Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F17 I0 R1 93 279.2 9183790 95.6% 8779703 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 295,695 T→G 17.0% E317D (GAA→GAC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273295,6950TG17.0% 619.2 / 53.3 289E317D (GAA→GAC) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base T (71/162);  new base G (54/2);  total (125/164)
Fisher's exact test for biased strand distribution p-value = 4.31e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 301 total aligned reads displayed.

CGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATC  >  CP009273/295547‑295842
                                                                                                                                                    |                                                                                                                                                     
cgtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGtt                                                                                                                                                       <  2:2933971/149‑1 (MQ=255)
cgtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGtt                                                                                                                                                       <  1:3647734/149‑1 (MQ=255)
 gtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGttt                                                                                                                                                      >  1:4181575/1‑149 (MQ=255)
 gtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATAAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGttt                                                                                                                                                      >  1:3857873/1‑149 (MQ=255)
     aTCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCgg                                                                                                                                                  >  1:3965049/1‑149 (MQ=255)
        gcgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGt                                                                                                                                               >  1:2651852/1‑149 (MQ=255)
          gATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgc                                                                                                                                             <  1:1034277/149‑1 (MQ=255)
           aTTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgcc                                                                                                                                            <  1:3861071/149‑1 (MQ=255)
           aTTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTgcc                                                                                                                                            >  2:2116140/1‑149 (MQ=255)
             ttCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTgccgc                                                                                                                                          <  2:101284/149‑1 (MQ=255)
                gAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCgg                                                                                                                                       <  1:4381893/149‑1 (MQ=255)
                     ccATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAgg                                                                                                                                  <  1:436060/149‑1 (MQ=255)
                       aTCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGtt                                                                                                                                <  2:2570071/149‑1 (MQ=255)
                        tCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGttt                                                                                                                               >  2:2846339/1‑149 (MQ=255)
                        tCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGGCGCCGGGCAGGttt                                                                                                                               >  1:1877060/1‑149 (MQ=255)
                         cAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTc                                                                                                                              <  2:4219736/149‑1 (MQ=255)
                         cAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTc                                                                                                                              <  1:3416865/149‑1 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGCCGCCCGGCAGGTTTcc                                                                                                                             >  1:2587131/1‑149 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTcc                                                                                                                             <  2:2442606/149‑1 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCCGCGGGGCCCCGGGCCggggtgc                                                                                                                             >  1:1625010/1‑144 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCCCCCGGCCGGgttac                                                                                                                             >  1:2237250/1‑147 (MQ=255)
                             ccGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGa                                                                                                                          <  2:269051/149‑1 (MQ=255)
                             ccGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGa                                                                                                                          <  2:626259/149‑1 (MQ=255)
                             ccGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGa                                                                                                                          <  2:3741656/149‑1 (MQ=255)
                             ccGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGa                                                                                                                          <  2:2366368/149‑1 (MQ=255)
                               gggtgCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGAcc                                                                                                                        <  1:182557/149‑1 (MQ=255)
                                   gCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCCGGGTTCCAGACCACCt                                                                                                                    >  2:3385896/1‑149 (MQ=255)
                                    cTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCtt                                                                                                                   <  2:2794749/149‑1 (MQ=255)
                                         ccGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAtg                                                                                                              <  1:2513921/149‑1 (MQ=255)
                                          cGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAtgt                                                                                                             <  1:179828/149‑1 (MQ=255)
                                           gggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCCGGGTTGCAGACCAGCGTTCAtgtg                                                                                                            >  2:151117/1‑149 (MQ=255)
                                           gggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAtgtg                                                                                                            <  1:198270/149‑1 (MQ=255)
                                           gggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAtgtg                                                                                                            <  1:2489825/149‑1 (MQ=255)
                                           gggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCGCCGGGCCGGGTTCCAGACCAGCTTTCAtgtg                                                                                                            >  2:3592953/1‑149 (MQ=255)
                                            ggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTACAGACCAGCTTTCATGTga                                                                                                           >  2:476921/1‑149 (MQ=255)
                                             gcgcACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGGTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTga‑g                                                                                                         <  2:2658414/149‑1 (MQ=255)
                                              cgcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCCGCTTTCATGTga‑ga                                                                                                        >  2:2201537/1‑149 (MQ=255)
                                              cgcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTga‑ga                                                                                                        <  1:1305907/149‑1 (MQ=255)
                                              cgcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCGGGCCGGTTTGCAGACCAGCATTAAtgtgtgg                                                                                                         >  1:1022117/1‑146 (MQ=255)
                                               gcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GAt                                                                                                       <  1:1492140/149‑1 (MQ=255)
                                                cacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGCCGCCCGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATa                                                                                                      >  2:1424779/1‑149 (MQ=255)
                                                       ggCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGa                                                                                               <  2:1847840/149‑1 (MQ=255)
                                                        gCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAAGTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGAt                                                                                              <  1:3559426/149‑1 (MQ=255)
                                                          gTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCCGCGTGCCGCCGGGCAGGTTTACAGACCAGCTTTCATGTGA‑GATATCGGTGAttt                                                                                            >  2:1038999/1‑149 (MQ=255)
                                                          gTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGGTTACAGACCAGCTTTCATGTGA‑GATAGCGGTGAttt                                                                                            >  2:2570329/1‑149 (MQ=255)
                                                              ccTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGGCGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGGGGCGCCCGGCCGGTTTTGAGACCCCCTTTGAAGTGT‑GATATTGGTGTttttaac                                                                                        >  1:1221957/1‑146 (MQ=255)
                                                                    ggCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCCCCGGGCACGTTTACAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTg                                                                                  >  2:4323450/1‑149 (MQ=255)
                                                                        aaTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGGTTCCCGACCAGCTTTCATGTGA‑GATAGCGGGGATTTTCCCGCTCTGGTC‑g                                                                             >  2:4150999/1‑149 (MQ=255)
                                                                        aaTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCCGCGGGCCGCCGGGCAGGTTTACAGACCCCCATTCAAGTGA‑GATAGCGGTGATTTTCACGCTCTGGTC‑g                                                                             >  2:3695671/1‑149 (MQ=255)
                                                                         aTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCCGGCAGGTTTCCAGACCAGCATTCATGTGA‑GATAGCGGTGTTTTTCCCCCACTGGTC‑gg                                                                            >  1:768443/1‑149 (MQ=255)
                                                                            aaGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCAc                                                                         <  2:4489571/149‑1 (MQ=255)
                                                                            aaGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCAGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGACGCCCGGCAGGTTTACACAAAATCATTCATTTGA‑AATATCGGTTATTTTACAGCAATGGTA‑GGCAc                                                                         >  2:3240413/1‑149 (MQ=255)
                                                                              gCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGGGCCCCCGGGGCGGGTTGCAGACCCCCGTTAATGTGT‑GTTAGCGGGGGTTTTTCCGGGCAGGTC‑GGGAccc                                                                       >  2:774802/1‑148 (MQ=255)
                                                                                gTGGCGAGCCGCCGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGAt                                                                     <  2:915638/149‑1 (MQ=255)
                                                                                      aGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCaa                                                               <  1:1747941/149‑1 (MQ=255)
                                                                                       gCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTACCGCGCTGGTC‑GGCACCGATACGCAAg                                                              >  2:4183754/1‑149 (MQ=255)
                                                                                        ccGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGt                                                             <  1:2329935/149‑1 (MQ=255)
                                                                                           cAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCt                                                          <  2:3507513/149‑1 (MQ=255)
                                                                                           cAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCt                                                          <  1:1700559/149‑1 (MQ=255)
                                                                                             ggCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCACGGTTCCACACCACCTTTAATTTGA‑GATAGCGGTGATTTTACCGCACTGGTG‑GGCACCCATAACCCAGGGCTGa                                                        >  1:642024/1‑149 (MQ=255)
                                                                                             ggCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCCGGCCGGTTTACACCCCACCCGTCATGGGAGGATA‑CGGTGATTTTAACGCACTGGTC‑GGCACCGAAACGCAAATGCTGa                                                        >  2:1153375/1‑149 (MQ=255)
                                                                                             ggCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCACGCGTGCCGCCGGGCCAGTTTAAAGAACAACTTTAATTTGT‑GATATAGGTTATTTTACCCCGCAGGTG‑GGCCCCGATACGCAAGTGGTGa                                                        >  2:1910291/1‑149 (MQ=255)
                                                                                                 ggTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAggg                                                    <  2:3857976/149‑1 (MQ=255)
                                                                                                 ggTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCCGGCCGGTTTACACACCCGCGTTGATGTGT‑GATATCGGGGTTTTTACCGCGCGGGTC‑GGCCCCCATACCCACGTGCTGAAggg                                                    >  2:3890233/1‑149 (MQ=255)
                                                                                                   tATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTg                                                  <  2:3965363/149‑1 (MQ=255)
                                                                                                    aTGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCCCCGGGCAGGTTTCCAGACCAGCATTCAAGTGA‑GATAGCGGTGATTTTCCCGCGCTGGGC‑GGCACCGATAAGCAACTGCTGAAgggggg                                                 >  2:1397276/1‑149 (MQ=255)
                                                                                                     tGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTggc                                                <  1:2784462/149‑1 (MQ=255)
                                                                                                     tGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCCCTAAGCAAGTGCTGAAGGGTggc                                                >  2:3001563/1‑149 (MQ=255)
                                                                                                     tGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCCGGTTTCCAGACCACCTTTCAATTGA‑GAAATCGGTGATTTTGCCGCTCTGGGC‑GGCACCGCTAAGCCAGTGCTGAAGGGTggc                                                >  2:2328755/1‑149 (MQ=255)
                                                                                                      gACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGCGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTggcg                                               >  2:2646102/1‑149 (MQ=255)
                                                                                                          aTTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTggcggggc                                           <  2:54792/149‑1 (MQ=255)
                                                                                                          aTTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGGACCGCCGTGTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTGTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTggcggggc                                           <  2:54917/149‑1 (MQ=255)
                                                                                                           ttCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCGGGCCGGTTTCCAGACCCGCGTTCATGTGA‑GATATCGGTGTTTTTACCGCGCAGGTC‑GGCACCCATAAGCAAGTTCTGAAGGGTggcggggcg                                          >  1:361466/1‑149 (MQ=255)
                                                                                                            tCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTggcggggcgg                                         <  2:3152989/149‑1 (MQ=255)
                                                                                                            tCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTggcggggcgg                                         <  2:1115318/149‑1 (MQ=255)
                                                                                                              gCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCCGCGGGCCGCCGGGCCGGTTTGCAGACCAGCTTTGATGTGA‑GATAGCGGTGATTTTGCCGCACTGGTC‑GGCACCCAAACGCAACTGCGGAAGGGTggcggggcgggg                                       >  2:167404/1‑147 (MQ=255)
                                                                                                                 ccGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTGCAGAACAGCATTCATGGGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAACTGCTGAAGGGTGGCGGGGCGGGGCgt                                    >  2:2632601/1‑149 (MQ=255)
                                                                                                                         gagTAATTCGCTGTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAt                            <  1:551046/149‑1 (MQ=255)
                                                                                                                          agTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAtt                           <  2:161030/149‑1 (MQ=255)
                                                                                                                          agTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAtt                           <  2:2249547/149‑1 (MQ=255)
                                                                                                                          agTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAtt                           <  1:4154212/149‑1 (MQ=255)
                                                                                                                          agTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTAtt                           <  1:4324222/149‑1 (MQ=255)
                                                                                                                           gTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTg                          <  1:3097835/149‑1 (MQ=255)
                                                                                                                           gTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTg                          <  1:2723447/149‑1 (MQ=255)
                                                                                                                              aTTCGCTTTGCTGTACCGCCGTTTCCGACGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATAAGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGa                       >  1:4472867/1‑149 (MQ=255)
                                                                                                                               ttCGCTTTGCTGTACCGCCGTTTGCCGGGGGCCGCCCGGCAGGGTTCCAGACCCGCGGTGAAGGGT‑GTTAGCGGGGATTTTACCGCTCTGGTCGGGCACC‑ATACGCAAGGGGTGAAGGGGGGGGGGGCGGGGGGGGGGGTTTTTGGGag                      >  2:86637/1‑148 (MQ=255)
                                                                                                                               ttCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGaa                      <  2:1276121/149‑1 (MQ=255)
                                                                                                                                tCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAAt                     <  1:2846339/149‑1 (MQ=255)
                                                                                                                                   cTTTGCTGTACCGCCGTTTTCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGT‑GATATCGGTGATTTTACCGCTCTGGTC‑GGGACCCCTACGCAAGTGCGGAAGGGTGGCGGGGCGGTGCGTGGTGTTTTTTGGGATaga                  >  2:2137366/1‑149 (MQ=255)
                                                                                                                                    tttGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATagag                 <  2:3898033/149‑1 (MQ=255)
                                                                                                                                    tttGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATagag                 <  1:484862/149‑1 (MQ=255)
                                                                                                                                        cTGTACCGCCGTGTGCGGCGTGCCCCCCGGCACGTTTACACACCCCCATTAATGTGA‑GATAGCGGTGATTTTACCCCGCGGGTC‑GGCACCCAAAACCCACTGCAGAAGGGTGGCGGGGCGGGGCGGGGGGTTATTGGGGAAAGAGGGGc             >  2:3171934/1‑149 (MQ=255)
                                                                                                                                         tGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCg            <  1:2922167/149‑1 (MQ=255)
                                                                                                                                         tGTACCGCCGTGTCCGGCGGGCCCCCGGGCAGGTTTACAGACCAGCTTTCAAGTGA‑GATATCGGGGGTTTTCCCCCGCAGGTC‑GGCACCGATACCCCAGTGCTGAAGGGTGGCGGGGCGGGGGGTGGGGGTATTGGGCATAGAGGGGCg            >  2:198741/1‑149 (MQ=255)
                                                                                                                                           tACCGCCGTTTACCGCCTGCCCCCCGGCAGGTTTACACAACACCATTAAAGTGA‑GATATCGGTGATTTTACCCCTCAGGTA‑CGCACCCATAACCAACTTCAGAATGGTGGCGGGGCGGTGCGTGGTGTTATTTGGAATAGATGGGCggg          >  2:3006655/1‑149 (MQ=255)
                                                                                                                                              cgccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGa       <  2:4049303/149‑1 (MQ=255)
                                                                                                                                               gccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAg      <  1:3292025/149‑1 (MQ=255)
                                                                                                                                                  gTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCAt   <  2:433131/149‑1 (MQ=255)
                                                                                                                                                  gTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCAt   >  2:825875/1‑149 (MQ=255)
                                                                                                                                                  gTTTCCGGCGTGCCGCCGGGCAGGTTGCCAGACCAGCTTTCAGGTGA‑GATAGCGGTGATTTTCCCGCTCGGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCAt   <  2:1939427/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGA‑GATAGCGGTGATTTTCCCGCTCTGGTC‑GGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATC  >  CP009273/295547‑295842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A