Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,296,729 C→A 100% H431N (CAC→AAC)  oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,296,7290CA97.8% 704.0 / 5.6 222H431N (CAC→AAC) oppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (116/101);  total (118/104)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 222 total aligned reads displayed.

CAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAA  >  CP009273/1296584‑1296873
                                                                                                                                                 |                                                                                                                                                
cAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAAc                                                                                                                                                >  2:3297257/1‑148 (MQ=255)
 aaaCCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAAcc                                                                                                                                               >  2:1028141/1‑148 (MQ=255)
   aCCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAg                                                                                                                                             >  2:3051303/1‑148 (MQ=255)
    ccGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAgg                                                                                                                                            <  2:1347269/148‑1 (MQ=255)
      gTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGt                                                                                                                                          <  1:2336536/148‑1 (MQ=255)
          aCCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACtt                                                                                                                                      >  1:2997361/1‑148 (MQ=255)
          aCCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACtt                                                                                                                                      >  1:2995720/1‑148 (MQ=255)
             aTCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTg                                                                                                                                   <  2:1414713/148‑1 (MQ=255)
               cAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAt                                                                                                                                 >  1:3155862/1‑148 (MQ=255)
               cAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAt                                                                                                                                 >  1:1060970/1‑148 (MQ=255)
                aaCCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtg                                                                                                                                >  1:1834657/1‑148 (MQ=255)
                  ccTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtgtg                                                                                                                              >  1:1723399/1‑148 (MQ=255)
                  ccTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtgtg                                                                                                                              >  2:1050806/1‑148 (MQ=255)
                  ccTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtgtg                                                                                                                              >  1:3460257/1‑148 (MQ=255)
                     gttgtATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGcc                                                                                                                           >  2:2927345/1‑148 (MQ=255)
                     gttgtATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGcc                                                                                                                           >  2:1363725/1‑148 (MQ=255)
                         tataACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGGAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTg                                                                                                                       >  2:1686441/1‑148 (MQ=255)
                         tataACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTg                                                                                                                       <  2:391974/148‑1 (MQ=255)
                         tataACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTg                                                                                                                       <  1:453789/148‑1 (MQ=255)
                           taACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCa                                                                                                                     <  2:1496807/148‑1 (MQ=255)
                            aaCACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAg                                                                                                                    >  1:2786332/1‑148 (MQ=255)
                             acacCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAgg                                                                                                                   >  2:2541051/1‑148 (MQ=255)
                             acacCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAgg                                                                                                                   >  1:1117154/1‑148 (MQ=255)
                              cacCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGc                                                                                                                  <  2:3441784/148‑1 (MQ=255)
                              cacCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGc                                                                                                                  <  1:1413572/148‑1 (MQ=255)
                               acCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCt                                                                                                                 <  1:1990318/148‑1 (MQ=255)
                                  tCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgt                                                                                                              >  2:2149951/1‑148 (MQ=255)
                                  tCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgt                                                                                                              >  1:317669/1‑148 (MQ=255)
                                   ccGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgtg                                                                                                             <  1:3072111/148‑1 (MQ=255)
                                     gATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGtgtg                                                                                                           <  1:1278138/148‑1 (MQ=255)
                                        cTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTCCTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTg                                                                                                        >  2:3284135/1‑148 (MQ=255)
                                          gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGAc                                                                                                      >  2:3220412/1‑148 (MQ=255)
                                          gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGAc                                                                                                      >  1:2865643/1‑148 (MQ=255)
                                          gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGAc                                                                                                      >  1:1107189/1‑148 (MQ=255)
                                           cATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACt                                                                                                     >  1:1795658/1‑148 (MQ=255)
                                             tAAAAAGCTGGCGAGTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTAc                                                                                                   <  2:415547/148‑1 (MQ=255)
                                              aaaaaGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACa                                                                                                  <  1:168040/148‑1 (MQ=255)
                                                 aaGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACg                                                                                               >  1:792160/1‑148 (MQ=255)
                                                  aGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGa                                                                                              <  1:1594789/148‑1 (MQ=255)
                                                  aGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGa                                                                                              <  1:1660400/148‑1 (MQ=255)
                                                   gCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGaa                                                                                             <  2:15446/148‑1 (MQ=255)
                                                          aTTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActt                                                                                      >  2:367289/1‑148 (MQ=255)
                                                               tgcCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActtccttc                                                                                 <  2:1480692/148‑1 (MQ=255)
                                                                   tctTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGa                                                                             <  1:632872/148‑1 (MQ=255)
                                                                    ctTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGaa                                                                            <  2:3501514/148‑1 (MQ=255)
                                                                      tCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAaca                                                                          <  2:229432/148‑1 (MQ=255)
                                                                        aTTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACAcc                                                                        <  1:371437/148‑1 (MQ=255)
                                                                        aTTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACAcc                                                                        >  1:934735/1‑148 (MQ=255)
                                                                          tgtgGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCAt                                                                      >  2:24739/1‑148 (MQ=255)
                                                                            tgGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGc                                                                    >  1:1932511/1‑148 (MQ=255)
                                                                            tgGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGc                                                                    <  1:613130/148‑1 (MQ=255)
                                                                            tgGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGc                                                                    <  1:3425106/148‑1 (MQ=255)
                                                                            tgGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGc                                                                    >  1:3415751/1‑148 (MQ=255)
                                                                            tgGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGc                                                                    <  1:2202627/148‑1 (MQ=255)
                                                                             ggAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCt                                                                   >  2:3358646/1‑148 (MQ=255)
                                                                              gaagaaAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCtt                                                                  >  2:924595/1‑148 (MQ=255)
                                                                              gaagaaAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCtt                                                                  >  1:2448289/1‑148 (MQ=255)
                                                                                 gaaAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCg                                                               >  1:2121783/1‑148 (MQ=255)
                                                                                   aaaaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGaa                                                             <  1:392101/148‑1 (MQ=255)
                                                                                   aaaaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGGTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGaa                                                             <  1:3506281/148‑1 (MQ=255)
                                                                                       cATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGc                                                         >  2:2234390/1‑148 (MQ=255)
                                                                                        aTTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCt                                                        <  1:2621025/148‑1 (MQ=255)
                                                                                        aTTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCt                                                        >  2:2395308/1‑148 (MQ=255)
                                                                                             tgtAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATg                                                   >  2:1813083/1‑148 (MQ=255)
                                                                                               tAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGaa                                                 >  1:417056/1‑148 (MQ=255)
                                                                                               tAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGaa                                                 >  1:415554/1‑148 (MQ=255)
                                                                                               tAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGaa                                                 <  1:1693403/148‑1 (MQ=255)
                                                                                                aaaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAAt                                                <  1:3489845/148‑1 (MQ=255)
                                                                                                   cGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAcc                                             >  2:1165893/1‑148 (MQ=255)
                                                                                                     tCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGACTACcgc                                           >  2:924269/1‑148 (MQ=255)
                                                                                                      cAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACcgcg                                          >  2:3223896/1‑148 (MQ=255)
                                                                                                      cAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACcgcg                                          >  2:3279607/1‑148 (MQ=255)
                                                                                                       aaaCTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCgcgc                                         <  1:1160524/148‑1 (MQ=255)
                                                                                                       aaaCTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCgcgc                                         >  2:723221/1‑148 (MQ=255)
                                                                                                       aaaCTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCgcgc                                         <  1:814140/148‑1 (MQ=255)
                                                                                                         aCTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGATTACCGCGCAt                                       <  2:1723448/148‑1 (MQ=255)
                                                                                                           tGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATta                                     >  1:1723855/1‑148 (MQ=255)
                                                                                                            ggTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATtat                                    >  1:778411/1‑148 (MQ=255)
                                                                                                             gTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATtata                                   <  1:950162/148‑1 (MQ=255)
                                                                                                              ttAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATaa                                  <  1:51713/148‑1 (MQ=255)
                                                                                                                 aCCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAagag                               <  2:710000/148‑1 (MQ=255)
                                                                                                                  ccAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGc                              <  1:2745641/148‑1 (MQ=255)
                                                                                                                  ccAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGc                              <  2:1095804/148‑1 (MQ=255)
                                                                                                                   cAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGcc                             <  1:3220412/148‑1 (MQ=255)
                                                                                                                   cAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGcc                             <  1:2090009/148‑1 (MQ=255)
                                                                                                                       aGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGc                         <  2:1457430/148‑1 (MQ=255)
                                                                                                                        gTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGcc                        >  2:2217185/1‑148 (MQ=255)
                                                                                                                         tGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCt                       >  1:201468/1‑148 (MQ=255)
                                                                                                                         tGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCt                       <  1:1466348/148‑1 (MQ=255)
                                                                                                                          ggAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCtt                      <  1:825042/148‑1 (MQ=255)
                                                                                                                              aaCGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGAc                  <  2:2348594/148‑1 (MQ=255)
                                                                                                                                 gTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGc               <  1:2171130/148‑1 (MQ=255)
                                                                                                                                    ccTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCAtt            >  2:1863572/1‑148 (MQ=255)
                                                                                                                                     cTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTa           >  2:463511/1‑148 (MQ=255)
                                                                                                                                        gACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATgg        <  1:3366519/148‑1 (MQ=255)
                                                                                                                                           acCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCgg     >  2:2805389/1‑148 (MQ=255)
                                                                                                                                             ccGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGaa   <  2:3165144/148‑1 (MQ=255)
                                                                                                                                             ccGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGaa   <  1:1578571/148‑1 (MQ=255)
                                                                                                                                              cGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGaaa  >  1:3178732/1‑148 (MQ=255)
                                                                                                                                              cGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGaaa  <  2:1208911/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAA  >  CP009273/1296584‑1296873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTG  >  CP009273/1296581‑1296878
                                                                                                                                                    |                                                                                                                                                     
AGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAA                                                                                                                                                      >  SRR19973065.1274850/1‑150 (MQ=60)
    AAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAG                                                                                                                                                  >  SRR19973065.445756/1‑150 (MQ=60)
         GTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTAC                                                                                                                                             <  SRR19973065.2351124/150‑1 (MQ=60)
           TGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTT                                                                                                                                           >  SRR19973065.2146719/1‑150 (MQ=60)
           TGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTT                                                                                                                                           >  SRR19973065.3014064/1‑150 (MQ=60)
           TGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTT                                                                                                                                           >  SRR19973065.3015674/1‑150 (MQ=60)
           TGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTT                                                                                                                                           >  SRR19973065.3015721/1‑150 (MQ=60)
                ATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAT                                                                                                                                      >  SRR19973065.1068003/1‑150 (MQ=60)
                ATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAT                                                                                                                                      >  SRR19973065.3175001/1‑150 (MQ=60)
                 TCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATG                                                                                                                                     >  SRR19973065.1846754/1‑150 (MQ=60)
                 TCAACCTGTTGTAGAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATG                                                                                                                                     <  SRR19973065.342138/150‑1 (MQ=60)
                   AACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTG                                                                                                                                   >  SRR19973065.1734889/1‑150 (MQ=60)
                   AACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTG                                                                                                                                   >  SRR19973065.1734840/1‑150 (MQ=60)
                   AACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTG                                                                                                                                   >  SRR19973065.3481335/1‑150 (MQ=60)
                      CTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCC                                                                                                                                <  SRR19973065.3396654/150‑1 (MQ=60)
                      CTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCC                                                                                                                                >  SRR19973065.2599714/1‑150 (MQ=60)
                      CTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCC                                                                                                                                >  SRR19973065.829220/1‑150 (MQ=60)
                            TATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCA                                                                                                                          <  SRR19973065.456930/150‑1 (MQ=60)
                             ATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAG                                                                                                                         >  SRR19973065.2803611/1‑150 (MQ=60)
                              TAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGG                                                                                                                        >  SRR19973065.1124447/1‑150 (MQ=60)
                                 CACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTG                                                                                                                     <  SRR19973065.1422810/150‑1 (MQ=60)
                                  ACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGG                                                                                                                    <  SRR19973065.2003228/150‑1 (MQ=60)
                                   CCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGT                                                                                                                   >  SRR19973065.319850/1‑150 (MQ=60)
                                      CCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTG                                                                                                                <  SRR19973065.3210702/150‑1 (MQ=60)
                                      CCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTG                                                                                                                <  SRR19973065.3090887/150‑1 (MQ=60)
                                        GATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCT                                                                                                              <  SRR19973065.1286577/150‑1 (MQ=60)
                                           CTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGAC                                                                                                           >  SRR19973065.2883229/1‑150 (MQ=60)
                                           CTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGAC                                                                                                           >  SRR19973065.1114443/1‑150 (MQ=60)
                                           CTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGAC                                                                                                           >  SRR19973065.668760/1‑150 (MQ=60)
                                           CTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGAC                                                                                                           >  SRR19973065.668703/1‑150 (MQ=60)
                                            TGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACT                                                                                                          >  SRR19973065.1807444/1‑150 (MQ=60)
                                              CATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTAC                                                                                                        >  SRR19973065.3524321/1‑150 (MQ=60)
                                                 AAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAAC                                                                                                     <  SRR19973065.169402/150‑1 (MQ=60)
                                                  AAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACG                                                                                                    >  SRR19973065.797405/1‑150 (MQ=60)
                                                     AGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAAC                                                                                                 >  SRR19973065.3123548/1‑150 (MQ=60)
                                                     AGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAAC                                                                                                 <  SRR19973065.1605445/150‑1 (MQ=60)
                                                     AGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAAC                                                                                                 <  SRR19973065.1671522/150‑1 (MQ=60)
                                                     AGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAAC                                                                                                 >  SRR19973065.1661549/1‑150 (MQ=60)
                                                       CTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCA                                                                                               <  SRR19973065.233758/150‑1 (MQ=60)
                                                             ATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCC                                                                                         <  SRR19973065.169131/150‑1 (MQ=60)
                                                             ATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCC                                                                                         <  SRR19973065.1884164/150‑1 (MQ=60)
                                                             ATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCC                                                                                         <  SRR19973065.1951940/150‑1 (MQ=60)
                                                              TTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCT                                                                                        <  SRR19973065.956388/150‑1 (MQ=60)
                                                                      TCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAAC                                                                                <  SRR19973065.636994/150‑1 (MQ=60)
                                                                         TCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACC                                                                             >  SRR19973065.941021/1‑150 (MQ=60)
                                                                         TCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACC                                                                             >  SRR19973065.1119482/1‑150 (MQ=60)
                                                                         TCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACC                                                                             >  SRR19973065.940908/1‑150 (MQ=60)
                                                                          CATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGCTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCA                                                                            >  SRR19973065.430779/1‑150 (MQ=60)
                                                                           ATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCAT                                                                           <  SRR19973065.374037/150‑1 (MQ=60)
                                                                             TGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGC                                                                         >  SRR19973065.3437210/1‑150 (MQ=60)
                                                                             TGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGC                                                                         >  SRR19973065.3436624/1‑150 (MQ=60)
                                                                             TGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGC                                                                         >  SRR19973065.1945163/1‑150 (MQ=60)
                                                                               TGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTT                                                                       >  SRR19973065.1192542/1‑150 (MQ=60)
                                                                               TGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTT                                                                       <  SRR19973065.617738/150‑1 (MQ=60)
                                                                               TGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTT                                                                       >  SRR19973065.2463717/1‑150 (MQ=60)
                                                                               TGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTT                                                                       <  SRR19973065.2216726/150‑1 (MQ=60)
                                                                               TGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTT                                                                       <  SRR19973065.3446011/150‑1 (MQ=60)
                                                                               TGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTT                                                                       <  SRR19973065.617051/150‑1 (MQ=60)
                                                                                 GTAGAAAAACATTGGTGTATACGTCAAACTGGTTAACCTTGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTC                                                                     <  SRR19973065.1304606/150‑1 (MQ=60)
                                                                                 GAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTC                                                                     >  SRR19973065.1401747/1‑150 (MQ=60)
                                                                                 GAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTC                                                                     >  SRR19973065.3155453/1‑150 (MQ=60)
                                                                                  AAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCATGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGATGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCG                                                                    <  SRR19973065.1143290/150‑1 (MQ=60)
                                                                                  AAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCG                                                                    >  SRR19973065.2135444/1‑150 (MQ=60)
                                                                                      AAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACA                                                                <  SRR19973065.366047/150‑1 (MQ=60)
                                                                                      AAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACA                                                                <  SRR19973065.394838/150‑1 (MQ=60)
                                                                                      AAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACA                                                                <  SRR19973065.2875782/150‑1 (MQ=60)
                                                                                      AAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGGTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACA                                                                <  SRR19973065.3527564/150‑1 (MQ=60)
                                                                                         ACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCT                                                             <  SRR19973065.3083777/150‑1 (MQ=60)
                                                                                         ACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCT                                                             <  SRR19973065.211239/150‑1 (MQ=60)
                                                                                          CATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTC                                                            <  SRR19973065.1602725/150‑1 (MQ=60)
                                                                                          CATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTC                                                            <  SRR19973065.2087436/150‑1 (MQ=60)
                                                                                           ATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCG                                                           >  SRR19973065.1431925/1‑150 (MQ=60)
                                                                                           ATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCG                                                           <  SRR19973065.2637248/150‑1 (MQ=60)
                                                                                                TGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAA                                                      >  SRR19973065.419974/1‑150 (MQ=60)
                                                                                                TGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAA                                                      >  SRR19973065.418466/1‑150 (MQ=60)
                                                                                                TGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAA                                                      >  SRR19973065.418467/1‑150 (MQ=60)
                                                                                                  TAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATA                                                    <  SRR19973065.1704712/150‑1 (MQ=60)
                                                                                                   AAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAC                                                   <  SRR19973065.3511069/150‑1 (MQ=60)
                                                                                                   AAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAC                                                   <  SRR19973065.3511106/150‑1 (MQ=60)
                                                                                                    AACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACC                                                  <  SRR19973065.2373320/150‑1 (MQ=60)
                                                                                                     ACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCG                                                 <  SRR19973065.377699/150‑1 (MQ=60)
                                                                                                      CGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGC                                                >  SRR19973065.2229265/1‑150 (MQ=60)
                                                                                                          AAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCAT                                            <  SRR19973065.2928934/150‑1 (MQ=60)
                                                                                                          AAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCAT                                            >  SRR19973065.3184315/1‑150 (MQ=60)
                                                                                                          AAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCAT                                            <  SRR19973065.819498/150‑1 (MQ=60)
                                                                                                          AAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCAT                                            <  SRR19973065.819506/150‑1 (MQ=60)
                                                                                                          AAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCAT                                            <  SRR19973065.1167953/150‑1 (MQ=60)
                                                                                                            ACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTA                                          >  SRR19973065.1678697/1‑150 (MQ=60)
                                                                                                            ACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTA                                          >  SRR19973065.1735296/1‑150 (MQ=60)
                                                                                                            ACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTA                                          >  SRR19973065.1735297/1‑150 (MQ=60)
                                                                                                            ACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTA                                          >  SRR19973065.3475429/1‑150 (MQ=60)
                                                                                                             CTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTAT                                         >  SRR19973065.783583/1‑150 (MQ=60)
                                                                                                              TGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATA                                        >  SRR19973065.149918/1‑150 (MQ=60)
                                                                                                               GGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAA                                       <  SRR19973065.2195472/150‑1 (MQ=60)
                                                                                                               GGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAA                                       >  SRR19973065.2811000/1‑150 (MQ=60)
                                                                                                                GTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAG                                      <  SRR19973065.956440/150‑1 (MQ=60)
                                                                                                                 TTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGA                                     <  SRR19973065.52282/150‑1 (MQ=60)
                                                                                                                    ACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCC                                  >  SRR19973065.896996/1‑150 (MQ=60)
                                                                                                                    ACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCC                                  >  SRR19973065.2038084/1‑150 (MQ=60)
                                                                                                                     CCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCC                                 <  SRR19973065.2762724/150‑1 (MQ=60)
                                                                                                                      CAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCG                                <  SRR19973065.3239768/150‑1 (MQ=60)
                                                                                                                      CAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCG                                <  SRR19973065.1758368/150‑1 (MQ=60)
                                                                                                                      CAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCG                                <  SRR19973065.2103421/150‑1 (MQ=60)
                                                                                                                       AGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGG                               >  SRR19973065.3497200/1‑150 (MQ=60)
                                                                                                                         GAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCC                             >  SRR19973065.787853/1‑150 (MQ=60)
                                                                                                                          AGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCT                            >  SRR19973065.202688/1‑150 (MQ=60)
                                                                                                                          AGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCT                            >  SRR19973065.203024/1‑150 (MQ=60)
                                                                                                                           GTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTT                           <  SRR19973065.1004780/150‑1 (MQ=60)
                                                                                                                            TGGGAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTT                          <  SRR19973065.1697711/150‑1 (MQ=60)
                                                                                                                            TGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTT                          <  SRR19973065.401879/150‑1 (MQ=60)
                                                                                                                            TGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTT                          <  SRR19973065.330739/150‑1 (MQ=60)
                                                                                                                            TGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTT                          <  SRR19973065.1475756/150‑1 (MQ=60)
                                                                                                                             GGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTG                         <  SRR19973065.1825017/150‑1 (MQ=60)
                                                                                                                             GGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTG                         <  SRR19973065.830453/150‑1 (MQ=60)
                                                                                                                                AAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACA                      >  SRR19973065.1732914/1‑150 (MQ=60)
                                                                                                                                    GTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCAT                  <  SRR19973065.2185064/150‑1 (MQ=60)
                                                                                                                                       CCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTAT               >  SRR19973065.1110269/1‑150 (MQ=60)
                                                                                                                                       CCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTAT               >  SRR19973065.519621/1‑150 (MQ=60)
                                                                                                                                        CTCGACACCCGTAACCAGGGTACTTTTGATGTGTCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGGATTATAAGAGCCCGGCCTTTGACAGCATTATG              <  SRR19973065.2773033/150‑1 (MQ=60)
                                                                                                                                           GACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCG           <  SRR19973065.3387105/150‑1 (MQ=60)
                                                                                                                                            ACACCCGTAACCAGGGTCCTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGG          >  SRR19973065.542416/1‑150 (MQ=60)
                                                                                                                                               CCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAA       >  SRR19973065.3197734/1‑150 (MQ=60)
                                                                                                                                               CCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAA       >  SRR19973065.3197942/1‑150 (MQ=60)
                                                                                                                                                CCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAAC      <  SRR19973065.1589092/150‑1 (MQ=60)
                                                                                                                                                 CGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACG     <  SRR19973065.2693473/150‑1 (MQ=60)
                                                                                                                                                  GTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGC    >  SRR19973065.3378610/1‑150 (MQ=60)
                                                                                                                                                   TAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCT   <  SRR19973065.3124647/150‑1 (MQ=60)
                                                                                                                                                   TAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCT   <  SRR19973065.1811821/150‑1 (MQ=60)
                                                                                                                                                    AACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGTCGGAAACGCTG  >  SRR19973065.3151241/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
AGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTG  >  CP009273/1296581‑1296878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑