Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F488 I0 R1	1275	300.1	10328444	91.0%	9398884	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,296,729	C→A	100%	H431N (CAC→AAC)	oppA →	oligopeptide transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,296,729	0	C	A	99.3%	918.1 / ‑3.0	278	H431N (CAC→AAC)	oppA	oligopeptide transporter subunit
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (130/146); total (131/147)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 278 total aligned reads displayed.

ACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACG  >  CP009273/1296583‑1296875

aCAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAGACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAAcc                                                                                                                                                 <  1:3924577/150‑1 (MQ=255)

aCAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAAcc                                                                                                                                                 <  2:4594729/150‑1 (MQ=255)

  aaaCCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAg                                                                                                                                               <  2:2416252/150‑1 (MQ=255)

  aaaCCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTACATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAg                                                                                                                                               <  2:1919834/150‑1 (MQ=255)

    aCCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAggg                                                                                                                                             >  2:3628501/1‑150 (MQ=255)

     ccGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGt                                                                                                                                            >  1:3336631/1‑150 (MQ=255)

     ccGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGt                                                                                                                                            >  2:2538204/1‑150 (MQ=255)

       gTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTAc                                                                                                                                          <  1:3527128/150‑1 (MQ=255)

       gTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTAc                                                                                                                                          <  1:3557689/150‑1 (MQ=255)

         tGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACtt                                                                                                                                        <  2:4931279/150‑1 (MQ=255)

           aCCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACtttt                                                                                                                                      <  2:3148366/150‑1 (MQ=255)

           aCCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACtttt                                                                                                                                      >  2:2733917/1‑150 (MQ=255)

              aTCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAt                                                                                                                                   >  2:289087/1‑150 (MQ=255)

              aTCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAt                                                                                                                                   <  1:2636473/150‑1 (MQ=255)

               tCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtg                                                                                                                                  <  2:356076/150‑1 (MQ=255)

                 aaCCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAtgtg                                                                                                                                <  2:3559748/150‑1 (MQ=255)

                  aCCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTgg                                                                                                                               <  2:84117/150‑1 (MQ=255)

                  aCCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTgg                                                                                                                               >  1:1214900/1‑150 (MQ=255)

                   ccTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGc                                                                                                                              <  2:1707507/150‑1 (MQ=255)

                     tgttgtATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGccc                                                                                                                            <  1:3611564/150‑1 (MQ=255)

                        tgtATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTg                                                                                                                         <  1:749051/150‑1 (MQ=255)

                           ataACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAg                                                                                                                      <  1:2619818/150‑1 (MQ=255)

                            taACACCTCCGATCTGCATAAACAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAgg                                                                                                                     <  2:3267240/150‑1 (MQ=255)

                            taACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAgg                                                                                                                     <  1:2069618/150‑1 (MQ=255)

                             aaCACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGc                                                                                                                    >  1:2289660/1‑150 (MQ=255)

                                  cTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgtg                                                                                                               >  2:1167013/1‑150 (MQ=255)

                                   tCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgtgt                                                                                                              <  1:4886276/150‑1 (MQ=255)

                                   tCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgtgt                                                                                                              >  1:1382097/1‑150 (MQ=255)

                                   tCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGgtgt                                                                                                              >  2:3354822/1‑150 (MQ=255)

                                    ccGATCTGGTTAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGtgtg                                                                                                             <  1:4496189/150‑1 (MQ=255)

                                      gATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCTTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCt                                                                                                           >  2:806929/1‑150 (MQ=255)

                                        tCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGa                                                                                                         >  1:2207672/1‑150 (MQ=255)

                                          tGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACt                                                                                                       >  1:3132427/1‑150 (MQ=255)

                                           gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACttt                                                                                                                                       <  2:3775341/117‑1 (MQ=255)

                                           gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACttt                                                                                                                                       >  1:3775341/1‑117 (MQ=255)

                                           gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTa                                                                                                      >  1:547285/1‑150 (MQ=255)

                                           gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTa                                                                                                      >  1:730516/1‑150 (MQ=255)

                                           gCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTa                                                                                                      <  2:730516/150‑1 (MQ=255)

                                            cATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTAc                                                                                                     <  1:4005069/150‑1 (MQ=255)

                                             aTAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACa                                                                                                    <  2:3730104/150‑1 (MQ=255)

                                             aTAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACa                                                                                                    <  1:2695651/150‑1 (MQ=255)

                                             aTAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACa                                                                                                    <  2:4487976/150‑1 (MQ=255)

                                               aaaaaGCTTGCTATTGCTTCCTCTTCATTGTTTAATAAAAACATTGGTGTATACGTCATTCTGGTTAACCTGGAGTTGATAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGTCTGGTGTGCTGACTACAAc                                                                                                  <  2:1132460/150‑1 (MQ=255)

                                                  aaGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGaa                                                                                               <  2:2205664/150‑1 (MQ=255)

                                                     cTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCa                                                                                            <  2:4092557/150‑1 (MQ=255)

                                                     cTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCa                                                                                            >  1:1169067/1‑150 (MQ=255)

                                                      tGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCaa                                                                                           >  1:155204/1‑150 (MQ=255)

                                                        gCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAAct                                                                                         <  2:1483953/150‑1 (MQ=255)

                                                        gCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAAct                                                                                         >  1:2758473/1‑150 (MQ=255)

                                                         cGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActt                                                                                        >  2:1217049/1‑150 (MQ=255)

                                                         cGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActt                                                                                        <  2:4974390/150‑1 (MQ=255)

                                                           aTTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActaac                                                                                      >  1:4516728/1‑147 (MQ=255)

                                                                tgcCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTtccttcct                                                                                 >  1:2882511/1‑150 (MQ=255)

                                                                 gcCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTg                                                                                <  2:1719679/150‑1 (MQ=255)

                                                                  ccTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGa                                                                               <  1:126121/150‑1 (MQ=255)

                                                                    tctTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAac                                                                             >  1:3910279/1‑150 (MQ=255)

                                                                        cATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCa                                                                         >  2:922435/1‑150 (MQ=255)

                                                                         aTTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCAt                                                                        <  2:308755/150‑1 (MQ=255)

                                                                            gtgGTAGTAAAACATTGGTGTTAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCt                                                                     <  2:2206621/150‑1 (MQ=255)

                                                                              tgaagaaAAACATTGGTGTAAACGACAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCttt                                                                   >  2:1863585/2‑150 (MQ=255)

                                                                               gaagaaAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTc                                                                  <  2:2120651/150‑1 (MQ=255)

                                                                               gaagaaAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTc                                                                  <  1:1883820/150‑1 (MQ=255)

                                                                                   aaaaaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAAc                                                              <  1:3027817/150‑1 (MQ=255)

                                                                                    aaaaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACa                                                             <  2:2302288/150‑1 (MQ=255)

                                                                                      aaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGc                                                           >  2:4748903/1‑150 (MQ=255)

                                                                                      aaCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGc                                                           <  1:4045853/150‑1 (MQ=255)

                                                                                       aCATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCt                                                          >  2:4345968/1‑150 (MQ=255)

                                                                                        ccttGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTc                                                         <  2:5004998/148‑1 (MQ=255)

                                                                                           tGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGTTGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCATCGTCCTTCCTGAACACCATGCTTTCGAACAGCTCGAt                                                      <  2:4652814/150‑1 (MQ=255)

                                                                                               gtAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAAt                                                  >  1:2505578/1‑150 (MQ=255)

                                                                                                tAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATa                                                 <  1:3662498/150‑1 (MQ=255)

                                                                                                tAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATa                                                 <  2:2034405/150‑1 (MQ=255)

                                                                                                  aaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAcc                                               >  1:2182763/1‑150 (MQ=255)

                                                                                                  aaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAcc                                               <  2:423288/150‑1 (MQ=255)

                                                                                                  aaCGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAcc                                               <  2:155204/150‑1 (MQ=255)

                                                                                                    tgTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACcgc                                             >  1:4760196/2‑150 (MQ=255)

                                                                                                    cGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACcgc                                             >  1:3870084/1‑150 (MQ=255)

                                                                                                     gTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACcgcg                                            >  1:1950676/1‑150 (MQ=255)

                                                                                                       cAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCa                                          <  2:1294207/150‑1 (MQ=255)

                                                                                                        aaaCTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCAt                                         >  2:1744599/1‑150 (MQ=255)

                                                                                                        aaaCTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCAt                                         <  1:1775199/150‑1 (MQ=255)

                                                                                                           cTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATtat                                      <  1:4170444/150‑1 (MQ=255)

                                                                                                           cTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATtat                                      <  2:4714524/150‑1 (MQ=255)

                                                                                                             ggTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATaa                                    >  2:480810/1‑150 (MQ=255)

                                                                                                                     aGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCgg                            >  2:3435710/1‑150 (MQ=255)

                                                                                                                      ggAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAAAAGCTCGATGAATACCGCGCATTATAAGAGCCCGGc                           >  2:1750542/1‑150 (MQ=255)

                                                                                                                          tGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCATGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCttt                       <  1:2950415/150‑1 (MQ=255)

                                                                                                                             aaaaCGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGAc                    >  2:756792/1‑150 (MQ=255)

                                                                                                                              aaaCGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACa                   <  1:476864/150‑1 (MQ=255)

                                                                                                                              aaaCGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACa                   >  2:669840/1‑150 (MQ=255)

                                                                                                                              aaaCGGTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCACCTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACa                   <  2:4279300/150‑1 (MQ=255)

                                                                                                                               aaCGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAg                  <  2:4036435/150‑1 (MQ=255)

                                                                                                                                   ttCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCAtt              <  2:2932263/150‑1 (MQ=255)

                                                                                                                                    tCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTa             <  1:3951661/150‑1 (MQ=255)

                                                                                                                                     ccTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTAt            >  2:1951087/1‑150 (MQ=255)

                                                                                                                                        cGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGc         >  2:555206/1‑150 (MQ=255)

                                                                                                                                          acacCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCgg       <  1:936715/150‑1 (MQ=255)

                                                                                                                                           cacCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTGCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGa      <  2:3944976/150‑1 (MQ=255)

                                                                                                                                             cccGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGaaa    >  1:5040491/1‑150 (MQ=255)

                                                                                                                                              ccGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAAc   <  2:5015601/150‑1 (MQ=255)

ACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACG  >  CP009273/1296583‑1296875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTG  >  CP009273/1296580‑1296878

CAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCNGNAGTGGAAAACGTTCCTCGACACCCGTA                                                                                                                                                       <  SRR19973063.1576067/150‑1 (MQ=60)

CAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTA                                                                                                                                                       >  SRR19973063.4057617/1‑150 (MQ=60)

 AGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAA                                                                                                                                                      <  SRR19973063.3053014/150‑1 (MQ=60)

   ACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAGACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACC                                                                                                                                                    <  SRR19973063.3937100/150‑1 (MQ=60)

       ACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGG                                                                                                                                                <  SRR19973063.3858301/150‑1 (MQ=60)

        CCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGT                                                                                                                                               >  SRR19973063.3347269/1‑150 (MQ=60)

        CCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGT                                                                                                                                               >  SRR19973063.3347223/1‑150 (MQ=60)

         CGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTA                                                                                                                                              <  SRR19973063.990995/150‑1 (MQ=60)

          GTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTAC                                                                                                                                             <  SRR19973063.3538422/150‑1 (MQ=60)

          GTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTAC                                                                                                                                             <  SRR19973063.3569093/150‑1 (MQ=60)

           TTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGANTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACT                                                                                                                                            <  SRR19973063.2682741/150‑1 (MQ=60)

           TTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACT                                                                                                                                            <  SRR19973063.5033968/150‑1 (MQ=60)

            TGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTT                                                                                                                                           >  SRR19973063.1899106/1‑150 (MQ=60)

                CATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTCCTGTTGA                                                                                                                                       >  SRR19973063.4400198/1‑150 (MQ=60)

                 ATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAT                                                                                                                                      >  SRR19973063.273130/1‑150 (MQ=60)

                 ATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAT                                                                                                                                      >  SRR19973063.273118/1‑150 (MQ=60)

                 ATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGAT                                                                                                                                      <  SRR19973063.2644887/150‑1 (MQ=60)

                   CAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGT                                                                                                                                    >  SRR19973063.2824599/1‑150 (MQ=60)

                     ACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGG                                                                                                                                  >  SRR19973063.1218540/1‑150 (MQ=60)

                        TGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCC                                                                                                                               <  SRR19973063.3623160/150‑1 (MQ=60)

                         GTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCG                                                                                                                              <  SRR19973063.5093281/150‑1 (MQ=60)

                           TGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTG                                                                                                                            <  SRR19973063.751238/150‑1 (MQ=60)

                           TGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTG                                                                                                                            <  SRR19973063.751198/150‑1 (MQ=60)

                           TGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTCAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTG                                                                                                                            <  SRR19973063.751188/150‑1 (MQ=60)

                              ATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAG                                                                                                                         <  SRR19973063.1283039/150‑1 (MQ=60)

                              ATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAG                                                                                                                         <  SRR19973063.2628159/150‑1 (MQ=60)

                               TAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGG                                                                                                                        <  SRR19973063.2076087/150‑1 (MQ=60)

                                AACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGC                                                                                                                       >  SRR19973063.2296971/1‑150 (MQ=60)

                                      TCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGT                                                                                                                 <  SRR19973063.4902036/150‑1 (MQ=60)

                                      TCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGT                                                                                                                 >  SRR19973063.1386273/1‑150 (MQ=60)

                                       CCGATCTGGTTAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTG                                                                                                                <  SRR19973063.4510618/150‑1 (MQ=60)

                                           TCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGA                                                                                                            >  SRR19973063.2214626/1‑150 (MQ=60)

                                           TCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGA                                                                                                            <  SRR19973063.4155453/150‑1 (MQ=60)

                                             TGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACT                                                                                                          >  SRR19973063.3142504/1‑150 (MQ=60)

                                              GCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTagatcggaagagcacacgtctgaactccagtca                                                                                                         >  SRR19973063.3787398/1‑117 (MQ=60)

                                              GCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTA                                                                                                         >  SRR19973063.548904/1‑150 (MQ=60)

                                              GCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTA                                                                                                         >  SRR19973063.732661/1‑150 (MQ=60)

                                              GCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTA                                                                                                         >  SRR19973063.2040763/1‑150 (MQ=60)

                                              GCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTA                                                                                                         >  SRR19973063.2649574/1‑150 (MQ=60)

                                               CATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTAC                                                                                                        >  SRR19973063.395112/1‑150 (MQ=60)

                                               CATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTAC                                                                                                        <  SRR19973063.4017914/150‑1 (MQ=60)

                                                ATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACA                                                                                                       <  SRR19973063.2704344/150‑1 (MQ=60)

                                                  AAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAAC                                                                                                     <  SRR19973063.371919/150‑1 (MQ=60)

                                                  AAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAAC                                                                                                     >  SRR19973063.3984314/1‑150 (MQ=60)

                                                   AAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAATATGGTTAACCAGGATTGGAAAACGTTCCTCGACACACGTAACCAGGGTACTTTTGATGGGGCACGTGCAGGCTGTTGTGATGACTACAACG                                                                                                    >  SRR19973063.3087400/1‑150 (MQ=60)

                                                   AAAAGCTGGCGATTGCTGCCCCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACG                                                                                                    <  SRR19973063.3306187/150‑1 (MQ=60)

                                                     AAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAA                                                                                                  <  SRR19973063.3015859/150‑1 (MQ=60)

                                                      AGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAAC                                                                                                 <  SRR19973063.4479783/150‑1 (MQ=60)

                                                      AGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAAC                                                                                                 >  SRR19973063.1241259/1‑150 (MQ=60)

                                                       GCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACC                                                                                                <  SRR19973063.79905/150‑1 (MQ=60)

                                                        CTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCA                                                                                               >  SRR19973063.1172588/1‑150 (MQ=60)

                                                        CTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCA                                                                                               >  SRR19973063.1172610/1‑150 (MQ=60)

                                                         TGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAA                                                                                              >  SRR19973063.155461/1‑150 (MQ=60)

                                                         TGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAA                                                                                              >  SRR19973063.155681/1‑150 (MQ=60)

                                                           GCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACT                                                                                            >  SRR19973063.2767368/1‑150 (MQ=60)

                                                              ATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTaac                                                                                         >  SRR19973063.4531226/1‑147 (MQ=60)

                                                              ATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCC                                                                                         >  SRR19973063.4531070/1‑150 (MQ=60)

                                                                TGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTT                                                                                       <  SRR19973063.3958634/150‑1 (MQ=60)

                                                                   TGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCT                                                                                    >  SRR19973063.2891726/1‑150 (MQ=60)

                                                                    GCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTG                                                                                   <  SRR19973063.1553327/150‑1 (MQ=60)

                                                                     CCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGA                                                                                  <  SRR19973063.126530/150‑1 (MQ=60)

                                                                      CTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAA                                                                                 >  SRR19973063.5106223/1‑150 (MQ=60)

                                                                       TCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAAC                                                                                >  SRR19973063.3922747/1‑150 (MQ=60)

                                                                       TCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAAC                                                                                >  SRR19973063.3923011/1‑150 (MQ=60)

                                                                       TCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAAC                                                                                >  SRR19973063.3922746/1‑150 (MQ=60)

                                                                         TTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACAC                                                                              >  SRR19973063.2106355/1‑150 (MQ=60)

                                                                            ATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCAT                                                                           <  SRR19973063.808014/150‑1 (MQ=60)

                                                                            ATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCAT                                                                           <  SRR19973063.2264244/150‑1 (MQ=60)

                                                                              TGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGC                                                                         <  SRR19973063.2633501/150‑1 (MQ=60)

                                                                               GTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCT                                                                        <  SRR19973063.4818728/150‑1 (MQ=60)

                                                                                TGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTT                                                                       <  SRR19973063.2225569/150‑1 (MQ=60)

                                                                                TGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTT                                                                       <  SRR19973063.2813689/150‑1 (MQ=60)

                                                                                  GAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTC                                                                     <  SRR19973063.1889707/150‑1 (MQ=60)

                                                                                      AAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAAC                                                                 <  SRR19973063.3037472/150‑1 (MQ=60)

                                                                                        AAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAG                                                               >  SRR19973063.4580870/1‑150 (MQ=60)

                                                                                         AACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGC                                                              <  SRR19973063.4058815/150‑1 (MQ=60)

                                                                                           CATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTC                                                            >  SRR19973063.3402217/1‑150 (MQ=60)

                                                                                           CATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTC                                                            >  SRR19973063.3402007/1‑150 (MQ=60)

                                                                                            ATTGGTGTAAACTTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACTAACCAACTTCCTTCCTGAACACCATTCTTTCGAACAGCTCG                                                           <  SRR19973063.4520265/150‑1 (MQ=60)

                                                                                            ATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCG                                                           >  SRR19973063.3696650/1‑150 (MQ=60)

                                                                                            ATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCG                                                           <  SRR19973063.25438/150‑1 (MQ=60)

                                                                                               GGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATG                                                        <  SRR19973063.3576517/150‑1 (MQ=60)

                                                                                                 TGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGTCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAA                                                      >  SRR19973063.4076785/1‑150 (MQ=60)

                                                                                                  GTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAAT                                                     >  SRR19973063.2513568/1‑150 (MQ=60)

                                                                                                   TAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATA                                                    >  SRR19973063.3788693/1‑150 (MQ=60)

                                                                                                   TAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATA                                                    <  SRR19973063.12782/150‑1 (MQ=60)

                                                                                                   TAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATA                                                    <  SRR19973063.3674239/150‑1 (MQ=60)

                                                                                                   TAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATA                                                    <  SRR19973063.3674223/150‑1 (MQ=60)

                                                                                                     AACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACC                                                  >  SRR19973063.2189598/1‑150 (MQ=60)

                                                                                                     AACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACC                                                  >  SRR19973063.1964356/1‑150 (MQ=60)

                                                                                                       TGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGC                                                >  SRR19973063.4775375/1‑150 (MQ=60)

                                                                                                       TGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGC                                                >  SRR19973063.4775474/1‑150 (MQ=60)

                                                                                                       CGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGC                                                >  SRR19973063.3882388/1‑150 (MQ=60)

                                                                                                        GTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACATCTCGATGAATACCGCG                                               >  SRR19973063.1956784/1‑150 (MQ=60)

                                                                                                           AAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCAT                                            >  SRR19973063.3552254/1‑150 (MQ=60)

                                                                                                           AAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCAT                                            >  SRR19973063.503171/1‑150 (MQ=60)

                                                                                                           AAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCAT                                            <  SRR19973063.1780673/150‑1 (MQ=60)

                                                                                                             ACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTA                                          <  SRR19973063.1857654/150‑1 (MQ=60)

                                                                                                              CTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTAT                                         <  SRR19973063.4183773/150‑1 (MQ=60)

                                                                                                               TGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATA                                        <  SRR19973063.356792/150‑1 (MQ=60)

                                                                                                                     ACCAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCC                                  >  SRR19973063.1900820/1‑150 (MQ=60)

                                                                                                                      CCAGGAGTTGAAAACGTTCCTCGACACCCGTAACCAGGTTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCC                                 <  SRR19973063.1838796/150‑1 (MQ=60)

                                                                                                                       CAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCG                                >  SRR19973063.638584/1‑150 (MQ=60)

                                                                                                                       CAGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCAGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCG                                <  SRR19973063.1235963/150‑1 (MQ=60)

                                                                                                                        AGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGG                               >  SRR19973063.4872313/1‑150 (MQ=60)

                                                                                                                        AGGAGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTACGAACAGCTCGATGAATACAGCGCATTATAAGAGCCCGG                               >  SRR19973063.2536543/1‑150 (MQ=60)

                                                                                                                           AGTGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCT                            <  SRR19973063.3070405/150‑1 (MQ=60)

                                                                                                                             TGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCATGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTT                          <  SRR19973063.2959845/150‑1 (MQ=60)

                                                                                                                             TGGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTT                          <  SRR19973063.364679/150‑1 (MQ=60)

                                                                                                                              GGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTG                         <  SRR19973063.4653534/150‑1 (MQ=60)

                                                                                                                              GGAAAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTG                         >  SRR19973063.1752737/1‑150 (MQ=60)

                                                                                                                                 AAACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACA                      <  SRR19973063.478214/150‑1 (MQ=60)

                                                                                                                                  AACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAG                     <  SRR19973063.20791/150‑1 (MQ=60)

                                                                                                                                  AACGTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAG                     <  SRR19973063.3989713/150‑1 (MQ=60)

                                                                                                                                     TTTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCAT                  <  SRR19973063.2494242/150‑1 (MQ=60)

                                                                                                                                      TTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATT                 >  SRR19973063.4056482/1‑150 (MQ=60)

                                                                                                                                      TTCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATT                 <  SRR19973063.3915496/150‑1 (MQ=60)

                                                                                                                                       TCCTCGACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTA                <  SRR19973063.3964298/150‑1 (MQ=60)

                                                                                                                                            GACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCG           <  SRR19973063.289941/150‑1 (MQ=60)

                                                                                                                                            GACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCG           <  SRR19973063.290017/150‑1 (MQ=60)

                                                                                                                                             ACACCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGG          <  SRR19973063.939507/150‑1 (MQ=60)

                                                                                                                                                CCCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAA       >  SRR19973063.5056733/1‑150 (MQ=60)

                                                                                                                                                 CCGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAAC      >  SRR19973063.2385826/1‑150 (MQ=60)

                                                                                                                                                  CGTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACG     <  SRR19973063.2207891/150‑1 (MQ=60)

                                                                                                                                                   GTAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGC    >  SRR19973063.2126462/1‑150 (MQ=60)

                                                                                                                                                    TAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCT   >  SRR19973063.1547907/1‑150 (MQ=60)

                                                                                                                                                    TAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCT   >  SRR19973063.3146464/1‑150 (MQ=60)

                                                                                                                                                    TAACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCT   >  SRR19973063.4100464/1‑150 (MQ=60)

                                                                                                                                                     AACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTG  <  SRR19973063.2882439/150‑1 (MQ=60)

CAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTG  >  CP009273/1296580‑1296878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`