Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,335,356 A→T 93.5% H181L (CAT→CTT)  yciM → envelope integrity maintenance protein; EnvC‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,335,3560AT93.5% 648.5 / 23.5 210H181L (CAT→CTT) yciMenvelope integrity maintenance protein; EnvC‑interacting protein
Reads supporting (aligned to +/- strand):  ref base A (8/6);  new base T (99/97);  total (107/103)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

CTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGA  >  CP009273/1335211‑1335500
                                                                                                                                                 |                                                                                                                                                
cTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCttt                                                                                                                                                <  1:2458115/148‑1 (MQ=255)
  gACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTc                                                                                                                                              >  1:705648/1‑148 (MQ=255)
       aTGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACtg                                                                                                                                         >  1:906849/1‑148 (MQ=255)
        tGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACtgt                                                                                                                                        >  2:116383/1‑148 (MQ=255)
        tGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACtgt                                                                                                                                        >  2:114030/1‑148 (MQ=255)
         gAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACtgtg                                                                                                                                       <  1:2503247/148‑1 (MQ=255)
            aCTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGt                                                                                                                                    >  2:508135/1‑148 (MQ=255)
             cTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGtt                                                                                                                                   >  2:3391281/1‑148 (MQ=255)
              tGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTa                                                                                                                                  <  2:2329405/148‑1 (MQ=255)
                aCTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGc                                                                                                                                >  1:2124457/1‑148 (MQ=255)
                   tCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCt                                                                                                                             >  1:1372286/1‑148 (MQ=255)
                           ggCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCata                                                                                                                     <  2:1607059/148‑1 (MQ=255)
                           ggCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCata                                                                                                                     <  2:2255758/148‑1 (MQ=255)
                           ggCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCata                                                                                                                     <  2:3123617/148‑1 (MQ=255)
                            gcgcgcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCatat                                                                                                                    >  2:1005418/1‑148 (MQ=255)
                             cgcgcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATg                                                                                                                   >  1:3503167/1‑148 (MQ=255)
                             cgcgcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATg                                                                                                                   >  1:3502892/1‑148 (MQ=255)
                                 cTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCa                                                                                                               >  2:2518982/1‑148 (MQ=255)
                                       cAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGcgacg                                                                                                         >  2:2276593/1‑148 (MQ=255)
                                       cAGGTGCTAGAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTGGAAATTGCCCTTTTCTACTGGGAGTTAGCCCTGCAGCATATGGCCAGcgacg                                                                                                         <  2:1963325/148‑1 (MQ=255)
                                         gTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAt                                                                                                       <  2:2476918/148‑1 (MQ=255)
                                         gTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAt                                                                                                       <  1:2208532/148‑1 (MQ=255)
                                          ttGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtc                                                                                                      <  2:2514402/148‑1 (MQ=255)
                                               aCAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGAt                                                                                                 >  2:454303/1‑148 (MQ=255)
                                                cAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATc                                                                                                >  2:280422/1‑148 (MQ=255)
                                                cAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATc                                                                                                <  1:2518897/148‑1 (MQ=255)
                                                      tACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCa                                                                                          <  2:1189944/148‑1 (MQ=255)
                                                      tACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCa                                                                                          <  1:1870671/148‑1 (MQ=255)
                                                        ccAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATg                                                                                        <  2:3214708/148‑1 (MQ=255)
                                                        ccAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATg                                                                                        <  2:2518286/148‑1 (MQ=255)
                                                          aGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAc                                                                                      <  1:1177666/148‑1 (MQ=255)
                                                          aGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAc                                                                                      <  1:1177718/148‑1 (MQ=255)
                                                            gCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCt                                                                                    >  1:2511511/1‑148 (MQ=255)
                                                            gCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCt                                                                                    >  1:2778833/1‑148 (MQ=255)
                                                                ccAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCt                                                                                >  1:1947112/1‑148 (MQ=255)
                                                                  agtgagtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTaa                                                                              >  2:1691606/1‑148 (MQ=255)
                                                                  agtgagtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTaa                                                                              >  1:1219885/1‑148 (MQ=255)
                                                                    tgagtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTaaaa                                                                            >  2:2258786/1‑148 (MQ=255)
                                                                     gagtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTaaaaa                                                                           >  1:761576/1‑148 (MQ=255)
                                                                     gagtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTaaaaa                                                                           >  1:1169025/1‑148 (MQ=255)
                                                                      agaggCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTaaaaaa                                                                          <  1:568472/145‑1 (MQ=255)
                                                                        tgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAg                                                                        <  1:733373/148‑1 (MQ=255)
                                                                        tgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAg                                                                        <  1:1738541/148‑1 (MQ=255)
                                                                        tgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAg                                                                        >  1:1971273/1‑148 (MQ=255)
                                                                         ggCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAgg                                                                       <  2:3502892/148‑1 (MQ=255)
                                                                         ggCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGAATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAgg                                                                       <  1:2095543/148‑1 (MQ=255)
                                                                          gCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAggg                                                                      <  1:3230478/148‑1 (MQ=255)
                                                                          gCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAggg                                                                      <  1:3211845/148‑1 (MQ=255)
                                                                          gCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAggg                                                                      <  1:3006173/148‑1 (MQ=255)
                                                                           cAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAgggg                                                                     >  1:1386155/1‑148 (MQ=255)
                                                                           cAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAgggg                                                                     <  2:2873245/148‑1 (MQ=255)
                                                                           cAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAgggg                                                                     >  1:2525282/1‑148 (MQ=255)
                                                                                aGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGggcggcg                                                                >  2:214853/1‑148 (MQ=255)
                                                                                 gCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGggcggcgg                                                               <  1:649030/148‑1 (MQ=255)
                                                                                 gCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGggcggcgg                                                               <  1:2433108/148‑1 (MQ=255)
                                                                                  cAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGggcggcggc                                                              <  1:367702/148‑1 (MQ=255)
                                                                                    aTTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAg                                                            <  2:1334730/148‑1 (MQ=255)
                                                                                     ttGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGa                                                           <  1:299530/148‑1 (MQ=255)
                                                                                        aTGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATaa                                                        >  2:621376/1‑148 (MQ=255)
                                                                                          gTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATaaaa                                                      >  1:3522024/1‑148 (MQ=255)
                                                                                           ttGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATaaaaa                                                     <  2:34215/148‑1 (MQ=255)
                                                                                           ttGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATaaaaa                                                     <  2:35812/148‑1 (MQ=255)
                                                                                            tGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAAc                                                    <  1:2036203/148‑1 (MQ=255)
                                                                                             gCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACa                                                   <  2:3319829/148‑1 (MQ=255)
                                                                                             gCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATAGGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACa                                                   <  2:497521/148‑1 (MQ=255)
                                                                                                gAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAgcg                                                <  1:1913122/148‑1 (MQ=255)
                                                                                                 aaCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAgcgc                                               >  1:222292/1‑148 (MQ=255)
                                                                                                  aCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGcc                                              >  1:83976/1‑148 (MQ=255)
                                                                                                       tGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGt                                         >  2:3262113/1‑148 (MQ=255)
                                                                                                        ggTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTTCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTa                                        >  2:1912787/1‑148 (MQ=255)
                                                                                                        ggTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTa                                        >  2:2618679/1‑148 (MQ=255)
                                                                                                         gTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAt                                       >  2:470491/1‑148 (MQ=255)
                                                                                                          tGAATCTGGGTAAAGATAAACATCGCGTCGAAATTGCCCTTTTCTAATGTGAGTAAGCCCTGCAGCATATGGCCAGCGAAGATCTCGATCGAGCCATGACCTAGCTAAAAAAAGGGGCGGCGGCAGAGAAAAACAGCGCCAGCGTAta                                      >  2:1694953/1‑147 (MQ=255)
                                                                                                          tGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACGGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATc                                      <  2:705648/148‑1 (MQ=255)
                                                                                                           gAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATcc                                     >  1:1289132/1‑148 (MQ=255)
                                                                                                            aaGCTGGTTAAAGATAAACAGCGCTTCTAAATTGCCCTTTTCTACTGTGTTTTAGCCCTGCAGCATATGGCCAGCGACTATCTCGATCGTGCCATGACCTTGATAAAAAAAGGGGCGGCGGCTTATAAAAACAGCGCCCGCGTATCCa                                    <  1:2868768/148‑1 (MQ=255)
                                                                                                               cTGGGTAAAGATAACCCGCGCGTCGAAATTGCCCTTTTCTAGTGTGTTTTAGCCCTGCAGCATTGGGCCCGCGCCTATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGGGGCGGAAGATAAAAACCGCGCCCGCGTATCCATaa                                 <  2:1219885/148‑1 (MQ=255)
                                                                                                                     aaaGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATgg                           >  2:682465/1‑148 (MQ=255)
                                                                                                                     aaaGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATgg                           >  2:2761607/1‑148 (MQ=255)
                                                                                                                     aaaGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATgg                           >  2:139543/1‑148 (MQ=255)
                                                                                                                      aaGATAAACAGCGCGTCGAAATTGCCCTTTTATACTGTGTGTTAGCCCTGCAGAATATGGCCCGCGAGTATCTCTATCGTGCCTTGACCTTGCTAAAAAAAGGGGGGGCGGCATATAAAAACAGCGCCCGCGTATCCATAATGATggg                          <  1:1358716/148‑1 (MQ=255)
                                                                                                                       aGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGa                         >  2:1568325/1‑148 (MQ=255)
                                                                                                                       aGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGa                         >  1:3102392/1‑148 (MQ=255)
                                                                                                                        gATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGAc                        >  2:2398212/1‑148 (MQ=255)
                                                                                                                          tAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGAcgc                      >  1:1710917/1‑148 (MQ=255)
                                                                                                                             aCAGCGCGTCTAAATTGCCTTTTTCTACTGTGTGTTAGCCCTGCAGCTTATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAATGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCgtg                   <  1:3391281/148‑1 (MQ=255)
                                                                                                                              ccgcgcGTCGAAATTGCCGTTTTCTAGTGTTTGTTAGCCCTGCAGCATTTGGCCCGCGACTATATCGATCGTGCCATGACGTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACCGCGCCCGCGTATCCATAATGATGGGACGCgtgt                  <  1:1043038/146‑1 (MQ=255)
                                                                                                                              cAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCgtgt                  >  2:3184782/1‑148 (MQ=255)
                                                                                                                               cgcgcGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGtt                 <  1:2014344/147‑1 (MQ=255)
                                                                                                                               aGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGTTGGGACGCGTGtt                 >  2:2323958/1‑148 (MQ=255)
                                                                                                                               aGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATAGGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGtt                 <  2:3471180/148‑1 (MQ=255)
                                                                                                                                gcggGTCGAAATTGCCATTTACTACTGTTTTTTAGCCCTGCAGCATATGGCCAGCGACGATCTCTATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCATATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGttt                <  1:464993/148‑1 (MQ=255)
                                                                                                                                gcgcGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGttt                <  1:1477947/148‑1 (MQ=255)
                                                                                                                                gcgcGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCTTGACCTTGATAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGttt                <  1:1458754/148‑1 (MQ=255)
                                                                                                                                  gcgTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTAt              <  2:1724053/148‑1 (MQ=255)
                                                                                                                                   cgTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATg             >  2:2307661/1‑148 (MQ=255)
                                                                                                                                          aTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAg      >  2:451351/1‑148 (MQ=255)
                                                                                                                                          aTTGCCCATTTCTAGTGTGAGTTACCCCTGCAGCATAGGCCCAGCGACGATCTCGAGCGTGCCATGACCTTGAAAAAAAATGGGGCGGCGGCACATAAAAACCGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAg      <  1:114074/148‑1 (MQ=255)
                                                                                                                                            tGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGga    >  2:2581786/1‑148 (MQ=255)
                                                                                                                                              cccTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGgaga  >  2:3019748/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGA  >  CP009273/1335211‑1335500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAATACG  >  CP009273/1335208‑1335505
                                                                                                                                                    |                                                                                                                                                     
CAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTT                                                                                                                                                      <  SRR19973067.3042709/150‑1 (MQ=60)
  GCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTT                                                                                                                                                    <  SRR19973067.437882/150‑1 (MQ=60)
  GCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTT                                                                                                                                                    <  SRR19973067.437889/150‑1 (MQ=60)
   CTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTC                                                                                                                                                   <  SRR19973067.2474278/150‑1 (MQ=60)
   CTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTC                                                                                                                                                   >  SRR19973067.710529/1‑150 (MQ=60)
      ACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTAC                                                                                                                                                <  SRR19973067.2836492/150‑1 (MQ=60)
        CGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTG                                                                                                                                              >  SRR19973067.912850/1‑150 (MQ=60)
          ATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTG                                                                                                                                            <  SRR19973067.769608/150‑1 (MQ=60)
          ATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTG                                                                                                                                            >  SRR19973067.723665/1‑150 (MQ=60)
            GAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAG                                                                                                                                          <  SRR19973067.2519674/150‑1 (MQ=60)
                CTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAG                                                                                                                                      >  SRR19973067.2491637/1‑150 (MQ=60)
                CTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAG                                                                                                                                      >  SRR19973067.612126/1‑150 (MQ=60)
                CTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAG                                                                                                                                      >  SRR19973067.698679/1‑150 (MQ=60)
                 TGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGC                                                                                                                                     >  SRR19973067.2138632/1‑150 (MQ=60)
                  GACGTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCC                                                                                                                                    <  SRR19973067.3017452/150‑1 (MQ=60)
                    CTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCT                                                                                                                                  >  SRR19973067.1381653/1‑150 (MQ=60)
                        CGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAG                                                                                                                              <  SRR19973067.2316138/150‑1 (MQ=60)
                              GGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATG                                                                                                                        >  SRR19973067.3525246/1‑150 (MQ=60)
                              GGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATG                                                                                                                        >  SRR19973067.3525093/1‑150 (MQ=60)
                              GGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATG                                                                                                                        >  SRR19973067.3525368/1‑150 (MQ=60)
                                     TGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCG                                                                                                                 <  SRR19973067.2524324/150‑1 (MQ=60)
                                       CAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGAC                                                                                                               >  SRR19973067.951290/1‑150 (MQ=60)
                                        AACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACG                                                                                                              >  SRR19973067.1219947/1‑150 (MQ=60)
                                           AGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATC                                                                                                           >  SRR19973067.1735867/1‑150 (MQ=60)
                                            GTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCT                                                                                                          <  SRR19973067.2223258/150‑1 (MQ=60)
                                            GTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCT                                                                                                          <  SRR19973067.2223297/150‑1 (MQ=60)
                                                   CAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGT                                                                                                   <  SRR19973067.2535414/150‑1 (MQ=60)
                                                   CAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGT                                                                                                   <  SRR19973067.2535499/150‑1 (MQ=60)
                                                    AAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTG                                                                                                  >  SRR19973067.724404/1‑150 (MQ=60)
                                                    AAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTG                                                                                                  >  SRR19973067.2042740/1‑150 (MQ=60)
                                                     AATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGC                                                                                                 >  SRR19973067.2179293/1‑150 (MQ=60)
                                                         TACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATG                                                                                             <  SRR19973067.1883431/150‑1 (MQ=60)
                                                             AGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCT                                                                                         >  SRR19973067.2796779/1‑150 (MQ=60)
                                                             AGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCT                                                                                         >  SRR19973067.2527993/1‑150 (MQ=60)
                                                             AGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCT                                                                                         <  SRR19973067.1185503/150‑1 (MQ=60)
                                                             AGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCT                                                                                         <  SRR19973067.1185472/150‑1 (MQ=60)
                                                             AGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCT                                                                                         <  SRR19973067.1185451/150‑1 (MQ=60)
                                                                 TACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT                                                                                     >  SRR19973067.1960384/1‑150 (MQ=60)
                                                                  ACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTA                                                                                    >  SRR19973067.3052926/1‑150 (MQ=60)
                                                                  ACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTA                                                                                    >  SRR19973067.14859/1‑150 (MQ=60)
                                                                   CCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAA                                                                                   >  SRR19973067.1917635/1‑150 (MQ=60)
                                                                   CCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAA                                                                                   >  SRR19973067.1227998/1‑150 (MQ=60)
                                                                      GTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAA                                                                                >  SRR19973067.766865/1‑150 (MQ=60)
                                                                      GTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAA                                                                                >  SRR19973067.1176796/1‑150 (MQ=60)
                                                                      GTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAA                                                                                >  SRR19973067.1176782/1‑150 (MQ=60)
                                                                      GTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAA                                                                                >  SRR19973067.766611/1‑150 (MQ=60)
                                                                         AGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAG                                                                             >  SRR19973067.1107497/1‑150 (MQ=60)
                                                                         AGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAG                                                                             >  SRR19973067.1984639/1‑150 (MQ=60)
                                                                         AGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAG                                                                             >  SRR19973067.1984198/1‑150 (MQ=60)
                                                                         AGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAA                                                                             <  SRR19973067.13876/150‑1 (MQ=60)
                                                                         AGAGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAG                                                                             <  SRR19973067.572166/150‑1 (MQ=60)
                                                                           TGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGG                                                                           <  SRR19973067.738465/150‑1 (MQ=60)
                                                                           TGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGG                                                                           <  SRR19973067.1750428/150‑1 (MQ=60)
                                                                            GGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGG                                                                          >  SRR19973067.1395587/1‑150 (MQ=60)
                                                                            GGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGG                                                                          >  SRR19973067.1459418/1‑150 (MQ=60)
                                                                            GGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGG                                                                          >  SRR19973067.2541831/1‑150 (MQ=60)
                                                                            GGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGAATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGG                                                                          <  SRR19973067.2109409/150‑1 (MQ=60)
                                                                             GCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGC                                                                         <  SRR19973067.3250755/150‑1 (MQ=60)
                                                                             GCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGC                                                                         <  SRR19973067.3232636/150‑1 (MQ=60)
                                                                             GCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGC                                                                         <  SRR19973067.3232075/150‑1 (MQ=60)
                                                                             GCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGC                                                                         <  SRR19973067.3025273/150‑1 (MQ=60)
                                                                             GCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGC                                                                         <  SRR19973067.2530343/150‑1 (MQ=60)
                                                                             GCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGC                                                                         <  SRR19973067.2528396/150‑1 (MQ=60)
                                                                                 AAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCG                                                                     >  SRR19973067.1389687/1‑150 (MQ=60)
                                                                                    GCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCA                                                                  <  SRR19973067.2449081/150‑1 (MQ=60)
                                                                                    GCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCA                                                                  <  SRR19973067.653430/150‑1 (MQ=60)
                                                                                     CAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAG                                                                 <  SRR19973067.370258/150‑1 (MQ=60)
                                                                                     CAATTGATGTTGCCGAACGCCTGGTGAAGCGGGGTAAAGATAAACAGCGCGTCGAAAGTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAG                                                                 <  SRR19973067.369610/150‑1 (MQ=60)
                                                                                        TTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATA                                                              <  SRR19973067.301440/150‑1 (MQ=60)
                                                                                        TTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATA                                                              <  SRR19973067.3144996/150‑1 (MQ=60)
                                                                                          GATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAA                                                            >  SRR19973067.2044109/1‑150 (MQ=60)
                                                                                           ATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAA                                                           >  SRR19973067.1776223/1‑150 (MQ=60)
                                                                                           ATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAA                                                           >  SRR19973067.3544300/1‑150 (MQ=60)
                                                                                           ATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAA                                                           >  SRR19973067.1361077/1‑150 (MQ=60)
                                                                                               TGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAG                                                       <  SRR19973067.2049835/150‑1 (MQ=60)
                                                                                               TGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAG                                                       <  SRR19973067.2049907/150‑1 (MQ=60)
                                                                                                  CGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGC                                                    >  SRR19973067.223916/1‑150 (MQ=60)
                                                                                                   GAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCC                                                   <  SRR19973067.2123257/150‑1 (MQ=60)
                                                                                                   GAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCC                                                   <  SRR19973067.1926175/150‑1 (MQ=60)
                                                                                                   GAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCC                                                   >  SRR19973067.84892/1‑150 (MQ=60)
                                                                                                    AACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCC                                                  >  SRR19973067.295070/1‑150 (MQ=60)
                                                                                                          TGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAT                                            >  SRR19973067.2684449/1‑150 (MQ=60)
                                                                                                            GTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCC                                          >  SRR19973067.1297932/1‑150 (MQ=60)
                                                                                                               AAGCTGGTTAAAGATAAACAGCGCTTCTAAATTGCCCTTTTCTACTGTGTTTTAGCCCTGCAGCATATGGCCAGCGACTATCTCGATCGTGCCATGACCTTGATAAAAAAAGGGGCGGCGGCTTATAAAAACAGCGCCCGCGTATCCATA                                       <  SRR19973067.2887135/150‑1 (MQ=60)
                                                                                                                 GCTGGTTAAAGATAAACCGCGCGTCGAAATTGCCGTTTTGTACTGTGAGTTCGCCCGGCAGCATTTGGCCAGCGAGGATCTCGATCGTGCCATGACCTTGCTAAAAAATGGGGGGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAAT                                     <  SRR19973067.3537045/150‑1 (MQ=60)
                                                                                                                   TGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGA                                   >  SRR19973067.1926891/1‑150 (MQ=60)
                                                                                                                       TAAATATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCCGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGG                               <  SRR19973067.3282434/150‑1 (MQ=60)
                                                                                                                       TAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGTGTTAGCCCTGCAGCATATGGCCCGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGG                               <  SRR19973067.3282485/150‑1 (MQ=60)
                                                                                                                       TAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGG                               >  SRR19973067.3478288/1‑150 (MQ=60)
                                                                                                                        AAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGA                              >  SRR19973067.3122127/1‑150 (MQ=60)
                                                                                                                         AAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGAC                             >  SRR19973067.142300/1‑150 (MQ=60)
                                                                                                                         AAGATAAACAGCGCGTCGAAATTGCCCTTTTATACTGTGTGTTAGCCCTGCAGAATATGGCCCGCGAGTATCTCTATCGTGCCTTGACCTTGCTAAAAAAAGGGGGGGCGGCATATAAAAACAGCGCCCGCGTATCCATAATGATGGGAC                             <  SRR19973067.1367996/150‑1 (MQ=60)
                                                                                                                           GATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGC                           >  SRR19973067.1722658/1‑150 (MQ=60)
                                                                                                                           GATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGC                           >  SRR19973067.1722657/1‑150 (MQ=60)
                                                                                                                            ATAAACAGCGCGTCGAAATTGCCCTTTTCTAGTGTGAGTTAGCCCTGCAGCATTTGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAATGGGGCGGCGGCAGATAAAAACCGCGCCCGCGTATCCATAATGATGGGACGCG                          <  SRR19973067.511602/150‑1 (MQ=60)
                                                                                                                                ACAGCGCGTCTAAATTGCCTTTTTCTACTGTGTGTTAGCCCTGCAGCTTATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAATGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTT                      <  SRR19973067.3412855/150‑1 (MQ=60)
                                                                                                                                 CCGCGCGTCGAAATTGCCGTTTTCTAGTGTTTGTTAGCCCTGCAGCATTTGGCCCGCGACTATATCGATCGTGCCATGACGTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACCGCGCCCGCGTATCCATAATGATGGGACGCGTGTTT                     <  SRR19973067.1050206/150‑1 (MQ=60)
                                                                                                                                  CGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTA                    <  SRR19973067.2027889/150‑1 (MQ=60)
                                                                                                                                  AGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGATAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTA                    <  SRR19973067.2027802/150‑1 (MQ=60)
                                                                                                                                   GCGGGTCGAAATTGCCATTTACTACTGTTTTTTAGCCCTGCAGCATATGGCCAGCGACGATCTCTATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCATATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTAT                   <  SRR19973067.468208/150‑1 (MQ=60)
                                                                                                                                   GCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTAT                   <  SRR19973067.1487769/150‑1 (MQ=60)
                                                                                                                                   GCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCTTGACCTTGATAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTAT                   <  SRR19973067.1468512/150‑1 (MQ=60)
                                                                                                                                     GCGTCTAATTTGCCTTTTTCTACTGTGTGTTAGCCCTGCAGCATCTGGCCAGCGACGATCTCGATCGTGCCTTGACCTTGCTAAAAAAAGGGGCGGCGGCATATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGG                 <  SRR19973067.1271094/150‑1 (MQ=60)
                                                                                                                                       GTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCG               >  SRR19973067.2463229/1‑150 (MQ=60)
                                                                                                                                       GTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATATCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCG               >  SRR19973067.871196/1‑150 (MQ=60)
                                                                                                                                             TTTGCCCATTTTTAGTGTGAGTTAGCCCGGCAGCATATGGCCAGCTACGATCGCGAGGGTGCCAGGACCTTGCTAAAAAATGGGGGGGGGGCACATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGA         <  SRR19973067.117496/150‑1 (MQ=60)
                                                                                                                                             ATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAGTGATGGGACGCGTGTTTATGGCGAAAGGA         >  SRR19973067.402061/1‑150 (MQ=60)
                                                                                                                                             ATTGCCCATTTCTAGTGTGAGTTACCCCTGCAGCATAGGCCCAGCGACGATCTCGAGCGTGCCATGACCTTGAAAAAAAATGGGGCGGCGGCACATAAAAACCGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGA         <  SRR19973067.115177/150‑1 (MQ=60)
                                                                                                                                                GCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTTTTTATGGCGAAAGGAGAA      >  SRR19973067.15610/1‑150 (MQ=60)
                                                                                                                                                 CCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAAT     >  SRR19973067.456713/1‑150 (MQ=60)
                                                                                                                                                    TTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGGGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAATACG  <  SRR19973067.253456/150‑1 (MQ=60)
                                                                                                                                                    TTTTCTACTGTGAGTTAGCCCTGCAGCATAGGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAATACG  <  SRR19973067.252414/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAATACG  >  CP009273/1335208‑1335505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑