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAAC                              <  SRR19973068.923308/150‑1 (MQ=60)
                                                                                                                       TAATTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAAC                              >  SRR19973068.3743171/1‑150 (MQ=60)
                                                                                                                          TTTTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTT                           <  SRR19973068.1177273/150‑1 (MQ=60)
                                                                                                                            TTATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCG                         <  SRR19973068.2030803/150‑1 (MQ=60)
                                                                                                                            TTATTGTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAAATGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTTAACTTATTCAGAAAAACGTTGTCTATTTCTTTGAAGATATTGAACGTTCG                         >  SRR19973068.719929/1‑150 (MQ=60)
                                                                                                                             TATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTTAACGTTAGG                        >  SRR19973068.86692/1‑150 (MQ=60)
                                                                                                                             TATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGG                        >  SRR19973068.3347464/1‑150 (MQ=60)
                                                                                                                              ATTTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGC                       >  SRR19973068.1403935/1‑150 (MQ=60)
                                                                                                                                TTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCTGCCC                     >  SRR19973068.2705331/1‑150 (MQ=60)
                                                                                                                                TTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCC                     >  SRR19973068.2705338/1‑150 (MQ=60)
                                                                                                                                TTTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCC                     >  SRR19973068.627596/1‑150 (MQ=60)
                                                                                                                                 TTTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCT                    <  SRR19973068.3400441/150‑1 (MQ=60)
                                                                                                                                  TTATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTATTTAAAGATATTGAACGTTCGGCCCTG                   <  SRR19973068.2531659/150‑1 (MQ=60)
                                                                                                                                   TATTGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGA                  >  SRR19973068.1832333/1‑150 (MQ=60)
                                                                                                                                      TGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACAT               <  SRR19973068.34510/150‑1 (MQ=60)
                                                                                                                                      TGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACAT               <  SRR19973068.34447/150‑1 (MQ=60)
                                                                                                                                      TGGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAAAGTTCGGCCCTGACAT               >  SRR19973068.2134531/1‑150 (MQ=60)
                                                                                                                                       GGCAGCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATT              <  SRR19973068.3417135/150‑1 (MQ=60)
                                                                                                                                           GCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAG          >  SRR19973068.2723675/1‑150 (MQ=60)
                                                                                                                                           GCACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAG          <  SRR19973068.687038/150‑1 (MQ=60)
                                                                                                                                            CACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGG         >  SRR19973068.3775932/1‑150 (MQ=60)
                                                                                                                                            CACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGG         >  SRR19973068.3775871/1‑150 (MQ=60)
                                                                                                                                            CACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGG         >  SRR19973068.2079868/1‑150 (MQ=60)
                                                                                                                                            CACTGTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGG         >  SRR19973068.2079665/1‑150 (MQ=60)
                                                                                                                                             ACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGA        <  SRR19973068.2450277/150‑1 (MQ=60)
                                                                                                                                                GTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTC     >  SRR19973068.984129/1‑150 (MQ=60)
                                                                                                                                                GTCTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTC     <  SRR19973068.2764352/150‑1 (MQ=60)
                                                                                                                                                  CTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCAC   >  SRR19973068.1942405/1‑150 (MQ=60)
                                                                                                                                                  CTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCAC   >  SRR19973068.3470848/1‑150 (MQ=60)
                                                                                                                                                  CTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCAC   >  SRR19973068.3470803/1‑150 (MQ=60)
                                                                                                                                                  CTTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCAC   >  SRR19973068.3470778/1‑150 (MQ=60)
                                                                                                                                                   TTTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCACT  <  SRR19973068.1118179/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TAATTTGGTTAAAAAGCCGCAGCAAGGGACAATTTTTGCAGCGGCACAGCGTTCAGATAGTTATTTTGTTAAATGTATTAACATGCTGAGTTTATACGAAAAGATAAAGATAAGGCTGATAATTTTATTTTTATTGGCAGCACTGTCATTTATTGGTCTTTTTTTCATCATTAACTATCAACTGGTATCGGAGCGCGCGGTAAAACGTGCCGATAGCCGCTTTGAACTTATTCAGAAAAACGTTGGCTATTTCTTTAAAGATATTGAACGTTCGGCCCTGACATTAAAGGACTCACT  >  CP009273/868353‑868649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑