Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,214,809 G→T 100% E352* (GAA→TAA)  metH → homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,214,8090GT97.2% 509.6 / ‑0.5 190E352* (GAA→TAA) metHhomocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand):  ref base G (10/0);  new base T (93/87);  total (103/87)
Fisher's exact test for biased strand distribution p-value = 2.08e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

CTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATA  >  CP009273/4214665‑4214953
                                                                                                                                                |                                                                                                                                                
  cAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGAt                                                                                                                                             <  1:1612363/148‑1 (MQ=255)
  cAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGAt                                                                                                                                             >  2:1798083/1‑148 (MQ=255)
     tatCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGc                                                                                                                                          <  2:1832526/148‑1 (MQ=255)
       tcgtcgGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCt                                                                                                                                        <  2:280101/148‑1 (MQ=255)
              cggcTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTtgtg                                                                                                                                 <  1:448728/148‑1 (MQ=255)
                  tgctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACg                                                                                                                             >  1:2529965/1‑148 (MQ=255)
                    ctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTg                                                                                                                           <  2:3045643/148‑1 (MQ=255)
                       tgGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGt                                                                                                                        <  2:2625128/148‑1 (MQ=255)
                        ggCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTg                                                                                                                       <  1:1771357/148‑1 (MQ=255)
                          caccacGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCAGTTTTTGTACGTGGGTGaa                                                                                                                     >  1:1589412/1‑148 (MQ=255)
                           accacGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAAc                                                                                                                    >  1:1349298/1‑148 (MQ=255)
                            ccacGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGGTTGTGAACGTGGGTGAACg                                                                                                                   >  1:52874/1‑148 (MQ=255)
                            ccacGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGGAACATTGGGGAAGATATCCTGTTTGTGAAAGTGGGTGTACg                                                                                                                   >  1:1530213/1‑148 (MQ=255)
                             cacGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGc                                                                                                                  >  1:2863464/1‑148 (MQ=255)
                             cacGCCACAACATAGTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGc                                                                                                                  <  1:1964011/148‑1 (MQ=255)
                               cGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCAc                                                                                                                >  2:2160511/1‑148 (MQ=255)
                                 ccACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCa                                                                                                              <  2:2952656/148‑1 (MQ=255)
                                  cacaACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCaa                                                                                                             <  1:1631116/148‑1 (MQ=255)
                                  cacaACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCaa                                                                                                             >  2:856125/1‑148 (MQ=255)
                                    caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTGTGAAAGTGGGTGAACGCACCAACg                                                                                                           >  1:2812243/1‑148 (MQ=255)
                                    caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACg                                                                                                           <  1:3256692/148‑1 (MQ=255)
                                    caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACg                                                                                                           >  2:778862/1‑148 (MQ=255)
                                    caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACg                                                                                                           <  1:2266883/148‑1 (MQ=255)
                                        atatTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCAc                                                                                                       >  1:2309999/1‑148 (MQ=255)
                                         tatTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCAcc                                                                                                      >  2:1258609/1‑148 (MQ=255)
                                         tatTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCAcc                                                                                                      <  1:2226545/148‑1 (MQ=255)
                                            tGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGt                                                                                                   <  2:1553532/148‑1 (MQ=255)
                                             gCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGtt                                                                                                  <  2:2045374/148‑1 (MQ=255)
                                                 cGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATATCCTGTTTGTGAACGTGGGTGAACGCACCCACGTCACCCGGTCCGc                                                                                              >  1:3005084/1‑148 (MQ=255)
                                                 cGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGc                                                                                              >  1:2068771/1‑148 (MQ=255)
                                                 cGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGc                                                                                              >  1:1190796/1‑148 (MQ=255)
                                                 cGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATATCCTGTTTGTGTACGTGGGTGAACGCACCCACCGCACCCGTTCCGc                                                                                              >  1:677064/1‑148 (MQ=255)
                                                  gATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCt                                                                                             <  2:1179623/148‑1 (MQ=255)
                                                   ctGAGGCGTGCAGTAGAAGGATTAGCGCCGCGCACACTGCCGGAAATTCGCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTa                                                                                            <  2:1855764/147‑1 (MQ=255)
                                                   aTGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTa                                                                                            <  1:2900127/148‑1 (MQ=255)
                                                   aTGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTa                                                                                            >  1:1915785/1‑148 (MQ=255)
                                                     gAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCGAAGATATCCTGTTTTTGAACGTGGGTGAACGCACCCACCTCACCCGTTCCGCTAAg                                                                                          >  1:2169178/1‑148 (MQ=255)
                                                     gAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATATCCTGTTTGTGAACGTGGGTGAACGCCCCAACGTCACCGGTTCCGCTAAg                                                                                          >  1:2582093/1‑148 (MQ=255)
                                                     gAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAg                                                                                          >  1:2582023/1‑148 (MQ=255)
                                                         cGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCa                                                                                      <  2:2303781/148‑1 (MQ=255)
                                                          gTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCaa                                                                                     <  2:460768/148‑1 (MQ=255)
                                                            gCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGGTCCGCTAAGTTCAAgc                                                                                   >  1:1996466/1‑148 (MQ=255)
                                                              aGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgcgc                                                                                 <  2:1657199/148‑1 (MQ=255)
                                                               gTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGcc                                                                                <  2:1751767/148‑1 (MQ=255)
                                                               gTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGcc                                                                                <  2:1343721/148‑1 (MQ=255)
                                                                tAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCt                                                                               >  2:253201/1‑148 (MQ=255)
                                                                tAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCCGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCt                                                                               >  2:2414133/1‑148 (MQ=255)
                                                                 aGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTg                                                                              >  1:2360616/1‑148 (MQ=255)
                                                                   aaGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGAt                                                                            >  1:1044803/1‑148 (MQ=255)
                                                                     ggATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCa                                                                          >  1:2090052/1‑148 (MQ=255)
                                                                         tAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaaga                                                                      <  2:3381383/148‑1 (MQ=255)
                                                                          aGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaagaa                                                                     <  1:1349989/148‑1 (MQ=255)
                                                                           gcgcCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaagaag                                                                    >  1:1482010/1‑148 (MQ=255)
                                                                           gcgcCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaagaag                                                                    <  1:1399472/148‑1 (MQ=255)
                                                                              ccgcGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAgaga                                                                 <  1:1547594/148‑1 (MQ=255)
                                                                                 cgcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAAt                                                              >  2:280647/1‑148 (MQ=255)
                                                                                 cgcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAAt                                                              >  2:279644/1‑148 (MQ=255)
                                                                                 cgcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAAt                                                              <  1:679514/148‑1 (MQ=255)
                                                                                 cgcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAAt                                                              >  2:3410069/1‑148 (MQ=255)
                                                                                   cAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATAc                                                            <  2:3037379/148‑1 (MQ=255)
                                                                                   cAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATGGCGAAGATAGCCTGTTTGTGTACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATAc                                                            >  1:3487866/1‑148 (MQ=255)
                                                                                      aCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGc                                                         >  2:1594733/1‑148 (MQ=255)
                                                                                       cTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCg                                                        >  2:223437/1‑148 (MQ=255)
                                                                                          ccGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAgg                                                     <  2:2836450/148‑1 (MQ=255)
                                                                                            ggAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGgcg                                                   <  1:3157978/148‑1 (MQ=255)
                                                                                             gAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGgcgc                                                  >  2:1398967/1‑148 (MQ=255)
                                                                                             gAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGgcgc                                                  >  2:690810/1‑148 (MQ=255)
                                                                                              aaaTTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCt                                                 >  2:380785/1‑148 (MQ=255)
                                                                                                   cccGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATg                                            <  1:1311220/148‑1 (MQ=255)
                                                                                                   cccGGAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATg                                            <  2:2736672/148‑1 (MQ=255)
                                                                                                    ccGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGt                                           >  1:3545691/1‑148 (MQ=255)
                                                                                                     cGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAAAAATTGGGAACATATCCTTTTTGTGAAAGTTGGTGAACGCACCAAACGCACCCGTTCCGCTAAGTTAAAGCGCCTGATCAAAGAAAAGAAATACAGCGAGGCGCTgggtttg                                          >  1:80726/1‑143 (MQ=255)
                                                                                                          ccTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTcgcgcg                                     >  2:1924445/1‑148 (MQ=255)
                                                                                                          ccTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTcgcgcg                                     <  1:2664635/148‑1 (MQ=255)
                                                                                                           cTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGt                                    <  1:2883066/148‑1 (MQ=255)
                                                                                                           cTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGt                                    <  2:953098/148‑1 (MQ=255)
                                                                                                           cTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGt                                    <  1:1201747/148‑1 (MQ=255)
                                                                                                            tGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATGGCGAAGATATCCTGTTTGTTAACGTGGGGGAACGCACCAACCTCACCCGTTCCGCGAAGTTCAAACCCCGGATAAAATAAGAGAAAAAAACCGAGGCGCTGGATGTCGCGCGTc                                   >  1:140285/1‑148 (MQ=255)
                                                                                                              ccGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCCCCCGTTCCGCGAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACATCGAGGCGCTGGATGTCGCGCGTCaa                                 >  1:3364792/1‑148 (MQ=255)
                                                                                                              ccGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCaa                                 <  2:2169178/148‑1 (MQ=255)
                                                                                                               cGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAAc                                <  1:1123361/148‑1 (MQ=255)
                                                                                                                  ttGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAgg                             >  2:357696/1‑148 (MQ=255)
                                                                                                                            tGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACgg                   <  1:279644/148‑1 (MQ=255)
                                                                                                                            tGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACgg                   <  1:280647/148‑1 (MQ=255)
                                                                                                                            tGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACgg                   <  1:3410069/148‑1 (MQ=255)
                                                                                                                             ggAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgc                  >  2:3319801/1‑148 (MQ=255)
                                                                                                                              gAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcg                 >  1:663623/1‑148 (MQ=255)
                                                                                                                               aGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgc                >  2:2747121/1‑148 (MQ=255)
                                                                                                                               aGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgc                >  2:2747124/1‑148 (MQ=255)
                                                                                                                                gCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgcg               >  2:2455466/1‑148 (MQ=255)
                                                                                                                                gCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgcg               >  2:748293/1‑148 (MQ=255)
                                                                                                                                   gCTGAACTTTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAg            <  1:2414152/148‑1 (MQ=255)
                                                                                                                                   gCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACAAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAg            <  1:3189819/148‑1 (MQ=255)
                                                                                                                                     tGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGAt          >  1:1604404/1‑148 (MQ=255)
                                                                                                                                         cATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATc      >  1:1619692/1‑148 (MQ=255)
                                                                                                                                           ttGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGa    >  2:2378513/1‑148 (MQ=255)
                                                                                                                                            tGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGat   >  1:916765/1‑148 (MQ=255)
                                                                                                                                            tGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGat   >  1:3195047/1‑148 (MQ=255)
                                                                                                                                             ggCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATCCAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGata  <  2:146945/148‑1 (MQ=255)
                                                                                                                                             ggCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGata  <  2:1731495/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATA  >  CP009273/4214665‑4214953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCA  >  CP009273/4214665‑4214956
                                                                                                                                                |                                                                                                                                                   
CTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGAT                                                                                                                                                <  SRR19973066.3344390/150‑1 (MQ=60)
  CAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAG                                                                                                                                              <  SRR19973066.1624058/150‑1 (MQ=60)
  CAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAG                                                                                                                                              <  SRR19973066.1624029/150‑1 (MQ=60)
  CAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAG                                                                                                                                              >  SRR19973066.1411380/1‑150 (MQ=60)
      ATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATATCCTG                                                                                                                                          >  SRR19973066.1952900/1‑150 (MQ=60)
         GTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTT                                                                                                                                       <  SRR19973066.2290375/150‑1 (MQ=60)
              CGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAA                                                                                                                                  <  SRR19973066.452203/150‑1 (MQ=60)
              CGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAA                                                                                                                                  <  SRR19973066.452185/150‑1 (MQ=60)
              CGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCGGGAGCCGCGGAACCTTGGCTAAGATAGCCGGTTTGTGTA                                                                                                                                  >  SRR19973066.1359413/1‑150 (MQ=60)
                GCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTGTGAACG                                                                                                                                >  SRR19973066.2547453/1‑150 (MQ=60)
                GCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACG                                                                                                                                >  SRR19973066.2547167/1‑150 (MQ=60)
                GCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACG                                                                                                                                >  SRR19973066.1780533/1‑150 (MQ=60)
                        GGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCAGTTTTTGTACGTGGGTGAA                                                                                                                        >  SRR19973066.1600917/1‑150 (MQ=60)
                        GGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAA                                                                                                                        <  SRR19973066.1784041/150‑1 (MQ=60)
                         GCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAAC                                                                                                                       >  SRR19973066.1359079/1‑150 (MQ=60)
                         GCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAAC                                                                                                                       <  SRR19973066.3266798/150‑1 (MQ=60)
                          CACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACG                                                                                                                      >  SRR19973066.1135034/1‑150 (MQ=60)
                          CACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGGTTGTGAACGTGGGTGAACG                                                                                                                      >  SRR19973066.53543/1‑150 (MQ=60)
                          CACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGGAACATTGGGGAAGATATCCTGTTTGTGAAAGTGGGTGTACG                                                                                                                      >  SRR19973066.1541084/1‑150 (MQ=60)
                           ACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGTACGC                                                                                                                     >  SRR19973066.2883544/1‑150 (MQ=60)
                           ACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGC                                                                                                                     >  SRR19973066.2882586/1‑150 (MQ=60)
                           ACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGC                                                                                                                     >  SRR19973066.2882687/1‑150 (MQ=60)
                             CACGCCACAACATAGTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCAC                                                                                                                   <  SRR19973066.1977893/150‑1 (MQ=60)
                                  CACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTGTGAAAGTGGGTGAACGCACCAACG                                                                                                              >  SRR19973066.2831156/1‑150 (MQ=60)
                                  CACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACG                                                                                                              <  SRR19973066.1642965/150‑1 (MQ=60)
                                   ACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGT                                                                                                             <  SRR19973066.1458271/150‑1 (MQ=60)
                                    CAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTC                                                                                                            <  SRR19973066.3278123/150‑1 (MQ=60)
                                    CAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTC                                                                                                            <  SRR19973066.2282475/150‑1 (MQ=60)
                                      ACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCAC                                                                                                          >  SRR19973066.2325809/1‑150 (MQ=60)
                                       CATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCCGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACC                                                                                                         >  SRR19973066.2991633/1‑150 (MQ=60)
                                         TATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGG                                                                                                       <  SRR19973066.2241932/150‑1 (MQ=60)
                                          ATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCAGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTGAGGCCTGTAGACGCTTAACAATGGATAAGATAGACTGTTTTTGAAAGTGGGTGAACCCACCCACCTCACCCGT                                                                                                      >  SRR19973066.3558591/1‑150 (MQ=60)
                                               AGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGGGAAGCTAGCCTGTTTTTGAACGGGGGTGTACGCACCAACGTCACCGGTTCCGC                                                                                                 >  SRR19973066.3025076/1‑150 (MQ=60)
                                               AGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATATCCTGTTTGTGAACGTGGGTGAACGCACCCACGTCACCCGGTCCGC                                                                                                 >  SRR19973066.3025070/1‑150 (MQ=60)
                                               AGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGC                                                                                                 >  SRR19973066.2083092/1‑150 (MQ=60)
                                               AGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGC                                                                                                 >  SRR19973066.1199136/1‑150 (MQ=60)
                                               AGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATATCCTGTTTGTGTACGTGGGTGAACGCACCCACCGCACCCGTTCCGC                                                                                                 >  SRR19973066.682020/1‑150 (MQ=60)
                                                 CGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTA                                                                                               >  SRR19973066.1929420/1‑150 (MQ=60)
                                                   ATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCGAAGATATCCTGTTTTTGAACGTGGGTGAACGCACCCACCTCACCCGTTCCGCTAAG                                                                                             >  SRR19973066.2184240/1‑150 (MQ=60)
                                                   ATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATATCCTGTTTGTGAACGTGGGTGAACGCCCCAACGTCACCGGTTCCGCTAAG                                                                                             >  SRR19973066.2599597/1‑150 (MQ=60)
                                                   ATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAG                                                                                             >  SRR19973066.2599526/1‑150 (MQ=60)
                                                   ATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAG                                                                                             <  SRR19973066.2919509/150‑1 (MQ=60)
                                                          GTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGGTCCGCTAAGTTCAAGC                                                                                      >  SRR19973066.2010507/1‑150 (MQ=60)
                                                               GTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTG                                                                                 >  SRR19973066.2376618/1‑150 (MQ=60)
                                                               GTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTG                                                                                 >  SRR19973066.2376433/1‑150 (MQ=60)
                                                                 AGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCTGAT                                                                               >  SRR19973066.1052483/1‑150 (MQ=60)
                                                                   AAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCA                                                                             >  SRR19973066.2104455/1‑150 (MQ=60)
                                                                   AAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCA                                                                             >  SRR19973066.1993467/1‑150 (MQ=60)
                                                                    AGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGCACAATTGCTAAGATATCCTGGTTTTGTAAGTGGGGGAACGCACCCACGTCACCCGTTCCGCGAAGTTAAAGCGCCCGATCAA                                                                            >  SRR19973066.2718942/1‑150 (MQ=60)
                                                                     GGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTGTGTCCGGCCTGGAGCCGCTGAACATTGGGTAAGATAGCCGGTTTTTGAACCTGGGTGAACGCACCCACCTCACCGGTTCCGCTAAGTTAAAGCGCCAGATCAAA                                                                           >  SRR19973066.2160274/1‑150 (MQ=60)
                                                                       ATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGGTAAGATTGCCTGTTTTTGTACGTGGGTGAAAGCACCAAAGTCACCGGTTCCGCTAAGTTAAAGCGCCTGATCAAAGA                                                                         >  SRR19973066.357975/1‑150 (MQ=60)
                                                                       ATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCCCCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGA                                                                         >  SRR19973066.2092324/1‑150 (MQ=60)
                                                                         TAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAG                                                                       >  SRR19973066.1492422/1‑150 (MQ=60)
                                                                          AGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAANA                                                                      <  SRR19973066.1359678/150‑1 (MQ=60)
                                                                          AGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGA                                                                      <  SRR19973066.1359774/150‑1 (MQ=60)
                                                                           GCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAG                                                                     <  SRR19973066.1409556/150‑1 (MQ=60)
                                                                              CCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAA                                                                  <  SRR19973066.1558677/150‑1 (MQ=60)
                                                                                 CGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATAC                                                               <  SRR19973066.684491/150‑1 (MQ=60)
                                                                                 CGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATGGCGAAGATAGCCTGTTTGTGTACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATAC                                                               >  SRR19973066.3511133/1‑150 (MQ=60)
                                                                                            GGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCT                                                    <  SRR19973066.3179025/150‑1 (MQ=60)
                                                                                              AAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGG                                                  <  SRR19973066.218708/150‑1 (MQ=60)
                                                                                                ATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTTTGAACGTGGGTGAACGCACCAACCTCACCCGTTACGCTAAGTTAAATCGCCAGATCAAAAAACAGAAAAAAACCGAGGCGCAGGAT                                                >  SRR19973066.1360259/1‑150 (MQ=60)
                                                                                                  TCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGT                                              >  SRR19973066.3569235/1‑150 (MQ=60)
                                                                                                   CCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTC                                             <  SRR19973066.1323326/150‑1 (MQ=60)
                                                                                                   CCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTC                                             <  SRR19973066.1320738/150‑1 (MQ=60)
                                                                                                   CCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTC                                             <  SRR19973066.1225140/150‑1 (MQ=60)
                                                                                                   CCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAAAAATTGGGAACATATCCTTTTTGTGAAAGTTGGTGAACGCACCAAACGCACCCGTTCCGCTAAGTTAAAGCGCCTGATCAAAGAAAAGAAATACAGCGAGGCGCTGGgtttg                                             >  SRR19973066.81646/1‑145 (MQ=60)
                                                                                                   CCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGGAACAATTGCTAAGATATCCGGTTTTTGAACGTGGGTGAAAGCACCAACCTCACCcgttacccgaacttcaagcgcccgttcaaaaaagataaaaaaatcgcggcgcgggttgtg                                             >  SRR19973066.81377/1‑90 (MQ=46)
                                                                                                          CCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTC                                      <  SRR19973066.2682476/150‑1 (MQ=60)
                                                                                                          CCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACAATGGCGAAGATATCCTGTTTGTTAACGTGGGGGAACGCACCAACCTCACCCGTTCCGCGAAGTTCAAACCCCGGATAAAATAAGAGAAAAAAACCGAGGCGCTGGATGTCGCGCGTC                                      >  SRR19973066.141640/1‑150 (MQ=60)
                                                                                                           CTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCA                                     <  SRR19973066.2278100/150‑1 (MQ=60)
                                                                                                           CTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCA                                     <  SRR19973066.1210115/150‑1 (MQ=60)
                                                                                                           CTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCA                                     <  SRR19973066.2902370/150‑1 (MQ=60)
                                                                                                           CTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCA                                     <  SRR19973066.2902388/150‑1 (MQ=60)
                                                                                                            TGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTTGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACCTCACCGGTTACGCTAATTTCAAGCGCCTGAAAAAAGAAAAGAAATAAAGCGAGGCGCTGGATGTCGCGCGTCAA                                    >  SRR19973066.1570048/1‑150 (MQ=60)
                                                                                                            TGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTTGGTGAACGCACCAACCTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGATAAATACAGCGAGGCGCTGGATGTCGCGCGTCAA                                    >  SRR19973066.3387349/1‑150 (MQ=60)
                                                                                                            TGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCCCCCGTTCCGCGAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACATCGAGGCGCTGGATGTCGCGCGTCAA                                    >  SRR19973066.3387225/1‑150 (MQ=60)
                                                                                                             GCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAAC                                   <  SRR19973066.682653/150‑1 (MQ=60)
                                                                                                               CGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAG                                 <  SRR19973066.1131452/150‑1 (MQ=60)
                                                                                                               CGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAG                                 <  SRR19973066.2645916/150‑1 (MQ=60)
                                                                                                                GTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCGGGATGTCGCGCGTCAACAGG                                >  SRR19973066.3433065/1‑150 (MQ=60)
                                                                                                                 TTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGT                               <  SRR19973066.1519298/150‑1 (MQ=60)
                                                                                                                   TGTCCGGCCTGGAGGCGCTGATCATTGTCTAAGATAGCCTGTTTGTGACCGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGG                             <  SRR19973066.1606660/150‑1 (MQ=60)
                                                                                                                   TGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGG                             <  SRR19973066.1606277/150‑1 (MQ=60)
                                                                                                                          CCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCNAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGG                      >  SRR19973066.1822876/1‑150 (MQ=60)
                                                                                                                            TGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCG                    <  SRR19973066.282689/150‑1 (MQ=60)
                                                                                                                            TGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCG                    <  SRR19973066.282688/150‑1 (MQ=60)
                                                                                                                            TGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCG                    <  SRR19973066.281680/150‑1 (MQ=60)
                                                                                                                            TGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCG                    <  SRR19973066.3432857/150‑1 (MQ=60)
                                                                                                                            TGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCG                    >  SRR19973066.2469948/1‑150 (MQ=60)
                                                                                                                            TGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCG                    >  SRR19973066.668468/1‑150 (MQ=60)
                                                                                                                            TGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCG                    >  SRR19973066.668447/1‑150 (MQ=60)
                                                                                                                                   GCTGAACTTTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGAT             <  SRR19973066.2430508/150‑1 (MQ=60)
                                                                                                                                   GCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGAT             <  SRR19973066.2430488/150‑1 (MQ=60)
                                                                                                                                   GCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGAT             >  SRR19973066.1616050/1‑150 (MQ=60)
                                                                                                                                   GCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACAAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGAT             <  SRR19973066.3211022/150‑1 (MQ=60)
                                                                                                                                    CTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATT            >  SRR19973066.1201487/1‑150 (MQ=60)
                                                                                                                                      GAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTAT          >  SRR19973066.888280/1‑150 (MQ=60)
                                                                                                                                       AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATC         >  SRR19973066.1631446/1‑150 (MQ=60)
                                                                                                                                       AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATC         >  SRR19973066.1631433/1‑150 (MQ=60)
                                                                                                                                       AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATC         >  SRR19973066.1630267/1‑150 (MQ=60)
                                                                                                                                          ATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT      >  SRR19973066.923334/1‑150 (MQ=60)
                                                                                                                                          ATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT      >  SRR19973066.3216271/1‑150 (MQ=60)
                                                                                                                                          ATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT      >  SRR19973066.3215633/1‑150 (MQ=60)
                                                                                                                                              GCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCA  >  SRR19973066.3061836/1‑150 (MQ=60)
                                                                                                                                                |                                                                                                                                                   
CTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATCA  >  CP009273/4214665‑4214956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑