Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F534 I1 R1	1235	230.2	7611948	95.1%	7238962	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	1,335,356	A→T	H181L (CAT→CTT)	yciM →	envelope integrity maintenance protein; EnvC‑interacting protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,335,356	0	A	T	100.0%	693.3 / NA	207	H181L (CAT→CTT)	yciM	envelope integrity maintenance protein; EnvC‑interacting protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (108/99); total (108/99)

Only 100 of 210 total aligned reads displayed.

AGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAAT  >  CP009273/1335209‑1335502

aGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCttt                                                                                                                                                   >  2:1683157/1‑150 (MQ=255)

   tGACAGATGAAAATGACATCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGAAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTGTTCt                                                                                                                                                >  2:2842773/1‑150 (MQ=255)

    gACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTa                                                                                                                                               <  1:2210058/150‑1 (MQ=255)

      ccGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACt                                                                                                                                             >  2:152879/1‑150 (MQ=255)

      ccGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACt                                                                                                                                             >  2:2062520/1‑150 (MQ=255)

       cGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACtg                                                                                                                                            >  1:1102570/1‑150 (MQ=255)

       cGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACtg                                                                                                                                            >  2:3741496/1‑150 (MQ=255)

          tGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGa                                                                                                                                         <  1:914901/150‑1 (MQ=255)

          tGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGa                                                                                                                                         <  1:3358467/150‑1 (MQ=255)

           gAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAg                                                                                                                                        >  1:3675804/1‑150 (MQ=255)

              aCTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTa                                                                                                                                     <  1:521437/150‑1 (MQ=255)

                tGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGc                                                                                                                                   <  1:3185972/150‑1 (MQ=255)

                 gACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGcc                                                                                                                                  >  1:387321/1‑150 (MQ=255)

                        gCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTgcagc                                                                                                                           >  1:2843210/1‑150 (MQ=255)

                               cgcgcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGc                                                                                                                    <  1:3302023/150‑1 (MQ=255)

                                  gcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAg                                                                                                                 >  1:3571318/1‑150 (MQ=255)

                                  gcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAg                                                                                                                 >  1:2671771/1‑150 (MQ=255)

                                      cAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGcgac                                                                                                             <  2:3703935/150‑1 (MQ=255)

                                      cAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGcgac                                                                                                             <  1:2385978/150‑1 (MQ=255)

                                          aGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtc                                                                                                         >  2:1216333/1‑150 (MQ=255)

                                          aGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtc                                                                                                         >  1:25512/1‑150 (MQ=255)

                                           gTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtct                                                                                                        <  1:1226159/150‑1 (MQ=255)

                                           gTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtct                                                                                                        >  2:3300679/1‑150 (MQ=255)

                                            ttGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtctc                                                                                                       <  2:3460846/150‑1 (MQ=255)

                                                tACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATc                                                                                                   <  1:1236868/150‑1 (MQ=255)

                                                  cAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGt                                                                                                 >  2:158684/1‑150 (MQ=255)

                                                  cAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGt                                                                                                 >  2:1710926/1‑150 (MQ=255)

                                                   aaaTCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTg                                                                                                >  2:1443444/1‑150 (MQ=255)

                                                     aTCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGcc                                                                                              <  1:3549779/150‑1 (MQ=255)

                                                     aTCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGcc                                                                                              <  2:3264581/150‑1 (MQ=255)

                                                          ccAGGCTACTAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAc                                                                                         <  1:1912952/150‑1 (MQ=255)

                                                          ccAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAc                                                                                         <  1:1838091/150‑1 (MQ=255)

                                                           cAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAcc                                                                                        <  2:677772/150‑1 (MQ=255)

                                                            aGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCt                                                                                       >  1:1358425/1‑150 (MQ=255)

                                                             ggCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCtt                                                                                      <  2:2889434/150‑1 (MQ=255)

                                                                  ccAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑aa                                                                                >  2:2440455/1‑150 (MQ=255)

                                                                    agtgagtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑aaaa                                                                              >  1:760540/1‑150 (MQ=255)

                                                                       gagtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑aaaaaaa                                                                           >  2:297663/1‑150 (MQ=255)

                                                                        agtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAg                                                                          >  2:2571517/1‑150 (MQ=255)

                                                                              ataaaGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGggcgg                                                                    >  1:2485252/3‑150 (MQ=255)

                                                                               gAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGggcggc                                                                   <  1:568990/150‑1 (MQ=255)

                                                                                aaaGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGggcggcg                                                                  >  1:1989476/1‑150 (MQ=255)

                                                                                   gCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCa                                                               <  1:1471547/150‑1 (MQ=255)

                                                                                    cAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAg                                                              <  1:1041345/150‑1 (MQ=255)

                                                                                    cAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAg                                                              <  1:633446/150‑1 (MQ=255)

                                                                                    cAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAg                                                              <  1:633538/150‑1 (MQ=255)

                                                                                     aaTTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGa                                                             >  1:3163678/1‑150 (MQ=255)

                                                                                     aaTTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGa                                                             >  2:2259532/1‑150 (MQ=255)

                                                                                      aTTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGAt                                                            >  2:3046663/1‑150 (MQ=255)

                                                                                      aTTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGAt                                                            >  2:871129/1‑150 (MQ=255)

                                                                                        tGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATaa                                                          >  2:80872/1‑150 (MQ=255)

                                                                                          aTGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATaaaa                                                        >  2:3403406/1‑150 (MQ=255)

                                                                                          aTGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATaaaa                                                        >  2:2702103/1‑150 (MQ=255)

                                                                                              tGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAg                                                    >  2:3614646/1‑150 (MQ=255)

                                                                                              tGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAg                                                    >  2:1719972/1‑150 (MQ=255)

                                                                                               gCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAgc                                                   <  1:1340419/150‑1 (MQ=255)

                                                                                                 cGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAgcgc                                                 <  1:2247154/150‑1 (MQ=255)

                                                                                                  gAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑‑AAAAAAGGGGCGGCGGCAGATAAAAACAGCGccc                                               <  1:2360243/150‑1 (MQ=255)

                                                                                                   aaCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGccc                                               >  2:2620431/1‑150 (MQ=255)

                                                                                                   aaCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGccc                                               >  2:532975/1‑150 (MQ=255)

                                                                                                   aaCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGccc                                               >  2:218849/1‑150 (MQ=255)

                                                                                                    aCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCcg                                              <  2:1493527/150‑1 (MQ=255)

                                                                                                    aCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCcg                                              >  1:2873201/1‑150 (MQ=255)

                                                                                                        ctagTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTa                                          >  2:2184234/4‑150 (MQ=255)

                                                                                                         tGGTTAAGTTGGGTAAATATAAACAGCGTGTCGAAATTGCCTTTTTCTACTGTGTGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGAAGATAAAAACAGCGCCCGCGTAt                                         <  1:640578/150‑1 (MQ=255)

                                                                                                         tGGTGAAGTTGGGTAAAGATAAATAGCGCGTTGAAATTGCCCTTTTTTACTGTGAGTTAGCCCTGCAGCATATGGCAAGCGACGATATCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCATATAAAAACAGCGCCCGCGTAt                                         <  1:3232797/150‑1 (MQ=255)

                                                                                                         tGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAt                                         >  1:3433107/1‑150 (MQ=255)

                                                                                                         tGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATAGGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAt                                         <  1:1683157/150‑1 (MQ=255)

                                                                                                           gTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATcc                                       >  2:3202613/1‑150 (MQ=255)

                                                                                                            tGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCa                                      >  2:1967309/1‑150 (MQ=255)

                                                                                                             gAAGCTGGGTAAATATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCAt                                     <  1:1419783/150‑1 (MQ=255)

                                                                                                                 cTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatg                                 >  1:1638518/1‑150 (MQ=255)

                                                                                                                  tGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTCTCCATAatga                                >  1:2745413/1‑150 (MQ=255)

                                                                                                                  tGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatga                                >  1:869355/1‑150 (MQ=255)

                                                                                                                  tGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatga                                >  1:137066/1‑150 (MQ=255)

                                                                                                                   gggTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatgat                               <  1:1735052/150‑1 (MQ=255)

                                                                                                                     gTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATgg                             <  2:2340721/150‑1 (MQ=255)

                                                                                                                      tAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATggg                            <  2:3675804/150‑1 (MQ=255)

                                                                                                                      tAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATggg                            >  2:3397670/1‑150 (MQ=255)

                                                                                                                        aaGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGAc                          <  1:3639814/150‑1 (MQ=255)

                                                                                                                             aaaCAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCgtg                     >  2:1887127/1‑150 (MQ=255)

                                                                                                                                 aGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTa                 <  2:25512/150‑1 (MQ=255)

                                                                                                                                 aGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTa                 >  1:1670566/1‑150 (MQ=255)

                                                                                                                                    gcgTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATgg              <  2:639044/150‑1 (MQ=255)

                                                                                                                                    gcgTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATgg              <  1:2281576/150‑1 (MQ=255)

                                                                                                                                     cgTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGc             >  2:421575/1‑150 (MQ=255)

                                                                                                                                       tCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATTAAAACAGCGCCCGCGTATCCATAATTATTGGACGAGTGTTTATGGCGa           <  2:2677411/150‑1 (MQ=255)

                                                                                                                                        cGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGa           >  1:1095938/1‑150 (MQ=255)

                                                                                                                                         gAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGaaa         >  1:578605/1‑150 (MQ=255)

                                                                                                                                         gAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGaaa         >  2:1299672/1‑150 (MQ=255)

                                                                                                                                          aaaTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAg        <  1:80402/150‑1 (MQ=255)

                                                                                                                                          aaaTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAg        >  1:1696724/1‑150 (MQ=255)

                                                                                                                                           aaTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAgg       <  2:2368532/150‑1 (MQ=255)

                                                                                                                                            aTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGga      <  1:152879/150‑1 (MQ=255)

                                                                                                                                            aTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGga      <  1:2062520/150‑1 (MQ=255)

                                                                                                                                             ttGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGgag     <  1:1875437/150‑1 (MQ=255)

                                                                                                                                                cccTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGTCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAAt  >  2:1403786/1‑150 (MQ=255)

                                                                                                                                                cccTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATGATGATGGGACGCGTGTTTATGGCGAAAGGAGAAt  <  2:3163678/150‑1 (MQ=255)

                                                                                                                                                cccTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAAt  <  1:3123791/150‑1 (MQ=255)

                                                                                                                                                cccTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAAt  <  2:1394431/150‑1 (MQ=255)

AGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAAT  >  CP009273/1335209‑1335502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAATA  >  CP009273/1335213‑1335503

GACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTA                                                                                                                                                <  SRR19973061.2215607/150‑1 (MQ=60)

   CGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTG                                                                                                                                             >  SRR19973061.1105388/1‑150 (MQ=60)

      TGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGA                                                                                                                                          <  SRR19973061.917243/150‑1 (MQ=60)

      TGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGA                                                                                                                                          <  SRR19973061.3367104/150‑1 (MQ=60)

       GAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAG                                                                                                                                         >  SRR19973061.3685199/1‑150 (MQ=60)

         AACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTT                                                                                                                                       >  SRR19973061.640669/1‑150 (MQ=60)

          ACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTA                                                                                                                                      <  SRR19973061.522813/150‑1 (MQ=60)

            TGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGC                                                                                                                                    <  SRR19973061.3194146/150‑1 (MQ=60)

             GACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCC                                                                                                                                   >  SRR19973061.388378/1‑150 (MQ=60)

               CTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCT                                                                                                                                 <  SRR19973061.573764/150‑1 (MQ=60)

                TTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTG                                                                                                                                >  SRR19973061.1397957/1‑150 (MQ=60)

                    GCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGC                                                                                                                            >  SRR19973061.2850496/1‑150 (MQ=60)

                      ATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCAT                                                                                                                          >  SRR19973061.2374562/1‑150 (MQ=60)

                           CGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGC                                                                                                                     <  SRR19973061.3310522/150‑1 (MQ=60)

                           CGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGC                                                                                                                     >  SRR19973061.1723478/1‑150 (MQ=60)

                              GCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAG                                                                                                                  >  SRR19973061.3580469/1‑150 (MQ=60)

                              GCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAG                                                                                                                  >  SRR19973061.2678645/1‑150 (MQ=60)

                               CTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGTTGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGC                                                                                                                 <  SRR19973061.1504504/150‑1 (MQ=60)

                                  CAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGAC                                                                                                              <  SRR19973061.2392062/150‑1 (MQ=60)

                                   AACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACG                                                                                                             >  SRR19973061.2857421/1‑150 (MQ=60)

                                   AACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACG                                                                                                             <  SRR19973061.2267471/150‑1 (MQ=60)

                                      AGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATC                                                                                                          >  SRR19973061.25608/1‑150 (MQ=60)

                                      AGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATC                                                                                                          >  SRR19973061.1443699/1‑150 (MQ=60)

                                       GTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCT                                                                                                         <  SRR19973061.1206424/150‑1 (MQ=60)

                                       GTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCT                                                                                                         <  SRR19973061.1229216/150‑1 (MQ=60)

                                            TACAAATCTTCCAGGCTAACAGTGTGTGGCAGAAAGCAATTGTTGTTGCCGTACGCCTGGTGAATCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATC                                                                                                    <  SRR19973061.1239881/150‑1 (MQ=60)

                                            TACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATC                                                                                                    <  SRR19973061.3146577/150‑1 (MQ=60)

                                            TACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATC                                                                                                    <  SRR19973061.1239940/150‑1 (MQ=60)

                                                 ATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCC                                                                                               <  SRR19973061.3558866/150‑1 (MQ=60)

                                                 ATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCC                                                                                               <  SRR19973061.3559525/150‑1 (MQ=60)

                                                  TCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCA                                                                                              <  SRR19973061.559179/150‑1 (MQ=60)

                                                    TACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATG                                                                                            <  SRR19973061.1897615/150‑1 (MQ=60)

                                                    TACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATG                                                                                            >  SRR19973061.3577558/1‑150 (MQ=60)

                                                      CCAGGCTACTAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAC                                                                                          <  SRR19973061.1917682/150‑1 (MQ=60)

                                                      CCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAC                                                                                          <  SRR19973061.1842693/150‑1 (MQ=60)

                                                      CCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAC                                                                                          <  SRR19973061.1842697/150‑1 (MQ=60)

                                                        AGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCT                                                                                        >  SRR19973061.1361848/1‑150 (MQ=60)

                                                        AGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCT                                                                                        >  SRR19973061.2069095/1‑150 (MQ=60)

                                                             ACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑A                                                                                  <  SRR19973061.3026991/150‑1 (MQ=60)

                                                               CAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAA                                                                                <  SRR19973061.3183025/150‑1 (MQ=60)

                                                               CAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAA                                                                                <  SRR19973061.2514612/150‑1 (MQ=60)

                                                                AGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAA                                                                               >  SRR19973061.2335258/1‑150 (MQ=60)

                                                                AGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAA                                                                               >  SRR19973061.762534/1‑150 (MQ=60)

                                                                 GTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAA                                                                              <  SRR19973061.1363840/150‑1 (MQ=60)

                                                                 GTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAA                                                                              >  SRR19973061.3494942/1‑150 (MQ=60)

                                                                       GGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGG                                                                        <  SRR19973061.1759920/150‑1 (MQ=60)

                                                                          ATAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGG                                                                     >  SRR19973061.2491577/1‑150 (MQ=60)

                                                                           GAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGC                                                                    <  SRR19973061.570476/150‑1 (MQ=60)

                                                                            AAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCG                                                                   >  SRR19973061.1994458/1‑150 (MQ=60)

                                                                            AAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCG                                                                   >  SRR19973061.1994353/1‑150 (MQ=60)

                                                                               GCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCA                                                                <  SRR19973061.1475244/150‑1 (MQ=60)

                                                                               GCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCA                                                                <  SRR19973061.1475245/150‑1 (MQ=60)

                                                                                CAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAG                                                               <  SRR19973061.1044011/150‑1 (MQ=60)

                                                                                CAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAG                                                               <  SRR19973061.635151/150‑1 (MQ=60)

                                                                                CAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAG                                                               <  SRR19973061.635147/150‑1 (MQ=60)

                                                                                CAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAG                                                               <  SRR19973061.635059/150‑1 (MQ=60)

                                                                                 AATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGA                                                              >  SRR19973061.2032904/1‑150 (MQ=60)

                                                                                 AATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGA                                                              >  SRR19973061.3171800/1‑150 (MQ=60)

                                                                                           GCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGC                                                    <  SRR19973061.1099320/150‑1 (MQ=60)

                                                                                           GCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGC                                                    <  SRR19973061.1343789/150‑1 (MQ=60)

                                                                                            CCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCG                                                   >  SRR19973061.1622730/1‑150 (MQ=60)

                                                                                             CGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGTCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGC                                                  >  SRR19973061.105214/1‑150 (MQ=60)

                                                                                             CGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGC                                                  <  SRR19973061.2252804/150‑1 (MQ=60)

                                                                                              GAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCC                                                 >  SRR19973061.1353001/1‑150 (MQ=60)

                                                                                              GAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCC                                                 >  SRR19973061.3288348/1‑150 (MQ=60)

                                                                                              GAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑‑AAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCC                                                <  SRR19973061.2366242/150‑1 (MQ=60)

                                                                                              GAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑‑AAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCC                                                <  SRR19973061.453845/150‑1 (MQ=60)

                                                                                               AACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCC                                                >  SRR19973061.2839705/1‑150 (MQ=60)

                                                                                                ACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCG                                               >  SRR19973061.2880567/1‑150 (MQ=60)

                                                                                                 CGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGC                                              >  SRR19973061.597679/1‑150 (MQ=60)

                                                                                                 CGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATATCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGC                                              <  SRR19973061.3583069/150‑1 (MQ=60)

                                                                                                  GCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCG                                             >  SRR19973061.2653179/1‑150 (MQ=60)

                                                                                                     TGGTTAAGTTGGGTAAATATAAACAGCGTGTCGAAATTGCCTTTTTCTACTGTGTGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGAAGATAAAAACAGCGCCCGCGTAT                                          <  SRR19973061.642211/150‑1 (MQ=60)

                                                                                                     TGGTGAAGTTGGGTAAAGATAAATAGCGCGTTGAAATTGCCCTTTTTTACTGTGAGTTAGCCCTGCAGCATATGGCAAGCGACGATATCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCATATAAAAACAGCGCCCGCGTAT                                          <  SRR19973061.3241105/150‑1 (MQ=60)

                                                                                                     TGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAT                                          <  SRR19973061.1687365/150‑1 (MQ=60)

                                                                                                     TGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAT                                          >  SRR19973061.3441912/1‑150 (MQ=60)

                                                                                                     TGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAT                                          >  SRR19973061.3694125/1‑150 (MQ=60)

                                                                                                     TGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATAGGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAT                                          <  SRR19973061.1687386/150‑1 (MQ=60)

                                                                                                        TGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCA                                       >  SRR19973061.1328031/1‑150 (MQ=60)

                                                                                                         GAAGCTGGGTAAATATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCAT                                      <  SRR19973061.1423363/150‑1 (MQ=60)

                                                                                                             CTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATG                                  >  SRR19973061.1642625/1‑150 (MQ=60)

                                                                                                             CTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATG                                  >  SRR19973061.1642639/1‑150 (MQ=60)

                                                                                                              TGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTCTCCATAATGA                                 >  SRR19973061.2752477/1‑150 (MQ=60)

                                                                                                              TGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGA                                 >  SRR19973061.871613/1‑150 (MQ=60)

                                                                                                              TGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGA                                 >  SRR19973061.137505/1‑150 (MQ=60)

                                                                                                               GGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGAT                                <  SRR19973061.1739412/150‑1 (MQ=60)

                                                                                                                   AAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATagatcgg                            >  SRR19973061.335170/1‑143 (MQ=60)

                                                                                                                   AAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGA                            <  SRR19973061.2443962/150‑1 (MQ=60)

                                                                                                                   AAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGA                            >  SRR19973061.81417/1‑150 (MQ=60)

                                                                                                                    AAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGAC                           <  SRR19973061.3649116/150‑1 (MQ=60)

                                                                                                                    AAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGAC                           <  SRR19973061.3649177/150‑1 (MQ=60)

                                                                                                                    AAGATAAACAGCGAGTCGAAATTGCCATTTTATATTGTGAGTTAGCCCTGCAGAATATGGCAAGAGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGAAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGAC                           <  SRR19973061.2774957/150‑1 (MQ=60)

                                                                                                                          AACAGCGCGTCGAAATTGCCCTTTTATACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGT                     <  SRR19973061.343583/150‑1 (MQ=60)

                                                                                                                           ACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTT                    <  SRR19973061.801655/150‑1 (MQ=60)

                                                                                                                            CAGCGAGTCGAAAGTGCCTTTTTCTTCTGTGAGTTAGCCCTGTAGCATATGGCCAGCGACGATTTCGATCGTGCAATGACCTTGTT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTT                   <  SRR19973061.2005210/150‑1 (MQ=60)

                                                                                                                             AGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTA                  >  SRR19973061.1674758/1‑150 (MQ=60)

                                                                                                                                GCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGG               <  SRR19973061.2693376/150‑1 (MQ=60)

                                                                                                                                GCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGG               <  SRR19973061.908046/150‑1 (MQ=60)

                                                                                                                                GCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGG               <  SRR19973061.2287340/150‑1 (MQ=60)

                                                                                                                                    CGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGA            >  SRR19973061.1098746/1‑150 (MQ=60)

                                                                                                                                    CGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAA           >  SRR19973061.2220604/1‑150 (MQ=60)

                                                                                                                                     GAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAA          >  SRR19973061.580114/1‑150 (MQ=60)

                                                                                                                                      AAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAG         <  SRR19973061.80689/150‑1 (MQ=60)

                                                                                                                                      AAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAG         >  SRR19973061.1700984/1‑150 (MQ=60)

                                                                                                                                      AAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAG         >  SRR19973061.1700892/1‑150 (MQ=60)

                                                                                                                                       AATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGG        >  SRR19973061.689701/1‑150 (MQ=60)

                                                                                                                                       AATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGG        >  SRR19973061.689818/1‑150 (MQ=60)

                                                                                                                                        ATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGA       <  SRR19973061.153359/150‑1 (MQ=60)

                                                                                                                                        ATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGA       <  SRR19973061.2067712/150‑1 (MQ=60)

                                                                                                                                         TTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAG      <  SRR19973061.1880106/150‑1 (MQ=60)

                                                                                                                                           GCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAA    >  SRR19973061.3197382/1‑150 (MQ=60)

                                                                                                                                            CCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAAT   <  SRR19973061.3131842/150‑1 (MQ=60)

                                                                                                                                             CCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAATA  >  SRR19973061.704559/1‑150 (MQ=60)

GACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT‑AAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAATA  >  CP009273/1335213‑1335503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`