Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F181 I0 R1	1262	323.1	10297870	97.5%	10040423	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,217,818	G→T	8.2%	V54F (GTT→TTT)	yjbB →	putative Na+/Pi‑cotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,217,818	0	G	T	8.2%	1264.1 / 55.1	335	V54F (GTT→TTT)	yjbB	putative Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand): ref base G (148/159); new base T (17/11); total (165/170)
Fisher's exact test for biased strand distribution p-value = 2.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 344 total aligned reads displayed.

TGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATGGCGCGTATTCTCACCTTCGAC  >  CP009273/4217669‑4217967

   ttCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTa                                                                                                                                                    <  1:5074150/150‑1 (MQ=255)

     cACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTAcc                                                                                                                                                  <  2:1237177/150‑1 (MQ=255)

      aCCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCg                                                                                                                                                 >  2:1926842/1‑150 (MQ=255)

          gcgttCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTTTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACt                                                                                                                                             <  2:5129633/147‑1 (MQ=255)

          gCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACt                                                                                                                                             >  2:625016/1‑150 (MQ=255)

            tttCTGCCGTCGCCCAGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGATTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTgg                                                                                                                                           >  1:1701897/1‑150 (MQ=255)

             ttCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGt                                                                                                                                          <  2:2598467/150‑1 (MQ=255)

                  ccGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACaga                                                                                                                                     <  2:550853/150‑1 (MQ=255)

                   cGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACagag                                                                                                                                    <  1:4676385/150‑1 (MQ=255)

                    gTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGc                                                                                                                                   >  2:693383/1‑150 (MQ=255)

                    gTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGc                                                                                                                                   >  2:4239081/1‑150 (MQ=255)

                     tCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCTTTACCGCACTGGTACAGAGCa                                                                                                                                  >  1:2061995/1‑150 (MQ=255)

                     tCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCa                                                                                                                                  <  1:591218/150‑1 (MQ=255)

                      cGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCG‑AAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGt                                                                                                                                <  2:3914628/150‑1 (MQ=255)

                       gCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGt                                                                                                                                <  2:1657916/150‑1 (MQ=255)

                       gCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGt                                                                                                                                <  2:4220084/150‑1 (MQ=255)

                        cccTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTa                                                                                                                               <  2:5133203/150‑1 (MQ=255)

                        cccTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTa                                                                                                                               <  1:2414064/150‑1 (MQ=255)

                         ccTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTaa                                                                                                                              <  1:1173395/150‑1 (MQ=255)

                                 tCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGccacca                                                                                                                      <  2:2669055/150‑1 (MQ=255)

                                  cTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGccaccac                                                                                                                     >  2:2338185/1‑150 (MQ=255)

                                   tGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGccaccacc                                                                                                                    <  2:324399/150‑1 (MQ=255)

                                    gggggACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGccaccacca                                                                                                                   >  2:4454805/1‑150 (MQ=255)

                                     ggggACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCAt                                                                                                                  >  1:747984/1‑150 (MQ=255)

                                        gACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCt                                                                                                               >  2:1474419/1‑150 (MQ=255)

                                          cTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTgg                                                                                                             >  2:3353405/1‑150 (MQ=255)

                                               atTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGAcc                                                                                                        >  2:5079647/1‑150 (MQ=255)

                                                 tGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTc                                                                                                      >  2:105346/1‑150 (MQ=255)

                                                  gTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACGGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCg                                                                                                     <  1:799279/150‑1 (MQ=255)

                                                   ttCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCTTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGt                                                                                                    >  1:2289732/1‑150 (MQ=255)

                                                     cGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGttt                                                                                                  <  1:1562701/150‑1 (MQ=255)

                                                      gAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTg                                                                                                 >  1:2250938/1‑150 (MQ=255)

                                                        aCCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTc                                                                                               >  1:2895708/1‑150 (MQ=255)

                                                              gTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAg                                                                                         >  2:4673704/1‑150 (MQ=255)

                                                               tAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAgg                                                                                        >  2:3778997/1‑150 (MQ=255)

                                                               tAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAgg                                                                                        >  1:3451130/1‑150 (MQ=255)

                                                               tAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAgg                                                                                        <  1:4520199/150‑1 (MQ=255)

                                                                aaTGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCTTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGa                                                                                       >  1:2465895/1‑150 (MQ=255)

                                                                  tGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATc                                                                                     <  1:4275435/150‑1 (MQ=255)

                                                                  tGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATc                                                                                     >  1:3134976/1‑150 (MQ=255)

                                                                  tGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCGTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATc                                                                                     >  2:1268255/1‑150 (MQ=255)

                                                                   gcgcGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCt                                                                                    <  2:2300839/150‑1 (MQ=255)

                                                                   gcgcGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCt                                                                                    <  1:1092102/150‑1 (MQ=255)

                                                                    cgcgTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTg                                                                                   >  1:4959622/1‑150 (MQ=255)

                                                                      cgTCTTCGGCGCGCGTTTGCGTACGGTCCTTAGCCGGCGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGt                                                                                 <  1:3961884/150‑1 (MQ=255)

                                                                       gTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTa                                                                                >  1:4708969/1‑150 (MQ=255)

                                                                       gTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTa                                                                                >  1:139879/1‑150 (MQ=255)

                                                                       gTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTa                                                                                <  2:3024475/150‑1 (MQ=255)

                                                                        tCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCTTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAg                                                                               <  1:2918360/150‑1 (MQ=255)

                                                                        tCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCTTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAg                                                                               >  1:1304703/1‑150 (MQ=255)

                                                                         cgtCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGc                                                                              <  2:3955912/148‑1 (MQ=255)

                                                                           tCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGccc                                                                            <  2:1107752/150‑1 (MQ=255)

                                                                             ggCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCTTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTc                                                                          >  1:545599/1‑150 (MQ=255)

                                                                             ggCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTc                                                                          >  1:3011655/1‑150 (MQ=255)

                                                                                  gcgTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCAcc                                                                     <  1:3241358/150‑1 (MQ=255)

                                                                                      ttGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGct                                                                 >  1:1503408/1‑150 (MQ=255)

                                                                                      ttGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGct                                                                 <  2:1697179/150‑1 (MQ=255)

                                                                                       tGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGctc                                                                >  2:4579569/1‑150 (MQ=255)

                                                                                             cggTCCTTCGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCa                                                          <  1:2721971/148‑1 (MQ=255)

                                                                                              tGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCAt                                                         <  1:3298952/150‑1 (MQ=255)

                                                                                               gTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCAtt                                                        >  2:3941159/1‑150 (MQ=255)

                                                                                                tCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATtg                                                       <  2:1434656/150‑1 (MQ=255)

                                                                                                 ccTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATtgt                                                      >  1:1011526/1‑150 (MQ=255)

                                                                                                  cTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATtgtg                                                     >  2:1316903/1‑150 (MQ=255)

                                                                                                    tAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCt                                                   >  1:2337903/1‑150 (MQ=255)

                                                                                                     aGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTg                                                  <  1:3681987/150‑1 (MQ=255)

                                                                                                      gCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCTTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTgg                                                 <  1:490822/150‑1 (MQ=255)

                                                                                                       ccGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTggg                                                >  2:3397629/1‑150 (MQ=255)

                                                                                                         ggAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTg                                              <  1:3317485/150‑1 (MQ=255)

                                                                                                          gAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGc                                             >  1:2528160/1‑150 (MQ=255)

                                                                                                           aGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCa                                            <  2:4996335/150‑1 (MQ=255)

                                                                                                                 gAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTc                                      >  2:4121511/1‑150 (MQ=255)

                                                                                                                 gAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTc                                      <  2:1032847/150‑1 (MQ=255)

                                                                                                                   aaaaaaGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCTCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCgg                                    >  2:1793554/1‑150 (MQ=255)

                                                                                                                   aaaaaaGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCgg                                    >  2:2098908/1‑150 (MQ=255)

                                                                                                                    aaaaaGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCggg                                   <  1:3844336/150‑1 (MQ=255)

                                                                                                                      aaaGCCGCTCGCCTTTTGCGCGGGGATCGGCTTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGAc                                 >  2:2041075/1‑150 (MQ=255)

                                                                                                                        aGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACgg                               >  1:3517257/1‑150 (MQ=255)

                                                                                                                          ccGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGgcg                             <  1:5024468/150‑1 (MQ=255)

                                                                                                                            gCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCt                           <  2:922876/150‑1 (MQ=255)

                                                                                                                            gCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCt                           >  1:1889157/1‑150 (MQ=255)

                                                                                                                             cTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTa                          <  2:2868686/150‑1 (MQ=255)

                                                                                                                              tCGCCTTTTGCGCGGGGATCGGCTTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTaa                         <  2:2949620/150‑1 (MQ=255)

                                                                                                                              tCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTaa                         <  1:2615574/150‑1 (MQ=255)

                                                                                                                              tCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTaa                         <  2:2536448/150‑1 (MQ=255)

                                                                                                                                gCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATg                       <  2:3688460/150‑1 (MQ=255)

                                                                                                                                gCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATg                       <  1:1121509/150‑1 (MQ=255)

                                                                                                                                gCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATg                       >  2:3075137/1‑150 (MQ=255)

                                                                                                                                 ccTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATgg                      >  1:2155743/1‑150 (MQ=255)

                                                                                                                                  cTTTTGCGCGGGGATCGGCTTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATggt                     <  1:444387/150‑2 (MQ=255)

                                                                                                                                  cTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATGgc                     >  1:992961/1‑150 (MQ=255)

                                                                                                                                        cgcgGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATGGCGCGTAt               >  2:2233668/1‑150 (MQ=255)

                                                                                                                                           ggggATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATGGCGCGTATtct            <  1:681971/150‑1 (MQ=255)

                                                                                                                                           ggggATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATGGCGCGTATtct            >  2:835141/1‑150 (MQ=255)

                                                                                                                                             ggATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATGGCGCGTATTCTCa          >  1:3701444/1‑150 (MQ=255)

                                                                                                                                                tCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATGGCGCGTATTCTCACCt       >  2:4921225/1‑150 (MQ=255)

                                                                                                                                                 cGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATGGCGCGTATTCTCACCtt      <  1:1583926/150‑1 (MQ=255)

                                                                                                                                                  ggCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATGGCGCGTATTCTCACCTTc     <  2:4736131/150‑1 (MQ=255)

                                                                                                                                                   gCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATGGCGCGTATTCTCACCTTCg    >  2:3659758/1‑150 (MQ=255)

                                                                                                                                                     gTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATGGCGCGTATTCTCACCTTCGAc  >  2:5060618/1‑150 (MQ=255)

TGCTTCACCTGCTTTCTGCCGTCGCCCTGCTGGTCTGGGGGACTCATATTGTTCGAACCGGCGTAATGCGCGTCTTCGGCGCGCGTTTGCGTACTGTCCTTAGCCGGAGCGTCGAAAAAAAGCCGCTCGCCTTTTGCGCGGGGATCGGCGTTACCGCACTGGTACAGAGCAGTAATGCCACCACCATGCTGGTGACCTCGTTTGTCGCTCAGGATCTGGTAGCCCTCGCACCGGCTCTGGTCATTGTGCTGGGTGCAGATGTCGGGACGGCGCTAATGGCGCGTATTCTCACCTTCGAC  >  CP009273/4217669‑4217967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A