Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,751,135 G→T 23.1% S18* (TCA→TAA)  yiaY ← putative Fe‑containing alcohol dehydrogenase, Pfam00465 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,751,1350GT23.1% 2422.5 / inf 1013S18* (TCA→TAA) yiaYputative Fe‑containing alcohol dehydrogenase, Pfam00465 family
Reads supporting (aligned to +/- strand):  ref base G (437/342);  new base T (130/104);  total (567/446)
Fisher's exact test for biased strand distribution p-value = 8.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.52e-01

Only 100 of 1020 total aligned reads displayed.

CCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTA  >  CP009273/3750988‑3751282
                                                                                                                                                   |                                                                                                                                                   
  atcatAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGaa                                                                                                                                                   <  2:2988153/148‑1 (MQ=255)
  atcatAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGaa                                                                                                                                                   <  1:1354713/148‑1 (MQ=255)
   tcatAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAAt                                                                                                                                                  >  1:1231549/1‑148 (MQ=255)
   tcatAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAAt                                                                                                                                                  <  1:3528715/148‑1 (MQ=255)
   tcatAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAAt                                                                                                                                                  <  1:1280002/148‑1 (MQ=255)
    catAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATc                                                                                                                                                 >  1:2889627/1‑148 (MQ=255)
     ataaataaCGCTACAAAGAGTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATCTTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCa                                                                                                                                                <  2:2358974/148‑1 (MQ=255)
     ataaataaCGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCa                                                                                                                                                <  1:3289396/148‑1 (MQ=255)
        aataaCGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAgcg                                                                                                                                             >  1:402041/1‑148 (MQ=255)
        aataaCGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAgcg                                                                                                                                             >  2:2177860/1‑148 (MQ=255)
         ataaCGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAgcgc                                                                                                                                            >  2:157760/1‑148 (MQ=255)
            aCGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGa                                                                                                                                         >  1:1655863/1‑148 (MQ=255)
             cGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGAt                                                                                                                                        >  2:2416927/1‑148 (MQ=255)
             cGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGAt                                                                                                                                        >  1:2403067/1‑148 (MQ=255)
                 aaaaaTATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACa                                                                                                                                    >  1:2711884/1‑148 (MQ=255)
                 aaaaaTATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACa                                                                                                                                    >  2:333594/1‑148 (MQ=255)
                  aaaaTATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACAt                                                                                                                                   >  1:1926919/1‑148 (MQ=255)
                   aaaTATTGCGTTCTTCCAGTGCTTTTTGCACATCGCACGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACAtt                                                                                                                                  >  2:2052675/1‑148 (MQ=255)
                     atatTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGAATCAGTCAATGAATCAGCGCCGATGACATTca                                                                                                                                >  1:930582/1‑148 (MQ=255)
                       atTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTcaca                                                                                                                              <  1:3031713/148‑1 (MQ=255)
                         tGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGa                                                                                                                            >  2:3248492/1‑148 (MQ=255)
                           cGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAg                                                                                                                          >  2:721610/1‑148 (MQ=255)
                            gTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAgg                                                                                                                         >  1:2068037/1‑148 (MQ=255)
                            gTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAgg                                                                                                                         <  2:2523049/148‑1 (MQ=255)
                              tcttcCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGaa                                                                                                                       >  2:2941955/1‑148 (MQ=255)
                               cttcCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAAt                                                                                                                      >  2:1109737/1‑148 (MQ=255)
                                ttcCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATa                                                                                                                     <  2:2794320/148‑1 (MQ=255)
                                ttcCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATa                                                                                                                     >  1:1692166/1‑148 (MQ=255)
                                ttcCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATAAGCGCCGATGACATTCACAGAAGGAATa                                                                                                                     >  1:1987494/1‑148 (MQ=255)
                                 tcCAGTGCTTTTTGCACATCGCCCGCCATACCTATTTTCGTTAACATATTTTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCATTGAATCAGCGCCGATGACATTCACAGAAGGAATaa                                                                                                                    <  1:2303816/148‑1 (MQ=255)
                                   cAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAg                                                                                                                  >  2:1407544/1‑148 (MQ=255)
                                   cAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAg                                                                                                                  >  2:2602286/1‑148 (MQ=255)
                                     gTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGaa                                                                                                                >  1:1051672/1‑148 (MQ=255)
                                      tGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAAc                                                                                                               >  2:1033553/1‑148 (MQ=255)
                                      tGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAAc                                                                                                               <  1:814453/148‑1 (MQ=255)
                                          ttttGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTg                                                                                                           >  1:1864201/1‑148 (MQ=255)
                                            ttGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGACTAAAGAACGTTGaa                                                                                                         >  1:2855786/1‑148 (MQ=255)
                                                 caTCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATATGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGc                                                                                                    <  2:3283221/148‑1 (MQ=255)
                                                    cgcccgccATACCTAATTTCGTTAACATATTGTCAGTTACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCAt                                                                                                 <  1:2813159/148‑1 (MQ=255)
                                                     gcccgccATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATa                                                                                                <  2:3461684/148‑1 (MQ=255)
                                                        cgccATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAaca                                                                                             >  1:339265/1‑148 (MQ=255)
                                                         gccATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAacac                                                                                            <  1:2190193/148‑1 (MQ=255)
                                                           cATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAacacac                                                                                          >  1:1165667/1‑148 (MQ=255)
                                                             tACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTc                                                                                        <  2:465015/148‑1 (MQ=255)
                                                              aCCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTcc                                                                                       >  1:2227559/1‑148 (MQ=255)
                                                                 tAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGa                                                                                    >  1:863462/1‑148 (MQ=255)
                                                                  aaTTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGaa                                                                                   >  2:2636404/1‑148 (MQ=255)
                                                                   aTTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGaaa                                                                                  >  2:2248344/1‑148 (MQ=255)
                                                                    tttCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAg                                                                                 >  2:231535/1‑148 (MQ=255)
                                                                    tttCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAg                                                                                 >  1:250595/1‑148 (MQ=255)
                                                                        gTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTa                                                                             >  2:754044/1‑148 (MQ=255)
                                                                            aCATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTaaaaa                                                                         <  2:3113336/148‑1 (MQ=255)
                                                                            aCATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTaaaaa                                                                         <  1:3462816/148‑1 (MQ=255)
                                                                             cATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTaaaaaa                                                                        >  1:1577172/1‑148 (MQ=255)
                                                                             cATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTaaaaaa                                                                        >  1:3339434/1‑148 (MQ=255)
                                                                               tatTGTCAGTGAAAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAgg                                                                      <  1:2756125/148‑1 (MQ=255)
                                                                                atTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAggg                                                                     <  2:1142250/148‑1 (MQ=255)
                                                                                atTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAggg                                                                     >  1:3136734/1‑148 (MQ=255)
                                                                                       gTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAgggcgggccg                                                              >  1:2147508/1‑146 (MQ=255)
                                                                                        tGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGc                                                             >  2:1933987/1‑148 (MQ=255)
                                                                                               tAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGa                                                      >  2:318405/1‑148 (MQ=255)
                                                                                                aaGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAg                                                     >  2:1818920/1‑148 (MQ=255)
                                                                                                aaGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAg                                                     <  1:1749132/148‑1 (MQ=255)
                                                                                                aaGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAg                                                     >  2:1230091/1‑148 (MQ=255)
                                                                                                 aGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACANAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAgg                                                    <  1:1803334/148‑1 (MQ=255)
                                                                                                 aGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAgg                                                    <  2:848395/148‑1 (MQ=255)
                                                                                                 aGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCAATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAgg                                                    >  2:550881/1‑148 (MQ=255)
                                                                                                  ggTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGa                                                   >  1:498166/1‑148 (MQ=255)
                                                                                                   gTACGGGTAAATCCATAATCTGCCATCATATTCAGTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGAt                                                  <  2:2748947/148‑1 (MQ=255)
                                                                                                       gggTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTc                                              <  1:10079/148‑1 (MQ=255)
                                                                                                        ggTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCa                                             <  1:978187/148‑1 (MQ=255)
                                                                                                        ggTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCa                                             <  1:1134382/148‑1 (MQ=255)
                                                                                                         gTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCAt                                            <  2:212403/148‑1 (MQ=255)
                                                                                                         gTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCAt                                            <  1:3107096/148‑1 (MQ=255)
                                                                                                         gTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCAt                                            >  1:3502114/1‑148 (MQ=255)
                                                                                                          tAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCAtt                                           >  2:663741/1‑148 (MQ=255)
                                                                                                          tAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCAtt                                           <  2:336754/148‑1 (MQ=255)
                                                                                                          tAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCAtt                                           >  2:1728346/1‑148 (MQ=255)
                                                                                                          tAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCAtt                                           <  2:377282/148‑1 (MQ=255)
                                                                                                                 aTAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAat                                    <  1:2643573/148‑1 (MQ=255)
                                                                                                                 aTAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAat                                    >  2:1889133/1‑148 (MQ=255)
                                                                                                                     tCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGa                                <  1:726359/148‑1 (MQ=255)
                                                                                                                       tGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATc                              <  1:1105924/148‑1 (MQ=255)
                                                                                                                        gCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCa                             >  2:1344243/1‑148 (MQ=255)
                                                                                                                        gCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCa                             >  1:1495013/1‑148 (MQ=255)
                                                                                                                         ccATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAg                            >  1:664502/1‑148 (MQ=255)
                                                                                                                         ccATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAg                            <  1:1731487/148‑1 (MQ=255)
                                                                                                                            tcatATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGtat                         <  2:2674176/148‑1 (MQ=255)
                                                                                                                               tatTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGtattat                      <  2:1635569/148‑1 (MQ=255)
                                                                                                                                 ttCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTg                    >  2:3040233/1‑148 (MQ=255)
                                                                                                                                  tCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGa                   >  2:2847736/1‑148 (MQ=255)
                                                                                                                                  tCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGa                   <  2:1711619/148‑1 (MQ=255)
                                                                                                                                     ttGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTa                >  1:2598865/1‑148 (MQ=255)
                                                                                                                                      tGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTCTTGATTAt               >  2:2891438/1‑148 (MQ=255)
                                                                                                                                         aTCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCaa            >  2:407163/1‑148 (MQ=255)
                                                                                                                                          tCAGTCAATGAATCAGCGCCGATGACATTCACATAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCaaa           <  2:930582/148‑1 (MQ=255)
                                                                                                                                           cAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCaaaa          <  2:1474792/148‑1 (MQ=255)
                                                                                                                                           cAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCaaaa          <  2:3509659/148‑1 (MQ=255)
                                                                                                                                               cAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTaa      >  1:1460992/1‑148 (MQ=255)
                                                                                                                                                  tGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCt   >  1:2957579/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTA  >  CP009273/3750988‑3751282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A