Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 295,695 T→G 14.0% E317D (GAA→GAC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273295,6950TG14.0% 353.0 / 17.3 190E317D (GAA→GAC) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base T (49/110);  new base G (29/2);  total (78/112)
Fisher's exact test for biased strand distribution p-value = 3.37e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

TCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATC  >  CP009273/295549‑295842
                                                                                                                                                  |                                                                                                                                                    
tcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGttt                                                                                                                                                     >  2:82497/1‑148 (MQ=255)
tcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGttt                                                                                                                                                     >  1:313779/1‑148 (MQ=255)
    tCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCgg                                                                                                                                                 >  2:651271/1‑148 (MQ=255)
    tCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCgg                                                                                                                                                 >  2:3116943/1‑148 (MQ=255)
    tCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCgg                                                                                                                                                 >  2:2951660/1‑148 (MQ=255)
    tCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCgg                                                                                                                                                 >  2:2671797/1‑148 (MQ=255)
    tCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCgg                                                                                                                                                 >  2:1043188/1‑148 (MQ=255)
     cgcgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGc                                                                                                                                                <  2:2051127/148‑1 (MQ=255)
     cgcgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGc                                                                                                                                                <  1:3709567/148‑1 (MQ=255)
     cgcgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGc                                                                                                                                                >  1:3605807/1‑148 (MQ=255)
                gcgcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCggg                                                                                                                                    <  1:2746844/148‑1 (MQ=255)
                 cgcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGc                                                                                                                                   <  1:2951660/148‑1 (MQ=255)
                 cgcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGc                                                                                                                                   <  1:1043188/148‑1 (MQ=255)
                   ccATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCG‑CCGGGccg                                                                                                                                 >  1:1903647/1‑146 (MQ=255)
                    cATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGGCC‑CCCGGCacg                                                                                                                                >  1:3223735/1‑146 (MQ=255)
                     aTCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGGCG‑CCGGGCCGGt                                                                                                                               >  1:1397539/1‑148 (MQ=255)
                      tCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCCGGGCGGGt                                                                                                                               >  1:1715094/1‑148 (MQ=255)
                         gACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCG‑CCGGGCAGGTTTcc                                                                                                                           >  2:344919/1‑148 (MQ=255)
                          aCCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGCCG‑CCGGGCCGGGTTCCa                                                                                                                          >  1:3103339/1‑148 (MQ=255)
                          aCCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTACGGCGGGCCG‑CCGGGCCGGTTTccc                                                                                                                          >  1:3102482/1‑147 (MQ=255)
                           ccGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAg                                                                                                                         >  2:3594482/1‑148 (MQ=255)
                             gggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGAc                                                                                                                       <  2:13882/148‑1 (MQ=255)
                               gggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTACAGACCa                                                                                                                     >  2:147461/1‑148 (MQ=255)
                                  cTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCt                                                                                                                  <  2:2419422/148‑1 (MQ=255)
                                   ttCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCtt                                                                                                                 <  2:2656810/148‑1 (MQ=255)
                                     cGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTc                                                                                                               <  2:1005644/148‑1 (MQ=255)
                                     cGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTc                                                                                                               <  1:459087/148‑1 (MQ=255)
                                     cGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTc                                                                                                               <  1:497347/148‑1 (MQ=255)
                                      gCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCa                                                                                                              <  2:1830969/148‑1 (MQ=255)
                                       ccGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCAt                                                                                                             <  1:382813/148‑1 (MQ=255)
                                        cGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCAtg                                                                                                            <  1:3487371/148‑1 (MQ=255)
                                          ggCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCAtgtg                                                                                                          <  2:1654768/148‑1 (MQ=255)
                                            cgcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCTGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTgag                                                                                                        <  2:1183202/148‑1 (MQ=255)
                                                  caTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCg                                                                                                  <  2:885071/148‑1 (MQ=255)
                                                    tGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGGCG‑CCGGGGCGGTTTGCAGACCCCCGTTACTGTGAGGTATCggg                                                                                                >  1:1493588/1‑147 (MQ=255)
                                                       cGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGAt                                                                                             <  1:3260156/148‑1 (MQ=255)
                                                        ggtCCCTTCCGGCAAATCACGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGAtt                                                                                            <  1:2878478/146‑1 (MQ=255)
                                                         ttCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGAttt                                                                                           <  1:2792883/148‑1 (MQ=255)
                                                          tCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCC‑CCCGGCCGGTTTACAGACCCCCTTTAAAGTTAGATAGCGGTGAtttt                                                                                          >  1:1982967/1‑148 (MQ=255)
                                                                ccGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGct                                                                                    <  1:645509/148‑1 (MQ=255)
                                                                ccGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGct                                                                                    <  1:1499118/148‑1 (MQ=255)
                                                                 cGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGctc                                                                                   <  2:3042870/148‑1 (MQ=255)
                                                                       aTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGGGCCG‑CCCGGGCGGTTTACAGTCCACCGTTCATGTGTGATAGCGGTGATTTTACCGCGCGGGTCg                                                                             >  2:1387044/1‑148 (MQ=255)
                                                                       aTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTGAGGTGAGATAGCGGTGATTTTACCGCTCTGGTCg                                                                             >  2:1124412/1‑148 (MQ=255)
                                                                       aTCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCG‑CCGGGCAGGGTTCCAGACCAGCATTCATGTGAGATAGCGGTGATTTTCCCGCTCGGGTCg                                                                             >  2:2138155/1‑148 (MQ=255)
                                                                         cAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGc                                                                           <  2:2082217/148‑1 (MQ=255)
                                                                            gCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGGTTTTCCCGCTCTGGTCGGCAcc                                                                        >  2:2964753/1‑148 (MQ=255)
                                                                             cGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCg                                                                       <  2:1194378/148‑1 (MQ=255)
                                                                               tGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGAt                                                                     <  2:287778/148‑1 (MQ=255)
                                                                                 gCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATAc                                                                   <  1:2818357/148‑1 (MQ=255)
                                                                                 gCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATAc                                                                   <  1:903737/148‑1 (MQ=255)
                                                                                   gAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGGGCCG‑CCCGGCAGGTTTCCATACCAGCTTTCAAGTGACATAGAGGTGAGATTCCCGCTCTGGTCTGCAACGATACGc                                                                 >  2:2780805/1‑148 (MQ=255)
                                                                                    aGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGCCG‑CCGGGCAGGTTTACAGAACAGCTTTCATGTGAGATACCGGTGATTTTACCCCTCAGGGCGGCACCGATAAGCa                                                                >  2:1731941/1‑148 (MQ=255)
                                                                                    aGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCCGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGcc                                                                >  2:2206482/1‑147 (MQ=255)
                                                                                    aGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGGTTTTCCCGCTCTGGTCGGCACCGATACGCa                                                                >  2:291698/1‑148 (MQ=255)
                                                                                    aGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCG‑CCCGGCAGGTTTCCAGACCACCATTCATGTGTGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCa                                                                >  2:3624137/1‑148 (MQ=255)
                                                                                    aGCCGCAGACCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCG‑CCGGGCAGGTTTACAGACCACCTTTCATGTGAGATAGCGGGGAATTTAACCCACTGGTCGGCACCCATAAGCa                                                                >  2:1504225/1‑148 (MQ=255)
                                                                                     gCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCaa                                                               <  2:2255831/148‑1 (MQ=255)
                                                                                     gCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCaa                                                               <  2:2526652/148‑1 (MQ=255)
                                                                                     gCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCaa                                                               <  1:3420216/148‑1 (MQ=255)
                                                                                     gCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACCGCGGGCCG‑CCGGGCCCGTTTCCAGACCAGCTTTCATGTGAGATATCGGTGATTTTCCCGCTCTGGTCGGCACCCAGACGCaa                                                               >  2:1073512/1‑148 (MQ=255)
                                                                                        gCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTg                                                            <  2:376230/148‑1 (MQ=255)
                                                                                         cAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGc                                                           <  2:714551/148‑1 (MQ=255)
                                                                                           ggCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCCGGTTTCCAGACCAGCTTTCCTGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTg                                                         >  2:2244148/1‑148 (MQ=255)
                                                                                            gCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCG‑CCCGGCCGGGTTGCAGACCACCATTCATGTGTGATAGCGGGGTTTTTTCCGCCGGGGTCGGGACCCCAAAGCAAGTGCTGa                                                        >  1:2135589/1‑148 (MQ=255)
                                                                                               ggTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAgg                                                     <  2:3249791/148‑1 (MQ=255)
                                                                                                gTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAggg                                                    <  2:3605580/148‑1 (MQ=255)
                                                                                                gTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAggg                                                    <  2:3493422/148‑1 (MQ=255)
                                                                                                 tATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTACAGACCAGCGTTCATGTGTGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCACTTGCGGAAGGGt                                                   >  2:2357428/1‑148 (MQ=255)
                                                                                                   tGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTgg                                                 <  1:1988182/148‑1 (MQ=255)
                                                                                                   tGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGTGATAGCGGGGTTTTTACCGCGCTGGGCGGCACCGATACGCAACTGGGGAAGGGTgg                                                 >  2:1708422/1‑148 (MQ=255)
                                                                                                   tGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCG‑CCGGGCAGGGTTACAGACCAGCGTTCATGGGAGATAGCGGTGTTTTTCCCGCTCGGGTCGGCACCGATAAGCACGTGGGGAAGGGTgg                                                 >  2:3602510/1‑148 (MQ=255)
                                                                                                         ttCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGGGCCG‑CCGGGCAGGTTTCCAGACCCCCGTTGATGTGAGGTAGCGGTGATTTTACCGCTCTGGTCGGCACCCCGAAGCCAGTGCGGAAGGGTgggggggg                                           >  2:1075285/1‑147 (MQ=255)
                                                                                                         ttCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCCCGTTTCCAGAACAGCATTCATGTGAGATATCGGTGATTTTACCGCGCTGGTCGGCACCCATAAGCAATTGCGGAAGGGTgggggggc                                           >  2:2661779/1‑148 (MQ=255)
                                                                                                         ttCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCCTGCCG‑CCCGGCAGGTTTCCAGACCAGCCTTCATTTTAGATAGCGGGGATTTTGCCGCTAGGGTCGGCACCGATACGCAAGTGCTGAAGGGTggcggggc                                           >  2:1510857/1‑148 (MQ=255)
                                                                                                          tCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGGGCCG‑CCCGGCAGGTTTCCAGACCAGCTTTCATGTGTGTTAGCGGGGATTTTACCGCGCTGGTCGGCACCGATACGCAACTGCGGAAGGGTggcgggggg                                          >  2:1428299/1‑146 (MQ=255)
                                                                                                                  gCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtgg                                  <  2:337414/148‑1 (MQ=255)
                                                                                                                   cGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggt                                 <  2:1374406/148‑1 (MQ=255)
                                                                                                                   cGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggt                                 <  1:1576352/148‑1 (MQ=255)
                                                                                                                   cGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggt                                 <  2:3271932/148‑1 (MQ=255)
                                                                                                                   cGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggt                                 <  1:2025543/148‑1 (MQ=255)
                                                                                                                   cGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggt                                 <  1:82957/148‑1 (MQ=255)
                                                                                                                     tAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGgtgt                               <  1:246569/148‑1 (MQ=255)
                                                                                                                      agagTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGtt                              <  1:3254532/148‑1 (MQ=255)
                                                                                                                              tCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGaa                      <  2:3103339/148‑1 (MQ=255)
                                                                                                                              tCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGaa                      <  2:3102482/148‑1 (MQ=255)
                                                                                                                               cGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAAt                     <  1:2687304/148‑1 (MQ=255)
                                                                                                                               cGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAAt                     <  2:3533715/148‑1 (MQ=255)
                                                                                                                                  tttGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATaga                  <  2:1561892/148‑1 (MQ=255)
                                                                                                                                  tttGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATaga                  <  2:1561946/148‑1 (MQ=255)
                                                                                                                                  tttGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATaga                  <  2:1232713/148‑1 (MQ=255)
                                                                                                                                     gCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAggg               <  2:3743833/148‑1 (MQ=255)
                                                                                                                                           ccgccgTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCgggg         <  2:1493588/148‑1 (MQ=255)
                                                                                                                                            cgccgTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCggggg        <  1:2328147/148‑1 (MQ=255)
                                                                                                                                              ccgTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTACCCCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGGGGTGTTATTGGGAATAGAGGGGCGGGGGAg      >  1:2509130/1‑148 (MQ=255)
                                                                                                                                               cgTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGc     <  2:3415503/148‑1 (MQ=255)
                                                                                                                                                gTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCa    >  1:1406522/1‑148 (MQ=255)
                                                                                                                                                 tttCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCAt   >  2:2317423/1‑148 (MQ=255)
                                                                                                                                                 tttCCGGCGTGCCG‑ACGGGCAGGTTTCCAGACCAGCATTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACAGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGTGGCGGGGGAGCAt   >  2:3235675/1‑148 (MQ=255)
                                                                                                                                                  ttCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATc  >  2:1267726/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCG‑CCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATC  >  CP009273/295549‑295842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A