Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 295,695 T→G 18.1% E317D (GAA→GAC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273295,6950TG18.1% 367.9 / 47.6 211E317D (GAA→GAC) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base T (49/120);  new base G (39/3);  total (88/123)
Fisher's exact test for biased strand distribution p-value = 1.25e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

CGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCA  >  CP009273/295547‑295843
                                                                                                                                                     |                                                                                                                                                      
cgtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGt‑t                                                                                                                                                        <  2:1933325/149‑1 (MQ=255)
 gtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGt‑tt                                                                                                                                                       <  1:1646801/149‑1 (MQ=255)
 gtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGt‑tt                                                                                                                                                       >  2:621277/1‑149 (MQ=255)
   cgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTcc                                                                                                                                                     >  2:2795494/1‑149 (MQ=255)
     aTCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCgg                                                                                                                                                   >  1:3401653/1‑149 (MQ=255)
      tCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGc                                                                                                                                                  >  2:446231/1‑149 (MQ=255)
        gcgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGt                                                                                                                                                <  1:2485064/149‑1 (MQ=255)
        gcgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGt                                                                                                                                                <  1:2444234/149‑1 (MQ=255)
         cgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑g                                                                                                                                              <  1:1074924/149‑1 (MQ=255)
           aTTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑gcc                                                                                                                                            >  2:2299830/1‑149 (MQ=255)
           aTTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTCCGGCGT‑gcc                                                                                                                                            >  2:2185112/1‑149 (MQ=255)
                gAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCgg                                                                                                                                      >  2:4081456/1‑149 (MQ=255)
                gAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTCCGGCGT‑GCCG‑CCgg                                                                                                                                      >  2:2484277/1‑149 (MQ=255)
                    gcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAg                                                                                                                                  <  2:2727041/149‑1 (MQ=255)
                    gcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAg                                                                                                                                  <  2:623025/149‑1 (MQ=255)
                     ccATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAgg                                                                                                                                 >  1:315622/1‑149 (MQ=255)
                      cATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGt                                                                                                                                >  2:2613752/1‑149 (MQ=255)
                       aTCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTGCGGCGG‑GCCG‑CCCGGCCGGtt                                                                                                                               >  1:3145079/1‑149 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTcc                                                                                                                            <  1:3232353/149‑1 (MQ=255)
                           gACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCa                                                                                                                           <  2:3337862/149‑1 (MQ=255)
                               gggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGAcc                                                                                                                       <  2:1808796/149‑1 (MQ=255)
                                ggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTTCGGCGG‑GGCG‑CCGGGCCGGGGTGCAGACCa                                                                                                                      >  1:828986/1‑149 (MQ=255)
                                ggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGAccc                                                                                                                      >  2:2286125/1‑148 (MQ=255)
                                    cTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCtt                                                                                                                  <  2:3660866/149‑1 (MQ=255)
                                     ttCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCttt                                                                                                                 <  1:122757/149‑1 (MQ=255)
                                     ttCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTGGGGCGG‑GGCG‑CCGGGCCGGGTTTCAGACCaccgtt                                                                                                                 >  1:1152543/1‑144 (MQ=255)
                                     ttCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTGCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCttt                                                                                                                 >  2:2563350/1‑149 (MQ=255)
                                      tCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTc                                                                                                                <  1:1729293/149‑1 (MQ=255)
                                        gCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCAt                                                                                                              <  2:53679/149‑1 (MQ=255)
                                           gggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTAGGACGATTCGCCCCGGCGTAGAGTACTTCGCTTTGCTGTACCGCCGT‑GTCCGGCGG‑GCCG‑CCGGGCAGGGTTCCAGACCCGCGTGCCtgtg                                                                                                           >  2:248702/1‑149 (MQ=255)
                                            ggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTCCATGTga                                                                                                          <  2:3549997/149‑1 (MQ=255)
                                              cgcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTgaga                                                                                                        <  2:3397122/149‑1 (MQ=255)
                                               gcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGAt                                                                                                       <  2:2934072/149‑1 (MQ=255)
                                                cacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATa                                                                                                      <  1:903680/149‑1 (MQ=255)
                                                     aTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGGGGG‑GCGG‑CCGGGC‑GGGTTGCACACCACCGTTCAAGTGGGATTGCggg                                                                                                 >  1:3462155/1‑148 (MQ=255)
                                                     aTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTGCGGCGG‑GCCG‑CCGGGCCGGGTTGCAGACCCCCCTTAAAGTGAGATTGCggg                                                                                                 >  1:2185577/1‑148 (MQ=255)
                                                      tGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTg                                                                                                <  1:176312/149‑1 (MQ=255)
                                                      tGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCATTCATGTGAGATAGCGGTg                                                                                                >  2:555635/1‑149 (MQ=255)
                                                          gTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTGCGGCGG‑GCCG‑CCGGGGCGGTTTCCCCACCACCCTTCATGTGGGATATCGGGGtttt                                                                                            >  1:4019652/1‑149 (MQ=255)
                                                           ttCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGAtttt                                                                                           <  2:526812/149‑1 (MQ=255)
                                                           ttCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGAtttt                                                                                           <  2:195176/149‑1 (MQ=255)
                                                           ttCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCCGCGT‑GCCG‑CCGCGCAGGGTTACAGACCAGCTTTCATGTGATATATACGTGAtttt                                                                                           >  2:3026884/1‑149 (MQ=255)
                                                           ttCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTGCGGGCG‑GGCG‑CCGGGGCGGGTTGCACACCAGCGTTAATGTGTGGAGGCGGTGAtttt                                                                                           >  1:1487118/1‑149 (MQ=255)
                                                            tCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTc                                                                                          <  1:1129929/149‑1 (MQ=255)
                                                            tCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTCCGGCGG‑GCCG‑CCGGGCAGGGTTCCAGACCAGCGTTCATGTGAGATAGCGGGGGtttta                                                                                          >  2:3793527/1‑148 (MQ=255)
                                                              ccTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTccc                                                                                        <  2:315622/149‑1 (MQ=255)
                                                              ccTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTccc                                                                                        <  1:102309/149‑1 (MQ=255)
                                                              ccTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTccc                                                                                        <  1:2177975/149‑1 (MQ=255)
                                                              ccTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTGCGGCGG‑GCCG‑CCGGGCAGGTGTCCAGACCCCCGTTCATGTGAGGTATCGGGGCTTTTtcc                                                                                        >  2:361913/1‑149 (MQ=255)
                                                               cTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTGCGGCGT‑GCCG‑CCCGGCCGGTTTCCAGACCACCGTTGATGTGAGATAGCGGGGGTTTTcccc                                                                                       >  1:2522594/1‑148 (MQ=255)
                                                                  ccGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGctc                                                                                    <  1:3544097/149‑1 (MQ=255)
                                                                    ggCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTg                                                                                  <  1:2377887/149‑1 (MQ=255)
                                                                     gCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTCCCGCGT‑GGCGCCCGGGCCGG‑TTACAGCCCACCATTCATGTGGGTTAGCCGGGGTTTTACCGCGCTgg                                                                                 >  1:3005166/1‑149 (MQ=255)
                                                                       aaaTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTCCGGCGT‑GCCC‑CCGGGCACGTTTCCAGACCAGCTTTAAAGTGAGATAGCGGTGATTTTGCCGCGCTGGTc                                                                               >  2:475453/1‑149 (MQ=255)
                                                                             aGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCACCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCAcc                                                                         >  2:2340787/1‑149 (MQ=255)
                                                                                        ccGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGt                                                              <  1:281075/149‑1 (MQ=255)
                                                                                             ggCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGAtttt                                                                                           <  1:2705432/115‑1 (MQ=255)
                                                                                             ggCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGAtttt                                                                                           >  2:2705432/1‑115 (MQ=255)
                                                                                             ggCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCGTTCATGTGAGATAGCGGTGATTTTACCGCTCTGGTCGGCACCGATACGCAAGTGCTGa                                                         >  2:1352505/1‑149 (MQ=255)
                                                                                                 ggTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTGCGGCGTGGCCC‑CCGGGCCGG‑TTGCAGAACCCCCTTGCTGTGAGTTATCCGGGATTTTACCGCACGGGTCGGCCCCGAGAAGCCACTGCTGGAggg                                                     >  1:2228696/1‑149 (MQ=255)
                                                                                                 ggTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAggg                                                     <  1:1343069/149‑1 (MQ=255)
                                                                                                  gTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGt                                                    <  2:3352864/149‑1 (MQ=255)
                                                                                                  gTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTGTGCTGTACCGCCGT‑GTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGt                                                    <  2:3443774/149‑1 (MQ=255)
                                                                                                   tATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTCCGGCGT‑GCCG‑CCGGGCCGGTTTCCAGACCAGCGTTCATGTGAGATAGCGGTGATTTTCCCGCGCGGGTCGGCCCCGATACGCAAGTGCTGAAggggg                                                   >  2:2952594/1‑147 (MQ=255)
                                                                                                    aTGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑GTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTgg                                                  <  2:3421909/149‑1 (MQ=255)
                                                                                                     tGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTggc                                                 <  1:2382384/149‑1 (MQ=255)
                                                                                                     tGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTggc                                                 <  1:2299830/149‑1 (MQ=255)
                                                                                                      gACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTggcg                                                <  2:1898308/149‑1 (MQ=255)
                                                                                                          aTTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTggcggggc                                            <  1:456550/149‑1 (MQ=255)
                                                                                                             cGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGt                                         <  1:3809237/149‑1 (MQ=255)
                                                                                                             cGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGt                                         <  2:1077324/149‑1 (MQ=255)
                                                                                                              gCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTg                                        <  2:3436522/149‑1 (MQ=255)
                                                                                                               ccccGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTGCGGCGT‑GCCG‑CCGGGCCGGTTTGCCGACCACCCTTCATTTGTGATATCGGGGATTTTACCGCGCTGGTCGGGACCGAGACGCAAGTGCTGAAGGGTGGGGGGGCGGTgg                                       >  2:3874929/1‑148 (MQ=255)
                                                                                                                 ccGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgt                                     <  1:2386878/149‑1 (MQ=255)
                                                                                                                  cGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtg                                    <  1:2063723/149‑1 (MQ=255)
                                                                                                                  cGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtg                                    <  1:486853/149‑1 (MQ=255)
                                                                                                                  cGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtg                                    <  1:486915/149‑1 (MQ=255)
                                                                                                                   ggCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtgg                                   <  1:1994477/149‑1 (MQ=255)
                                                                                                                     cGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggtg                                 <  1:4109687/149‑1 (MQ=255)
                                                                                                                         gagTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑TCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAt                             <  1:3173359/149‑1 (MQ=255)
                                                                                                                           gTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTg                           <  2:888285/149‑1 (MQ=255)
                                                                                                                             aaTTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTACCGCTCTGGTCGGCACCGATAAGCAAGTGCTGAAGGGTGGCGGGGGGGGGGGGGGGGTTATTggg                         >  2:1006620/1‑149 (MQ=255)
                                                                                                                              aTTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGa                        <  2:2502180/149‑1 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑TCCG‑CCGGGCAGGTTTCCAGAACAGCGTTCATTTGGGATAGCGGTGATTTTAACGCGCTGGTCGGCCCCGCTAAGCAAGTGCTGAAGGGTGGCGGGGGGGTGGGGGGGGTTATTGGGact                      >  2:545148/1‑147 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAAt                      <  2:828986/149‑1 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAAt                      <  1:1844276/149‑1 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGG‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAAt                      <  1:985262/149‑1 (MQ=255)
                                                                                                                                 cGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCCGGTTTGCAGAAAAGCTTTCATGTGAGATAGCGGTGATTTTACCCCGCTGGTCGGCACCGATACCCCAGTGCTGAAGGGTGGCGGGGCGGTGGGGGGGGGTATTGGGaaaa                     >  2:1509322/1‑147 (MQ=255)
                                                                                                                                 cGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATa                     <  1:3147852/149‑1 (MQ=255)
                                                                                                                                   cTTTGCTGTACCTCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATaga                   <  1:3957588/149‑1 (MQ=255)
                                                                                                                                   cTTTGCTGTACCGCCGT‑GTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGTTATAGCGGTGATTTTGCCCCTCTGGGGGGCACCGATAAGCCCGTGCTGAAGGGTGGGGGGGCGGGGGGGGGGGTTCTTggggagagt                   >  2:4006067/1‑143 (MQ=255)
                                                                                                                                        cTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGc              <  1:3712732/149‑1 (MQ=255)
                                                                                                                                             ccgccgT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCggggg         <  1:4081456/149‑1 (MQ=255)
                                                                                                                                             ccgccgT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCggggg         <  1:2484277/149‑1 (MQ=255)
                                                                                                                                              cgccgT‑TTCCGGCGT‑GCCG‑CCGGGCTGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGa        <  1:1006620/149‑1 (MQ=255)
                                                                                                                                                ccgT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGGGc      >  1:3944123/1‑149 (MQ=255)
                                                                                                                                                 cgT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCa     <  2:3101735/149‑1 (MQ=255)
                                                                                                                                                   t‑ttCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATc   >  1:2831752/1‑149 (MQ=255)
                                                                                                                                                   t‑ttCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATc   >  2:2619199/1‑149 (MQ=255)
                                                                                                                                                     ttCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCa  <  1:1098450/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGT‑TTCCGGCGT‑GCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCA  >  CP009273/295547‑295843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A