Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,335,356 A→T 100% H181L (CAT→CTT)  yciM → envelope integrity maintenance protein; EnvC‑interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,335,3560AT100.0% 490.9 / NA 150H181L (CAT→CTT) yciMenvelope integrity maintenance protein; EnvC‑interacting protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (85/65);  total (85/65)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 157 total aligned reads displayed.

CTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGA  >  CP009273/1335211‑1335500
                                                                                                                                                 |                                                                                                                                                
cTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCttt                                                                                                                                                <  2:183230/148‑1 (MQ=255)
cTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCttt                                                                                                                                                >  2:916645/1‑148 (MQ=255)
 tGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCtttt                                                                                                                                               >  1:1033348/1‑148 (MQ=255)
 tGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCtttt                                                                                                                                               >  1:1033310/1‑148 (MQ=255)
 tGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCtttt                                                                                                                                               <  2:371222/148‑1 (MQ=255)
 tGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCtttt                                                                                                                                               <  2:405717/148‑1 (MQ=255)
 tGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCtttt                                                                                                                                               <  1:260643/148‑1 (MQ=255)
  gACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTc                                                                                                                                              >  2:464732/1‑148 (MQ=255)
  gACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCGGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTc                                                                                                                                              >  2:464738/1‑148 (MQ=255)
      gATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACt                                                                                                                                          <  2:266056/148‑1 (MQ=255)
      gATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACt                                                                                                                                          <  2:1057366/148‑1 (MQ=255)
       aTGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACtg                                                                                                                                         <  1:551732/148‑1 (MQ=255)
        tGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACtgt                                                                                                                                        >  2:2607307/1‑148 (MQ=255)
            aCTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGt                                                                                                                                    >  1:2932389/1‑148 (MQ=255)
              tGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTa                                                                                                                                  <  2:2886240/148‑1 (MQ=255)
              tGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTa                                                                                                                                  <  2:2886247/148‑1 (MQ=255)
               gACGTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAg                                                                                                                                 <  2:2399626/148‑1 (MQ=255)
                aCTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGc                                                                                                                                >  1:2228674/1‑148 (MQ=255)
                 cTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGcc                                                                                                                               <  1:2073078/148‑1 (MQ=255)
                  ttCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGccc                                                                                                                              >  2:1997138/1‑148 (MQ=255)
                       cATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTgcag                                                                                                                         >  2:1842252/1‑148 (MQ=255)
                       cATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTgcag                                                                                                                         >  1:1336754/1‑148 (MQ=255)
                       cATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTgcag                                                                                                                         >  1:1696729/1‑148 (MQ=255)
                        aTTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTgcagc                                                                                                                        >  1:1397058/1‑148 (MQ=255)
                         ttGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTgcagca                                                                                                                       <  2:771761/148‑1 (MQ=255)
                          tGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCat                                                                                                                      <  2:3045761/148‑1 (MQ=255)
                          tGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCat                                                                                                                      <  2:733066/148‑1 (MQ=255)
                               cgcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGc                                                                                                                 >  1:1840220/1‑148 (MQ=255)
                               cgcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGc                                                                                                                 <  2:3100227/148‑1 (MQ=255)
                                          ttGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtc                                                                                                      <  2:275704/148‑1 (MQ=255)
                                          ttGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtc                                                                                                      >  1:882567/1‑148 (MQ=255)
                                           tGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGAtct                                                                                                     <  2:1661864/148‑1 (MQ=255)
                                              tACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGa                                                                                                  <  1:3331476/148‑1 (MQ=255)
                                                   aTCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTg                                                                                             <  2:302613/148‑1 (MQ=255)
                                                   aTCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTg                                                                                             >  1:3012935/1‑148 (MQ=255)
                                                   aTCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTg                                                                                             <  2:1650616/148‑1 (MQ=255)
                                                       aCCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCAt                                                                                         >  2:1305722/1‑148 (MQ=255)
                                                        ccAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATg                                                                                        <  1:945696/148‑1 (MQ=255)
                                                         cAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGa                                                                                       <  1:155877/148‑1 (MQ=255)
                                                         cAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGa                                                                                       <  1:1930293/148‑1 (MQ=255)
                                                           ggCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAcc                                                                                     <  2:938030/148‑1 (MQ=255)
                                                            gCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCt                                                                                    >  1:1933908/1‑148 (MQ=255)
                                                            gCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCt                                                                                    <  1:1228847/148‑1 (MQ=255)
                                                              tACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTg                                                                                  <  2:2536634/148‑1 (MQ=255)
                                                               aCCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGc                                                                                 >  1:2915185/1‑148 (MQ=255)
                                                                  agtgagtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTaa                                                                              >  2:212721/1‑148 (MQ=255)
                                                                      agtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTaaaaaa                                                                          >  2:1203230/1‑148 (MQ=255)
                                                                       gtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTaaaaaaa                                                                         <  2:2206355/148‑1 (MQ=255)
                                                                       gtgGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTaaaaaaa                                                                         <  2:1393037/148‑1 (MQ=255)
                                                                             gAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGggcg                                                                   <  2:1332221/148‑1 (MQ=255)
                                                                              aaaGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGggcgg                                                                  <  1:1653975/148‑1 (MQ=255)
                                                                              aaaGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGggcgg                                                                  >  1:3033787/1‑148 (MQ=255)
                                                                                     ttGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGa                                                           >  1:2136365/1‑148 (MQ=255)
                                                                                     ttGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGa                                                           <  1:2385510/148‑1 (MQ=255)
                                                                                     ttGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGa                                                           >  1:2487838/1‑148 (MQ=255)
                                                                                     ttGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGa                                                           >  1:1417729/1‑148 (MQ=255)
                                                                                         tggtGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACCGCGCGTCGAAATTGCCCTATTCTACTGTGAGTTAGCCCGGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATaaa                                                       <  2:3245205/145‑1 (MQ=255)
                                                                                         tGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATaaa                                                       <  2:3245007/148‑1 (MQ=255)
                                                                                          gTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATaaaa                                                      <  2:2897111/148‑1 (MQ=255)
                                                                                            tGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAAc                                                    <  1:1359345/148‑1 (MQ=255)
                                                                                            tGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAAc                                                    <  1:588695/148‑1 (MQ=255)
                                                                                             gCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACa                                                   <  1:1263297/148‑1 (MQ=255)
                                                                                                  aCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGcc                                              >  1:1856721/1‑148 (MQ=255)
                                                                                                  aCGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGcc                                              >  1:568761/1‑148 (MQ=255)
                                                                                                   cGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGccc                                             >  2:228830/1‑148 (MQ=255)
                                                                                                   cGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGccc                                             >  2:1096826/1‑148 (MQ=255)
                                                                                                       tGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGt                                         >  1:2715773/1‑148 (MQ=255)
                                                                                                         gTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAt                                       >  1:3306172/1‑148 (MQ=255)
                                                                                                           gAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATcc                                     >  2:1223139/1‑148 (MQ=255)
                                                                                                             aGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCAt                                   >  2:610667/1‑148 (MQ=255)
                                                                                                               cTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATaa                                 >  2:2827545/1‑148 (MQ=255)
                                                                                                               cTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATaa                                 >  2:2827551/1‑148 (MQ=255)
                                                                                                               cTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATaa                                 >  1:2032793/1‑148 (MQ=255)
                                                                                                                  ggTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatga                              >  1:2542161/1‑148 (MQ=255)
                                                                                                                  ggTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatga                              >  1:466471/1‑148 (MQ=255)
                                                                                                                  ggTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatga                              >  1:2542160/1‑148 (MQ=255)
                                                                                                                   gTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatgat                             <  2:1140001/148‑1 (MQ=255)
                                                                                                                   gTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatgat                             <  2:1139793/148‑1 (MQ=255)
                                                                                                                    tAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatgatg                            >  2:2807464/1‑148 (MQ=255)
                                                                                                                    tAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAACTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAatgatg                            >  1:2227655/1‑148 (MQ=255)
                                                                                                                     aaaGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATgg                           >  2:990012/1‑148 (MQ=255)
                                                                                                                          tAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGAcgc                      <  2:2815524/148‑1 (MQ=255)
                                                                                                                           aaaCAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGAcgcg                     <  2:882567/148‑1 (MQ=255)
                                                                                                                           aaaCAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGAcgcg                     >  1:480646/1‑148 (MQ=255)
                                                                                                                             accgcgcGTCGAATTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCgtg                   <  2:1488538/145‑1 (MQ=255)
                                                                                                                             aCAGCGGGTCTAATTTGCCCTTTTCTACTGTGATTTCGCCCTGCAGCATAGGCCCAGCGACGATCTCGATCGTGCCATGACCTTGATAAAAAATGGGGGGGCGGAAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCgtg                   <  1:79312/148‑1 (MQ=255)
                                                                                                                              cAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCgtgt                  >  2:1164298/1‑148 (MQ=255)
                                                                                                                                gcgcGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGttt                <  2:2487838/148‑1 (MQ=255)
                                                                                                                                   cggcGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATCTGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATg             <  2:792917/145‑1 (MQ=255)
                                                                                                                                     tCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGc           >  1:2941719/1‑148 (MQ=255)
                                                                                                                                      cGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCg          >  2:2717881/1‑148 (MQ=255)
                                                                                                                                       gAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGa         <  2:574853/148‑1 (MQ=255)
                                                                                                                                       gAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGa         <  2:2339437/148‑1 (MQ=255)
                                                                                                                                        aaaTTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGaa        >  1:755835/1‑148 (MQ=255)
                                                                                                                                        aaaTGGCCCTTTTCTCCTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGaa        <  2:85012/148‑1 (MQ=255)
                                                                                                                                           ttGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAgg     >  1:2535065/1‑148 (MQ=255)
                                                                                                                                           ttGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAgg     >  1:774424/1‑148 (MQ=255)
                                                                                                                                              cccTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGgaga  >  2:2931152/1‑148 (MQ=255)
                                                                                                                                              cccTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGgaga  >  2:2384255/1‑148 (MQ=255)
                                                                                                                                              cccTTTTCTACTGTGAGTTAGCCCTGCAGCATAGGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGgaga  <  2:3475854/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGA  >  CP009273/1335211‑1335500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAATAC  >  CP009273/1335210‑1335504
                                                                                                                                                  |                                                                                                                                                    
GCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTT                                                                                                                                                   >  SRR19973065.1040189/1‑150 (MQ=60)
GCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTT                                                                                                                                                   >  SRR19973065.1040150/1‑150 (MQ=60)
 CTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTC                                                                                                                                                  >  SRR19973065.944231/1‑150 (MQ=60)
  TGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCT                                                                                                                                                 <  SRR19973065.262442/150‑1 (MQ=60)
        ATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTG                                                                                                                                           <  SRR19973065.555275/150‑1 (MQ=60)
         TGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGA                                                                                                                                          >  SRR19973065.96116/1‑150 (MQ=60)
         TGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGA                                                                                                                                          >  SRR19973065.1885012/1‑150 (MQ=60)
           AAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGT                                                                                                                                        >  SRR19973065.2950179/1‑150 (MQ=60)
              CTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAG                                                                                                                                     <  SRR19973065.2520301/150‑1 (MQ=60)
               TGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGC                                                                                                                                    >  SRR19973065.2242865/1‑150 (MQ=60)
                  CTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCT                                                                                                                                 <  SRR19973065.2086308/150‑1 (MQ=60)
                      CGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAG                                                                                                                             >  SRR19973065.1345608/1‑150 (MQ=60)
                      CGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAG                                                                                                                             >  SRR19973065.1708051/1‑150 (MQ=60)
                      CGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAG                                                                                                                             >  SRR19973065.3456707/1‑150 (MQ=60)
                       GCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGC                                                                                                                            >  SRR19973065.1406229/1‑150 (MQ=60)
                              CGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGC                                                                                                                     >  SRR19973065.1852163/1‑150 (MQ=60)
                              CGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGC                                                                                                                     >  SRR19973065.1852357/1‑150 (MQ=60)
                              CGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGC                                                                                                                     >  SRR19973065.533674/1‑150 (MQ=60)
                                         AGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATC                                                                                                          >  SRR19973065.888186/1‑150 (MQ=60)
                                           TTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTC                                                                                                        <  SRR19973065.1536419/150‑1 (MQ=60)
                                               TACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATC                                                                                                    <  SRR19973065.3351811/150‑1 (MQ=60)
                                                  AAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTG                                                                                                 >  SRR19973065.3031386/1‑150 (MQ=60)
                                                         CCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAC                                                                                          <  SRR19973065.951946/150‑1 (MQ=60)
                                                          CAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACC                                                                                         <  SRR19973065.1942936/150‑1 (MQ=60)
                                                          CAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACC                                                                                         <  SRR19973065.157170/150‑1 (MQ=60)
                                                           AGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCT                                                                                        >  SRR19973065.1946568/1‑150 (MQ=60)
                                                           AGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCT                                                                                        >  SRR19973065.157795/1‑150 (MQ=60)
                                                           AGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCT                                                                                        >  SRR19973065.566093/1‑150 (MQ=60)
                                                            GGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTT                                                                                       >  SRR19973065.756547/1‑150 (MQ=60)
                                                            GGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTT                                                                                       >  SRR19973065.2235386/1‑150 (MQ=60)
                                                             GCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTG                                                                                      <  SRR19973065.1332656/150‑1 (MQ=60)
                                                             GCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTG                                                                                      <  SRR19973065.1236872/150‑1 (MQ=60)
                                                              CTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGC                                                                                     >  SRR19973065.2932914/1‑150 (MQ=60)
                                                               TACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCT                                                                                    <  SRR19973065.1060758/150‑1 (MQ=60)
                                                                ACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTA                                                                                   <  SRR19973065.2801223/150‑1 (MQ=60)
                                                                             AGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGG                                                                      >  SRR19973065.3052369/1‑150 (MQ=60)
                                                                               AAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCG                                                                    <  SRR19973065.1665063/150‑1 (MQ=60)
                                                                               AAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCG                                                                    >  SRR19973065.138099/1‑150 (MQ=60)
                                                                                    AATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGA                                                               >  SRR19973065.2503491/1‑150 (MQ=60)
                                                                                    AATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGA                                                               >  SRR19973065.2150111/1‑150 (MQ=60)
                                                                                    AATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGA                                                               >  SRR19973065.1426985/1‑150 (MQ=60)
                                                                                      TTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATA                                                             <  SRR19973065.2400472/150‑1 (MQ=60)
                                                                                             TGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAG                                                      <  SRR19973065.592348/150‑1 (MQ=60)
                                                                                             TGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAG                                                      <  SRR19973065.3125216/150‑1 (MQ=60)
                                                                                             TGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAG                                                      <  SRR19973065.1368347/150‑1 (MQ=60)
                                                                                              GCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGC                                                     <  SRR19973065.1271620/150‑1 (MQ=60)
                                                                                                 GAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCC                                                  >  SRR19973065.2014421/1‑150 (MQ=60)
                                                                                                 GAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCC                                                  >  SRR19973065.572361/1‑150 (MQ=60)
                                                                                                 GAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCC                                                  >  SRR19973065.1868937/1‑150 (MQ=60)
                                                                                                      CCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGT                                             >  SRR19973065.2732666/1‑150 (MQ=60)
                                                                                                        TGGTGATGGTGGGTAAAGATAAACCGCGCGTAGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGAATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAT                                           <  SRR19973065.3244920/150‑1 (MQ=60)
                                                                                                        TGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTAT                                           >  SRR19973065.3326316/1‑150 (MQ=60)
                                                                                                         GGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATC                                          >  SRR19973065.2567740/1‑150 (MQ=60)
                                                                                                              AGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAA                                     >  SRR19973065.2045837/1‑150 (MQ=60)
                                                                                                              AGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAA                                     >  SRR19973065.2045843/1‑150 (MQ=60)
                                                                                                                 TGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGA                                  >  SRR19973065.469681/1‑150 (MQ=60)
                                                                                                                 TGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGA                                  >  SRR19973065.2558022/1‑150 (MQ=60)
                                                                                                                 TGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGA                                  >  SRR19973065.2558082/1‑150 (MQ=60)
                                                                                                                 TGGGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGA                                  >  SRR19973065.2558083/1‑150 (MQ=60)
                                                                                                                   GGTAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGAACTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATG                                >  SRR19973065.2241845/1‑150 (MQ=60)
                                                                                                                     TAAAGATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGG                              >  SRR19973065.2969804/1‑150 (MQ=60)
                                                                                                                       aagataccccGCGCGTCGAAATGGCCCTTTTCTCCTGTGAGTTCGCCCTGCAGCATAGGGCCCGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAACGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGAC                            <  SRR19973065.1848466/140‑1 (MQ=60)
                                                                                                                          ATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCG                         >  SRR19973065.483919/1‑150 (MQ=60)
                                                                                                                          ATAAACAGCGCGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCG                         >  SRR19973065.2260018/1‑150 (MQ=60)
                                                                                                                              cccGCGCGTCTAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCATATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTT                     <  SRR19973065.79991/147‑1 (MQ=60)
                                                                                                                              ACAGCGGGTCTAATTTGCCCTTTTCTACTGTGATTTCGCCCTGCAGCATAGGCCCAGCGACGATCTCGATCGTGCCATGACCTTGATAAAAAATGGGGGGGCGGAAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTT                     <  SRR19973065.80112/150‑1 (MQ=60)
                                                                                                                                    CGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGC               >  SRR19973065.2959546/1‑150 (MQ=60)
                                                                                                                                    CGTCGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGC               >  SRR19973065.2959578/1‑150 (MQ=60)
                                                                                                                                       CGAAATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAA            >  SRR19973065.760874/1‑150 (MQ=60)
                                                                                                                                          AATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGG         >  SRR19973065.779566/1‑150 (MQ=60)
                                                                                                                                          AATTGCCCTTTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGG         >  SRR19973065.2550968/1‑150 (MQ=60)
                                                                                                                                                 CTTTTCTACTGTGAGTTAGCCCTGCAGCATCTGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAATAC  <  SRR19973065.1111592/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
GCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTGGTGAAGCTGGGTAAAGATAAACAGCGCGTCGAAATTGCCCATTTCTACTGTGAGTTAGCCCTGCAGCATATGGCCAGCGACGATCTCGATCGTGCCATGACCTTGCTAAAAAAAGGGGCGGCGGCAGATAAAAACAGCGCCCGCGTATCCATAATGATGGGACGCGTGTTTATGGCGAAAGGAGAATAC  >  CP009273/1335210‑1335504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑