Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F434 I0 R1	1130	215.9	7031662	97.1%	6827743	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,214,809	G→T	100%	E352* (GAA→TAA)	metH →	homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,214,809	0	G	T	95.6%	577.8 / 5.7	221	E352* (GAA→TAA)	metH	homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand): ref base G (12/2); new base T (122/85); total (134/87)
Fisher's exact test for biased strand distribution p-value = 5.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 221 total aligned reads displayed.

TCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATA  >  CP009273/4214664‑4214953

 ctcAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAg                                                                                                                                               >  1:2480153/1‑148 (MQ=255)

 ctcAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAg                                                                                                                                               >  1:2927766/1‑148 (MQ=255)

   cAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGAt                                                                                                                                             <  2:1594675/148‑1 (MQ=255)

    aaTATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGaaa                                                                                                                                            >  1:2430776/1‑146 (MQ=255)

    aaTATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATa                                                                                                                                            >  2:3157706/1‑148 (MQ=255)

     atatCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAg                                                                                                                                           <  1:1658261/148‑1 (MQ=255)

          gtcgGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGt                                                                                                                                      <  1:3191059/148‑1 (MQ=255)

          gtcgGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGt                                                                                                                                      <  1:3413957/148‑1 (MQ=255)

                  ctgctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAAc                                                                                                                              >  2:3048718/1‑148 (MQ=255)

                  ctgctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAAc                                                                                                                              >  2:1848596/1‑148 (MQ=255)

                  ctgctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAAc                                                                                                                              >  1:356936/1‑148 (MQ=255)

                  ctgctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑AACATTGGGGAAGATAGCCTGTTTGTGAAc                                                                                                                              >  1:2655940/1‑148 (MQ=255)

                    gctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGt                                                                                                                            <  1:1039365/148‑1 (MQ=255)

                    gctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGGTAATATAGCCTGTTTTTGaaact                                                                                                                            >  1:783950/1‑145 (MQ=255)

                     ctgTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTg                                                                                                                           >  2:3327551/1‑148 (MQ=255)

                       gtgGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTggg                                                                                                                         <  2:2813850/148‑1 (MQ=255)

                        tgGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGt                                                                                                                        >  2:2234757/1‑148 (MQ=255)

                           caccacGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGaa                                                                                                                     <  2:1006710/148‑1 (MQ=255)

                           caccacGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGaa                                                                                                                     <  1:950035/148‑1 (MQ=255)

                             ccacGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACg                                                                                                                   >  2:2778857/1‑148 (MQ=255)

                               acGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCa                                                                                                                 <  2:2572001/148‑1 (MQ=255)

                                 gCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCAcc                                                                                                               >  1:3437118/1‑148 (MQ=255)

                                    acaacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAAc                                                                                                            >  1:3094563/1‑148 (MQ=255)

                                    acaacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑GACAATGGCGAATATAGCCAGTTTTTGAAAGTGGGGGAACGCACCCAc                                                                                                            >  1:1200988/1‑148 (MQ=255)

                                     caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACg                                                                                                           >  1:521368/1‑148 (MQ=255)

                                     caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACg                                                                                                           >  1:1455975/1‑148 (MQ=255)

                                     caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACg                                                                                                           >  1:2933015/1‑148 (MQ=255)

                                     caacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGGGGGTGAACGCACCAACg                                                                                                           >  1:521122/1‑148 (MQ=255)

                                        caTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCa                                                                                                        >  1:424491/1‑148 (MQ=255)

                                         atatTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGTACGCACCAACGTCAc                                                                                                       >  1:1025424/1‑148 (MQ=255)

                                          tatTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCAcc                                                                                                      >  2:2901034/1‑148 (MQ=255)

                                          tatTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCTGTTTGTGAACGTGGGTGTAAGCACCAACGTCAcc                                                                                                      >  1:2985517/1‑148 (MQ=255)

                                           atTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCg                                                                                                     <  1:2638082/148‑1 (MQ=255)

                                           atTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCg                                                                                                     >  1:2794343/1‑148 (MQ=255)

                                             tGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGt                                                                                                   >  1:2043635/1‑148 (MQ=255)

                                             tGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGt                                                                                                   <  2:1076000/148‑1 (MQ=255)

                                             tGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGt                                                                                                   >  2:2009252/1‑148 (MQ=255)

                                              gCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGtt                                                                                                  <  1:2315563/148‑1 (MQ=255)

                                               cAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTc                                                                                                 >  2:1196068/1‑148 (MQ=255)

                                               cAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTc                                                                                                 >  2:913888/1‑148 (MQ=255)

                                               cAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTc                                                                                                 >  1:1669080/1‑148 (MQ=255)

                                                  cGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATATCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGc                                                                                              >  1:3002159/1‑148 (MQ=255)

                                                    ctGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTa                                                                                            <  2:2522151/147‑1 (MQ=255)

                                                    aTGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCACACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTa                                                                                            <  2:3383551/148‑1 (MQ=255)

                                                    aTGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTa                                                                                            >  2:441983/1‑148 (MQ=255)

                                                    aTGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCCGCCTGGAGCCGCGG‑AAAAATGGCTAATATATCCTGTTTTTGAACGTGGGTGTAAGCACCAACCTCACCGGTTccccga                                                                                            >  1:1249551/1‑144 (MQ=255)

                                                      gAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAAACATT‑GCGAAGATAACCATTTTTTGAACCTGGGGGAACCCACCAACGTCACCCGTTACGCTaat                                                                                          >  1:1556535/1‑147 (MQ=255)

                                                      gAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCTGTTTTTGAACGTGGGTGAACGCACCAACCTCACCGGTTCCGCGAAg                                                                                          >  1:1382978/1‑148 (MQ=255)

                                                               aGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgcgc                                                                                 <  1:1934938/148‑1 (MQ=255)

                                                                 tAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCt                                                                               >  1:2780546/1‑148 (MQ=255)

                                                                  aGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTg                                                                              <  2:3289618/148‑1 (MQ=255)

                                                                   gAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGa                                                                             <  1:2347595/148‑1 (MQ=255)

                                                                    aaGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGAt                                                                            <  1:2161125/148‑1 (MQ=255)

                                                                      ggATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCa                                                                          >  2:1252731/1‑148 (MQ=255)

                                                                       gATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGGTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCaa                                                                         >  1:1585865/1‑148 (MQ=255)

                                                                       gATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCaa                                                                         >  1:2605305/1‑148 (MQ=255)

                                                                         ttAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCTGATCAaag                                                                       >  2:2665766/1‑148 (MQ=255)

                                                                          tAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaaga                                                                      <  2:1339325/148‑1 (MQ=255)

                                                                          tAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaaga                                                                      <  2:1338733/148‑1 (MQ=255)

                                                                            gcgcCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaagaag                                                                    <  2:809311/148‑1 (MQ=255)

                                                                            gcgcCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCGGATCAaagaag                                                                    >  1:1589276/1‑148 (MQ=255)

                                                                               ccgcGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAgaga                                                                 <  2:763983/148‑1 (MQ=255)

                                                                               ccgcGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAgaga                                                                 <  2:1699543/148‑1 (MQ=255)

                                                                                cgcgCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGaa                                                                <  1:2028318/148‑1 (MQ=255)

                                                                                 gcgcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGaaa                                                               <  1:436305/148‑1 (MQ=255)

                                                                                   gcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATa                                                             <  2:545681/148‑1 (MQ=255)

                                                                                   gcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATa                                                             <  2:356936/148‑1 (MQ=255)

                                                                                     aaaCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACa                                                           <  1:1013029/148‑1 (MQ=255)

                                                                                     aaaCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACa                                                           <  2:2082067/148‑1 (MQ=255)

                                                                                      aaCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCTAAGATATCCTGTTTGTGAACGGGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAg                                                          >  1:1962535/1‑148 (MQ=255)

                                                                                      aaCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAg                                                          >  2:2904344/1‑148 (MQ=255)

                                                                                       aCTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTTGTTGTGAACGTGGGTGTACGCACCAACGTCACCGGTTCCGCTAAGTTCAATCGCGTGATCAAAGTAGAGAAATACAGc                                                         >  2:590470/1‑148 (MQ=255)

                                                                                        cTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTT‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCg                                                        <  1:3102685/148‑1 (MQ=255)

                                                                                        cTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCg                                                        <  1:1850753/148‑1 (MQ=255)

                                                                                              gAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGgcgc                                                  >  2:1248514/1‑148 (MQ=255)

                                                                                                 aTTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTgg                                               <  2:859826/148‑1 (MQ=255)

                                                                                                 aTTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCTGATCAAAGAAGAGAAATAAAGCGAGGCGCTgg                                               >  2:1509177/1‑148 (MQ=255)

                                                                                                  ttCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCTAAGATAGCCAGTTTTTGAAAGTGGGTGAAAGCACCCACGTCACCCGTTGCGCGAATGTAAAGCGCCAGGTCAAACAAGAGTAAAACACCGAGGCGCTGGa                                              >  1:2413949/1‑148 (MQ=255)

                                                                                                  ttCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGa                                              >  1:3194460/1‑148 (MQ=255)

                                                                                                  ttCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGa                                              >  1:1448928/1‑148 (MQ=255)

                                                                                                  ttCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AAAATTTGCTAAGATAGCCTGTTTTTGTACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAATCGCCGGATCAAATAAGAGAAAAACAGCGAGGCGCGGGa                                              >  1:1363832/1‑148 (MQ=255)

                                                                                                   tCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCGGTTTGTTAAACTGGGTGAACGCCCCCACCTCCCCCGTTCCGCGAAGATAAAGCGCCCGATAAAATAAGAGAAAAAAACAGAGGCGCTGGAt                                             >  1:1947530/1‑148 (MQ=255)

                                                                                                    cccGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATg                                            <  1:256615/148‑1 (MQ=255)

                                                                                                      cGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTc                                          >  2:2875562/1‑148 (MQ=255)

                                                                                                      cGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTc                                          >  2:3121675/1‑148 (MQ=255)

                                                                                                      cGTAGCCTGCCGTTTGTCCGGCCATGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTTTGAACGTGGGTGAACGCACCAACGTCACCGGTTACGCTAAGTTAAAGCCCCTGATAAAAGAAGAGAAATAAAGCGAGGCGCTGGATGTc                                          >  2:58441/1‑148 (MQ=255)

                                                                                                           ccTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTcgcgcg                                     >  2:2175022/1‑148 (MQ=255)

                                                                                                                cGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAAc                                >  2:1100434/1‑148 (MQ=255)

                                                                                                                 gTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACa                               <  2:2480153/148‑1 (MQ=255)

                                                                                                                 gTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGAGAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACa                               <  2:476024/148‑1 (MQ=255)

                                                                                                                       ccGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTAAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGa                         >  1:724262/1‑148 (MQ=255)

                                                                                                                             tGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACgg                   >  2:2339936/1‑148 (MQ=255)

                                                                                                                                 gCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGTTGTCGCGCGTCAACAGGTGGAAAACGgcgcg               >  2:587498/1‑148 (MQ=255)

                                                                                                                                 gCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgcg               >  2:3076884/1‑148 (MQ=255)

                                                                                                                                  ccGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgcgc              >  1:85061/1‑148 (MQ=255)

                                                                                                                                     cTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGa           >  2:3433515/1‑148 (MQ=255)

                                                                                                                                     cTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGa           <  1:1708017/148‑1 (MQ=255)

                                                                                                                                       g‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGAtt         >  1:1150409/1‑148 (MQ=255)

                                                                                                                                         aaCATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTa        <  2:1937214/148‑1 (MQ=255)

                                                                                                                                              tGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGat   >  1:1873378/1‑148 (MQ=255)

TCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATA  >  CP009273/4214664‑4214953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATC  >  CP009273/4214661‑4214955

TTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTA                                                                                                                                                   <  SRR19973065.3502930/150‑1 (MQ=60)

  TTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCTAAG                                                                                                                                                 >  SRR19973065.1565684/1‑150 (MQ=60)

  TTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAG                                                                                                                                                 >  SRR19973065.2945542/1‑150 (MQ=60)

  TTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAG                                                                                                                                                 >  SRR19973065.2495767/1‑150 (MQ=60)

  TTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAG                                                                                                                                                 >  SRR19973065.2495738/1‑150 (MQ=60)

     TCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCCCGG‑AACATTGGCTAAGAAA                                                                                                                                              >  SRR19973065.2446114/1‑150 (MQ=60)

        ATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCC                                                                                                                                           <  SRR19973065.1669369/150‑1 (MQ=60)

        ATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑AACATTTGCGAAGATAGCC                                                                                                                                           >  SRR19973065.313959/1‑150 (MQ=60)

         TATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGAAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCGAAGATAGCCT                                                                                                                                          >  SRR19973065.1578498/1‑150 (MQ=60)

          ATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTG                                                                                                                                         <  SRR19973065.2327366/150‑1 (MQ=60)

             GTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTT                                                                                                                                      <  SRR19973065.1685848/150‑1 (MQ=60)

             GTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTT                                                                                                                                      <  SRR19973065.3434843/150‑1 (MQ=60)

             GTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTT                                                                                                                                      <  SRR19973065.3434820/150‑1 (MQ=60)

             GTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTT                                                                                                                                      <  SRR19973065.3210306/150‑1 (MQ=60)

             GTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTT                                                                                                                                      <  SRR19973065.3211095/150‑1 (MQ=60)

              TCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTG                                                                                                                                     <  SRR19973065.3015847/150‑1 (MQ=60)

                GGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTG                                                                                                                                   <  SRR19973065.3229432/150‑1 (MQ=60)

                   GGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCTAAGATAGCCTGTTTTTGAAC                                                                                                                                >  SRR19973065.359449/1‑150 (MQ=60)

                   GGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑AACATTGGGGAAGATAGCCTGTTTGTGAAC                                                                                                                                >  SRR19973065.2672355/1‑150 (MQ=60)

                       GCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCTAAGATAGCCTGTTTGTGAACGTGG                                                                                                                            >  SRR19973065.2381468/1‑150 (MQ=60)

                       GCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGG                                                                                                                            <  SRR19973065.1046340/150‑1 (MQ=60)

                       GCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGG                                                                                                                            <  SRR19973065.1046244/150‑1 (MQ=60)

                              CACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACG                                                                                                                     <  SRR19973065.956312/150‑1 (MQ=60)

                               ACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGC                                                                                                                    >  SRR19973065.2791796/1‑150 (MQ=60)

                                  ACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACC                                                                                                                 >  SRR19973065.3458086/1‑150 (MQ=60)

                                   CGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCA                                                                                                                <  SRR19973065.1876316/150‑1 (MQ=60)

                                     CCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAAC                                                                                                              >  SRR19973065.3113455/1‑150 (MQ=60)

                                     CCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑GACAATGGCGAATATAGCCAGTTTTTGAAAGTGGGGGAACGCACCCAC                                                                                                              >  SRR19973065.1208667/1‑150 (MQ=60)

                                      CACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACG                                                                                                             >  SRR19973065.1464985/1‑150 (MQ=60)

                                      CACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACG                                                                                                             >  SRR19973065.524802/1‑150 (MQ=60)

                                      CACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACG                                                                                                             >  SRR19973065.2950805/1‑150 (MQ=60)

                                      CACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACG                                                                                                             >  SRR19973065.1465351/1‑150 (MQ=60)

                                      CACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGGGGGTGAACGCACCAACG                                                                                                             >  SRR19973065.524556/1‑150 (MQ=60)

                                      CACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGGTTGTGAACGTGGGTGAACGCACCAACG                                                                                                             >  SRR19973065.524601/1‑150 (MQ=60)

                                       ACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGT                                                                                                            >  SRR19973065.793769/1‑150 (MQ=60)

                                         AACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCA                                                                                                          >  SRR19973065.427446/1‑150 (MQ=60)

                                         AACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATTGGTAAGATAGACTGGTTGTGAACGTGGGTGAACGCACCAACGTCA                                                                                                          >  SRR19973065.429708/1‑150 (MQ=60)

                                          ACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTTTGAACGTGGGTGGACGCACCCACGTCAC                                                                                                         >  SRR19973065.1032227/1‑150 (MQ=60)

                                          ACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGTACGCACCAACGTCAC                                                                                                         >  SRR19973065.1032210/1‑150 (MQ=60)

                                          ACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCAC                                                                                                         >  SRR19973065.1032205/1‑150 (MQ=60)

                                           CATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCGAAGTTAGCCTGTTTGTTTACGTGGGGGTACGCCCCAACGTCACC                                                                                                        >  SRR19973065.1246094/1‑150 (MQ=60)

                                           CATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATATCCTGTTTGTGAACCTGGGTGAACGCACCAACCTCACC                                                                                                        >  SRR19973065.3003775/1‑150 (MQ=60)

                                           CATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCTGTTTGTGAACGTGGGTGTAAGCACCAACGTCACC                                                                                                        >  SRR19973065.3003792/1‑150 (MQ=60)

                                            ATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCCACGTCACCG                                                                                                       >  SRR19973065.2811657/1‑150 (MQ=60)

                                              ATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGT                                                                                                     <  SRR19973065.2654374/150‑1 (MQ=60)

                                              ATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGT                                                                                                     >  SRR19973065.2056714/1‑150 (MQ=60)

                                                TGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTC                                                                                                   >  SRR19973065.1680247/1‑150 (MQ=60)

                                                 GCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCC                                                                                                  <  SRR19973065.2330086/150‑1 (MQ=60)

                                                 GCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCC                                                                                                  <  SRR19973065.2330088/150‑1 (MQ=60)

                                                   AGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATATCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGC                                                                                                >  SRR19973065.3020552/1‑150 (MQ=60)

                                                   AGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCCACCTCACCGGTTCCGC                                                                                                >  SRR19973065.3020556/1‑150 (MQ=60)

                                                     CGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCTAAGAAAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCCGTTCCGCTA                                                                                              >  SRR19973065.1395768/1‑150 (MQ=60)

                                                     CGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTA                                                                                              <  SRR19973065.3500060/150‑1 (MQ=60)

                                                     CGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCTGTTTGTGAACGTGGGGGAACGCACCAACGTCACCGGTTCCGCGA                                                                                              >  SRR19973065.361718/1‑150 (MQ=60)

                                                     CGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCCGCCTGGAGCCGCGG‑AAAAATGGCTAATATATCCTGTTTTTGAACGTGGGTGTAAGCACCAACCTCACCGGTTCCccga                                                                                              >  SRR19973065.1257779/1‑146 (MQ=60)

                                                       ATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAAACATT‑GCGAAGATAACCATTTTTTGAACCTGGGGGAACCCACCAACGTCACCCGTTACGCTAAT                                                                                            >  SRR19973065.1566861/1‑150 (MQ=60)

                                                       ATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATTGCTAAGATAGCCTGTTTTTGAAAGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAC                                                                                            >  SRR19973065.1391973/1‑150 (MQ=60)

                                                       ATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCTGTTTTTGAACGTGGGTGAACGCACCAACCTCACCGGTTCCGCGAAG                                                                                            >  SRR19973065.1392080/1‑150 (MQ=60)

                                                               TGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCG                                                                                    <  SRR19973065.2663961/150‑1 (MQ=60)

                                                                  AGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCTAAGATAGCCTGTTTGTGAAAGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCT                                                                                 >  SRR19973065.2798235/1‑150 (MQ=60)

                                                                  AGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCT                                                                                 >  SRR19973065.2798245/1‑150 (MQ=60)

                                                                  AGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCT                                                                                 >  SRR19973065.2797799/1‑150 (MQ=60)

                                                                  AGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCT                                                                                 <  SRR19973065.1947603/150‑1 (MQ=60)

                                                                    TAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑TACATTGGCTAAGATAtgcggtttttgtaagggggtgtacgcaccaacgtcacccggtgcgcgaagttaaaccgccaga                                                                               >  SRR19973065.3264043/1‑87 (MQ=60)

                                                                      GAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATC                                                                             <  SRR19973065.2362227/150‑1 (MQ=60)

                                                                       AAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCA                                                                            <  SRR19973065.2175019/150‑1 (MQ=60)

                                                                        AGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGGTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAA                                                                           >  SRR19973065.1596441/1‑150 (MQ=60)

                                                                        AGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGGGAAGATATCCGGTTTGTGAACGTGGGTGCACGCACCAACGTCCCCGGGTGCGCTAAGTTCAAtcgccggataca                                                                           >  SRR19973065.1597229/1‑138 (MQ=60)

                                                                        AGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTAAAGCGCCTGATCAA                                                                           >  SRR19973065.2621475/1‑150 (MQ=60)

                                                                        AGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTGTGTCCGGCCTGGAGCCGCGG‑AACCATGGCGAAGATAtcccggttttgtacggggggggacgcccccaccgcacccggtgcgcgcacttgaaccgccgggtcca                                                                           >  SRR19973065.612199/1‑83 (MQ=60)

                                                                        AGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTGTGTCCGGCCGGGAGCCGCGG‑AACCGTGGGGAAGATAGCCGGTTTTTGTACGGGGGGGTACGCACCCACCTCACCCGGTCCGCGAAGTTGAAGCGCCGGATCAA                                                                           >  SRR19973065.612273/1‑150 (MQ=47)

                                                                             TAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAG                                                                      >  SRR19973065.1678743/1‑150 (MQ=60)

                                                                             TAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCGAAGATAGCCGGTTTGTGTACGTGGGTGTACGCACCAACGTCACCGGGTCCGCTAAGTTCAAGCGCCGGATCAAAGAAG                                                                      >  SRR19973065.1600133/1‑150 (MQ=60)

                                                                             TAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑AACATTTGGTAAGATAGCCTGTTTGTGTAAGTGGGTGTACGCACCAACGgcacacggtcgcctaagttaaaccgccggatcaaacaac                                                                      >  SRR19973065.3350889/1‑111 (MQ=60)

                                                                             TAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCGG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCGGATCAAAGAAG                                                                      >  SRR19973065.1599885/1‑150 (MQ=60)

                                                                              AGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTGTGTCCGGCCGGGGGCCGCGG‑GAAATTGGCGAAGATAGgcagttttttgacgtgggtggacgcacccaacggcccggttgcgcgtagttcaagcgccgggtcaaaaaaga                                                                     >  SRR19973065.302366/1‑78 (MQ=60)

                                                                               GCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAG                                                                    <  SRR19973065.1693136/150‑1 (MQ=60)

                                                                                CGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGGGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCTGATCAAAGAAGAGA                                                                   >  SRR19973065.2047408/1‑150 (MQ=60)

                                                                                   CGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAAT                                                                <  SRR19973065.2041346/150‑1 (MQ=60)

                                                                                   CGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAAT                                                                >  SRR19973065.1166004/1‑150 (MQ=60)

                                                                                    GCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATA                                                               <  SRR19973065.439333/150‑1 (MQ=60)

                                                                                      GCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCGAAGATATCCTGTTTTTGAACGTGGGTGAACGCACCAACCTCACCGGTTGCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACA                                                             >  SRR19973065.2432377/1‑150 (MQ=60)

                                                                                       CAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCTAAGATATCCTGTTTGTGAACGGGGGTGAACGCACCAACGTCACCGGTTCCGCGAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAG                                                            >  SRR19973065.1975349/1‑150 (MQ=60)

                                                                                        AAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGC                                                           <  SRR19973065.1019744/150‑1 (MQ=60)

                                                                                         AACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCG                                                          <  SRR19973065.2593463/150‑1 (MQ=60)

                                                                                         AACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AAAATTTGGTAAGATAGCCGGTTTTTGAACGGGGGGGTAAGCACCAACGTCACCGGTTCCGCGAATTTAAAGCGCCGGATCAAAGAATAGAAAAACAGCC                                                          >  SRR19973065.1251545/1‑150 (MQ=60)

                                                                                           CTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTT‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAG                                                        <  SRR19973065.3121616/150‑1 (MQ=60)

                                                                                           CTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAG                                                        <  SRR19973065.1363760/150‑1 (MQ=60)

                                                                                           CTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAG                                                        <  SRR19973065.1862947/150‑1 (MQ=60)

                                                                                           CTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAG                                                        <  SRR19973065.1862899/150‑1 (MQ=60)

                                                                                           CTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAG                                                        <  SRR19973065.3121600/150‑1 (MQ=60)

                                                                                           CTGCCGGAAATTCCCGTAGCCTGCCGTTTGTACCGCCAGGAGCCGCTG‑AACAATTGGGAAGATAACCTGTTTTTGAACGTGGGTGAACGCACCAACGTCACCCGTTACGCTAACTTAAATCGCCTGATCAAAGAAGAcacaaaaaacgag                                                        >  SRR19973065.2536299/1‑137 (MQ=60)

                                                                                                   AATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCTAAGATAGCCAGTTTTTGAAAGTGGGTGAAAGCACCCACGTCACCCGTTGCGCGAATGTAAAGCGCCAGGTCAAACAAGAGTAAAACACCGAGGCGCTGGA                                                >  SRR19973065.2429156/1‑150 (MQ=51)

                                                                                                   AATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTTTGAACGTGGGTGAACGCACCAACGTCACCCGTTACGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAAAACAGCGAGGCGCTGGA                                                >  SRR19973065.1972524/1‑150 (MQ=60)

                                                                                                   AATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGTACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGA                                                >  SRR19973065.1972527/1‑150 (MQ=60)

                                                                                                   AATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGA                                                >  SRR19973065.3213718/1‑150 (MQ=60)

                                                                                                   AATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGTAGAGAAATACAGCGAGGCGCTGGA                                                >  SRR19973065.1458285/1‑150 (MQ=60)

                                                                                                   AATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCGAAGATATCCTGTTTGTGAACGTGGGTGAACGCACCAACCTCACCGGTTCCGCTAAGTTCAAGCGCCAGATCAAATAAGATAAATAAAGCGAGGCGCTGGA                                                >  SRR19973065.1371163/1‑150 (MQ=60)

                                                                                                   AATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AAAATTTGCTAAGATAGCCTGTTTTTGTACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAATCGCCGGATCAAATAAGAGAAAAACAGCGAGGCGCGGGA                                                >  SRR19973065.1372854/1‑150 (MQ=60)

                                                                                                    ATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACAATGGCTAAGATAGCCGGTTTGTTAAACTGGGTGAACGCccccacctcccccgttccgcgaagataaagcgcccgataaaataagagaaaaaaacagaggcgctggat                                               >  SRR19973065.1960258/1‑81 (MQ=60)

                                                                                                       CCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTTGCGAATATATCCAGTTTTTTAAAGTGGGTGAACGCACCAACGTCACCCGTTCCCCTAAATTAAATAGCCAGATAAAATAAAAGAAAAAAAGCGAGGCGCTGGATGTC                                            >  SRR19973065.780154/1‑150 (MQ=47)

                                                                                                       CCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTC                                            <  SRR19973065.258401/150‑1 (MQ=60)

                                                                                                       CCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTC                                            <  SRR19973065.258387/150‑1 (MQ=60)

                                                                                                            AGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAAAGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAATCGCCAGATCAAAGAAGATAAATACAGCGAGGCGCTGGATGTCGCGCG                                       >  SRR19973065.2622649/1‑150 (MQ=60)

                                                                                                              CCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AAAATTTGCTAAGATAGCCTTTTTTTGAAAGTGGGTGAACGCAACAACGTCACCCGTTACCCTAAATTAAAACCCCGGATAAAAAAAAAGAAAAAAACCGAGGCGCTGGATGTCGCGCGTC                                     >  SRR19973065.2169220/1‑150 (MQ=35)

                                                                                                               CTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCA                                    <  SRR19973065.872190/150‑1 (MQ=60)

                                                                                                                TGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAA                                   >  SRR19973065.3057006/1‑150 (MQ=60)

                                                                                                                        GTCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTAAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGA                           >  SRR19973065.729111/1‑150 (MQ=60)

                                                                                                                         TCCGGCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAA                          <  SRR19973065.2950661/150‑1 (MQ=60)

                                                                                                                             GCCTGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACG                      <  SRR19973065.2818439/150‑1 (MQ=60)

                                                                                                                                TGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCG                   <  SRR19973065.893992/150‑1 (MQ=60)

                                                                                                                                TGGAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCG                   <  SRR19973065.2027675/150‑1 (MQ=60)

                                                                                                                                  GAGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCG                 >  SRR19973065.2192042/1‑150 (MQ=60)

                                                                                                                                   AGCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGC                >  SRR19973065.85888/1‑150 (MQ=60)

                                                                                                                                    GCCGCTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCA               <  SRR19973065.2511273/150‑1 (MQ=60)

                                                                                                                                        CTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATT           <  SRR19973065.1719379/150‑1 (MQ=60)

                                                                                                                                        CTG‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATT           >  SRR19973065.1157821/1‑150 (MQ=60)

                                                                                                                                          G‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTAT         <  SRR19973065.462328/150‑1 (MQ=60)

                                                                                                                                          G‑AACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTAT         <  SRR19973065.462358/150‑1 (MQ=60)

                                                                                                                                               ATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT     >  SRR19973065.98097/1‑150 (MQ=60)

                                                                                                                                               ATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT     <  SRR19973065.1476357/150‑1 (MQ=60)

                                                                                                                                               ATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGAT     >  SRR19973065.1885714/1‑150 (MQ=60)

                                                                                                                                                  GGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATC  <  SRR19973065.1945382/150‑1 (MQ=60)

                                                                                                                                                  GGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATC  <  SRR19973065.1948124/150‑1 (MQ=60)

TTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTG‑AACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGATTATCGATATC  >  CP009273/4214661‑4214955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`