Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,296,729 C→A 93.4% H431N (CAC→AAC)  oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,296,7290CA93.4% 648.5 / 35.8 229H431N (CAC→AAC) oppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (10/5);  new base A (123/91);  total (133/96)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 229 total aligned reads displayed.

AACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAA  >  CP009273/1296586‑1296873
                                                                                                                                               |                                                                                                                                                
aaCCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCa                                                                                                                                              <  1:1531793/148‑1 (MQ=255)
     ttGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTa                                                                                                                                         >  1:3081151/1‑148 (MQ=255)
      tGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTAc                                                                                                                                        >  1:822527/1‑148 (MQ=255)
         ccATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACttt                                                                                                                                     <  1:3227956/148‑1 (MQ=255)
               aCCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtgt                                                                                                                               >  1:738205/1‑148 (MQ=255)
                ccTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtgtg                                                                                                                              >  2:1355349/1‑148 (MQ=255)
                ccTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtgtg                                                                                                                              <  2:1676425/148‑1 (MQ=255)
                ccTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtgtg                                                                                                                              >  2:668235/1‑148 (MQ=255)
                ccTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtgtg                                                                                                                              <  1:3435617/148‑1 (MQ=255)
                 cTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTgg                                                                                                                             >  2:2569005/1‑148 (MQ=255)
                   gttgtATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGcc                                                                                                                           >  2:587076/1‑148 (MQ=255)
                    ttgtATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGccc                                                                                                                          >  2:329987/1‑148 (MQ=255)
                     tgtATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCg                                                                                                                         >  2:466118/1‑148 (MQ=255)
                      gtATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGt                                                                                                                        >  2:2371814/1‑148 (MQ=255)
                        ataACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGc                                                                                                                      >  2:2193234/1‑148 (MQ=255)
                         taACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCa                                                                                                                     >  1:276257/1‑148 (MQ=255)
                          aaCACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAg                                                                                                                    >  2:1767031/1‑148 (MQ=255)
                          aaCACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAg                                                                                                                    >  2:1405413/1‑148 (MQ=255)
                          aaCACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAg                                                                                                                    >  2:30003/1‑148 (MQ=255)
                          aaCACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCACGAGTGTAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAg                                                                                                                    >  1:2503627/1‑148 (MQ=255)
                           acacCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAgg                                                                                                                   <  2:1803714/148‑1 (MQ=255)
                            cacCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGc                                                                                                                  >  1:1874082/1‑148 (MQ=255)
                            cacCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGc                                                                                                                  <  1:1462832/148‑1 (MQ=255)
                            cacCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGc                                                                                                                  <  2:862647/148‑1 (MQ=255)
                              ccTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTg                                                                                                                >  1:2960500/1‑148 (MQ=255)
                               cTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTgg                                                                                                               >  1:2747731/1‑148 (MQ=255)
                               cTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCATGCAGGCTgg                                                                                                               >  1:706771/1‑148 (MQ=255)
                                tCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgt                                                                                                              >  2:2765487/1‑148 (MQ=255)
                                tCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgt                                                                                                              >  1:587330/1‑148 (MQ=255)
                                    aTCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGc                                                                                                          <  1:254342/148‑1 (MQ=255)
                                    aTCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGc                                                                                                          >  2:2016187/1‑148 (MQ=255)
                                     tCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCt                                                                                                         >  2:946747/1‑148 (MQ=255)
                                     tCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCt                                                                                                         >  2:1042674/1‑148 (MQ=255)
                                      cTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTg                                                                                                        >  2:3401941/1‑148 (MQ=255)
                                       tGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGa                                                                                                       <  2:2465898/148‑1 (MQ=255)
                                           tAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTAc                                                                                                   >  2:2776190/1‑148 (MQ=255)
                                              aaaGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAAc                                                                                                >  2:567547/1‑148 (MQ=255)
                                               aaGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACg                                                                                               >  2:3220681/1‑148 (MQ=255)
                                                 gCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAAACAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGaa                                                                                             >  2:1906991/1‑148 (MQ=255)
                                                 gCTGGCGAGTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGaa                                                                                             <  2:72245/148‑1 (MQ=255)
                                                  cTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAAc                                                                                            <  1:3427439/148‑1 (MQ=255)
                                                    ggCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCa                                                                                          >  2:2612340/1‑148 (MQ=255)
                                                     gCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCaa                                                                                         >  1:1721273/1‑148 (MQ=255)
                                                     gCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCaa                                                                                         <  2:2083036/148‑1 (MQ=255)
                                                     gCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCaa                                                                                         >  2:269376/1‑148 (MQ=255)
                                                         ttGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActtc                                                                                     >  1:1612642/1‑148 (MQ=255)
                                                         ttGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActtc                                                                                     <  1:1835252/148‑1 (MQ=255)
                                                          tgctgcCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActtcc                                                                                    >  2:1229424/1‑148 (MQ=255)
                                                           gctgcCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActtcct                                                                                   <  1:587076/148‑1 (MQ=255)
                                                            ctgcCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAacttactt                                                                                  <  2:3214027/148‑1 (MQ=255)
                                                             tgcCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActtccttc                                                                                 <  2:2596795/148‑1 (MQ=255)
                                                             tgcCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActtccttc                                                                                 <  1:1199241/148‑1 (MQ=255)
                                                               ccTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTtccttcct                                                                               >  2:1101027/1‑148 (MQ=255)
                                                                  ctTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGaa                                                                            >  1:3445870/1‑148 (MQ=255)
                                                                    tCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAaca                                                                          <  1:1511364/148‑1 (MQ=255)
                                                                      aTTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACAcc                                                                        >  1:1552249/1‑148 (MQ=255)
                                                                       ttGTGGAAGAAAAACATTGGTGTAAACGTAAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCa                                                                       >  1:1782684/1‑148 (MQ=255)
                                                                        tgtgGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCAt                                                                      >  1:1920039/1‑148 (MQ=255)
                                                                              agaaAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTc                                                                >  1:3329435/1‑148 (MQ=255)
                                                                                aaaaaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGa                                                              >  2:985458/1‑148 (MQ=255)
                                                                                aaaaaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGa                                                              >  2:625988/1‑148 (MQ=255)
                                                                                 aaaaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGaa                                                             <  2:35233/148‑1 (MQ=255)
                                                                                  aaaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAAc                                                            <  1:1883836/148‑1 (MQ=255)
                                                                                     cATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGc                                                         <  2:1680702/148‑1 (MQ=255)
                                                                                      aTTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCt                                                        <  2:2960500/148‑1 (MQ=255)
                                                                                       ttGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTc                                                       >  1:445874/1‑148 (MQ=255)
                                                                                       ttGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTc                                                       >  1:379877/1‑148 (MQ=255)
                                                                                        tGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCg                                                      >  1:383985/1‑148 (MQ=255)
                                                                                        tGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCg                                                      <  1:2929866/148‑1 (MQ=255)
                                                                                          gtgtAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGAt                                                    <  1:2557089/148‑1 (MQ=255)
                                                                                               aaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATa                                               >  1:2843977/1‑148 (MQ=255)
                                                                                               aaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATa                                               >  1:2069111/1‑148 (MQ=255)
                                                                                               aaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATa                                               >  1:2069192/1‑148 (MQ=255)
                                                                                                 cGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAcc                                             >  1:955687/1‑148 (MQ=255)
                                                                                                 cGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAcc                                             >  1:899463/1‑148 (MQ=255)
                                                                                                    cAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACcgcg                                          <  1:329987/148‑1 (MQ=255)
                                                                                                         tGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATta                                     >  2:1944795/1‑148 (MQ=255)
                                                                                                            ttAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATaa                                  >  1:3535125/1‑148 (MQ=255)
                                                                                                            ttAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGAAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATaa                                  >  2:1151587/1‑148 (MQ=255)
                                                                                                             tAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAag                                 <  1:1445671/148‑1 (MQ=255)
                                                                                                              aaCCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAaga                                <  1:1268460/148‑1 (MQ=255)
                                                                                                               aCCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAagag                               >  2:2425127/1‑148 (MQ=255)
                                                                                                                ccAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGc                              <  2:1874082/148‑1 (MQ=255)
                                                                                                                ccAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGc                              >  1:1264558/1‑148 (MQ=255)
                                                                                                                 cAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGcc                             >  1:2261175/1‑148 (MQ=255)
                                                                                                                 cAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGcc                             <  2:3445817/148‑1 (MQ=255)
                                                                                                                 cAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGcc                             <  2:3445893/148‑1 (MQ=255)
                                                                                                                   ggAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCg                           >  2:1596569/1‑148 (MQ=255)
                                                                                                                    gAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCgg                          >  1:1591320/1‑148 (MQ=255)
                                                                                                                     aGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGc                         <  2:2890351/148‑1 (MQ=255)
                                                                                                                      gTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGcc                        <  2:3128764/148‑1 (MQ=255)
                                                                                                                            aaCGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGAc                  <  1:2654977/148‑1 (MQ=255)
                                                                                                                             aCGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACa                 >  1:1748618/1‑148 (MQ=255)
                                                                                                                              cGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAg                <  2:1409642/148‑1 (MQ=255)
                                                                                                                                ttCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCa              <  2:2112743/148‑1 (MQ=255)
                                                                                                                                    tCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTAt          <  2:275820/148‑1 (MQ=255)
                                                                                                                                    tCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTAt          >  2:2353023/1‑148 (MQ=255)
                                                                                                                                     cGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATg         <  2:1545480/148‑1 (MQ=255)
                                                                                                                                     cGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATg         <  1:2360322/148‑1 (MQ=255)
                                                                                                                                          cccGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGa    <  1:109119/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
AACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAA  >  CP009273/1296586‑1296873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTG  >  CP009273/1296580‑1296878
                                                                                                                                                     |                                                                                                                                                     
CAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTC                                                                                                                                                       <  SRR19973066.1848748/150‑1 (MQ=60)
     AAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAG                                                                                                                                                  >  SRR19973066.1578790/1‑150 (MQ=60)
      AACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGG                                                                                                                                                 <  SRR19973066.1542694/150‑1 (MQ=60)
       ACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGG                                                                                                                                                <  SRR19973066.584296/150‑1 (MQ=60)
       ACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGG                                                                                                                                                <  SRR19973066.584148/150‑1 (MQ=60)
         CGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTA                                                                                                                                              >  SRR19973066.3101734/1‑150 (MQ=60)
          GTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTAC                                                                                                                                             >  SRR19973066.828534/1‑150 (MQ=60)
              ACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTT                                                                                                                                         <  SRR19973066.39110/150‑1 (MQ=60)
               CCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTG                                                                                                                                        <  SRR19973066.3249292/150‑1 (MQ=60)
                   CAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGT                                                                                                                                    >  SRR19973066.743684/1‑150 (MQ=60)
                   CAACCTGTTGTAGAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGT                                                                                                                                    <  SRR19973066.1271508/150‑1 (MQ=60)
                    AACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTG                                                                                                                                   <  SRR19973066.2784585/150‑1 (MQ=60)
                      CCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGC                                                                                                                                 <  SRR19973066.3458605/150‑1 (MQ=60)
                       CTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCC                                                                                                                                <  SRR19973066.1969183/150‑1 (MQ=60)
                         GTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCG                                                                                                                              <  SRR19973066.3329511/150‑1 (MQ=60)
                             TATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCA                                                                                                                          >  SRR19973066.278281/1‑150 (MQ=60)
                             TATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCA                                                                                                                          >  SRR19973066.277843/1‑150 (MQ=60)
                              ATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCACGAGTGTAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAG                                                                                                                         >  SRR19973066.2520679/1‑150 (MQ=60)
                                AACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGC                                                                                                                       >  SRR19973066.1887506/1‑150 (MQ=60)
                                AACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGC                                                                                                                       >  SRR19973066.1887460/1‑150 (MQ=60)
                                  CACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTG                                                                                                                     <  SRR19973066.1473172/150‑1 (MQ=60)
                                  CACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTG                                                                                                                     >  SRR19973066.2980108/1‑150 (MQ=60)
                                   ACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGG                                                                                                                    >  SRR19973066.2766219/1‑150 (MQ=60)
                                   ACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGG                                                                                                                    >  SRR19973066.2976816/1‑150 (MQ=60)
                                   ACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCATGCAGGCTGG                                                                                                                    >  SRR19973066.711997/1‑150 (MQ=60)
                                   ACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCATGCAGGCTGG                                                                                                                    >  SRR19973066.712069/1‑150 (MQ=60)
                                    CCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGT                                                                                                                   >  SRR19973066.591426/1‑150 (MQ=60)
                                    CCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGT                                                                                                                   <  SRR19973066.1716281/150‑1 (MQ=60)
                                     CTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTG                                                                                                                  <  SRR19973066.1297564/150‑1 (MQ=60)
                                          ATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTG                                                                                                             <  SRR19973066.256276/150‑1 (MQ=60)
                                                ATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACA                                                                                                       <  SRR19973066.1637773/150‑1 (MQ=60)
                                                   AAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACG                                                                                                    >  SRR19973066.2749258/1‑150 (MQ=60)
                                                        CTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCA                                                                                               <  SRR19973066.3450362/150‑1 (MQ=60)
                                                         TGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAA                                                                                              >  SRR19973066.1733734/1‑150 (MQ=60)
                                                             GATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTC                                                                                          >  SRR19973066.1624337/1‑150 (MQ=60)
                                                              ATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCC                                                                                         >  SRR19973066.747758/1‑150 (MQ=60)
                                                               TTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCT                                                                                        <  SRR19973066.1848361/150‑1 (MQ=60)
                                                               TTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCT                                                                                        <  SRR19973066.1851724/150‑1 (MQ=60)
                                                                 GCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTC                                                                                      <  SRR19973066.591170/150‑1 (MQ=60)
                                                                 GCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTC                                                                                      <  SRR19973066.591187/150‑1 (MQ=60)
                                                                   TGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCT                                                                                    <  SRR19973066.1207603/150‑1 (MQ=60)
                                                                      CTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAA                                                                                 >  SRR19973066.3468940/1‑150 (MQ=60)
                                                                      CTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAA                                                                                 >  SRR19973066.3468864/1‑150 (MQ=60)
                                                                      CTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAA                                                                                 >  SRR19973066.3468917/1‑150 (MQ=60)
                                                                        CTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACA                                                                               >  SRR19973066.3221022/1‑150 (MQ=60)
                                                                          TCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACC                                                                             <  SRR19973066.1521945/150‑1 (MQ=60)
                                                                          TCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACC                                                                             >  SRR19973066.1563385/1‑150 (MQ=60)
                                                                           CATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCA                                                                            >  SRR19973066.2909679/1‑150 (MQ=60)
                                                                           CATTGTGGAAGAAAAACATTGGTGTAAACGTAAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCA                                                                            >  SRR19973066.1795412/1‑150 (MQ=60)
                                                                           CATTGTGGAAGAAAAACATTGGTGTAAACGTAAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCA                                                                            >  SRR19973066.1795415/1‑150 (MQ=60)
                                                                            ATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCAT                                                                           >  SRR19973066.1933697/1‑150 (MQ=60)
                                                                                TGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTT                                                                       <  SRR19973066.89281/150‑1 (MQ=60)
                                                                                TGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTT                                                                       <  SRR19973066.2637909/150‑1 (MQ=60)
                                                                                TGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTT                                                                       >  SRR19973066.3195338/1‑150 (MQ=60)
                                                                                  GAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTC                                                                     >  SRR19973066.2646864/1‑150 (MQ=60)
                                                                                  GAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTC                                                                     >  SRR19973066.3351557/1‑150 (MQ=60)
                                                                                  GAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTATC                                                                     >  SRR19973066.1172557/1‑150 (MQ=60)
                                                                                     GAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAA                                                                  <  SRR19973066.1528517/150‑1 (MQ=60)
                                                                                      AAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAAC                                                                 <  SRR19973066.918503/150‑1 (MQ=60)
                                                                                      AAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAAC                                                                 >  SRR19973066.183395/1‑150 (MQ=60)
                                                                                       AAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACA                                                                >  SRR19973066.3488450/1‑150 (MQ=60)
                                                                                       AAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTAGATGTGGCCCGTGCAGGCTGGTGTGATGACTACAACGAACCAACTTCCTTCCTGAACACCAAGCTTTAGAACA                                                                >  SRR19973066.1847296/1‑150 (MQ=60)
                                                                                        AAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAG                                                               <  SRR19973066.1619835/150‑1 (MQ=60)
                                                                                        AAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAG                                                               <  SRR19973066.1897276/150‑1 (MQ=60)
                                                                                        AAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTACTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAG                                                               <  SRR19973066.1523705/150‑1 (MQ=60)
                                                                                           CATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTC                                                            <  SRR19973066.2794877/150‑1 (MQ=60)
                                                                                           CATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTC                                                            <  SRR19973066.1001458/150‑1 (MQ=60)
                                                                                           CATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTC                                                            >  SRR19973066.382866/1‑150 (MQ=60)
                                                                                           CATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTC                                                            >  SRR19973066.382775/1‑150 (MQ=60)
                                                                                           CATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTC                                                            >  SRR19973066.449306/1‑150 (MQ=60)
                                                                                           CATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTC                                                            >  SRR19973066.382904/1‑150 (MQ=60)
                                                                                            ATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCG                                                           >  SRR19973066.387015/1‑150 (MQ=60)
                                                                                              TGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGAT                                                         <  SRR19973066.2949372/150‑1 (MQ=60)
                                                                                                GTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGA                                                       <  SRR19973066.1746477/150‑1 (MQ=60)
                                                                                                GTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGA                                                       <  SRR19973066.2574450/150‑1 (MQ=60)
                                                                                                   TAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATA                                                    >  SRR19973066.2083515/1‑150 (MQ=60)
                                                                                                   TAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATA                                                    <  SRR19973066.2566236/150‑1 (MQ=60)
                                                                                                   TAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATA                                                    >  SRR19973066.2083442/1‑150 (MQ=60)
                                                                                                   TAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATA                                                    >  SRR19973066.2083434/1‑150 (MQ=60)
                                                                                                   TAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATA                                                    >  SRR19973066.2863091/1‑150 (MQ=60)
                                                                                                    AAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAC                                                   <  SRR19973066.241322/150‑1 (MQ=60)
                                                                                                     AACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACC                                                  >  SRR19973066.707496/1‑150 (MQ=60)
                                                                                                     AACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACC                                                  >  SRR19973066.905811/1‑150 (MQ=60)
                                                                                                     AACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACC                                                  >  SRR19973066.962678/1‑150 (MQ=60)
                                                                                                     AACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACC                                                  <  SRR19973066.1001792/150‑1 (MQ=60)
                                                                                                          CAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCA                                             <  SRR19973066.332501/150‑1 (MQ=60)
                                                                                                            AACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATT                                           >  SRR19973066.2543561/1‑150 (MQ=60)
                                                                                                               TGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATA                                        >  SRR19973066.3451297/1‑150 (MQ=60)
                                                                                                                GGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAA                                       >  SRR19973066.3558631/1‑150 (MQ=60)
                                                                                                                 GTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAAAACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAG                                      >  SRR19973066.726605/1‑150 (MQ=60)
                                                                                                                   TAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAG                                    <  SRR19973066.1455952/150‑1 (MQ=60)
                                                                                                                   TAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAG                                    >  SRR19973066.1285762/1‑150 (MQ=60)
                                                                                                                   TAACCAGGAGTGGAAAACGGTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAG                                    <  SRR19973066.1455957/150‑1 (MQ=60)
                                                                                                                    AACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGC                                   >  SRR19973066.3061538/1‑150 (MQ=60)
                                                                                                                    AACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGC                                   >  SRR19973066.1273686/1‑150 (MQ=60)
                                                                                                                    AACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGC                                   <  SRR19973066.1277619/150‑1 (MQ=60)
                                                                                                                    AACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGC                                   <  SRR19973066.1274709/150‑1 (MQ=60)
                                                                                                                     ACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATCCCGCGCATTATAAGAGCC                                  >  SRR19973066.2276751/1‑150 (MQ=60)
                                                                                                                     ACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCC                                  >  SRR19973066.2276745/1‑150 (MQ=60)
                                                                                                                        AGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCGACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGG                               >  SRR19973066.3268713/1‑150 (MQ=60)
                                                                                                                        AGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCGACTTCCTTCCTGAACACCATGCTTTCGAACAGCGCGATGAATACCGCGCATTATAAGAGCCCGG                               >  SRR19973066.1486573/1‑150 (MQ=60)
                                                                                                                        AGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGG                               >  SRR19973066.219830/1‑150 (MQ=60)
                                                                                                                        AGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGG                               >  SRR19973066.1602837/1‑150 (MQ=60)
                                                                                                                        AGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCATGAACACCATGCTTTCGAACAGCTCGATGAATACCTCGCAATATAAGAGCCCGG                               >  SRR19973066.2032077/1‑150 (MQ=60)
                                                                                                                             TGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTT                          <  SRR19973066.155485/150‑1 (MQ=60)
                                                                                                                                 AAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACA                      >  SRR19973066.1761200/1‑150 (MQ=60)
                                                                                                                                  AACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAG                     <  SRR19973066.2672785/150‑1 (MQ=60)
                                                                                                                                       TCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTA                <  SRR19973066.3424653/150‑1 (MQ=60)
                                                                                                                                        CCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTAT               >  SRR19973066.2284660/1‑150 (MQ=60)
                                                                                                                                         CTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATG              >  SRR19973066.3363657/1‑150 (MQ=60)
                                                                                                                                           CGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGC            <  SRR19973066.2376323/150‑1 (MQ=60)
                                                                                                                                             ACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGG          >  SRR19973066.1909481/1‑150 (MQ=60)
                                                                                                                                                CCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAA       <  SRR19973066.110235/150‑1 (MQ=60)
                                                                                                                                                  CGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACG     >  SRR19973066.632745/1‑150 (MQ=60)
                                                                                                                                                   GTAACCAGGGTACGTTTGATGTGGCCCGTGCAGGCTGGTTTGCTGACTACAACGAACCAACTTCCTTCAAGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTATGACAGCATTATGTCGGAAACGC    >  SRR19973066.3283123/1‑150 (MQ=60)
                                                                                                                                                    TAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCT   >  SRR19973066.1783896/1‑150 (MQ=60)
                                                                                                                                                     AACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTG  <  SRR19973066.723365/150‑1 (MQ=60)
                                                                                                                                                     AACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTG  <  SRR19973066.622063/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTG  >  CP009273/1296580‑1296878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑