Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F342 I0 R1	1331	225.9	7641038	95.8%	7320114	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,751,135	G→T	41.2%	S18* (TCA→TAA)	yiaY ←	putative Fe‑containing alcohol dehydrogenase, Pfam00465 family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,751,135	0	G	T	41.2%	729.5 / inf	687	S18* (TCA→TAA)	yiaY	putative Fe‑containing alcohol dehydrogenase, Pfam00465 family
Reads supporting (aligned to +/- strand): ref base G (235/169); new base T (152/131); total (387/300)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01

Only 100 of 693 total aligned reads displayed.

CCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTA  >  CP009273/3750988‑3751282

  atcatAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGaa                                                                                                                                                   <  2:584369/148‑1 (MQ=255)

   tcatAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAAt                                                                                                                                                  <  2:3395419/148‑1 (MQ=255)

    catAAATAACGCTAAAAATATTGCGTTCTTCCACTGCTTTTTGAACATCGCCCGCCATACCTAATTTCGTTAACATATTGTAAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTAATTGCATCAGTCAATGAATc                                                                                                                                                 >  1:770100/1‑148 (MQ=255)

      taaataaCGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAg                                                                                                                                               >  1:2909219/1‑148 (MQ=255)

      taaataaCGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAg                                                                                                                                               >  2:3711235/1‑148 (MQ=255)

       aaataaCGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAgc                                                                                                                                              >  2:1209705/1‑148 (MQ=255)

          taaCGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGcc                                                                                                                                           >  1:2428712/1‑148 (MQ=255)

          taaCGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGcc                                                                                                                                           >  2:1596303/1‑148 (MQ=255)

          taaCGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGcc                                                                                                                                           >  2:2422597/1‑148 (MQ=255)

             cGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGAt                                                                                                                                        <  1:1847239/148‑1 (MQ=255)

              gCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATg                                                                                                                                       <  2:931660/148‑1 (MQ=255)

                 aaaaaTATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACa                                                                                                                                    <  2:2843110/148‑1 (MQ=255)

                   aaaTATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACAtt                                                                                                                                  <  1:745966/148‑1 (MQ=255)

                    aaTATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTc                                                                                                                                 >  2:1058768/1‑148 (MQ=255)

                     atatTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTca                                                                                                                                >  2:3272689/1‑148 (MQ=255)

                     atatTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTca                                                                                                                                >  1:1215588/1‑148 (MQ=255)

                       atTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTcaca                                                                                                                              >  2:2349150/1‑148 (MQ=255)

                         tGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGa                                                                                                                            <  1:2142645/148‑1 (MQ=255)

                          gCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGaa                                                                                                                           <  2:3544493/148‑1 (MQ=255)

                            gTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAgg                                                                                                                         <  1:560639/148‑1 (MQ=255)

                             ttcttcCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGa                                                                                                                        >  2:3132625/1‑148 (MQ=255)

                              tcttcCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGaa                                                                                                                       >  1:3494698/1‑148 (MQ=255)

                              tcttcCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGaa                                                                                                                       >  1:963497/1‑148 (MQ=255)

                                    aGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGa                                                                                                                 <  2:3005430/148‑1 (MQ=255)

                                    aGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGa                                                                                                                 <  1:871496/148‑1 (MQ=255)

                                      tGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAAc                                                                                                               >  1:2568497/1‑148 (MQ=255)

                                      tGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAAc                                                                                                               >  1:650150/1‑148 (MQ=255)

                                      tGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAAc                                                                                                               >  1:3274616/1‑148 (MQ=255)

                                             tGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAg                                                                                                        <  2:722115/148‑1 (MQ=255)

                                             tGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAg                                                                                                        <  2:2468733/148‑1 (MQ=255)

                                             tGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAg                                                                                                        <  1:2450892/148‑1 (MQ=255)

                                                 caTCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGc                                                                                                    >  1:2502896/1‑148 (MQ=255)

                                                    cgcccgccATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCAt                                                                                                 >  1:3659701/1‑148 (MQ=255)

                                                     gcccgccATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATa                                                                                                <  1:831609/148‑1 (MQ=255)

                                                         gccATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAacac                                                                                            <  2:2159561/148‑1 (MQ=255)

                                                           cATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAacacac                                                                                          <  2:3624365/148‑1 (MQ=255)

                                                             tACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTc                                                                                        <  1:3770574/148‑1 (MQ=255)

                                                             tACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTc                                                                                        <  2:3806387/148‑1 (MQ=255)

                                                              aCCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTcc                                                                                       >  2:1900807/1‑148 (MQ=255)

                                                              aCCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAAGAAAGAACGTTGAAGCTGCCATAACACACTcc                                                                                       >  2:2762197/1‑148 (MQ=255)

                                                               ccTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCt                                                                                      <  2:1827975/148‑1 (MQ=255)

                                                               ccTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCt                                                                                      >  2:655713/1‑148 (MQ=255)

                                                                cTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTg                                                                                     >  2:3453551/1‑148 (MQ=255)

                                                                cTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTg                                                                                     >  1:401913/1‑148 (MQ=255)

                                                                      tCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAgtg                                                                               <  1:1895420/148‑1 (MQ=255)

                                                                       cGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAgtgt                                                                              >  1:1544150/1‑148 (MQ=255)

                                                                         ttAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCGCTGAATCAGCGCCGATGACATTCACAGAAGGAAGAAAGAACGGTGAAGCTGCCGGAACACACTCCGGTACGTGTac                                                                            >  1:274814/1‑147 (MQ=255)

                                                                               tatTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAgg                                                                      <  1:3429166/148‑1 (MQ=255)

                                                                                  tGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAgggcg                                                                   <  1:357193/148‑1 (MQ=255)

                                                                                  tGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAgggcg                                                                   >  2:1776886/1‑148 (MQ=255)

                                                                                  tGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAgggcg                                                                   >  1:1067433/1‑148 (MQ=255)

                                                                                  tGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAgggcg                                                                   >  1:531119/1‑148 (MQ=255)

                                                                                     cAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAgggcgggc                                                                >  1:1105454/1‑148 (MQ=255)

                                                                                       gTGACAATTAAGGTACGGGTAACTCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAg                                                              <  2:205140/148‑1 (MQ=255)

                                                                                        tGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGc                                                             <  1:2216564/148‑1 (MQ=255)

                                                                                        tGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGc                                                             <  2:519153/148‑1 (MQ=255)

                                                                                           cAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTa                                                          <  1:1421472/148‑1 (MQ=255)

                                                                                            aaTTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTAc                                                         <  2:2710210/148‑1 (MQ=255)

                                                                                            aaTTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTAc                                                         <  2:3361766/148‑1 (MQ=255)

                                                                                             aTTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACt                                                        >  1:2294083/1‑148 (MQ=255)

                                                                                               tAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGa                                                      <  2:3044877/148‑1 (MQ=255)

                                                                                               tAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGa                                                      <  1:3175809/148‑1 (MQ=255)

                                                                                                 aGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAgg                                                    <  2:476817/148‑1 (MQ=255)

                                                                                                  ggTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGa                                                   <  1:2680342/148‑1 (MQ=255)

                                                                                                     aCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCACTGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGCACGTTGAAGCTTCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGAttt                                                <  2:1687744/148‑1 (MQ=255)

                                                                                                     aCGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGAttt                                                >  2:2125100/1‑148 (MQ=255)

                                                                                                       gggTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTc                                              <  1:1738333/148‑1 (MQ=255)

                                                                                                         gTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCAt                                            <  1:1367203/148‑1 (MQ=255)

                                                                                                         gTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTCAAAAAGGGCGGGCAGCTTACTGAGGATTTTCAt                                            >  2:3220955/1‑148 (MQ=255)

                                                                                                            aaTCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCg                                         >  2:2793767/1‑148 (MQ=255)

                                                                                                               ccATAATCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGaaa                                      >  1:2985901/1‑148 (MQ=255)

                                                                                                               ccATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGaaa                                      >  1:132919/1‑148 (MQ=255)

                                                                                                               ccATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGaaa                                      <  1:45482/148‑1 (MQ=255)

                                                                                                                 aTAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAat                                    >  1:3342977/1‑148 (MQ=255)

                                                                                                                     tCTGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGa                                >  1:1796055/1‑148 (MQ=255)

                                                                                                                     tCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGa                                >  1:1637830/1‑148 (MQ=255)

                                                                                                                     tCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGa                                <  2:3160175/148‑1 (MQ=255)

                                                                                                                       tGCCATCATATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATc                              <  2:869075/148‑1 (MQ=255)

                                                                                                                            tcatATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGtat                         >  1:2038458/1‑148 (MQ=255)

                                                                                                                            tcatATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGtat                         <  1:3697652/148‑1 (MQ=255)

                                                                                                                             catATTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGtatt                        >  2:2306058/1‑148 (MQ=255)

                                                                                                                               tatTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAATAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTAATTCGAAAATATGATCAGtattat                      >  1:1462249/1‑148 (MQ=255)

                                                                                                                               tatTCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTTAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGtattat                      >  1:585923/1‑148 (MQ=255)

                                                                                                                               tatTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGtattat                      >  2:3477829/1‑148 (MQ=255)

                                                                                                                               tactCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGtattat                      <  2:199323/145‑1 (MQ=255)

                                                                                                                                 ttCATTACATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGATGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTg                    >  2:1435123/1‑148 (MQ=255)

                                                                                                                                  tCATTGCATCAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGACAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGa                   <  2:2230636/148‑1 (MQ=255)

                                                                                                                                  tCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAATAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGa                   >  2:777834/1‑148 (MQ=255)

                                                                                                                                  tCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGa                   >  1:3083611/1‑148 (MQ=255)

                                                                                                                                      tGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTAt               >  1:2376440/1‑148 (MQ=255)

                                                                                                                                      tGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTAt               <  2:1508776/148‑1 (MQ=255)

                                                                                                                                        cATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCa             <  1:495558/148‑1 (MQ=255)

                                                                                                                                           cAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCaaaa          <  2:2632338/148‑1 (MQ=255)

                                                                                                                                           cAGTCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCaaaa          <  1:2834541/148‑1 (MQ=255)

                                                                                                                                             gTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAAtt        >  1:3232439/1‑148 (MQ=255)

                                                                                                                                              tCAATTAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTa       <  2:1293800/148‑1 (MQ=255)

                                                                                                                                              tCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTa       >  2:3598421/1‑148 (MQ=255)

                                                                                                                                              tCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTa       <  2:295447/148‑1 (MQ=255)

                                                                                                                                                 aTGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATc    <  1:1013391/148‑1 (MQ=255)

                                                                                                                                                 aTGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATc    >  2:2895858/1‑148 (MQ=255)

CCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCATTGCATCAGTCAATGAATCAGCGCCGATGACATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTA  >  CP009273/3750988‑3751282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A