Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F26 I0 R1 94 331.7 11075696 93.7% 10377927 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 295,695 T→G 18.2% E317D (GAA→GAC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273295,6950TG18.2% 865.5 / 74.1 377E317D (GAA→GAC) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base T (125/175);  new base G (75/2);  total (200/177)
Fisher's exact test for biased strand distribution p-value = 1.04e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 382 total aligned reads displayed.

CGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATC  >  CP009273/295547‑295842
                                                                                                                                                    |                                                                                                                                                   
cgtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGttt                                                                                                                                                    <  1:562239/150‑1 (MQ=255)
   cgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCg                                                                                                                                                 >  1:3548375/1‑150 (MQ=255)
     aTCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGc                                                                                                                                               >  2:88067/1‑150 (MQ=255)
      tCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCg                                                                                                                                              <  2:4223583/150‑1 (MQ=255)
       cgcgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCggggg                                                                                                                                             >  1:5194296/1‑147 (MQ=255)
         cgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgc                                                                                                                                           >  2:2766955/1‑150 (MQ=255)
         cgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGgc                                                                                                                                           >  1:3821362/1‑150 (MQ=255)
          gATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTgcc                                                                                                                                          >  1:1820387/1‑150 (MQ=255)
            tttCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGGgccgc                                                                                                                                        >  2:3724270/1‑150 (MQ=255)
                gAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCggg                                                                                                                                    <  2:4289507/150‑1 (MQ=255)
                gAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCggg                                                                                                                                    >  1:3733943/1‑150 (MQ=255)
                   cgcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAg                                                                                                                                 >  2:754308/1‑150 (MQ=255)
                   cgcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTACGGCGGGCCGCCcgggac                                                                                                                                 >  1:2630301/1‑147 (MQ=255)
                      cATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGGCgccgggccgggt                                                                                                                              >  1:4652535/1‑148 (MQ=255)
                       aTCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCCGGCAGGttt                                                                                                                             >  1:1280310/1‑150 (MQ=255)
                        tCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTc                                                                                                                            <  1:952560/150‑1 (MQ=255)
                        tCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTc                                                                                                                            <  1:899026/150‑1 (MQ=255)
                         cAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCCGGCAGGtttac                                                                                                                           >  1:2177671/1‑148 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCa                                                                                                                          >  2:3438827/1‑150 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTACa                                                                                                                          >  1:3999097/1‑150 (MQ=255)
                           gACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAg                                                                                                                         <  1:1329500/150‑1 (MQ=255)
                             ccGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGAc                                                                                                                       <  1:1529270/150‑1 (MQ=255)
                               gggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCa                                                                                                                     <  2:3692255/150‑1 (MQ=255)
                                  ggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCt                                                                                                                  <  2:3188460/150‑1 (MQ=255)
                                    cTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCGCCGGGCAGGTTTCCAGACCAGCgtt                                                                                                                >  2:4722376/1‑150 (MQ=255)
                                     ttCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTc                                                                                                               <  2:2217589/150‑1 (MQ=255)
                                          cGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAtgtg                                                                                                          >  2:1240299/1‑150 (MQ=255)
                                           gggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGGGCCGCCGGGGCGGGTTGCAGACCACCGTTCAAGTga                                                                                                         >  1:3443392/1‑150 (MQ=255)
                                            ggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCAGGGTTGCAGACCAGCTTTAATGTgag                                                                                                        >  1:2715223/1‑150 (MQ=255)
                                             gcgcACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTgaga                                                                                                       >  2:2857858/1‑150 (MQ=255)
                                                cacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGCTAg                                                                                                    >  2:3824592/1‑150 (MQ=255)
                                                cacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAg                                                                                                    <  1:2222420/150‑1 (MQ=255)
                                                cacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGTGCCGCCGGGCCGGTTTCCAGACCCACTGTAATGTGTGATAg                                                                                                    >  1:38578/1‑150 (MQ=255)
                                                    caTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGGGCCGCCGGGCCGGTTTCCAGACCAGCGTTCATGGGAGATAGCggg                                                                                                >  2:660693/1‑149 (MQ=255)
                                                      tGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGa                                                                                              <  1:4068017/150‑1 (MQ=255)
                                                      tGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGGGATAGCGGTGa                                                                                              >  2:263883/1‑150 (MQ=255)
                                                         cGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCCGGGTTCCAGACCAGCGTTCATGTGAGATAGCGGTGtttt                                                                                           >  2:5148653/1‑150 (MQ=255)
                                                          gTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAGGTGAGATAGCGGTGAtttt                                                                                          >  2:3995243/1‑150 (MQ=255)
                                                          gTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCCCCGGGGCGGGTTCCAGACCCGCGTTCAAGTGAGATAGCGGGGGtttt                                                                                          >  1:641806/1‑150 (MQ=255)
                                                           ttCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTc                                                                                         >  2:2081845/1‑150 (MQ=255)
                                                            tCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTcc                                                                                        <  2:2453480/150‑1 (MQ=255)
                                                            tCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGTGCCGCCGGGCCGGTTTCCAGACCAGCTTGAATGTGAGATAGCGGTGAttttta                                                                                        >  1:4840659/1‑148 (MQ=255)
                                                                ttCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCCGGTTTCCAGACCAGCTTTAATGTGAGATAGCGGGGATTTTACCGct                                                                                    >  2:2029527/1‑150 (MQ=255)
                                                                  ccGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGctct                                                                                  <  2:5201379/150‑1 (MQ=255)
                                                                  ccGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGctct                                                                                  <  2:4646490/150‑1 (MQ=255)
                                                                  ccGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGctct                                                                                  <  1:3176845/150‑1 (MQ=255)
                                                                     gCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGt                                                                               <  2:5373450/150‑1 (MQ=255)
                                                                     gCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCGGGCCGGTTTCCAGACCCGCGTTCAGGTGTGCTAGCGGTGGTTTTACCGcgcgggg                                                                               >  2:15013/1‑144 (MQ=255)
                                                                       aaaTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGAACAGCTTTCATGTGAGATAGCGGTGATTTTACCGCGCTTGTCg                                                                             >  2:4559452/1‑150 (MQ=255)
                                                                          tCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCa                                                                          <  2:2918332/150‑1 (MQ=255)
                                                                              gCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGa                                                                      <  2:845709/150‑1 (MQ=255)
                                                                               cGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGGGCCGCCGGGGCGGTTTGCACACCCGCTTTCATGTGAGGTAGCGGTGCTTTTCCCGCGCTGGTCGGCACCGAt                                                                     >  2:5330939/1‑150 (MQ=255)
                                                                               cGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGAt                                                                     <  1:4485326/150‑1 (MQ=255)
                                                                               cGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGAt                                                                     <  1:3631473/150‑1 (MQ=255)
                                                                                ggggCTAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATa                                                                    <  2:1284904/148‑1 (MQ=255)
                                                                                gTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCACGCTCTGGTCGGCACCGATa                                                                    >  2:238162/1‑150 (MQ=255)
                                                                                 tGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATAc                                                                   <  1:2912487/150‑1 (MQ=255)
                                                                                 tGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTACCGCTCTGGTCGGCACCGATaa                                                                   >  2:2741360/1‑149 (MQ=255)
                                                                                          gCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCt                                                          <  1:438092/150‑1 (MQ=255)
                                                                                           cAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTg                                                         <  1:5220408/150‑1 (MQ=255)
                                                                                            aGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGa                                                        >  2:4135856/1‑150 (MQ=255)
                                                                                            aGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCCGGCACGTTTACAGACCACCGTTGAAGTGAGATAGCGGTGATTTTAACGCACAGGTCGGCACCCAAAAGCAAGTGCTGa                                                        >  1:2258530/1‑150 (MQ=255)
                                                                                            aGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCGCCGGGCAGGTTTACAGACCAGCCTTCATGTGAGATAGCGGTGATTTTACCGCGCGGGTCGGCACCGAAACCCAAGTGCTGa                                                        >  2:1594966/1‑150 (MQ=255)
                                                                                                 ggTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTACGGCGTGCCGCCCGGCAGGGTTACAGACCCGCGTTCAAGTGTGATAGCGGGGATTTTACCGCTCTGGTAGGCACCGATACGCCACTGCTGTAGGGt                                                   >  2:1075470/1‑150 (MQ=255)
                                                                                                 ggTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTGGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGt                                                   <  2:478691/150‑1 (MQ=255)
                                                                                                  gTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTg                                                  <  1:2476880/150‑1 (MQ=255)
                                                                                                          aTTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCCGCGGGCCGCCGGGCAGGTTTACAGACCAGCTTTAATTTTAGATATCGGTGATTTTCCCCCTCTGGTCGGCACCCAAAAGCAAGTGCAGAACGGGggcgggggg                                          >  1:4573644/1‑148 (MQ=255)
                                                                                                             cGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTg                                       <  2:3415195/150‑1 (MQ=255)
                                                                                                             cGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTg                                       <  1:2375182/150‑1 (MQ=255)
                                                                                                             cGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCAGGTTTACAGACCAGCTTTCATGTGAGATAGCGGTGATTTTACCCCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTggcggggcgggg                                       >  2:2278423/1‑148 (MQ=255)
                                                                                                             cGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTACGGCGTGCCGCCGGGCAGGTTTACAGACCAGCGTTCATGTGAGATAGCGGTGATTTTACCGCTCTTGTCGGCACCCATAAGCAAGTTCTGAAGGGTGGCGGGGGGGTg                                       >  2:61871/1‑150 (MQ=255)
                                                                                                              gCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCCGGCCGGTTTACAGACCCGCTTTAATGTGAGATAGCGGTGATTTTACCCCTCTGGTCGGCACCGCTACGCAAGTGCTGAATGGTGGCggggcggggc                                      >  2:2506470/1‑150 (MQ=255)
                                                                                                                  cGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGGGCCCCCCGGCACGGTTACACACCACCTTTCAAGTGAGATAGCGGTGATTTTACCGCGCGGGTCGGCCCCGCTACGCCAGAGCTGAACGGTGGcggggcggggcgggg                                  >  2:4807104/1‑150 (MQ=255)
                                                                                                                   ggCGTAGAGTAATTCGCTTAGCTGTACCGCCGTTTACGGCGTTCCGCCCGGCACGTTTCCACAACATCTTTAATGAGAGATATCGGTGATTTTACCGCTCTGGTCGGCAACGATACCCAACTGCAGAAGGGTGGCGGGGCGGTGCgtggt                                 >  2:906201/1‑150 (MQ=255)
                                                                                                                    gCGTAGAGTAATTCGCTGTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggtg                                <  2:4463761/150‑1 (MQ=255)
                                                                                                                       tAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTa                             <  1:494323/150‑1 (MQ=255)
                                                                                                                       tAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTa                             <  1:1372297/150‑1 (MQ=255)
                                                                                                                       tAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCCGCGGGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTACCCCTCAGGTCGGCACCGATAAGCAAGTGCTGAAGGGTTGCGGGGCGGGGGGGGGTGTTa                             >  2:722330/1‑150 (MQ=255)
                                                                                                                       tAGAGTAAGTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTa                             <  2:3122073/150‑1 (MQ=255)
                                                                                                                        agagTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAt                            <  2:2887590/150‑1 (MQ=255)
                                                                                                                        agagTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAt                            <  2:2180957/150‑1 (MQ=255)
                                                                                                                        agagTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTAATGTGTGATAGCGGTGATTTTACCGCTCTGGTCGGCACCGATACGCAATTGGTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAt                            >  2:3642014/1‑150 (MQ=255)
                                                                                                                        agagTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCCCCGGGCACGTTTACAGACCACCTTTCATGTGCGCTAGCGGTGGTTTTACCGCTCAGGTCGGCACCCATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGGGGTGTTAt                            >  2:190044/1‑150 (MQ=255)
                                                                                                                        agagTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCGTTCATGTGAGATAGCGGTGATTTTACCGCGCTGGTCGGCACCGATAAGCCAGTGCGGAAGGGTGGCGGGGCGGGGCGTGGTGTTAt                            >  2:3958007/1‑150 (MQ=255)
                                                                                                                        agagTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTTCCCCCCGGCAGGTTTCCAGCACACCTTTAAATTGAGATAGCGGTGATTTTACCCCTCTGGTCGGCACCCATACCCAACTGGTGAACGGTGGCGGGGCGGTGCGTGGTGTTaa                            >  2:3299158/1‑149 (MQ=255)
                                                                                                                         gagTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAtt                           <  2:4961663/150‑1 (MQ=255)
                                                                                                                           gTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTgg                         <  2:3848949/150‑1 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCTGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCTCTCTGGTCGGCACCGATACTCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAAtt                    <  2:953336/150‑2 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATa                    <  1:2702817/150‑1 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATa                    <  1:1871695/150‑1 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATa                    <  1:1055894/150‑1 (MQ=255)
                                                                                                                                     ttGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAggg               <  1:1903555/150‑1 (MQ=255)
                                                                                                                                        cTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCg            <  1:1240299/150‑1 (MQ=255)
                                                                                                                                             ccgccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGa       >  2:1943511/1‑150 (MQ=255)
                                                                                                                                               gccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGc     <  1:2550583/150‑1 (MQ=255)
                                                                                                                                                ccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCa    <  1:5333277/150‑1 (MQ=255)
                                                                                                                                                  gTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATc  >  2:4839825/1‑150 (MQ=255)
                                                                                                                                                  gTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATc  >  2:3880956/1‑150 (MQ=255)
                                                                                                                                                  gTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATc  >  2:3545193/1‑150 (MQ=255)
                                                                                                                                                  gTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATc  >  1:4178457/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATC  >  CP009273/295547‑295842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A