Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,214,809 G→T 94.5% E352* (GAA→TAA)  metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,214,8090GT94.5% 499.2 / 16.4 189E352* (GAA→TAA) metHhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand):  ref base G (9/3);  new base T (101/76);  total (110/79)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

CTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT  >  CP009273/4214665‑4214952
                                                                                                                                                |                                                                                                                                               
ctcAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAg                                                                                                                                              <  1:665230/148‑1 (MQ=255)
  cAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGAt                                                                                                                                            <  2:1417938/148‑1 (MQ=255)
     tatCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGc                                                                                                                                         <  1:3501713/148‑1 (MQ=255)
     tatCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGc                                                                                                                                         <  1:2217566/148‑1 (MQ=255)
      atCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGcc                                                                                                                                        >  2:1034479/1‑148 (MQ=255)
      atCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATGGCGAAGATAGcc                                                                                                                                        >  1:2131746/1‑148 (MQ=255)
      atCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTGTGTCCGGCCTGGAGCCGCGGAACAATGGGTAAGATATcc                                                                                                                                        >  1:3275444/1‑148 (MQ=255)
       tcgtcgGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGGGCGGCCTGGAGCCGCGGAACAATTGCTAAGATAGCCt                                                                                                                                       >  1:2681658/1‑148 (MQ=255)
          tcgGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGtt                                                                                                                                    >  2:718669/1‑148 (MQ=255)
          tcgGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATTGCTAAGATAGCCTGtt                                                                                                                                    >  1:2555255/1‑148 (MQ=255)
             gcggcTGCTGTGGAACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTtgt                                                                                                                                 <  1:2130071/148‑1 (MQ=255)
                   gctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGt                                                                                                                           <  2:1883607/148‑1 (MQ=255)
                   gctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGt                                                                                                                           <  1:130315/148‑1 (MQ=255)
                    ctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTg                                                                                                                          >  2:28157/1‑148 (MQ=255)
                     tgtgGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTgg                                                                                                                         <  1:580798/148‑1 (MQ=255)
                      gtgGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTggg                                                                                                                        >  2:1298262/1‑148 (MQ=255)
                      gtgGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTggg                                                                                                                        >  1:249070/1‑148 (MQ=255)
                       tgGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGt                                                                                                                       <  2:3312563/148‑1 (MQ=255)
                               cGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCAc                                                                                                               >  2:2523960/1‑148 (MQ=255)
                                   acaacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTTAACGTGGGTGAACGCACCAAc                                                                                                           >  1:3081826/1‑148 (MQ=255)
                                    caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACg                                                                                                          >  1:2191741/1‑148 (MQ=255)
                                       caTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCa                                                                                                       <  1:2532089/148‑1 (MQ=255)
                                       caTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCa                                                                                                       <  1:2532110/148‑1 (MQ=255)
                                       caTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCa                                                                                                       >  2:2736735/1‑148 (MQ=255)
                                         tatTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCAcc                                                                                                     <  1:3301236/148‑1 (MQ=255)
                                         tatTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCAGTTTGTGAACGTGGGTGAAAGCACCAACGTCAcc                                                                                                     >  1:1271064/1‑148 (MQ=255)
                                                 cGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCGGGAGCCGCTGAACAATTGCTAAGATATCCTGTTTTTGAACCGGGGTGAACGCACCAACGGCACCGGTTCCGc                                                                                             >  1:1610367/1‑148 (MQ=255)
                                                  gATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCt                                                                                            <  2:1206395/148‑1 (MQ=255)
                                                    tGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTaa                                                                                          <  1:985787/148‑1 (MQ=255)
                                                     gAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTTTGAAAGTGGGTGAAAGCACCCACGTCACCGGTTCCGCTAAg                                                                                         >  1:385295/1‑148 (MQ=255)
                                                      aGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGGAACCTTTGCTAAGATATCCGGTTTTTGAACCTGGGTGAAACCACCAACCTCACCCGGTCCCCTaatt                                                                                        >  1:2094292/1‑146 (MQ=255)
                                                          gTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCaa                                                                                    <  1:460947/148‑1 (MQ=255)
                                                          gTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCaa                                                                                    <  1:461739/148‑1 (MQ=255)
                                                          gTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCaa                                                                                    <  1:1087853/148‑1 (MQ=255)
                                                            gCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgc                                                                                  >  2:854755/1‑148 (MQ=255)
                                                            gCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgc                                                                                  >  2:854748/1‑148 (MQ=255)
                                                             cAGTAGAAGGATTAGCGCCACGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgcg                                                                                 >  2:991311/1‑148 (MQ=255)
                                                             cAGTAGAAGGATTAGCGCCACGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgcg                                                                                 >  2:2924058/1‑148 (MQ=255)
                                                              aGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgcgc                                                                                <  1:1385943/148‑1 (MQ=255)
                                                              aGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgcgc                                                                                <  2:3100887/148‑1 (MQ=255)
                                                              aGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgcgc                                                                                <  1:376410/148‑1 (MQ=255)
                                                                 aGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTg                                                                             <  2:2322603/148‑1 (MQ=255)
                                                                 aGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTg                                                                             <  2:3483013/148‑1 (MQ=255)
                                                                  gAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAATATATCCTGTTTGTGAAAGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGAGCCTGa                                                                            >  1:2364503/1‑148 (MQ=255)
                                                                  gAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATATCCAGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAACTTCAAGCGCCTGa                                                                            >  1:577772/1‑148 (MQ=255)
                                                                  gAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGa                                                                            <  1:1011691/148‑1 (MQ=255)
                                                                       aTTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCaaa                                                                       >  2:3518662/1‑148 (MQ=255)
                                                                         tAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaaga                                                                     >  2:550177/1‑148 (MQ=255)
                                                                           gcgcCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaagaag                                                                   <  2:3254144/148‑1 (MQ=255)
                                                                            cgccgcGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAagaaga                                                                  <  1:2529889/148‑1 (MQ=255)
                                                                              ccgcGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAgaga                                                                >  1:215273/1‑148 (MQ=255)
                                                                               cgcgCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGaa                                                               <  2:2660715/148‑1 (MQ=255)
                                                                                gcgcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGaaa                                                              <  2:987116/148‑1 (MQ=255)
                                                                                  gcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATa                                                            <  1:1493047/148‑1 (MQ=255)
                                                                                   cAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATAc                                                           <  1:3426892/148‑1 (MQ=255)
                                                                                     aaCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAg                                                         <  1:589852/148‑1 (MQ=255)
                                                                                      aCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGc                                                        >  2:2221486/1‑148 (MQ=255)
                                                                                      aCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGGAACATTTGCTAAGATAGCCGGTTTGTGAACGGGGGTGGACGCACCAACGTCACCGGTTGCGCTAAGTTAAAGCGCCGGATCAAATAAGAGAAAAAAAGc                                                        >  1:759080/1‑148 (MQ=255)
                                                                                       cTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCg                                                       >  2:2052672/1‑148 (MQ=255)
                                                                                       cTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCg                                                       >  2:1628886/1‑148 (MQ=255)
                                                                                           cGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGgc                                                   <  2:1513702/148‑1 (MQ=255)
                                                                                              aaaTTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGGTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCt                                                >  1:2896066/1‑148 (MQ=255)
                                                                                                aTTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTgg                                              <  1:1484364/148‑1 (MQ=255)
                                                                                                aTTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTgg                                              <  2:614020/148‑1 (MQ=255)
                                                                                                 ttCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGATAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGa                                             <  1:1648714/148‑1 (MQ=255)
                                                                                                    ccGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGt                                          <  1:1349614/148‑1 (MQ=255)
                                                                                                      ggaGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTcg                                        <  2:2381865/146‑1 (MQ=255)
                                                                                                          ccTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTcgcgcg                                    <  2:428764/148‑1 (MQ=255)
                                                                                                           cTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGt                                   <  2:1639202/148‑1 (MQ=255)
                                                                                                           cTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGt                                   <  2:1639216/148‑1 (MQ=255)
                                                                                                            tGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGTGTc                                  >  1:2926120/1‑148 (MQ=255)
                                                                                                              ccGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCaa                                >  1:1709465/1‑148 (MQ=255)
                                                                                                              ccGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCaa                                >  2:249228/1‑148 (MQ=255)
                                                                                                              ccGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCaa                                >  2:2722552/1‑148 (MQ=255)
                                                                                                                gTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACa                              <  1:693072/148‑1 (MQ=255)
                                                                                                                gTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACa                              >  2:1365842/1‑148 (MQ=255)
                                                                                                                gTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACa                              >  2:1365923/1‑148 (MQ=255)
                                                                                                                gTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACa                              >  1:190174/1‑148 (MQ=255)
                                                                                                                 tttGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAg                             >  2:1714865/1‑148 (MQ=255)
                                                                                                                  ttGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAgg                            >  2:1991676/1‑148 (MQ=255)
                                                                                                                  ttGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAgg                            >  2:1786667/1‑148 (MQ=255)
                                                                                                                  ttGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAgg                            >  2:2500207/1‑148 (MQ=255)
                                                                                                                    gTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTg                          <  2:2547072/148‑1 (MQ=255)
                                                                                                                            tGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACgg                  >  2:710592/1‑148 (MQ=255)
                                                                                                                             ggAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgc                 >  2:1918308/1‑148 (MQ=255)
                                                                                                                             ggAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgc                 >  2:3365550/1‑148 (MQ=255)
                                                                                                                             ggAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgc                 >  1:1756731/1‑148 (MQ=255)
                                                                                                                              gAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcg                <  1:1165397/148‑1 (MQ=255)
                                                                                                                                gCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgcg              >  1:3036040/1‑148 (MQ=255)
                                                                                                                                gCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgcg              >  1:2451348/1‑148 (MQ=255)
                                                                                                                                 ccGCTGAACATTGGCTAAGCTAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgcgc             <  2:2210991/148‑1 (MQ=255)
                                                                                                                                   gCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAg           >  1:644038/1‑148 (MQ=255)
                                                                                                                                    cTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCTTCAACAGGTGGAAAACGGCGCGCAGa          >  1:1101541/1‑148 (MQ=255)
                                                                                                                                    cTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGa          >  1:3219184/1‑148 (MQ=255)
                                                                                                                                    cTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGa          >  1:3215390/1‑148 (MQ=255)
                                                                                                                                    cTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGa          <  2:1943857/148‑1 (MQ=255)
                                                                                                                                     tGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGAt         <  1:2523840/148‑1 (MQ=255)
                                                                                                                                            tGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGat  >  1:852420/1‑148 (MQ=255)
                                                                                                                                            tGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGat  >  1:2633761/1‑148 (MQ=255)
                                                                                                                                            tGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGat  >  1:1105190/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT  >  CP009273/4214665‑4214952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCA  >  CP009273/4214665‑4214956
                                                                                                                                                |                                                                                                                                                   
CTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAAGATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGAT                                                                                                                                                <  SRR19973067.669286/150‑1 (MQ=60)
CTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGAT                                                                                                                                                <  SRR19973067.670164/150‑1 (MQ=60)
CTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGAT                                                                                                                                                <  SRR19973067.669775/150‑1 (MQ=60)
    ATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGTACATTGGCGCAGATAGCC                                                                                                                                            >  SRR19973067.2145970/1‑150 (MQ=60)
    ATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACCTTTGCTAAGATAGCC                                                                                                                                            >  SRR19973067.3295946/1‑150 (MQ=60)
    ATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATGGCGAAGATAGCC                                                                                                                                            >  SRR19973067.2145981/1‑150 (MQ=60)
     TATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCT                                                                                                                                           <  SRR19973067.2232334/150‑1 (MQ=60)
     TATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCT                                                                                                                                           <  SRR19973067.3523911/150‑1 (MQ=60)
     TATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCT                                                                                                                                           <  SRR19973067.2232315/150‑1 (MQ=60)
     TATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCT                                                                                                                                           <  SRR19973067.2232583/150‑1 (MQ=60)
     TATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGGGCGGCCTGGAGCCGCGGAACAATTGCTAAGATAGCCT                                                                                                                                           >  SRR19973067.2698842/1‑150 (MQ=60)
        CGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATTGCTAAGATAGCCTGTT                                                                                                                                        >  SRR19973067.2571964/1‑150 (MQ=60)
        CGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATGGCTAAGATAGCCTGTT                                                                                                                                        >  SRR19973067.3084293/1‑150 (MQ=60)
             GCGGCTGCTGTGGAACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGA                                                                                                                                   <  SRR19973067.3549319/150‑1 (MQ=60)
             GCGGCTGCTGTGGAACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGA                                                                                                                                   <  SRR19973067.2144289/150‑1 (MQ=60)
                GCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACG                                                                                                                                <  SRR19973067.3424183/150‑1 (MQ=60)
                   GCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGG                                                                                                                             <  SRR19973067.3048162/150‑1 (MQ=60)
                   GCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGG                                                                                                                             <  SRR19973067.131499/150‑1 (MQ=60)
                    CTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGG                                                                                                                            >  SRR19973067.250791/1‑150 (MQ=60)
                     TGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGT                                                                                                                           <  SRR19973067.584109/150‑1 (MQ=60)
                     TGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGT                                                                                                                           <  SRR19973067.584524/150‑1 (MQ=60)
                                 CCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTTAACGTGGGTGAACGCACCAAC                                                                                                               >  SRR19973067.3101438/1‑150 (MQ=60)
                                 CCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAAC                                                                                                               <  SRR19973067.1110028/150‑1 (MQ=60)
                                 CCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCGGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGGGAACGCCCCCAC                                                                                                               >  SRR19973067.3101439/1‑150 (MQ=60)
                                  CACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACG                                                                                                              >  SRR19973067.2206368/1‑150 (MQ=60)
                                       CATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACC                                                                                                         >  SRR19973067.1279874/1‑150 (MQ=60)
                                       CATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACC                                                                                                         <  SRR19973067.2548677/150‑1 (MQ=60)
                                       CATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACC                                                                                                         <  SRR19973067.2549519/150‑1 (MQ=60)
                                       CATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACC                                                                                                         <  SRR19973067.2548698/150‑1 (MQ=60)
                                       CATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCAGTTTGTGAACGTGGGTGAAAGCACCAACGTCACC                                                                                                         >  SRR19973067.1279707/1‑150 (MQ=60)
                                         TATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGG                                                                                                       <  SRR19973067.3322034/150‑1 (MQ=60)
                                             GCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCC                                                                                                   <  SRR19973067.2586851/150‑1 (MQ=60)
                                               AGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCGGGAGCCGCTGAACAATTGCTAAGATATCCTGTTTTTGAACCGGGGTGAACGCACCAACGGCACCGGTTCCGC                                                                                                 >  SRR19973067.1621426/1‑150 (MQ=60)
                                                   ATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTTTGAAAGTGGGTGAAAGCACCCACGTCACCGGTTCCGCTAAG                                                                                             >  SRR19973067.387982/1‑150 (MQ=60)
                                                   ATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACCTCACCGGTTCCGCTAAG                                                                                             >  SRR19973067.388003/1‑150 (MQ=60)
                                                   ATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTGTGTCCGGCCTGGAGCCGCTGAACAATGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAG                                                                                             >  SRR19973067.387968/1‑150 (MQ=60)
                                                    TGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGT                                                                                            <  SRR19973067.992567/150‑1 (MQ=60)
                                                    TGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGGAACCTTTGCTAAGATATCCGGTTTTTGAACCTGGGTGAAaccaccaacctcacccggtcccctaatt                                                                                            >  SRR19973067.2108146/1‑122 (MQ=60)
                                                      AGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTC                                                                                          <  SRR19973067.1377201/150‑1 (MQ=60)
                                                          GTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGC                                                                                      <  SRR19973067.464137/150‑1 (MQ=60)
                                                          GTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGC                                                                                      <  SRR19973067.1095286/150‑1 (MQ=60)
                                                          GTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGC                                                                                      <  SRR19973067.464849/150‑1 (MQ=60)
                                                          GTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGC                                                                                      <  SRR19973067.464935/150‑1 (MQ=60)
                                                              AGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCT                                                                                  <  SRR19973067.379027/150‑1 (MQ=60)
                                                              AGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCT                                                                                  <  SRR19973067.1395374/150‑1 (MQ=60)
                                                              AGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCT                                                                                  <  SRR19973067.2115328/150‑1 (MQ=60)
                                                                TAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAATATATCCTGTTTGTGAAAGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGAGCCTGA                                                                                >  SRR19973067.2379904/1‑150 (MQ=60)
                                                                TAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATATCCAGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAACTTCAAGCGCCTGA                                                                                >  SRR19973067.581487/1‑150 (MQ=60)
                                                                TAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATGGCTAAGATAGCCTGTTTGTGAACCTGGGTGAACGCACCAACGTCACCCGTTCCGCTAAGTTCAAGCGCCGGA                                                                                >  SRR19973067.2498739/1‑150 (MQ=60)
                                                                  GAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATC                                                                              <  SRR19973067.1018639/150‑1 (MQ=60)
                                                                         TAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAG                                                                       <  SRR19973067.2656911/150‑1 (MQ=60)
                                                                            CGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGA                                                                    <  SRR19973067.2546493/150‑1 (MQ=60)
                                                                            CGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGA                                                                    <  SRR19973067.2546465/150‑1 (MQ=60)
                                                                            CGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGA                                                                    >  SRR19973067.216865/1‑150 (MQ=60)
                                                                               CGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAAT                                                                 <  SRR19973067.327694/150‑1 (MQ=60)
                                                                                  GCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACA                                                              <  SRR19973067.3491123/150‑1 (MQ=60)
                                                                                  GCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACA                                                              <  SRR19973067.1502911/150‑1 (MQ=60)
                                                                                   CAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAG                                                             <  SRR19973067.3448701/150‑1 (MQ=60)
                                                                                    AAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATATCCTGTTTGTGAACGTGGGTGAACGCACCCACGGCACCCGTTCCGCTAAGTTCAAGCGCCGGATCAAAGAAGAGAAATACAGC                                                            >  SRR19973067.764346/1‑150 (MQ=60)
                                                                                    AAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGGAACATTTGCTAAGATAGCCGGTTTGTGAACGGGGGTGGACGCACCAACGTCACCGGTTGCGCTAAGTTAAAGCGCCGGATCAAATAAGAGAAAAAAAGC                                                            >  SRR19973067.764353/1‑150 (MQ=60)
                                                                                     AACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCG                                                           <  SRR19973067.593611/150‑1 (MQ=60)
                                                                                     AACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCGGGAGCCGCTGTACATTGGCTAAGATAGCCTGTTTGTGTACGTGGGTGAACGCACCCACCTCACCCGTTCCGCTAAGTTCAAGCGCCGGATAAAATAAGAGAAATAAAGCG                                                           >  SRR19973067.2183248/1‑150 (MQ=60)
                                                                                            GGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCGGATCAAAGAAGAGAAATACAGCGAGGCGCG                                                    >  SRR19973067.1129192/1‑150 (MQ=60)
                                                                                            GGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGGTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCT                                                    >  SRR19973067.2914551/1‑150 (MQ=60)
                                                                                               AATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAAAATTGGCGAAGAAAGCCTGTTTGTGAACCTGGGTGAACGCACCAACCTCACCCGTTCCGCTAAGTTAAACCGCCAGATCAAAGAAGAGAAATACAACCAGGCGCTGGA                                                 >  SRR19973067.246607/1‑150 (MQ=60)
                                                                                               AATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGGAACATTGGCTAAGATATCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCCCCGGTTCCGCGAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCgggc                                                 >  SRR19973067.2839662/1‑146 (MQ=60)
                                                                                                ATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGTACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTGAAGCCCCGGATCAAAGAAGAGAAAAAAACCGAGGCGCTGGGT                                                >  SRR19973067.2524060/1‑150 (MQ=60)
                                                                                                ATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGAT                                                <  SRR19973067.975499/150‑1 (MQ=60)
                                                                                                ATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGAT                                                <  SRR19973067.1323956/150‑1 (MQ=60)
                                                                                                ATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGAT                                                <  SRR19973067.1494205/150‑1 (MQ=60)
                                                                                                ATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAAAAATGGCGAAGATAGACTGTTTTTTAAAGTGGGTGGACGCACCCACCTCACCGGGTGCGCGAAGTTCAAGCGCCAGATAAAAGAATAGAAAAAAAGCGAGGCGCGGGAT                                                >  SRR19973067.646907/1‑150 (MQ=53)
                                                                                                 TTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGATAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATG                                               <  SRR19973067.1660071/150‑1 (MQ=60)
                                                                                                 TTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGATAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATG                                               <  SRR19973067.1659761/150‑1 (MQ=60)
                                                                                                    CCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCG                                            <  SRR19973067.1358841/150‑1 (MQ=60)
                                                                                                       TAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACCTCACCCGTTCCGCTAAGTTAAAGCGCCTGATAAAAGAAGAGAAATACAGCGAGGCGCAGGATGTCGCGC                                         >  SRR19973067.2049945/1‑150 (MQ=60)
                                                                                                          CCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATAAAAGAAGAGAAAAAAAGCGAGGCGCTGGATGTCGCGCGTC                                      >  SRR19973067.1436140/1‑150 (MQ=60)
                                                                                                          CCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATAAAGCGAGGCGCTGGATGTCGCGTGTC                                      >  SRR19973067.2944713/1‑150 (MQ=60)
                                                                                                            TGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAA                                    >  SRR19973067.1721193/1‑150 (MQ=60)
                                                                                                              CCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACA                                  >  SRR19973067.191649/1‑150 (MQ=60)
                                                                                                                GTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGG                                <  SRR19973067.697851/150‑1 (MQ=60)
                                                                                                                GTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGG                                >  SRR19973067.1787217/1‑150 (MQ=60)
                                                                                                                     TCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAA                           >  SRR19973067.810948/1‑150 (MQ=60)
                                                                                                                           CTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGC                     >  SRR19973067.1768696/1‑150 (MQ=60)
                                                                                                                              GAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCG                  <  SRR19973067.1173290/150‑1 (MQ=60)
                                                                                                                              GAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCG                  >  SRR19973067.3055381/1‑150 (MQ=60)
                                                                                                                              GAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCG                  <  SRR19973067.1173143/150‑1 (MQ=60)
                                                                                                                              GAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCG                  >  SRR19973067.2467460/1‑150 (MQ=60)
                                                                                                                                 CCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAG               >  SRR19973067.648388/1‑150 (MQ=60)
                                                                                                                                  CGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCTTCAACAGGTGGAAAACGGCGCGCAGA              >  SRR19973067.1109034/1‑150 (MQ=60)
                                                                                                                                  CGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCTTCAACAGGTGGAAAACGGCGCGCAGA              >  SRR19973067.1108939/1‑150 (MQ=60)
                                                                                                                                  CGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGA              >  SRR19973067.3235626/1‑150 (MQ=60)
                                                                                                                                  CGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGA              >  SRR19973067.3239430/1‑150 (MQ=60)
                                                                                                                                   GCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGAT             >  SRR19973067.2934480/1‑150 (MQ=60)
                                                                                                                                     TGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTA           <  SRR19973067.2540503/150‑1 (MQ=60)
                                                                                                                                     TGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTA           <  SRR19973067.2540383/150‑1 (MQ=60)
                                                                                                                                         CATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGA       >  SRR19973067.585402/1‑150 (MQ=60)
                                                                                                                                         CATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGA       >  SRR19973067.600126/1‑150 (MQ=60)
                                                                                                                                          ATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT      >  SRR19973067.858175/1‑150 (MQ=60)
                                                                                                                                          ATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT      >  SRR19973067.175947/1‑150 (MQ=60)
                                                                                                                                          ATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT      >  SRR19973067.2650804/1‑150 (MQ=60)
                                                                                                                                          ATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT      >  SRR19973067.1112699/1‑150 (MQ=60)
                                                                                                                                          ATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT      >  SRR19973067.1586008/1‑150 (MQ=60)
                                                                                                                                           TTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATA     <  SRR19973067.1702922/150‑1 (MQ=60)
                                                                                                                                              GCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCA  <  SRR19973067.100171/150‑1 (MQ=60)
                                                                                                                                              GCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCA  <  SRR19973067.100123/150‑1 (MQ=60)
                                                                                                                                              GCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATAGATATCA  <  SRR19973067.2236272/150‑1 (MQ=60)
                                                                                                                                                |                                                                                                                                                   
CTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCA  >  CP009273/4214665‑4214956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑